Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
HR130PA_DTA_V5HAFLAG_Narms
  • Sequence Map
    Editing disabled on read-only sequence.
    TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCSalIEcoRI fragment20406080100120140160
    AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTATCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATFspINdeIBstAPISalIEcoRI fragment180200220240260280300320340
    CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGNNNNNGAATTCGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGAAATTCTACCCATACGATGTTCCAGATTACGCTGACTACAAAGACGATGACGACAAGAAATTCgctgagggcagaggGCGGTCCCAAAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACNNNNNCTTAAGCCATTCGGATAGGGATTGGGAGAGGAGCCAGAGCTAAGATGCTTTAAGATGGGTATGCTACAAGGTCTAATGCGACTGATGTTTCTGCTACTGCTGTTCTTTAAGcgactcccgtctccEcoRIE1G2R3G4515-568G1K2P3I4P5N6P7L8L9G10L11D12S13T14407-448D1Y2K3D4D5D6D7K8482-505Y1P2Y3D4V5P6D7Y8A9455-481bamhI ecoRI fragmentEcoRI fusion siteV5_FOR_HR130DTA_FWDV5_FOR_HR130DTA_REVEcoRI fusion siteV5_FOR_HR130DTA_REVV5_FOR_HR130DTA_FWDV5HAFLAGV5p1_pcr_L2A...eSalIEcoRI fragment360380400420440460480500520
    aagtcttctaacatgcggtgacgtggaggagaatcccggcccttccggaatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccttcagaagattgtacgccactgcacctcctcttagggccgggaaggccttacctctcgctgctctcgccggacgggcggtacctctagctcacggcgtagtggccgtgggacttgccgcacctcaagctcgaccacccgccgcctctcccgtgggggttcgtcccggcgtactggS5L6L7T8C9G10D11V12E13E14N15P16G17P18515-568bamhI ecoRI fragmentGFP2A\Peptide114_HR..._seqR540560580600620640660680700
    aacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacgttgttctacttctcgtggtttccgcgggactggaagtcggggatggacgactcggtgcactacccgatgccgaagatggtgaagccgtggatggggtcgccgatgctcttggggaaggacgtgcggtagttgttgccgccgatgtggttgtgggcgtagctcttcatgctcctgcPmlISapIXmnIBspQIbamhI ecoRI fragmentGFP720740760780800820840860
    gcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcgccgcacgacgtgcactcgaagtcgatggcgatgctccggccggcgcactagccgctgaagttccaccacccgtggccgaaggggctcctgtcgcactagaagtggctgttctagtaggcgtcgttgcggtggcacctcgtggacgtggggtacccgctattgcacgaccacccFseIPmlIPaqCIbamhI ecoRI fragmentGFP8809009209409609801,0001,0201,040
    cagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggaggtcgaagcgggcgtggaagtcggacgcgctgccgccgatgatgtcgaagcaccacctgtcggtgtacgtgaagttctcgcggtaggtggggtcgtaggacgtcttgcccccggggtacaagcggaaggcggcgcacctcctcgacgtgtcgttgtggctcgacccgtagcacctcApaIPspOMIbamhI ecoRI fragmentGFP1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgctagagctgaatctaagtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaatggtcgtgcggaagttctgggggtagcggaagcggtctagggcgcgagtcagcaggttaagacggcacctgccgtggcggcctgggccgaggtggcctagagcgatctcgacttagattcagctgttagttggagacctaatgttttaaacactttctaactgaccataagaatAccISalIXcmIbamhI ecoRI fragmentGFPWPRE1,2401,2601,2801,3001,3201,3401,3601,3801,400
    actatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgbamhI ecoRI fragmentWPRE1,4201,4401,4601,4801,5001,5201,5401,560
    tgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcaactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtbamhI ecoRI fragmentWPRE1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttagcaggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaabamhI ecoRI fragmentWPRE1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gggccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaaataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggcccggcggaggggcggaccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggttgcttttattctagacgaaaaacgaacatgacccagagagaccaatctggtctagactcggacccKpnIAcc65IbamhI ecoRI fragment3'dLTRWPRE1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcatcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtAflIIbamhI ecoRI fragment3'dLTR2,1202,1402,1602,1802,2002,2202,2402,260
    gtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcataaatattgaacgtttctttacttatagtctctcactctccttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccbamhI ecoRI fragmentSV40\EEL\poly(A)s2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctctagctatcccgcccctaactAAAATTCGAGCTCGGTACCTCGCGAATGCATCAGATCTTTCCactcAAAACatATAACTTCGTATAgcatacatTATACGAAGTTATatTCAAAAATCTAGAcaGCGGCCGCCaCTCAAAATTTCTTCTATAAAGTAACAAAACTTTTATGAgagatcgatagggcggggattgaTTTTAAGCTCGAGCCATGGAGCGCTTACGTAGTCTAGAAAGGtgagTTTTGtaTATTGAAGCATATcgtatgtaATATGCTTCAATAtaAGTTTTTAGATCTgtCGCCGGCGGtGAGTTTTAAAGAAGATATTTCATTGTTTTGAAAATACTKpnINotINruINsiIXbaIAcc65IbamhI ecoRI fragment>LoxPSV40\Ori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTCCCTGTCGGGGGGGGGTTTCGGGGGTCCCTACATTAATGCAGGGAGGGGGCGATCCCCCGTCGTCGCTCGGCGGGCCCCGAGGCGAGGCCAGGCCGCGAGGGGGGCGTAGGGGCTCGGCCGTCGCACGCCCCTGTCGGGCCCGTGCCCCTTCCACCGTGCCCTAGCGAAAGGAGASrfIbamhI ecoRI fragmentcore insul2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAGGCCTCCAAGGCCttaaTTCGgccgcaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgCTTGCGAAGAGCGACGAGAAACTCGGACGTCTGTGGACCCCCCTATGCCCCTTTTCCGGAGGTTCCGGaattAAGCcggcgttcctagacgctagcgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttgcMfeISfiIAsiSIbamhI ecoRI fragmentEF1 promotercore insul2,8202,8402,8602,8802,9002,9202,9402,960
    ggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtcgacttcgaagctccccgagcgtagabamhI ecoRI fragmentEF1 promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcaggaggaagtgcgcgggcggcgggatggactccggcggtaggtgcggccaactcagcgcaagacggcggagggcggacaccacggaggacttgacgcaggcggcagatccattcaaatttcgagtccagctctggcccggaaacaggccgcgagggaacctcggatggatctgagtcCspCIBsu36IbamhI ecoRI fragmentEF1 promoter3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgatatcgccaccatggccagctccgaggatgtcatcaaagagtttatgagatttaaggtcaagatggagggaaggccgagaggtgcgaaacggactgggacgaacgagttgagatgcagaaacaaagcaaaagacaagacgcggcaatgtctaggttcgacactggccgcggatgctatagcggtggtaccggtcgaggctcctacagtagtttctcaaatactctaaattccagttctacctcccttEcoRVbamhI ecoRI fragmentEF1 promotermRuby3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gcgtcaacggacacgagttcgagattgagggagaaggagaaggccggccttacgagggcacacaaaccgctaagctcaaggtcacaaaaggaggacccctccccttctcctgggatattctgagccctcagttccagtacggaagcaaagcctatgttaaacaccctgccgacatcgcagttgcctgtgctcaagctctaactccctcttcctcttccggccggaatgctcccgtgtgtttggcgattcgagttccagtgttttcctcctggggaggggaagaggaccctataagactcgggagtcaaggtcatgccttcgtttcggatacaatttgtgggacggctgtaBplIBplIFseIbamhI ecoRI fragmentmRuby3,5203,5403,5603,5803,6003,6203,6403,660
