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EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF
  • Sequence Map
    Editing disabled on read-only sequence.
    agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggtt20406080100120140160
    tgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggAhdIBsaIAmp180200220240260280300320340
    agccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttctcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagAclIFspIAmp360380400420440460480500520
    attcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagataagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctAmp540560580600620640660680700
    tgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatgAclIBcgIScaIXmnIAmp720740760780800820840860
    cgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattagcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataatAmp8809009209409609801,0001,0201,040
    ttgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaat1,0601,0801,1001,1201,1401,1601,1801,2001,220
    accaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctatggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggatAloIPsiI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacctagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattgg1,4201,4401,4601,4801,5001,5201,5401,560
    accacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcactggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgFspI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatattgtctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataacaBssHIIBII(R)5'TR31...r5'...)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttgtBsiWI31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatcccagagccggcagcgtgcggggattttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtagggtctcggccgtcgcacgcccctcore insul2,1202,1402,1602,1802,2002,2202,2402,260
    cagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgacgattgagtaacactgtatctagtctacctacagggctcAAAATTGCAAGGATATATgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaactgctaactcattgtgacatagatcagatggatgtcccgagTTTTAACGTTCCTATATAMfeISfiISrfIcore insulZNF35/... site2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AAAGAGctcttcAAAATTGCAAGGATATATAAAGAGgaggtcAAAATTGCAAGGATATATAAAGAGcagaacAAAATTGCAAGGATATATAAAGAGgccttccgtatcagtctagagggtatataatggatcccatcgcgtctcagcctcactttgagctcctccacacgaattcTTTCTCgagaagTTTTAACGTTCCTATATATTTCTCctccagTTTTAACGTTCCTATATATTTCTCgtcttgTTTTAACGTTCCTATATATTTCTCcggaaggcatagtcagatctcccatatattacctagggtagcgcagagtcggagtgaaactcgaggaggtgtgcttaagXbaIZNF35/250 siteZNF35/250 siteZNF35/250 site2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cccgataccgtcgattcaaggagcttgcttgttctttttgcagaagctcagaataaacgctcaactttggcagatctaccaagcttggtaccgagctcggatccaactcgaggccaccatgccagagccagcgaagtctgctcccgccccgaaaaagggctccaagaaggcggtggggctatggcagctaagttcctcgaacgaacaagaaaaacgtcttcgagtcttatttgcgagttgaaaccgtctagatggttcgaaccatggctcgagcctaggttgagctccggtggtacggtctcggtcgcttcagacgagggcggggctttttcccgaggttcttccgccacKpnITliIXhoIPspXIAcc65IPaeR7IMP2EP4AK6SA8PA10PK12KG14SK16KA18V2744-3847MP2EP4AK6SA8PA10PK12KG14SK16KA18VH2B-GFPH2BH2B-BFPXenopus globin 5'-UTR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    actaaggcgcagaagaaaggcggcaagaagcgcaagcgcagccgcaaggagagctattccatctatgtgtacaaggttctgaagcaggtccaccctgacaccggcatttcgtccaaggccatgggcatcatgaattcgtttgtgaacgacattttcgagcgcatcgcaggtgaggtgattccgcgtcttctttccgccgttcttcgcgttcgcgtcggcgttcctctcgataaggtagatacacatgttccaagacttcgtccaggtgggactgtggccgtaaagcaggttccggtacccgtagtacttaagcaaacacttgctgtaaaagctcgcgtagcgtccactccPaqCIT20KA22QK24KG26GK28KR30KR32SR34KE36SY38SI40YV42YK44VL46KQ48VH50PD52TG54IS56SK58AM60GI62MN64SF66VN68DI70FE72RI74AG76E2744-3847T20KA22QK24KG26GK28KR30KR32SR34KE36SY38SI40YV42YK44VL46KQ48VH50PD52TG54IS56SK58AM60GI62MN64SF66VN68DI70FE72RI74AG76EH2B-GFPH2BH2B-BFP2,8202,8402,8602,8802,9002,9202,9402,960
