Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
XZ088 (IVT) CMV-TO-T7-CCR6-V2-ADAR2dd-hybridUTR3
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggpUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIAmp (bla)180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspINmeAIIIAmp (bla)360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIXmnIAmp (bla)720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoter8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactSgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIAlwNIBsiWIBpu10I1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)1,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaNdeICMVCMV promoter (with TetOs)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggNcoICspCISnaBICMVCMV promoter (with TetOs)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagctcgctagagatccgcggccgctaatacgactcactatagggagaaaaataacaaatctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttaaatcatgccctggctaggtcggaggcctgagatcgcaaatttgaattcgagcgatctctaggcgccggcgattatgctgagtgatatccctctttttattgtttagagttgtgttgtatatgttttgtttgcttagagttcgttagttcgtaagatgaagataacgtcgttaaatttagtaBsmINotIPmeISwaIAflIISacIITEV leader UTR5IVT primer bind siteT7 insertCMV pro...TetOs)homolog2,1202,1402,1602,1802,2002,2202,2402,260
    ttcttttaaagcaaaagcaattttctgaaaattttcaccatttacgaacgataAAGCTTcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatATGAGCGGAGAGAGCATGAACTTCAGTGaagaaaatttcgttttcgttaaaagacttttaaaagtggtaaatgcttgctatTTCGAAgcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctaTACTCGCCTCTCTCGTACTTGAAGTCACClaIXmnIBspDIBpu10IHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28SG30ES32MN34FS362342-4855Signal/FlagTEV leader UTR5CCR6homolog2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ATGTGTTCGATTCCAGTGAGGACTACTTCGTGTCCGTCAATACATCCTACTACAGCGTAGATTCTGAGATGCTGCTTTGCTCCCTGCAGGAGGTGCGCCAGTTCTCCCGACTCTTCGTTCCCATCGCGTATTCACTCATCTGCGTCTTTGGCCTCCTGGGGAATATCTTGGTAGTTACACAAGCTAAGGTCACTCCTGATGAAGCACAGGCAGTTATGTAGGATGATGTCGCATCTAAGACTCTACGACGAAACGAGGGACGTCCTCCACGCGGTCAAGAGGGCTGAGAAGCAAGGGTAGCGCATAAGTGAGTAGACGCAGAAACCGGAGGACCCCTTATAGAACCATCASbfIDV38FD40SS42ED44YF46VS48VN50TS52YY54SV56DS58EM60LL62CS64LQ66EV68RQ70FS72RL74FV76PI78AY80SL82IC84VF86GL88LG90NI92LV94V2342-4855CCR62,4602,4802,5002,5202,5402,5602,5802,6002,620
    CATCACATTTGCTTTTTATAAAAAGGCCAGATCCATGACAGACGTCTACCTGCTTAATATGGCCATTGCAGACATCCTGTTCGTCCTGACGCTCCCATTCTGGGCTGTGAGTCACGCTACCGGGGCCTGGGTGTTCAGTAATGCAACCTGCAAGCTGTTGAAGGGAATTTATGCCGTAGTGTAAACGAAAAATATTTTTCCGGTCTAGGTACTGTCTGCAGATGGACGAATTATACCGGTAACGTCTGTAGGACAAGCAGGACTGCGAGGGTAAGACCCGACACTCAGTGCGATGGCCCCGGACCCACAAGTCATTACGTTGGACGTTCGACAACTTCCCTTAAATACGGPsiII96TF98AF100YK102KA104RS106MT108DV110YL112LN114MA116IA118DI120LF122VL124TL126PF128WA130VS132HA134TG136AW138VF140SN142AT144CK146LL148KG150IY152A2342-4855CCR62,6402,6602,6802,7002,7202,7402,7602,7802,800
    ATCAACTTTAATTGCGGTATGCTGCTGCTGACTTGCATAAGTATGGACAGATACATCGCCATAGTACAGGCAACAAAATCCTTCAGACTGAGGTCTCGAACGCTCCCTAGGTCTAAAATCATTTGTCTTGTTGTATGGGGCTTGAGCGTGATTATTTCCAGCAGTACTTTCGTGTTAGTTGAAATTAACGCCATACGACGACGACTGAACGTATTCATACCTGTCTATGTAGCGGTATCATGTCCGTTGTTTTAGGAAGTCTGACTCCAGAGCTTGCGAGGGATCCAGATTTTAGTAAACAGAACAACATACCCCGAACTCGCACTAATAAAGGTCGTCATGAAAGCACABsaIAvrIII154NF156NC158GM160LL162LT164CI166SM168DR170YI172AI174VQ176AT178KS180FR182LR184SR186TL188PR190SK192II194CL196VV198WG200LS202VI204IS206SS208TF210V2342-4855CCR62,8202,8402,8602,8802,9002,9202,9402,960
