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pDCAFAB
  • Sequence Map
    Editing disabled on read-only sequence.
    tttacggctagctcagtcctaggtacaatgctagctactagagaaatcaaattaaggaggtaagataaatggaacaggcaatcattaatgatgaacgggagtatcttcgccatttctggcatcccgtatgtactgtaactgagcttgaaaaggcgcatccttccagcctcggcccaaatgccgatcgagtcaggatccatgttacgatcgatgatctctttagtttaattcctccattctatttaccttgtccgttagtaattactacttgccctcatagaagcggtaaagaccgtagggcatacatgacattgactcgaacttttccgcgtaggaaggtcggagccgggAseIBaeIAvrIIME2QA4II6ND8ER10EY12LR14HF16WH18PV20CT22VT24EL26EK28AH30PS32SL34GP3669-1124ndmA CDSndmA geneJ23110derived f...5105 RBSiGEM15-01C20406080100120140160
    cctggccgttaagctgctgaatgaacagctcgttgtcgccaagctaggcgatgagtacgtcgcgatgcgtgatagatgcgctcatcgatcagctaagctttccttgggtacagtaagtggaaaccgcctacagtgcccctatcacggatggcaatatgatacgcatggcgcttgcggaccggcaattcgacgacttacttgtcgagcaacagcggttcgatccgctactcatgcagcgctacgcactatctacgcgagtagctagtcgattcgaaaggaacccatgtcattcacctttggcggatgtcacggggatagtgcctaccgttatactatgcgtaccgcgaacgBlpINruILA38VK40LL42NE44QL46VV48AK50LG52DE54YV56AM58RD60RC62AH64RS66AK68LS70LG72TV74SG76NR78LQ80CP82YH84GW86QY88DT90HG92AC9469-1124ndmA CDSndmA gene180200220240260280300320340
    cagctcgtaccagcgtgccccaacagcccaatacccaacaaggctaaagttgatcgcttcgattgcgaagaacgctatggattgatttggattcgattggactctagttttgactgcactgaaattccctactttagtgcagccaacgatcctaggttgcgtattgttatacaaggtcgagcatggtcgcacggggttgtcgggttatgggttgttccgatttcaactagcgaagctaacgcttcttgcgatacctaactaaacctaagctaacctgagatcaaaactgacgtgactttaagggatgaaatcacgtcggttgctaggatccaacgcataacaatatgttcAvrIIQL96VP98AC100PN102SP104IP106NK108AK110VD112RF114DC116EE118RY120GL122IW124IR126LD128SS130FD132CT134EI136PY138FS140AA142ND144PR146LR148IV150IQ15269-1124ndmA CDSndmA gene360380400420440460480500520
    aaccttactggtgggatgcgactgcggaacgtagatgggaaaattttacagatttttctcactttgcattcattcacccaggcacgcttttcgatccaaataatgctgaacctccaattgttccgatggatcgattcaatggtcagtttcggtttgtctacgacacgccagaagattggaatgaccaccctacgctgacgccttgcatctacccttttaaaatgtctaaaaagagtgaaacgtaagtaagtgggtccgtgcgaaaagctaggtttattacgacttggaggttaacaaggctacctagctaagttaccagtcaaagccaaacagatgctgtgcggtcttctBcgIMfeIEP154YW156WD158AT160AE162RR164WE166NF168TD170FS172HF174AF176IH178PG180TL182FD184PN186NA188EP190PI192VP194MD196RF198NG200QF202RF204VY206DT208PE210D69-1124ndmA CDSndmA geneiGEM15-03CiGEM15-02C540560580600620640660680700
    tatggctgtcccaaatcaggctccaattggttcattttcgtatacttgcagcatgccgtttgctattaaccttgaagtatccaaatactccagcagttcgctgcatgtgttattcaatgtgtcatgtccggtagacagccacaccacgaaaaactttctgatcttcgctagggagataccgacagggtttagtccgaggttaaccaagtaaaagcatatgaacgtcgtacggcaaacgataattggaacttcataggtttatgaggtcgtcaagcgacgtacacaataagttacacagtacaggccatctgtcggtgtggtgctttttgaaagactagaagcgatccctcMfeISphIBstZ17IM212AV214PN216QA218PI220GS222FS224YT226CS228MP230FA232IN234LE236VS238KY240SS242SS244LH246VL248FN250VS252CP254VD256SH258TT260KN262FL264IF266AR268E69-1124ndmA CDSndmA gene720740760780800820840860
    caatcggacgactcggattatctgcacattgcatttaatgatctcgtcttcgctgaagacaaaccagtaattgagtcccaatggcctaaagacgcgccagcagatgaggtctcagtagtcgcagataaggtatcgatacaatatagaaaatggctgcgggaactaaaagaagctcgttagcctgctgagcctaatagacgtgtaacgtaaattactagagcagaagcgacttctgtttggtcattaactcagggttaccggatttctgcgcggtcgtctactccagagtcatcagcgtctattccatagctatgttatatcttttaccgacgcccttgattttcttcgagBsaIQ270SD272DS274DY276LH278IA280FN282DL284VF286AE288DK290PV292IE294SQ296WP298KD300AP302AD304EV306SV308VA310DK312VS314IQ316YR318KW320LR322EL324KE326A69-1124ndmA CDSndmA gene8809009209409609801,0001,0201,040
