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pBT44c intC.dCas9.UGI
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttgctgaggagacttAgggactcTcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtatgatatattcagggagaccacgtttattttgctttccgagtcagctttctgacccggaaagcaaaacgactcctctgaaTccctgagAgtgtcgattgtggtgcagcagggatagacgacgggatccagatactcaccaacgacctattgaaatgcccgtacgtattccgagcatactatataagtccctctggtgBcgIBmrIBsaISphIAvrIIBbvCIBmgBIinsulated proCrrnB1 rho-independe...ptional terminatorstem loopT-rich20406080100120140160
    aacggtttccctctacaaataattttgtttaacttttactagagtgggaccctacctgcaggtgcagtaaggaggaaaaaaaaatgatcaagatagctacaaggaagtatcttggcaaacaaaacgtttatgatattggagtcgaaagagatcacaactttgctctgaagaacggttgccaaagggagatgtttattaaaacaaattgaaaatgatctcaccctgggatggacgtccacgtcattcctcctttttttttactagttctatcgatgttccttcatagaaccgtttgttttgcaaatactataacctcagctttctctagtgttgaaacgagacttcttgccAclIBsgIKflIPstISbfISfcIPaqCIPpuMIEcoO109IM1I2K3I4A5T6R7K8Y9L10G11K12Q13N14V15Y16D17I18G19V20E21R22D23H24N25F26A27L28K29N30G31259-363Npu DnaE-Cinsulated proCSD8 RBS180200220240260280300320340
    attcatagcttctAATtGTTTtaaCaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttattttaagtatcgaagaTTAaCAAAattGtttatgagttatccgaatcgatagccgtgtttatcgcagcctacccgccactagtgactacttatattccaaggcagatttttcaagttccaagaccctttatgtctggcggtgtcatagttttttttagaatatccccgagaaaataaaBlpIN1C2F32I33A34S35259-363FN2KY4SI6GL8AI10GT12NS14VG16WA18VI20TD22EY24KV26PS28KK30FK32VL34GN36TD38RH40SI42KK44NL46IG48AL50LF52370-4470dCas9Npu...-C360380400420440460480500520
    gacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagactgtcacctctctgtcgccttcgctgagcagagtttgcctgtcgagcatcttccatatgtgcagccttcttagcataaacaatagatgtcctctaaaaaagtttactctaccgctttcatctactatcaaagaaagtagctgaacttctcagaaaaaaccaccttcttctgttctSfcIBmgBIBsmBIEsp3IBstZ17IDS54GE56TA58EA60TR62LK64RT66AR68RR70YT72RR74KN76RI78CY80LQ82EI84FS86NE88MA90KV92DD94SF96FH98RL100EE102SF104LV106EE108DK110370-4470dCas9540560580600620640660680700
    agcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattcgtacttgcagtaggataaaaacctttatatcatctacttcaacgaatagtactctttataggttgatagatagtagacgctttttttaaccatctaagatgactatttcgcctaaacgcgaattagataaaccggaatcgcgtatactaattcaaagcaccagtaaaaaactaNdeIKH112ER114HP116IF118GN120IV122DE124VA126YH128EK130YP132TI134YH136LR138KK140LV142DS144TD146KA148DL150RL152IY154LA156LA158HM160IK162FR164GH166FL168I370-4470dCas9720740760780800820840860
    tgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagactccctctaaatttaggactattatcactacacctgtttgataaataggtcaaccatgtttggatgttagttaataaacttcttttgggataattgcgttcacctcatctacgatttcgctaagaaagacgtgctaactcatttagttctgctaatcttttagagtaacgagtcBlpIBsgISwaIBsrDIE170GD172LN174PD176NS178DV180DK182LF184IQ186LV188QT190YN192QL194FE196EN198PI200NA202SG204VD206AK208AI210LS212AR214LS216KS218RR220LE222NL224IA226Q370-4470dCas98809009209409609801,0001,0201,040
    ctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatggaggggccactcttctttttaccgaataaacccttagagtaacgaaacagtaacccaaactggggattaaaatttagtttaaaactaaaccgtcttctacgatttaatgtcgaaagttttctatgaatgctactactaaatctattaaataaccgcgtttaacctctagttatacBsrDIBstAPIL228PG230EK232KN234GL236FG238NL240IA242LS244LG246LT248PN250FK252SN254FD256LA258ED260AK262LQ264LS266KD268TY270DD272DL274DN276LL278AQ280IG282DQ284Y370-4470dCas91,0601,0801,1001,1201,1401,1601,1801,2001,220
    ctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagagactaaacaaaaaccgtcgattcttaaatagtctacgataaaatgaaagtctataggattctcatttatgactttattgattccgaggggatagtcgaagttactaatttgcgatgctacttgtagtagttctgaactgagaaaattttcgaaatcaagctgttgttgaaggtctBsaXIHindIIIA286DL288FL290AA292KN294LS296DA298IL300LS302DI304LR306VN308TE310IT312KA314PL316SA318SM320IK322RY324DE326HH328QD330LT332LL334KA336LV338RQ340QL342PE344370-4470dCas91,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggtttcatatttctttagaaaaaactagttagttttttgcctatacgtccaatataactaccccctcgatcggttcttcttaaaatatttaaatagtttggttaaaatcttttttacctaccatgactccttaataaccactttgatttagcacttctaaacgacgcgttcgttgccBmtIFspINheIKY346KE348IF350FD352QS354KN356GY358AG360YI362DG364GA366SQ368EE370FY372KF374IK376PI378LE380KM382DG384TE386EL388LV390KL392NR394ED396LL398RK400QR402370-4470dCas91,4201,4401,4601,4801,5001,5201,5401,560
    acctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgaattccttattatgttggtccattggcgcgtggcaatagtcgtttggaaactgttgccgagataaggggtagtttaagtgaacccactcgacgtacgataaaactcttctgttcttctgaaaataggtaaaaattttctgttagcactcttctaacttttttagaactgaaaagcttaaggaataatacaaccaggtaaccgcgcaccgttatcagcaaAleISphIBstBIEcoRITF404DN406GS408IP410HQ412IH414LG416EL418HA420IL422RR424QE426DF428YP430FL432KD434NR436EK438IE440KI442LT444FR446IP448YY450VG452PL454AR456GN458SR460370-4470dCas91,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtaacgtacctactgagccttcagacttctttgttaatggggtaccttaaaacttcttcaacagctatttccacgaagtcgagttagtaaataacttgcgtactgtttgaaactatttttagaaggtttactttttcatgatggttttgtatcaaacgaaatactcataaaatgccaBcgINcoIScaITatIFA462WM464TR466KS468EE470TI472TP474WN476FE478EV480VD482KG484AS486AQ488SF490IE492RM494TN496FD498KN500LP502NE504KV506LP508KH510SL512LY514EY516FT518V370-4470dCas91,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttaatattgcttaactgtttccagtttatacaatgacttccttacgcttttggtcgtaaagaaagtccacttgtcttctttcggtaacaactaaatgagaagttttgtttagcttttcattggcaattcgttaattttcttctaataaagtttttttatcttacaaaactatcacaaPshAIY520NE522LT524KV526KY528VT530EG532MR534KP536AF538LS540GE542QK544KA546IV548DL550LF552KT554NR556KV558TV560KQ562LK564ED566YF568KK570IE572CF574DS576V370-4470dCas91,9401,9601,9802,0002,0202,0402,0602,0802,100
    gaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttactttaaagtcctcaacttctatctaaattacgaagtaatccatggatggtactaaacgatttttaataatttctatttctaaaaaacctattacttcttttacttctatagaatctcctataacaaaattgtaactggaataaacttctatccctctactaactcctttctgaatBanIKpnIAcc65IE578IS580GV582ED584RF586NA588SL590GT592YH594DL596LK598II600KD602KD604FL606DN608EE610NE612DI614LE616DI618VL620TL622TL624FE626DR628EM630IE632ER634L370-4470dCas92,1202,1402,1602,1802,2002,2202,2402,260
    aaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcatttgtatacgagtggagaaactactattccactactttgtcgaatttgcagcggcaatatgaccaacccctgcaaacagagcttttaactaattaccataatccctattcgttagaccgttttgttataatctaaaaaactttagtctaccaaaacggttagcgttaaaatacgtNdeIPshAIK636TY638AH640LF642DD644KV646MK648QL650KR652RR654YT656GW658GR660LS662RK664LI666NG668IR670DK672QS674GK676TI678LD680FL682KS684DG686FA688NR690NF692MQ694370-4470dCas92,2802,3002,3202,3402,3602,3802,4002,4202,440
    gctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcgactaggtactactatcaaactgtaaatttcttctgtaagtttttcgtgttcacagacctgttccgctatcaaatgtacttgtataacgtttaaatcgaccatcgggacgataattttttccataaaatgtctgacattttcaacaactacttaaccagtttcattaccccgccPvuIILI696HD698DS700LT702FK704ED706IQ708KA710QV712SG714QG716DS718LH720EH722IA724NL726AG728SP730AI732KK734GI736LQ738TV740KV742VD744EL746VK748VM750GR752370-4470dCas92,4602,4802,5002,5202,5402,5602,5802,6002,620
    cataagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctgtattcggtcttttatagcaataactttaccgtgcacttttagtctgttgagttttcccggtctttttaagcgctctcgcatactttgcttagcttcttccatagtttcttaatccttcagtctaagaatttctcgtaggacaacttttatgagttaacgttttacttttcgagaNruIPmlIHK754PE756NI758VI760EM762AR764EN766QT768TQ770KG772QK774NS776RE778RM780KR782IE784EG786IK788EL790GS792QI794LK796EH798PV800EN802TQ804LQ806NE808KL810370-4470dCas92,6402,6602,6802,7002,7202,7402,7602,7802,800
    atctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttagagataatagaggttttaccttctctgtacatacacctggttcttaatctataattagcaaattcactaatactacagctacggtaacaaggtgtttcaaaggaatttctgctaagttatctgttattccagaattgcgcaagactatttttagcaccatttagcctattgcaAclIMluIPciIYL812YY814LQ816NG818RD820MY822VD824QE826LD828IN830RL832SD834YD836VD838AI840VP842QS844FL846KD848DS850ID852NK854VL856TR858SD860KN862RG864KS866DN868V370-4470dCas92,8202,8402,8602,8802,9002,9202,9402,960
    tccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaggttcacttcttcatcagtttttctactttttgataacctctgttgaagatttgcggttcaattagtgagttgcattcaaactattaaattgctttcgacttgcacctccaaactcacttgaactatttcgaccaaaatagtttgcggttaaccaactttgagcggtttagtgaP870SE872EV874VK876KM878KN880YW882RQ884LL886NA888KL890IT892QR894KF896DN898LT900KA902ER904GG906LS908EL910DK912AG914FI916KR918QL920VE922TR924QI926T370-4470dCas92,9803,0003,0203,0403,0603,0803,1003,1203,140
    aagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgttcgtacaccgtgtttaaaacctatcagcgtacttatgatttatgctacttttactatttgaataagctctccaatttcactaatggaattttagatttaatcaaagactgaaggcttttctaaaggttaagatatttcatgcactctaattgttaatggtagtacgggtactacK928HV930AQ932IL934DS936RM938NT940KY942DE944ND946KL948IR950EV952KV954IT956LK958SK960LV962SD964FR966KD968FQ970FY972KV974RE976IN978NY980HH982AH984D370-4470dCas93,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttgcatagatttacggcagcaaccttgacgaaactaattctttataggttttgaacttagcctcaaacagataccactaatatttcaaatactacaagcattttactaacgattcagactcgttctttatccgtttcgttggcgttttataaagaaaatgagattatagtacttgaaA986YL988NA990VV992GT994AL996IK998KY1000PK1002LE1004SE1006FV1008YG1010DY1012KV1014YD1016VR1018KM1020IA1022KS1024EQ1026EI1028GK1030AT1032AK1034YF1036FY1038SN1040IM1042NF1044370-4470dCas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    cttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcgaagttttgtctttaatgtgaacgtttacctctctaagcgtttgcgggagattagctttgattacccctttgacctctttaacagaccctatttcccgctctaaaacggtgtcacgcgtttcataacaggtacggggttcagttataacagttcttttgtcttcatgtctgtccgBpmIFspITatIBsaXIFK1046TE1048IT1050LA1052NG1054EI1056RK1058RP1060LI1062ET1064NG1066ET1068GE1070IV1072WD1074KG1076RD1078FA1080TV1082RK1084VL1086SM1088PQ1090VN1092IV1094KK1096TE1098VQ1100TG1102370-4470dCas93,5203,5403,5603,5803,6003,6203,6403,660
    ggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagcctaagaggttcctcagttaaaatggtttttctttaagcctgttcgaataacgagcattttttctgaccctaggtttttttataccaccaaaactatcaggttgccatcgaataagtcaggatcaccaacgattccacctttttccctttagcttcttcaattttaggcaatttcBmrIBamHIBstYIHindIIIGF1104SK1106ES1108IL1110PK1112RN1114SD1116KL1118IA1120RK1122KD1124WD1126PK1128KY1130GG1132FD1134SP1136TV1138AY1140SV1142LV1144VA1146KV1148EK1150GK1152SK1154KL1156KS1158VK1160370-4470dCas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    agttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaatttcaatgatccctagtgttaatacctttcttcaaggaaactttttttaggctaactgaaaaatcttcgatttcctatattccttcaattttttctgaattagtaatttgatggatttatatcagaaaaactcaatcttttgccagcatttgcctacgaccgatcacggcctcttaaEL1162LG1164IT1166IM1168ER1170SS1172FE1174KN1176PI1178DF1180LE1182AK1184GY1186KE1188VK1190KD1192LI1194IK1196LP1198KY1200SL1202FE1204LE1206NG1208RK1210RM1212LA1214SA1216GE1218L370-4470dCas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    acaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagtgtttttcctttactcgaccgagacggttcgtttatacacttaaaaaatataaatcgatcagtaatacttttcaacttcccatcaggtcttctattgcttgtttttgttaacaaacacctcgtcgtattcgtaataaatctactctaataactcgtttagtcacttaaaagattcQ1220KG1222NE1224LA1226LP1228SK1230YV1232NF1234LY1236LA1238SH1240YE1242KL1244KG1246SP1248ED1250NE1252QK1254QL1256FV1258EQ1260HK1262HY1264LD1266EI1268IE1270QI1272SE1274FS1276K370-4470dCas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgtgttattttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatagcacaataaaatcgtctacggttaaatctatttcaagaatcacgtatattgtttgtatctctgtttggttatgcacttgttcgtcttttataataagtaaataaatgcaactgcttagaacctcgagggcgacgaaaatttataaaactatgttgttaactagcatttgctatatFauISacIHincIIBsiHKAIEco53kIR1278VI1280LA1282DA1284NL1286DK1288VL1290SA1292YN1294KH1296RD1298KP1300IR1302EQ1304AE1306NI1308IH1310LF1312TL1314TN1316LG1318AP1320AA1322FK1324YF1326DT1328TI1330DR1332KR1334Y370-4470dCas94,2204,2404,2604,2804,3004,3204,3404,360
    cgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgacAGCGGCGGTTCAATGACAAATCTGTCGGATATCATCGAAAAAGAAACCGGTAAACAGCTGGTTATCCAAGAAAGTATTTTgcagatgttttcttcaaaatctacggtgagaataggtagttaggtagtgaccagaaatactttgtgcgtaactaaactcagtcgatcctccactgTCGCCGCCAAGTTACTGTTTAGACAGCCTATAGTAGCTTTTTCTTTGGCCATTTGTCGACCAATAGGTTCTTTCATAAAAAgeIPvuIIM1T2N3L4S5D6I7I8E9K10E11T12G13K14Q15L16V17I18Q19E20S21I22L234483-4737T1336ST1338KE1340VL1342DA1344TL1346IH1348QS1350IT1352GL1354YE1356TR1358ID1360LS1362QL1364GG1366D370-4470S1G2G3S44471-4482dCas9ugi4,3804,4004,4204,4404,4604,4804,5004,5204,540
