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pIB-hbP2+r2-1+3-evePr-MS2v5-LacZ-Tub3UTR
  • Sequence Map
    Editing disabled on read-only sequence.
    gacctacatcacggccgaatgaagggcgaattctgcagatatccatcacactggcggccgccaccgcggtggagctccagcttttgttccctttagtgagggttaatttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacactggatgtagtgccggcttacttcccgcttaagacgtctataggtagtgtgaccgccggcggtggcgccacctcgaggtcgaaaacaagggaaatcactcccaattaaagctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgtEagIEagINotIPstISacIEco53kIpromot...erasecommo...antsThe l...TG).source20406080100120140160
    acatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgctgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgpromoter fo...lac operonsource180200220240260280300320340
    ttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaagPciIsource360380400420440460480500520
    cataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgchigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationsource540560580600620640660680700
    tggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgacchigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationsource720740760780800820840860880
    taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccahigh-copy-number ColE1/pMB1/pBR322/pUC origin of replicationsource9009209409609801,0001,0201,0401,060
    ggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaahigh-copy-number ColE1...origin of replicationsource1,0801,1001,1201,1401,1601,1801,2001,2201,240
    ttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactaAhdIbla CDSsource1,2601,2801,3001,3201,3401,3601,3801,4001,420
    accgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatttggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaaAclIbla CDSsource1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccbla CDSsource1,6201,6401,6601,6801,7001,7201,7401,7601,780
    cagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattgggtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccScaIbla CDSsource1,8001,8201,8401,8601,8801,9001,9201,940
    aaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaattttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttAclIXmnIbla CDSsource1,9601,9802,0002,0202,0402,0602,0802,1002,120
    tgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgCspCIbla promoterf1 bacteriopha...and synthesisbla CDSsource2,1402,1602,1802,2002,2202,2402,2602,2802,300
    gttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggccaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgAloIPsiIf1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesissource2,3202,3402,3602,3802,4002,4202,4402,4602,480
    gatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaactaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttNaeIBsaAIDraIIINgoMIVf1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesissource2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccaf1 bacteriophage origin of rep...ction of (+) strand synthesissource2,6802,7002,7202,7402,7602,7802,8002,8202,840
    aacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattgggtaccgggccccccctcgacgatgtaggtcacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgggctccccgggcgcgtactccacctcttgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacccatggcccgggggggagctgctacatccagtgccagagcttcggcgccacgcccacggtcccgcacgggaacccgaggggcccgcgcatgaggtggagApaIKpnISmaIXmaITspMIAcc65IPspOMIEcoO109Ipromot...erasecommo...antssource2,8602,8802,9002,9202,9402,9602,9803,0003,020
    acccatctggtccatcatgatgaacgggtcgaggtggcggtagttgatcccggcgaacgcgcggcgcaccgggaagccctcgccctcgaaaccgctgggcgcggtggtcacggtgagcacgggacgtgcgacggcgtcggcgggtgcggatacgcggggcagcgtcagcgggttctcgtgggtagaccaggtagtactacttgcccagctccaccgccatcaactagggccgcttgcgcgccgcgtggcccttcgggagcgggagctttggcgacccgcgccaccagtgccactcgtgccctgcacgctgccgcagccgcccacgcctatgcgccccgtcgcagtcgcccaagagcPflFITth111Isource3,0403,0603,0803,1003,1203,1403,1603,1803,200
    acggtcacggcgggcatgtcgaggtcgacggtatcgataagcttgatatcgaattcgaagatctCACGCTAGCTGCCTACTCCTGCTGTCGACTCCTGACCAACGTAATCCCCATAGAAAACCGGTGGAAAATTCGCAGCTCGCTGCTAAGCTGGCCATCCGCTAAGCTCCCGGATCAtgccagtgccgcccgtacagctccagctgccatagctattcgaactatagcttaagcttctagaGTGCGATCGACGGATGAGGACGACAGCTGAGGACTGGTTGCATTAGGGGTATCTTTTGGCCACCTTTTAAGCGTCGAGCGACGATTCGACCGGTAGGCGATTCGAGGGCCTAGTAgeIBmtIClaIMscINheIPfoIBglIIBspDIHindIIITao's hb P2 enhancercommo...antssourcesource3,2203,2403,2603,2803,3003,3203,3403,3603,380
    TCCCAAATCCAAGTGCGCATAATTTTTTGTTTCTGCTCTAATCCAGAATGGATCAAGACCACAATCCTCAATCCGCGATCCGTGATCCTCGATTCCCGACCGATCCGCGACCTGTACCTGACTTGTGGTCACCTCTGCCCATCTAATCCCTTGACGCGTGCATCCGTCTACCTGAGCGAGGGTTTAGGTTCACGCGTATTAAAAAACAAAGACGAGATTAGGTCTTACCTAGTTCTGGTGTTAGGAGTTAGGCGCTAGGCACTAGGAGCTAAGGGCTGGCTAGGCGCTGGACATGGACTGAACACCAGTGGAGACGGGTAGATTAGGGAACTGCGCACGTAGGCAGATGGACTCGCAhdIFspAIBpu10IBstEIITao's hb P2 enhancersource3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ATggcgcgccaactgcagagcgcagcggtataaaagggcgcggggtggctgagagcagcacactcgagctgtgaccgccgcacagtcaacaactaactgccttcgttaatatcctctgaataagccaactttgaatcacaagacgcataccaaacatgcacggataccgaacctacaaTAccgcgcggttgacgtctcgcgtcgccatattttcccgcgccccaccgactctcgtcgtgtgagctcgacactggcggcgtgtcagttgttgattgacggaagcaattataggagacttattcggttgaaacttagtgttctgcgtatggtttgtacgtgcctatggcttggatgttAscIPstITliIXhoIPspXIBssHIIPaeR7Ieve promotersource3,5803,6003,6203,6403,6603,6803,7003,720