    ataaagaaggttcacaagccttccgaagtgctttgttagacccagtcattgaaagcgataggagctacatataatactagagtcacacaggaaagtatactagatgaaagaacagctcaagccgctgctagaagacaagacttaccttcgccacttctggcatcccgtgtgtacctatttcttccaagtgttcggaaggcttcacgaaacaatctgggtcagtaactttcgctatcctcgatgtatattatgatctcagtgtgtcctttcatatgatctactttcttgtcgagttcggcgacgatcttctgttctgaatggaagcggtgaagaccgtagggcacacatggBmrIPflFIBstZ17ITth111IH328KE330GS332QA334FR336SA338LL340DP342VI344ES346DR348SY350I*35269-1124MK2EQ4LK6PL8LE10DK12TY14LR16HF18WH20PV22CT241154-2221iGEM15-04CiGEM15-26CndmA CDSndmA genendmB CDSndmB geneBBa_...0011,0601,0801,1001,1201,1401,1601,1801,2001,220
    cttaatgagttcgaacgcgccaacgccagtgggcacggccccatgggcgtcaccttgctaggcgagaaattggtgttggccaggttaaattcaaagatcattgcggctgctgaccgatgtgctcatcgatcggcacagctctccatcggccgcgtttgcagcaacgccggaaagggaattactcaagcttgcgcggttgcggtcacccgtgccggggtacccgcagtggaacgatccgctctttaaccacaaccggtccaatttaagtttctagtaacgccgacgactggctacacgagtagctagccgtgtcgagaggtagccggcgcaaacgtcgttgcggcctttccAjuIBglINcoIPvuIBstBICspCILN26EF28ER30AN32AS34GH36GP38MG40VT42LL44GE46KL48VL50AR52LN54SK56II58AA60AD62RC64AH66RS68AQ70LS72IG74RV76CS78NA80GK821154-2221iGEM15-04CiGEM15-26CndmB CDSndmB geneiGEM15C-07iGEM15C-061,2401,2601,2801,3001,3201,3401,3601,3801,400
    actatctcgaatgcccgtatcacggctggcgctacgatgaggctggggcctgtcaactgatccctgcttgccctgacaaaagcatctctcctcgggccaagatttcctcattcgattgtgaggtgaaatacgacatcgtgtgggtacggctggataacagcttcgattgcactcatgatagagcttacgggcatagtgccgaccgcgatgctactccgaccccggacagttgactagggacgaacgggactgttttcgtagagaggagcccggttctaaaggagtaagctaacactccactttatgctgtagcacacccatgccgacctattgtcgaagctaacgtgagtEcoO109IDY84LE86CP88YH90GW92RY94DE96AG98AC100QL102IP104AC106PD108KS110IS112PR114AK116IS118SF120DC122EV124KY126DI128VW130VR132LD134NS136FD138CT140Q1154-2221iGEM15-04CiGEM15-26CndmB CDSndmB gene1,4201,4401,4601,4801,5001,5201,5401,560
    gattccatacctcagcgatttcgataatcccgacatgcaggtaatcgttgccgattcgtatatttgggagactgttgccgagcggcggtgggagaactttacagatttttcgcactttgccttcgtacacccagggacgctctatgatccgtttttcgctagccacccaactgttctaaggtatggagtcgctaaagctattagggctgtacgtccattagcaacggctaagcatataaaccctctgacaacggctcgccgccaccctcttgaaatgtctaaaaagcgtgaaacggaagcatgtgggtccctgcgagatactaggcaaaaagcgatcggtgggttgacaaPasIBbvCIBfuAIBspMIBsrBII142PY144LS146DF148DN150PD152MQ154VI156VA158DS160YI162WE164TV166AE168RR170WE172NF174TD176FS178HF180AF182VH184PG186TL188YD190PF192FA194SH196PT198V1154-2221iGEM15-04CiGEM15-26CndmB CDSndmB gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tacgtgaatcgcgttgatggtgagttgcaattcaaactcgctccgccgcgtgaaatgaaaggcatcccgccagaagcaccgatgggtgacttcacctaccgctgcacaatgccgtattcagtaaatcttgaaatcaaattgtggaaagatgactcacgtttcgttctttggactaatgcacttagcgcaactaccactcaacgttaagtttgagcgaggcggcgcactttactttccgtagggcggtcttcgtggctacccactgaagtggatggcgacgtgttacggcataagtcatttagaactttagtttaacacctttctactgagtgcaaagcaagaaacctgatBsaAIY200VN202RV204DG206EL208QF210KL212AP214PR216EM218KG220IP222PE224AP226MG228DF230TY232RC234TM236PY238SV240NL242EI244KL246WK248DD250SR252FV254LW256T1154-2221iGEM15-04CiGEM15-26CndmB CDSndmB