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LSM052 CMV-TO-CCR6-PCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHIfrom LSM040pUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBmrIBsaIAmp (bla)from LSM040180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspINmeAIIIAmp (bla)from LSM040360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)from LSM040540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIXmnIAmp (bla)from LSM040720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoterfrom LSM0408809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactBspHISgrDIbla promoterfrom LSM0401,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIAlwNIBsiWIAflIIIBpu10Ifrom LSM0401,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)from LSM0401,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaBmrINdeICMVCMV promoter (with TetOs)from LSM0401,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggNcoICspCISnaBICMVCMV promoter (with TetOs)from LSM0401,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetOfrom LSM0401,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatATGAGCGGAGAGAGCATGAACTTCAGTGATGTGTTCGATTCCgccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctaTACTCGCCTCTCTCGTACTTGAAGTCACTACACAAGCTAAGGClaIPmeIAflIIBspDIBpu10IHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28SG30ES32MN34FS36DV38FD40S2153-4219from xz032from XZ032Signal/FlagCCR6CMV pro...TetOs)homologfrom LSM0402,1202,1402,1602,1802,2002,2202,2402,260
    AGTGAGGACTACTTCGTGTCCGTCAATACATCCTACTACAGCGTAGATTCTGAGATGCTGCTTTGCTCCCTGCAGGAGGTGCGCCAGTTCTCCCGACTCTTCGTTCCCATCGCGTATTCACTCATCTGCGTCTTTGGCCTCCTGGGGAATATCTTGGTAGTCATCACATTTGCTTTCACTCCTGATGAAGCACAGGCAGTTATGTAGGATGATGTCGCATCTAAGACTCTACGACGAAACGAGGGACGTCCTCCACGCGGTCAAGAGGGCTGAGAAGCAAGGGTAGCGCATAAGTGAGTAGACGCAGAAACCGGAGGACCCCTTATAGAACCATCAGTAGTGTAAACGAAPstISbfIS42ED44YF46VS48VN50TS52YY54SV56DS58EM60LL62CS64LQ66EV68RQ70FS72RL74FV76PI78AY80SL82IC84VF86GL88LG90NI92LV94VI96TF98A2153-4219from xz032from XZ032CCR62,2802,3002,3202,3402,3602,3802,4002,4202,440
    TTTATAAAAAGGCCAGATCCATGACAGACGTCTACCTGCTTAATATGGCCATTGCAGACATCCTGTTCGTCCTGACGCTCCCATTCTGGGCTGTGAGTCACGCTACCGGGGCCTGGGTGTTCAGTAATGCAACCTGCAAGCTGTTGAAGGGAATTTATGCCATCAACTTTAATTGAAATATTTTTCCGGTCTAGGTACTGTCTGCAGATGGACGAATTATACCGGTAACGTCTGTAGGACAAGCAGGACTGCGAGGGTAAGACCCGACACTCAGTGCGATGGCCCCGGACCCACAAGTCATTACGTTGGACGTTCGACAACTTCCCTTAAATACGGTAGTTGAAATTAACPsiIF100YK102KA104RS106MT108DV110YL112LN114MA116IA118DI120LF122VL124TL126PF128WA130VS132HA134TG136AW138VF140SN142AT144CK146LL148KG150IY152AI154NF156NC1582153-4219from xz032from XZ032CCR62,4602,4802,5002,5202,5402,5602,5802,6002,620
