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  • 0 feature results
pET-MBP-DidCas7-11-His
  • Sequence Map
    Editing disabled on read-only sequence.
    GTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGCAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCBsmBIEsp3IlacI20406080100120140160
    AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAGCCATAGCAGCATAGGGTGATGGCTCTACAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGBbsIHpaIlacI180200220240260280300320340
    TCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTlacI360380400420440460480500520
    ATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACTACGCTGGTCTACGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGBbsIMluIlacI540560580600620640660680700
    GCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACCGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGApaLIlacI720740760780800820840860
    TGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTACAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAABstAPIlacI8809009209409609801,0001,0201,040
    TCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGAGTGTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGGGTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACEcoNIPflMIlacI promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATCACGTACGTTCCTCTACCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGAGTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGGTCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTANaeISphINgoMIV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GCGTCCGGCGTAGAGGATCGAGATCGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGagcAAAACTGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGCAGGCCGCATCTCCTAGCTCTAGCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGGTACtcgTTTTGACTTCTTCCATTTGACCATTAGACCTAATTGCCBarIClaIXbaIBspDIEE2GK4LV6IW8IN10G1544-2677EE2GK4LV6IW8IN10G1544-2647M1S2K1T2M1S2MBPNcoI homologylac operatorRBST7 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    CGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTGCTATTTCCGATATTGCCAGAGCGACTTCAGCCATTCTTTAAGCTCTTTCTATGGCCTTAATTTCAGTGGCAACTCGTAGGCCTATTTGACCTTCTCTTTAAGGGTGTCCAACGCCGTTGACCGCTACCGGGACTGTAATAGAAGACCCGTGTGCTGGCGAAACCACCGATGCGABsaID12KG14YN16GL18AE20VG22KK24FE26KD28TG30IK32VT34VE36HP38DK40LE42EK44FP46QV48AA50TG52DG54PD56II58FW60AH62DR64FG66GY68A1544-2677D12KG14YN16GL18AE20VG22KK24FE26KD28TG30IK32VT34VE36HP38DK40LE42EK44FP46QV48AA50TG52DG54PD56II58FW60AH62DR64FG66GY68A1544-2647MBP1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGGTTAGACCGGACAACCGACTTTAGTGGGGCCTGTTTCGCAAGGTCCTGTTCGACATAGGCAAATGGACCCTACGGCATGCAATGTTGCCGTTCGACTAACGAATGGGCTAGCGACAACTTCGCAATAGCGACTAAATATTGTTTCTAGACGACGGCTTGGGCGGTTTTTGGACCCAloIPsiIBglIIBsiWIQ70SG72LL74AE76IT78PD80KA82FQ84DK86LY88PF90TW92DA94VR96YN98GK100LI102AY104PI106AV108EA110LS112LI114YN116KD118LL120PN122PP124KT126W1544-2677Q70SG72LL74AE76IT78PD80KA82FQ84DK86LY88PF90TW92DA94VR96YN98GK100LI102AY104PI106AV108EA110LS112LI114YN116KD118LL120PN122PP124KT126W1544-2647MBP1,7601,7801,8001,8201,8401,8601,8801,9001,920
    AAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCTTCTCTAGGGCCGCGACCTATTTCTTGACTTTCGCTTTCCATTCTCGCGCGACTACAAGTTGGACGTTCTTGGCATGAAGTGGACCGGCGACTAACGACGACTGCCCCCAATACGCAAGTTCATACTTTTGCCGTTCATGCTGTAATTTCTGCACCCGCACCTATTGCGACCGCGBmgBIE128EI130PA132LD134KE136LK138AK140GK142SA144LM146FN148LQ150EP152YF154TW156PL158IA160AD162GG164YA166FK168YE170NG172KY174DI176KD178VG180VD182NA184GA1861544-2677E128EI130PA132LD134KE136LK138AK140GK142SA144LM146FN148LQ150EP152YF154TW156PL158IA160AD162GG164YA166FK168YE170NG172KY174DI176KD178VG180VD182NA184GA1861544-2647MBP1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTTTCGCCCAGACTGGAAGGACCAACTGGACTAATTTTTGTTTGTGTACTTACGTCTGTGGCTAATGAGGTAGCGTCTTCGACGGAAATTATTTCCGCTTTGTCGCTACTGGTAGTTGCCGGGCACCCGTACCAGGTTGTAGCTGTGGTCGTTTCACTTAATACCACATTGCCATBstAPIKA188GL190TF192LV194DL196IK198NK200HM202NA204DT206DY208SI210AE212AA214FN216KG218ET220AM222TI224NG226PW228AW230SN232ID234TS236KV238NY240GV242TV2441544-2677KA188GL190TF192LV194DL196IK198NK200HM202NA204DT206DY208SI210AE212AA214FN216KG218ET220AM222TI224NG226PW228AW230SN232ID234TS236KV238NY240GV242TV2441544-2647MBP2,1202,1402,1602,1802,2002,2202,2402,260
    CTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAGACGGCTGGAAGTTCCCAGTTGGTAGGTTTGGCAAGCAACCGCACGACTCGCGTCCATAATTGCGGCGGTCAGGCTTGTTTCTCGACCGTTTTCTCAAGGAGCTTTTGATAGACGACTGACTACTTCCAGACCTTCGCCAATTATTTCTGTTTGGCGACCCACGGCATCGCGACTLP246TF248KG250QP252SK254PF256VG258VL260SA262GI264NA266AS268PN270KE272LA274KE276FL278EN280YL282LT284DE286GL288EA290VN292KD294KP296LG298AV300AL3021544-2677LP246TF248KG250QP252SK254PF256VG258VL260SA262GI264NA266AS268PN270KE272LA274KE276FL278EN280YL282LT284DE286GL288EA290VN292KD294KP296LG298AV300AL3021544-2647MBP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGATCAGAATGCTCCTTCTCAACCGCTTTCTAGGTGCATAACGGCGGTGATACCTTTTGCGGGTCTTTCCACTTTAGTACGGCTTGTAGGGCGTCTACAGGCGAAAGACCATACGGCACGCATGACGCCACTAGTTGCGGCGGTCGCCAGCAGTCTGACAGCTACTTCGGGACTTTCTKS304YE306EE308LA310KD312PR314IA316AT318ME320NA322QK324GE326IM328PN330IP332QM334SA336FW338YA340VR342TA344VI346NA348AS350GR352QT354VD356EA358LK360D1544-2677KS304YE306EE308LA310KD312PR314IA316AT318ME320NA322QK324GE326IM328PN330IP332QM334SA336FW338YA340VR342TA344VI346NA348AS350GR352QT354VD356EA358LK360D1544-2647MBP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGAGGGAAGGATTTCAGAATTCCTGGAAGTTCTGTTCCAGGGGCCCGGATCCATGACCACTACCATGAAGATCAGCATCGAGTTCTTGGAGCCCTTCCGCATGACGAAGTGGCAGGCGCGTCTGATTAAGCTCGAGCTTGTTGTTGTTGTTATTGTTATTGTTGTTGGAGCCCTAGCTCCCTTCCTAAAGTCTTAAGGACCTTCAAGACAAGGTCCCCGGGCCTAGGTACTGGTGATGGTACTTCTAGTCGTAGCTCAAGAACCTCGGGAAGGCGTACTGCTTCACCGTCAloISacIBamHIEcoRIEco53kIA362QT364NS366SS368NN370NN372NN374