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XZ066 (PB)EF1a-LIDARst(CCR6V2, IRESv0)-T2A-PuroR-P2A-BFP
  • Sequence Map
    Editing disabled on read-only sequence.
    tgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccagAhdIT286*K284T*282GY280NG278SE276GD274HL272YS270RY268PL266A*264WR262AG260TS258CH256YR254SV252WA250*R248SW246I*244*C242YV240LW238GS236PG234LA232ST230T1-12308: Region 1Amp20406080100120140160
    ctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtgacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaR228CS226*G224GG222DL220RN218IT216AP214FS212SY210TT208RW206NI204TQ202AV200NN198GN196SC194AD192HH190*A188RR186KT184HS182*E180AG178TG176LS174*P172SN1701-12308: Region 1Amp180200220240260280300320340
    tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcScaICS168GG166HQ164AF162FR160NA158GE156TR154RD152ND150ST148LQ146GC144H*142*E140HN138HC136CQ134MI132RK130SD128HW126GY124SA122KR120HS118TL116V*114GL1121-12308: Region 1Amp360380400420440460480500520
    tcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgag*E110SF108LT106HP104SR102TA100RA98RR96*Y94PI92IG90RW88MA86SS84*F82HE80DN78SF76TR74RP72SF70E*68PD66*R64QQ62SG60TR58HL56GS541-12308: Region 1Amp540560580600620640660680700
    gtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcTC52GV50SR48*C46RK44SE42GA40NR38PS36CF34CS32PL30IG28CF26LS24YP22RC20PF18TS16YE14D1-12308: Region 1Amp720740760780800820840860
    cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttatagcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatPsiICspCI8809009209409609801,0001,0201,040
    aatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcg1,0601,0801,1001,1201,1401,1601,1801,2001,220
    actaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtc1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcctccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgcgga1,4201,4401,4601,4801,5001,5201,5401,560
    cgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtgacgtacgttaaagataatcatgtgtaaaattggcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacactgcatgcaatttctattagtacacattttaacBsiWI5' PB ITR Long5' PB ITR shortjt10091,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacaaaaactcaaaatttcttctataaagtaacaaaatgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtttttgagttttaaagaagatatttcattgtttt5' PB ITR Long1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcggaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccctgtcgggcccgtgccccttccaccgtgccctagcSrfIRsrIIcore insul1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATCCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTAGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATSfiISpeIattD Cp36core insulForward Targettarget ...primer2,1202,1402,1602,1802,2002,2202,2402,260
    GGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGMfeIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1a2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAASacIIEF-1α promoter2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAAflIIEF-1α promoter2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTABglIISacIIEF-1α promoter2,8202,8402,8602,8802,9002,9202,9402,960
    GTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCFseIEF-1α promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCBbvCIEF-1α promoterfrag13,1603,1803,2003,2203,2403,2603,2803,3003,320
    TCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGEF-1α promoterfrag1RF from EF-1a3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CATTTCAGGTGTCGTGAATCTAGAAGCCACCatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatATGAGCGGAGAGAGCATGAACTTCAGTGATGTGTTCGATTCCAGTGAGGACTACTTCGTGTCCGTAAAGTCCACAGCACTTAGATCTTCGGTGGtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctaTACTCGCCTCTCTCGTACTTGAAGTCACTACACAAGCTAAGGTCACTCCTGATGAAGCACAGGClaIXbaIBspDIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28SG30ES32MN34FS36DV38FD40SS42ED44YF46VS483532-6045Signal/FlagCCR6EF-1α...oterfrag1RF fr...F-1ahomolog3,5203,5403,5603,5803,6003,6203,6403,660
    GTCAATACATCCTACTACAGCGTAGATTCTGAGATGCTGCTTTGCTCCCTGCAGGAGGTGCGCCAGTTCTCCCGACTCTTCGTTCCCATCGCGTATTCACTCATCTGCGTCTTTGGCCTCCTGGGGAATATCTTGGTAGTCATCACATTTGCTTTTTATAAAAAGGCCAGATCCACAGTTATGTAGGATGATGTCGCATCTAAGACTCTACGACGAAACGAGGGACGTCCTCCACGCGGTCAAGAGGGCTGAGAAGCAAGGGTAGCGCATAAGTGAGTAGACGCAGAAACCGGAGGACCCCTTATAGAACCATCAGTAGTGTAAACGAAAAATATTTTTCCGGTCTAGGTPsiISbfIVN50TS52YY54SV56DS58EM60LL62CS64LQ66EV68RQ70FS72RL74FV76PI78AY80SL82IC84VF86GL88LG90NI92LV94VI96TF98AF100YK102KA104RS1063532-6045CCR63,6803,7003,7203,7403,7603,7803,8003,8203,840
    TGACAGACGTCTACCTGCTTAATATGGCCATTGCAGACATCCTGTTCGTCCTGACGCTCCCATTCTGGGCTGTGAGTCACGCTACCGGGGCCTGGGTGTTCAGTAATGCAACCTGCAAGCTGTTGAAGGGAATTTATGCCATCAACTTTAATTGCGGTATGCTGCTGCTGACTTGACTGTCTGCAGATGGACGAATTATACCGGTAACGTCTGTAGGACAAGCAGGACTGCGAGGGTAAGACCCGACACTCAGTGCGATGGCCCCGGACCCACAAGTCATTACGTTGGACGTTCGACAACTTCCCTTAAATACGGTAGTTGAAATTAACGCCATACGACGACGACTGAACMT108DV110YL112LN114MA116IA118DI120LF122VL124TL126PF128WA130VS132HA134TG136AW138VF140SN142AT144CK146LL148KG150IY152AI154NF156NC158GM160LL162LT164C3532-6045CCR63,8603,8803,9003,9203,9403,9603,9804,0004,020
    CATAAGTATGGACAGATACATCGCCATAGTACAGGCAACAAAATCCTTCAGACTGAGGTCTCGAACGCTCCCTAGGTCTAAAATCATTTGTCTTGTTGTATGGGGCTTGAGCGTGATTATTTCCAGCAGTACTTTCGTGTTTAACCAGAAGTACAACACGCAGGGCAGCGATGTGGTATTCATACCTGTCTATGTAGCGGTATCATGTCCGTTGTTTTAGGAAGTCTGACTCCAGAGCTTGCGAGGGATCCAGATTTTAGTAAACAGAACAACATACCCCGAACTCGCACTAATAAAGGTCGTCATGAAAGCACAAATTGGTCTTCATGTTGTGCGTCCCGTCGCTACACScaIAvrIII166SM168DR170YI172AI174VQ176AT178KS180FR182LR184SR186TL188PR190SK192II194CL196VV198WG200LS202VI204IS206SS208TF210VF212NQ214KY216NT218QG220SD222V3532-6045CCR64,0404,0604,0804,1004,1204,1404,1604,1804,200
    TGTGAGCCCAAGTACCAGACGGTATCTGAGCCTATCAGGTGGAAGCTGTTGATGCTGGGTTTGGAATTGCTGTTCGGCTTTTTTATCCCACTCATGTTTATGATTTTTTGTTATACCTTCATCGTGAAGACCCTGGTCCAGGCACAAAACTCTAAGCGACACAAGGCCATCCGGGACACTCGGGTTCATGGTCTGCCATAGACTCGGATAGTCCACCTTCGACAACTACGACCCAAACCTTAACGACAAGCCGAAAAAATAGGGTGAGTACAAATACTAAAAAACAATATGGAAGTAGCACTTCTGGGACCAGGTCCGTGTTTTGAGATTCGCTGTGTTCCGGTAGGCCCC224EP226KY228QT230VS232EP234IR236WK238LL240ML242GL244EL246LF248GF250FI252PL254MF256MI258FC260YT262FI264VK266TL268VQ270AQ272NS274KR276HK278AI280R3532-6045CCR64,2204,2404,2604,2804,3004,3204,3404,360
