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pHS_SaCas9
  • Sequence Map
    Editing disabled on read-only sequence.
    ctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacAclIFspIBsrDIAmpR20406080100120140160
    ttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcaPvuIAmpR180200220240260280300320340
    gtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatccatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagAclIBssSIBssSαIAmpR360380400420440460480500520
    ttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaaaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttSspIBciVIBspHI540560580600620640660680700
    tgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttcacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagF1 ori720740760780800820840860
    tcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaaDrdINaeINgoMIVF1 ori8809009209409609801,0001,0201,040
    ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttccattcgcgaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaaggtaagcgAloIApoIApoIPsiISspIF1 ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaATTgtaatacgactcactataggggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactTAAcattatgctgagtgatatcccFspIPvuILacZ alphaT7M13-fwd1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cgaattGGAGCTCCACCGCGGTGGCGGCCCTAGAGACGGCGGggagctcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatagcttaaCCTCGAGGTGGCGCCACCGCCGGGATCTCTGCCGCCcctcgagcgactccgaactgtactaaccacgcatacaaacatacttcgatgtcctgactaaaccgcccgatactcccgcccccttcgagaccttcccggcgctaccccgcgcgccgcaggtcttccgcggtatKasINarISacISacISfoIBbvCIBsmBIEsp3IPluTIBssHIIEco53kIEco53kIHsp70A1,4201,4401,4601,4801,5001,5201,5401,560
    cggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttatggcccccaagaagaagcgcaaggtgggccgggcgaccgccgtgggtaggccatattttcgggcgctggggcttgccactggaggtgaaagtcgctgtttgctcgtgaatatgtatgcgctgataagacggcgatatgtattggtgagtcgatcgaattctagggtagttcgaataccgggggttcttcttcgcgttccaccBmtINheIAflIIBstEIIHindIIIMA2PK4KK6RK8V1723-5046Hsp70A5´UTR HSP70ASaCas91,5801,6001,6201,6401,6601,6801,7001,7201,740
    gcatccacggcgtgcccgccgccaagcgcaactacatcctgggcctggacatcggcatcaccagcgtgggctacggcatcatcgactacgagacccgcgacgtgatcgacgcgggcgtgcgcctgttcaaggaggccaacgtggagaacaacgagggccgccgcagcaagcgcggcgtaggtgccgcacgggcggcggttcgcgttgatgtaggacccggacctgtagccgtagtggtcgcacccgatgccgtagtagctgatgctctgggcgctgcactagctgcgcccgcacgcggacaagttcctccggttgcacctcttgttgctcccggcggcgtcgttcgcgccKasIG10IH12GV14PA16AK18RN20YI22LG24LD26IG28IT30SV32GY34GI36ID38YE40TR42DV44ID46AG48VR50LF52KE54AN56VE58NN60EG62RR64SK66RG681723-5046SaCas91,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgcccgccgcctgaagcgccgccgccgccaccgcatccagcgcgtgaagaagctgctgttcgactacaacctgctgaccgaccacagcgagctgagcggcatcaacccctacgaggcccgcgtgaagggcctgagccagaagctgagcgaggaggagttcagcgccgccctgctggcgggcggcggacttcgcggcggcggcggtggcgtaggtcgcgcacttcttcgacgacaagctgatgttggacgactggctggtgtcgctcgactcgccgtagttggggatgctccgggcgcacttcccggactcggtcttcgactcgctcctcctcaagtcgcggcgggacgacNarISfoIPluTIAR70RL72KR74RR76RH78RI80QR82VK84KL86LF88DY90NL92LT94DH96SE98LS100GI102NP104YE106AR108VK110GL112SQ114KL116SE118EE120FS122AA124LL1261723-5046SaCas91,9401,9601,9802,0002,0202,0402,0602,0802,100