NN376NN3781544-2677F1L2E3V4L5F6Q7G8P9G10S112705-2737A362QT364NS366SS3681544-2647L1G2I3E4G5R6I7S8E9F10L11E12V13L14F15Q16G17P18G19S202678-2737MT2TT4MK6IS8IE10FL12EP14FR16MT18KW20Q2738-7540DidCas7-11HRV3CMBP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GAGTCCACGCGCCGCAATAAGAACAATAAAGAGTTTGTGCGTGGCCAAGCGTTTGCCCGTTGGCACCGCAACAAGAAAGATAACACCAAGGGTCGTCCGTACATTACTGGTACGCTCCTGCGCTCAGCGGTGATTCGCTCCGCGgagAACCTGCTGACACTGAGTGACGGTAAGACTCAGGTGCGCGGCGTTATTCTTGTTATTTCTCAAACACGCACCGGTTCGCAAACGGGCAACCGTGGCGTTGTTCTTTCTATTGTGGTTCCCAGCAGGCATGTAATGACCATGCGAGGACGCGAGTCGCCACTAAGCGAGGCGCctcTTGGACGACTGTGACTCACTGCCATTCTMscIBglIIDraIIIE22ST24RR26NK28NN30KE32FV34RG36QA38FA40RW42HR44NK46KD48NT50KG52RP54YI56TG58TL60LR62SA64VI66RS68AE70NL72LT74LS76DG78K2738-7540DidCas7-112,8202,8402,8602,8802,9002,9202,9402,960
    TCTCCGAGAAAACCTGCTGCCCGGGGAAATTCGACACGGAAGATAAGGACCGTTTACTCCAGCTGCGTCAACGCTCCACGCTGCGTTGGACAGACAAGAATCCGTGTCCCGACAATGCGGAAACGTATTGTCCGTTCTGTGAGCTCCTCGGCCGCTCCGGAAATGACGGTAAGAAAGAGGCTCTTTTGGACGACGGGCCCCTTTAAGCTGTGCCTTCTATTCCTGGCAAATGAGGTCGACGCAGTTGCGAGGTGCGACGCAACCTGTCTGTTCTTAGGCACAGGGCTGTTACGCCTTTGCATAACAGGCAAGACACTCGAGGAGCCGGCGAGGCCTTTACTGCCATTCTTPsrISacISmaIXmaITspMIEco53kII80SE82KT84CC86PG88KF90DT92ED94KD96RL98LQ100LR102QR104ST106LR108WT110DK112NP114CP116DN118AE120TY122CP124FC126EL128LG130RS132GN134DG136KK1382738-7540DidCas7-112,9803,0003,0203,0403,0603,0803,1003,1203,140
    AGCAGAAAAGAAGGACTGGCGCTTCCGCATCCATTTTGGTAACCTTTCCCTGCCGGGCAAGCCCGACTTCGACGGTCCGAAGGCAATTGGTTCACAGCGCGTATTAAATCGTGTGGATTTTAAGTCGGGAAAGGCACATGACTTCTTCAAGGCTTATGAGGTGGACCATACCCGCTCGTCTTTTCTTCCTGACCGCGAAGGCGTAGGTAAAACCATTGGAAAGGGACGGCCCGTTCGGGCTGAAGCTGCCAGGCTTCCGTTAACCAAGTGTCGCGCATAATTTAGCACACCTAAAATTCAGCCCTTTCCGTGTACTGAAGAAGTTCCGAATACTCCACCTGGTATGGGCGMfeIRsrIIAE140KK142DW144RF146RI148HF150GN152LS154LP156GK158PD160FD162GP164KA166IG168SQ170RV172LN174RV176DF178KS180GK182AH184DF186FK188AY190EV192DH194TR1962738-7540DidCas7-113,1603,1803,2003,2203,2403,2603,2803,3003,320
    TTCCCTCGCTTTGAAGGGGAGATCACCATCGACAATAAAGTGTCCGCCGAGGCACGTAAGTTGCTGTGTGACAGCCTTAAATTCACGGATCGCCTGTGTGGAGCCCTGTGCGTCATCCGCTTCGACGAGTATACACCGGCCGCGGACAGCGGTAAGCAAACCGAGAATGTGCAGGAAGGGAGCGAAACTTCCCCTCTAGTGGTAGCTGTTATTTCACAGGCGGCTCCGTGCATTCAACGACACACTGTCGGAATTTAAGTGCCTAGCGGACACACCTCGGGACACGCAGTAGGCGAAGCTGCTCATATGTGGCCGGCGCCTGTCGCCATTCGTTTGGCTCTTACACGTCCBstZ17IFP198RF200EG202EI204TI206DN208KV210SA212EA214RK216LL218CD220SL222KF224TD226RL228CG230AL232CV234IR236FD238EY240TP242AA244DS246GK248QT250EN252VQ2542738-7540DidCas7-113,3403,3603,3803,4003,4203,4403,4603,4803,500
    CAGAGCCCAACGCAAACCTGGCGGAGAAAACTGCCGAGCAAATTATCTCGATCCTCGATGACAACAAGAAAACGGAATACACCCGCCTTTTAGCGGACGCCATCCGCTCATTGCGTCGCTCCTCAAAACTTGTTGCAGGTTTGCCCAAGGATCACGATGGTAAGGACGATCATTAGTCTCGGGTTGCGTTTGGACCGCCTCTTTTGACGGCTCGTTTAATAGAGCTAGGAGCTACTGTTGTTCTTTTGCCTTATGTGGGCGGAAAATCGCCTGCGGTAGGCGAGTAACGCAGCGAGGAGTTTTGAACAACGTCCAAACGGGTTCCTAGTGCTACCATTCCTGCTAGTAATAE256PN258AN260LA262EK264TA266EQ268II270SI272LD274DN276KK278TE280YT282RL284LA286DA288IR290SL292RR294SS296KL298VA300GL302PK304DH306DG308KD310DH312Y2738-7540DidCas7-113,5203,5403,5603,5803,6003,6203,6403,660