    TAATCATCGCTGTTGTGCTGGTATTTCTGGCTTGCCAGATCCCTCATAATATGGTCCTTCTGGTAACGGCAGCCAACCTGGGCAAAATGAATCGCTCATGTCAGTCAGAAAAGCTTATCGGGTACACCAAAACGGTTACGGAGGTTCTGGCGTTCCTGCATTGTTGCCTTAACCCATTAGTAGCGACAACACGACCATAAAGACCGAACGGTCTAGGGAGTATTATACCAGGAAGACCATTGCCGTCGGTTGGACCCGTTTTACTTAGCGAGTACAGTCAGTCTTTTCGAATAGCCCATGTGGTTTTGCCAATGCCTCCAAGACCGCAAGGACGTAACAACGGAATTGGGV282II284AV286VL288VF290LA292CQ294IP296HN298MV300LL302VT304AA306NL308GK310MN312RS314CQ316SE318KL320IG322YT324KT326VT328EV330LA332FL334HC336CL338NP3403532-6045CCR64,3804,4004,4204,4404,4604,4804,5004,5204,540
    TGTGCTGTACGCATTTATAGGTCAGAAGTTTCGAAACTACTTCCTGAAGATTCTCAAAGACCTCTGGTGTGTAAGAAGAAAGTATAAAAGTAGTGGATTTTCATGCGCAGGACGCTACTCCGAAAACATTTCCAGACAGACCAGTGAGACGGCCGATAACGATAACGCCTCCTCCACACGACATGCGTAAATATCCAGTCTTCAAAGCTTTGATGAAGGACTTCTAAGAGTTTCTGGAGACCACACATTCTTCTTTCATATTTTCATCACCTAAAAGTACGCGTCCTGCGATGAGGCTTTTGTAAAGGTCTGTCTGGTCACTCTGCCGGCTATTGCTATTGCGGAGGAGGEagIBstBIVL342YA344FI346GQ348KF350RN352YF354LK356IL358KD360LW362CV364RR366KY368KS370SG372FS374CA376GR378YS380EN382IS384RQ386TS388ET390AD392ND394NA396SS3983532-6045CCR64,5604,5804,6004,6204,6404,6604,6804,7004,720
    TTTACCATGaccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAAATGGTACtggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGKflIKpnIXcmIAcc65IFT400MT402GG404RT406PP408SL410GP412QD414ES416CT418TA420SS422SL424AK426DT428SS430GT432GA434LH436LD438QT440PS442RQ444PI446PS448EG450LQ452LH454LP4563532-6045V2 tailhADAR2 cat domainCCR64,7404,7604,7804,8004,8204,8404,8604,8804,900
    AGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTQV458LA460DA462VS464RL466VL468GK470FG472DL474TD476NF478SS480PH482AR484RK486VL488AG490VV492MT494TG496TD498VK500DA502KV504IS506VS508TG510TK512CI514N3532-6045hADAR2 cat domain4,9204,9404,9604,9805,0005,0205,0405,060
    TGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCG516EY518MS520DR522GL524AL526ND528CH530AE532II534SR536RS538LL540RF542LY544TQ546LE548LY550LN552NK554DD556QK558RS560IF562QK564SE566RG568GF570RL572K3532-6045hADAR2 cat domain5,0805,1005,1205,1405,1605,1805,2005,2205,240
    GAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCACTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTE574NV576QF578HL580YI582ST584SP586CG588DA590RI592FS594PH596EP598IL600EE602PA604DR606HP608NR610KA612RG614QL616RT618KI620ES622GQ624GT626IP628VR630S3532-6045hADAR2 cat domain5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGATACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTBlpIBamHIN632AS634IQ636AW638DG640VL642QG644ER646LL648TM650SC652SD654KI656AR658WN660VV662GI664QG666SL668LS670IF672VE674PI676YF678SS680II682LG684SL686YH688GD6903532-6045hADAR2 cat domainhomolog5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGHincIIHL692SR694AM696YQ698RI700SN702IE704DL706PP708LY710TL712NK714PL716LS718GI720SN722AE724AR726QP728GK730AP732NF734SV736NW738TV740GD742SA744IE746VI7483532-6045hADAR2 cat domain5,6205,6405,6605,6805,7005,7205,7405,760
    AACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCMluINA750TT752GK754DE756LG758RA760SR762LC764KH766AL768YC770RW772MR774VH776GK778VP780SH782LL784RS786KI788TK790PN792VY794HE796SK798LA800AK802EY804QA8063532-6045hADAR2 cat domain5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCTGATAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGACTATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAAK808AR810LF812TA814FI816KA818GL820GA822WV824EK826PT828EQ830DQ832FS834LT836P*8383532-6045hADAR2 cat domainEMCV IRES SitePCRhomolog5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACAAvrIIEMCV IRES Site6,1406,1606,1806,2006,2206,2406,2606,2806,300
    AGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGTCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACPmlIEMCV IRES Site6,3206,3406,3606,3806,4006,4206,4406,460
    AAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGggggagaaacccTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATACCGGTGTTGGTACcccctctttgggKpnIBstXIPflMIAcc65IMG2EK4P6636-8603EMCV IRES SiteARRB26,4806,5006,5206,5406,5606,5806,6006,6206,640
    gggaccagggtcttcaagaagtcgagccctaactgcaagctcaccgtgtacttgggcaagcgggacttcgtagatcacctggacaaagtggaccctgtagatggcgtggtgcttgtggaccctgactacctgaaggaccgcaaagtgtttgtgaccctcacctgcgccttccgctccctggtcccagaagttcttcagctcgggattgacgttcgagtggcacatgaacccgttcgccctgaagcatctagtggacctgtttcacctgggacatctaccgcaccacgaacacctgggactgatggacttcctggcgtttcacaaacactgggagtggacgcggaaggcgaG6TR8VF10KK12SS14PN16CK18LT20VY22LG24KR26DF28VD30HL32DK34VD36PV38DG40VV42LV44DP46DY48LK50DR52KV54FV56TL58TC60AF62R6636-8603ARRB2homolog6,6606,6806,7006,7206,7406,7606,7806,8006,820
    atggccgtgaagacctggatgtgctgggcttgtccttccgcaaagacctgttcatcgccacctaccaggccttccccccggtgcccaacccaccccggccccccacccgcctgcaggaccggctgctgaggaagctgggccagcatgcccaccccttcttcttcaccataccccataccggcacttctggacctacacgacccgaacaggaaggcgtttctggacaagtagcggtggatggtccggaaggggggccacgggttgggtggggccggggggtgggcggacgtcctggccgacgactccttcgacccggtcgtacgggtggggaagaagaagtggtatggggtSbfIBbvCIY64GR66ED68LD70VL72GL74SF76RK78DL80FI82AT84YQ86AF88PP90VP92NP94PR96PP98TR100LQ102DR104LL106RK108LG110QH112AH114PF116FF118TI120PQ1226636-8603ARRB26,8406,8606,8806,9006,9206,9406,9606,9807,000
    gaatcttccatgctccgtcacactgcagccaggcccagaggatacaggaaaggcctgcggcgtagactttgagattcgagccttctgtgctaaatcactagaagagaaaagccacaaaaggaactctgtgcggctggtgatccgaaaggtgcagttcgccccggagaaacccggccttagaaggtacgaggcagtgtgacgtcggtccgggtctcctatgtcctttccggacgccgcatctgaaactctaagctcggaagacacgatttagtgatcttctcttttcggtgttttccttgagacacgccgaccactaggctttccacgtcaagcggggcctctttgggccgNL124PC126SV128TL130QP132GP134ED136TG138KA140CG142VD144FE146IR148AF150CA152KS154LE156EK158SH160KR162NS164VR166LV168IR170KV172QF174AP176EK178PG1806636-8603ARRB27,0207,0407,0607,0807,1007,1207,1407,160
    ccccagccttcagccgaaaccacacgccacttcctcatgtctgaccggtccctgcacctcgaggcttccctggacaaggagctgtactaccatggggagcccctcaatgtaaatgtccacgtcaccaacaactccaccaagaccgtcaagaagatcaaagtctctgtgagacagtggggtcggaagtcggctttggtgtgcggtgaaggagtacagactggccagggacgtggagctccgaagggacctgttcctcgacatgatggtacccctcggggagttacatttacaggtgcagtggttgttgaggtggttctggcagttcttctagtttcagagacactctgtcaPspXIPQ182PS184AE186TT188RH190FL192MS194DR196SL198HL200EA202SL204DK206EL208YY210HG212EP214LN216VN218VH220VT222NN224ST226KT228VK230KI232KV234SV236RQ2386636-8603ARRB27,1807,2007,2207,2407,2607,2807,3007,3207,340