    cacctggccaagcgccgcggcgtgcacaacgtgaacgaggtggaggaggacaccggcaacgagctgtccaccaaggagcagatcagccgcaacagcaaggccctggaggagaagtacgtggccgagctgcagctggagcgcctgaagaaggacggcgaggtgcgcggcagcatcagtggaccggttcgcggcgccgcacgtgttgcacttgctccacctcctcctgtggccgttgctcgacaggtggttcctcgtctagtcggcgttgtcgttccgggacctcctcttcatgcaccggctcgacgtcgacctcgcggacttcttcctgccgctccacgcgccgtcgtagtMscIPstIHL128AK130RR132GV134HN136VN138EV140EE142DT144GN146EL148ST150KE152QI154SR156NS158KA160LE162EK164YV166AE168LQ170LE172RL174KK176DG178EV180RG182SI1841723-5046SaCas92,1202,1402,1602,1802,2002,2202,2402,260
    accgcttcaagaccagcgactacgtgaaggaggcgaagcagctgctgaaggtgcagaaggcctaccaccagctggaccagagcttcatcgacacctacatcgacctgctggagacccgccgcacctactacgagggccccggcgagggcagccccttcggctggaaggacatcaatggcgaagttctggtcgctgatgcacttcctccgcttcgtcgacgacttccacgtcttccggatggtggtcgacctggtctcgaagtagctgtggatgtagctggacgacctctgggcggcgtggatgatgctcccggggccgctcccgtcggggaagccgaccttcctgtagttApaIStuIPflMIPspOMINR186FK188TS190DY192VK194EA196KQ198LL200KV202QK204AY206HQ208LD210QS212FI214DT216YI218DL220LE222TR224RT226YY228EG230PG232EG234SP236FG238WK240DI242K1723-5046SaCas92,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggagtggtacgagatgctgatgggccactgcacctacttccccgaggagctgcgctccgtgaagtacgcctacaacgccgacctgtacaacgccctgaacgacctgaacaacctggtcatcacccgcgacgagaacgagaagctggagtactacgagaagttccagatcatcgagcctcaccatgctctacgactacccggtgacgtggatgaaggggctcctcgacgcgaggcacttcatgcggatgttgcggctggacatgttgcgggacttgctggacttgttggaccagtagtgggcgctgctcttgctcttcgacctcatgatgctcttcaaggtctagtagctcSexAIE244WY246EM248LM250GH252CT254YF256PE258EL260RS262VK264YA266YN268AD270LY272NA274LN276DL278NN280LV282IT284RD286EN288EK290LE292YY294EK296FQ298II300E1723-5046SaCas92,4602,4802,5002,5202,5402,5602,5802,6002,620
    aacgtgttcaagcagaagaagaagcccaccctgaagcagatcgccaaggagatcctggtcaacgaggaggacatcaagggctaccgcgtgaccagcaccggcaagcccgagttcaccaacctgaaggtgtaccacgacatcaaggacatcaccgcccgcaaggagatcatcgagattgcacaagttcgtcttcttcttcgggtgggacttcgtctagcggttcctctaggaccagttgctcctcctgtagttcccgatggcgcactggtcgtggccgttcgggctcaagtggttggacttccacatggtgctgtagttcctgtagtggcgggcgttcctctagtagctctHincIIN302VF304KQ306KK308KP310TL312KQ314IA316KE318IL320VN322EE324DI326KG328YR330VT332ST334GK336PE338FT340NL342KV344YH346DI348KD350IT352AR354KE356II358E1723-5046SaCas92,6402,6602,6802,7002,7202,7402,7602,7802,800
    acgccgagctgctggaccagatcgcgaagatcctgaccatctaccagagcagcgaggacatccaggaggagctgaccaacctgaactccgagctgacccaggaggagatcgagcagatctcgaacctgaagggctacaccggcacccacaacctgagcctgaaggccatcaaccttgcggctcgacgacctggtctagcgcttctaggactggtagatggtctcgtcgctcctgtaggtcctcctcgactggttggacttgaggctcgactgggtcctcctctagctcgtctagagcttggacttcccgatgtggccgtgggtgttggactcggacttccggtagttggaNruIPfoIBglIIN360AE362LL364DQ366IA368KI370LT372IY374QS376SE378DI380QE382EL384TN386LN388SE390LT392QE394EI396EQ398IS400NL402KG404YT406GT408HN410LS412LK414AI416NL4181723-5046SaCas92,8202,8402,8602,8802,9002,9202,9402,960