    CTTGtggGACATTGGGAAGAAGAAGAAGGATGAGAATTCGGTTACCATCCGTCAGATTCTTACCACGAGTGCCGACACGAAGGAGCTGAAGAACGCTGGTAAGTGGCGCGAGTTTTGTGAGAAGCTTGGTGAGGCGCTTTATTTGAAGAGTAAGGATATGAGCGGCGGGTTGAAGGAACaccCTGTAACCCTTCTTCTTCTTCCTACTCTTAAGCCAATGGTAGGCAGTCTAAGAATGGTGCTCACGGCTGTGCTTCCTCGACTTCTTGCGACCATTCACCGCGCTCAAAACACTCTTCGAACCACTCCGCGAAATAAACTTCTCATTCCTATACTCGCCGCCCAACTTCBssSIEcoRIBssSαIL314WD316IG318KK320KK322DE324NS326VT328IR330QI332LT334TS336AD338TK340EL342KN344AG346KW348RE350FC352EK354LG356EA358LY360LK362SK364DM366SG368GL370K2738-7540DidCas7-113,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATTACACGCCGTATTCTCGGGGACGCAGAGTTTCATGGCAAGCCAGATCGCTTGGAAAAGTCCCGTAGTGTCTCGATCGGAAGCGTGTTAAAGGAAACCGTGGTGTGCGGAGAACTCGTCGCAAAGACCCCGTTCTTCTTTGGCGCGATCGACGAGGATGCAAAACAAACTgccTTAATGTGCGGCATAAGAGCCCCTGCGTCTCAAAGTACCGTTCGGTCTAGCGAACCTTTTCAGGGCATCACAGAGCTAGCCTTCGCACAATTTCCTTTGGCACCACACGCCTCTTGAGCAGCGTTTCTGGGGCAAGAAGAAACCGCGCTAGCTGCTCCTACGTTTTGTTTGAcggAI372TR374RI376LG378DA380EF382HG384KP386DR388LE390KS392RS394VS396IG398SV400LK402ET404VV406CG408EL410VA412KT414PF416FF418GA420ID422ED424AK426QT428A2738-7540DidCas7-113,8603,8803,9003,9203,9403,9603,9804,0004,020
    TACAGGTACTGCTTACTCCGGACAATAAGTATCGCCTGCCGCGCAGCGCCGTCCGCGGCATCCTTCGCCGCGATCTTCAAACGTACTTTGACAGTCCCTGTAATGCGGAGTTAGGCGGTCGTCCATGTATGTGCAAGACCTGTCGTATCATGCGTGGCATTACGGTTATGgacGCATGTCCATGACGAATGAGGCCTGTTATTCATAGCGGACGGCGCGTCGCGGCAGGCGCCGTAGGAAGCGGCGCTAGAAGTTTGCATGAAACTGTCAGGGACATTACGCCTCAATCCGCCAGCAGGTACATACACGTTCTGGACAGCATAGTACGCACCGTAATGCCAATACctgCGL430QV432LL434TP436DN438KY440RL442PR444SA446VR448GI450LR452RD454LQ456TY458FD460SP462CN464AE466LG468GR470PC472MC474KT476CR478IM480RG482IT484VM486DA4882738-7540DidCas7-114,0404,0604,0804,1004,1204,1404,1604,1804,200
    GCGCTCCGAGTATAACGCGCCCCCGGAGATTCGTCACCGTACCCGCATTAATCCGTTTACGGGAACAGTTGCTGAGGGCGCCCTGTTTAATATGGAAGTGGCCCCGGAAGGGATCGTATTCCCATTCCAATTACGCTATCGCGGCTCTGAGGACGGGCTCCCAGACGCGCTCAAGCGCGAGGCTCATATTGCGCGGGGGCCTCTAAGCAGTGGCATGGGCGTAATTAGGCAAATGCCCTTGTCAACGACTCCCGCGGGACAAATTATACCTTCACCGGGGCCTTCCCTAGCATAAGGGTAAGGTTAATGCGATAGCGCCGAGACTCCTGCCCGAGGGTCTGCGCGAGTTCBbvCIBpu10IRS490EY492NA494PP496EI498RH500RT502RI504NP506FT508GT510VA512EG514AL516FN518ME520VA522PE524GI526VF528PF530QL532RY534RG536SE538DG540LP542DA544LK5462738-7540DidCas7-114,2204,2404,2604,2804,3004,3204,3404,360
    ACCGTGCTGAAGTGGTGGGCAGAGGGGCAAGCTTTTATGTCAGGGGCCGCCAGTACCGGGAAAGGCCGTTTTCGTATGGAGAATGCCAAATATGAAACCTTGGATCTGAGCGATGAGAATCAACGCAATGACTACTTGAAGAATTGGGGCTGGCGTGACGAGAAGGGTCTTGAGGTGGCACGACTTCACCACCCGTCTCCCCGTTCGAAAATACAGTCCCCGGCGGTCATGGCCCTTTCCGGCAAAAGCATACCTCTTACGGTTTATACTTTGGAACCTAGACTCGCTACTCTTAGTTGCGTTACTGATGAACTTCTTAACCCCGACCGCACTGCTCTTCCCAGAACTCCTV548LK550WW552AE554GQ556AF558MS560GA562AS564TG566KG568RF570RM572EN574AK576YE578TL580DL582SD584EN586QR588ND590YL592KN594WG596WR598DE600KG602LE6042738-7540DidCas7-114,3804,4004,4204,4404,4604,4804,5004,5204,540
