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pTS040(dnaB)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    tatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttPvuIIBpu10Icat promotorCmR20406080100120140160
    tctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccagatatcgacgtctactagataggcgtatcacgaggcagaTCCTCTGGCGGAAAGCCTctaacatcagtgcctatgcggacatagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacggctagttgcagagtaaaagcggtctatagctgcagatgatctatccgcatagtgctccgtctAGGAGACCGCCTTTCGGAgattgtagtcacggatacgcctgtaAccIMslIZraIAatIIBsaAIBsaHIBsmBIBssSIEcoRVEsp3IBssSαIdsDNA version for cloning of sinlge mutantscat promotoroligohomology ...(A130V))sub-li...pacer180200220240260280300320340
    cgtgcgtgaacgtGTGgttGTAcgtgagatgatctcggttgcgaatgagattgccgaagcgatcTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTtcgcaaccgagatcatctcaGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGgcacgcacttgcaCACcaaCATgcactctactagagccaacgcttactctaacggcttcgctagAACTGTCGATCGAGTCAGGATCCATATTATGATCAagcgttggctctagtagagtCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCBmtINheISpeIAvrIIBsaAIAflIIIdsDNA version for cloning of sinlge mutantsoligohomology sequence (here: dnaB(A130V))sgRNACas9 handleoverlapPJ23119protospacerS. py...ator360380400420440460480500520
    TGGCACCGAGTCGGTGCTTTTTTTGAAGCTTGGGCCCGAACAAAAACTCatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacACCGTGGCTCAGCCACGAAAAAAACTTCGAACCCGGGCTTGTTTTTGAGtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgccagaggtcgaaccgacaaaaccgcctactctcttctaaaagtcggactatgtctaatttagtcttgAccIApaIBanIBpmIBsaIEarIFokIPmeISalIXmnIBaeGIBanIIBtsCIHaeIIHincIIPspOMIHindIIIBsp1286IdsDNA versi...ge mutantssgRNAS. pyogen...rminator540560580600620640660680700
    gcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactcgtcttcgccagactattttgtcttaaacggaccgccgtcatcgcgccaccagggtggactggggtacggcttgagtcttcactttgcggcatcgcggctaccatcacaccccagaggggtacgctctcatcccttgacggtccgtagtttattttgctttccgagtcagctttctgaApoIBsaIAvaIIBsmFIHaeIIrrnB_terminator720740760780800820840860880
    GGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACTGGATCCTTACTCGAGTCTAGACTGCAGgtcacactggctcaccttcgggtgggcctttctgcgtttatacctagggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatgCCCGGAAAGCAAAATAGACAACAAACAGCCACTTGACCTAGGAATGAGCTCAGATCTGACGTCcagtgtgaccgagtggaagcccacccggaaagacgcaaatatggatccctatataaggcgaaggagcgagtgactgagcgatgcgagccagcaagctgacgccgctcgcctttacAhdIAvaIBmrIPstISfcITliIXbaIXhoIAvrIIBamHIBfuAIBsiEIBsoBIBspMIBsrBIHpyAVPspXIPaeR7ITsp45IT7Te terminator9009209409609801,0001,0201,0401,060
    gcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgttcgaatgcttgccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgaggcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaBtgIHgaIPfoISacIIp15A ori1,0801,1001,1201,1401,1601,1801,2001,2201,240
    tccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtgcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaaAgeIEaeIBsiEIBsrFIBssSIBssSαIp15A ori1,2601,2801,3001,3201,3401,3601,3801,4001,420
    atccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagtacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctNspIAlwNIBpuEIBseRIBseRIBsrFITsp45IEcoP15Ip15A ori1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagcccccaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttctagtagaataattagtctattttataaagatctaaagtcacgttaaatagagaagtttacatcgtggacttcagtcggggAseIEarISspIXbaIBpuEIBstBIHpyAVp15A ori1,6201,6401,6601,6801,7001,7201,7401,7601,780
    atacgatataagttgttactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttatgctatattcaacaatgatcacgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaAcuIFauISacIScaISpeITatITfiIBanIIBtgZIEcoRVBsiHKAIEco53kIBsp1286Ilambda t0 terminatorCmR1,8001,8201,8401,8601,8801,9001,9201,940
    tgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggBsmIBtgIEaeIMscINcoIPasISspITfiICmR1,9601,9802,0002,0202,0402,0602,0802,1002,120
    agggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacatccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgtAclIBpmIBsmBIBtgZIEsp3IPflMICmR2,1402,1602,1802,2002,2202,2402,2602,2802,300
    agggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaApoIBsmIFauIFokIBspEIBsrDIBtsCICmR2,3202,3402,3602,3802,4002,420
    • Plasmid
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    • Linear Map
    Editing is disabled because sequence edits have been locked.
    2430 bp175020002250250500sgRNAS. pyogenes terminatorCas9 handleoverlapCmRNGS_F (DNA)NGS_R (= BC1 in Garst et al. 2017) (DNA)seq_R (DNA)

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    2430