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pCrU6.4-SpCas9_cloning/aphVIII
  • Sequence Map
    Editing disabled on read-only sequence.
    gatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccNaeIBsaAIBtgZINgoMIVF1 ori20406080100120140160
    caagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaaggttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcFspILacZ alphaF1 ori180200220240260280300320340
    ttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagctccaccgcggtggcggccgctctagaactagtggatcccccgggctgcTCTTTGACGAGCGCTATCACGCTGTGCCATTGGTGGTCaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacctcgaggtggcgccaccgccggcgagatcttgatcacctagggggcccgacgAGAAACTGCTCGCGATAGTGCGACACGGTAACCACCAGAfeIAleIEagINotISacISmaISpeIXbaIXmaIBamHIBstXITspMIEco53kIPU6.4T7Fragme...fwd))M13-fwd360380400420440460480500520
    CTGTTCTGGAAATCCATGGAAATCCCCTCGACGCAGGCCACCCCGACACCACAGCCAAGCGCAGGTCTGCTGGGCTCTGCGAAATCGAATGCAAGTTTAACTGGTGCGCGCGTTCCCTGGACCCTGATTGGCCCATTTTAGAACATTCATGAAGTTGATATACTGCAGATATGCGGACAAGACCTTTAGGTACCTTTAGGGGAGCTGCGTCCGGTGGGGCTGTGGTGTCGGTTCGCGTCCAGACGACCCGAGACGCTTTAGCTTACGTTCAAATTGACCACGCGCGCAAGGGACCTGGGACTAACCGGGTAAAATCTTGTAAGTACTTCAACTATATGACGTCTATACGCBsmIPstIPU6.4540560580600620640660680700
    AGAGATAATAAACTAAGCAGTCGGAGGTCGGCCATGCCTCGTGACAGCGGCGTCAACTCCGAGTTCCTGCGGTTCCTGTAGCGCATGAGCATGGGTCGCGTGTGGTGATGACCAGGAACGGCGGCGGCAGGAAGCGGAAGAGGAGGAGGTCAGGAGCAAGAGCtAAGCAGGTAGGTCTCTATTATTTGATTCGTCAGCCTCCAGCCGGTACGGAGCACTGTCGCCGCAGTTGAGGCTCAAGGACGCCAAGGACATCGCGTACTCGTACCCAGCGCACACCACTACTGGTCCTTGCCGCCGCCGTCCTTCGCCTTCTCCTCCTCCAGTCCTCGTTCTCGaTTCGTCCATCCPU6.4720740760780800820840860
    AGAACGAGGGAGCAGAGTACTTGTGGCTGGGAGGCCACCGGTGCCCTAGCAGGGGCACGCGCCGGTGACGTGAGCAGGAGGAGAAGGCAGTATACGCACTTGACACGGCTTCAGGAGGCGCACAGGAGGCGGAGGCCGCCACGAACCAGCAGAGGAAGCAATAAATTGTCTTACTTCTTGCTCCCTCGTCTCATGAACACCGACCCTCCGGTGGCCACGGGATCGTCCCCGTGCGCGGCCACTGCACTCGTCCTCCTCTTCCGTCATATGCGTGAACTGTGCCGAAGTCCTCCGCGTGTCCTCCGCCTCCGGCGGTGCTTGGTCGTCTCCTTCGTTATTTAACAGAATGAAgeIScaITatISgrAISgrAIBstZ17IPU6.48809009209409609801,0001,0201,040
    ATATATATATTTGGCCTATGAGAATTactttgagacgtaccatatgaaccgtctccgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttTATCTTTCTAGAAGATCTCCTACAATATTCTCAGCTGTATATATATAAACCGGATACTCTTAAtgaaactctgcatggtatacttggcagaggcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaATAGAAAGATCTTCTAGAGGATGTTATAAGAGTCGACNdeIXbaIBglIIBsmBIBsmBIEsp3IEsp3ISpCas9 ScaffoldPU6.4Cloning Insert1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CCATGGAAAATCGATGTTCTTCTTTTATTCTCTCAAGATTTTCAGGCTGTATATTAAAACTTATATTAAGAACTATGCTAACCACCTCATCAGGAACCGTTGTAGGTGGCGTGGGTTTTCTTGGCAATCGACTCTCATGAAAACTACGAGCTAAATATTCAATATGTTCCTCTTGGGTACCTTTTAGCTACAAGAAGAAAATAAGAGAGTTCTAAAAGTCCGACATATAATTTTGAATATAATTCTTGATACGATTGGTGGAGTAGTCCTTGGCAACATCCACCGCACCCAAAAGAACCGTTAGCTGAGAGTACTTTTGATGCTCGATTTATAAGTTATACAAGGAGAACClaIBspDI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ACCAACTTTATTCTGCATTTTTTTTGAACGAGGTTTAGAGCAagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgTGGTTGAAATAAGACGTAAAAAAAACTTGCTCCAAATCTCGTtcgaacgtacggcccgcgcggtcttcctcgcgtcggtttggtcctactacaaactaccccataaactcgtgaacgttgggaataggccttcgggggaccgggtgtttccgatccgcggttacgttcgtcaagcAscIKasINarISfoISphIBspEIPluTIHindIIIRBCS2 Promotor1,4201,4401,4601,4801,5001,5201,5401,560
    catgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaaatggccaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcggtacgtcggggacctcgccacgggaggactatttggccggtcccccggatacaagaaatgaaaaaatgttctcttcagtgagttgtagaattttaccggtccactcagctgctcgttcgggccgcctagtccgtcgcacgaacgtctaaactgaacgttgcgggcgtaacacagcMscISphIIn1Intron 1RbcS2 Intron1RBCS2 Promotor5´UTR RBCS21,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcaggatgctcgagattcgaagcatggacgatgcgttgcgtgcactgcggggtcggtatcccggttgtgagtgggttgttgtggaggatggggcctcgggggctggtgtgcttccgaaaaccgaggagacagcgacagagttcgtcgtagattgggacgcagcggcaaaggtaaacgtcctacgagctctaagcttcgtacctgctacgcaacgcacgtgacgccccagccatagggccaacactcacccaacaacacctcctaccccggagcccccgaccacTliIXhoIBstBIPaeR7IAPHVIIIIn1Intron 1RbcS2 Intron11,7601,7801,8001,8201,8401,8601,8801,9001,920
