Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pBT100.110 CDA1-intein SapI Golden Gate donor
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    aatgctcttctagtctactacataaatcaaacataaggaggtaaccatgaccgacgcggaatacgttcgtatccacgaaaaactggacatctacaccttcaaaaaacagttcttcaacaacaaaaaatctgtttctcaccgttgctacgttctgttcgaactgaaacgtcgtggtttacgagaagatcagatgatgtatttagtttgtattcctccattggtactggctgcgccttatgcaagcataggtgctttttgacctgtagatgtggaagttttttgtcaagaagttgttgttttttagacaaagagtggcaacgatgcaagacaagcttgactttgcagcaccaAccIBaeISapIXmnIBspQIBstBIBstEIIMT2DA4EY6VR8IH10EK12LD14IY16TF18KK20QF22FN24NK26KS28VS30HR32CY34VL36FE38LK40RR42G47-670pmCDA1syn RBS 654k ... from proIII20406080100120140160
    gaacgtcgtgcgtgcttctggggttacgcggttaacaaaccgcagtctggtaccgaacgtggtatccacgcggaaatcttctctatccgtaaagttgaagaatacctgcgtgacaacccgggtcagttcaccatcaactggtactcttcttggtctccgtgcgcggactgcgcggcttgcagcacgcacgaagaccccaatgcgccaattgtttggcgtcagaccatggcttgcaccataggtgcgcctttagaagagataggcatttcaacttcttatggacgcactgttgggcccagtcaagtggtagttgaccatgagaagaaccagaggcacgcgcctgacgcgccAloIAvaIBanIBsaIHpaIKpnISmaIXmaIBcoDIBfuAIBsmAIBsoBIBspMIPflMITspMIAcc65IHincIIE44RR46AC48FW50GY52AV54NK56PQ58SG60TE62RG64IH66AE68IF70SI72RK74VE76EY78LR80DN82PG84QF86TI88NW90YS92SW94SP96CA98DC100A47-670pmCDA1180200220240260280300320340
    aaaaaatcctggaatggtacaaccaggaactgcgtggtaacggtcacaccctgaaaatctgggcgtgcaaactgtactacgaaaaaaacgcgcgtaaccagatcggtctgtggaacctgcgtgacaacggtgttggtctgaacgttatggtttctgaacactaccagtgctgccgttttttaggaccttaccatgttggtccttgacgcaccattgccagtgtgggacttttagacccgcacgtttgacatgatgctttttttgcgcgcattggtctagccagacaccttggacgcactgttgccacaaccagacttgcaataccaaagacttgtgatggtcacgacggcAclIPfoITatIXcmIBfuAIBspMIE102KI104LE106WY108NQ110EL112RG114NG116HT118LK120IW122AC124KL126YY128EK130NA132RN134QI136GL138WN140LR142DN144GV146GL148NV150MV152SE154HY156QC158CR16047-670pmCDA1360380400420440460480500520
    taaaatcttcatccagtcttctcacaaccagctgaacgaaaaccgttggctggaaaaaaccctgaaacgtgcggaaaaacgtcgttctgaactgtctatcatgatccaggttaaaatcctgcacaccaccaaatctccggcggtttccggtagcgaaacaccggggacttcagaaattttagaagtaggtcagaagagtgttggtcgacttgcttttggcaaccgaccttttttgggactttgcacgcctttttgcagcaagacttgacagatagtactaggtccaattttaggacgtgtggtggtttagaggccgccaaaggccatcgctttgtggcccctgaagtcttBbsIBsgIBsaXIPvuIIKI162FI164QS166SH168NQ170LN172EN174RW176LE178KT180LK182RA184EK186RR188SE190LS192IM194IQ196VK198IL200HT202TK204SP206AV20847-670S1G2S3E4T5P6G7T8S9E10671-715pmCDA1XTEN linker540560580600620640660680700
    tcggccaccccggAgtgcctgTcatacgaaaccgagatactgacagtagaatatggccttctgccaatcgggaagattgtggagaaacggatagaatgcacagtttactctgtcgataacaatggtaacatttatactcagccagttgcccagtggcacgaccggggagagcaggagccggtggggccTcacggacAgtatgctttggctctatgactgtcatcttataccggaagacggttagcccttctaacacctctttgcctatcttacgtgtcaaatgagacagctattgttaccattgtaaatatgagtcggtcaacgggtcaccgtgctggcccctctcgtccBmrIEaeIXcmIBsaXIBsmFICL2SY4ET6EI8LT10VE12YG14LL16PI18GK20IV22EK24RI26EC28TV30YS32VD34NN36GN38IY40TQ42PV44AQ46WH48DR50GE52Q716-1024S11A12T13P14E15671-715Npu DnaE N-term inteinXTEN...ker720740760780800820840860
