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EW482 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) [D241C, M243Q] (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggpUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIAmp (bla)180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspIAmp (bla)360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp (bla)720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoter8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactSgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIAlwNIBsiWIBpu10I1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)1,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaCMVCMV promoter (with TetOs)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggNcoICspCISnaBICMVCMV promoter (with TetOs)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacIBsaBIBsaBIBsmBIEsp3ISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccATGTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGgccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggTACAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACPmeIXcmIAflIIBstEIIHindIIIML2HL4DQ6TP8SR10QP12IP14SE16GL18QL20HL22PQ24VL26AD28AV30SR32LV34LG36KF38GD40L2153-4573hADAR2 cat domainfrom original LSM044 (digest same)CMV pro...TetOs)homolog2,1202,1402,1602,1802,2002,2202,2402,260
    ACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTBseRIT42DN44FS46SP48HA50RR52KV54LA56GV58VM60TT62GT64DV66KD68AK70VI72SV74ST76GT78KC80IN82GE84YM86SD88RG90LA92LN94DC96HA98E2153-4573hADAR2 cat domain2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCBclIBsrGII100IS102RR104SL106LR108FL110YT112QL114EL116YL118NN120KD122DQ124KR126SI128FQ130KS132ER134GG136FR138LK140EN142VQ144FH146LY148IS150TS152PC154GD156AR1582153-4573hADAR2 cat domain2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGBcgIPfoIIF160SP162HE164PI166LE168EP170AD172RH174PN176RK178AR180GQ182LR184TK186IE188SG190QG192TI194PV196RS198NA200SI202QA204WD206GV208LQ210GE212RL214LT2162153-4573hADAR2 cat domainC-term ADAR22,6402,6602,6802,7002,7202,7402,7602,7802,800
    ATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGAvaIPstITliIXhoIBamHIBsoBIPpuMIPaeR7IMS218CS220DK222IA224RW226NV228VG230IQ232GS234LL236SI238FV240EP242IY244FS246SI248IL250GS252LY254HG256DH258LS260RA262MY264QR266IS268NI270ED272LP2742153-4573hADAR2 cat domainC-term ADAR22,8202,8402,8602,8802,9002,9202,9402,960
    CTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCAGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTPL276YT278LN280KP282LL284SG286IS288NA290EA292RQ294PG296KA298PN300FS302VN304WT306VG308DS310AI312EV314IN316AT318TG320KD322EL324GR326AS328RL330CK332H2153-4573hADAR2 cat domainC-term ADAR22,9803,0003,0203,0403,0603,0803,1003,1203,140
    CGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACMluIA334LY336CR338WM340RV342HG344KV346PS348HL350LR352SK354IT356KP358NV360YH362ES364KL366AA368KE370YQ372AA374KA376RL378FT380AF382IK384AG386LG388AW390V2153-4573hADAR2 cat domainC-term ADAR23,1603,1803,2003,2203,2403,2603,2803,3003,320
    GAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCGGTACCGGTGGCGGAACCGGTGGCGGGGCTGGCAGGAGTAGGCTTCTGGAAGACTTTAGGAATAATCGCTATCCGAATCTCCAACTCCGTGAAATTGCCGGCCATATCATGGAGTTTTCACAAGACCAACATGGGACTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGCCATGGCCACCGCCTTGGCCACCGCCCCGACCGTCCTCATCCGAAGACCTTCTGAAATCCTTATTAGCGATAGGCTTAGAGGTTGAGGCACTTTAACGGCCGGTATAGTACCTCAAAAGTGTTCTGGTTGTACCCTAgeIAgeIKpnIPflMIAcc65IGR2SR4LL6ED8FR10NN12RY14PN16LQ18LR20EI22AG24HI26ME28FS30QD32QH34G3395-4570E392KP394TE396QD398QF400SL402TP404GT406GG408GT410GG412GA414GR416SR418LL420ED422FR424NN426RY428PN430LQ432LR434EI436AG438HI440ME442FS444QD446QH448G2153-4573G1T2G3G4G5T6G7G8G9A103365-3394PUF-9RhADAR2 