    gatcctggacgagctgtggcacaccaacgacaaccagatcgccatcttcaaccgcctgaagctggtgcccaagaaggtggacctgtcccagcagaaggagatccccaccaccctggtggacgacttcatcctgagccccgtcgtgaagcgcagcttcatccagagcatcaaggtgctaggacctgctcgacaccgtgtggttgctgttggtctagcggtagaagttggcggacttcgaccacgggttcttccacctggacagggtcgtcttcctctaggggtggtgggaccacctgctgaagtaggactcggggcagcacttcgcgtcgaagtaggtctcgtagttccacPfoIBsmFIIL420DE422LW424HT426ND428NQ430IA432IF434NR436LK438LV440PK442KV444DL446SQ448QK450EI452PT454TL456VD458DF460IL462SP464VV466KR468SF470IQ472SI474KV4761723-5046SaCas92,9803,0003,0203,0403,0603,0803,1003,1203,140
    atcaacgccatcatcaagaagtacggcctgcccaacgacatcatcatcgagctggcccgcgagaagaactccaaggacgcccagaagatgatcaacgagatgcagaagcgcaaccgccagaccaacgagcgcatcgaggagatcatccgcaccaccggcaaggagaacgccaagttagttgcggtagtagttcttcatgccggacgggttgctgtagtagtagctcgaccgggcgctcttcttgaggttcctgcgggtcttctactagttgctctacgtcttcgcgttggcggtctggttgctcgcgtagctcctctagtaggcgtggtggccgttcctcttgcggttcaIN478AI480IK482KY484GL486PN488DI490II492EL494AR496EK498NS500KD502AQ504KM506IN508EM510QK512RN514RQ516TN518ER520IE522EI524IR526TT528GK530EN532AK5341723-5046SaCas93,1603,1803,2003,2203,2403,2603,2803,3003,320
    acctgatcgagaagatcaagctgcacgacatgcaggagggcaagtgcctgtactccctggaggccatcccgctggaggacctgctgaacaaccccttcaactacgaggtggaccacatcatcccccgcagcgtgtccttcgacaacagcttcaacaacaaggtgctggtcaagcatggactagctcttctagttcgacgtgctgtacgtcctcccgttcacggacatgagggacctccggtagggcgacctcctggacgacttgttggggaagttgatgctccacctggtgtagtagggggcgtcgcacaggaagctgttgtcgaagttgttgttccacgaccagttcgtPpuMIBstAPIYL536IE538KI540KL542HD544MQ546EG548KC550LY552SL554EA556IP558LE560DL562LN564NP566FN568YE570VD572HI574IP576RS578VS580FD582NS584FN586NK588VL590VK592Q1723-5046SaCas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggaggagaacagcaagaagggcaaccgcaccccgttccagtacctgagcagcagcgacagcaagatcagctacgagaccttcaagaagcacatcctgaacctggcgaagggcaagggccgcatctccaagaccaagaaggagtacctgctggaggagcgcgacatcaaccgcttccctcctcttgtcgttcttcccgttggcgtggggcaaggtcatggactcgtcgtcgctgtcgttctagtcgatgctctggaagttcttcgtgtaggacttggaccgcttcccgttcccggcgtagaggttctggttcttcctcatggacgacctcctcgcgctgtagttggcgaagE594EN596SK598KG600NR602TP604FQ606YL608SS610SD612SK614IS616YE618TF620KK622HI624LN626LA628KG630KG632RI634SK636TK638KE640YL642LE644ER646DI648NR650F1723-5046SaCas93,5203,5403,5603,5803,6003,6203,6403,660
    tccgtgcagaaggacttcatcaaccgcaacctggtggacacccgctacgccacccgcggcctgatgaacctgctgcgctcgtacttccgcgtgaacaacctggacgtgaaggtgaagtccatcaacggcggcttcacctcgttcctgcgccgcaagtggaagttcaagaaggagcaggcacgtcttcctgaagtagttggcgttggaccacctgtgggcgatgcggtgggcgccggactacttggacgacgcgagcatgaaggcgcacttgttggacctgcacttccacttcaggtagttgccgccgaagtggagcaaggacgcggcgttcaccttcaagttcttcctcgSexAIS652VQ654KD656FI658NR660NL662VD664TR666YA668TR670GL672MN674LL676RS678YF680RV682NN684LD686VK688VK690SI692NG694GF696TS698FL700RR702KW704KF706KK708E1723-5046SaCas