    AGCTTAAGAAACGTTTAAATTCAGGTCTGCCGGAGCCTGGCAATTACCGTGACCCGAAGTGGCATGAAATCAACGTGTCAATCGAGATGGCGAGTCCCTTCATCAACGGCgacCCTATCCGCGCAGCTGTAGACAAGCGTGGGACGgccGTCGTGACCTTCGTTAAGTACAAGGCTCGAATTCTTTGCAAATTTAAGTCCAGACGGCCTCGGACCGTTAATGGCACTGGGCTTCACCGTACTTTAGTTGCACAGTTAGCTCTACCGCTCAGGGAAGTAGTTGCCGctgGGATAGGCGCGTCGACATCTGTTCGCACCCTGCcggCAGCACTGGAAGCAATTCATGTTCCGDraIAflIIPshAIEL606KK608RL610NS612GL614PE616PG618NY620RD622PK624WH626EI628NV630SI632EM634AS636PF638IN640GD642PI644RA646AV648DK650RG652TA654VV656TF658VK660YK662A2738-7540DidCas7-114,5604,5804,6004,6204,6404,6604,6804,7004,720
    GGAAGGGGAAGAAGCAAAGCCCGTGTGCGCATATAAGGCGGAGTCGTTTCGCGGCGTCATTCGTTCGGCTGTAGCACGCATTCACATGGAAGATGGAGTGCCCCTGACTGAGCTTACTCACTCAGATTGCGAGTGTTTGCTCTGTCAAATCTTCGGGTCGGAATATGAGGCTGGTCCTTCCCCTTCTTCGTTTCGGGCACACGCGTATATTCCGCCTCAGCAAAGCGCCGCAGTAAGCAAGCCGACATCGTGCGTAAGTGTACCTTCTACCTCACGGGGACTGACTCGAATGAGTGAGTCTAACGCTCACAAACGAGACAGTTTAGAAGCCCAGCCTTATACTCCGACCAFspIFspAIE664GE666EA668KP670VC672AY674KA676ES678FR680GV682IR684SA686VA688RI690HM692ED694GV696PL698TE700LT702HS704DC706EC708LL710CQ712IF714GS716EY718EA720G2738-7540DidCas7-114,7404,7604,7804,8004,8204,8404,8604,8804,900
    AAGATTCGTTTCGAAgatTTAGTATTTGAGAGTGACCCGGAGCCGGTCACCTTCGATCACGTTGCAATCgacCGCTTCACCGGCGGTGCCGCTGACAAGAAGAAGTTCGACGACAGTCCTTTGCCAGGGTCGCCGGCGCGCCCTCTGATGCTGAAGGGTTCCTTCTGGATTCGCCTTCTAAGCAAAGCTTctaAATCATAAACTCTCACTGGGCCTCGGCCAGTGGAAGCTAGTGCAACGTTAGctgGCGAAGTGGCCGCCACGGCGACTGTTCTTCTTCAAGCTGCTGTCAGGAAACGGTCCCAGCGGCCGCGCGGGAGACTACGACTTCCCAAGGAAGACCTAAGCGGAscIMreINaeIEcoNIPshAINgoMIVK722IR724FE726DL728VF730ES732DP734EP736VT738FD740HV742AI744DR746FT748GG750AA752DK754KK756FD758DS760PL762PG764SP766AR768PL770ML772KG774SF776WI778R2738-7540DidCas7-114,9204,9404,9604,9805,0005,0205,0405,060
    GTGACGTTCTCGAAGATGAGGAGTATTGCAAAGCGCTCGGGAAAGCATTAGCCGATGTAAATAATGGTTTATATCCTCTGGGTGGAAAATCCGCCATCGGATACGGACAAGTTAAGAGCCTGGGTATCAAGGGCGACGATAAGCGTATCAGCCGTTTAATGAATCCGGCCTTCGACACTGCAAGAGCTTCTACTCCTCATAACGTTTCGCGAGCCCTTTCGTAATCGGCTACATTTATTACCAAATATAGGAGACCCACCTTTTAGGCGGTAGCCTATGCCTGTTCAATTCTCGGACCCATAGTTCCCGCTGCTATTCGCATAGTCGGCAAATTACTTAGGCCGGAAGCTR780DV782LE784DE786EY788CK790AL792GK794AL796AD798VN800NG802LY804PL806GG808KS810AI812GY814GQ816VK818SL820GI822KG824DD826KR828IS830RL832MN834PA836FD8382738-7540DidCas7-115,0805,1005,1205,1405,1605,1805,2005,2205,240
    CGAAACCGACGTGGCGGTTCCAGAGAAGCCTAAAACCGATGCCGAGGTCCGTATCGAAGCTGAGAAGGTATATTATCCCCACTACTTTGTGGAACCGCACAAGAAGGTAGAGCGCGAGGAGAAGCCGTGCGGTCATCAAAAGTTTCATGAGGGACGCCTTACCGGTAAGATTCGTGCTTTGGCTGCACCGCCAAGGTCTCTTCGGATTTTGGCTACGGCTCCAGGCATAGCTTCGACTCTTCCATATAATAGGGGTGATGAAACACCTTGGCGTGTTCTTCCATCTCGCGCTCCTCTTCGGCACGCCAGTAGTTTTCAAAGTACTCCCTGCGGAATGGCCATTCTAAGCAAgeIBmgBIET840DV842AV844PE846KP848KT850DA852EV854RI856EA858EK860VY862YP864HY866FV868EP870HK872KV874ER876EE878KP880CG882HQ884KF886HE888GR890LT892GK894IR8962738-7540DidCas7-115,2605,2805,3005,3205,3405,3605,3805,4005,420