    tttatcggcttcggggtggtgggcgggagttgtttgtcaaggtggcagctctgggggccggggtgggcttgttgggtgaggctgagcggctggtgtggttggcggaggtggggattcccgtacctcgtgttgtggagggtggtggggacgagagggtcgcctggttggtcaccgaaaatagccgaagccccaccacccgccctcaacaaacagttccaccgtcgagacccccggccccacccgaacaacccactccgactcgccgaccacaccaaccgcctccacccctaagggcatggagcacaacacctcccaccacccctgctctcccagcggaccaaccagtggctAPHVIII1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agcggttccggggcgtccggccagtgcgcggtggccgcgggagcagcggctggacgtggcggtggcgctcgcggggctcgctcgttcgctgcacgcgctggactgggagcggtgtccgttcgatcgcagtctcgcggtgacggtgccgcaggcggcccgtgctgtcgctgaagggtcgccaaggccccgcaggccggtcacgcgccaccggcgccctcgtcgccgacctgcaccgccaccgcgagcgccccgagcgagcaagcgacgtgcgcgacctgaccctcgccacaggcaagctagcgtcagagcgccactgccacggcgtccgccgggcacgacagcgacttcccBsgIXmnIAPHVIII2,1202,1402,1602,1802,2002,2202,2402,260
    agcgtcgacttggaggatctggacgaggagcggaaggggtggtcgggggagcggcttctcgccgagctggagcggactcggcctgcggacgaggatctggcggtttgccacggtgacctgtgcccggacaacgtgctgctcgaccctcgtacctgcgaggtgaccgggctgatcgtcgcagctgaacctcctagacctgctcctcgccttccccaccagccccctcgccgaagagcggctcgacctcgcctgagccggacgcctgctcctagaccgccaaacggtgccactggacacgggcctgttgcacgacgagctgggagcatggacgctccactggcccgactagcBaeIAPHVIII2,2802,3002,3202,3402,3602,3802,4002,4202,440
    acgtggggcgggtcggccgtgcggaccggcactccgatctcgcgctggtgctgcgcgagctggcccacgaggaggacccgtggttcgggccggagtgttccgcggcgttcctgcgggagtacgggcgcgggtgggatggggcggtatcggaggaaaagctggcgttttaccggcttgcaccccgcccagccggcacgcctggccgtgaggctagagcgcgaccacgacgcgctcgaccgggtgctcctcctgggcaccaagcccggcctcacaaggcgccgcaaggacgccctcatgcccgcgcccaccctaccccgccatagcctccttttcgaccgcaaaatggccgaAloIEagIPpuMIRsrIIAPHVIII2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gttggacgagttcttctgagggacctgatggtgttggtggctgggtagggttgcgtcgcgtgggtgacagcacagtgtggacgttgggatccccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagcaacctgctcaagaagactccctggactaccacaaccaccgacccatcccaacgcagcgcacccactgtcgtgtcacacctgcaaccctaggggcgaggcacatttacctccgcgagcaactagactcggaacgggggactgcttgccgccacctaccttctatgacgagagttcBamHIPpuMIRbcs2 Term.Rbcs2\Term.APHVIII2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcgggtacccagcttttgttccctttaacgacttcgccatcgaatcgaggggcaaagcacgactagtcagaaaaagttgtgcatttttcgcctcctcaaaacgttaaaacaaccaacattgctaggaggcaactaaaaccggagaaagaggtacccgcccgacccgcataaacttcgcccatgggtcgaaaacaagggaaatBclIKpnIXcmIBsaAIAcc65IAflIIIRbcs2 Term.Rbcs2\Term.Terminator HairpinT32,8202,8402,8602,8802,9002,9202,9402,960
    gtgagggttaattGCGCgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccactcccaattaaCGCGcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaglac promoterLacOM13-revT3 ...erT32,9803,0003,0203,0403,0603,0803,1003,1203,140
    cagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcSapIBspQI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccattagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtPciIAflIIIColE1 origin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacccccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttgggColE1 origin3,5203,5403,5603,5803,6003,6203,6403,660
    cccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttColE1 origin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgcccColE1 origin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagColE1 originAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtAhdIBsaIBsrDIAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    gaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatccttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagAclIFspIBsrDIAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtacttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgScaITatIAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacaAclIXmnIAmpR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgttgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataac4,9204,9404,9604,9805,0005,0205,0405,060
    tctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaaagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttApoIApoICspCI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgAloIPsiIF1 ori5,2605,2805,3005,3205,3405,360
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5372 bp4000425045004750500025050075010001250AmpRSpCas9 Scaffold

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    5372