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XZ105 CMV-TO-signal-hOXTR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcacggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgNdeIPvuIAsiSIRsrIIBstAPIHygromycin20406080100120140160
    tggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgaaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctBstAPIDraIIIHygromycin180200220240260280300320340
    tgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgaacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactAjuISacIIHygromycin360380400420440460480500520
    cggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcagccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtScaIHygromycin540560580600620640660680700
    aaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcattccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgtPfoIPsiISV40 pA720740760780800820840860880
    atagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacatatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtBsmIBstZ17ISV40 pA9009209409609801,0001,0201,0401,060
    caacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaagg1,0801,1001,1201,1401,1601,1801,2001,2201,240
    gcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttttcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaPciISapIBspQIpUC ori1,2601,2801,3001,3201,3401,3601,3801,4001,420
    tccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcpUC ori1,4401,4601,4801,5001,5201,5401,5601,5801,600
    cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgapUC ori1,6201,6401,6601,6801,7001,7201,7401,7601,780
    ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgcpUC ori1,8001,8201,8401,8601,8801,9001,9201,940
    gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagcaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcpUC ori1,9601,9802,0002,0202,0402,0602,0802,1002,120
    ttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttacAhdIAmp (bla)2,1402,1602,1802,2002,2202,2402,2602,2802,300
    ataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtAclIBsaIFspIAmp (bla)2,3202,3402,3602,3802,4002,4202,4402,4602,480
    ttgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttataacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataPvuIAmp (bla)2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaBcgIScaIAmp (bla)2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctAclIXmnIAmp (bla)2,8602,8802,9002,9202,9402,9602,9803,0003,020
    aatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctagggSspISgrDIbla promoterAmp (bla)3,0403,0603,0803,1003,1203,1403,1603,1803,200
    ctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcgataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgMfeI3,2203,2403,2603,2803,3003,3203,3403,3603,380
    ttcgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtaagcatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcaMluISpeIBsiWICMVCMV promoter (with TetOs)3,4003,4203,4403,4603,4803,5003,5203,5403,560
    atgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctNdeICMVCMV promoter (with TetOs)3,5803,6003,6203,6403,6603,6803,7003,720
    ctttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaagaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttCspCISnaBICMVCMV promoter (with TetOs)3,7403,7603,7803,8003,8203,8403,8603,8803,900
    tgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaacSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO3,9203,9403,9603,9804,0004,0204,0404,0604,080
    acctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcGGCgaaggggcccttgccgccaattggtctgccgtggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgCCGcttccccgggaacggcggttaaccagacggcApaIMfeIPmeIAflIIPspOMIHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SG26EG28AL30AA32NW34SA364164-6716Signal/FlagCMV promoter (with TetOs)sourcehomologhomolog4,1004,1204,1404,1604,1804,2004,2204,2404,260
