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pCMV_3N39v4_Fc
  • Sequence Map
    Editing disabled on read-only sequence.
    ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctgatctatacaggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacggttcgactagatatgtPvuIM13 fwd20406080100120140160
    ttgaatcaatattggcaattagccatattagtcattggttatatagcataaatcaatattggctattggccattgcatacgttgtatctatatcataatatgtacatttatattggctcatgtccaatatgaccgccatgttgacattgattattgactagttattaatagtaataacttagttataaccgttaatcggtataatcagtaaccaatatatcgtatttagttataaccgataaccggtaacgtatgcaacatagatatagtattatacatgtaaatataaccgagtacaggttatactggcggtacaactgtaactaataactgatcaataattatcattaSpeIBsrGICMV enhancer180200220240260280300320340
    caattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcatCMV enhancer360380400420440460480500520
    tttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtccgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttacgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgaaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcaggcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaatgccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccacSnaBICMV enhancer540560580600620640660680700
    atgcggttttggcagtacaccaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaataaccccgccccgttgacgcaaatgggcggtaggcgtgtactacgccaaaaccgtcatgtggttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattattggggcggggcaactgcgtttacccgccatccgcacatgCspCICMV promoter720740760780800820840860
    ggtgggaggtctatataagcagagctcgtttagtgaaccgtcagaattaagcttgccaccatggaatcacagacccaggtcctcatgtttcttctgctctgggtatctggtgcggccgctCAGTCCACCATTGAGGAACAGGTCAAGACATTTTTGGACAACTTTAACCACAAAGccaccctccagatatattcgtctcgagcaaatcacttggcagtcttaattcgaacggtggtaccttagtgtctgggtccaggagtacaaagaagacgagacccatagaccacgccggcgaGTCAGGTGGTAACTCCTTGTCCAGTTCTGTAAAAACCTGTTGAAATTGGTGTTTCEagINotISacIPshAIEco53kIHindIIIIgk signal3N39v4CMV promoterve...)8809009209409609801,0001,0201,040
    CCGAAGACCTGTTCTATCAAAGTTCACTTGCTTCTTGGAATTATAACACCAATATTACTGAAGAGAATGTCCAAAACATGAATAATGCTGGGGACAAATGGTCTGCCTTTTTAAAGGAACAGTCCACACTTGCCCAAATGTATCCACTACAAGAAATTCAGAATCTCCAAGTCAAGGCTTCTGGACAAGATAGTTTCAAGTGAACGAAGAACCTTAATATTGTGGTTATAATGACTTCTCTTACAGGTTTTGTACTTATTACGACCCCTGTTTACCAGACGGAAAAATTTCCTTGTCAGGTGTGAACGGGTTTACATAGGTGATGTTCTTTAAGTCTTAGAGGTTCAGTTHindIII3N39v41,0601,0801,1001,1201,1401,1601,1801,2001,220
    GCTTCAGCTGCAGGCTCTTCAGCAAAATGGGTCTTCAGTGCTCTCAGAAGACAAGAGCAAACGGTTGAACACAATTCTAAATACAATGAGCACCATCTACTGTACTGGAAAAGTTTGTAACCCAGATAATCCACAAGAATGCTTATTACTTGAACCAGGTTTGAATGAAATAATGCGAAGTCGACGTCCGAGAAGTCGTTTTACCCAGAAGTCACGAGAGTCTTCTGTTCTCGTTTGCCAACTTGTGTTAAGATTTATGTTACTCGTGGTAGATGACATGACCTTTTCAAACATTGGGTCTATTAGGTGTTCTTACGAATAATGAACTTGGTCCAAACTTACTTTATTACAjuI3N39v41,2401,2601,2801,3001,3201,3401,3601,3801,400
    GCAAACAGTTTAGACTACAATGAGAGGCTCTGGGCTTGGGAAAGCTGGAGATCTGAGGTCGGCAAGCAGCTGAGGCCATTATATGAAGAGTATGTGGTCTTGAAAAATGAGATGGCAAGAGCAAATCATTATGAGGACTATGGGGATTATTGGAGAGGAGACTATGAAGTAAATGCGTTTGTCAAATCTGATGTTACTCTCCGAGACCCGAACCCTTTCGACCTCTAGACTCCAGCCGTTCGTCGACTCCGGTAATATACTTCTCATACACCAGAACTTTTTACTCTACCGTTCTCGTTTAGTAATACTCCTGATACCCCTAATAACCTCTCCTCTGATACTTCATTTACBglII3N39v41,4201,4401,4601,4801,5001,5201,5401,560
