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MK Lab 10his-pfu-sso7d-pET28
  • Sequence Map
    Editing disabled on read-only sequence.
    tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctaccgcttaccctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaf1 ori20406080100120140160
    ttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagaAloIBsaXIDraIIIf1 ori180200220240260280300320340
    cggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatgccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttaf1 ori360380400420440460480500520
    acattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatgtaagtttatacataggcgagtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatPflMIkanR540560580600620640660680700
    tcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcaagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtNsiIkanR720740760780800820840860
    tcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaataagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatBsmIPvuIAsiSIkanR8809009209409609801,0001,0201,040
    cctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactcggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagAvaIBsmINsiISmaIXmaIBsoBITspMIkanR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacClaINruIBspDIkanR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgattcgtctgtcaaaataacaagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcori1,4201,4401,4601,4801,5001,5201,5401,560
    gatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgatactagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctatAcuIAlwNIori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    agtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggctcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgcBssSIBssSαIori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgacggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactPciIAflIII2,1202,1402,1602,1802,2002,2202,2402,260
    tgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgPflFITth111Ibom2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctccccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagPfoIrop2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccaatgactactacttrop2,6402,6602,6802,7002,7202,7402,7602,7802,800
    catgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcggtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccagttacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctacgtctaggccttgtattaccacgtcccgcBspEI2,8202,8402,8602,8802,9002,9202,9402,960
    ctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgtcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggggcggtcggatcggcccaggagttgctgtcPpuMI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgctcgtgctagtacgcgtgggcaccccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccctggtcactgcttccgaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcBglIFspIFspAI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacatttttactgggtctcgcgacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaPshAI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    taattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactcBsaXIlacI3,5203,5403,5603,5803,6003,6203,6403,660
    agagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctataggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcgHpaIBsrDIEcoRVBssHIIHincIIlacI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    catctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtlacI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccApaIBstEIIPspOMINmeAIIIlacI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    catcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgBclIMluIBsrDIAflIIIlacI4,2204,2404,2604,2804,3004,3204,3404,360
    gtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataacacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattlacI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggPfoIPflMIBstAPIlacI promoterlacI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggtcatcatccaactccggcaactcgtggcggcggcgttccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccSphI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatggggcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagagctagggcgctttaattatgctgagtgatatccccttaacactcgcctattgttaaggggagatctttattaaaacaaattgaaattcttcctctatatggtacccBarINcoIStyIXbaIBglIISgrAIMG2lac operatorRBST7 promoter4,9204,9404,9604,9805,0005,0205,0405,060
    ccatcatcatcatcatcatcatcatcatcacagcagcggccatatcgaaggtcgtcatatgattttagatgtggattacataactgaagaaggaaaacctgttattaggctattcaaaaaagagaacggaaaatttaagatagagcatgatagaacttttagaccatacatttacggtagtagtagtagtagtagtagtagtagtgtcgtcgccggtatagcttccagcagtatactaaaatctacacctaatgtattgacttcttccttttggacaataatccgataagttttttctcttgccttttaaattctatctcgtactatcttgaaaatctggtatgtaaatgAcuINdeIHH4HH6HH8HH10HH12SS14GH16IE18GR20HM22IL24DV26DY28IT30EE32GK34PV36IR38LF40KK42EN44GK46FK48IE50HD52RT54FR56PY58IY605071-7668Pfu10xHisFle...erFac...te5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gctcttctcagggatgattcaaagattgaagaagttaagaaaataacgggggaaaggcatggaaagattgtgagaattgttgatgtagagaaggttgagaaaaagtttctcggcaagcctattaccgtgtggaaactttatttggaacatccccaagatgttcccactattagagcgagaagagtccctactaagtttctaacttcttcaattcttttattgccccctttccgtacctttctaacactcttaacaactacatctcttccaactctttttcaaagagccgttcggataatggcacacctttgaaataaaccttgtaggggttctacaagggtgataatctcNmeAIIIAL62LR64DD66SK68IE70EV72KK74IT76GE78RH80GK82IV84RI86VD88VE90KV92EK94KF96LG98KP100IT102VW104KL106YL108EH110PQ112DV114PT116IR1185071-7668Pfu5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aaaaagttagagaacatccagcagttgtggacatcttcgaatacgatattccatttgcaaagagatacctcatcgacaaaggcctaataccaatggagggggaagaagagctaaagattcttgccttcgatatagaaaccctctatcacgaaggagaagagtttggaaaaggccctttttcaatctcttgtaggtcgtcaacacctgtagaagcttatgctataaggtaaacgtttctctatggagtagctgtttccggattatggttacctcccccttcttctcgatttctaagaacggaagctatatctttgggagatagtgcttcctcttctcaaaccttttccgggStuIBstBIEK120VR122EH124PA126VV128DI130FE132YD134IP136FA138KR140YL142ID144KG146LI148PM150EG152EE154EL156KI158LA160FD162IE164TL166YH168EG170EE172FG174KG176P5071-7668Pfu5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aattataatgattagttatgcagatgaaaatgaagcaaaggtgattacttggaaaaacatagatcttccatacgttgaggttgtatcaagcgagagagagatgataaagagatttctcaggattatcagggagaaggatcctgacattatagttacttataatggagactcattcttaatattactaatcaatacgtctacttttacttcgtttccactaatgaacctttttgtatctagaaggtatgcaactccaacatagttcgctctctctctactatttctctaaagagtcctaatagtccctcttcctaggactgtaatatcaatgaatattacctctgagtaagBamHIBglIII178IM180IS182YA184DE186NE188AK190VI192TW194KN196ID198LP200YV202EV204VS206SE208RE210MI212KR214FL216RI218IR220EK222DP224DI226IV228TY230NG232DS234F5071-7668Pfu5,6205,6405,6605,6805,7005,7205,7405,760