    cttcccgcctggcgcattacaacaagcgctcgaccatcacctccagggagatccagacggccgtgcgcctgctgctgcctggggagttggccaagcacgccgtgtccgagggtactaaggccatcaccaagtacaccagcgctaaggatccccgggtaccggtcgtgtctaaggggaagggcggaccgcgtaatgttgttcgcgagctggtagtggaggtccctctaggtctgccggcacgcggacgacgacggacccctcaaccggttcgtgcggcacaggctcccatgattccggtagtggttcatgtggtcgcgattcctaggggcccatggccagcacagattcccAfeIAfeIAgeIKpnIAcc65IBpu10IA78SR80LA82HY84NK86RS88TI90TS92RE94IQ96TA98VR100LL102LP104GE106LA108KH110AV112SE114GT116KA118IT120KY122TS124AK126DP128RV130PV132VS134KG1362744-3847A78SR80LA82HY84NK86RS88TI90TS92RE94IQ96TA98VR100LL102LP104GE106LA108KH110AV112SE114GT116KA118IT120KY122TS124AK126DP128RV130PV132VS134KG136H2B-GFPH2B-BFPH2Blinkermt...22,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgaEE138LI140KE142NM144HM146KL148YM150EG152TV154DN156HH158FK160CT162SE164GE166GK168PY170EG172TQ174TM176RI178KV180VE182GG184PL186PF188AF190DI192LA1942744-3847EE138LI140KE142NM144HM146KL148YM150EG152TV154DN156HH158FK160CT162SE164GE166GK168PY170EG172TQ174TM176RI178KV180VE182GG184PL186PF188AF190DI192LA194H2B-GFPH2B-BFPmtagBFP23,1603,1803,2003,2203,2403,2603,2803,3003,320
    actagcttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgtPfoIBsu36ITS196FL198YG200SK202TF204IN206HT208QG210IP212DF214FK216QS218FP220EG222FT224WE226RV228TT230YE232DG234GV236LT238AT240QD242TS244LQ246DG248CL250IY2522744-3847TS196FL198YG200SK202TF204IN206HT208QG210IP212DF214FK216QS218FP220EG222FT224WE226RV228TT230YE232DG234GV236LT238AT240QD242TS244LQ246DG248CL250IY252H2B-GFPH2B-BFPmtagBFP23,3403,3603,3803,4003,4203,4403,4603,4803,500
    acgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagactgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctgAloINV254KI256RG258VN260FT262SN264GP266VM268QK270KT272LG274WE276AF278TE280TL282YP284AD286GG288LE290GR292ND294MA296LK298LV300GG302SH304LI306AN308AK310T2744-3847NV254KI256RG258VN260FT262SN264GP266VM268QK270KT272LG274WE276AF278TE280TL282YP284AD286GG288LE290GR292ND294MA296LK298LV300GG302SH304LI306AN308AK310TH2B-GFPH2B-BFPmtagBFP23,5203,5403,5603,5803,6003,6203,6403,660
    cacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaagcttaattaggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgttcgaattaatcT312YR314SK316KP318AK320NL322KM324PG326VY328YV330DY332RL334ER336IK338EA340NN342ET344YV346EQ348HE350VA352VA354RY356CD358LP360SK362LG364HK366LN3682744-3847*1T312YR314SK316KP318AK320NL322KM324PG326VY328YV330DY332RL334ER336IK338EA340NN342ET344YV346EQ348HE350VA352VA354RY356CD358LP360SK362LG364HK366LN368*H2B-GFPH2B-BFPmtagBFP23,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctagcgaattcgaatttaaatcggcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggatcgcttaagcttaaatttagccggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagacccBmtINheISwaIBstBIBGH polyadenylation sequenceMCS3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagcccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcNotIXmnIBGH polyadenylation sequencepSV405, 299 F4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacSexAIpSV404,2204,2404,2604,2804,3004,3204,3404,360
    cagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttttcggacttgagtggcgctgcagacagctcttcaaagactaBclIPmlIAvrIIPshAIpSV40Hygro4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattgcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaPflFITth111IHygro2, 563 F4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggtggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaAsiSIHygro4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaPflFITth111IHygro4,9204,9404,9604,9805,0005,0205,0405,060
    ccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagAjuIHygro5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgSacIIHygro3, 135 F5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatccagctgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaScaIHygroWPRE6, 730 R5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtHpaIPsiISV40 pASV40 pA(1)WPRE5,6205,6405,6605,6805,7005,7205,7405,760
    tgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacaggaacgaacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtccttgctBsaISexAI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    agtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccgggcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacgggcccgaApaISrfIEcoNIPspOMIcore insul(1) Geneious name: core insul(1)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggggggggctgtccctgatgttgacattgattattgactagttattaatagtaatccaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccccccccgacagggactacaactgtaactaataactgatcaataattatcattagSpeIcore insul(1) Geneious name: core insul(1)CMVCMV promoter (with TetOs)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataCMVCMV promoter (with TetOs)CMVCMV promoter (with TetOs)6,3206,3406,3606,3806,4006,4206,4406,460
    ttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgaaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactSnaBICMVCMV promoter (with TetOs)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacgacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgcCMVCMV promoter (with TetOs)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgcaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcBsaBIBsaBISgrDICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTTACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGCaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTCGPmeIAflIIMY2EC4NE6CG8KT10FT12RS14SN16LI18VH20QR22IH24TG26EK28PF30AC32ND34CG36KA38FT40QS42AN44LI46VH48QR50S7023-7682MY2EC4NE6CG8KT10FT12RS14SN16LI18VH20QR22IH24TG26EK28PF30AC32ND34CG36KA38FT40QS42AN44LI46VH48QR50SZNF35/ZNF250 TFZNF35/ZNF250 TFZNF35 ZF Domains 5-87,0207,0407,0607,0807,1007,1207,1407,160
    CATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGCTGTCTTGCTTGATTGTGCATCAGAGAATTCACACGGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCGACAGAACGAACTAACACGTAGTCTCTTAAGTGTGCCH52TG54EK56PY58EC60KE62CG64KA66FS68CF70SH72LI74VH76QR78IH80TA82EK84PY86DC88SE90CG92KA94FS96QL98SC100LI102VH104QR106IH108T7023-7682H52TG54EK56PY58EC60KE62CG64KA66FS68CF70SH72LI74VH76QR78IH80TA82EK84PY86DC88SE90CG92KA94FS96QL98SC100LI102VH104QR106IH108TZNF35/ZNF250 TFZNF35/ZNF250 TFZNF35 ZF Domains 5-87,1807,2007,2207,2407,2607,2807,3007,3207,340
    GCGAACGCCCCTATATGTGCGTAGAGTGCGGTAAATGTTTTGGGCGGAGTTCACATCTTTTGCAGCACCAACGTATTCACACAGGCGAGAAACCCTATGTTTGCAGCGTGTGCGGTAAGGCGTTTAGCCAGTCCTCAGTCCTTTCCAAACACCGCCGCATACATACTGGTGAGAACGCTTGCGGGGATATACACGCATCTCACGCCATTTACAAAACCCGCCTCAAGTGTAGAAAACGTCGTGGTTGCATAAGTGTGTCCGCTCTTTGGGATACAAACGTCGCACACGCCATTCCGCAAATCGGTCAGGAGTCAGGAAAGGTTTGTGGCGGCGTATGTATGACCACTCTTG110ER112PY114MC116VE118CG120KC122FG124RS126SH128LL130QH132QR134IH136TG138EK140PY142VC144SV146CG148KA150FS152QS154SV156LS158KH160RR162IH164TG166EK1687023-7682G110ER112PY114MC116VE118CG120KC122FG124RS126SH128LL130QH132QR134IH136TG138EK140PY142VC144SV146CG148KA150FS152QS154SV156LS158KH160RR162IH164TG166EK168ZNF35/ZNF250 TFZNF35/ZNF250 TFZNF250 ZF Domains 1-4Ca...r7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GCCATATGAATGTAATGAATGTGGCAAGGCTTTTAGAGTGAGTTCAGATCTCGCTCAACATCATAAGATCCATACAGGAGAGAAGCCACACGAATGTTTGGAGTGTCGAAAGGCTTTTACTCAGCTCAGTCACCTCATTCAGCATCAGAGAATTCACgctgctgcaTGGGAGCTACGGTATACTTACATTACTTACACCGTTCCGAAAATCTCACTCAAGTCTAGAGCGAGTTGTAGTATTCTAGGTATGTCCTCTCTTCGGTGTGCTTACAAACCTCACAGCTTTCCGAAAATGAGTCGAGTCAGTGGAGTAAGTCGTAGTCTCTTAAGTGcgacgacgtACCCTCGATPY170EC172NE174CG176KA178FR180VS182SD184LA186QH188HK190IH192TG194EK196PH198EC200LE202CR204KA206FT208QL210SH212LI214QH216QR218IH2207023-7682PY170EC172NE174CG176KA178FR180VS182SD184LA186QH188HK190IH192TG194EK196PH198EC200LE202CR204KA206FT208QL210SH212LI214QH216QR218IH220AA222AW224EL226ZNF35/ZNF250 TFA1A2A3W4E5L67683-7718ZNF35/ZNF250 TFZNF250 ZF Domains 1-47,5407,5607,5807,6007,6207,6407,6607,6807,700
    ggaATGtctggcggatctGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGcctTACagaccgcctagaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCBlpIBsgIPflMIEG2QS4DE6RA8LL10DQ12LH14TL16LS18NT20DA22TG24LE26EI28DR30AL32GI34PE36LV38NQ40GQ42AL44EP46KQ48DG50GW527719-8174GM228SG230GS232EG234QS236DE238RA240LL242DQ244LH246TL248LS250NT252DA254TG256LE258EI260DR262AL264GI266PE268LV270NQ272GQ274AL276EP278KQ280DG282GW284ZNF35/ZNF250 TFG7M8S9G10G11S127683-7718ZNF35/ZNF250 TFNZF DomainN (NCOA3) Activation Domain7,7207,7407,7607,7807,8007,8207,8407,860
    GATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCBmgBIEcoNIPpuMIGS54MA56EE58FV60TL62KD64VG66MD68FT70LG72DW74EQ76LG78LE80QG82DT84FW86DT88AL90DN92CQ94DL96FL98LD100PP102GG104GD106GL108GL110G7719-8174GS286MA288EE290FV292TL294KD296VG298MD300FT302LG304DW306EQ308LG310LE312QG314DT316FW318DT320AL322DN324CQ326DL328FL330LD332PP334GG336GD338GL340GL342GZNF35/ZNF250 TFZNF35/ZNF250 TFNZF DomainZ (ZNF473) Activation Domain7,8807,9007,9207,9407,9607,9808,0008,0208,040
    ACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacApaIPciITliIXhoIPspXIAflIIIPaeR7IPspOMIH112EK114FP116SD118LD120LD122MF124NG126SL128EC130DM132ES134II136RS138EL140MD142AD144GL146DF148NF150DS1527719-8174H344EK346FP348SD350LD352LD354MF356NG358SL360EC362DM364ES366II368RS370EL372MD374AD376GL378DF380NF382DS384*ZNF35/ZNF250 TF*1ZNF35/ZNF250 TFNZF DomainF (FOXO3) Activation Domainc-myc epitope8,0608,0808,1008,1208,1408,1608,1808,2008,220
    aatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgBclIPmeIBGH polyadenylation sequencePolyhi...e tag8,2408,2608,2808,3008,3208,3408,3608,3808,400
    attgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggaatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttataacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgaccccttagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatBGH polyadenylation sequence8,4208,4408,4608,4808,5008,5208,5408,560
    aatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcg8,5808,6008,6208,6408,6608,6808,7008,7208,740
    aatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccg3'TR8,7608,7808,8008,8208,8408,8608,8808,9008,920
    atggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtctacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccag8,9408,9608,9809,0009,0209,0409,0609,0809,100
    agcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcBsgIBssHII9,1209,1409,1609,1809,2009,2209,2409,260
    cttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcggaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcpLac(F)9,2809,3009,3209,3409,3609,3809,4009,4209,440
    tgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcagacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtc9,4609,4809,5009,5209,5409,5609,5809,6009,620
    gaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcPciIAflIII9,6409,6609,6809,7009,7209,7409,7609,7809,800
    ctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcg9,8209,8409,8609,8809,9009,9209,9409,960
    tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccataga9,98010,00010,02010,04010,06010,08010,10010,12010,140
    gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttct10,16010,18010,20010,22010,24010,26010,280
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10283 bp75008000850090009500100005001000150020002500ZNF35/ZNF250 TF7719-81748175-81777683-7718EWo137.2 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),AhdI,BsaI,FspI,AclIAmp,ScaI,BcgIAclIXmnIPsiI,AloIFrtF (DNA)FlpInR (DNA)M13-40FOR Genewiz (DNA),FspIBII(R),M13F Genewiz (DNA),BssHII5'TR31bp spacer,5'TR(1),5'TR,5'TR,EW_PB_F (DNA),BsiWIYX021-seq-3 (DNA)core insul,EWXglobF NONSPECIFIC DONT USE (DNA),SrfIAdded MluI site,Gibson overlap,MluI-PGK-GF,+6ZNF35/250 siteZNF35/250 site,ZNF35/250 site,EWo137.1 (DNA),XbaIEWo196.1 (DNA),EWo196.2 (DNA)N (NCOA3) Activation Domain,BsgI,BlpIEW_NZF_R (DNA),PflMI,BmgBINZF Domain,Z (ZNF473) Activation Domain,EcoNI,PpuMIF (FOXO3) Activation Domain,AflIII,PciIc-myc epitope,New Stop,Polyhistidine tag,cya,+10BGH polyadenylation sequence,BGHR Genewiz (DNA),+1bghF (DNA)EWo370.2 (DNA)EW_PB_R (DNA)3'TRBsgI,BssHIIpLac(F),M13R Genewiz (DNA)pUCori5F (DNA)pUCoriR (DNA),AflIII,PciI

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    10283