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pCMV_T7-PE2-Nuclease
  • Sequence Map
    Editing disabled on read-only sequence.
    acgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattMluIAflIIICMV enhancer20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacNdeICMV enhancer180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgCspCISnaBICMV promoterCMV enhancer360380400420440460480500520
    aactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagagatccgcggccgctaatacgactcactatagggagagccgccaccatgaaacggacagccgacggaagcgagttcgagtcattgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgaccaaatcacttggcagtctaggcgatctctaggcgccggcgattatgctgagtgatatccctctcggcggtggtactttgcctgtcggctgccttcgctcaagctcagtNotISacIIMK2RT4AD6GS8EF10ES12665-7018CMV promoterCMV-FT7T7 promoter540560580600620640660680700
    ccaaagaagaagcggaaagtcgacaagaagtacagcatcggcctggacatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgtggtttcttcttcgcctttcagctgttcttcatgtcgtagccggacctgtagccgtggttgagacacccgacccggcactagtggctgctcatgttccacgggtcgttctttaagttccacgacccgttgtggctggccgtgtcgtagttcttcttggactagcctcgggacgacaPK14KK16RK18VD20KK22YS24IG26LD28IG30TN32SV34GW36AV38IT40DE42YK44VP46SK48KF50KV52LG54NT56DR58HS60IK62KN64LI66GA68LL70665-7018Cas9SV40 NLS720740760780800820840860
    tcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataaagctgtcgccgctttgtcggctccggtgggccgacttctcttggcggtcttcttctatgtggtctgccttcttggcctagacgatagacgttctctagaagtcgttgctctaccggttccacctgctgtcgaagaaggtgtctgaccttctcaggaaggaccaccttctcctattBglIIPflMIFD72SG74ET76AE78AT80RL82KR84TA86RR88RY90TR92RK94NR96IC98YL100QE102IF104SN106EM108AK110VD112DS114FF116HR118LE120ES122FL124VE126ED128K665-7018Cas98809009209409609801,0001,0201,040
    gaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgcttcgtgctcgccgtggggtagaagccgttgtagcacctgctccaccggatggtgctcttcatggggtggtagatggtggactctttctttgaccacctgtcgtggctgttccggctggacgccgactagatagaccgggaccgggtgtactagttcaaggccccggtgaaggacK130HE132RH134PI136FG138NI140VD142EV144AY146HE148KY150PT152IY154HL156RK158KL160VD162ST164DK166AD168LR170LI172YL174AL176AH178MI180KF182RG184HF186L665-7018Cas91,0601,0801,1001,1201,1401,1601,1801,2001,220
    atcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgccctagctcccgctggacttggggctgttgtcgctgcacctgttcgacaagtaggtcgaccacgtctggatgttggtcgacaagctccttttggggtagttgcggtcgccgcacctgcggttccggtaggacagacggtctgactcgttctcgtctgccgaccttttagactagcgggBmgBII188EG190DL192NP194DN196SD198VD200KL202FI204QL206VQ208TY210NQ212LF214EE216NP218IN220AS222GV224DA226KA228IL230SA232RL234SK236SR238RL240EN242LI244A665-7018Cas91,2401,2601,2801,3001,3201,3401,3601,3801,400
    agctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtatcgacgggccgctcttcttcttaccggacaagcctttggactaacgggactcggacccggactgggggttgaagttctcgttgaagctggaccggctcctacggtttgacgtcgactcgttcctgtggatgctgctgctggacctgttggacgaccgggtctagccgctggtcatQ246LP248GE250KK252NG254LF256GN258LI260AL262SL264GL266TP268NF270KS272NF274DL276AE278DA280KL282QL284SK286DT288YD290DD292LD294NL296LA298QI300GD302QY304665-7018Cas91,4201,4401,4601,4801,5001,5201,5401,560
    cgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgcggctggacaaagaccggcggttcttggacaggctgcggtaggacgactcgctgtaggactctcacttgtggctctagtggttccggggggactcgcggagatactagttctctatgctgctcgtggtggtcctggactgggacgactttcgagagcacgccgtcgtcgacggaAD306LF308LA310AK312NL314SD316AI318LL320SD322IL324RV326NT328EI330TK332AP334LS336AS338MI340KR342YD344EH346HQ348DL350TL352LK354AL356VR358QQ360LP362665-7018Cas91,5801,6001,6201,6401,6601,6801,7001,7201,740
    gagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcctcttcatgtttctctaaaagaagctggtctcgttcttgccgatgcggccgatgtaactgccgcctcggtcggtccttctcaagatgttcaagtagttcgggtaggaccttttctacctgccgtggctccttgacgagcacttcgacttgtctctcctggacgacgccttcgtcgEK364YK366EI368FF370DQ372SK374NG376YA378GY380ID382GG384AS386QE388EF390YK392FI394KP396IL398EK400MD402GT404EE406LL408VK410LN412RE414DL416LR418KQ420665-7018Cas91,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagcctggaagctgttgccgtcgtagggggtggtctaggtggaccctctcgacgtgcggtaagacgccgccgtccttctaaaaatgggtaaggacttcctgttggcccttttctagctcttctaggactggaaggcgtaggggatgatgcacccgggagaccggtcccctttgtcgtcRT422FD424NG426SI428PH430QI432HL434GE436LH438AI440LR442RQ444ED446FY448PF450LK452DN454RE456KI458EK460IL462TF464RI466PY468YV470GP472LA474RG476NS478R665-7018Cas91,9401,9601,9802,0002,0202,0402,0602,0802,100
    attcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcacctaagcggacctactggtctttctcgctcctttggtagtgggggaccttgaagctccttcaccacctgttcccgcgaaggcgggtctcgaagtagctcgcctactggttgaagctattcttggacgggttgctcttccacgacgggttcgtgtcggacgacatgctcatgaagtggF480AW482MT484RK486SE488ET490IT492PW494NF496EE498VV500DK502GA504SA506QS508FI510ER512MT514NF516DK518NL520PN522EK524VL526PK528HS530LL532YE534YF536T665-7018Cas92,1202,1402,1602,1802,2002,2202,2402,260
    gtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgcacatattgctcgactggtttcactttatgcactggctcccttactctttcgggcggaaggactcgccgctcgtctttttccggtagcacctggacgacaagttctggttggcctttcactggcacttcgtcgactttctcctgatgaagttcttttagctcacgaagctgaggcV538YN540EL542TK544VK546YV548TE550GM552RK554PA556FL558SG560EQ562KK564AI566VD568LL570FK572TN574RK576VT578VK580QL582KE584DY586FK588KI590EC592FD594S665-7018Cas92,2802,3002,3202,3402,3602,3802,4002,4202,440
    tggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctacctttagaggccgcaccttctagccaagttgcggagggacccgtgtatggtgctagacgacttttaatagttcctgttcctgaaggacctgttactccttttgctcctgtaagaccttctatagcacgactgggactgtgacaaactcctgtctctctactagctccttgccgaEcoRVV596EI598SG600VE602DR604FN606AS608LG610TY612HD614LL616KI618IK620DK622DF624LD626NE628EN630ED632IL634ED636IV638LT640LT642LF644ED646RE648MI650EE652RL654665-7018Cas92,4602,4802,5002,5202,5402,5602,5802,6002,620
    gaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcttttggatacgggtggacaagctgctgtttcactacttcgtcgacttcgccgcctctatgtggccgaccccgtccgactcggccttcgactagttgccgtaggccctgttcgtcaggccgttctgttaggacctaaaggacttcaggctgccgaagcggttgtctttgaagtacAhdIKT656YA658HL660FD662DK664VM666KQ668LK670RR672RY674TG676WG678RL680SR682KL684IN686GI688RD690KQ692SG694KT696IL698DF700LK702SD704GF706AN708RN710FM712665-7018Cas92,6402,6602,6802,7002,7202,7402,7602,7802,800
    cagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccgtcgactaggtgctgctgtcggactggaaatttctcctgtaggtctttcgggtccacaggccggtcccgctatcggacgtgctcgtgtaacggttagaccggccgtcggggcggtaattcttcccgtaggacgtctgtcacttccaccacctgctcgagcactttcactacccggAjuISacIEco53kIQL714IH716DD718SL720TF722KE724DI726QK728AQ730VS732GQ734GD736SL738HE740HI742AN744LA746GS748PA750IK752KG754IL756QT758VK760VV762DE764LV766KV768MG770665-7018Cas92,8202,8402,8602,8802,9002,9202,9402,960
    ggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaagctccgtgttcgggctcttgtagcactagctttaccggtctctcttggtctggtgggtcttccctgtcttcttgtcggcgctctcttacttcgcctagcttctcccgtagtttctcgacccgtcggtctaggactttcttgtggggcaccttttgtgggtcgacgtcttgctcttcgaDraIIIRH772KP774EN776IV778IE780MA782RE784NQ786TT788QK790GQ792KN794SR796ER798MK800RI802EE804GI806KE808LG810SQ812IL814KE816HP818VE820NT822QL824QN826EK828L665-7018Cas92,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaaccatggacatgatggacgtcttacccgccctatacatgcacctggtccttgacctgtagttggccgacaggctgatgctacacctggtatagcacggagtctcgaaagacttcctgctgaggtagctgttgttccacgactggtcttcgctgttcttggccccgttctcgctgttgPflMIY830LY832YL834QN836GR838DM840YV842DQ844EL846DI848NR850LS852DY854DV856DH858IV860PQ862SF864LK866DD868SI870DN872KV874LT876RS878DK880NR882GK884SD886N665-7018Cas93,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccggcagatcacacgggaggcttctccagcacttcttctacttcttgatgaccgccgtcgacgacttgcggttcgactaatgggtctctttcaagctgttagactggttccggctctctccgccggactcgcttgacctattccggccgaagtagttctctgtcgaccacctttgggccgtctagtCspCIV888PS890EE892VV894KK896MK898NY900WR902QL904LN906AK908LI910TQ912RK914FD916NL918TK920AE922RG924GL926SE928LD930KA932GF934IK936RQ938LV940ET942RQ944I665-7018Cas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    caaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgagtttcgtgcaccgtgtctaggacctgagggcctacttgtgattcatgctgctcttactgttcgactaggcccttcactttcactagtgggacttcaggttcgaccacaggctaaaggccttcctaaaggtcaaaatgtttcacgcgctctagttgttgatggtggtgcgggtgctPmlIT946KH948VA950QI952LD954SR956MN958TK960YD962EN964DK966LI968RE970VK972VI974TL976KS978KL980VS982DF984RK986DF988QF990YK992VR994EI996NN998YH1000HA1002HD1004665-7018Cas93,5203,5403,5603,5803,6003,6203,6403,660
    cgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaacgcggatggacttgcggcagcacccttggcgggactagtttttcatgggattcgacctttcgctcaagcacatgccgctgatgttccacatgctgcacgccttctactagcggttctcgctcgtcctttagccgttccgatggcggttcatgaagaagatgtcgttgtagtacttgAjuIBmgBIAY1006LN1008AV1010VG1012TA1014LI1016KK1018YP1020KL1022ES1024EF1026VY1028GD1030YK1032VY1034DV1036RK1038MI1040AK1042SE1044QE1046IG1048KA1050TA1052KY1054FF1056YS1058NI1060MN1062665-7018Cas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacagaaaaagttctggctctaatgggaccggttgccgctctaggccttcgccggagactagctctgtttgccgctttggcccctctagcacaccctattcccggccctaaaacggtggcacgcctttcacgactcgtacggggttcacttatagcactttttctggctccacgtctgtcSphIFF1064KT1066EI1068TL1070AN1072GE1074IR1076KR1078PL1080IE1082TN1084GE1086TG1088EI1090VW1092DK1094GR1096DF1098AT1100VR1102KV1104LS1106MP1108QV1110NI1112VK1114KT1116EV1118QT1120665-7018Cas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaacgccgaagtcgtttctcagataggacgggttctccttgtcgctattcgactagcggtctttcttcctgaccctgggattcttcatgccgccgaagctgtcggggtggcaccggataagacacgaccaccaccggtttcaccttttcccgttcaggttctttgacttctcacacttKflIGG1122FS1124KE1126SI1128LP1130KR1132NS1134DK1136LI1138AR1140KK1142DW1144DP1146KK1148YG1150GF1152DS1154PT1156VA1158YS1160VL1162VV1164AK1166VE1168KG1170KS1172KK1174LK1176SV1178K665-7018Cas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    agagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaatctcgacgacccctagtggtagtacctttcttcgtcgaagctcttcttagggtagctgaaagaccttcggttcccgatgtttcttcactttttcctggactagtagttcgacggattcatgagggacaagctcgaccttttgccggccttctcttacgaccggagacggccgcttBsmIXcmIE1180LL1182GI1184TI1186ME1188RS1190SF1192EK1194NP1196ID1198FL1200EA1202KG1204YK1206EV1208KK1210DL1212II1214KL1216PK1218YS1220LF1222EL1224EN1226GR1228KR1230ML1232AS1234AG1236E665-7018Cas94,2204,2404,2604,2804,3004,3204,3404,360
    ctgcagaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccagacgtcttccctttgcttgaccgggacgggaggtttatacacttgaaggacatggaccggtcggtgatactcttcgacttcccgagggggctcctattactcgtctttgtcgacaaacaccttgtcgtgttcgtgatggacctgctctagtagctcgtctagtcgctcaagaggtL1238QK1240GN1242EL1244AL1246PS1248KY1250VN1252FL1254YL1256AS1258HY1260EK1262LK1264GS1266PE1268DN1270EQ1272KQ1274LF1276VE1278QH1280KH1282YL1284DE1286II1288EQ1290IS1292EF1294S665-7018Cas94,3804,4004,4204,4404,4604,4804,5004,5204,540
    agagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtatctctcactaggaccggctgcgattagacctgtttcacgacaggcggatgttgttcgtggccctattcgggtagtctctcgtccggctcttatagtaggtggacaaatgggactggttagaccctcggggacggcggaagttcatgaaactgtggtggtagctggccttctccatK1296RV1298IL1300AD1302AN1304LD1306KV1308LS1310AY1312NK1314HR1316DK1318PI1320RE1322QA1324EN1326II1328HL1330FT1332LT1334NL1336GA1338PA1340AF1342KY1344FD1346TT1348ID1350RK1352RY1354665-7018Cas94,5604,5804,6004,6204,6404,6604,6804,7004,720
    caccagcacgaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggtgactctggaggatctagcggaggatcctctggcagcgagacaccaggaacaagcgagtcagcaacaccagagagcagtggcgtggtcgtgctttctccacgacctgcggtgggactaggtggtctcgtagtggccggacatgctctgtgcctagctggacagagtcgaccctccactgagacctcctagatcgcctcctaggagaccgtcgctctgtggtccttgttcgctcagtcgttgtggtctctcgtcaccgBamHITS1356TK1358EV1360LD1362AT1364LI1366HQ1368SI1370TG1372LY1374ET1376RI1378DL1380SQ1382LG1384GD1386SG1388GS1390SG1392GS1394SG1396SE1398TP1400GT1402SE1404SA1406TP1408ES1410SG1412665-7018Cas9Reverse transcriptase4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggcagcagcggcggcagcagcaccctaaatatagaagatgagtatcggctacatgagacctcaaaagagccagatgtttctctagggtccacatggctgtctgattttcctcaggcctgggcggaaaccgggggcatgggactggcagttcgccaagctcctctgatcatacctcccgtcgtcgccgccgtcgtcgtgggatttatatcttctactcatagccgatgtactctggagttttctcggtctacaaagagatcccaggtgtaccgacagactaaaaggagtccggacccgcctttggcccccgtaccctgaccgtcaagcggttcgaggagactagtatggagBseRIBsu36IGS1414SG1416GS1418ST1420LN1422IE1424DE1426YR1428LH1430ET1432SK1434EP1436DV1438SL1440GS1442TW1444LS1446DF1448PQ1450AW1452AE1454TG1456GM1458GL1460AV1462RQ1464AP1466LI1468IP1470665-7018Reverse transcriptase4,9204,9404,9604,9805,0005,0205,0405,060
    tgaaagcaacctctacccccgtgtccataaaacaataccccatgtcacaagaagccagactggggatcaagccccacatacagagactgttggaccagggaatactggtaccctgccagtccccctggaacacgcccctgctacccgttaagaaaccagggactaatgattatagactttcgttggagatgggggcacaggtattttgttatggggtacagtgttcttcggtctgacccctagttcggggtgtatgtctctgacaacctggtcccttatgaccatgggacggtcagggggaccttgtgcggggacgatgggcaattctttggtccctgattactaatatcKpnIAcc65ILK1472AT1474ST1476PV1478SI1480KQ1482YP1484MS1486QE1488AR1490LG1492IK1494PH1496IQ1498RL1500LD1502QG1504IL1506VP1508CQ1510SP1512WN1514TP1516LL1518PV1520KK1522PG1524TN1526DY1528R665-7018Reverse