cat domainC-term ADAR2short linker3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GCCGGTTCATTCAGCTGAAGTTAGAGAGAGCTACCCCAGCAGAACGACAGCTCGTGTTTAACGAAATCCTGCAAGCGGCATATCAACTGATGGTAGACGTTTTCGGTAACTACGTGATTCAAAAGTTCTTTGAATTTGGATCTCTTGAACAGAAACTCGCTCTTGCCGAACGTATCGGCCAAGTAAGTCGACTTCAATCTCTCTCGATGGGGTCGTCTTGCTGTCGAGCACAAATTGCTTTAGGACGTTCGCCGTATAGTTGACTACCATCTGCAAAAGCCATTGATGCACTAAGTTTTCAAGAAACTTAAACCTAGAGAACTTGTCTTTGAGCGAGAACGGCTTGCATAS36RF38IQ40LK42LE44RA46TP48AE50RQ52LV54FN56EI58LQ60AA62YQ64LM66VD68VF70GN72YV74IQ76KF78FE80FG82SL84EQ86KL88AL90AE92RI943395-4570S450RF452IQ454LK456LE458RA460TP462AE464RQ466LV468FN470EI472LQ474AA476YQ478LM480VD482VF484GN486YV488IQ490KF492FE494FG496SL498EQ500KL502AL504AE506RI5082153-4573PUF-9R3,5203,5403,5603,5803,6003,6203,6403,660
    ACGGGGCCACGTATTATCCCTCGCCTTACAGATGTACGGTTGTCGGGTTATTCAGAAAGCTCTTGAATTCATCCCGTCAGACCAGCAGAATGAAATGGTCCGAGAATTGCATCAACATACGGAACAGCTTGTACAAGACCAATATGGTAACTATGTCATCCAGCACGTATTGGAGTGCCCCGGTGCATAATAGGGAGCGGAATGTCTACATGCCAACAGCCCAATAAGTCTTTCGAGAACTTAAGTAGGGCAGTCTGGTCGTCTTACTTTACCAGGCTCTTAACGTAGTTGTATGCCTTGTCGAACATGTTCTGGTTATACCATTGATACAGTAGGTCGTGCATAACCTCPsrIBsrGIEcoRIRG96HV98LS100LA102LQ104MY106GC108RV110IQ112KA114LE116FI118PS120DQ122QN124EM126VR128EL130HQ132HT134EQ136LV138QD140QY142GN144YV146IQ148HV150LE1523395-4570RG510HV512LS514LA516LQ518MY520GC522RV524IQ526KA528LE530FI532PS534DQ536QN538EM540VR542EL544HQ546HT548EQ550LV552QD554QY556GN558YV560IQ562HV564LE5662153-4573PUF-9R3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CACGGAAGACCGGAAGATAAATCAAAGATTGTTGCCGAGATAAGAGGGAACGTACTTGTCCTGAGTCAGCATAAGTTCGCAAGTAATGTCGTCGAGAAGTGTGTGACCCATGCGAGCAGAACTGAACGGGCAGTGCTTATCGACGAAGTGTGCACGATGAACGATGGTCCTCACTGTGCCTTCTGGCCTTCTATTTAGTTTCTAACAACGGCTCTATTCTCCCTTGCATGAACAGGACTCAGTCGTATTCAAGCGTTCATTACAGCAGCTCTTCACACACTGGGTACGCTCGTCTTGACTTGCCCGTCACGAATAGCTGCTTCACACGTGCTACTTGCTACCAGGAGTGAHG154RP156ED158KS160KI162VA164EI166RG168NV170LV172LS174QH176KF178AS180NV182VE184KC186VT188HA190SR192TE194RA196VL198ID200EV202CT204MN206DG208PH2103395-4570HG568RP570ED572KS574KI576VA578EI580RG582NV584LV586LS588QH590KF592AS594NV596VE598KC600VT602HA604SR606TE608RA610VL612ID614EV616CT618MN620DG622PH6242153-4573PUF-9R3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CAGCGCTGTATACAATGATGAAAGACCAATACGCGAATTATGTGGTGCAGAAGATGATAGATGTGGCTGAACCAGGCCAAAGGAAAATTTGTATGcagGAACTCCATCAACACACCGAGCAGCTGGTTCAAGACCAATACGGGAACTATGTGATTCAACACGTCCTCGAACACGGGTCGCGACATATGTTACTACTTTCTGGTTATGCGCTTAATACACCACGTCTTCTACTATCTACACCGACTTGGTCCGGTTTCCTTTTAAACATACgtcCTTGAGGTAGTTGTGTGGCTCGTCGACCAAGTTCTGGTTATGCCCTTGATACACTAAGTTGTGCAGGAGCTTGTGCCAfeIBsgIBmgBISA212LY214TM216MK218DQ220YA222NY224VV226QK228MI230DV232AE234PG236QR238KI240CM242QE244LH246QH248TE250QL252VQ254DQ256YG258NY260VI262QH264VL266EH268G3395-4570SA626LY628TM630MK632DQ634YA636NY638VV640QK642MI644DV646AE648PG650QR652KI654CM656QE658LH660QH662TE664QL666VQ668DQ670YG672NY674VI676QH678VL680EH682G2153-4573PUF-9R4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ACGACCTGAAGATAAAAGTAAAATCGTCGCGGAAATCCGTGGTAATGTGCTCGTTCTTTCCCAACATAAGTTTGCAAGCAACGTCGTGGAAAAGTGTGTGACACATGCCTCAAGGACAGAAAGGGCGGTCCTCATTGATGAAGTCTGCACAATGAACGATGGCCCCCATAGCGCATGCTGGACTTCTATTTTCATTTTAGCAGCGCCTTTAGGCACCATTACACGAGCAAGAAAGGGTTGTATTCAAACGTTCGTTGCAGCACCTTTTCACACACTGTGTACGGAGTTCCTGTCTTTCCCGCCAGGAGTAACTACTTCAGACGTGTTACTTGCTACCGGGGGTATCGCGTBsgIR270PE272DK274SK276IV278AE280IR282GN284VL286VL288SQ290HK292FA294SN296VV298EK300CV302TH304AS306RT308ER310AV312LI314DE316VC318TM320ND322GP324HS326A3395-4570R684PE686DK688SK690IV692AE694IR696GN698VL700VL702SQ704HK706FA708SN710VV712EK714CV716TH718AS720RT722ER724AV726LI728DE730VC732TM734ND736GP738HS740A2153-4573PUF-9R4,2204,2404,2604,2804,3004,3204,3404,360