    ccctgactatctgaagctctccttccctgaaggcttcaagtgggagagattcatgaacttcgaggacggaggcgtggtgacagtcacacaagatagcaccctccaggacggagagtttatttataaggtgaaactcagaggaaccaacttcccctccgatggccctgtcatgcaagggactgatagacttcgagaggaagggacttccgaagttcaccctctctaagtacttgaagctcctgcctccgcaccactgtcagtgtgttctatcgtgggaggtcctgcctctcaaataaatattccactttgagtctccttggttgaaggggaggctaccgggacagtacgttbamhI ecoRI fragmentmRuby3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aaaaaaacaatgggatgggaagcctccaccgagagaatgtatcctgaggatggcgctctgaaaggcgaaattaaaatgagactgaaactcaaagacggaggacactacgatgccgaggtcaaaacaacctacaaggccaagaaacaagtgcagctgcctggcgcctacatgactgtttttttgttaccctacccttcggaggtggctctcttacataggactcctaccgcgagactttccgctttaattttactctgactttgagtttctgcctcctgtgatgctacggctccagttttgttggatgttccggttctttgttcacgtcgacggaccgcggatgtactgacBsu36IbamhI ecoRI fragmentmRuby3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atattaaactcgacattatcagccataatggggactacaccatcgtggaacaatatgagagagctgagggcagacatagcacaggcgctggaGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTatgaccgagtacaagcccacggtgcgccttataatttgagctgtaatagtcggtattacccctgatgtggtagcaccttgttatactctctcgactcccgtctgtatcgtgtccgcgacctCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCCGGGAtactggctcatgttcgggtgccacgcggaAleIbamhI ecoRI fragmentmRubyT2APuro4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgcggtgggcgctgctgcaggggtcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgBsiWIPflFIBstEIITth111IbamhI ecoRI fragmentPuro4,2204,2404,2604,2804,3004,3204,3404,360
    gacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccactgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtbamhI ecoRI fragmentPuro4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgccggcagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacggBcgIBssHIIbamhI ecoRI fragmentPuro4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggaCGAAAttCCGCGGttattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcaGTTAACTTGgcttcctggcgcgtggaccacgtactgggcgttcgggccacggactttagttggagacctGCTTTaaGGCGCCaataatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtCAATTGAACHpaIBstAPIDraIIIbamhI ecoRI fragmentPuro4,7404,7604,7804,8004,8204,8404,8604,8804,900
    TTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGAATTGACTCAAATGATGTCAATTAGTCTATCAGAAGCTATCTGGTCTCCAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCTTAACTGAGTTTACTACAGTTAATCAGATAGTCTTCGATAGACCAGAGGbamhI ecoRI fragmentSV40 pA4,9204,9404,9604,9805,0005,0205,0405,060
    CTTCCGGGGGACAAGACATCCCTGTTTAATATTTAAACAGCAGTGTTCCCAAACTGGGTTCTTATATCCCTTGCTCTGGTCAACCAGGTTGCAGGGTTTCCTGTCCTCACAGGAACGAAGTCCCTAAAGAAACAGTGGCAGCCAGGTTTAGCCCCGGAATTGACTGGATTCCTTTGAAGGCCCCCTGTTCTGTAGGGACAAATTATAAATTTGTCGTCACAAGGGTTTGACCCAAGAATATAGGGAACGAGACCAGTTGGTCCAACGTCCCAAAGGACAGGAGTGTCCTTGCTTCAGGGATTTCTTTGTCACCGTCGGTCCAAATCGGGGCCTTAACTGACCTAAGGAAASspIbamhI ecoRI fragment5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TTTAGGGCCCATTGGTATGGCTTTTTCCCCGTATCCCCCCAGGTGTCTGCAGGCTCAAAGAGCAGCGAGAAGCGTTCAGAGGAAAGCGATCCCGTGCCACCTTCCCCGTGCCCGGGCTGTCCCCGCACGCTGCCGGCTCGGGGATGCGGGGGGAGCGCCGGACCGGAGCGGAGCCAAATCCCGGGTAACCATACCGAAAAAGGGGCATAGGGGGGTCCACAGACGTCCGAGTTTCTCGTCGCTCTTCGCAAGTCTCCTTTCGCTAGGGCACGGTGGAAGGGGCACGGGCCCGACAGGGGCGTGCGACGGCCGAGCCCCTACGCCCCCCTCGCGGCCTGGCCTCGCCTCGGApaISrfIBstXIPspOMIbamhI ecoRI fragmentcore insul5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CCGGGCGGCTCGCTGCTGCCCCCTAGCGGGGGAGGGACGTAATTACATCCCTGGGGGCTTTGGGGGGGGGCTGTCCCTGATATCAAACatATAACTTCGTATAgcatacatTATACGAAGTTATtTTATCACGTAAGTAGAACATGAAAtaacCTAGATCGGATCCNNNNNTCGAGGCCCGCCGAGCGACGACGGGGGATCGCCCCCTCCCTGCATTAATGTAGGGACCCCCGAAACCCCCCCCCGACAGGGACTATAGTTTGtaTATTGAAGCATATcgtatgtaATATGCTTCAATAaAATAGTGCATTCATCTTGTACTTTattgGATCTAGCCTAGGNNNNNAGCTBamHIEcoRVbamhI ecoRI fragmentcore insul>LoxP5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CTGCATGCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTGACGTACGCCCATCCCCTCCGCGAAAAGGGTTCCGTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAGCGTGTGTAAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGGAAGATGAAgeIsource:PGKdtabpAMSCV_rev_primerprimer...0/130SalIEcoRI fragment5,6205,6405,6605,6805,7005,7205,7405,760
    CCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTGTCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGACCCSpeIME2AG4RP6LG8QR10PI12AA14LL16LR18FL20G5889-6782source:PGKdtabpAORF frame 1SalIEcoRI fragment5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGCAGGTCCTCGCCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCGAAGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCTGGACGTCCAGGAGCGGSbfIS22EA24GK26GW28VR30GR32AQ34GR36AQ38GR40GG42RP44KV46LR48RP50GI52LH54AS56KA58HV60CR62AV64LL66FL68IS70GP72FD74LQ76VL78A5889-6782source:PGKdtabpAORF frame 1possibly deleted in all plasmidsmPGK_F_primerSalIEcoRI fragment5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ATGGATCCTGATGATGTTGTTGATTCTTCTAAATCTTTTGTGATGGAAAACTTTTCTTCGTACCACGGGACTAAACCTGGTTATGTAGATTCCATTCAAAAAGGTATACAAAAGCCAAAATCTGGTACACAAGGAAATTATGACGATGATTGGAAAGGGTTTTATAGTACCGACATACCTAGGACTACTACAACAACTAAGAAGATTTAGAAAACACTACCTTTTGAAAAGAAGCATGGTGCCCTGATTTGGACCAATACATCTAAGGTAAGTTTTTCCATATGTTTTCGGTTTTAGACCATGTGTTCCTTTAATACTGCTACTAACCTTTCCCAAAATATCATGGCTGTAccIBamHIBstZ17IM80DP82DD84VV86DS88SK90SF92VM94EN96FS98SY100HG102TK104PG106YV108DS110IQ112KG114IQ116KP118KS120GT122QG124NY126DD128DW130KG132FY134ST136D5889-6782source:PGKdtabpAORF frame 1SalIEcoRI fragment6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ATAAATACGACGCTGCGGGATACTCTGTAGATAATGAAAACCCGCTCTCTGGAAAAGCTGGAGGCGTGGTCAAAGTGACGTATCCAGGACTGACGAAGGTTCTCGCACTAAAAGTGGATAATGCCGAAACTATTAAGAAAGAGTTAGGTTTAAGTCTCACTGAACCGTTGATGGATATTTATGCTGCGACGCCCTATGAGACATCTATTACTTTTGGGCGAGAGACCTTTTCGACCTCCGCACCAGTTTCACTGCATAGGTCCTGACTGCTTCCAAGAGCGTGATTTTCACCTATTACGGCTTTGATAATTCTTTCTCAATCCAAATTCAGAGTGACTTGGCAACTACCTN138KY140DA142AG144YS146VD148NE150NP152LS154GK156AG158GV160VK162VT164YP166GL168TK170VL172AL174KV176DN178AE180TI182KK184EL186GL188SL190TE192PL194ME1965889-6782source:PGKdtabpAORF frame 1SalIEcoRI fragment6,3206,3406,3606,3806,4006,4206,4406,460
    GCAAGTCGGAACGGAAGAGTTTATCAAAAGGTTCGGTGATGGTGCTTCGCGTGTAGTGCTCAGCCTTCCCTTCGCTGAGGGGAGTTCTAGCGTTGAATATATTAATAACTGGGAACAGGCGAAAGCGTTAAGCGTAGAACTTGAGATTAATTTTGAAACCCGTGGAAAACGTGGCCGTTCAGCCTTGCCTTCTCAAATAGTTTTCCAAGCCACTACCACGAAGCGCACATCACGAGTCGGAAGGGAAGCGACTCCCCTCAAGATCGCAACTTATATAATTATTGACCCTTGTCCGCTTTCGCAATTCGCATCTTGAACTCTAATTAAAACTTTGGGCACCTTTTGCACCGQV198GT200EE202FI204KR206FG208DG210AS212RV214VL216SL218PF220AE222GS224SS226VE228YI230NN232WE234QA236KA238LS240VE242LE244IN246FE248TR250GK252RG2545889-6782source:PGKdtabpAORF frame 1SalIEcoRI fragment6,4806,5006,5206,5406,5606,5806,6006,6206,640