    CGGTATGCTGCTGCTGACTTGCATAAGTATGGACAGATACATCGCCATAGTACAGGCAACAAAATCCTTCAGACTGAGGTCTCGAACGCTCCCTAGGTCTAAAATCATTTGTCTTGTTGTATGGGGCTTGAGCGTGATTATTTCCAGCAGTACTTTCGTGTTTAACCAGAAGTACGCCATACGACGACGACTGAACGTATTCATACCTGTCTATGTAGCGGTATCATGTCCGTTGTTTTAGGAAGTCTGACTCCAGAGCTTGCGAGGGATCCAGATTTTAGTAAACAGAACAACATACCCCGAACTCGCACTAATAAAGGTCGTCATGAAAGCACAAATTGGTCTTCATGBcgIBsaIAvrIIGM160LL162LT164CI166SM168DR170YI172AI174VQ176AT178KS180FR182LR184SR186TL188PR190SK192II194CL196VV198WG200LS202VI204IS206SS208TF210VF212NQ214KY2162153-4219from xz032from XZ032CCR62,6402,6602,6802,7002,7202,7402,7602,7802,800
    AACACGCAGGGCAGCGATGTGTGTGAGCCCAAGTACCAGACGGTATCTGAGCCTATCAGGTGGAAGCTGTTGATGCTGGGTTTGGAATTGCTGTTCGGCTTTTTTATCCCACTCATGTTTATGATTTTTTGTTATACCTTCATCGTGAAGACCCTGGTCCAGGCACAAAACTCTATTGTGCGTCCCGTCGCTACACACACTCGGGTTCATGGTCTGCCATAGACTCGGATAGTCCACCTTCGACAACTACGACCCAAACCTTAACGACAAGCCGAAAAAATAGGGTGAGTACAAATACTAAAAAACAATATGGAAGTAGCACTTCTGGGACCAGGTCCGTGTTTTGAGATAloINT218QG220SD222VC224EP226KY228QT230VS232EP234IR236WK238LL240ML242GL244EL246LF248GF250FI252PL254MF256MI258FC260YT262FI264VK266TL268VQ270AQ272NS2742153-4219from xz032from XZ032CCR62,8202,8402,8602,8802,9002,9202,9402,960
    AGCGACACAAGGCCATCCGGGTAATCATCGCTGTTGTGCTGGTATTTCTGGCTTGCCAGATCCCTCATAATATGGTCCTTCTGGTAACGGCAGCCAACCTGGGCAAAATGAATCGCTCATGTCAGTCAGAAAAGCTTATCGGGTACACCAAAACGGTTACGGAGGTTCTGGCGTTTCGCTGTGTTCCGGTAGGCCCATTAGTAGCGACAACACGACCATAAAGACCGAACGGTCTAGGGAGTATTATACCAGGAAGACCATTGCCGTCGGTTGGACCCGTTTTACTTAGCGAGTACAGTCAGTCTTTTCGAATAGCCCATGTGGTTTTGCCAATGCCTCCAAGACCGCAAHindIIIKR276HK278AI280RV282II284AV286VL288VF290LA292CQ294IP296HN298MV300LL302VT304AA306NL308GK310MN312RS314CQ316SE318KL320IG322YT324KT326VT328EV330LA332F2153-4219from xz032from XZ032CCR62,9803,0003,0203,0403,0603,0803,1003,1203,140
    CCTGCATTGTTGCCTTAACCCTGTGCTGTACGCATTTATAGGTCAGAAGTTTCGAAACTACTTCCTGAAGATTCTCAAAGACCTCTGGTGTGTAAGAAGAAAGTATAAAAGTAGTGGATTTTCATGCGCAGGACGCTACTCCGAAAACATTTCCAGACAGACCAGTGAGACGGCCGGACGTAACAACGGAATTGGGACACGACATGCGTAAATATCCAGTCTTCAAAGCTTTGATGAAGGACTTCTAAGAGTTTCTGGAGACCACACATTCTTCTTTCATATTTTCATCACCTAAAAGTACGCGTCCTGCGATGAGGCTTTTGTAAAGGTCTGTCTGGTCACTCTGCCGGFspIBstBIL334HC336CL338NP340VL342YA344FI346GQ348KF350RN352YF354LK356IL358KD360LW362CV364RR366KY368KS370SG372FS374CA376GR378YS380EN382IS384RQ386TS388ET390A2153-4219from xz032from XZ032CCR63,1603,1803,2003,2203,2403,2603,2803,3003,320
    GATAACGATAACGCCTCCTCCTTTACCATGaccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgACCgGATCCggcCTAGCTTCTAAAACAATAGTGTTGTCTGTAGGAGAAGCCACCAGAACTATTGCTATTGCGGAGGAGGAAATGGTACtggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcTGGcCTAGGccgGATCGAAGATTTTGTTATCACAACAGACATCCTCTTCGGTGGTCTTAgeIBsgIKflIXcmIBamHID392ND394NA396SS398FT400MT402GG404RT406PP408SL410GP412QD414ES416CT418TA420SS422SL424AK426DT428SS430TG432SG434LA436SK438TI440VL442SV444GE446AT448R2153-4219from xz032from XZ032V2 tailPCPCCR6homologsho...erfrom LSM0403,3403,3603,3803,4003,4203,4403,4603,4803,500