    TGTAAACTTATTACGAAAACGCCTCTCATTGTGCCGGACACCAGCAATGATGACTTCTTTCGCCCTGCGGACAAAGAGGCCCGCAAGGAGAAGGACGAGTATCACAAATCCTATGCCTTCTTTCGCCTGCATAAGCAAATCATGATCCCCGGTAGTGAACTCCGCGGGATGGTGTACATTTGAATAATGCTTTTGCGGAGAGTAACACGGCCTGTGGTCGTTACTACTGAAGAAAGCGGGACGCCTGTTTCTCCGGGCGTTCCTCTTCCTGCTCATAGTGTTTAGGATACGGAAGAAAGCGGACGTATTCGTTTAGTACTAGGGGCCATCACTTGAGGCGCCCTACCACACK898LI900TK902TP904LI906VP908DT910SN912DD914FF916RP918AD920KE922AR924KE926KD928EY930HK932SY934AF936FR938LH940KQ942IM944IP946GS948EL950RG952MV9542738-7540DidCas7-115,4405,4605,4805,5005,5205,5405,5605,5805,600
    CCTCTGTTTACGAAACGGTTACAAATTCATGTTTCCGTATCTTTGACGAGACTAAGCGTCTTAGTTGGCGCATGGATGCCGATCACCAAAACGTTTTACAAGATTTCTTACCGGGCCGTGTTACGGCCGACGGCAAGCACATTCAAAAGTTTTCCGAGACTGCACGTGTACCGTTGGAGACAAATGCTTTGCCAATGTTTAAGTACAAAGGCATAGAAACTGCTCTGATTCGCAGAATCAACCGCGTACCTACGGCTAGTGGTTTTGCAAAATGTTCTAAAGAATGGCCCGGCACAATGCCGGCTGCCGTTCGTGTAAGTTTTCAAAAGGCTCTGACGTGCACATGGCAAAhdIPmlISS956VY958ET960VT962NS964CF966RI968FD970ET972KR974LS976WR978MD980AD982HQ984NV986LQ988DF990LP992GR994VT996AD998GK1000HI1002QK1004FS1006ET1008AR1010VP1012F2738-7540DidCas7-115,6205,6405,6605,6805,7005,7205,7405,760
    TTATgacAAAACGCAAAAGCACTTTGACATTCTCGACGAGCAGGAAATTGCCGGTGAAAAGCCGGTCCGCATGTGGGTGAAGCGTTTCATTAAGCGCCTGAGCCTGGTGGACCCTGCAAAGCACCCACAAAAGAAGCAAGACAATAAGTGGAAACGCCGCAAGGAAGGTATTGCGAATActgTTTTGCGTTTTCGTGAAACTGTAAGAGCTGCTCGTCCTTTAACGGCCACTTTTCGGCCAGGCGTACACCCACTTCGCAAAGTAATTCGCGGACTCGGACCACCTGGGACGTTTCGTGGGTGTTTTCTTCGTTCTGTTATTCACCTTTGCGGCGTTCCTTCCATAACGCRsrIIBpu10IY1014DK1016TQ1018KH1020FD1022IL1024DE1026QE1028IA1030GE1032KP1034VR1036MW1038VK1040RF1042IK1044RL1046SL1048VD1050PA1052KH1054PQ1056KK1058QD1060NK1062WK1064RR1066KE1068GI1070A2738-7540DidCas7-115,7805,8005,8205,8405,8605,8805,9005,9205,940
    ACGTTCATTGAGCAGAAGAATGGGTCTTACTATTTTAACGTGGTCACCAACAATGGCTGCACCAGCTTTCACTTGTGGCACAAGCCAGACAACTTCGACCAAGAGAAGTTAGAGGGCATCCAAAACGGGGAGAAGTTAGATTGCTGGGTTCGTGATAGCCGTTACCAAAAGGCATTGCAAGTAACTCGTCTTCTTACCCAGAATGATAAAATTGCACCAGTGGTTGTTACCGACGTGGTCGAAAGTGAACACCGTGTTCGGTCTGTTGAAGCTGGTTCTCTTCAATCTCCCGTAGGTTTTGCCCCTCTTCAATCTAACGACCCAAGCACTATCGGCAATGGTTTTCCGTAT1072FI1074EQ1076KN1078GS1080YY1082FN1084VV1086TN1088NG1090CT1092SF1094HL1096WH1098KP1100DN1102FD1104QE1106KL1108EG1110IQ1112NG1114EK1116LD1118CW1120VR1122DS1124RY1126QK1128A2738-7540DidCas7-115,9605,9806,0006,0206,0406,0606,0806,1006,120