    aagtattcgaatactgtctggaggatggaagtctcattagggccactaaggaccacaaatttatgacagtcgatggccagatgctgcctatagacgaaatctttgagcgagagttggacctcatgcgagttgacaaccttcctaattaaggcggctgaagagcctggttaaaaaattcataagcttatgacagacctcctaccttcagagtaatcccggtgattcctggtgtttaaatactgtcagctaccggtctacgacggatatctgctttagaaactcgctctcaacctggagtacgctcaactgttggaaggattaattccgccgacttctcggaccaattttttBpmIEaeIMscISapIAvaIIAvaIIBcoDIBsmAIBspQIBstBIEcoNIHincIIE54VF56EY58CL60ED62GS64LI66RA68TK70DH72KF74MT76VD78GQ80ML82PI84DE86IF88ER90EL92DL94MR96VD98NL100PN102*716-1024Npu DnaE N-term intein8809009209409609801,0001,0201,040
    tgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaaactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtacttaattaagaatctttttgagtagctcgtagtttAseISspIXmnIBsrBICspCI*272FF270ED268LM266L1201-2016aph (KanR)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagttAseIPflMIH264FQ262LK260NM258DP256ND254IG252YK250QF248LR246KQ244LS242PS240FE238GL236CN234WL232IA230LD228QY226RD224AI222GV220RG218VD216IC214GI212LK210GE208DF2061201-2016aph (KanR)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagccagacgtttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcggtctgcAjuINsiIBpu10IIL204ND202LS200FD198GH196TV194VS192DP190SF188PL186LK184HM182EK180WV178QE176VP174WG172NR170ED168DF166DS164AD162VL160GN158NM156RS154QA152QA150LR1481201-2016aph (KanR)1,4201,4401,4601,4801,5001,5201,5401,560
    aaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtatttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatAvaIBtsIBtsINsiIPvuISmaISspIXmaIAsiSIBsoBIBsrFIBtsαIBtsαIEcoNITspMIFV146RD144SN142FP140CN138CV136PI134SH132LR130RL128FV126AL124AD122VI120NE118GS116DP114YE112EL110VQ108FA106TK104GP102IA100TT98LL96WA94DD92PT901201-2016aph (KanR)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaataClaIBspDIRI88FH86KI84TP82LP80MF78ET76LW74NL72RV70ME68DT66VD64NA62VS60GK58GH56KL54FL52EP50AD48PK46GY44LR42YI40TA38GS36QG34VN32D1201-2016aph (KanR)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactggttttagggaattgcactcaaaagcaaggtgaNruIBanIIBsp1286IR30AW28KY26GY24LD22AD20MN18SN16LR14PR12SC10ST8ER6QI4HS2M1201-2016aph (KanR)pUC origin high copy1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccctcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggpUC origin high copy2,1202,1402,1602,1802,2002,2202,2402,260
    aaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcpUC origin high copy2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtcEciIApaLIBaeGIBseYIBssSIHaeIIBssSαIBsiHKAIBsp1286IpUC origin high copy2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttcccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaaDrdIEciINspIPciIAflIIIpUC origin high copypredicted terminator2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgagtaggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctcBsrBI2,8202,8402,8602,880
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    2897 bppBT100 backbone2000225025002750250500SapI716-102447-670671-715

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    2897