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pZCS16 (eft-3p::wrmScarlet::tbb-2UTR in pUC19)
  • Sequence Map
    Editing disabled on read-only sequence.
    atctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgori20406080100120140160
    tctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccAcuIMmeIAlwNIori180200220240260280300320340
    ttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaaaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattMmeIori360380400420440460480500520
    gcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaBciVIoripBR322ori-F540560580600620640660680700
    ggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctcccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagagPciISapIBspQIAflIII720740760780800820840860
    cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcgggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagPvuIIlac promoterCAP binding sitelac operator8809009209409609801,0001,0201,040
    acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcgactctagaggatcccGCACCTTTGGTCTTTTATTGTCAACTTCCATTGGTTCTTCCATTGTTTCTGTTAAATTAATGAATTTTTCATAAAATAAAGACATTATACAATATAAAAATGAAGAtgtgtcctttgtcgatactggtactaatgcggttcgaacgtacggacgtccagctgagatctcctagggCGTGGAAACCAGAAAATAACAGTTGAAGGTAACCAAGAAGGTAACAAAGACAATTTAATTACTTAAAAAGTATTTTATTTCTGTAATATGTTATATTTTTACTTCTAccIPstISalISbfISphIXbaIBfuAIBspMIHindIIIpEFT-3 promoterM13 ReverseM13 rev1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ATTTATTGAAAATAAACTGCCAGAGAGAAAAAGTATGCAACACTCCCGCCGAGAGTGTTTGAAATGGTGTACGGTACATTTTCGTGCTAGGAGTTAGATGTGCAGGCAGCAACGAGAGGGGGAGAGATTTTTTTGGGCCTTGTGAAATTAACGTGAGTTTTCTGGTCATCTGACTTAAATAACTTTTATTTGACGGTCTCTCTTTTTCATACGTTGTGAGGGCGGCTCTCACAAACTTTACCACATGCCATGTAAAAGCACGATCCTCAATCTACACGTCCGTCGTTGCTCTCCCCCTCTCTAAAAAAACCCGGAACACTTTAATTGCACTCAAAAGACCAGTAGACTGABsgINmeAIIIpEFT-3 promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AATCATGTTGGTTTTTTGTTGGTTTATTTTGTTTTTATCTTTGTTTTTATCCAGATTAGGAAATTTAAATTTTATGAATTTATAATGAGGTCAAACATTCAGTCCCAGCGTTTTTCCTGTTCTCACTGTTTAGTCGAATTTTTATTTTAGGCTTTCAACAAATGTTCTAACTGTCTTAGTACAACCAAAAAACAACCAAATAAAACAAAAATAGAAACAAAAATAGGTCTAATCCTTTAAATTTAAAATACTTAAATATTACTCCAGTTTGTAAGTCAGGGTCGCAAAAAGGACAAGAGTGACAAATCAGCTTAAAAATAAAATCCGAAAGTTGTTTACAAGATTGACAGPsiISwaIpEFT-3 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    TTATTTGTGACCTCACTTTTTATATTTTTTTAATTTTTAAAAATATTAGAAGTTTCTAGGATAATTTTTTCGACTTTTATTCTCTCTACCGTCCGCACTCTTCTTACTTTTAAATTAAATTGTTTTTTTTTCAGTTGGGAAACACTTTGCTCTAGAaaaaATGGTCAGCAAGGGAAATAAACACTGGAGTGAAAAATATAAAAAAATTAAAAATTTTTATAATCTTCAAAGATCCTATTAAAAAAGCTGAAAATAAGAGAGATGGCAGGCGTGAGAAGAATGAAAATTTAATTTAACAAAAAAAAAGTCAACCCTTTGTGAAACGAGATCTttttTACCAGTCGTTCCCTXbaIMV2SK4G1736-2434pEFT-3 promoterwrmScarlet1,5801,6001,6201,6401,6601,6801,7001,7201,740
    GAGGCAGTTATCAAGGAGTTCATGCGTTTCAAGGTCCACATGGAGGGATCCATGAACGGACACGAGTTCGAGATCGAGGGAGAGGGAGAGGGACGTCCATACGAGGGAACCCAAACCGCCAAGCTCAAGGTCACCAAGGGAGGACCACTCCCATTCTCCTGGGACATCCTCTCCCCTCCGTCAATAGTTCCTCAAGTACGCAAAGTTCCAGGTGTACCTCCCTAGGTACTTGCCTGTGCTCAAGCTCTAGCTCCCTCTCCCTCTCCCTGCAGGTATGCTCCCTTGGGTTTGGCGGTTCGAGTTCCAGTGGTTCCCTCCTGGTGAGGGTAAGAGGACCCTGTAGGAGAGGGBplIBplIStyIBstXIBstEIIE6AV8IK10EF12MR14FK16VH18ME20GS22MN24GH26EF28EI30EG32EG34EG36RP38YE40GT42QT44AK46LK48VT50KG52GP54LP56FS58WD60IL62S1736-2434wrmScarlet1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CACAATTCATGTACGGATCCCGTGCCTTCACCAAGCACCCAGCCGACATCCCAGACTACTACAAGCAATCCTTCCCAGAGGGATTCAAGTGGGAGCGTGTCATGAACTTCGAGGACGGAGGAGCCGTCACCGTCACCCAAGACACCTCCCTCGAGGACGGAACCCTCATCTACAAGTGTTAAGTACATGCCTAGGGCACGGAAGTGGTTCGTGGGTCGGCTGTAGGGTCTGATGATGTTCGTTAGGAAGGGTCTCCCTAAGTTCACCCTCGCACAGTACTTGAAGCTCCTGCCTCCTCGGCAGTGGCAGTGGGTTCTGTGGAGGGAGCTCCTGCCTTGGGAGTAGATGTTAvaITliIXhoIBseRIBsoBICspCIPspXIPaeR7IP64QF66MY68GS70RA72FT74KH76PA78DI80PD82YY84KQ86SF88PE90GF92KW94ER96