transcriptase5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gcctgtccaggatctgagagaagtcaacaagcgggtggaggacatccaccccaccgtgcccaacccttacaacctcttgagcgggctcccaccgtcccaccagtggtacactgtgcttgatttaaaggatgcctttttctgcctgagactccaccccaccagtcagcctctcttccggacaggtcctagactctcttcagttgttcgcccacctcctgtaggtggggtggcacgggttgggaatgttggagaactcgcccgagggtggcagggtggtcaccatgtgacacgaactaaatttcctacggaaaaagacggactctgaggtggggtggtcagtcggagagaagP1530VQ1532DL1534RE1536VN1538KR1540VE1542DI1544HP1546TV1548PN1550PY1552NL1554LS1556GL1558PP1560SH1562QW1564YT1566VL1568DL1570KD1572AF1574FC1576LR1578LH1580PT1582SQ1584PL1586F665-7018Reverse transcriptase5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcctttgagtggagagatccagagatgggaatctcaggacaattgacctggaccagactcccacagggtttcaaaaacagtcccaccctgtttaatgaggcactgcacagagacctagcagacttccggatccagcacccagacttgatcctgctacagtacgtggatgacttaccggaaactcacctctctaggtctctacccttagagtcctgttaactggacctggtctgagggtgtcccaaagtttttgtcagggtgggacaaattactccgtgacgtgtctctggatcgtctgaaggcctaggtcgtgggtctgaactaggacgatgtcatgcacctactgaatgMfeIBamHIA1588FE1590WR1592DP1594EM1596GI1598SG1600QL1602TW1604TR1606LP1608QG1610FK1612NS1614PT1616LF1618NE1620AL1622HR1624DL1626AD1628FR1630IQ1632HP1634DL1636IL1638LQ1640YV1642DD1644L665-7018Reverse transcriptase5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgctggccgccacttctgagctagactgccaacaaggtactcgggccctgttacaaaccctagggaacctcgggtatcgggcctcggccaagaaagcccaaatttgccagaaacaggtcaagtatctggggtatcttctaaaagagggtcagagatggctgactgaggccagaaaacgaccggcggtgaagactcgatctgacggttgttccatgagcccgggacaatgtttgggatcccttggagcccatagcccggagccggttctttcgggtttaaacggtctttgtccagttcatagaccccatagaagattttctcccagtctctaccgactgactccggtctttL1646LA1648AT1650SE1652LD1654CQ1656QG1658TR1660AL1662LQ1664TL1666GN1668LG1670YR1672AS1674AK1676KA1678QI1680CQ1682KQ1684VK1686YL1688GY1690LL1692KE1694GQ1696RW1698LT1700EA1702RK1704665-7018Reverse transcriptase5,6205,6405,6605,6805,7005,7205,7405,760
    agagactgtgatggggcagcctactccaaagacccctcgacaactaagggagttcctagggaaggcaggcttctgtcgcctcttcatccctgggtttgcagaaatggcagcccccctgtaccctctcaccaaaccggggactctgtttaattggggcccagaccaacaaaaggcctctctgacactaccccgtcggatgaggtttctggggagctgttgattccctcaaggatcccttccgtccgaagacagcggagaagtagggacccaaacgtctttaccgtcggggggacatgggagagtggtttggcccctgagacaaattaaccccgggtctggttgttttccggET1706VM1708GQ1710PT1712PK1714TP1716RQ1718LR1720EF1722LG1724KA1726GF1728CR1730LF1732IP1734GF1736AE1738MA1740AP1742LY1744PL1746TK1748PG1750TL1752FN1754WG1756PD1758QQ1760KA1762665-7018Reverse transcriptase5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tatcaagaaatcaagcaagcccttctaactgccccagccctggggttgccagatttgactaagccctttgaactctttgtcgacgagaagcagggctacgccaaaggtgtcctaacgcaaaaactgggaccttggcgtcggccggtggcctacctgtccaaaaagctagacccagatagttctttagttcgttcgggaagattgacggggtcgggaccccaacggtctaaactgattcgggaaacttgagaaacagctgctcttcgtcccgatgcggtttccacaggattgcgtttttgaccctggaaccgcagccggccaccggatggacaggtttttcgatctgggtcDrdIYQ1764EI1766KQ1768AL1770LT1772AP1774AL1776GL1778PD1780LT1782KP1784FE1786LF1788VD1790EK1792QG1794YA1796KG1798VL1800TQ1802KL1804GP1806WR1808RP1810VA1812YL1814SK1816KL1818DP1820665-7018Reverse