Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pFAB5526 (Dubseq vector v2, no fragments)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cgcgctcctgcggcggcctgtagggcaggctcatacccctgccgaaccgcttttgtcagccggtcggccacggcttccggcgtctcaacgcgctttgagattcccagcttttcggccaatccctgcggtgcataggcgcgtggctcgaccgcttgcgggctgatggtgacgtggcgcgcgaggacgccgccggacatcccgtccgagtatggggacggcttggcgaaaacagtcggccagccggtgccgaaggccgcagagttgcgcgaaactctaagggtcgaaaagccggttagggacgccacgtatccgcgcaccgagctggcgaacgcccgactaccactgcaccgBmgBICloning vector pBBR1MCS-2MOBgene20406080100120140160
    ccactggtggccgctccagggcctcgtagaacgcctgaatgcgcgtgtgacgtgccttgctgccctcgatgccccgttgcagccctagatcggccacagcggccgcaaacgtggtctggtcgcgggtcatctgcgctttgttgccgatgaactccttggccgacagcctgccgtcggtgaccaccggcgaggtcccggagcatcttgcggacttacgcgcacactgcacggaacgacgggagctacggggcaacgtcgggatctagccggtgtcgccggcgtttgcaccagaccagcgcccagtagacgcgaaacaacggctacttgaggaaccggctgtcggacggcagEagINotISfiIBmgBIEcoO109ICloning vector pBBR1MCS-2MOBgene180200220240260280300320340
    ctgcgtcagcggcaccacgaacgcggtcatgtgcgggctggtttcgtcacggtggatgctggccgtcacgatgcgatccgccccgtacttgtccgccagccacttgtgcgccttctcgaagaacgccgcctgctgttcttggctggccgacttccaccattccgggctggccgtcgacgcagtcgccgtggtgcttgcgccagtacacgcccgaccaaagcagtgccacctacgaccggcagtgctacgctaggcggggcatgaacaggcggtcggtgaacacgcggaagagcttcttgcggcggacgacaagaaccgaccggctgaaggtggtaaggcccgaccggcagBspHICloning vector pBBR1MCS-2MOBgene360380400420440460480500520
    atgacgtactcgaccgccaacacagcgtccttgcgccgcttctctggcagcaactcgcgcagtcggcccatcgcttcatcggtgctgctggccgcccagtgctcgttctctggcgtcctgctggcgtcagcgttgggcgtctcgcgctcgcggtaggcgtgcttgagactggccgtactgcatgagctggcggttgtgtcgcaggaacgcggcgaagagaccgtcgttgagcgcgtcagccgggtagcgaagtagccacgacgaccggcgggtcacgagcaagagaccgcaggacgaccgcagtcgcaacccgcagagcgcgagcgccatccgcacgaactctgaccggcCloning vector pBBR1MCS-2MOBgene540560580600620640660680700
    ccacgttgcccattttcgccagcttcttgcatcgcatgatcgcgtatgccgccatgcctgcccctcccttttggtgtccaaccggctcgacgggggcagcgcaaggcggtgcctccggcgggccactcaatgcttgagtatactcactagactttgcttcgcaaagtcgtgaccgggtgcaacgggtaaaagcggtcgaagaacgtagcgtactagcgcatacggcggtacggacggggagggaaaaccacaggttggccgagctgcccccgtcgcgttccgccacggaggccgcccggtgagttacgaactcatatgagtgatctgaaacgaagcgtttcagcactggcBstZ17ICloning vector pBBR1MCS-2MOBgene720740760780800820840860
    cctacggcggctgcggcgccctacgggcttgctctccgggcttcgccctgcgcggtcgctgcgctcccttgccagcccgtggatatgtggacgatggccgcgagcggccaccggctggctcgcttcgctcggcccgtggacaaccctgctggacaagctgatggacaggctgcgcggatgccgccgacgccgcgggatgcccgaacgagaggcccgaagcgggacgcgccagcgacgcgagggaacggtcgggcacctatacacctgctaccggcgctcgccggtggccgaccgagcgaagcgagccgggcacctgttgggacgacctgttcgactacctgtccgacgcgCloning vector pBBR1MCS-28809009209409609801,0001,0201,040
