Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pBT44c intC.dCas9.UGI
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttgctgaggagacttAgggactcTcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtatgatatattcagggagaccacgtttattttgctttccgagtcagctttctgacccggaaagcaaaacgactcctctgaaTccctgagAgtgtcgattgtggtgcagcagggatagacgacgggatccagatactcaccaacgacctattgaaatgcccgtacgtattccgagcatactatataagtccctctggtgBcgIBmrIBsaISphIAvrIIBbvCIBmgBIinsulated proCrrnB1 rho-independe...ptional terminatorstem loopT-rich20406080100120140160
    aacggtttccctctacaaataattttgtttaacttttactagagtgggaccctacctgcaggtgcagtaaggaggaaaaaaaaatgatcaagatagctacaaggaagtatcttggcaaacaaaacgtttatgatattggagtcgaaagagatcacaactttgctctgaagaacggttgccaaagggagatgtttattaaaacaaattgaaaatgatctcaccctgggatggacgtccacgtcattcctcctttttttttactagttctatcgatgttccttcatagaaccgtttgttttgcaaatactataacctcagctttctctagtgttgaaacgagacttcttgccAclIBsgIKflIPstISbfISfcIPaqCIPpuMIEcoO109IM1I2K3I4A5T6R7K8Y9L10G11K12Q13N14V15Y16D17I18G19V20E21R22D23H24N25F26A27L28K29N30G31259-363Npu DnaE-Cinsulated proCSD8 RBS180200220240260280300320340
    attcatagcttctAATtGTTTtaaCaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttattttaagtatcgaagaTTAaCAAAattGtttatgagttatccgaatcgatagccgtgtttatcgcagcctacccgccactagtgactacttatattccaaggcagatttttcaagttccaagaccctttatgtctggcggtgtcatagttttttttagaatatccccgagaaaataaaBlpIFN2KY4SI6GL8AI10GT12NS14VG16WA18VI20TD22EY24KV26PS28KK30FK32VL34GN36TD38RH40SI42KK44NL46IG48AL50LF52370-4470F32I33A34S35259-363N1C2dCas9Npu...-C360380400420440460480500520
    gacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagactgtcacctctctgtcgccttcgctgagcagagtttgcctgtcgagcatcttccatatgtgcagccttcttagcataaacaatagatgtcctctaaaaaagtttactctaccgctttcatctactatcaaagaaagtagctgaacttctcagaaaaaaccaccttcttctgttctSfcIBmgBIBsmBIEsp3IBstZ17IDS54GE56TA58EA60TR62LK64RT66AR68RR70YT72RR74KN76RI78CY80LQ82EI84FS86NE88MA90KV92DD94SF96FH98RL100EE102SF104LV106EE108DK110370-4470dCas9540560580600620640660680700
    agcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattcgtacttgcagtaggataaaaacctttatatcatctacttcaacgaatagtactctttataggttgatagatagtagacgctttttttaaccatctaagatgactatttcgcctaaacgcgaattagataaaccggaatcgcgtatactaattcaaagcaccagtaaaaaactaNdeIKH112ER114HP116IF118GN120IV122DE124VA126YH128EK130YP132TI134YH136LR138KK140LV142DS144TD146KA148DL150RL152IY154LA156LA158HM160IK162FR164GH166FL168I370-4470dCas9720740760780800820840860
    tgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagactccctctaaatttaggactattatcactacacctgtttgataaataggtcaaccatgtttggatgttagttaataaacttcttttgggataattgcgttcacctcatctacgatttcgctaagaaagacgtgctaactcatttagttctgctaatcttttagagtaacgagtcBlpIBsgISwaIBsrDIE170GD172LN174PD176NS178DV180DK182LF184IQ186LV188QT190YN192QL194FE196EN198PI200NA202SG204VD206AK208AI210LS212AR214LS216KS218RR220LE222NL224IA226Q370-4470dCas98809009209409609801,0001,0201,040
    ctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatggaggggccactcttctttttaccgaataaacccttagagtaacgaaacagtaacccaaactggggattaaaatttagtttaaaactaaaccgtcttctacgatttaatgtcgaaagttttctatgaatgctactactaaatctattaaataaccgcgtttaacctctagttatacBsrDIBstAPIL228PG230EK232KN234GL236FG238NL240IA242LS244LG246LT248PN250FK252SN254FD256LA258ED260AK262LQ264LS266KD268TY270DD272DL274DN276LL278AQ280IG282DQ284Y370-4470dCas91,0601,0801,1001,1201,1401,1601,1801,2001,220
    ctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagagactaaacaaaaaccgtcgattcttaaatagtctacgataaaatgaaagtctataggattctcatttatgactttattgattccgaggggatagtcgaagttactaatttgcgatgctacttgtagtagttctgaactgagaaaattttcgaaatcaagctgttgttgaaggtctBsaXIHindIIIA286DL288FL290AA292KN294LS296DA298IL300LS302DI304LR306VN308TE310IT312KA314PL316SA318SM320IK322RY324DE326HH328QD330LT332LL334KA336LV338RQ340QL342PE344370-4470dCas91,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggtttcatatttctttagaaaaaactagttagttttttgcctatacgtccaatataactaccccctcgatcggttcttcttaaaatatttaaatagtttggttaaaatcttttttacctaccatgactccttaataaccactttgatttagcacttctaaacgacgcgttcgttgccBmtIFspINheIKY346KE348IF350FD352QS354KN356GY358AG360YI362DG364GA366SQ368EE370FY372KF374IK376PI378LE380KM382DG384TE386EL388LV390KL392NR394ED396LL398RK400QR402370-4470dCas91,4201,4401,4601,4801,5001,5201,5401,560
    acctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgaattccttattatgttggtccattggcgcgtggcaatagtcgtttggaaactgttgccgagataaggggtagtttaagtgaacccactcgacgtacgataaaactcttctgttcttctgaaaataggtaaaaattttctgttagcactcttctaacttttttagaactgaaaagcttaaggaataatacaaccaggtaaccgcgcaccgttatcagcaaAleISphIBstBIEcoRITF404DN406GS408IP410HQ412IH414LG416EL418HA420IL422RR424QE426DF428YP430FL432KD434NR436EK438IE440KI442LT444FR446IP448YY450VG452PL454AR456GN458SR460370-4470dCas91,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtaacgtacctactgagccttcagacttctttgttaatggggtaccttaaaacttcttcaacagctatttccacgaagtcgagttagtaaataacttgcgtactgtttgaaactatttttagaaggtttactttttcatgatggttttgtatcaaacgaaatactcataaaatgccaBcgINcoIScaITatIFA462WM464TR466KS468EE470TI472TP474WN476FE478EV480VD482KG484AS486AQ488SF490IE492RM494TN496FD498KN500LP502NE504KV506LP508KH510SL512LY514EY516FT518V370-4470dCas91,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttaatattgcttaactgtttccagtttatacaatgacttccttacgcttttggtcgtaaagaaagtccacttgtcttctttcggtaacaactaaatgagaagttttgtttagcttttcattggcaattcgttaattttcttctaataaagtttttttatcttacaaaactatcacaaPshAIY520NE522LT524KV526KY528VT530EG532MR534KP536AF538LS540GE542QK544KA546IV548DL550LF552KT554NR556KV558TV560KQ562LK564ED566YF568KK570IE572CF574DS576V370-4470dCas91,9401,9601,9802,0002,0202,0402,0602,0802,100
    gaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttactttaaagtcctcaacttctatctaaattacgaagtaatccatggatggtactaaacgatttttaataatttctatttctaaaaaacctattacttcttttacttctatagaatctcctataacaaaattgtaactggaataaacttctatccctctactaactcctttctgaatBanIKpnIAcc65IE578IS580GV582ED584RF586NA588SL590GT592YH594DL596LK598II600KD602KD604FL606DN608EE610NE612DI614LE616DI618VL620TL622TL624FE626DR628EM630IE632ER634L370-4470dCas92,1202,1402,1602,1802,2002,2202,2402,260
    aaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcatttgtatacgagtggagaaactactattccactactttgtcgaatttgcagcggcaatatgaccaacccctgcaaacagagcttttaactaattaccataatccctattcgttagaccgttttgttataatctaaaaaactttagtctaccaaaacggttagcgttaaaatacgtNdeIPshAIK636TY638AH640LF642DD644KV646MK648QL650KR652RR654YT656GW658GR660LS662RK664LI666NG668IR670DK672QS674GK676TI678LD680FL682KS684DG686FA688NR690NF692MQ694370-4470dCas92,2802,3002,3202,3402,3602,3802,4002,4202,440
    gctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcgactaggtactactatcaaactgtaaatttcttctgtaagtttttcgtgttcacagacctgttccgctatcaaatgtacttgtataacgtttaaatcgaccatcgggacgataattttttccataaaatgtctgacattttcaacaactacttaaccagtttcattaccccgccPvuIILI696HD698DS700LT702FK704ED706IQ708KA710QV712SG714QG716DS718LH720EH722IA724NL726AG728SP730AI732KK734GI736LQ738TV740KV742VD744EL746VK748VM750GR752370-4470dCas92,4602,4802,5002,5202,5402,5602,5802,6002,620
    cataagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctgtattcggtcttttatagcaataactttaccgtgcacttttagtctgttgagttttcccggtctttttaagcgctctcgcatactttgcttagcttcttccatagtttcttaatccttcagtctaagaatttctcgtaggacaacttttatgagttaacgttttacttttcgagaNruIPmlIHK754PE756NI758VI760EM762AR764EN766QT768TQ770KG772QK774NS776RE778RM780KR782IE784EG786IK788EL790GS792QI794LK796EH798PV800EN802TQ804LQ806NE808KL810370-4470dCas92,6402,6602,6802,7002,7202,7402,7602,7802,800
    atctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttagagataatagaggttttaccttctctgtacatacacctggttcttaatctataattagcaaattcactaatactacagctacggtaacaaggtgtttcaaaggaatttctgctaagttatctgttattccagaattgcgcaagactatttttagcaccatttagcctattgcaAclIMluIPciIYL812YY814LQ816NG818RD820MY822VD824QE826LD828IN830RL832SD834YD836VD838AI840VP842QS844FL846KD848DS850ID852NK854VL856TR858SD860KN862RG864KS866DN868V370-4470dCas92,8202,8402,8602,8802,9002,9202,9402,960
    tccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaggttcacttcttcatcagtttttctactttttgataacctctgttgaagatttgcggttcaattagtgagttgcattcaaactattaaattgctttcgacttgcacctccaaactcacttgaactatttcgaccaaaatagtttgcggttaaccaactttgagcggtttagtgaP870SE872EV874VK876KM878KN880YW882RQ884LL886NA888KL890IT892QR894KF896DN898LT900KA902ER904GG906LS908EL910DK912AG914FI916KR918QL920VE922TR924QI926T370-4470dCas92,9803,0003,0203,0403,0603,0803,1003,1203,140
    aagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgttcgtacaccgtgtttaaaacctatcagcgtacttatgatttatgctacttttactatttgaataagctctccaatttcactaatggaattttagatttaatcaaagactgaaggcttttctaaaggttaagatatttcatgcactctaattgttaatggtagtacgggtactacK928HV930AQ932IL934DS936RM938NT940KY942DE944ND946KL948IR950EV952KV954IT956LK958SK960LV962SD964FR966KD968FQ970FY972KV974RE976IN978NY980HH982AH984D370-4470dCas93,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttgcatagatttacggcagcaaccttgacgaaactaattctttataggttttgaacttagcctcaaacagataccactaatatttcaaatactacaagcattttactaacgattcagactcgttctttatccgtttcgttggcgttttataaagaaaatgagattatagtacttgaaA986YL988NA990VV992GT994AL996IK998KY1000PK1002LE1004SE1006FV1008YG1010DY1012KV1014YD1016VR1018KM1020IA1022KS1024EQ1026EI1028GK1030AT1032AK1034YF1036FY1038SN1040IM1042NF1044370-4470dCas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    cttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcgaagttttgtctttaatgtgaacgtttacctctctaagcgtttgcgggagattagctttgattacccctttgacctctttaacagaccctatttcccgctctaaaacggtgtcacgcgtttcataacaggtacggggttcagttataacagttcttttgtcttcatgtctgtccgBpmIFspITatIBsaXIFK1046TE1048IT1050LA1052NG1054EI1056RK1058RP1060LI1062ET1064NG1066ET1068GE1070IV1072WD1074KG1076RD1078FA1080TV1082RK1084VL1086SM1088PQ1090VN1092IV1094KK1096TE1098VQ1100TG1102370-4470dCas93,5203,5403,5603,5803,6003,6203,6403,660
    ggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagcctaagaggttcctcagttaaaatggtttttctttaagcctgttcgaataacgagcattttttctgaccctaggtttttttataccaccaaaactatcaggttgccatcgaataagtcaggatcaccaacgattccacctttttccctttagcttcttcaattttaggcaatttcBmrIBamHIBstYIHindIIIGF1104SK1106ES1108IL1110PK1112RN1114SD1116KL1118IA1120RK1122KD1124WD1126PK1128KY1130GG1132FD1134SP1136TV1138AY1140SV1142LV1144VA1146KV1148EK1150GK1152SK1154KL1156KS1158VK1160370-4470dCas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    agttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaatttcaatgatccctagtgttaatacctttcttcaaggaaactttttttaggctaactgaaaaatcttcgatttcctatattccttcaattttttctgaattagtaatttgatggatttatatcagaaaaactcaatcttttgccagcatttgcctacgaccgatcacggcctcttaaEL1162LG1164IT1166IM1168ER1170SS1172FE1174KN1176PI1178DF1180LE1182AK1184GY1186KE1188VK1190KD1192LI1194IK1196LP1198KY1200SL1202FE1204LE1206NG1208RK1210RM1212LA1214SA1216GE1218L370-4470dCas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    acaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagtgtttttcctttactcgaccgagacggttcgtttatacacttaaaaaatataaatcgatcagtaatacttttcaacttcccatcaggtcttctattgcttgtttttgttaacaaacacctcgtcgtattcgtaataaatctactctaataactcgtttagtcacttaaaagattcQ1220KG1222NE1224LA1226LP1228SK1230YV1232NF1234LY1236LA1238SH1240YE1242KL1244KG1246SP1248ED1250NE1252QK1254QL1256FV1258EQ1260HK1262HY1264LD1266EI1268IE1270QI1272SE1274FS1276K370-4470dCas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgtgttattttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatagcacaataaaatcgtctacggttaaatctatttcaagaatcacgtatattgtttgtatctctgtttggttatgcacttgttcgtcttttataataagtaaataaatgcaactgcttagaacctcgagggcgacgaaaatttataaaactatgttgttaactagcatttgctatatFauISacIHincIIBsiHKAIEco53kIR1278VI1280LA1282DA1284NL1286DK1288VL1290SA1292YN1294KH1296RD1298KP1300IR1302EQ1304AE1306NI1308IH1310LF1312TL1314TN1316LG1318AP1320AA1322FK1324YF1326DT1328TI1330DR1332KR1334Y370-4470dCas94,2204,2404,2604,2804,3004,3204,3404,360
    cgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgacAGCGGCGGTTCAATGACAAATCTGTCGGATATCATCGAAAAAGAAACCGGTAAACAGCTGGTTATCCAAGAAAGTATTTTgcagatgttttcttcaaaatctacggtgagaataggtagttaggtagtgaccagaaatactttgtgcgtaactaaactcagtcgatcctccactgTCGCCGCCAAGTTACTGTTTAGACAGCCTATAGTAGCTTTTTCTTTGGCCATTTGTCGACCAATAGGTTCTTTCATAAAAAgeIPvuIIT1336ST1338KE1340VL1342DA1344TL1346IH1348QS1350IT1352GL1354YE1356TR1358ID1360LS1362QL1364GG1366D370-4470M1T2N3L4S5D6I7I8E9K10E11T12G13K14Q15L16V17I18Q19E20S21I22L234483-4737S1G2G3S44471-4482dCas9ugi4,3804,4004,4204,4404,4604,4804,5004,5204,540