    acgccgacatctgcctcttcagcaccgcccagtacaagtgtcctgtggctcaactcgaacaagatgaccaggtatctcccagctccacattctgtaaggtgtacaccataaccccactgctcagcgacaaccgggagaagcggggtctcgccctggatgggaaactcaagcacgatgcggctgtagacggagaagtcgtggcgggtcatgttcacaggacaccgagttgagcttgttctactggtccatagagggtcgaggtgtaagacattccacatgtggtattggggtgacgagtcgctgttggccctcttcgccccagagcgggacctaccctttgagttcgtgctBlpISexAIYA240DI242CL244FS246TA248QY250KC252PV254AQ256LE258QD260DQ262VS264PS266ST268FC270KV272YT274IT276PL278LS280DN282RE284KR286GL288AL290DG292KL294KH296E6636-8603ARRB27,3607,3807,4007,4207,4407,4607,4807,5007,520
    ggacaccaacctggcttccagcaccatcgtgaaggagggtgccaacaaggaggtgctgggaatcctggtgtcctacagggtcaaggtgaagctggtggtgtctcgaggcggggatgtctctgtggagctgccttttgttcttatgcaccccaagccccacgaccacatccccctccctgtggttggaccgaaggtcgtggtagcacttcctcccacggttgttcctccacgacccttaggaccacaggatgtcccagttccacttcgaccaccacagagctccgcccctacagagacacctcgacggaaaacaagaatacgtggggttcggggtgctggtgtagggggagAleID298TN300LA302SS304TI306VK308EG310AN312KE314VL316GI318LV320SY322RV324KV326KL328VV330SR332GG334DV336SV338EL340PF342VL344MH346PK348PH350DH352IP354L6636-8603ARRB27,5407,5607,5807,6007,6207,6407,6607,6807,700
    cccagaccccagtcagccgctccggagacagatgtccctgtggacaccaacctcattgaatttgataccaactatgccacagatgatgacattgtgtttgaggactttgcccggcttcggctgaaggggatgaaggatgacgactatgatgatcaactctgcggcGGTGGTAGCGgggtctggggtcagtcggcgaggcctctgtctacagggacacctgtggttggagtaacttaaactatggttgatacggtgtctactactgtaacacaaactcctgaaacgggccgaagccgacttcccctacttcctactgctgatactactagttgagacgccgCCACCATCGCAfeIP356RP358QS360AA362PE364TD366VP368VD370TN372LI374EF376DT378NY380AT382DD384DI386VF388ED390FA392RL394RL396KG398MK400DD402DY404DD406QL408CG410GG412S6636-8603ARRB27,7207,7407,7607,7807,8007,8207,8407,860
    CTATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAGATACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTHincIIA414MA416SN418FT420QF422VL424VD426NG428GT430GD432VT434VA436PS438NF440AN442GI444AE446WI448SS450NS452RS454QA456YK458VT460CS462VR464QS466SA468QN470RK4726636-8603MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPhomolog7,8807,9007,9207,9407,9607,9808,0008,0208,040
    ATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATXcmIAsiSIBsaBIYT474IK476VE478VP480KG482AW484RS486YL488NM490EL492TI494PI496FA498TN500SD502CE504LI506VK508AM510QG512LL514KD516GN518PI520PS522AI524AA526NS528GI5306636-8603MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP8,0608,0808,1008,1208,1408,1608,1808,2008,220
    TATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGYG532GG534TG536GG538SM540AS542NF544TQ546FV548LV550DN552GG554TG556DV558TV560AP562SN564FA566NG568IA570EW572IS574SN576SR578SQ580AY582KV584TC586SV5886636-8603from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP8,2408,2608,2808,3008,3208,3408,3608,3808,400
    GCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGBstXIBstZ17IRQ590SS592AQ594NR596KY598TI600KV602EV604PK606GA608WR610SY612LN614ME616LT618IP620IF622AT624NS626DC628EL630IV632KA634MQ636GL638LK640DG642NP644IP646S6636-8603from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP8,4208,4408,4608,4808,5008,5208,5408,560
