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pFAB5491 (Dubseq vector v1, no fragments) (Public)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    ccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtSacIScaITatIBanIIBtgZIEcoRVBsiHKAIEco53kICmRT020406080100120140160
    gcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtDraIMscINcoIPasISspIBsmBIEsp3IPflMICmR180200220240260280300320340
    cgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagAclIApoIBpmIBsaWIBspEIBtgZIBtsCICmR360380400420440460480500520
    atcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccDraIFokIPvuIICmR540560580600620640660680700
    tatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccagatatcgacgtcgacaccatcgaatggtgcatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacggctagttgcagagtaaaagcggtctatagctgcagctgtggtagcttaccacgAccIMslISalIZraIAatIIBsaHIBsmBIEcoRVEsp3IPflMIBpu10IHincIICmR720740760780800820840860
    aaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcataatacgactcactatagggtggtgaatgatgtccacgaggtctctnnnnnnnnnnnnnnnnnnnncgtacgctgcaggtcgaccacgtgtaagtaagtaagaaaacgagctcgaattcatcgnnnttttggaaagcgccataccgtactatcgcgggccttctctcagttaagtattatgctgagtgatatcccaccacttactacaggtgctccagagannnnnnnnnnnnnnnnnnnngcatgcgacgtccagctggtgcacattcattcattcttttgctcgagcttaagtagcnnnAccIApoIBsaIEarIPmlIPstISacISalIBanIIBsiWIBssSIEcoRIAflIIIBssSαIHincIIBsiHKAIEco53kITake this!FW primi...ownTag)T7PromoterFW Bar...te-U1j5_000...rwardRV Bar...te-U2j5_...se3 ...p8809009209409609801,0001,0201,040
    nnnnnnnnnnnnnnnnnctacgagaccgacaccgaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacctagnnnnnnnnnnnnnnnnngatgctctggctgtggctcctagaggtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgagtggaagcccacccggaaagacgcaaatatggatcBmrIBpmIBsaIPsrIAvrIIdbl termTake this!j5_00001_(pBbB...bone)_forwardj5_00004_(p...2)_reverseRV Pr...tag)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gctacagccgatagtctggaacagcgcacttacgggttgctgcgcaacccaagtgctaccggcgcggcagcgtgacccgtgtcggcggctccaacggctcgccatcgtccagaaaacacggctcatcgggcatcggcaggcgctgctgcccgcgccgttcccattcctccgtttccgatgtcggctatcagaccttgtcgcgtgaatgcccaacgacgcgttgggttcacgatggccgcgccgtcgcactgggcacagccgccgaggttgccgagcggtagcaggtcttttgtgccgagtagcccgtagccgtccgcgacgacgggcgcggcaagggtaaggaggcaaagFspIAlwNIPshAIBstAPIEcoP15IBBR1 ori1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggtcaaggctggcaggtctggttccatgcccggaatgccgggctggctgggcggctcctcgccggggccggtcggtagttgctgctcgcccggatacagggtcgggatgcggcgcaggtcgccatgccccaacagcgattcgtcctggtcgtcgtgatcaaccaccacggcggcaccagttccgaccgtccagaccaaggtacgggccttacggcccgaccgacccgccgaggagcggccccggccagccatcaacgacgagcgggcctatgtcccagccctacgccgcgtccagcggtacggggttgtcgctaagcaggaccagcagcactagttggtggtgccgccgtBclIBglIFokIBciVIBseRIBseYIBsiEIBtsCIBBR1 ori1,4201,4401,4601,4801,5001,5201,5401,560