    CCCTCACGGAGATCCAAAGCACAGCGGATAGACAAATTTTCGAAGAGAAAGTGGGACCGCTTGTCGGAAGACTCAGGTTGACAGCTTCCCTGCGGCAGAATGGCGCTAAGACCGCTTATAGGGTGAATCTCAAACTTGATCAAGCAGATGTTGTTGATAGCGGCTTGCCCAAAGTGGGAGTGCCTCTAGGTTTCGTGTCGCCTATCTGTTTAAAAGCTTCTCTTTCACCCTGGCGAACAGCCTTCTGAGTCCAACTGTCGAAGGGACGCCGTCTTACCGCGATTCTGGCGAATATCCCACTTAGAGTTTGAACTAGTTCGTCTACAACAACTATCGCCGAACGGGTTTCAAhdIBclIBstBIT450LT452EI454QS456TA458DR460QI462FE464EK466VG468PL470VG472RL474RL476TA478SL480RQ482NG484AK486TA488YR490VN492LK494LD496QA498DV500VD502SG504LP506KV5082153-4219PCPfrom LSM0403,5203,5403,5603,5803,6003,6203,6403,660
    ACGATATACTCAAGTTTGGTCACATGATGTGACAATCGTTGCAAACTCAACGGAAGCGAGTAGGAAAAGCCTTTATGATCTCACTAAGTCACTCGTCGCCACCTCTCAAGTTGAGGACCTTGTCGTCAATTTGGTGCCTCTTGGTCGGGGAGGCGGTTCCGGCGGGGGTACCCTTTGCTATATGAGTTCAAACCAGTGTACTACACTGTTAGCAACGTTTGAGTTGCCTTCGCTCATCCTTTTCGGAAATACTAGAGTGATTCAGTGAGCAGCGGTGGAGAGTTCAACTCCTGGAACAGCAGTTAAACCACGGAGAACCAGCCCCTCCGCCAAGGCCGCCCCCATGGGAAKpnIAcc65IRY510TQ512VW514SH516DV518TI520VA522NS524TE526AS528RK530SL532YD534LT536KS538LV540AT542SQ544VE546DL548VV550NL552VP554LG556RG558GG560SG562GG564TL5662153-4219PCPfrom LSM0403,6803,7003,7203,7403,7603,7803,8003,8203,840
    GCCAGTAAGACCATCGTGTTGAGCGTGGGAGAGGCAACCCGCACACTGACAGAAATCCAGTCAACAGCAGACAGACAGATTTTTGAGGAGAAAGTGGGTCCGCTTGTCGGGCGGCTGCGGCTGACGGCTTCATTGCGACAGAACGGAGCCAAAACTGCCTATCGAGTCAACCTTACGGTCATTCTGGTAGCACAACTCGCACCCTCTCCGTTGGGCGTGTGACTGTCTTTAGGTCAGTTGTCGTCTGTCTGTCTAAAAACTCCTCTTTCACCCAGGCGAACAGCCCGCCGACGCCGACTGCCGAAGTAACGCTGTCTTGCCTCGGTTTTGACGGATAGCTCAGTTGGAATAS568KT570IV572LS574VG576EA578TR580TL582TE584IQ586ST588AD590RQ592IF594EE596KV598GP600LV602GR604LR606LT608AS610LR612QN614GA616KT618AY620RV622NL6242153-4219PCPfrom LSM0403,8603,8803,9003,9203,9403,9603,9804,0004,020
    AACTCGATCAAGCGGATGTTGTGGATTCCGGTCTGCCAAAAGTACGGTACACGCAGGTGTGGTCCCATGACGTGACCATTGTGGCGAACAGTACCGAGGCGTCTCGCAAAAGCCTTTACGATTTGACCAAATCACTCGTTGCAACTTCACAGGTGGAAGACCTGGTTGTCAATCTTTGAGCTAGTTCGCCTACAACACCTAAGGCCAGACGGTTTTCATGCCATGTGCGTCCACACCAGGGTACTGCACTGGTAACACCGCTTGTCATGGCTCCGCAGAGCGTTTTCGGAAATGCTAAACTGGTTTAGTGAGCAACGTTGAAGTGTCCACCTTCTGGACCAACAGTTAGAAleIBmgBIPaqCISexAIKL626DQ628AD630VV632DS634GL636PK638VR640YT642QV644WS646HD648VT650IV652AN654ST656EA658SR660KS662LY664DL666TK668SL670VA672TS674QV676ED678LV680VN682L2153-4219PCPfrom LSM0404,0404,0604,0804,1004,1204,1404,1604,1804,200
    TGTACCTTTGGGCAGGtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgACATGGAAACCCGTCCactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacApaIAvaIBclIPmeITliIXhoIBsoBIPaqCIPspXIPaeR7IPspOMIV684PL686GR688*2153-4219BGH polyadenylation sequencec-myc epitopePolyhi...e tagPCPfrom LSM0404,2204,2404,2604,2804,3004,3204,3404,360
    ccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctaggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatNspISphIBGH polyadenylation sequencefrom LSM0404,3804,4004,4204,4404,4604,4804,5004,5204,540
    tggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgcacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgClaIBspDIPvuIIBGH pAfrom LSM0404,5604,5804,6004,6204,6404,6604,6804,7004,720
    tcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataaKflINaeINgoMIVFRTfrom LSM0404,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtgagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccaBarIXbaIHygromycinFRTfrom LSM0404,9204,9404,9604,9805,0005,0205,0405,060
    aaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgtttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacEcoRIDraIIIHygromycinfrom LSM0405,0805,1005,1205,1405,1605,1805,2005,2205,240
    cccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatgggggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactaccNcoINdeIPstIPvuIAsiSIRsrIIHygromycinfrom LSM0405,2605,2805,3005,3205,3405,3605,3805,4005,420
    acgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagDraIIINmeAIIIHygromycinfrom LSM0405,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagAjuISacIIHygromycinfrom LSM0405,6205,6405,6605,6805,7005,7205,7405,760
    gatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttaHygromycinfrom LSM0405,7805,8005,8205,8405,8605,8805,9005,9205,940
    agcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagtcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcNaeIPfoIPsiINgoMIVSV40 pAfrom LSM0405,9605,9806,0006,0206,0406,0606,0806,1006,120
    catcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgBsmIBstZ17ISV40 pAfrom LSM0406,1406,1606,1806,2006,2206,2406,2606,2806,300
    aacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaPvuIIfrom LSM0406,3206,3406,3606,3806,4006,4206,4406,460
    ccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgNspIPciISapIBspQIAflIIIfrom LSM040pUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctccctcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggafrom LSM040pUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccfrom LSM040pUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttgAlwNIfrom LSM040pUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    caccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttfrom LSM040pUC ori7,1807,2007,2207,240
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7242 bp500055006000650050010001500from LSM040HygromycinCMV promoter (with TetOs)CMVSV40 pAbla promoterAmp (bla)pUC oriEKo152.2 (DNA)oLSM0285 (DNA)XZp046.1 (DNA)XZp031.2 (DNA)pNK018 (DNA)EKo326.3 (DNA)oLSM-pUC_ori_F (DNA)pUCoriR (DNA)BspHI,AhdI,BmrI,BsaINmeAIII,FspI,AclIPvuIBcgIAclIXmnISspIBspHISgrDIFlpInF1 (DNA),AlwNIFlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),MfeI,+2EKo279.1 (DNA),EKo272.1 (DNA),AflIII,MluI,SpeIEK_CMVFa (DNA)EK_CMVFb (DNA)EK_CMVFc (DNA),NdeIEK_CMVFd (DNA),BmrISnaBIEK_CMVFe (DNA),NcoI,CspCINmeAIII,DraIIIAjuISacIIHyg_F (DNA)PfoI,NgoMIV,NaeISV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiI,BsmIM13R Genewiz (DNA),BstZ17IM13-48REV Genewiz (DNA)PvuIIpUCori5F (DNA),BspQI,SapIAflIIIoLSM-pUC_ori_R (DNA),PciI,NspIAlwNI

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