    ctgcccacgagcttgaccacagggattgcccaccggctacccagccttcgaccacatacccaccggctccaactgcgcggcctgcggccttgccccatcaatttttttaattttctctggggaaaagcctccggcctgcggcctgcgcgcttcgcttgccggttggacaccaagtgacgggtgctcgaactggtgtccctaacgggtggccgatgggtcggaagctggtgtatgggtggccgaggttgacgcgccggacgccggaacggggtagttaaaaaaattaaaagagaccccttttcggaggccggacgccggacgcgcgaagcgaacggccaacctgtggttcaCspCICloning vector pBBR1MCS-2BBR1ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggaaggcgggtcaaggctcgcgcagcgaccgcgcagcggcttggccttgacgcgcctggaacgacccaagcctatgcgagtgggggcagtcgaaggcgaagcccgcccgcctgccccccgagcctcacggcggcgagtgcgggggttccaagggggcagcgccaccttgggcaagccttccgcccagttccgagcgcgtcgctggcgcgtcgccgaaccggaactgcgcggaccttgctgggttcggatacgctcacccccgtcagcttccgcttcgggcgggcggacggggggctcggagtgccgccgctcacgcccccaaggttcccccgtcgcggtggaacccgttcAvaIBsoBICloning vector pBBR1MCS-2BBR1ori1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gccgaaggccgcgcagtcgatcaacaagccccggaggggccactttttgccggagggggagccgcgccgaaggcgtgggggaaccccgcaggggtgcccttctttgggcaccaaagaactagatatagggcgaaatgcgaaagacttaaaaatcaacaacttaaaaaaggggggtcggcttccggcgcgtcagctagttgttcggggcctccccggtgaaaaacggcctccccctcggcgcggcttccgcacccccttggggcgtccccacgggaagaaacccgtggtttcttgatctatatcccgctttacgctttctgaatttttagttgttgaattttttccccccaCloning vector pBBR1MCS-2BBR1ori1,4201,4401,4601,4801,5001,5201,5401,560
    acgcaacagctcattgcggcaccccccgcaatagctcattgcgtaggttaaagaaaatctgtaattgactgccacttttacgcaacgcataattgttgtcgcgctgccgaaaagttgcagctgattgcgcatggtgccgcaaccgtgcggcaccctaccgcatggagataagcattgcgttgtcgagtaacgccgtggggggcgttatcgagtaacgcatccaatttcttttagacattaactgacggtgaaaatgcgttgcgtattaacaacagcgcgacggcttttcaacgtcgactaacgcgtaccacggcgttggcacgccgtgggatggcgtacctctattcgtaCloning vector pBBR1MCS-2BBR1ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ggccacgcagtccagagaaatcggcattcaagccaagaacaagcccggtcactgggtgcaaacggaacgcaaagcgcatgaggcgtgggccgggcttattgcgaggaaacccacggcggcaatgctgctgcatcacctcgtggcgcagatgggccaccagaacgccgtggtggtcccggtgcgtcaggtctctttagccgtaagttcggttcttgttcgggccagtgacccacgtttgccttgcgtttcgcgtactccgcacccggcccgaataacgctcctttgggtgccgccgttacgacgacgtagtggagcaccgcgtctacccggtggtcttgcggcaccaccagMscICloning vector pBBR1MCS-2BBR1ori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    agccagaagacactttccaagctcatcggacgttctttgcggacggtccaatacgcagtcaaggacttggtggccgagcgctggatctccgtcgtgaagctcaacggccccggcaccgtgtcggcctacgtggtcaatgaccgcgtggcgtggggccagccccgcgaccagttgctcggtcttctgtgaaaggttcgagtagcctgcaagaaacgcctgccaggttatgcgtcagttcctgaaccaccggctcgcgacctagaggcagcacttcgagttgccggggccgtggcacagccggatgcaccagttactggcgcaccgcaccccggtcggggcgctggtcaacgAfeIBbsIAvaIICloning vector pBBR1MCS-2BBR1ori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcctgtcggtgttcagtgccgccgtggtggttgatcacgacgaccaggacgaatcgctgttggggcatggcgacctgcgccgcatcccgaccctgtatccgggcgagcagcaactaccgaccggccccggcgaggagccgcccagccagcccggcattccgggcatggaaccagacggacagccacaagtcacggcggcaccaccaactagtgctgctggtcctgcttagcgacaaccccgtaccgctggacgcggcgtagggctgggacataggcccgctcgtcgttgatggctggccggggccgctcctcggcgggtcggtcgggccgtaaggcccgtaccttggtctBciVIBseRICloning vector pBBR1MCS-2BBR1ori2,1202,1402,1602,1802,2002,2202,2402,260
    cctgccagccttgaccgaaacggaggaatgggaacggcgcgggcagcagcgcctgccgatgcccgatgagccgtgttttctggacgatggcgagccgttggagccgccgacacgggtcacgctgccgcgccggtagcacttgggttgcgcagcaacccgtaagtgcgctgttccaggacggtcggaactggctttgcctccttacccttgccgcgcccgtcgtcgcggacggctacgggctactcggcacaaaagacctgctaccgctcggcaacctcggcggctgtgcccagtgcgacggcgcggccatcgtgaacccaacgcgtcgttgggcattcacgcgacaaggtPshAIBstAPICloning vector