    ctgaacaccgacaggcgcaactggtcgcggggctggccccacgccacgcggtcattgaccacgtaggccaacacggtgccggggccgttgagcttcacgacggagatccagcgctcggccaccaagtccttgactgcgtattggaccgtccgcaaagaacgtccgatgagcttgggacttgtggctgtccgcgttgaccagcgccccgaccggggtgcggtgcgccagtaactggtgcatccggttgtgccacggccccggcaactcgaagtgctgcctctaggtcgcgagccggtggttcaggaactgacgcataacctggcaggcgtttcttgcaggctactcgaaccAfeIBbsIAvaIINmeAIIIBBR1 ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aaagtgtcttctggctgaccaccacggcgttctggtggcccatctgcgccacgaggtgatgcagcagcattgccgccgtgggtttcctcgcaataagcccggcccacgcctcatgcgctttgcgttccgtttgcacccagtgaccgggcttgttcttggcttgaatgccgatttctttcacagaagaccgactggtggtgccgcaagaccaccgggtagacgcggtgctccactacgtcgtcgtaacggcggcacccaaaggagcgttattcgggccgggtgcggagtacgcgaaacgcaaggcaaacgtgggtcactggcccgaacaagaaccgaacttacggctaaagBmrIBssSIBssSαIEcoP15IBBR1 ori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tctggactgcgtggccatgcttatctccatgcggtaggggtgccgcacggttgcggcaccatgcgcaatcagctgcaacttttcggcagcgcgacaacaattatgcgttgcgtaaaagtggcagtcaattacagattttctttaacctacgcaatgagctattgcggggggtgccagacctgacgcaccggtacgaatagaggtacgccatccccacggcgtgccaacgccgtggtacgcgttagtcgacgttgaaaagccgtcgcgctgttgttaatacgcaacgcattttcaccgtcagttaatgtctaaaagaaattggatgcgttactcgataacgccccccacggFspIMscIPvuIIBBR1 ori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcaatgagctgttgcgtaccccccttttttaagttgttgatttttaagtctttcgcatttcgccctatatctagttctttggtgcccaaagaagggcacccctgcggggttcccccacgccttcggcgcggctccccctccggcaaaaagtggcccctccggggcttgttgatcgcgttactcgacaacgcatggggggaaaaaattcaacaactaaaaattcagaaagcgtaaagcgggatatagatcaagaaaccacgggtttcttcccgtggggacgccccaagggggtgcggaagccgcgccgagggggaggccgtttttcaccggggaggccccgaacaactagcBaeGIBaeGIBBR1 ori2,1202,1402,1602,1802,2002,2202,2402,260
    actgcgcggccttcggccttgcccaaggtggcgctgcccccttggaacccccgcactcgccgccgtgaggctcggggggcaggcgggcgggcttcgcccttcgactgcccccactcgcataggcttgggtcgttccaggcgcgtcaaggccaagccgctgcgcggtcgctgcgcgtgacgcgccggaagccggaacgggttccaccgcgacgggggaaccttgggggcgtgagcggcggcactccgagccccccgtccgcccgcccgaagcgggaagctgacgggggtgagcgtatccgaacccagcaaggtccgcgcagttccggttcggcgacgcgccagcgacgcgcAvaIHgaIBsiEIBsoBIBBR1 ori2,2802,3002,3202,3402,3602,3802,4002,4202,440
    agccttgacccgccttccacttggtgtccaaccggcaagcgaagcgcgcaggccgcaggccggaggcactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaattcggaactgggcggaaggtgaaccacaggttggccgttcgcttcgcgcgtccggcgtccggcctccgtgatcacgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaSpeIBssHIIBBR1 oriT02,4602,4802,5002,5202,5402,560
    • Plasmid
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    • Linear Map
    Editing is disabled because sequence edits have been locked.
    2579 bp175020002250250500BBR1 oriCmRT0Eco53kI,BanII,BsiHKAI,SacI,EcoRV,BtgZI,TatI,ScaISspI,NcoIMscIDraI,PasI,PflMI,BsmBI,Esp3IBpmIAclIBtgZIApoI,BsaWI,BspEI,BtsCIFokIDraIPvuIIMscIFspIPvuIIBaeGIBaeGIAvaI,BsoBIHgaIBsiEI,BssHII,SpeI

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    2579