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcaacaagggctacaagcaccacgccgaggacgccctgatcatcgccaacgccgacttcatcttcaaggagtggaagaagctggacaaggccaagaaggtgatggagaaccagatgttcgaggagaagcaggccgagagcatgcccgagatcgagaccgagcaggagtacaaggacgttgttcccgatgttcgtggtgcggctcctgcgggactagtagcggttgcggctgaagtagaagttcctcaccttcttcgacctgttccggttcttccactacctcttggtctacaagctcctcttcgtccggctctcgtacgggctctagctctggctcgtcctcatgttcctAjuISphIR710NK712GY714KH716HA718ED720AL722II724AN726AD728FI730FK732EW734KK736LD738KA740KK742VM744EN746QM748FE750EK752QA754ES756MP758EI760ET762EQ764EY766KE7681723-5046SaCas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    gatcttcatcaccccccaccagatcaagcacatcaaggacttcaaggactacaagtacagccaccgcgtggacaagaagccgaaccgcgagctgatcaacgacaccctgtacagcacccgcaaggacgacaagggcaacaccctgatcgtgaacaacctgaacggcctgtacgacctagaagtagtggggggtggtctagttcgtgtagttcctgaagttcctgatgttcatgtcggtggcgcacctgttcttcggcttggcgctcgactagttgctgtgggacatgtcgtgggcgttcctgctgttcccgttgtgggactagcacttgttggacttgccggacatgctgBglIIPflMIIF770IT772PH774QI776KH778IK780DF782KD784YK786YS788HR790VD792KK794PN796RE798LI800ND802TL804YS806TR808KD810DK812GN814TL816IV818NN820LN822GL824YD8261723-5046SaCas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    aaggacaacgacaagctgaagaagctgatcaacaagagccccgagaagctgctgatgtaccaccacgacccccagacctaccagaagctgaagctgatcatggagcagtacggcgacgagaagaaccccctgtacaagtactacgaggagaccggcaactacctgaccaagtactttcctgttgctgttcgacttcttcgactagttgttctcggggctcttcgacgactacatggtggtgctgggggtctggatggtcttcgacttcgactagtacctcgtcatgccgctgctcttcttgggggacatgttcatgatgctcctctggccgttgatggactggttcatgaKD828ND830KL832KK834LI836NK838SP840EK842LL844MY846HH848DP850QT852YQ854KL856KL858IM860EQ862YG864DE866KN868PL870YK872YY874EE876TG878NY880LT882KY8841723-5046SaCas94,2204,2404,2604,2804,3004,3204,3404,360
    ccaagaaggacaacggccccgtgatcaagaagatcaagtactacggcaacaagctgaacgcccacctggacatcaccgacgactaccccaacagccgcaacaaggtcgtgaagctgtccctgaagccctaccgcttcgacgtgtacctggacaacggcgtgtacaagttcgtgacggttcttcctgttgccggggcactagttcttctagttcatgatgccgttgttcgacttgcgggtggacctgtagtggctgctgatggggttgtcggcgttgttccagcacttcgacagggacttcgggatggcgaagctgcacatggacctgttgccgcacatgttcaagcactgBsmFISK886KD888NG890PV892IK894KI896KY898YG900NK902LN904AH906LD908IT910DD912YP914NS916RN918KV920VK922LS924LK926PY928RF930DV932YL934DN936GV938YK940FV942T1723-5046SaCas94,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgtgaagaacctggacgtgatcaagaaggagaactactacgaggtgaacagcaagtgctacgaggaggccaagaagctgaagaagatcagcaaccaggccgagttcatcgcctcgttctacaacaacgacctgatcaagatcaacggcgagctgtaccgcgtgatcggcgtgaacgcacttcttggacctgcactagttcttcctcttgatgatgctccacttgtcgttcacgatgctcctccggttcttcgacttcttctagtcgttggtccggctcaagtagcggagcaagatgttgttgctggactagttctagttgccgctcgacatggcgcactagccgcacttgV944KN946LD948VI950KK952EN954YY956EV958NS960KC962YE964EA966KK968LK970KI972SN974QA976EF978IA980SF982YN984ND986LI988KI990NG992EL994YR996VI998GV1000N1723-5046SaCas94,5604,5804,6004,6204,6404,6604,6804,7004,720