    TTCAAGAAATCCCGGAGAACGATCCGGACGGATGGGAGTGTAAGGAAGGTTATCTGCATGTGGTGGGCCCTTCGAAGGTTGAATTTAGCGACAAGAAGGGCGATGTCATTAATAACTTCCAAGGGACTCTCCCATCAGTGCCCAATGATTGGAAAACGATTCGTACAAACGACTTAAGTTCTTTAGGGCCTCTTGCTAGGCCTGCCTACCCTCACATTCCTTCCAATAGACGTACACCACCCGGGAAGCTTCCAACTTAAATCGCTGTTCTTCCCGCTACAGTAATTATTGAAGGTTCCCTGAGAGGGTAGTCACGGGTTACTAACCTTTTGCTAAGCATGTTTGCTGAAAjuIPflMIF1130QE1132IP1134EN1136DP1138DG1140WE1142CK1144EG1146YL1148HV1150VG1152PS1154KV1156EF1158SD1160KK1162GD1164VI1166NN1168FQ1170GT1172LP1174SV1176PN1178DW1180KT1182IR1184TN1186DF11882738-7540DidCas7-116,1406,1606,1806,2006,2206,2406,2606,2806,300
    TAAGAACCGCAAACGTAAGAACGAGCCCGTTTTCTGTTGCGAGGATGACAAGGGGAACTACTACACGATGGCCAAATATTGTGAAACGTTCTTCTTCGACCTGAAAGAGAACGAGGAGTATGAGATCCCCGAGAAGGCACGTATCAAGTACAAGGAGTTATTACGCGTGTACAACATTCTTGGCGTTTGCATTCTTGCTCGGGCAAAAGACAACGCTCCTACTGTTCCCCTTGATGATGTGCTACCGGTTTATAACACTTTGCAAGAAGAAGCTGGACTTTCTCTTGCTCCTCATACTCTAGGGGCTCTTCCGTGCATAGTTCATGTTCCTCAATAATGCGCACATGTTGMluIMscISspIBsrGIKN1190RK1192RK1194NE1196PV1198FC1200CE1202DD1204KG1206NY1208YT1210MA1212KY1214CE1216TF1218FF1220DL1222KE1224NE1226EY1228EI1230PE1232KA1234RI1236KY1238KE1240LL1242RV1244YN12462738-7540DidCas7-116,3206,3406,3606,3806,4006,4206,4406,460
    AACAATCCGCAAGCGGTTCCCGAGAGCGTGTTCCAGAGTCGCGTAGCGCGCGAGAACGTAGAAAAGCTGAAGTCTGGGGATTTGGTGTATTTCAAGCACAACGAGAAATATGTTGAGGACATCGTACCGGTTCGTATTTCACGTACCGTAGACGACCGCATGATCGGAAAGCGTATTGTTAGGCGTTCGCCAAGGGCTCTCGCACAAGGTCTCAGCGCATCGCGCGCTCTTGCATCTTTTCGACTTCAGACCCCTAAACCACATAAAGTTCGTGTTGCTCTTTATACAACTCCTGTAGCATGGCCAAGCATAAAGTGCATGGCATCTGCTGGCGTACTAGCCTTTCGCATAgeINN1248PQ1250AV1252PE1254SV1256FQ1258SR1260VA1262RE1264NV1266EK1268LK1270SG1272DL1274VY1276FK1278HN1280EK1282YV1284ED1286IV1288PV1290RI1292SR1294TV1296DD1298RM1300IG1302KR13042738-7540DidCas7-116,4806,5006,5206,5406,5606,5806,6006,6206,640
    TGTCGGCTGATTTGCGTCCATGTCACGGTGACTGGGTAGAGGACGGGGACCTCTCTGCGTTGAATGCATATCCAGAGAAGCGCCTTTTATTGCGTCACCCTAAGGGCCTTTGCCCGGCATGTCGCCTTTTCGGAACGGGTAGCTACAAGGGCCGCGTTCGTTTTGGCTTCGCGAGACAGCCGACTAAACGCAGGTACAGTGCCACTGACCCATCTCCTGCCCCTGGAGAGACGCAACTTACGTATAGGTCTCTTCGCGGAAAATAACGCAGTGGGATTCCCGGAAACGGGCCGTACAGCGGAAAAGCCTTGCCCATCGATGTTCCCGGCGCAAGCAAAACCGAAGCGCTCNruINsiIPpuMIBsu36IMS1306AD1308LR1310PC1312HG1314DW1316VE1318DG1320DL1322SA1324LN1326AY1328PE1330KR1332LL1334LR1336HP1338KG1340LC1342PA1344CR1346LF1348GT1350GS1352YK1354GR1356VR1358FG1360FA1362S2738-7540DidCas7-116,6606,6806,7006,7206,7406,7606,7806,8006,820
    CCTGGAGAACGACCCGGAGTGGTTGATTCCTGGAAAGAATCCCGGTGATCCATTCCACGGCGGGCCAGTGATGCTGAGCTTGCTGGAGCGCCCGCGTCCCACCTGGAGCATTCCTGGCAGTGATAATAAGTTCAAAGTTCCAGGCCGTAAGTTCTACGTTCACCATCATGCTTGGGGACCTCTTGCTGGGCCTCACCAACTAAGGACCTTTCTTAGGGCCACTAGGTAAGGTGCCGCCCGGTCACTACGACTCGAACGACCTCGCGGGCGCAGGGTGGACCTCGTAAGGACCGTCACTATTATTCAAGTTTCAAGGTCCGGCATTCAAGATGCAAGTGGTAGTACGAACCL1364EN1366DP1368EW1370LI1372PG1374KN1376PG1378DP1380FH1382GG1384PV1386ML1388SL1390LE1392RP1394RP1396TW1398SI1400PG1402SD1404NK1406FK1408VP1410GR1412KF1414YV1416HH1418HA1420W2738-7540DidCas7-116,8406,8606,8806,9006,9206,9406,9606,9807,000
