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PCR Template (pBAD Vector)
  • Sequence Map
    Editing disabled on read-only sequence.
    AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTTCTTTGGTTAACAGGTATAACGTAGTCTGTAACGGCAGTGACGCAGAAAATGACCGAGAAGAGCGATTGGTTTGGCCATTGGGGCGAATAATTTTCGTAAGACATTGTTTCGCCCTGGTTTCGGTACTGTTTTTGCGCATTGTTTTCACAGATATTAGTGCCGTCTTTTCAGGTGTAAgeIBsmIMluISapIBspQIAflIIIBstEIIaraO2araO120406080100120140160
    TGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGGGGGTTCTCATCATCATCATCATCATGGTATGGCACTAATAAACGTGCCGCAGTGTGAAACGATACGGTATCGTAAAAATAGGTATTCTAATCGCCTAGGATGGACTGCGAAAAATAGCGTTGAGAGATGACAAAGAGGTATGGGCAAAAAACCCGATTGTCCTCCTTAATTGGTACCCCCCAAGAGTAGTAGTAGTAGTAGTACCATACCGBtgINcoINheIStyIBamHIBsaXIAraI1I2ARA_promoterpBAD_fwd_primerCAP_BS180200220240260280300320340
    TAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCGATGGGGATCCGAGCTCGAGATCTGCAGCTGGTACCATATGGGAATTCGAAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGATCGTACTGACCACCTGTCGTTTACCCAGCCCTAGACATGCTGCTACTGCTATTCCTAGCTACCCCTAGGCTCGAGCTCTAGACGTCGACCATGGTATACCCTTAAGCTTCGAACCGACAAAACCGCCTACTCTCTTCTAAAAGTCGGACTATGTCTAATTTAGTCTTGCGTCTTCGCAvaIBanIBmtIClaIKpnINdeIPstISacITliIXhoIXmnIBamHIBanIIBglIIBsoBIBspDIBstBIEcoRIPvuIIAcc65IPaeR7IEco53kIHindIIIT7_leaderXpress_EKpBAD_r...rimerpTrcHi...rimerXpr...er360380400420440460480500520
    GTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCCAGACTATTTTGTCTTAAACGGACCGCCGTCATCGCGCCACCAGGGTGGACTGGGGTACGGCTTGAGTCTTCACTTTGCGGCATCGCGGCTACCATCACACCCCAGAGGGGTACGCTCTCATCCCTTGACGGTCCGTAGTTTATTTTGCTTTCCGAGTCAGCTTTCTGACCCGGAAAGBsaIrrnB_terminatorrrnB_T1_terminator540560580600620640660680700
    GTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACACAAAATAGACAACAAACAGCCACTTGCGAGAGGACTCATCCTGTTTAGGCGGCCCTCGCCTAAACTTGCAACGCTTCGTTGCCGGGCCTCCCACCGCCCGTCCTGCGGGCGGTATTTGACGGTCCGTAGTTTAATTCGTCTTCCGGTAGGACTGCCTACCGGAAAAACGCAAAGATGTrrnB_T2_terminator720740760780800820840860880
    AACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACTTGAGAAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGSspIBspHIAmpicillinAmpR_promoter9009209409609801,0001,0201,0401,060
    GCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAACGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCACAACTGCGGCCCGTTXmnIBssSIBssSαIHincIIAmpicillin1,0801,1001,1201,1401,1601,1801,2001,2201,240
    GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGEaeIPvuIScaIAmpicillin1,2601,2801,3001,3201,3401,3601,3801,4001,420
    TAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAAAseIFspIAmpicillin1,4401,4601,4801,5001,5201,5401,5601,5801,600
    AATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTTTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAAhdIBglIBpmIBsaINmeAIIIAmpicillin1,6201,6401,6601,6801,7001,7201,7401,7601,780
    CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGBanIBspHIAmpicillin1,8001,8201,8401,8601,8801,9001,9201,940
    CTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCpBR322_origin1,9601,9802,0002,0202,0402,0602,0802,1002,120
    CTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCMmeIAlwNIpBR322_origin2,1402,1602,1802,2002,2202,2402,2602,2802,300
    GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTMmeIBseYIBssSIBssSαIpBR322_origin2,3202,3402,3602,3802,4002,4202,4402,4602,480
    CGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAPciIAflIIIpBR322_origin2,5002,5202,5402,5602,5802,6002,6202,6402,660
    CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATACCNdeISapIBspQIBstAPI2,6802,7002,7202,7402,7602,7802,8002,8202,840
    TGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCAccIPfoIBsaAIBsmBIEsp3IPflFIBstZ17ITth111I2,8602,8802,9002,9202,9402,9602,9803,0003,020
    CTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGTCTAGTTAAGCGCGCGCTTCCGCTTCGCCGTACGTATTACACGGACAGTTTACCTGCTTCGTCCCTAAGACGTTTGGGATACGATGAGGCAGTTCGGCAGTTAACAGACTAAGCAATGGTTAATACTGNsiISphIMauBIBssHIIBstAPIpGEX_...imer3,0403,0603,0803,1003,1203,1403,1603,1803,200
    AACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATTGAACTGCCGATGTAGTAAGTGAAAAAGAAGTGTTGGCCGTGCCTTGAGCGAGCCCGACCGGGGCCACGTAAAAAATTTATGGGCGCTCTTTATCTCAACTAGCAGTTTTGGTTGTAACGCTGGCTGCCACCGCTATCCGTAGGCCCACCACGAGTTTTCGTCGAAGCGGACCGACTAvaIBsoBIAraC3,2203,2403,2603,2803,3003,3203,3403,3603,380
    TACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGCAACCAGGAGCGCGGTCGAATTCTGCGATTAGGGATTGACGACCGCCTTTTCTACACTGTCTGCGCTGCCGCTGTTCGTTTGTACGACACGCTGCGACCGCTATAGTTTTAACGACAGACGGTCCACTAGCGACTACATGACTGTTCGGAGCGCATGGGCTAATAGGTAGCCACCBaeIAflIIEcoRVAraC3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATTACCTCGCTGAGCAATTAGCGAAGGTACGCGGCGTCATTGTTAACGAGTTCGTCTAAATAGCGGTCGTCGAGGCTTATCGCGGGAAGGGGAACGGGCCGCAATTACTAAACGGGTTTGTCCAGCGACTTTACGCCGACCACGCGAAGTAGGCCCGCTTTCTTGGGGCATAACCGTTTAAjuIAraC3,5803,6003,6203,6403,6603,6803,7003,720
    ATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTTAACTGCCGGTCAATTCGGTAAGTACGGTCATCCGCGCGCCTGCTTTCATTTGGGTGACCACTATGGTAAGCGCTCGGAGGCCTACTGCTGGCATCACTACTTAGAGAGGACCGCCCTTGTCGTTTTATAGTGGGCCAGCCGTTTGTTTAAGAGCAGGGACTAAAAAGTGGTGGGGGAEaeINruISspIBspEIBssHIIAraC3,7403,7603,7803,8003,8203,8403,8603,8803,900
    GACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCCTGGCGCTTACCACTCTAACTCTTATATTGGAAAGTAAGGGTCGCCAGCCAGCTATTTTTTTAGCTCTATTGGCAACCGGAGTTAGCCGCAATTTGGGCGGTGGTCTACCCGTAATTTGCTCATAGGGCCGTCGTCCCCTAGTAAAACGCGAAGTCGGTATGAAAAGTATGAGGGCGGBseYIPflMIAraC3,9203,9403,9603,9804,0004,0204,0404,0604,080
    ATTCAGAGTAAGTCTC4,100
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4102 bp30003250350037502505007501000AmpicillinAraCrrnB_T1_terminatorAraI1I2Reverse Primer (DNA)

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    4102