    TTAACCAGAAGTACAACACGCAGGGCAGCGATGTGTGTGAGCCCAAGTACCAGACGGTATCTGAGCCTATCAGGTGGAAGCTGTTGATGCTGGGTTTGGAATTGCTGTTCGGCTTTTTTATCCCACTCATGTTTATGATTTTTTGTTATACCTTCATCGTGAAGACCCTGGTCCAAATTGGTCTTCATGTTGTGCGTCCCGTCGCTACACACACTCGGGTTCATGGTCTGCCATAGACTCGGATAGTCCACCTTCGACAACTACGACCCAAACCTTAACGACAAGCCGAAAAAATAGGGTGAGTACAAATACTAAAAAACAATATGGAAGTAGCACTTCTGGGACCAGGTAloIF212NQ214KY216NT218QG220SD222VC224EP226KY228QT230VS232EP234IR236WK238LL240ML242GL244EL246LF248GF250FI252PL254MF256MI258FC260YT262FI264VK266TL268VQ2702342-4855CCR62,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGCACAAAACTCTAAGCGACACAAGGCCATCCGGGTAATCATCGCTGTTGTGCTGGTATTTCTGGCTTGCCAGATCCCTCATAATATGGTCCTTCTGGTAACGGCAGCCAACCTGGGCAAAATGAATCGCTCATGTCAGTCAGAAAAGCTTATCGGGTACACCAAAACGGTTACGCCGTGTTTTGAGATTCGCTGTGTTCCGGTAGGCCCATTAGTAGCGACAACACGACCATAAAGACCGAACGGTCTAGGGAGTATTATACCAGGAAGACCATTGCCGTCGGTTGGACCCGTTTTACTTAGCGAGTACAGTCAGTCTTTTCGAATAGCCCATGTGGTTTTGCCAATGCHindIIIAQ272NS274KR276HK278AI280RV282II284AV286VL288VF290LA292CQ294IP296HN298MV300LL302VT304AA306NL308GK310MN312RS314CQ316SE318KL320IG322YT324KT326VT3282342-4855CCR63,1603,1803,2003,2203,2403,2603,2803,3003,320
    GAGGTTCTGGCGTTCCTGCATTGTTGCCTTAACCCTGTGCTGTACGCATTTATAGGTCAGAAGTTTCGAAACTACTTCCTGAAGATTCTCAAAGACCTCTGGTGTGTAAGAAGAAAGTATAAAAGTAGTGGATTTTCATGCGCAGGACGCTACTCCGAAAACATTTCCAGACAGACTCCAAGACCGCAAGGACGTAACAACGGAATTGGGACACGACATGCGTAAATATCCAGTCTTCAAAGCTTTGATGAAGGACTTCTAAGAGTTTCTGGAGACCACACATTCTTCTTTCATATTTTCATCACCTAAAAGTACGCGTCCTGCGATGAGGCTTTTGTAAAGGTCTGTCTFspIBstBIEV330LA332FL334HC336CL338NP340VL342YA344FI346GQ348KF350RN352YF354LK356IL358KD360LW362CV364RR366KY368KS370SG372FS374CA376GR378YS380EN382IS384RQ3862342-4855CCR63,3403,3603,3803,4003,4203,4403,4603,4803,500
    CCAGTGAGACGGCCGATAACGATAACGCCTCCTCCTTTACCATGaccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCGGTCACTCTGCCGGCTATTGCTATTGCGGAGGAGGAAATGGTACtggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGAgeIAgeIBsgIKpnIXcmIAcc65ITS388ET390AD392ND394NA396SS398FT400MT402GG404RT406PP408SL410GP412QD414ES416CT418TA420SS422SL424AK426DT428SS430GT432GA434LH436LD438QT440PS442RQ444P2342-4855V2 tailCCR6hADAR2 cat domainhomologhomolog3,5203,5403,5603,5803,6003,6203,6403,660
    TATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCBstEIII446PS448EG450LQ452LH454LP456QV458LA460DA462VS464RL466VL468GK470FG472DL474TD476NF478SS480PH482AR484RK486VL488AG490VV492MT494TG496TD498VK500DA502K2342-4855hADAR2 cat domain3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGABclIV504IS506VS508TG510TK512CI514NG516EY518MS520DR522GL524AL526ND528CH530AE532II534SR536RS538LL540RF542LY544TQ546LE548LY550LN552NK554DD556QK558RS560I2342-4855hADAR2 cat domain3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTAPfoIBsrGIF562QK564SE566RG568GF570RL572KE574NV576QF578HL580YI582ST584SP586CG588DA590RI592FS594PH596EP598IL600EE602PA604DR606HP608NR610KA612RG614QL616RT618KI6202342-4855hADAR2 cat domain4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGBlpIBamHIES622GQ624GT626IP628VR630SN632AS634IQ636AW638DG640VL642QG644ER646LL648TM650SC652SD654KI656AR658WN660VV662GI664QG666SL668LS670IF672VE674PI676YF6782342-4855hADAR2 cat domain4,2204,2404,2604,2804,3004,3204,3404,360
    TCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTliIXhoIPaeR7ISS680II682LG684SL686YH688GD690HL692SR694AM696YQ698RI700SN702IE704DL706PP708LY710TL712NK714PL716LS718GI720SN722AE724AR726QP728GK730AP732NF734SV7362342-4855hADAR2 cat domain4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTMluINW738TV740GD742SA744IE746VI748NA750TT752GK754DE756LG758RA760SR762LC764KH766AL768YC770RW772MR774VH776GK778VP780SH782LL784RS786KI788TK790PN792VY794H2342-4855hADAR2 cat domain4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgaCTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactGAGCTCGACCATGACGTACGTGCGTTACGATCGACGGGGAAAGGGBmtINheISphITliIXhoIPspXIPaeR7IE796SK798LA800AK802EY804QA806AK808AR810LF812TA814FI816KA818GL820GA822WV824EK826PT828EQ830DQ832FS834LT836P*8382342-4855hADAR2 cat domainhybrid UTR3homolog4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCACAGGACCCATGGGGCTCAGAGGGGGCTGGAGCCCAGGGTCCATACGAGGGTGGAGGTGGACGGGGTGAGTGGTGGAGACGATCAAGGTCTGTGGAGGGTTCGTGCGTCGTTACGTCGAGTTTTGCGAATCGGATCGGTGTGGGGGTGCCCTTTGTCGTCACTAATTGGAAATCGTAhdIBlpIKpnIPaqCIAcc65Ihybrid UTR34,9204,9404,9604,9805,0005,0205,0405,060
    ATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgTATTTGCTTTCAAATTGATTCGATATGATTGGGGTCCCAACCAGTTAAAGCACGGTCGGTGTGGGACCTCGATCGcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacApaIBclIBmtINheIPasIPmeIXcmIPflMIPspOMIhybrid UTR3c-myc epitopeIVT pr... sitePolyhi...e taghomologBG...e5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggaacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctBGH polyadenylation sequence5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ttgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaaClaISphIBspDIBGH polyaden...on sequenceBGH pA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    acgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccNaeINgoMIV5,6205,6405,6605,6805,7005,7205,7405,760
    tgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcaBarIXbaIHygromycinFRT5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgcccgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggEcoRIHygromycin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacNcoINdeIPvuIAsiSIRsrIIDraIIIHygromycin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgDraIIINmeAIIIHygromycin6,3206,3406,3606,3806,4006,4206,4406,460
    ataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcatattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtAjuISacIIHygromycin6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ttggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgaaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctHygromycin6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggNaeIPfoINgoMIVHygromycin6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtaBsmIPsiIBstZ17ISV40 pA7,0207,0407,0607,0807,1007,1207,1407,160
    agctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaattcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattactta7,1807,2007,2207,2407,2607,2807,3007,3207,340
    cggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttPciISapIBspQIpUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacpUC ori7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactaaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatpUC ori7,7207,7407,7607,7807,8007,8207,8407,860
    tcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaaAlwNIpUC ori7,8807,9007,9207,9407,9607,9808,0008,0208,040
    accttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori8,0608,0808,1008,1208,1408,160
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8161 bp6000650070007500500100015002000CMV promoter (with TetOs)CMVTetR binding sitesTetOXZp018.4 (DNA)pNK018 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),AhdI,BsaIAmp (bla),NmeAIII,FspI,AclIPvuIAclI,XmnISspIbla promoter,SgrDIFlpInF1 (DNA),AlwNIFlpInF (DNA),EKo152.4 (DNA),MfeI,Bpu10I,BsiWI,MluISpeINdeISnaBINcoI,CspCIA,CMV-Forward Genewiz (DNA)TATA box,TetO,Eco53kI,SacI,BsaBI,BsaBI,SgrDI,Eco53kI,+1DraIII,NcoIAsiSI,PvuI,RsrII,NdeIHygromycinNmeAIII,DraIIIAjuISacIIHyg_F (DNA)PfoI,NgoMIV,NaeISV40 pA,SV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiI,BsmIBstZ17IM13R Genewiz (DNA)pUCori5F (DNA),BspQI,SapI,PciIpUCoriR (DNA)pUC ori,AlwNI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    8161