    GGGTAGATGGCTATGACTACAGCCGCGGCCAGTTGATTGAAGATGTGGAACATACCTTTGAAGAGATTAAACCATTATATGAACATCTTCATGCCTATGTGAGGGCAAAGTTGATGAATGCCTATCCTTCCTATATCAGTCCAATTGGATGCCTCCCTGCTCATTTGCTTGGTGACCCATCTACCGATACTGATGTCGGCGCCGGTCAACTAACTTCTACACCTTGTATGGAAACTTCTCTAATTTGGTAATATACTTGTAGAAGTACGGATACACTCCCGTTTCAACTACTTACGGATAGGAAGGATATAGTCAGGTTAACCTACGGAGGGACGAGTAAACGAACCACTMfeISacII3N39v41,5801,6001,6201,6401,6601,6801,7001,7201,740
    TATGTGGGGTAGATTTTGGACAAATCTGTACTCTTTGACAGTTCCCTTTGGACAGAAACCAAACATAGATGTTACTGATGCAATGGTGGACCAGGCCTGGGATGCACAGAGAATATTCAAGGAGGCCGAGAAGTTCTTTGTATCTGTTGGTCTTCCTAATATGACTCAAGGATTCATACACCCCATCTAAAACCTGTTTAGACATGAGAAACTGTCAAGGGAAACCTGTCTTTGGTTTGTATCTACAATGACTACGTTACCACCTGGTCCGGACCCTACGTGTCTCTTATAAGTTCCTCCGGCTCTTCAAGAAACATAGACAACCAGAAGGATTATACTGAGTTCCTAAGStuI3N39v4PD21,7601,7801,8001,8201,8401,8601,8801,9001,920
    TGGGAAAATTCCATGCTAACGGACCCAGGAAATGTTCAGAAAGCATGCTGCCATCCCACAGCTTGGGACCTGGGGAAGGGCGACTTCAGGATCCTTATGTGCACAAAGGTGACAATGGACGACTTCCTGACAGCTCATCATGAGATGGGGCATATCCAGTATGATATGGCATATGACCCTTTTAAGGTACGATTGCCTGGGTCCTTTACAAGTCTTTCGTACGACGGTAGGGTGTCGAACCCTGGACCCCTTCCCGCTGAAGTCCTAGGAATACACGTGTTTCCACTGTTACCTGCTGAAGGACTGTCGAGTAGTACTCTACCCCGTATAGGTCATACTATACCGTATACBamHI3N39v4PD21,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTGCACAACCTTTTCTGCTAAGAAATGGAGCTAATGAAGGATTCCATGAAGCTGTTGGGGAAATCATGTCACTTTCTGCAGCCACACCTAAGCATTTAAAATCCATTGGTCTTCTGTCACCCGATTTTCAAGAAGACAATGAAACAGAAATAAACTTCCTGCTCAAACAAGCACTGACGTGTTGGAAAAGACGATTCTTTACCTCGATTACTTCCTAAGGTACTTCGACAACCCCTTTAGTACAGTGAAAGACGTCGGTGTGGATTCGTAAATTTTAGGTAACCAGAAGACAGTGGGCTAAAAGTTCTTCTGTTACTTTGTCTTTATTTGAAGGACGAGTTTGTTCGTGAXcmI3N39v4PD22,1202,1402,1602,1802,2002,2202,2402,260
    CACGATTGTTGGGACTCTGCCATTTACTTACATGTTAGAGAAGTGGAGGTGGATGGTCTTTAAAGGGGAAATTCCCAAAGACCAGTGGATGAAAAAGTGGTGGGAGATGAAGCGAGAGATAGTTGGGGTGGTGGAACCTGTGCCCCATGATGAAACATACTGTGACCCCGCATCTGTGCTAACAACCCTGAGACGGTAAATGAATGTACAATCTCTTCACCTCCACCTACCAGAAATTTCCCCTTTAAGGGTTTCTGGTCACCTACTTTTTCACCACCCTCTACTTCGCTCTCTATCAACCCCACCACCTTGGACACGGGGTACTACTTTGTATGACACTGGGGCGTAGAPciIAflIII3N39v42,2802,3002,3202,3402,3602,3802,4002,4202,440
    CTGTTCCATGTTTCTAATGATTACTCATTCATTCGATATTACACAAGGACCCTTTACCAATTCCAGTTTCAAGAAGCACTTTGTCAAGCAGCTAAACATGAAGGCCCTCTGCACAAATGTGACATCTCAAACTCTACAGAAGCTGGACAGAAACTGTTCAATATGCTGAGGCTTGGACAAGGTACAAAGATTACTAATGAGTAAGTAAGCTATAATGTGTTCCTGGGAAATGGTTAAGGTCAAAGTTCTTCGTGAAACAGTTCGTCGATTTGTACTTCCGGGAGACGTGTTTACACTGTAGAGTTTGAGATGTCTTCGACCTGTCTTTGACAAGTTATACGACTCCGAACAjuI3N39v42,4602,4802,5002,5202,5402,5602,5802,6002,620
    GAAAATCAGAACCCTGGACCCTAGCATTGGAAAATGTTGTAGGAGCAAAGAACATGAATGTAAGGCCACTGCTCAACTACTTTGAGCCCTTATTTACCTGGCTGAAAGACCAGAACAAGAATTCTTTTGTGGGATGGAGTACCGACTGGAGTCCATATGCAGACCAAAGCATCAACTTTTAGTCTTGGGACCTGGGATCGTAACCTTTTACAACATCCTCGTTTCTTGTACTTACATTCCGGTGACGAGTTGATGAAACTCGGGAATAAATGGACCGACTTTCTGGTCTTGTTCTTAAGAAAACACCCTACCTCATGGCTGACCTCAGGTATACGTCTGGTTTCGTAGTTEcoRIPshAI3N39v4hA...)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AGTGAGGATAAGCCTAAAATCAGCTCTTGGAGATAAAGCATATGAATGGAACGACAATGAAATGTACCTGTTCCGATCATCTGTTGCATATGCTATGAGGCAGTACTTTTTAAAAGTAAAAAATCAGATGATTCTTTTTGGGGAGGAGGATGTGCGAGTGGCTAATTTGAAACCATCACTCCTATTCGGATTTTAGTCGAGAACCTCTATTTCGTATACTTACCTTGCTGTTACTTTACATGGACAAGGCTAGTAGACAACGTATACGATACTCCGTCATGAAAAATTTTCATTTTTTAGTCTACTAAGAAAAACCCCTCCTCCTACACGCTCACCGATTAAACTTTGGTScaI3N39v4hACE2(1-740)2,8202,8402,8602,8802,9002,9202,9402,960
