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pTS040(dnaB)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    tatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggactPvuIIBpu10Icat promotorCmR20406080100120140160
    aaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccagatatcgacgtctactagataggcgtatcacgaggcagaTCCTCTGGCGGAAAGCCTctaacatcagtgcctatgctttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacggctagttgcagagtaaaagcggtctatagctgcagatgatctatccgcatagtgctccgtctAGGAGACCGCCTTTCGGAgattgtagtcacggatacgAccIMslIZraIAatIIBsaAIBsaHIBsmBIBssSIEcoRVEsp3IBssSαIdsDNA version for cloning of sinlge mutantscat promotoroligohomolo...30V))sub-li...pacer180200220240260280300320340
    ggacatcgtgcgtgaacgtGTGgttGTAcgtgagatgatctcggttgcgaatgagattgccgaagcgatcTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTtcgcaaccgagatcatctcaGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACcctgtagcacgcacttgcaCACcaaCATgcactctactagagccaacgcttactctaacggcttcgctagAACTGTCGATCGAGTCAGGATCCATATTATGATCAagcgttggctctagtagagtCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGBmtINheISpeIAvrIIBsaAIAflIIIdsDNA version for cloning of sinlge mutantsoligohomology sequence (here: dnaB(A130V))sgRNACas9 handleoverlapPJ23119protospacer360380400420440460480500520
    TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTGAAGCTTGGGCCCGAACAAAAACTCatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagaAACTTTTTCACCGTGGCTCAGCCACGAAAAAAACTTCGAACCCGGGCTTGTTTTTGAGtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgccagaggtcgaaccgacaaaaccgcctactctcttctaaaagtcggactatgtctAccIApaIBanIBpmIBsaIEarIFokIPmeISalIXmnIBaeGIBanIIBtsCIHaeIIHincIIPspOMIHindIIIBsp1286IdsDNA version fo... sinlge mutantssgRNAS. pyogenes terminator540560580600620640660680700
    ttaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggaatttagtcttgcgtcttcgccagactattttgtcttaaacggaccgccgtcatcgcgccaccagggtggactggggtacggcttgagtcttcactttgcggcatcgcggctaccatcacaccccagaggggtacgctctcatcccttgacggtccgtagtttattttgctttccApoIBsaIAvaIIBsmFIHaeIIrrnB_terminator720740760780800820840860
    ctcagtcgaaagactGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACTGGATCCTTACTCGAGTCTAGACTGCAGgtcacactggctcaccttcgggtgggcctttctgcgtttatacctagggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgagagtcagctttctgaCCCGGAAAGCAAAATAGACAACAAACAGCCACTTGACCTAGGAATGAGCTCAGATCTGACGTCcagtgtgaccgagtggaagcccacccggaaagacgcaaatatggatccctatataaggcgaaggagcgagtgactgagcgatgcgagccagcaagctAhdIAvaIBmrIPstISfcITliIXbaIXhoIAvrIIBamHIBfuAIBsiEIBsoBIBspMIHpyAVPspXIPaeR7ITsp45IT7Te terminatorrrnB_terminator8809009209409609801,0001,0201,040
    ctgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacagggacgccgctcgcctttaccgaatgcttgccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgaggcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtccBtgIHgaIPfoIBsrBISacIIp15A ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    actataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtgcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttgggggAgeIEaeIBsrFIBssSIBssSαIp15A ori1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctccgcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagtacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggNspIAlwNIBpuEIBseRIBseRIBsiEIBsrFITsp45IEcoP15Ip15A ori1,4201,4401,4601,4801,5001,5201,5401,560
    tccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaatttatcaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttctagtagaataattagtctattttataaagatctaaagtcacgttaaatagAseISspIXbaIBpuEIBstBIHpyAVp15A ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcttcaaatgtagcacctgaagtcagccccatacgatataagttgttactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaatagaagtttacatcgtggacttcagtcggggtatgctatattcaacaatgatcacgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaAcuIEarISacISpeITfiIBanIIEcoRVBsiHKAIEco53kIBsp1286Ilambda t0 terminator1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatatatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataBsmIBtgIFauINcoIScaISspITatITfiIBtgZICmR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgaaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctBpmIEaeIMscIPasIBsmBIEsp3IPflMICmR2,1202,1402,1602,1802,2002,2202,2402,260
    tgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaAclIApoIBsmIFauIFokIBspEIBsrDIBtgZIBtsCICmR2,2802,3002,3202,3402,3602,3802,4002,420
    • Plasmid
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    • Linear Map
    Editing is disabled because sequence edits have been locked.
    2430 bp175020002250250500sgRNAS. pyogenes terminatorCas9 handleoverlapCmRNGS_F (DNA)NGS_R (= BC1 in Garst et al. 2017) (DNA)seq_R (DNA)

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    2430