    AATGCTTCCCGAAGAAGTCGAAGAGGTGATTGGCAACAAACCCGAAAGTGATATTCTCGTGCATACTGCGTATGATGAGTCGACCGATGAAAACGTCATGCTGCTGACCTCCGACGCGCCCGAGTATAAACCGTGGGCTTTGGTTATCCAGGATAGCAACGGTGAAAATAAGATTTTACGAAGGGCTTCTTCAGCTTCTCCACTAACCGTTGTTTGGGCTTTCACTATAAGAGCACGTATGACGCATACTACTCAGCTGGCTACTTTTGCAGTACGACGACTGGAGGCTGCGCGGGCTCATATTTGGCACCCGAAACCAATAGGTCCTATCGTTGCCACTTTTATTCTAAAvaIPfoISalIBsoBIBssSIBssSαIHincIIM24L25P26E27E28V29E30E31V32I33G34N35K36P37E38S39D40I41L42V43H44T45A46Y47D48E49S50T51D52E53N54V55M56L57L58T59S60D61A62P63E64Y65K66P67W68A69L70V71I72Q73D74S75N76G77E78N79K80I814483-4737ugi4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAAATGTTAtaaggcactcctcagcaaatataatgaccctcttgataacccaagagggcattttttaatgcccAtggcgtcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaTTTTACAATattccgtgaggagtcgtttatattactgggagaactattgggttctcccgtaaaaaattacgggTaccgcagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttNcoIBbvCIBsaHIK82M83L84*854483-4737aph (KanR)tetA/orfL bidirectional terminatorChen, Voigt e...th 0.84±0.56Chen, Voigt ...th 1.11±0.3ugi4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaataBstYIPflMIaph (KanR)4,9204,9404,9604,9805,0005,0205,0405,060
    gcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacgBtsIPvuIAsiSIBsmBIBtsαIEsp3Iaph (KanR)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagaAvaIBtsISmaIXmaIBsoBIBtsαIEcoNITspMIaph (KanR)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgcattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcClaINruIBspDIaph (KanR)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaAgagaaaccTacggatcggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactTctctttggAtgcctagccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgagPfoISacIIp15A Ori RNAIIp mutantRNA IIaph (KanR)US...25,6205,6405,6605,6805,7005,7205,7405,760
    cgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccagcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtAgeIEaeIBssSIBssSαIp15A Ori RNAIIp mutantRNA II5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatSmlIAlwNIBpuEIp15A Ori RNAIIp mutantRNA II5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagaTattacgcgcagaccaaaaccagaacttcagtacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctAtaatgcgcgtctggttttgBpuEIBstBIp15A Ori RNAIIp mutantRNA IIRNA IRNA I Promoter (0.96)RNA II Pro...er (0.92)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gatctcaagaagatcatcttattaaggagccccatacgatataagaggacatccggtctagagttcttctagtagaataattcctcggggtatgctatattctcctgtaggccaAhdISmlIp15A ...tantRNA ...92)6,3206,340
    • Plasmid
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    Editing is disabled because sequence edits have been locked.
    6357 bp450050005500600050010001500dCas9+1 TSSpRNAII G>T364-369259-363370-4470rrnB1 rho-independent transcriptional terminator,T-rich,stem loop,loop,+22from XTEN linker,BlpIBsmBIEsp3I,BstZ17I,BmgBI,SfcINdeI,SwaIBsgIBsrDI,BlpI,BsrDIBstAPIBsaXIHindIIINheI,BmtIFspItetA/orfL bidirectional terminator,+5PflMIBstYIaph (KanR),BsmBI,Esp3I,AsiSI,PvuIBtsI,BtsαI,EcoNI,AvaI,BsoBI,TspMI,XmaI,SmaIBtsI,BtsαIBspDIClaI,NruIUSER junction 22,prim transcript,PfoI,SacIIRNA II,p15A Ori RNAIIp mutant,BssSI,BssSαI,AgeI,EaeIRNA I Promoter (0.96),-35 -35 signal,-10 -10 signal,RNA I,RNA II Promoter (0.92),+9

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