    gacttcccatatttagcgaaaagggcagaaaaacttgggattaaattaaccattggaagagatggaagcgagcccaagatgcagagaataggcgatatgacggctgtagaagtcaagggaagaatacatttcgacttgtatcatgtaataacaaggacaataaatctcccaacatctgaagggtataaatcgcttttcccgtctttttgaaccctaatttaattggtaaccttctctaccttcgctcgggttctacgtctcttatccgctatactgccgacatcttcagttcccttcttatgtaaagctgaacatagtacattattgttcctgttatttagagggttgtaD236FP238YL240AK242RA244EK246LG248IK250LT252IG254RD256GS258EP260KM262QR264IG266DM268TA270VE272VK274GR276IH278FD280LY282HV284IT286RT288IN290LP292T5071-7668Pfu5,7805,8005,8205,8405,8605,8805,9005,9205,940
    acacactagaggctgtatatgaagcaatttttggaaagccaaaggagaaggtatacgccgacgagatagcaaaagcctgggaaagtggagagaaccttgagagagttgccaaatactcgatggaagatgcaaaggcaacttatgaactcgggaaagaattccttccaatggaaattgtgtgatctccgacatatacttcgttaaaaacctttcggtttcctcttccatatgcggctgctctatcgttttcggaccctttcacctctcttggaactctctcaacggtttatgagctaccttctacgtttccgttgaatacttgagccctttcttaaggaaggttacctttaAvaIBsoBIEcoRIY294TL296EA298VY300EA302IF304GK306PK308EK310VY312AD314EI316AK318AW320ES322GE324NL326ER328VA330KY332SM334ED336AK338AT340YE342LG344KE346FL348PM350EI3525071-7668Pfu5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tcagctttcaagattagttggacaacctttatgggatgtttcaaggtcaagcacagggaaccttgtagagtggttcttacttaggaaagcctacgaaagaaacgaagtagctccaaacaagccaagtgaagaggagtatcaaagaaggctcagggagagctacacaggtggattcagtcgaaagttctaatcaacctgttggaaataccctacaaagttccagttcgtgtcccttggaacatctcaccaagaatgaatcctttcggatgctttctttgcttcatcgaggtttgttcggttcacttctcctcatagtttcttccgagtccctctcgatgtgtccacctaagQL354SR356LV358GQ360PL362WD364VS366RS368ST370GN372LV374EW376FL378LR380KA382YE384RN386EV388AP390NK392PS394EE396EY398QR400RL402RE404SY406TG408GF4105071-7668Pfu6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gttaaagagccagaaaaggggttgtgggaaaacatagtatacctagattttagagccctatatccctcgattataattacccacaatgtttctcccgatactctaaatcttgagggatgcaagaactatgatatcgctcctcaagtaggccacaagttctgcaaggacatccctgcaatttctcggtcttttccccaacacccttttgtatcatatggatctaaaatctcgggatatagggagctaatattaatgggtgttacaaagagggctatgagatttagaactccctacgttcttgatactatagcgaggagttcatccggtgttcaagacgttcctgtagggacEcoRVVK412EP414EK416GL418WE420NI422VY424LD426FR428AL430YP432SI434II436TH438NV440SP442DT444LN446LE448GC450KN452YD454IA456PQ458VG460HK462FC464KD466IP4685071-7668Pfu6,3206,3406,3606,3806,4006,4206,4406,460
    gttttataccaagtctcttgggacatttgttagaggaaagacaaaagattaagacaaaaatgaaggaaactcaagatcctatagaaaaaatactccttgactatagacaaaaagcgataaaactcttagcaaattctttctacggatattatggctatgcaaaagcaagatggtacaaaatatggttcagagaaccctgtaaacaatctcctttctgttttctaattctgtttttacttcctttgagttctaggatatcttttttatgaggaactgatatctgtttttcgctattttgagaatcgtttaagaaagatgcctataataccgatacgttttcgttctaccatGF470IP472SL474LG476HL478LE480ER482QK484IK486TK488MK490ET492QD494PI496EK498IL500LD502YR504QK506AI508KL510LA512NS514FY516GY518YG520YA522KA524RW526Y5071-7668Pfu6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ctgtaaggagtgtgctgagagcgttactgcctggggaagaaagtacatcgagttagtatggaaggagctcgaagaaaagtttggatttaaagtcctctacattgacactgatggtctctatgcaactatcccaggaggagaaagtgaggaaataaagaaaaaggctctagaatttgacattcctcacacgactctcgcaatgacggaccccttctttcatgtagctcaatcataccttcctcgagcttcttttcaaacctaaatttcaggagatgtaactgtgactaccagagatacgttgatagggtcctcctctttcactcctttatttctttttccgagatcttaaaBsaIDraISacIXbaIEco53kIC528KE530CA532ES534VT536AW538GR540KY542IE544LV546WK548EL550EE552KF554GF556KV558LY560ID562TD564GL566YA568TI570PG572GE574SE576EI578KK580KA582LE584F5071-7668Pfu6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gtaaaatacataaattcaaagctccctggactgctagagcttgaatatgaagggttctataagaggggattcttcgttacgaagaagaggtatgcagtaatagatgaagaaggaaaagtcattactcgtggtttagagatagttaggagagattggagtgaaattgcaaaagaaacattttatgtatttaagtttcgagggacctgacgatctcgaacttatacttcccaagatattctcccctaagaagcaatgcttcttctccatacgtcattatctacttcttccttttcagtaatgagcaccaaatctctatcaatcctctctaacctcactttaacgttttctttBssSIBssSαIV586KY588IN590SK592LP594GL596LE598LE600YE602GF604YK606RG608FF610VT612KK614RY616AV618ID620EE622GK624VI626TR628GL630EI632VR634RD636WS638EI640AK642E5071-7668Pfu6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctcaagctagagttttggagacaatactaaaacacggagatgttgaagaagctgtgagaatagtaaaagaagtaatacaaaagcttgccaattatgaaattccaccagagaagctcgcaatatatgagcagataacaagaccattacatgagtataaggcgataggtcctcacgtgagttcgatctcaaaacctctgttatgattttgtgcctctacaacttcttcgacactcttatcattttcttcattatgttttcgaacggttaatactttaaggtggtctcttcgagcgttatatactcgtctattgttctggtaatgtactcatattccgctatccaggagtgcaPpuMIHindIIIT644QA646RV648LE650TI652LK654HG656DV658EE660AV662RI664VK666EV668IQ670KL672AN674YE676IP678PE680KL682AI684YE686QI688TR690PL692HE694YK696AI698GP700HV7025071-7668Pfu7,0207,0407,0607,0807,1007,1207,1407,160
    agctgttgcaaagaaactagctgctaaaggagttaaaataaagccaggaatggtaattggatacatagtacttagaggcgatggtccaattagcaatagggcaattctagctgaggaatacgatcccaaaaagcacaagtatgacgcagaatattacattgagaaccaggttctttcgacaacgtttctttgatcgacgatttcctcaattttatttcggtccttaccattaacctatgtatcatgaatctccgctaccaggttaatcgttatcccgttaagatcgactccttatgctagggtttttcgtgttcatactgcgtcttataatgtaactcttggtccaagaaScaIBbvCISexAIAV704AK706KL708AA710KG712VK714IK716PG718MV720IG722YI724VL726RG728DG730PI732SN734RA736IL738AE740EY742DP744KK746HK748YD750AE752YY754IE756NQ758VL7605071-7668Pfu7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ccagcggtacttaggatattggagggatttggatacagaaaggaagacctcagataccaaaagacaagacaagtcggcctaacttcctggcttaacattaaaaaatccggtaccggcggtggcggtgcaaccgtaaagttcaagtacaaaggcgaagaaaaagaggtagacatctggtcgccatgaatcctataacctccctaaacctatgtctttccttctggagtctatggttttctgttctgttcagccggattgaaggaccgaattgtaattttttaggccatggccgccaccgccacgttggcatttcaagttcatgtttccgcttctttttctccatctgtagaKpnIAcc65IPA762VL764RI766LE768GF770GY772RK774ED776LR778YQ780KT782RQ784VG786LT788SW790LN792IK794KS796GT798GG800GG802AT804VK806FK808YK810GE812EK814EV816DI8185071-7668PfuSso7ddu Overlap7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ccaagatcaagaaagtatggcgtgtgggcaagatgatctccttcacctacgacgagggcggtggcaagaccggccgtggtgcggtaagcgaaaaggacgcgccgaaggagctgctgcagatgctggagaagcagaaaaagtgataactgctaacaaagcccgaaaggaagctgagggttctagttctttcataccgcacacccgttctactagaggaagtggatgctgctcccgccaccgttctggccggcaccacgccattcgcttttcctgcgcggcttcctcgacgacgtctacgacctcttcgtctttttcactattgacgattgtttcgggctttccttcgactcEagIPstIAlwNISK820IK822KV824WR826VG828KM830IS832FT834YD836EG838GG840KT842GR844GA846VS848EK850DA852PK854EL856LQ858ML860EK862QK864K*8665071-7668Sso7d7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggataaccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctaBlpIStyIBspEIT7 terminator7,7207,7407,7607,7807,800
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7800 bp5500600065007000750050010001500Pfuori5071-7668f1 ori,DraIII,BsaXI,AloIPflMINsiIkanR,AsiSI,PvuI,BsmI,BsmI,AvaI,BsoBI,TspMI,XmaI,SmaI,NsiIBspDI,ClaINruIAcuIAlwNIAvaIBsoBIEcoRIEcoRVEco53kI,SacI,DraI,BsaI,XbaIBssSI,BssSαIHindIII,PpuMI,ScaI,BbvCI,SexAIdu Overlap,Sso7d,T7 terminator,Acc65I,KpnI,EagI,PstI,AlwNI,BlpI,StyI,BspEI

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    7800