    aacgacctgctgaaccgcatcgaggtgaacatgatcgacatcacctaccgcgagtacctggagaacatgaacgacaagcgccccccccgcatcatcaagaccatcgccagcaagacccagtcgatcaagaagtacagcaccgacatcctgggcaacctgtacgaggtgaagtcgattgctggacgacttggcgtagctccacttgtactagctgtagtggatggcgctcatggacctcttgtacttgctgttcgcggggggggcgtagtagttctggtagcggtcgttctgggtcagctagttcttcatgtcgtggctgtaggacccgttggacatgctccacttcagctPflFITth111IN1002DL1004LN1006RI1008EV1010NM1012ID1014IT1016YR1018EY1020LE1022NM1024ND1026KR1028PP1030RI1032IK1034TI1036AS1038KT1040QS1042IK1044KY1046ST1048DI1050LG1052NL1054YE1056VK1058S1723-5046SaCas94,7404,7604,7804,8004,8204,8404,8604,8804,900
    agaagcacccccagatcatcaagaagggcagcggcaagcgccccgcggccaccaagaaggccggccaggccaagaagaagaagggcagctacccctacgacgtgcccgactacgcctacccgtacgacgtgccggactacgcctaaggatccccgctccgtgtaaatggaggcgctcttcgtgggggtctagtagttcttcccgtcgccgttcgcggggcgccggtggttcttccggccggtccggttcttcttcttcccgtcgatggggatgctgcacgggctgatgcggatgggcatgctgcacggcctgatgcggattcctaggggcgaggcacatttacctccgcgFseINaeISfiIBamHIBsiWIBsu36INgoMIVK1060KH1062PQ1064II1066KK1068GS1070GK1072RP1074AA1076TK1078KA1080GQ1082AK1084KK1086KG1088SY1090PY1092DV1094PD1096YA1098YP1100YD1102VP1104DY1106A*11081723-5046SaCas9NLSRbcs2\Term.4,9204,9404,9604,9805,0005,0205,0405,060
    tcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggccagcaactagactcggaacgggggactgcttgccgccacctaccttctatgacgagagttcacgacttcgccatcgaatcgaggggcaaagcacgactagtcagaaaaagttgtgcatttttcgcctcctcaaaacgttaaaacaaccaacattgctaggaggcaactaaaaccggRbcs2\Term.5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tctttctccatgggcgggctgggcgtatttgaagcgggtaccagcttttgttccctttagtgagggttaatttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctagaaagaggtacccgcccgacccgcataaacttcgcccatggtcgaaaacaagggaaatcactcccaattaaagctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaKpnINcoIAcc65IRbcs2\Term.lac promoterLacOM13-revT3T3 promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccaSapITfiIBspQI5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatgcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtaPciITfiIColE1 origin5,6205,6405,6605,6805,7005,7205,7405,760
    cacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgaDrdIBciVIBssSIBssSαIColE1 origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgagcatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctcColE1 origin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcColE1 origin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttBspHIColE1 origin6,3206,3406,3606,3806,4006,4206,4406,460
    gtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaccccatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggAhdIBsrDIAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    acgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgctgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcgAmpR6,6606,6806,7006,720
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6734 bp50005500600050010001500Hsp70A5´UTR HSP70AT3 promoterpHS XhoI R (DNA)pHS BamHI F (DNA)

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    6734