    catggagagccaccatgcccatcacgacgccagtcccgtggacctagaATGCATaacctacaaacgggtggaggatcaccccacccgacacttcacaatcaaggggtacaatacacaagggtggaggaacaccccaccctccagacacattacacagaaatccaatcaaacagaagcagtacctctcggtggtacgggtagtgctgcggtcagggcacctggatctTACGTAttggatgtttgcccacctcctagtggggtgggctgtgaagtgttagttccccatgttatgtgttcccacctccttgtggggtgggaggtctgtgtaatgtgtctttaggttagtttgtcttcgtBsmINsiIPshAI24xMS2V5 misc_featureeve promotersource3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ccatcagggcttctgctaccaaatttatctcaaaaaactacaacaaggaatcaccatcagggattccctgtgcaatatacgtcaaacgagggccacgacgggaggacgatcacgcctcccgaatatcggcatgtctggctttcgaattcagtgcgtggagcatcagcccacgcagccaggtagtcccgaagacgatggtttaaatagagttttttgatgttgttccttagtggtagtccctaagggacacgttatatgcagtttgctcccggtgctgccctcctgctagtgcggagggcttatagccgtacagaccgaaagcttaagtcacgcacctcgtagtcgggtgcgtcggt24xMS2V5 misc_featuresource3,9203,9403,9603,9804,0004,0204,0404,0604,080
    atcagagtcgaatacaagtcgactttcgcgaagagcatcagccttcgcgccattcttacacaaaccacactctcccctacaggaacagcatcagcgttcctgcccagtacccaactcaagaaaatttatgtccccatgcagcatcagcgcatgggccccaagaatacatccccaacaatagtctcagcttatgttcagctgaaagcgcttctcgtagtcggaagcgcggtaagaatgtgtttggtgtgagaggggatgtccttgtcgtagtcgcaaggacgggtcatgggttgagttcttttaaatacaggggtacgtcgtagtcgcgtacccggggttcttatgtaggggttgttApaINruIPspOMIEcoO109I24xMS2V5 misc_featuresource4,1004,1204,1404,1604,1804,2004,2204,2404,260
    aatcacatccgagcaccaacagggctcggagtgttgtttcttgtccaactggacaaaccctccatggaccatcaggccatggactctcaccaacaagacaaaaactactcttctcgaagcagcatcagcgcttcgaaacactcgagcatacattgtgcctatttcttgggtggacgatttagtgtaggctcgtggttgtcccgagcctcacaacaaagaacaggttgacctgtttgggaggtacctggtagtccggtacctgagagtggttgttctgtttttgatgagaagagcttcgtcgtagtcgcgaagctttgtgagctcgtatgtaacacggataaagaacccacctgctaNcoINcoITliIXhoIPspXIPaeR7I24xMS2V5 misc_featuresource4,2804,3004,3204,3404,3604,3804,4004,4204,440
    cacgccacccatgctctcacgaatttcaaaacacggacaaggacgagcaccaccagggctcgtcgttccacgtccaatacgattacttacctttcgggatcacgatcacggatcccgcagctacatcacttccactcaggacattcaagcatgcacgatcacggcatgctccacaagtgtgcggtgggtacgagagtgcttaaagttttgtgcctgttcctgctcgtggtggtcccgagcagcaaggtgcaggttatgctaatgaatggaaagccctagtgctagtgcctagggcgtcgatgtagtgaaggtgagtcctgtaagttcgtacgtgctagtgccgtacgaggtgttcaBamHI24xMS2V5 misc_featuresource4,4604,4804,5004,5204,5404,5604,5804,6004,620
    ctcaaccacagaaactaccaaatgggttcagcaccagcgaacccactcctacctcaaacctcttcccacaaaactggcaagcaggatcaccgcttgcccattccaacataccaaatcaaaaacaattactggtacagcatcagcgtaccagcccacatctctcactactatcaaaaacgagttggtgtctttgatggtttacccaagtcgtggtcgcttgggtgaggatggagtttggagaagggtgttttgaccgttcgtcctagtggcgaacgggtaaggttgtatggtttagtttttgttaatgaccatgtcgtagtcgcatggtcgggtgtagagagtgatgatagtttttg24xMS2V5 misc_featuresource4,6404,6604,6804,7004,7204,7404,7604,7804,800
    caaaccgttcagcaacagcgaacggtacacacggaaaaatcaactggtttacaaatacgaaagacgatcacgctttcgtccagcgcaaactattacgaaaaacatccgacgggaagagcaacagccttcccgcggcggaaaacctcacaaaaacacgacaaacggatgcacgaacacggtttggcaagtcgttgtcgcttgccatgtgtgcctttttagttgaccaaatgtttatgctttctgctagtgcgaaagcaggtcgcgtttgataatgctttttgtaggctgcccttctcgttgtcggaagggcgccgccttttggagtgtttttgtgctgtttgcctacgtgcttgtgcAloI24xMS2V5 misc_featuresource4,8204,8404,8604,8804,9004,9204,9404,9604,980