transcriptase5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tagcagctgggtggcccccttgcctacggatggtagcagccattgccgtactgacaaaggatgcaggcaagctaaccatgggacagccactagtcattctggccccccatgcagtagaggcactagtcaaacaaccccccgaccgctggctttccaacgcccggatgactcactaatcgtcgacccaccgggggaacggatgcctaccatcgtcggtaacggcatgactgtttcctacgtccgttcgattggtaccctgtcggtgatcagtaagaccggggggtacgtcatctccgtgatcagtttgttggggggctggcgaccgaaaggttgcgggcctactgagtgatVA1822AG1824WP1826PC1828LR1830MV1832AA1834IA1836VL1838TK1840DA1842GK1844LT1846MG1848QP1850LV1852IL1854AP1856HA1858VE1860AL1862VK1864QP1866PD1868RW1870LS1872NA1874RM1876TH1878Y665-7018Reverse transcriptase6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tcaggccttgcttttggacacggaccgggtccagttcggaccggtggtagccctgaacccggctacgctgctcccactgcctgaggaagggctgcaacacaactgccttgatatcctggccgaagcccacggaacccgacccgacctaacggaccagccgctcccagacgccgacagtccggaacgaaaacctgtgcctggcccaggtcaagcctggccaccatcgggacttgggccgatgcgacgagggtgacggactccttcccgacgttgtgttgacggaactataggaccggcttcgggtgccttgggctgggctggattgcctggtcggcgagggtctgcggctgAgeIEcoRVPflFIRsrIIRsrIIBsu36ITth111IQ1880AL1882LL1884DT1886DR1888VQ1890FG1892PV1894VA1896LN1898PA1900TL1902LP1904LP1906EE1908GL1910QH1912NC1914LD1916IL1918AE1920AH1922GT1924RP1926DL1928TD1930QP1932LP1934DA1936D665-7018Reverse transcriptase6,3206,3406,3606,3806,4006,4206,4406,460
    cacacctggtacacggatggaagcagtctcttacaagagggacagcgtaaggcgggagctgcggtgaccaccgagaccgaggtaatctgggctaaagccctgccagccgggacatccgctcagcgggctgaactgatagcactcacccaggccctaaagatggcagaaggtaagagtgtggaccatgtgcctaccttcgtcagagaatgttctccctgtcgcattccgccctcgacgccactggtggctctggctccattagacccgatttcgggacggtcggccctgtaggcgagtcgcccgacttgactatcgtgagtgggtccgggatttctaccgtcttccattctSexAIBstEIIH1938TW1940YT1942DG1944SS1946LL1948QE1950GQ1952RK1954AG1956AA1958VT1960TE1962TE1964VI1966WA1968KA1970LP1972AG1974TS1976AQ1978RA1980EL1982IA1984LT1986QA1988LK1990MA1992EG1994K665-7018Reverse transcriptase6,4806,5006,5206,5406,5606,5806,6006,6206,640
    agctaaatgtttatactgatagccgttatgcttttgctactgcccatatccatggagaaatatacagaaggcgtgggtggctcacatcagaaggcaaagagatcaaaaataaagacgagatcttggccctactaaaagccctctttctgcccaaaagacttagcataatccattgtcgatttacaaatatgactatcggcaatacgaaaacgatgacgggtataggtacctctttatatgtcttccgcacccaccgagtgtagtcttccgtttctctagtttttatttctgctctagaaccgggatgattttcgggagaaagacgggttttctgaatcgtattaggtaacBglIIK1996LN1998VY2000TD2002SR2004YA2006FA2008TA2010HI2012HG2014EI2016YR2018RR2020GW2022LT2024SE2026GK2028EI2030KN2032KD2034EI2036LA2038LL2040KA2042LF2044LP2046KR2048LS2050II2052HC2054665-7018Reverse transcriptase6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tccaggacatcaaaagggacacagcgccgaggctagaggcaaccggatggctgaccaagcggcccgaaaggcagccatcacagagactccagacacctctaccctcctcatagaaaattcatcaccctctggcggctcaaaaagaaccgccgacggcagcgaattcgagcccaagaggtcctgtagttttccctgtgtcgcggctccgatctccgttggcctaccgactggttcgccgggctttccgtcggtagtgtctctgaggtctgtggagatgggaggagtatcttttaagtagtgggagaccgccgagtttttcttggcggctgccgtcgcttaagctcgggttcBseRIEcoRIPG2056HQ2058KG2060HS2062AE2064AR2066GN2068RM2070AD2072QA2074AR2076KA2078AI2080TE2082TP2084DT2086ST2088LL2090IE2092NS2094SP2096SG2098GS2100KR2102TA2104DG2106SE2108FE2110PK2112665-7018Reverse transcriptase6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aagaagaggaaagtctaaccggtcatcatcaccatcaccattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgagaaaatttcttctcctttcagattggccagtagtagtggtagtggtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcttttaAgeIPmeIKK2114RK2116V*2118665-7018bGH poly(A) signal6xHisBGH-revSV40 NLS7,0207,0407,0607,0807,1007,1207,1407,160
    tgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctcgataccgtcgacctctagctagagcacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagctatggcagctggagatcgatctcgBbsISphIbGH poly(A) signal7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctagggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggatcccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcalac promoterM13/pUC ReverseCAP binding siteM13 ReverseM13 revlac operator7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggcggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtccSapIBspQIL44407,5407,5607,5807,6007,6207,6407,6607,6807,700
    aaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgDrdIPciIAflIIIoripBR322ori-F7,7207,7407,7607,7807,8007,8207,8407,860
    tccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgaApaLIBciVIori7,8807,9007,9207,9407,9607,9808,0008,0208,040
    gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacori8,0608,0808,1008,1208,1408,1608,1808,2008,220
    cgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgori8,2408,2608,2808,3008,3208,3408,3608,3808,400
    gaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttccttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagAmpR8,4208,4408,4608,4808,5008,5208,5408,560
    gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaaAhdIAmpR8,5808,6008,6208,6408,6608,6808,7008,7208,740
    atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcctaggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactaggAclIFspIAmpR8,7608,7808,8008,8208,8408,8608,8808,9008,920
    cccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgaggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcPvuIAmpR8,9408,9608,9809,0009,0209,0409,0609,0809,100
    aatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaattatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttAclIApaLIAmpRAmp-R9,1209,1409,1609,1809,2009,2209,2409,260
    ctgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatagactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtatSspIBciVIAmpRAmpR promoter9,2809,3009,3209,3409,3609,3809,4009,4209,440
    tttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcgatctcccgatcccctagggtcgactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgtaaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagctagagggctaggggatcccagctgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaClaIBspDISgrDIAmpR promoterpRS-marker9,4609,4809,5009,5209,5409,5609,5809,6009,620
    tggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcgctacatgcccggtctataMfeINruI9,6409,6609,6809,7009,7209,740
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9753 bp700075008000850090009500500100015002000Cas9M13/pUC ReverseT7 promoter665-7018CMV enhancer,CMV promoter,AflIII,MluI,NdeI,SnaBI,CspCICMV-F,T7,SV40 NLS,NotI,SacIIBglII,PflMIBmgBIM13 Reverse,M13 rev,lac operator,lac promoterCAP binding site,L4440,BspQI,SapIAflIII,PciIpBR322ori-F,DrdI,BciVIApaLIoriAhdIFspI,AclIAmpR,Amp-R,PvuI,AclIAmpR promoter,pRS-marker,ApaLI,SspI,BciVI,SgrDI,BspDI,ClaI,MfeI,NruI

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    9753