pBBR1MCS-2BBR1ori2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gactatcggctgtagccgcctcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattactgatagccgacatcggcggagcggcgggatatggaacagacggaggggcgcaacgcagcgccacgtacctcggcccggtggagctggacttaccttcggccgccgtggagcgattgcctaagtggcaaaaatagtccgagaccctccgtcttatttactagtatagcagttaatCloning vector pBBR1MCS-2BBR1ori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaatttgctttcgaatttctgccattcaatggaggtgcccctctcggactcgtttgaccggagtccgtaaactcttcgtgtgccagtgtgacgaaggccatcagttatttggccatttggtcgttatctgtattcgccgataaattgctgggacgggacttggctgctggcccagcttaaacgaaagcttaaagacggtaagAgeIDrdIBstBIPflFIBpu10IBsu36ITth111ICloning vector pBBR1MCS-22,6402,6602,6802,7002,7202,7402,7602,7802,800
    atccgcttattatcacttattcaggcgtagcaccaggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccctgccactcatcgcagtcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcatcgacgtaggcgaataatagtgaataagtccgcatcgtggtccgcaaattcccgtggttattgacggaatttttttaatgcggggcgggacggtgagtagcgtcagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgtagctgcSspIZraICloning vector pBBR1MCS-2j5_00002_(back...MCS2)_reversej5_0000...orward2,8202,8402,8602,8802,9002,9202,9402,960
    tcgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcataatacgactcactatagggtggtgaatgatgtccacgaggtctctnnnnnnnnnnnnnnnnnnnncgtacgctgcaggtcgaccacgtgtaagtaagtaagaaaaagctgtggtagcttaccacgttttggaaagcgccataccgtactatcgcgggccttctctcagttaagtattatgctgagtgatatcccaccacttactacaggtgctccagagannnnnnnnnnnnnnnnnnnngcatgcgacgtccagctggtgcacattcattcattcttttBcgIPmlIPstISalISalIAatIIBsiWIAflIIIHincIIHincIIj5_0000...orwardFW Bar...te-U1j5_000...rwardRV Bar...te-U2T7Promoterj5_...se3 ...p2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgagctcgaattcatcgnnnnnnnnnnnnnnnnnnnnctacgagaccgacaccgaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcgctcgagcttaagtagcnnnnnnnnnnnnnnnnnnnngatgctctggctgtggctcctagaggtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgagtggaagcccacccgPsrISacIBanIIEcoRIEco53kIdbl termj5_00001_(pBbB...bone)_forwardj5_00004_(p...2)_reverseFW pr...Tag)RV Pr...tag)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctttctgcgtttatacctaggagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgaaagacgcaaatatggatcctcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagAseIAvrIIj5_00001_(backbo...BRMCS2)_forwardj5_00004_(Barcodes)_reversedbl termCloning vector pBBR1MCS-23,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgcatgcataaaaactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataacacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgtacgtatttttgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattAseINsiINspISphIBsaXICloning vector pBBR1MCS-23,5203,5403,5603,5803,6003,6203,6403,660
    tatttgcccatgggggtgggcgaagaactccagcatgagatccccgcgctggaggatcatccagccggcgtcccggaaaacgattccgaagcccaacctttcatagaaggcggcggtggaatcgaaatctcgtgatggcaggttgggcgtcgcttggtcggtcatttcgaaccccataaacgggtacccccacccgcttcttgaggtcgtactctaggggcgcgacctcctagtaggtcggccgcagggccttttgctaaggcttcgggttggaaagtatcttccgccgccaccttagctttagagcactaccgtccaacccgcagcgaaccagccagtaaagcttggggBplIMslINcoIPfoISspIBstBIBstXICloning vector pBBR1MCS-23,6803,7003,7203,7403,7603,7803,8003,8203,840
    agagtcccgctcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccgtctcagggcgagtcttcttgagcagttcttccgctatcttccgctacgcgacgcttagccctcgccgctatggcatttcgtgctccttcgccagtcgggtaagcggcggttcgagaagtcgttatagtgcccatcggttgcgatacaggactatcgccaggcggtgtgggtcggcBplISapIAvaIIBspQIRsrIIKanCloning vector pBBR1MCS-23,8603,8803,9003,9203,9403,9603,9804,0004,020
    gccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacacggtgtcagctacttaggtcttttcgccggtaaaaggtggtactataagccgttcgtccgtagcggtacccagtgctgctctaggagcggcagcccgtacgcgcggaactcggaccgcttgtcaagccgaccgcgctcggggactacgagaagcaggtctagtaggactagctgtMslINcoINspISapISphIBanIIBspQIKanCloning vector pBBR1MCS-24,0404,0604,0804,1004,1204,1404,1604,1804,200
    agaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccatctggccgaaggtaggctcatgcacgagcgagctacgctacaaagcgaaccaccagcttacccgtccatcggcctagttcgcatacgtcggcggcgtaacgtagtcggtactacctatgaaagagccgtcctcgttccactctactgtcctctaggacggggccgtgaagcgggtBciVIKanCloning vector pBBR1MCS-24,2204,2404,2604,2804,3004,3204,3404,360
    atagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctgcagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacactatcgtcggtcagggaagggcgaagtcactgttgcagctcgtgtcgacgcgttccttgcgggcagcaccggtcggtgctatcggcgcgacggagcaggacgtcaagtaagtcccgtggcctgtccagccagaactgtttttcttggcccgcggggacgcgactgtcggccttgtgDrdIMscIPstIPflFITth111IKanCloning vector pBBR1MCS-24,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatcagatcttgatcccctgcgccatcagatccttggcggcaagccgccgtagtctcgtcggctaacagacaacacgggtcagtatcggcttatcggagaggtgggttcgccggcctcttggacgcacgttaggtagaacaagttagtacgctttgctaggagtaggacagagaactagtctagaactaggggacgcggtagtctaggaaccgccgttcEagIXcmIBglIIBsaBIBsaBIKanCloning vector pBBR1MCS-24,5604,5804,6004,6204,6404,6604,6804,7004,720
    aaagccatccagtttactttgcagggcttcccaaccttaccagagggcgccccagctggcaattccggttcgcttgctgtccataaaaccgcccagtctagctatcgccatgtaagcccactgcaagctacctgctttctctttgcgcttgcgttttcccttgtccagatagccctttcggtaggtcaaatgaaacgtcccgaagggttggaatggtctcccgcggggtcgaccgttaaggccaagcgaacgacaggtattttggcgggtcagatcgatagcggtacattcgggtgacgttcgatggacgaaagagaaacgcgaacgcaaaagggaacaggtctatcgggCloning vector pBBR1MCS-24,7404,7604,7804,8004,8204,8404,8604,8804,900
    agtagctgacattcatcccaggtggcacttttcggggaaatgtgcgcgcccgcgttcctgctggcgctgggcctgtttctggcgctggacttcccgctgttccgtcagcagcttttcgcccacggccttgatgatcgcggcggccttggcctgcatatcccgattcaacggcccctcatcgactgtaagtagggtccaccgtgaaaagcccctttacacgcgcgggcgcaaggacgaccgcgacccggacaaagaccgcgacctgaagggcgacaaggcagtcgtcgaaaagcgggtgccggaactactagcgccgccggaaccggacgtatagggctaagttgccggggPasIMOBgeneCloning vector pBBR1MCS-24,9204,9404,9604,9805,0005,0205,0405,060
    agggcgtccagaacgggcttcaggcgctcccgaaggtctcgggccgtctcttgggcttgatcggccttcttgcgcatctcatcccgcaggtcttgcccgaagtccgcgagggcttccagagcccggcagagaacccgaactagccggaagaacgcgtagagtAvaIBsoBIMOBgeneCloning vector pBBR1MCS-25,0805,1005,1205,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    5156 bp37504000425045004750500025050075010001250Cloning vector pBBR1MCS-2BBR1oriKanBmgBIMOBgene,EcoO109I,BmgBI,SfiI,EagI,NotIBspHIBstZ17ICspCIBplIBspQI,SapI,AvaII,RsrIIMslI,NcoI,NspI,SphIBanII,BspQI,SapICloning vector pBBR1MCS-2,BciVIPflFI,Tth111IMscI,PstI,DrdIEagI,BsaBIBglII,BsaBI,XcmIPasI,AvaI,BsoBI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    5156