    CAAGATGCGATGTATGAGTATATGGCTCAAGCCTGTGCAGGAAATCGTGTCAGGCGATCTCTTTGTGAAGGAACCTTACTTCTGTGGTGTGACATAATTGGACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATATAAAATTTTTAAGTGTATAATGTGTTAAACTACTGGTTCTACGCTACATACTCATATACCGAGTTCGGACACGTCCTTTAGCACAGTCCGCTAGAGAAACACTTCCTTGGAATGAAGACACCACACTGTATTAACCTGTTTGATGGATGTCTCTAAATTTCGAGATTCCATTTATATTTTAAAAATTCACATATTACACAATTTGATGACBarIQD256AM258YE260YM262AQ264AC266AG268NR270VR272RS274LC276EG278TL280LL282WC284DI286IG288QT290TY292RD294LK296L*2985889-6782source:PGKdtabpAORF frame 1SV40_3_spliceSV40_intSalIEcoRI fragment6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ATTCTAATTGTTTGTGTATTTTAGATTCCAACCTATGGAACTGATGAATGGGAGCAGTGGTGGAATGCAGATCCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCATAAGATTAACAAACACATAAAATCTAAGGTTGGATACCTTGACTACTTACCCTCGTCACCACCTTACGTCTAGGATCTCGAGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTBclIsource:PGKdtabpAbGH_PA_terminatorSV40_3_spliceBGH_re...rimerSalIEcoRI fragment6,8406,8606,8806,9006,9206,9406,9606,9807,000
    CTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGACCCCsource:PGKdtabpAbGH_PA_terminatorSalIEcoRI fragment7,0207,0407,0607,0807,1007,1207,1407,160
    CTCGAGATCCACTAGTTCTAGCCTCGAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGAGCTCTAGGTGATCAAGATCGGAGCTCCGTACGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAASpeITliITliIXhoIXhoIPspXIPaeR7IPaeR7Isource:PGKdtabpArev prim...120/130SalIEcoRI fragment7,1807,2007,2207,2407,2607,2807,3007,3207,340
    GCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGCGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCSapIBspQISalIEcoRI fragment7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTPciIAflIIIRep (pMB1)SalIEcoRI fragment7,5407,5607,5807,6007,6207,6407,6607,6807,700
    CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGARep (pMB1)SalIEcoRI fragment7,7207,7407,7607,7807,8007,8207,8407,860
    GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTCCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGARep (pMB1)SalIEcoRI fragment7,8807,9007,9207,9407,9607,9808,0008,0208,040
    AACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCTTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGRep (pMB1)SalIEcoRI fragment8,0608,0808,1008,1208,1408,1608,1808,2008,220
    CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAAAmpSalIEcoRI fragment8,2408,2608,2808,3008,3208,3408,3608,3808,400
    AATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTAhdIAmpSalIEcoRI fragment8,4208,4408,4608,4808,5008,5208,5408,560
    GCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCACGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTFspIAmpSalIEcoRI fragment8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAmpSalIEcoRI fragment8,7608,7808,8008,8208,8408,8608,8808,9008,920
    TTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACBcgIScaIXmnIAmpSalIEcoRI fragment8,9408,9608,9809,0009,0209,0409,0609,0809,100
    TTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTSspIAmpSalIEcoRI fragment9,1209,1409,1609,1809,2009,2209,2409,260
    GCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGSalIEcoRI fragment9,2809,3009,3209,3409,3609,3809,4009,4209,440
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9449 bp70007500800085009000500100015002000bamhI ecoRI fragmentsource:PGKdtabpASV40\EEL\poly(A)sMCSWPREGFPAmp114_HR130_GFP_seqRV5_FOR_HR130DTA_REVFLAGV5_FOR_HR130DTA_REVV5_FOR_HR130DTA_FWDV5V5p1_pcr_LSalIEcoRI fragment515-568407-448482-505455-481NdeI,BstAPI,FspIEcoRI fusion site,V5_FOR_HR130DTA_FWD,EcoRI fusion site,HA,2A\Peptide,+1BspQI,SapI,PmlI,XmnIPmlI,FseIPaqCIPspOMI,ApaIXcmI,SalIAccIAcc65IKpnIAflII3'dLTRPaeR7ITliIrev primer to clone into HR120/130,XhoI,SpeI,+4BspQI,SapIAflIIIPciIRep (pMB1)AhdIFspIScaI,BcgIXmnI,SspI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    9449