    AAAACGATTAAGGACGGTAACCATCCGACCACCGGCAAAGCCATTGAACAATCGCCAAATAACCGCACGGTTGAAGCGCTCGCCGGTGGCAACAGCTTCAGCTTCGAAATTGCGTTCGAAAATTTGAAGGAGTGGGAGTTGGGCCTGCTGATCCATAGCCTGCAGCTTGAAAAGGTTTTGCTAATTCCTGCCATTGGTAGGCTGGTGGCCGTTTCGGTAACTTGTTAGCGGTTTATTGGCGTGCCAACTTCGCGAGCGGCCACCGTTGTCGAAGTCGAAGCTTTAACGCAAGCTTTTAAACTTCCTCACCCTCAACCCGGACGACTAGGTATCGGACGTCGAACTTTTCCPstIK1422TI1424KD1426GN1428HP1430TT1432GK1434AI1436EQ1438SP1440NN1442RT1444VE1446AL1448AG1450GN1452SF1454SF1456EI1458AF1460EN1462LK1464EW1466EL1468GL1470LI1472HS1474LQ1476LE1478K2738-7540DidCas7-117,0207,0407,0607,0807,1007,1207,1407,160
    GCTTGGCCCATAAGCTCGGTATGGCTAAGAGCATGGGCTTTGGCTCAGTAGAGATCGACGTTGAGAGTGTCCGCCTGCGTAAAGATTGGAAGCAGTGGCGCAATGGTAATTCAGAGATCCCAAATTGGTTGGGCAAGGGGTTTGCAAAGCTGAAGGAGTGGTTCCGCGACGAGCTCGAACCGGGTATTCGAGCCATACCGATTCTCGTACCCGAAACCGAGTCATCTCTAGCTGCAACTCTCACAGGCGGACGCATTTCTAACCTTCGTCACCGCGTTACCATTAAGTCTCTAGGGTTTAACCAACCCGTTCCCCAAACGTTTCGACTTCCTCACCAAGGCGCTGCTCGAG1480LA1482HK1484LG1486MA1488KS1490MG1492FG1494SV1496EI1498DV1500ES1502VR1504LR1506KD1508WK1510QW1512RN1514GN1516SE1518IP1520NW1522LG1524KG1526FA1528KL1530KE1532WF1534RD1536EL15382738-7540DidCas7-117,1807,2007,2207,2407,2607,2807,3007,3207,340
    GGACTTCATCGAGAATTTGAAGAAATTACTGTGGTTCCCCGAAGGTGATCAAGCTCCGCGCGTATGTTACCCCATGCTTCGCAAGAAGGATGACCCTAATGGAAACTCAGGTTATGAGGAGTTGAAAGACGGCGAATTTAAGAAGGAAGATCGTCAGAAGAAGCTTACAACACCACCTGAAGTAGCTCTTAAACTTCTTTAATGACACCAAGGGGCTTCCACTAGTTCGAGGCGCGCATACAATGGGGTACGAAGCGTTCTTCCTACTGGGATTACCTTTGAGTCCAATACTCCTCAACTTTCTGCCGCTTAAATTCTTCCTTCTAGCAGTCTTCTTCGAATGTTGTGGTNcoIDF1540IE1542NL1544KK1546LL1548WF1550PE1552GD1554QA1556PR1558VC1560YP1562ML1564RK1566KD1568DP1570NG1572NS1574GY1576EE1578LK1580DG1582EF1584KK1586ED1588RQ1590KK1592LT1594TP15962738-7540DidCas7-117,3607,3807,4007,4207,4407,4607,4807,5007,520
    TGGACGCCGTGGGCTAGCAGCGGCCTGGTGCCGCGCGGCAGCCATCATCATCACCACCATCACTAATTAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGACCTGCGGCACCCGATCGTCGCCGGACCACGGCGCGCCGTCGGTAGTAGTAGTGGTGGTAGTGATTAATTGGATCCGACGACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTAACCBmtINheIAvrIIS1S2G3L4V5P6R7G8S9H10H11H12H13H14H15H16*177541-7591WT1598PW1600A2738-7540DidCas7-117,5407,5607,5807,6007,6207,6407,6607,6807,700
    CGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGC7,7207,7407,7607,7807,8007,820
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7825 bp5500600065007000750050010001500DidCas7-11MBPATGlacI1544-26777541-75911544-26472738-7540Kaz-pMal-no-linker-r (DNA)Kaz-pMal-SSS-linker-r (DNA)Kaz-MBP-tga-inf-r (DNA)PU_22_pMal-HRV3C-inf-r (DNA)BsmBI,Esp3I,HpaI,BbsIBbsIMluIApaLIBstAPIlacI promoter,PflMI,EcoNISphIT7 promoter,NgoMIV,NaeI,BspDI,ClaIlac operator,NcoI homology,RBS,RBS,1532-1537,+7BsaIKaz-SER1-M-r (DNA),Kaz-SER1-M-f (DNA)AloI,BsiWIPsiI,BglIIBpu10IAjuIPflMIMscI,SspIMluI,BsrGIAgeIPpuMI,NsiIBsu36INruIPstIPU_73_Kaz-pET-last-f (DNA),Kaz-CHis-inf-pACYC-siteB-r (DNA),NcoI,NheI,BmtI,AvrII

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    7825