    CGCAATCGCTGCGAATAGCGGAATTTACGCTAGCGAAGGTCGGGGGAGTTTGCTTACCTGTGGCGATGTCGAGGAGAATCCAGGGCCCAAGCTTATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCGTTAGCGACGCTTATCGCCTTAAATGCGATCGCTTCCAGCCCCCTCAAACGAATGGACACCGCTACAGCTCCTCTTAGGTCCCGGGTTCGAATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGBmtINheIBsiWIA648IA650AN652SG654IY6566636-8603AS2EG4RG6SL8LT10CG12DV14EE16NP18GP20KL22MT24EY26KP28TV30RL32AT34RD36DV38PR40AV42RT44LA46AA48F8604-10043PuroRfrag3T2Afrom pCDH 3...ozak_tdMCPMCP8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCRsrIIA50DY52PA54TR56HT58VD60PD62RH64IE66RV68TE70LQ72EL74FL76TR78VG80LD82IG84KV86WV88AD90DG92AA94VA96VW98TT100PE102SV104EA106G8604-10043PuroRfrag3PR_S030PR_S0298,7608,7808,8008,8208,8408,8608,8808,9008,920
    CGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCAA108VF110AE112IG114PR116MA118EL120SG122SR124LA126AQ128QQ130ME132GL134LA136PH138RP140KE142PA144WF146LA148TV150GV152SP154DH156QG158KG160LG162SA164VV1668604-10043PuroRfrag38,9408,9608,9809,0009,0209,0409,0609,0809,100
    GCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACCGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGEagISexAILP168GV170EA172AE174RA176GV178PA180FL182ET184SA186PR188NL190PF192YE194RL196GF198TV200TA202DV204EV206PE208GP210RT212WC214MT216RK218PG220AA222TN2248604-10043frag3PuroRP2A9,1209,1409,1609,1809,2009,2209,2409,260
    TTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgaggAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctccSapIBamHIBspQIFS226LL228KQ230AG232DV234EE236NP238GP240GS242MV244SK246GE248EL250IK252EN254MH256MK258LY260ME262GT264VD266NH268HF270KC272TS274EG276EG278KP280YE2828604-10043frag4mtagBFP2frag3P2Ahomologoushomolog9,2809,3009,3209,3409,3609,3809,4009,4209,440
    gcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccaccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgBsu36IGT284QT286MR288IK290VV292EG294GP296LP298FA300FD302IL304AT306SF308LY310GS312KT314FI316NH318TQ320GI322PD324FF326KQ328SF330PE332GF334TW336ER338VT340T8604-10043frag4mtagBFP29,4609,4809,5009,5209,5409,5609,5809,6009,620
    atacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggctatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccgY342ED344GG346VL348TA350TQ352DT354SL356QD358GC360LI362YN364VK366IR368GV370NF372TS374NG376PV378MQ380KK382TL384GW386EA388FT390ET392LY394PA396DG398G8604-10043frag4mtagBFP29,6409,6609,6809,7009,7209,7409,7609,7809,800
    ctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagcL400EG402RN404DM406AL408KL410VG412GS414HL416IA418NA420KT422TY424RS426KK428PA430KN432LK434MP436GV438YY440VD442YR444LE446RI448KE450AN452NE454TY456V8604-10043frag4mtagBFP29,8209,8409,8609,8809,9009,9209,9409,960
    agcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAGAATTCCTCGAGCTAGAGCTcGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCtcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTCTTAAGGAGCTCGATCTCGAgCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGPacIAlwNIEcoRIPspXIE458QH460EV462AV464AR466YC468DL470PS472KL474GH476KL478N*4808604-10043bGH poly(A) signalfrag4mtagBFP2homolog9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatctataacaagaaTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagatattgttcttEcoRVbGH poly(A) signal3' PB...long10,16010,18010,20010,22010,24010,26010,28010,30010,320
    aatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctc3' PB ITR long10,34010,36010,38010,40010,42010,44010,46010,48010,500
    ctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgccgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcggNsiI3' PB ITR long3' PB ITR shortjt101010,52010,54010,56010,58010,60010,62010,64010,660
    caagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgagttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccact10,68010,70010,72010,74010,76010,78010,80010,82010,840
    actgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgagtgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctc10,86010,88010,90010,92010,94010,96010,98011,00011,020
    gacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctactgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggat11,04011,06011,08011,10011,12011,14011,16011,18011,200
    atgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgtactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacSapIBspQI11,22011,24011,26011,28011,30011,32011,34011,360
    cggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttag11,38011,40011,42011,44011,46011,48011,50011,52011,540
    gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccataga11,56011,58011,60011,62011,64011,66011,68011,70011,720
    cagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcgtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgAlwNI11,74011,76011,78011,80011,82011,84011,86011,88011,900
    ggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattaccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaat11,92011,94011,96011,98012,00012,02012,04012,060
    cgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatact12,08012,10012,12012,14012,16012,18012,20012,22012,240
    gtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatccatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagL12A*10DT8LC6RD4*R2D1-12308: Region 2Amp12,26012,28012,300
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12308 bp90001000011000100020003000homologPrimer 11-12308: Region 11-12308: Region 28604-10043XZp032.3 (DNA)oAPL0043 (DNA)XZp050.3 (DNA)XZp053.4 (DNA)XZp050.4 (DNA)oLSM0403 (DNA)oLSM0248 (DNA)XZp044.2 (DNA)XZp044.4 (DNA)XZp044.5 (DNA)Amp,AhdIScaICspCIPsiIjt1009BsiWI5' PB ITR Long,5' PB ITR shortcore insul,RsrII,SrfIForward Target,target forward primer,SfiI,SpeIattD Cp36Reverse TargetTarget Reverse Primer,primer from EF-1a,MfeISacIIAflIIEF-1α promoter,BglII,SacIIFseIP2A,homologous,XZp044.3 (DNA),SexAI,BamHIBspQISapImTagBFP2.1 (DNA),Bsu36Ifrag4mtagBFP2homolog,AlwNI,PacI,EcoRI,PspXIbGH poly(A) signalEcoRV3' PB ITR long3' PB ITR short,NsiIjt1010BspQISapIAlwNI

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    12308