VM98NF100ED102GG104AV106TV108TQ110DT112SL114ED116GT118LI120YK1221736-2434wrmScarlet1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GGTCAAGCTCCGTGGAACCAACTTCCCACCAGACGGACCAGTCATGCAAAAGAAGACCATGGGATGGGAGGCCTCCACCGAGCGTCTCTACCCAGAGGACGGAGTCCTCAAGGGAGACATCAAGATGGCCCTCCGTCTCAAGGACGGAGGACGTTACCTCGCCGACTTCAAGACCCCAGTTCGAGGCACCTTGGTTGAAGGGTGGTCTGCCTGGTCAGTACGTTTTCTTCTGGTACCCTACCCTCCGGAGGTGGCTCGCAGAGATGGGTCTCCTGCCTCAGGAGTTCCCTCTGTAGTTCTACCGGGAGGCAGAGTTCCTGCCTCCTGCAATGGAGCGGCTGAAGTTCTGGBbsIBtgIBtgINcoIStuIStyIPflFIPflMITth111IVK124LR126GT128NF130PP132DG134PV136MQ138KK140TM142GW144EA146ST148ER150LY152PE154DG156VL158KG160DI162KM164AL166RL168KD170GG172RY174LA176DF178KT1801736-2434wrmScarlet2,1202,1402,1602,1802,2002,2202,2402,260
    ACCTACAAGGCCAAGAAGCCAGTCCAAATGCCAGGAGCCTACAACGTCGACCGTAAGCTCGACATCACCTCCCACAACGAGGACTACACCGTCGTCGAGCAATACGAGCGTTCCGAGGGACGTCACTCCACCGGAGGAATGGACGAGCTCTACAAGTAAACTAGTgataaatgcaTGGATGTTCCGGTTCTTCGGTCAGGTTTACGGTCCTCGGATGTTGCAGCTGGCATTCGAGCTGTAGTGGAGGGTGTTGCTCCTGATGTGGCAGCAGCTCGTTATGCTCGCAAGGCTCCCTGCAGTGAGGTGGCCTCCTTACCTGCTCGAGATGTTCATTTGATCActatttacgtAccISacISalISpeIXcmIBanIIEco53kITY182KA184KK186PV188QM190PG192AY194NV196DR198KL200DI202TS204HN206ED208YT210VV212EQ214YE216RS218EG220RH222ST224GG226MD228EL230YK232*1736-2434wrmScarlettb...R2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaatcctttcaagcattcccttcttctctatcactcttctttctttttgtcaaaaaattctctcgctaatttatttgcttttttaatgttattattttatgactttttatagtcactgaaaagtttgcatctgagtgaagtgaatgctatcaaaatgtgattctgtctgatgtactttaggaaagttcgtaagggaagaagagatagtgagaagaaagaaaaacagttttttaagagagcgattaaataaacgaaaaaattacaataataaaatactgaaaaatatcagtgacttttcaaacgtagactcacttcacttacgatagttttacactaagacagactacatgBsmIBsmItbb-2 3'UTR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tttcacaatctctcttcaattccattttgaagtgctttaaacccgaaaggttgagaaaaatgcgagcgctcaaatatttgtattgtgttcgttgagtgacccaacaaaaagaggaaactttattgtgccgccaagaaaaaagtctcacgggtaccgagctcgaattcactggccgaaagtgttagagagaagttaaggtaaaacttcacgaaatttgggctttccaactctttttacgctcgcgagtttataaacataacacaagcaactcactgggttgtttttctcctttgaaataacacggcggttcttttttcagagtgcccatggctcgagcttaagtgaccggcAfeIKpnISacIBanIIEcoRIAcc65IEco53kItbb-2 3'UTR2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgBglIBmrIFspIKasINarIPvuISfoIPluTIPvuII2,8202,8402,8602,8802,9002,9202,9402,960
    gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacNdeIPfoIBstAPIpRS-markerpGEX 3'2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaataacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatEcoO109IAmpR promoterpBRforEco3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaaBciVIAmpRAmpR promoter3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagaAclIAcuIBcgIXmnIAmpRAmp-R3,5203,5403,5603,5803,6003,6203,6403,660
    cagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatggggggtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtacccccPvuIScaIAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    atcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataatagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctattAclIFspIBsrDIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    agttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgatcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctAhdIBglIBmrIBpmIBsaIBsrDIBsrFINmeAIIIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgatattatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactatAmpR4,2204,2404,2604,2804,3004,320
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4339 bp32503500375040002505007501000M13 ReverseM13 revAmpRAmp-RoriZCS231 (DNA)ZCS241 (DNA)ZCS249 (DNA)ZCS238 (DNA)ZCS271 (DNA)MS218 (DNA)MS245 (DNA)MS785 (DNA)ZCS157 (DNA)ZCS235 (DNA)ZCS237 (DNA)ZCS150 (DNA)MS120 (DNA)M13F (DNA)MS595 (DNA)

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    4339