Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV-EF1a-double floxed opto A1-EYFP-WPRE
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaagctttgcaaagatggataggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcgaaacgtttctacctatMluISbfIHindIIIL-ITREF-1a20406080100120140160
    aagttttaaacagagaggaatctttgcagctaatggaccttctaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaattcaaaatttgtctctccttagaaacgtcgattacctggaagatccagaactttcctcacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttggccacggatctcttccaccgcgccccattAgeIMfeIEcoNIEF-1a180200220240260280300320340
    actgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggccctgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccattcacggcacacaccaagggcgcccggaccggagaaatgcccaataccgggEF-1a360380400420440460480500520
    ttgcgtgccttgaattacttccactggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccaacgcacggaacttaatgaaggtgaccgacgtcatgcactaagaactagggctcgaagcccaaccttcacccaccctctcaagctccggaacgcgaattcctcggggaagcggagcacgaactcaactccggaccggacccgcgaccccggcggcgcacgcttagaccaccgtggStuIStuIAflIIEF-1a540560580600620640660680700
    ttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacaagcgcggacagagcgacgaaagctattcagagatcggtaaattttaaaaactactggacgacgctgcgaaaaaaagaccgttctatcagaacatttacgcccggttctagacgtgtgaccataaagccaaaaaccccggcgcccgccgctgccccgggcacgcagggtcgcgtgApaIPciIBfuAIBspMIPspOMIEF-1a720740760780800820840860
    atgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttccctacaagccgctccgccccggacgctcgcgccggtggctcttagcctgcccccatcagagttcgaccggccggacgagaccacggaccggagcgcggcggcacatagcggggcgggacccgccgttccgaccgggccagccgtggtcaacgcactcgcctttctaccggcgaagggFseIBstAPIEF-1a8809009209409609801,0001,0201,040
    ggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtccgggacgacgtccctcgagttttacctcctgcgccgcgagccctctcgcccgcccactcagtgggtgtgtttccttttcccggaaaggcaggagtcggcagcgaagtacactgaggtgcctcatggcccgcggcaggtccgtggagctaatcaagagctcgaaaacctcatgcaKasINarISacISfoITliIXhoIBbvCIEcoNIPluTIPaeR7IEco53kIEF-1a1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttctgcagaaatccaacccccctccccaaaatacgctacctcaaaggggtgtgactcacccacctctgacttcaatccggtcgaaccgtgaactacattaagaggaaccttaaacgggaaaaactcaaacctagaaccaagtaagagttcggagtctgtcaccaagtttcaaaaaaagaDraIIIEF-1a1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tccatttcaggtgtcgtgaggtaccggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccaggtaaagtccacagcactccatggcctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcggAscIKpnISalIXbaIBamHIBspEIAcc65IHincIIlox2722loxPEF-1a1,4201,4401,4601,4801,5001,5201,5401,560
    ctattacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctgggataatgaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcccgcctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccBsrGIEYFP (no start)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctatcaccagccgctcgacgtgcgacggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagctacgggaagtcgaEYFP (no start)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagcactgcaggccgtagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgtgacgtccggcatEYFP (no start)1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gccgaaggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaaccggcttccaccagtgctcccacccggtcccgtgcccgtcgaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtggtggggccacttgEYFP (no start)2,1202,1402,1602,1802,2002,2202,2402,260
    agctcctcgcccttgctcacggcggccgcggcgggggcaacctgtgaagtttcaacttcctcgccgttttcgctcaaggatatagtgtgcaccttgatggtgggaacctgatggttcttatcgagggagggggttgaggggccaacacagcagcagacctgaagaaaggatttggtcgaggagcgggaacgagtgccgccggcgccgcccccgttggacacttcaaagttgaaggagcggcaaaagcgagttcctatatcacacgtggaactaccacccttggactaccaagaatagctccctcccccaactccccggttgtgtcgtcgtctggacttctttcctaaaccopto-a1AREYFP (no start)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    agcgcacgcgggctgtggtacagctggactgatccttagaaactgtgatccttgcagatcccctgggcatggaagagaaaaacttccactcgcagactccgtcggtcttactaatccgataaaatgtctccctggaaccgactggtatccgcaccatatccttgtgttggccctctcgcgtgcgcccgacaccatgtcgacctgactaggaatctttgacactaggaacgtctaggggacccgtaccttctctttttgaaggtgagcgtctgaggcagccagaatgattaggctattttacagagggaccttggctgaccataggcgtggtataggaacacaaccgggagPflFITth111Iopto-a1AR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tacagcctgactgggtgggtgcagagtataccccagggcatgcttactagactgcttccgacagaggcattggattctcagcacattctgaaatgccttccggaactgcttgttcatcataatatagataacggggttatacacggcagaggtcttggcgaagaaggcagggatgatgtcggactgacccacccacgtctcatatggggtcccgtacgaatgatctgacgaaggctgtctccgtaacctaagagtcgtgtaagactttacggaaggccttgacgaacaagtagtattatatctattgccccaatatgtgccgtctccagaaccgcttcttccgtccctacBspEIBstZ17Iopto-a1AR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gtcataaagattgggccgaagtcagaaccttgatgtgtgaagatgtaaaacgcgactccagcgtaaggcagccagcaaatgagaaaggcgataaccatgatgatgaccatgcgcgccgcctttttctcgcgggagaacttgagaagccgcacggaaaagtgggttttagtcttggcagtatttctaacccggcttcagtcttggaactacacacttctacattttgcgctgaggtcgcattccgtcggtcgtttactctttccgctattggtactactactggtacgcgcggcggaaaaagagcgccctcttgaactcttcggcgtgccttttcacccaaaatcagaaccopto-a1AR2,8202,8402,8602,8802,9002,9202,9402,960