gene1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ccgctagccctgtcgataacaagtcttgccggaattttatgattattgtgcgtgagaaggataatcaacctgatcatatgcacctggctttccagaagcgggtgcttgacgaagaccagcctgttatcgaatcgcaatggcctctcgaaatacagacctcggaagtctccgttgcggcgatcgggacagctattgttcagaacggccttaaaatactaataacacgcactcttcctattagttggactagtatacgtggaccgaaaggtcttcgcccacgaactgcttctggtcggacaatagcttagcgttaccggagagctttatgtctggagccttcagaggcaacgBclINdeIT258AS260PV262DN264KS266CR268NF270MI272IV274RE276KD278NQ280PD282HM284HL286AF288QK290RV292LD294ED296QP298VI300ES302QW304PL306EI308QT310SE312VS314VA3161154-2221iGEM15-04CiGEM15-26CndmB CDSndmB gene1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aaccgataaaatttccgtccagttccgcaaatggcataaagagctatctctgtcagccgttgaaggacgggaggcgttccgcgattccgtcttgacgaatgttattgaagaagaacagtaatactagagtcacacaggaaagTACTAGatgaacaaacttgacgtcaaccagtggttggctattttaaaggcaggtcaaggcgtttaccgtatttctcgatagagacagtcggcaacttcctgccctccgcaaggcgctaaggcagaactgcttacaataacttcttcttgtcattatgatctcagtgtgtcctttcATGATCtacttgtttgaactgcagttggtcaccMN2KL4DV6NQ8W2249-4015TD318KI320SV322QF324RK326WH328KE330LS332LS334AV336EG338RE340AF342RD344SV346LT348NV350IE352EE354Q*3561154-2221iGEM15-04CiGEM15-26CndmB CDSndmB genendmD CDSndmD geneBBa...322,1202,1402,1602,1802,2002,2202,2402,260
    tttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcaaaggataacgatggtgacttctagagggcttcgcggtacagaaagtacggtgcaacaaccccgttctttaccggtagaccgcgctactgagaccaagtcaattacgaaccctcttggcgacgggcgtatctcctaacgccaactgtgacccacgattatggccattgctcaacgAgeIBmrIBglIIBstXIDraIIIF10PI12AT14TE16DL18PK20RH22VF24HA26TL28LG30QE32MA34IW36RD38DS40GS42VN44AW46EN48RC50PH52RG54LR56LT58LG60AN62TG64NE66L2249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatccacagtcatagtacctacctgaatactttcgccctgaccaccgacgtgaaaacagggtcgggtagcgctacgtggtgggggtttacggcgcgcccaattatgaaaaggacaggcgcttttcgtgccgaaatagacctgctgtaatccagtcggcggtcctctcgggtaaagttaAhdINruIR68CQ70YH72GW74TY76ES78GT80GG82CT84FV86PA88HR90DA92PP94PN96AA98RV100NT102FP104VR106EK108HG110FI112WT114TL116GQ118PP120GE122PI124SI1262249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgaggagctactgcgagtcgaacatttgcgacatttttgtttagacgtatcgcatcaatatctacgactataactgcctcaacagtcgcaggatgcagtcttagaaagtcgcaagtagctacacaaaccacgccggtcgcgacttctagacgtaaactttaggtacgacgttctagctAfeIBlpIDrdIBglIILD128DA130QL132VN134AV136KT138NL140HS142VV144ID146AD148ID150GV152VS154VL156RQ158NL160SA162FI164DV166FG168AA170SA172ED174LH176LK178SM180LQ182DR1842249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagccctaagaccattgttctagtccgagataacgataagtaaaaatatacgtcgcgagttggtaatcatttacgcaacatgtacgcgttcatgaatgaggccctgcaggtcctatgcttgaagttttcttgatgagcatacggtacttgcaataggcgtcccgtcgtcttcgacatcAclIAfeIBsrGIGI186LV188TR190SG192SI194AI196HF198YM200QR202ST204IS206KC208VV210HA212QV214LT216PG218RP220GY222EL224QK226NY228SY230AM232NV234IR236RA238AE240AV2422249-4015iGEM15-04CiGEM15-26CndmD CDSndmD geneiGEM15-24C2,8202,8402,8602,8802,9002,9202,9402,960
    ctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactgatggctgaactaatcgtaatgtctatagtcgctagtctgatagcttttcgaacttcagcaatctcttttgtactgattccgaggaggttgggtgatatagacgcttcaccaatgcgcatgagttctctgtccactataattgagtatgaccgacttcggatagccgatgggtgaMluIBsaXIEcoRVAflIIIAT244DL246IS248IT250DI252SD254QT256IE258KL260EV262VR264EN266MT268KA270PP272TH274YI276CE278VV280TR282TQ284ET286GD288IN290SY292WL294KP296IG298YP300L2249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    accagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgtggtcgtaagtcaggtccctacgtgtagtcgtagtgttgcggcctcccatcgtaagctgttataagggagcacttgcccggactgctcgcacttaggaagtagtagccacacttctttctctaagtcagggcaccgccgaggtctagttacgtgcttctacacttccaaccttgcBsaBIP302AF304SP306GM308HI310SI312TT314PE316GS318IR320QY322SL324VN326GP328DE330RE332SF334II336GV338KK340EI342QS344RG346GS348RS350MH352ED354VK356VG358T2249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    caactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttcgttgattttcaatgtgaaggctccttgccaaaaggtgagcaggtttggtcttttgtgggctaagagcatcgtccaccgtagccatagtggggttaaaacacataccgtgtccgagacctagtcgttccaagtagcatactttatgtaataaaacgagcacgtaaactcgtacaagBfuAIBspMIPaqCIQ360LK362VT364LP366RN368GF370PL372VQ374TR376KH378PI380LV382AG384GI386GI388TP390IL392CM394AQ396AL398DQ400QG402SS404YE406IH408YF410AR412AF414EH416V2249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctctgtaaggtcctagctgactgacgcaacccgctagcaaacttacacgtagaaccggagccgggtctgctctgatctcgttttgaacggctgtagtacctctaagtattgcgggttctgcatctacaaatgtgaacgccgggcgttggttactagctttgacatagacgacagcgagaPvuIBstAPIP418FQ420DR422LT424AL426GD428RL430NV432HL434GL436GP438DE440TR442AK444LA446DI448ME450IH452NA454QD456VD458VY460TC462GP464QP466MI468ET470VS472AV474AL4762249-4015iGEM15-04CiGEM15-26CndmD CDSndmD geneiGEM15-25C3,5203,5403,5603,5803,6003,6203,6403,660
    tgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaaacgagtaccgtagcgacttctcaggtaggctaaacttaaaaagtcatttttcttgctacaagggcaaagactacttcttatactccaactcgagtttttttgaccagtttataagtgacagagcggaccgagatgcaacaacgttcgaacaaacctgttgctacaagcatagcttEarISacIEco53kIAH478GI480AE482ES484IR486FE488FF490SK492KN494DV496PV498SD500EE502YE504VE506LK508KT510GQ512IF514TV516SP518GS520TL522LQ524AC526LD528ND530VR532IE5342249-4015iGEM15-04CiGEM15-26CndmD CDSndmD gene3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcgaagaacactcgtcccacatacgccctgaacatattgaggtcagcataggccgctagagctcgtagtactgtgaatggaaagattctttctttccctttcgccattcacctagtacggcacacaaagcgcgacgttcaggtttttttagcaagagctagacactccggcccgtaBarIAS536CE538QG540VC542GT544CI546TP548VV550SG552DL554EH556HD558TY560LS562KK564ER566ES568GK570WI572MP574CV576SR578CK580SK582KI584VL586DL588*2249-4015iGEM15-04CiGEM15-26CndmD CDSndmD geneEQ_NdmD_pSB1C3_rev3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaagtgggtgccatacctgctcgacatatttgatctctttctcctctttatgatctactagtgcgaaatactgatacttgagagccccttaacgatgttccacgcctacgaggaaaggtaggacttcgatggcatgtgctatctctgtcaccttaaaaagggcaggtcccttgtatttBarIBclIPflMIMI2TL4YD6YE8LS10GN12CY14KV16RM18LL20SI22LK24LP26YT28IE30TV32EF34FP36SR38EH40K4078-4752iGEM15-04CiGEM15-26CEQ_gst9_1 (gBLOCK)gst9 CDSgst9 geneEQ_NdmD_pSB1C3_revEQ_pSB...b_forBBa...BS4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcaccggccttcctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggcagtggccggaaggacgcgtagttaggtgagccggtcaacggccataacgcgctactactaaagcacaacgcgctacgggtccggtaggaccaaatggaccgcttgtttatgctgtggtcaaactggaccataggctgtgtacgcgggcggtaggagccggtccgtttatacaccgEcoNISexAIS42PA44FL46RI48NP50LG52QL54PV56LR58DD60DF62VL64RD66AQ68AI70LV72YL74AN76KY78DT80SL82TW84YP86TH88AP90AI92LG94QA96NM98W4078-4752iGEM15-04CiGEM15-26CEQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene4,2204,2404,2604,2804,3004,3204,3404,360
    tcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgcagcgcaaacggctaccaaactggccgagctaaaggcgacgtgcaaacgtactaaacaaaaaaatgctaaagttacgtctcgttacggcacgggcgcgcgtgcttgatgacgcgtatgacctgcttgtggacacccgccgccttgttttacctgtagttaccgatacaggccggcgBcgIBssHIIL100AF102AD104GL106TG108SI110SA112AR114LH116DL118FF120YD122FN124AE126QC128RA130RA132HE134LL136RI138LD140EH142LW144AA146EQ148NG150HQ152WL154CP156AA1584078-4752iGEM15-04CiGEM15-26Cgst9 CDSgst9 geneEQ_gst9_1 (gBLOCK)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgagccggccgcgtgggtggtagcgcctagaacggacgaagggcatatagcgtaacagcctacttcggccgcaaagtgatgagctgatgggccgccatgctgcgaccgacctggcacagtttgcgtaggggcctaagtggcagtacagcccctaaaaaggttggagtcgaaggaagctcggccggAjuIBplINaeINaeIAvaIIBbvCIPflFINgoMIVNgoMIVTth111IAP160TI162AD164LA166CF168PY170IA172LS174DE176AG178VS180LL182DY184PA186VR188RW190LD192RV194KR196IP198GF200TV202MS204GI206FP208TS210AS212FE214PA2164078-4752iGEM15-04CiGEM15-26Cgst9 CDSgst9 gene4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tcacttgcggaagaaacctgtcaataatactagTAGCGGCCGCTGCagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgagtgaacgccttctttggacagttattatgatcATCGCCGGCGACGtcaggccgttttttcccgttccacagtggtgggacgggaaaaagaaattttggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgcBplINotIPstISpeIBsrBISL218AE220ET222CQ224*4078-4752E. coli His Operon TerminatoriGEM15-26CiGEM15-04CiGEM15C-12gst9 CDSgst9 geneBioBrick Suffix4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaacatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttDrdIPciIAflIIIrep (pMB1)VR Primer4,9204,9404,9604,9805,0005,0205,0405,060
    gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccarep (pMB1)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtApaLIrep (pMB1)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaactcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtcttrep (pMB1)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgtttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaacrep (pMB1)5,6205,6405,6605,6805,7005,7205,7405,760
    gtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaacagactgtcgagctccgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattSacIEcoRVPspXIEco53kIT0 TerminatorChloramphenicol Resistance5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ttcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtcNcoIPasIChloramphenicol Resistance5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaaccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgAclIBsmBIEsp3IPflMIChloramphenicol Resistance6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttatattcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatBspEIPvuIIChloramphenicol Resistance6,3206,3406,3606,3806,4006,4206,4406,460
    ggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcChloramphenicol Resistance6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagaaccttggagaatgcacgggctagttgagctcacggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgaaagcgattcctactaaagaccttaagcgccggcgaagatctcNotIXbaIBsaAIEcoRIPspXIStem LoopVF2 PrimeriGEM15-01CPrefix6,6606,6806,7006,7206,7406,7606,7806,8006,820
    • Plasmid
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    Editing disabled on read-only sequence.
    6822 bp500055006000650050010001500iGEM15-26CiGEM15-04CndmB CDSndmB geneiGEM15C-07iGEM15C-06point mutationndmA CDSndmA geneiGEM15-01C69-11241154-2221

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    6822