    TTGTATACTATGATGAAAGATCAATATGCGAACTACGTTGTCCAGAAAATGATTGACGTGGCTGAGCCAGGACAAAGGAAAATCGTCATGCACAAAATCAGACCACACATCGCAACTCTCAGGAAATACACATATGGTAAACACATACTTGCAAAGCTGGAGAAGTATTACATGAAACATATGATACTACTTTCTAGTTATACGCTTGATGCAACAGGTCTTTTACTAACTGCACCGACTCGGTCCTGTTTCCTTTTAGCAGTACGTGTTTTAGTCTGGTGTGTAGCGTTGAGAGTCCTTTATGTGTATACCATTTGTGTATGAACGTTTCGACCTCTTCATAATGTACTBmgBIL328YT330MM332KD334QY336AN338YV340VQ342KM344ID346VA348EP350GQ352RK354IV356MH358KI360RP362HI364AT366LR368KY370TY372GK374HI376LA378KL380EK382YY384M3395-4570L742YT744MM746KD748QY750AN752YV754VQ756KM758ID760VA762EP764GQ766RK768IV770MH772KI774RP776HI778AT780LR782KY784TY786GK788HI790LA792KL794EK796YY798M2153-4573PUF-9R4,3804,4004,4204,4404,4604,4804,5004,5204,540
    AGAATGGGGTAGACCTGGGCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctccccTCTTACCCCATCTGGACCCGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggaggggApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIK386NG388VD390LG3923395-4570K800NG802VD804LG806*2153-4573BGH polyadenylation sequencec-myc epitopePUF-9RPolyhi...e tag4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggcgcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgSphIBGH polyadenylation sequence4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgClaIBspDIBGH pA4,9204,9404,9604,9805,0005,0205,0405,060
    ccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaagFRT5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacBarIMscIXbaIBsmBIEsp3IPshAIHygromycinFRT5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccggcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcEcoRIBstAPIDraIIIHygromycin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacaNcoIPstIPvuIAsiSIRsrIIHygromycin5,6205,6405,6605,6805,7005,7205,7405,760
    gatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcgggctacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccBstAPIDraIIIHygromycin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcactaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgtAjuISacIIHygromycin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaataaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttScaIHygromycin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgPfoIPsiISV40 pA6,3206,3406,3606,3806,4006,4206,4406,460
    aatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattcttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaagBsmISV40 pA6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgc6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttggagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacPciISapIBspQIpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttcgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagpUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    tcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggpUC ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    actggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaatgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttAlwNIpUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori7,5407,5607,580
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7597 bp550060006500700050010001500HygromycinCMV promoter (with TetOs)CMVAAmp (bla)EKo152.2 (DNA)EWo100.1 (DNA)EWo263.1 (DNA)oLSM0139 (DNA)EKbR (DNA)oLSM0288 (DNA)oLSM-pUC_ori_R (DNA)pUC ori,AhdI,BsaIFspI,AclIPvuIScaI,BcgIAclIXmnIbla promoter,SspISgrDIAlwNIMfeI,Bpu10IBsiWIMluISpeISnaBINcoICspCIBstAPIDraIIIAjuISacIIHyg_F (DNA),ScaIPfoISV40PA_R (DNA),PsiISV40 pABsmIAn-o17 U6_3MS2_Cre_seq (DNA)pUCori5F (DNA),BspQI,SapIoLSM-pUC_ori_F (DNA),PciIpUCoriR (DNA)pUC ori,AlwNI

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    7597