    cggatgccatcccgcttccgcctgctggggcgttcttcctatgaatgcgaagggtcacctgctcggaatcgctcttatctgtcttcagtccagatttcaagcccctggactcccttttggccacgacgtagacgcgtccatagcaaaaaaaaataacaatcaaggggatgataaagcctacggtagggcgaaggcggacgaccccgcaagaaggatacttacgcttcccagtggacgagccttagcgagaatagacagaagtcaggtctaaagttcggggacctgagggaaaaccggtgctgcatctgcgcaggtatcgtttttttttattgttagttcccctactatttBsmIMluIBfuAIBspMIPaqCIBstEIIopto-a1AR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtggactacaaacatatatatgacgaaggactcgttgtttgtctcttcgtgcggggtatagtagtcgattccgcaagaacactgcataccttctggaatatagcgggaccaacccaccagggggggagcagcacaggccaaagccatgacccaggtaaaggctacacccataatgcacctgatgtttgtatatatactgcttcctgagcaacaaacagagaagcacgccccatatcatcagctaaggcgttcttgtgacgtatggaagaccttatatcgccctggttgggtggtccccccctcgtcgtgtccggtttcggtactgggtccatttccgatgtgggtattacPsrIopto-a1AR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gccctccgctgggtcactatggtgggatatctgagaggataagacaccaccacataccgctcgatggccaggacgaccaggctccaaagagcgatctcaccgccaagagtggcgaagaagccttccaagttgcagcctgtgggcccgaacacaaaataaccatgcagggacgtatcgggaggcgacccagtgataccaccctatagactctcctattctgtggtggtgtatggcgagctaccggtcctgctggtccgaggtttctcgctagagtggcggttctcaccgcttcttcggaaggttcaacgtcggacacccgggcttgtgttttattggtacgtccctgcataApaIEcoRVPspOMIBstZ17Iopto-a1AR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    acagagtagtcgtaaacccgccgaacaccatgaaaagatctgccaccgcgaggttcagaagaatataattcaatacggagtgcaaatgtctgtggcatgctaccacatacagggtaagaaaattgatgggaaaccccagcataatcagaaggaacatataagcggccagcattgatgtctcatcagcatttgggcggcttgtggtacttttctagacggtggcgctccaagtcttcttatattaagttatgcctcacgtttacagacaccgtacgatggtgtatgtcccattcttttaactaccctttggggtcgtattagtcttccttgtatattcgccggtcgtaactopto-a1AR3,5203,5403,5603,5803,6003,6203,6403,660
    gaattgccacggctctgcgaggtagtactgtggggcttcgaatggactgcgcaccacgccagtcttgttactaaagggtacgtaaaaatttggaccctcggtcccattcatggtggctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattacttaacggtgccgagacgctccatcatgacaccccgaagcttacctgacgcgtggtgcggtcagaacaatgatttcccatgcatttttaaacctgggagccagggtaagtaccaccgatcgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatBmtIFspINheIScaIBstBISnaBIopto-a1ARlox27223,6803,7003,7203,7403,7603,7803,8003,8203,840
    tcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaClaIBspDIEcoRIEcoRVHindIIIWPREloxP3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggaPflMIWPRE4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctgcagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacWPRE4,2204,2404,2604,2804,3004,3204,3404,360
    gattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctcAfeIBplIClaITliIXhoIZraIAatIIBspDIPaeR7IWPRE4,3804,4004,4204,4404,4604,4804,5004,5204,540
    agatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcatctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgtBplICspCIhGH polyA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacBlpINsiIBbvCIhGH polyA4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtAloIBsaIPmlIBsaXIBstXIRsrIIBstEIIhGH polyA4,9204,9404,9604,9805,0005,0205,0405,060
    ggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacKasINarISbfISfoIPluTIR-ITR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggf1 Origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttcggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaagAloIBsaXIDraIIIf1 Origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    caaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagacPsiIf1 ...in5,6205,6405,6605,6805,7005,7205,7405,760
    atgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgattacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcacta5,7805,8005,8205,8405,8605,8805,9005,9205,940
    acgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggtgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccZraIAatII5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagattctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtaScaIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    gacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacctgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgPvuIAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgatactgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatAseIFspIAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagattagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctBsaIAmpR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatccttttggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaapUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    tttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgapUC ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatAlwNIpUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    caccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgagtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactpUC ori7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIpUC ori7,7207,7407,7607,7807,800
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7812 bp5500600065007000750050010001500L-ITR,SbfI,MluI,HindIII,MfeI,AgeI,EcoNIEF-1a,StuI,AflII,StuI,BfuAI,BspMIPspOMI,ApaI,PciI,FseI,BstAPI,EcoNIEco53kI,SacI,BbvCI,KasI,NarI,SfoI,PluTI,PaeR7I,TliI,XhoIDraIII,Acc65I,KpnI,BamHI,XbaI,SalI,HincII,BspEIlox2722loxP,AscI,BsrGIEYFP (no start)ZraIAatIIScaIAmpR,PvuI,FspI,AseIBsaIpUC ori,AlwNI,PciI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7812