    AATGCTTCCCGAAGAAGTCGAAGAGGTGATTGGCAACAAACCCGAAAGTGATATTCTCGTGCATACTGCGTATGATGAGTCGACCGATGAAAACGTCATGCTGCTGACCTCCGACGCGCCCGAGTATAAACCGTGGGCTTTGGTTATCCAGGATAGCAACGGTGAAAATAAGATTTTACGAAGGGCTTCTTCAGCTTCTCCACTAACCGTTGTTTGGGCTTTCACTATAAGAGCACGTATGACGCATACTACTCAGCTGGCTACTTTTGCAGTACGACGACTGGAGGCTGCGCGGGCTCATATTTGGCACCCGAAACCAATAGGTCCTATCGTTGCCACTTTTATTCTAAAvaIPfoISalIBsoBIBssSIBssSαIHincIIM24L25P26E27E28V29E30E31V32I33G34N35K36P37E38S39D40I41L42V43H44T45A46Y47D48E49S50T51D52E53N54V55M56L57L58T59S60D61A62P63E64Y65K66P67W68A69L70V71I72Q73D74S75N76G77E78N79K80I814483-4737ugi4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAAATGTTAtaaggcactcctcagcaaatataatgaccctcttgataacccaagagggcattttttaatgcccAtggcgtcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaTTTTACAATattccgtgaggagtcgtttatattactgggagaactattgggttctcccgtaaaaaattacgggTaccgcagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttNcoIBbvCIBsaHIK82M83L84*854483-4737aph (KanR)tetA/orfL bidirectional terminatorChen, Voigt e...th 0.84±0.56Chen, Voigt ...th 1.11±0.3ugi4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaataBstYIPflMIaph (KanR)4,9204,9404,9604,9805,0005,0205,0405,060
    gcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacgBtsIPvuIAsiSIBsmBIBtsαIEsp3Iaph (KanR)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagaAvaIBtsISmaIXmaIBsoBIBtsαIEcoNITspMIaph (KanR)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgcattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcClaINruIBspDIaph (KanR)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaAgagaaaccTacggatcggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactTctctttggAtgcctagccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgagPfoISacIIp15A Ori RNAIIp mutantRNA IIaph (KanR)US...25,6205,6405,6605,6805,7005,7205,7405,760
    cgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccagcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtAgeIEaeIBssSIBssSαIp15A Ori RNAIIp mutantRNA II5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatSmlIAlwNIBpuEIp15A Ori RNAIIp mutantRNA II5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagaTattacgcgcagaccaaaaccagaacttcagtacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctAtaatgcgcgtctggttttgBpuEIBstBIp15A Ori RNAIIp mutantRNA IIRNA IRNA I Promoter (0.96)RNA II Pro...er (0.92)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gatctcaagaagatcatcttattaaggagccccatacgatataagaggacatccggtctagagttcttctagtagaataattcctcggggtatgctatattctcctgtaggccaAhdISmlIp15A ...tantRNA ...92)6,3206,340
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    6357 bp450050005500600050010001500dCas9+1 TSSpRNAII G>T370-4470259-363364-369rrnB1 rho-independent transcriptional terminator,T-rich,stem loop,loop,+22from XTEN linker,BlpIBsmBIEsp3I,BstZ17I,BmgBI,SfcINdeI,SwaIBsgIBsrDI,BlpI,BsrDIBstAPIBsaXIHindIIINheI,BmtIFspItetA/orfL bidirectional terminator,+5PflMIBstYIaph (KanR),BsmBI,Esp3I,AsiSI,PvuIBtsI,BtsαI,EcoNI,AvaI,BsoBI,TspMI,XmaI,SmaIBtsI,BtsαIBspDIClaI,NruIUSER junction 22,prim transcript,PfoI,SacIIRNA II,p15A Ori RNAIIp mutant,BssSI,BssSαI,AgeI,EaeIRNA I Promoter (0.96),-35 -35 signal,-10 -10 signal,RNA I,RNA II Promoter (0.92),+9

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    6357