    gcatccgccgacaacccacaaacttacaaccaggcaaacggtgcaggatcaccgcaccgtacatcaaacacctcagatctcatACTAGTgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaacgtaggcggctgttgggtgtttgaatgttggtccgtttgccacgtcctagtggcgtggcatgtagtttgtggagtctagagtaTGATCAcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattSpeIBglIIBstAPIlacZ CDS24xMS2V5 misc_featuresource5,0005,0205,0405,0605,0805,1005,1205,1405,160
    tagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcgaaacggaccaaaggccgtggtcttcgccacggcctttcgaccgacctcacgctagaaggactccggctatgacagcagcaggggagtttgaccgtctacgtgccaatgctacgcBaeIBsu36IlacZ CDSsource5,1805,2005,2205,2405,2605,2805,3005,3205,340
    cccatctacaccaacgtaacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgggtagatgtggttgcattggatagggtaatgccagttaggcggcaaacaagggtgcctcttaggctgcccaacaatgagcgagtgtaaattacaactactttcgaccgatgtccttccggtctgcgcttaataaaaactaccgcaattgagccgcaaagtagacaccacgttgcccglacZ CDSsource5,3605,3805,4005,4205,4405,4605,4805,500
    gctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgttggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcacgacccagccaatgccggtcctgtcagcaaacggcagacttaaactggactcgcgtaaaaatgcgcggcctcttttggcggagcgccactaccacgacgcaacctcactgccgtcaatagaccttctagtcctatacaccgcctactcgccgtaaaaggcactgcagagcaacgacgtZraIAatIIBsmBIEsp3IBpu10IlacZ CDSsource5,5205,5405,5605,5805,6005,6205,6405,6605,680
    taaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcatttggctgatgtgtttagtcgctaaaggtacaacggtgagcgaaattactactaaagtcggcgcgacatgacctccgacttcaagtctacacgccgctcaacgcactgatggatgcccattgtcaaagaaataccgtcccactttgcgtccagcggtcgccgtggcgcggaaagccglacZ CDSsource5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggccactttaatagctactcgcaccaccaatacggctagcgcagtgtgatgcagacttgcagcttttgggctttgacacctcgcggctttagggcttagagatagcacgccaccaacttgacgtgtggcggctgccgtgcgactaacttcgtcttcggacgctacagccaaaggcgctccClaIBspDIlacZ CDSsource5,8805,9005,9205,9405,9605,9806,0006,0206,040
    tgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaacgcctaacttttaccagacgacgacgacttgccgttcggcaacgactaagctccgcaattggcagtgctcgtagtaggagacgtaccagtccagtacctactcgtctgctaccacgtcctataggacgactacttcgtcttgttgaaattgcggcacgcgacaagcgtaataggcttlacZ CDSsource6,0606,0806,1006,1206,1406,1606,1806,2006,220
    ccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgggtaggcgacaccatgtgcgacacgctggcgatgccggacatacaccacctacttcggttataactttgggtgccgtaccacggttacttagcagactggctactaggcgcgaccgatggccgctactcgcttgcgcattgcgcttaccacgtcgcgctagcattagtgggctcacacBclIPflMIDraIIIlacZ CDSsource6,2406,2606,2806,3006,3206,3406,3606,3806,400
    atcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcctagtagaccagcgaccccttacttagtccggtgccgcgattagtgctgcgcgacatagcgacctagtttagacagctaggaagggcgggccacgtcatacttccgccgcctcggctgtggtgccggtggctataataaacgggctacatgcgcgcgcacctacttctggtcgggaaggBbsIMauBIBssHIIlacZ CDSsource6,4206,4406,4606,4806,5006,5206,5406,5606,580
    cggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggagccgacacggctttaccaggtagttttttaccgaaagcgatggacctctctgcgcgggcgactaggaaacgcttatgcgggtgcgctacccattgtcagaaccgccaaagcgatttatgaccgtccgcaaagcagtcataggggcaaatgtcccgccgaagcagaccctgacccacctBsmBIEsp3IPflMIlacZ CDSsource6,6006,6206,6406,6606,6806,7006,7206,7406,760
    tcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttaagtcagcgactaatttatactacttttgccgttgggcaccagccgaatgccgccactaaaaccgctatgcggcttgctagcggtcaagacatacttgccagaccagaaacggctggcgtgcggcgtaggtcgcgactgccttcgttttgtggtcgtcgtcaaaaaggtcaaggcaaatlacZ CDSsource6,7806,8006,8206,8406,8606,8806,9006,9206,940
    tccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgaggcccgtttggtagcttcactggtcgcttatggacaaggcagtatcgctattgctcgaggacgtgacctaccaccgcgacctaccattcggcgaccgttcgccacttcacggagacctacagcgaggtgttccatttgtcaactaacttgacggacttgatggcgtcggcctctcgcSacIEco53kIlacZ CDSsource6,9606,9807,0007,0207,0407,0607,0807,1007,120
    ccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttgcatcgaggcccgttgagaccgagtgtcatgcgcatcacgttggcttgcgctggcgtaccagtcttcggcccgtgtagtcgcggaccgtcgtcaccgcagaccgccttttggagtcacactgcgaggggcggcgcagggtgcggtagggcgtagactggtggtcgctttacctaaaaacgtagctlacZ CDSsource7,1407,1607,1807,2007,2207,2407,2607,280
    gctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgccgacccattattcgcaaccgttaaattggcggtcagtccgaaagaaagtgtctacacctaaccgctattttttgttgacgactgcggcgacgcgctagtcaagtgggcacgtggcgacctattgctgtaaccgcattcacttcgctgggcgtaactgggattgcggacccagcttgcglacZ CDSsource7,3007,3207,3407,3607,3807,4007,4207,4407,460
    tggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgaccttccgccgcccggtaatggtccggcttcgtcgcaacaacgtcacgtgccgtctatgtgaacgactacgccacgactaatgctggcgagtgcgcaccgtcgtagtccccttttggaataaatagtcggccttttggatggcctaactaccatcaccagtttaccgctaatggcaaclacZ CDSsource7,4807,5007,5207,5407,5607,5807,6007,6207,640
    atgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtatacccctacaacttcaccgctcgctatgtggcgtaggccgcgcctaaccggacttgacggtcgaccgcgtccatcgtctcgcccatttgaccgagcctaatcccggcgttcttttgatagggctggcggaatgacggcggacaaaactggcgaccctagacggtaacagtctgtacatatggggBbsIPciIBstZ17IlacZ CDSsource7,6607,6807,7007,7207,7407,7607,7807,8007,820
    gtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaatatcgaccatgcagaagggctcgcttttgccagacgcgacgccctgcgcgcttaacttaataccgggtgtggtcaccgcgccgctgaaggtcaagttgtagtcggcgatgtcagttgtcgttgactacctttggtcggtagcggtagacgacgtgcgccttcttccgtgtaccgacttatagctgBsiWIlacZ CDSsource7,8407,8607,8807,9007,9207,9407,9607,9808,000
    ggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaattccagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataataataaccgggcaggccatgtctgcccgtatttcgcgtaaggaaatccattatgtactatttaaaaaccaaaggtatacccctaaccaccgctgctgaggacctcgggcagtcatagccgccttaaggtcgactcgcggccagcgatggtaatggtcaaccagaccacagtttttattattattggcccgtccggtacagacgggcataaagcgcattcctttaggtaatacatgataaatttttNdeIPfoIlacZ CDSsource8,0208,0408,0608,0808,1008,1208,1408,1608,180