    AGAATCTCCTTTAATTTCTTTGTCACTGCACCTAAAAATGTGTCTGATATCATTCCTAGAACTGAAGTTGAAAAGGCCATCAGGATGTCCCGGAGCCGTATCAATGATGCTTTCCGTCTGAATGACAACAGCCTAGAGTTTCTGGGGATACAGCCAACACTTGGACCTCCTAACCTCTTAGAGGAAATTAAAGAAACAGTGACGTGGATTTTTACACAGACTATAGTAAGGATCTTGACTTCAACTTTTCCGGTAGTCCTACAGGGCCTCGGCATAGTTACTACGAAAGGCAGACTTACTGTTGTCGGATCTCAAAGACCCCTATGTCGGTTGTGAACCTGGAGGATTGGEcoRV3N39v4hACE2(1-740)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    AGCCCCCTGTTTCCgacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaaTCGGGGGACAAAGGctgttttgagtgtgtacgggtggcacgggtcgtggacttgaggacccccctggcagtcagaaggagaaggggggttttgggttcctgtgggagtactagagggcctggggactccagtgtacgcaccaccacctgcactcggtgcttctgggactccagttBmgBIhIgG1-Fc3N39v4hACE...40)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatccaagttgaccatgcacctgccgcacctccacgtattacggttctgtttcggcgccctcctcgtcatgttgtcgtgcatggcacaccagtcgcaggagtggcaggacgtggtcctgaccgacttaccgttcctcatgttcacgttccagaggttgtttcgggagggtcgggggtagSacIIhIgG1-Fc3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactctcttttggtagaggtttcggtttcccgtcggggctcttggtgtccacatgtgggacgggggtagggccctactcgactggttcttggtccagtcggactggacggaccagtttccgaagatagggtcgctgtagcggcacctcaccctctcgttacccgtcggcctcttgttgaBsrGIhIgG1-Fc3,5203,5403,5603,5803,6003,6203,6403,660
    acaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatgatatgttctggtgcggagggcacgacctgaggctgccgaggaagaaggagatgtcgttcgagtggcacctgttctcgtccaccgtcgtccccttgcagaagagtacgaggcactacgtactccgagacgtgttggtgatgtgcgtcttctcggagagggacagaggcccatttactatPaqCIhIgG1-Fc3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggatcccgggctgactacaaagaccatgacggtgattataaagatcatgacatcgactacaaggatgacgatgacaagtagtgatcccgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaacctagggcccgactgatgtttctggtactgccactaatatttctagtactgtagctgatgttcctactgctactgttcatcactagggcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttBamHID1Y2K3D4H5D6G7D8Y9K10D11H12D13I14D15Y16K17D18D19D20D21K223xFLAGhGH poly(A) signal3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggaCspCIhGH poly(A) signal4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctcccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaBlpIBsmBIBstXIEsp3IhGH poly(A) signal4,2204,2404,2604,2804,3004,3204,3404,360
    gggattacaggcgtgaaccactgctcccttccctgtccttctgattttaaaataactataccagcaggaggacgtccagacacagcataggctacctggccatgcccaaccggtgggacatttgagttgcttgcttggcactgtcctctcatgcgttgggtccactcagtagatgccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaattttattgatatggtcgtcctcctgcaggtctgtgtcgtatccgatggaccggtacgggttggccaccctgtaaactcaacgaacgaaccgtgacaggagagtacgcaacccaggtgagtcatctacAgeIAloIhGH poly(A) signal4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cctgttgaattgggtacgcggccagcttggctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcggacaacttaacccatgcgccggtcgaaccgacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgXcmISV40 promoterhG...l4,5604,5804,6004,6204,6404,6604,6804,7004,720
    atctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaaccSfiISV40 promoter4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aggaattgatcagcttgggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagctccttaactagtcgaaccctagactagttctctgtcctactcctagcaaagcgtactaacttgttctacctaacgtgcgtccaagaggccggcgaacccacctctccgataagccgatactgacccgtgttgtctgttagccgacgagactacggcggcacaaggccgacagtcgBclIBclIEagIBsaBIMI2EQ4DG6LH8AG10SP12AA14WV16ER18LF20GY22DW24AQ26QT28IG30CS32DA34AV36FR38LS40ANeoR/KanR4,9204,9404,9604,9805,0005,0205,0405,060
    gcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcgtccccgcgggccaagaaaaacagttctggctggacaggccacgggacttacttgacgtcctgctccgtcgcgccgatagcaccgaccggtgctgcccgcaaggaacgcgtcgacacgagctgcaacagtgacttcgcccttccctgaccgacgataacccgcttcacggccccKasINarISfoIPflFIPluTITth111IQ42GR44PV46LF48VK50TD52LS54GA56LN58EL60QD62EA64AR66LS68WL70AT72TG74VP76CA78AV80LD82VV84TE86AG88RD90WL92LL94GE96VP98GNeoR/KanR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    caggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatcgtcctagaggacagtagagtggaacgaggacggctctttcataggtagtaccgactacgttacgccgccgacgtatgcgaactaggccgatggacgggtaagctggtggttcgctttgtagcgtagctcgctcgtgcatgagcctaccttcggccagaacagctagtcctactagQ100DL102LS104SH106LA108PA110EK112VS114IM116AD118AM120RR122LH124TL126DP128AT130CP132FD134HQ136AK138HR140IE142RA144RT146RM148EA150GL152VD154QD156DNeoR/KanR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaacctgcttctcgtagtccccgagcgcggtcggcttgacaagcggtccgagttccgcgcgtacgggctgccgctcctagagcagcactgggtaccgctacggacgaacggcttatagtaccaccttttaccggcgaaaagacctaagtagctgacaccggccgacccacaccgcctRsrIIBssHIIL158DE160EH162QG164LA166PA168EL170FA172RL174KA176RM178PD180GE182DL184VV186TH188GD190AC192LP194NI196MV198EN200GR202FS204GF206ID208CG210RL212GV214AD216NeoR/KanR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgggcgatagtcctgtatcgcaaccgatgggcactataacgacttctcgaaccgccgcttacccgactggcgaaggagcacgaaatgccatagcggcgagggctaagcgtcgcgtagcggaagatagcggaagaactgctcaagaagactcgccctgagaccccaagctttactggcBstBIRY218QD220IA222LA224TR226DI228AE230EL232GG234EW236AD238RF240LV242LY244GI246AA248PD250SQ252RI254AF256YR258LL260DE262FF264*NeoR/KanR5,6205,6405,6605,6805,7005,7205,7405,760
    accaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccgggctcgatcccctcgcgagttggttcagtggttcgctgcgggttggacggtagtgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggcccgagctaggggagcgctcaaccaagtcNruI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ctgctgcctgaggctggacgacctcgcggagttctaccggcagtgcaaatccgtcggcatccaggaaaccagcagcggctatccgcgcatccatgcccccgaactgcaggagtggggaggcacgatggccgctttggtcgacccggacgggacgctcctgcgcctgatacagaacgacgacggactccgacctgctggagcgcctcaagatggccgtcacgtttaggcagccgtaggtcctttggtcgtcgccgataggcgcgtaggtacgggggcttgacgtcctcacccctccgtgctaccggcgaaaccagctgggcctgccctgcgaggacgcggactatgtcttgAccISalI5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gaattgcttgcaggcatctcatgagtgtgtcttcccgttttccgcctgaggtcactgcgtggatggagcgctggcgcctgctgcgcgacggcgagctgctcaccacccactcgccaagctggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgccgatcatacttaacgaacgtccgtagagtactcacacagaagggcaaaaggcggactccagtgacgcacctacctcgcgaccgcggacgacgcgctgccgctcgacgagtggtgggtgagcggttcgaccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggctagtatAfeIKasINarISfoIBsaBIPluTIDraIII6,1406,1606,1806,2006,2206,2406,2606,2806,300
    atcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttttttagtcggtatggtgtaaacatctccaaaatgaacgaaattttttggagggtgtggagggggacttggactttgtattttacttacgttaacaacaacaattgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaaHpaIMfeISV40 poly(A) signal6,3206,3406,3606,3806,4006,4206,4406,460
    cactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcaattccctatagtgagtcgtattaaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctagttaagggatatcactcagcataatttaagcattagtacagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttSV40 poly(A) signallac promoterT7 promoterM13 revlac operator6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgaCAP binding site6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctPciIAflIIIori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtori7,0207,0407,0607,0807,1007,1207,1407,160
    cgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagaggcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    cgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttggctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    caagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagori7,5407,5607,5807,6007,6207,6407,6607,6807,700
    taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctc*W286HK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240AmpR7,7207,7407,7607,7807,8007,8207,8407,860
    acccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctactgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgAclIGR238EG236AG234SK232DA230IF228WG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182AmpR7,8807,9007,9207,9407,9607,9808,0008,0208,040
    aggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcatccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtPvuIPM180TT178DR176ED174NP172IA170EN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AmpR8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattgcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaacScaIAS122CL120ER118VT116MG114DT112LH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PAmpR8,2408,2608,2808,3008,3208,3408,3608,3808,400
    gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgcttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcAclIF64RE62EP60RF58SE56LI54KG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RAmpR8,4208,4408,4608,4808,5008,5208,5408,560
    gaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgcgccctgtagcggcgcattaagcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcgcgggacatcgccgcgtaattcgF6HQ4IS2MAmpRAmpR promoterf1 ori8,5808,6008,6208,6408,6608,6808,7008,7208,740
    gcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacccgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggf1 ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    ccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaAloIDraIIIf1 ori8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaaf1 ori9,1209,1409,1609,180
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    9180 bp650070007500800085005001000150020003N39v4oriPD2AmpRM13 fwd,CMV enhancer,PvuI,BsrGI,SpeICMV promoter,SnaBI,CspCI+8HindIII,AjuIBglIISacIIMfeIStuIBamHIXcmIAflIIIPciIAclIPvuI,ScaIAmpR promoter,AclIf1 ori,DraIII,AloI

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    9180