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EW369 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcCMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoICspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAABbsIPmeIAflIICspCIHindIIIMY2EC4NE6CG8KT10FT12RS14SN16LI18VH20QR22IH24TG26EK28PF30AC32ND34CG36KA38F760-1419ZNF35 ZF Domains 5-8CMV promot...th TetOs)720740760780800820840860
    TACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCStuIStuIBsrGIT40QS42AN44LI46VH48QR50SH52TG54EK56PY58EC60KE62CG64KA66FS68CF70SH72LI74VH76QR78IH80TA82EK84PY86DC88SE90CG92KA94FS96Q760-1419ZNF35 ZF Domains 5-88809009209409609801,0001,0201,040
    CTGTCTTGCTTGATTGTGCATCAGAGAATTCACACGGGCGAACGCCCCTATATGTGCGTAGAGTGCGGTAAATGTTTTGGGCGGAGTTCACATCTTTTGCAGCACCAACGTATTCACACAGGCGAGAAACCCTATGTTTGCAGCGTGTGCGGTAAGGCGTTTAGCCAGTCCTCAGGACAGAACGAACTAACACGTAGTCTCTTAAGTGTGCCCGCTTGCGGGGATATACACGCATCTCACGCCATTTACAAAACCCGCCTCAAGTGTAGAAAACGTCGTGGTTGCATAAGTGTGTCCGCTCTTTGGGATACAAACGTCGCACACGCCATTCCGCAAATCGGTCAGGAGTCL98SC100LI102VH104QR106IH108TG110ER112PY114MC116VE118CG120KC122FG124RS126SH128LL130QH132QR134IH136TG138EK140PY142VC144SV146CG148KA150FS152QS154S760-1419ZNF250 ZF Domains 1-4ZNF35 ZF Domains 5-8Cano...ker1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TCCTTTCCAAACACCGCCGCATACATACTGGTGAGAAGCCATATGAATGTAATGAATGTGGCAAGGCTTTTAGAGTGAGTTCAGATCTCGCTCAACATCATAAGATCCATACAGGAGAGAAGCCACACGAATGTTTGGAGTGTCGAAAGGCTTTTACTCAGCTCAGTCACCTCATAGGAAAGGTTTGTGGCGGCGTATGTATGACCACTCTTCGGTATACTTACATTACTTACACCGTTCCGAAAATCTCACTCAAGTCTAGAGCGAGTTGTAGTATTCTAGGTATGTCCTCTCTTCGGTGTGCTTACAAACCTCACAGCTTTCCGAAAATGAGTCGAGTCAGTGGAGTAV156LS158KH160RR162IH164TG166EK168PY170EC172NE174CG176KA178FR180VS182SD184LA186QH188HK190IH192TG194EK196PH198EC200LE202CR204KA206FT208QL210SH212LI214760-1419ZNF250 ZF Domains 1-41,2401,2601,2801,3001,3201,3401,3601,3801,400
    TCAGCATCAGAGAATTCACgctgctgcaTGGGAGCTAggaATGtctggcggatctGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGAGTCGTAGTCTCTTAAGTGcgacgacgtACCCTCGATcctTACagaccgcctagaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCAvaIBlpIBsgIPasIBsoBIBstXIQH216QR218IH220760-1419A1A2A3W4E5L6G7M8S9G10G11S121420-1455EG2QS4DE6RA8LL10DQ12LH14TL16LS18NT20DA22TG24LE26EI28DR30AL32GI34PE36LV38NQ401456-1911N (NCOA3) Activation DomainNZF DomainZNF250...s 1-41,4201,4401,4601,4801,5001,5201,5401,560
    GGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGPasIBmgBIEcoNIPflMIPpuMIGQ42AL44EP46KQ48DG50GW52GS54MA56EE58FV60TL62KD64VG66MD68FT70LG72DW74EQ76LG78LE80QG82DT84FW86DT88AL90DN92CQ94DL96FL981456-1911NZF DomainZ (ZNF473) Activation DomainN (NCOA3) A...ion Domain1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaggccgctcgaggACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatccggcgagctccAvaIPciITliIXhoIBsoBIBstXIPspXIPaeR7I*1LD100PP102GG104GD106GL108GL110GH112EK114FP116SD118LD120LD122MF124NG126SL128EC130DM132ES134II136RS138EL140MD142AD144GL146DF148NF150DS1521456-1911NZF DomainF (FOXO3) Activation DomainZ ...n1,7601,7801,8001,8201,8401,8601,8801,9001,920
    agggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactccctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggApaIBclIPmeIPspOMIBGH polyadenylation sequencec-myc epitopePolyhi...e tagEWf101,9401,9601,9802,0002,0202,0402,0602,0802,100
    actgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccBbsIClaISphIBspDIBGH polyadenylation sequenceBGH pA2,1202,1402,1602,1802,2002,2202,2402,260
    tgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcgBGH pA2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccgtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggMscINaeIXbaINgoMIVFRT2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttcBarIBsaXIPflFIPshAITth111IHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccgPstIBfuAIBspMIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    catggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtcNcoIPvuIAsiSIPflFIRsrIITth111IHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctcgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagaDraIIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacAjuISacIIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggScaIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    gccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaNaeIPfoIPsiINgoMIVSV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacattBstZ17ISV40 pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    agcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgSapIBsaXIBspQI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgcPciIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagatgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaapUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttaBspHIpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctAhdIBsaIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctactgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatAclIFspIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    caggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgPvuIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatttcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaBcgIScaIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgAclIXmnIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctccccctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggSspIBspHISgrDIbla promoterAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaMfeIBpu10I6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttgcgctgcttcgtacgggccagatatacgcaacgcgacgaagcatgcccggtctatatgcgMluIBsiWI6,320
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6331 bp450050005500600050010001500NZF DomainTetO760-14191420-14551456-1911EWo196.1 (DNA)FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)

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    6331