    acacaaacttttggatgttcggtttattctttttcttttacttttttatcatgggagcctacttcccgtttttcccgatttggctacatgacatcaaccatatcagcaaaagtgatacgggtattatttttgccgctatttctctgttctcgctattattccaaccgctgtttggtcttgtgtttgaaaacctacaagccaaataagaaaaagaaaatgaaaaaatagtaccctcggatgaagggcaaaaagggctaaaccgatgtactgtagttggtatagtcgttttcactatgcccataataaaaacggcgataaagagacaagagcgataataaggttggcgacaaaccagasource8,2008,2208,2408,2608,2808,3008,3208,3408,360
    gctttctgacaaactcggcaagctctaggcccactaagcgtcgcgccacttcaacgctcgatgggagcgtcattggtgggcggggtaaccgtcgaaatcagtgtttacgcttccaatcgcaacaaaaaattcactgcaacactgaaaagcatacgaaaacgatgaagattgtacgagacgaaagactgtttgagccgttcgagatccgggtgattcgcagcgcggtgaagttgcgagctaccctcgcagtaaccacccgccccattggcagctttagtcacaaatgcgaaggttagcgttgttttttaagtgacgttgtgacttttcgtatgcttttgctacttctaacatgctctBstEIIsource8,3808,4008,4208,4408,4608,4808,5008,5208,540
    aaccataaagtattttatccacaaagacacgtatagcagaaaagccaagttaactcggcgataagttgtgtacacaagaataaaatcggccagattcagtgttgtcagaaataagaaaaccccactatgtttttctttgccttttctttctcccagcgatcattcatttcgtggtgaattggtatttcataaaataggtgtttctgtgcatatcgtcttttcggttcaattgagccgctattcaacacatgtgttcttattttagccggtctaagtcacaacagtctttattcttttggggtgatacaaaaagaaacggaaaagaaagagggtcgctagtaagtaaagcaccacttBsaAIBsrGIsource8,5608,5808,6008,6208,6408,6608,6808,7008,720
    agaacggggtcattgcacggagtttcgactgcgggaaagcagagctgccgttcacttcgtctataattagcgctttctattttccccgattcgggccgctgctgcgcttttccgcctgctgtttgtggcaagtgtagcagcaggctgtgcacgcaggtggcatgcccgccgtgaccgttcttgccccagtaacgtgcctcaaagctgacgccctttcgtctcgacggcaagtgaagcagatattaatcgcgaaagataaaaggggctaagcccggcgacgacgcgaaaaggcggacgacaaacaccgttcacatcgtcgtccgacacgtgcgtccaccgtacgggcggcactggcaPaqCIBstAPIsource8,7408,7608,7808,8008,8208,8408,8608,8808,900
    cgagaacccgctgacgctgccccgcgtatccgcacccgccgacgccgtcgcacgtcccgtgctcaccgtgaccaccgcgcccagcggtttcgagggcgagggcttcccggtgcgccgcgcgttcgccgggatcaactaccgccacctcgacccgttcatcatgatggaccagatgggtgctcttgggcgactgcgacggggcgcataggcgtgggcggctgcggcagcgtgcagggcacgagtggcactggtggcgcgggtcgccaaagctcccgctcccgaagggccacgcggcgcgcaagcggccctagttgatggcggtggagctgggcaagtagtactacctggtctacccaPflFITth111Isource8,9208,9408,9608,9809,0009,0209,0409,060
    gaggtggagtacgcgcccggggagcccaagggcacgccctggcacccgcaccgcggcttcgagaccctccacctcatgcgcgggcccctcgggttcccgtgcgggaccgtgggcgtggcgccgaagctctggSmaIXmaITspMIsource9,0809,1009,1209,140
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    9144 bp65007000750080008500500100015002000sourcelacZ CDS1.11-P2PSeq7 (DNA)2.13-MCPGFPSeq5 (DNA)promoter for bacteriophage T3 RNA polymerase,+13PciIhigh-copy-number ColE1/pMB1/pBR322/pUC origin of replication3.30-Carnegie4Seq5 (DNA)AhdIAclIbla CDSScaIAclIXmnIbla promoter14.2-eveSeqUp2 (DNA)1.102-P2PSeq15 (DNA)Eco53kISacIPciI,BstZ17I,BbsI,BsiWI1.6-P2PSeq3 (DNA)NdeI,PfoI1.8-P2PSeq4 (DNA),1.10-P2PSeq6 (DNA)1.9-P2PSeq5 (DNA),1.26-P2PSeq10 (DNA),2.36-Tub3-SeqUpstream (DNA),BstEII,+23.32-TubUpSeq1 (DNA),PaqCI,BstAPI,PflFI,Tth111I,TspMI,XmaI,SmaI

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    9144