    aggcggcgaacgctagtgcggccccccctggcgcggaaggtaaccgaacggcaggcccacctaggagaaatgaagctttggcgagagtggaggtggccgtcctctgccttattctgctgttggccttgtccggaaacgcttgtgtgctgctcgcgctccgcacgacccggcagaagcatccgccgcttgcgatcacgccgggggggaccgcgccttccattggcttgccgtccgggtggatcctctttacttcgaaaccgctctcacctccaccggcaggagacggaataagacgacaaccggaacaggcctttgcgaacacacgacgagcgcgaggcgtgctgggccgtcttcgtAvrIIBstEIIHindIIIEA38AN40AS42AA44PP46GA48EG50NR52TA54GP56PR58RN60EA62LA64RV66EV68AV70LC72LI74LL76LA78LS80GN82AC84VL86LA88LR90TT92RQ94KH964164-6716source4,2804,3004,3204,3404,3604,3804,4004,4204,440
    ctccagactgtttttttttatgaagcaccttagcatcgctgacctcgtcgttgcggtattccaggtgctgccccaactgctgtgggatatcacattcaggttttacggcccagacttgctctgtagacttgtcaagtacctccaggttgtcggaatgttcgcgtccacatacctccttgaggtctgacaaaaaaaaatacttcgtggaatcgtagcgactggagcagcaacgccataaggtccacgacggggttgacgacaccctatagtgtaagtccaaaatgccgggtctgaacgagacatctgaacagttcatggaggtccaacagccttacaagcgcaggtgtatggaggaaCspCIEcoRVSR98LF100FF102MK104HL106SI108AD110LV112VA114VF116QV118LP120QL122LW124DI126TF128RF130YG132PD134LL136CR138LV140KY142LQ144VV146GM148FA150ST152YL154L4164-6716source4,4604,4804,5004,5204,5404,5604,5804,6004,620
    cttttgatgtcactggaccgatgtctggcaatatgtcagccccttaggagcctgcggagaagaacggacaggctcgctgtactcgctacatggctcggctgtcttgtggcatccgcacctcaggttcatatcttttcactcagagaagtggcagatggcgtgtttgactgttgggccggaaaactacagtgacctggctacagaccgttatacagtcggggaatcctcggacgcctcttcttgcctgtccgagcgacatgagcgatgtaccgagccgacagaacaccgtaggcgtggagtccaagtatagaaaagtgagtctcttcaccgtctaccgcacaaactgacaacccggcPshAIBsu36IBsu36IL156LM158SL160DR162CL164AI166CQ168PL170RS172LR174RR176TD178RL180AV182LA184TW186LG188CL190VA192SA194PQ196VH198IF200SL202RE204VA206DG208VF210DC212WA2144164-6716source4,6404,6604,6804,7004,7204,7404,7604,7804,800
    tattcattcagccatggggacccaaggcgtatatcacctggatcacgttggctgtgtatatcgttcctgttatcgtgttggccgcttgctacggcctcatctcattcaaaatttggcagaacctccgcttgaaaacagctgccgctgctgctgcagaagctccagaaggcgcggcagcataagtaagtcggtacccctgggttccgcatatagtggacctagtgcaaccgacacatatagcaaggacaatagcacaaccggcgaacgatgccggagtagagtaagttttaaaccgtcttggaggcgaacttttgtcgacggcgacgacgacgtcttcgaggtcttccgcgccgtcgVF216IQ218PW220GP222KA224YI226TW228IT230LA232VY234IV236PV238IV240LA242AC244YG246LI248SF250KI252WQ254NL256RL258KT260AA262AA264AA266EA268PE270GA272AA2744164-6716source4,8204,8404,8604,8804,9004,9204,9404,9604,980
    tggcgacggtggacgggttgcactcgccagggtcagtagcgttaagctcatcagcaaggcaaagatccggactgttaaaatgacctttattattgtgcttgcttttattgtgtgttggactcccttcttctttgtacagatgtggagcgtgtgggacgctaacgcccccaaggaggcgaccgctgccacctgcccaacgtgagcggtcccagtcatcgcaattcgagtagtcgttccgtttctaggcctgacaattttactggaaataataacacgaacgaaaataacacacaacctgagggaagaagaaacatgtctacacctcgcacaccctgcgattgcgggggttcctccgcBsrGIGD276GG278RV280AL282AR284VS286SV288KL290IS292KA294KI296RT298VK300MT302FI304IV306LA308FI310VC312WT314PF316FF318VQ320MW322SV324WD326AN328AP330KE332A4164-6716source5,0005,0205,0405,0605,0805,1005,1205,1405,160
    agcgcgttcattatagtgatgttgctcgcgtccctgaattcctgttgtaacccgtggatctacatgctgtttactggccacctctttcacgaacttgtgcaacggtttctctgctgcagcgcttcttatctgaagggccggcgccttggcgagacgagcgcctccaagaagagcaatttcgcgcaagtaatatcactacaacgagcgcagggacttaaggacaacattgggcacctagatgtacgacaaatgaccggtggagaaagtgcttgaacacgttgccaaagagacgacgtcgcgaagaatagacttcccggccgcggaaccgctctgctcgcggaggttcttctcgttaaAfeIKasINarISapISfoIBspQIEcoRIPluTIS334AF336II338VM340LL342AS344LN346SC348CN350PW352IY354ML356FT358GH360LF362HE364LV366QR368FL370CC372SA374SY376LK378GR380RL382GE384TS386AS388KK390SN3924164-6716source5,1805,2005,2205,2405,2605,2805,3005,3205,340
    cttctagctttgtgctcagtcacaggtcctcttcccagcgcagttgttctcagccaagcacagcaGCCggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAgaagatcgaaacacgagtcagtgtccaggagaagggtcgcgtcaacaagagtcggttcgtgtcgtCGGccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTAgeIBsgIKpnIXcmIEcoNIAcc65ISS394SF396VL398SH400RS402SS404QR406SC408SQ410PS412TA414AG416GR418TP420PS422LG424PQ426DE428SC430TT432AS434SS436LA438KD440TS442SG444TG446AL448HL450DQ4524164-6716V2 tailsourcehomologhomologhADA...ain5,3605,3805,4005,4205,4405,4605,4805,500
    AACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTABstEIITP454SR456QP458IP460SE462GL464QL466HL468PQ470VL472AD474AV476SR478LV480LG482KF484GD486LT488DN490FS492SP494HA496RR498KV500LA502GV504VM506TT508GT510D4164-6716hADAR2 cat domain5,5205,5405,5605,5805,6005,6205,6405,6605,680
    GTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTBclIV512KD514AK516VI518SV520ST522GT524KC526IN528GE530YM532SD534RG536LA538LN540DC542HA544EI546IS548RR550SL552LR554FL556YT558QL560EL562YL564NN566KD568DQ5704164-6716hADAR2 cat domain5,7005,7205,7405,7605,7805,8005,8205,8405,860
    AAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCPfoIBsrGIKR572SI574FQ576KS578ER580GG582FR584LK586EN588VQ590FH592LY594IS596TS598PC600GD602AR604IF606SP608HE610PI612LE614EP616AD618RH620PN622RK624AR626GQ628LR6304164-6716hADAR2 cat domain5,8805,9005,9205,9405,9605,9806,0006,0206,040
    GACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAABcgIBlpIBamHITK632IE634SG636QG638TI640PV642RS644NA646SI648QA650WD652GV654LQ656GE658RL660LT662MS664CS666DK668IA670RW672NV674VG676IQ678GS680LL682SI684FV686EP688I4164-6716hADAR2 cat domain6,0606,0806,1006,1206,1406,1606,1806,2006,220
    TACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAvaITliIXhoIBsoBIPaeR7IY690FS692SI694IL696GS698LY700HG702DH704LS706RA708MY710QR712IS714NI716ED718LP720PL722YT724LN726KP728LL730SG732IS734NA736EA738RQ740PG742KA744PN746FS7484164-6716hADAR2 cat domain6,2406,2606,2806,3006,3206,3406,3606,3806,400
    TCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCAAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTMluIVN750WT752VG754DS756AI758EV760IN762AT764TG766KD768EL770GR772AS774RL776CK778HA780LY782CR784WM786RV788HG790KV792PS794HL796LR798SK800IT802KP804NV806YH8084164-6716hADAR2 cat domain6,4206,4406,4606,4806,5006,5206,5406,5606,580
    TGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacApaIAvaITliIXhoIBsoBIPspXIPaeR7IPspOMIES810KL812AA814KE816YQ818AA820KA822RL824FT826AF828IK830AG832LG834AW836VE838KP840TE842QD844QF846SL848TP850*4164-6716*1hADAR2 cat domainc-myc epitope6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaaBclIPmeIBGH polyadenylation sequencePolyhi...e tag6,7806,8006,8206,8406,8606,8806,9006,9206,940
    gtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctccagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagClaISphIBspDIBGH polyadenylation sequenceBGH pA6,9606,9807,0007,0207,0407,0607,0807,1007,120
    tagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtcccttatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaa7,1407,1607,1807,2007,2207,2407,2607,280
    tagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttBarIXbaIPshAIFRTHygromycin7,3007,3207,3407,3607,3807,4007,4207,4407,460
    gttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacHygromycin7,4807,5007,5207,5407,5607,5807,6007,6207,640
    cttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagagaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctEcoRIDraIIIHygromycin7,6607,6807,7007,720
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7728 bp550060006500700050010001500pUC oriNew Stop4164-67166714-6716M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)XZp023.1 (DNA)EKbF (DNA)EKo272.2 (DNA)CCp080 Cpep-F (DNA)XZp077.1 (DNA)EKo271.3 (DNA)EKo330.4 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)Hygromycin,BstAPI,AsiSI,PvuI,RsrII,NdeI,BstAPI,DraIIIAjuISacIIHyg_F (DNA),ScaIPfoISV40 pA,SV40PA_R (DNA),SV40pA-R Genewiz (DNA),PsiI,BsmIBstZ17IM13R Genewiz (DNA)BspQI,SapIpUCori5F (DNA)PciIEKo542.3 (DNA)XZp018test (DNA)BclIXZp018.1 (DNA),XZp004.3 (DNA),BsrGIPfoIhADAR2 cat domain,E488Q,T501A,EKo361.1 (DNA),+3XZp018.3 (DNA)XZp053.1 (DNA)BamHIBlpIXZp030.1 (DNA),EKo455.2 (DNA),XZp018.4 (DNA),+6MluIc-myc epitope,XZp053.2 (DNA),EKo330.5 (DNA),EKbR (DNA),+8Polyhistidine tag,cya,ToFlpR (DNA),XZp086.2 (DNA),+3BGH polyadenylation sequence,XZ41-test-R1 (DNA),BghR1 (DNA)BGH pA,bghF (DNA),SphI,BspDI,ClaIEKo293.3 (DNA),FlpInR (DNA)FRT,FrtF (DNA),EKo299.3 (DNA),XbaIEKo152.1 (DNA),BarI,PshAI,EcoRI,DraIII

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    7728