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pDCAFBC
  • Sequence Map
    Editing disabled on read-only sequence.
    actcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatctgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagNcoIChloramphenicol Resistance20406080100120140160
    aaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagttttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaAclIPasIBsmBIEsp3IPflMIChloramphenicol Resistance180200220240260280300320340
    tgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataBspEIChloramphenicol Resistance360380400420440460480500520
    ccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgaggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactPvuIIChloramphenicol Resistance540560580600620640660680700
    tcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctgGAAagaataaagtaataccactttcaaccttggagaatgcacgggctagttgagctcacggtggactgcagattctttggtaataatagtactgtaattggatatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgaaagcgattcctactaaagacCTTBsaAIEcoRIPspXIStem LoopVF2 Primer720740760780800820840860
    TTCGCGGCCGCTTCTAGAGtttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtatactagatgaaagaacagctcaagccgctgctagaagacaagacttaccttcgccacttctggcatcccgtgtgtacccttaatgagttcgaacgcgcAAGCGCCGGCGAAGATCTCaaatgccgatcgagtcaggatccatgttacgatcgatgatctcagtgtgtcctttcatatgatctactttcttgtcgagttcggcgacgatcttctgttctgaatggaagcggtgaagaccgtagggcacacatgggaattactcaagcttgcgcgNotIXbaIAvrIIBstBICspCIBstZ17IMK2EQ4LK6PL8LE10DK12TY14LR16HF18WH20PV22CT24LN26EF28ER30A959-2026iGEM15C-05ndmB CDSndmB geneJ23110iGEM15C-06iGEM15C-07PrefixBBa_...0018809009209409609801,0001,0201,040
    caacgccagtgggcacggccccatgggcgtcaccttgctaggcgagaaattggtgttggccaggttaaattcaaagatcattgcggctgctgaccgatgtgctcatcgatcggcacagctctccatcggccgcgtttgcagcaacgccggaaaggactatctcgaatgcccgtatgttgcggtcacccgtgccggggtacccgcagtggaacgatccgctctttaaccacaaccggtccaatttaagtttctagtaacgccgacgactggctacacgagtagctagccgtgtcgagaggtagccggcgcaaacgtcgttgcggcctttcctgatagagcttacgggcataAjuIBglIClaINcoIPvuIBspDIN32AS34GH36GP38MG40VT42LL44GE46KL48VL50AR52LN54SK56II58AA60AD62RC64AH66RS68AQ70LS72IG74RV76CS78NA80GK82DY84LE86CP88Y959-2026ndmB CDSndmB gene1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cacggctggcgctacgatgaggctggggcctgtcaactgatccctgcttgccctgacaaaagcatctctcctcgggccaagatttcctcattcgattgtgaggtgaaatacgacatcgtgtgggtacggctggataacagcttcgattgcactcagattccatacctcagcgattgtgccgaccgcgatgctactccgaccccggacagttgactagggacgaacgggactgttttcgtagagaggagcccggttctaaaggagtaagctaacactccactttatgctgtagcacacccatgccgacctattgtcgaagctaacgtgagtctaaggtatggagtcgctaaBbvCIEcoO109IH90GW92RY94DE96AG98AC100QL102IP104AC106PD108KS110IS112PR114AK116IS118SF120DC122EV124KY126DI128VW130VR132LD134NS136FD138CT140QI142PY144LS146D959-2026ndmB CDSndmB gene1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tcgataatcccgacatgcaggtaatcgttgccgattcgtatatttgggagactgttgccgagcggcggtgggagaactttacagatttttcgcactttgccttcgtacacccagggacgctctatgatccgtttttcgctagccacccaactgtttacgtgaatcgcgttgatggagctattagggctgtacgtccattagcaacggctaagcatataaaccctctgacaacggctcgccgccaccctcttgaaatgtctaaaaagcgtgaaacggaagcatgtgggtccctgcgagatactaggcaaaaagcgatcggtgggttgacaaatgcacttagcgcaactaccPasIBfuAIBsaAIBspMIBsrBIF148DN150PD152MQ154VI156VA158DS160YI162WE164TV166AE168RR170WE172NF174TD176FS178HF180AF182VH184PG186TL188YD190PF192FA194SH196PT198VY200VN202RV204DG206959-2026ndmB CDSndmB gene1,4201,4401,4601,4801,5001,5201,5401,560
    tgagttgcaattcaaactcgctccgccgcgtgaaatgaaaggcatcccgccagaagcaccgatgggtgacttcacctaccgctgcacaatgccgtattcagtaaatcttgaaatcaaattgtggaaagatgactcacgtttcgttctttggactaccgctagccctgtcgataacactcaacgttaagtttgagcgaggcggcgcactttactttccgtagggcggtcttcgtggctacccactgaagtggatggcgacgtgttacggcataagtcatttagaactttagtttaacacctttctactgagtgcaaagcaagaaacctgatggcgatcgggacagctattgEL208QF210KL212AP214PR216EM218KG220IP222PE224AP226MG228DF230TY232RC234TM236PY238SV240NL242EI244KL246WK248DD250SR252FV254LW256TT258AS260PV262DN264959-2026ndmB CDSndmB gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aagtcttgccggaattttatgattattgtgcgtgagaaggataatcaacctgatcatatgcacctggctttccagaagcgggtgcttgacgaagaccagcctgttatcgaatcgcaatggcctctcgaaatacagacctcggaagtctccgttgcaaccgataaaatttccgtccttcagaacggccttaaaatactaataacacgcactcttcctattagttggactagtatacgtggaccgaaaggtcttcgcccacgaactgcttctggtcggacaatagcttagcgttaccggagagctttatgtctggagccttcagaggcaacgttggctattttaaaggcaggBclINdeIKS266CR268NF270MI272IV274RE276KD278NQ280PD282HM284HL286AF288QK290RV292LD294ED296QP298VI300ES302QW304PL306EI308QT310SE312VS314VA316TD318KI320SV322959-2026ndmB CDSndmB gene1,7601,7801,8001,8201,8401,8601,8801,9001,920
    agttccgcaaatggcataaagagctatctctgtcagccgttgaaggacgggaggcgttccgcgattccgtcttgacgaatgttattgaagaagaacagtaatactagagATTAAAGAGGAGAAATACTAGatgtctactgaccaagtaatttttaacgactggcatccagtcgcttcaaggcgtttaccgtatttctcgatagagacagtcggcaacttcctgccctccgcaaggcgctaaggcagaactgcttacaataacttcttcttgtcattatgatctcTAATTTCTCCTCTTTATGATCtacagatgactggttcattaaaaattgctgaccgtaggtcagcgaQF324RK326WH328KE330LS332LS334AV336EG338RE340AF342RD344SV346LT348NV350IE352EE354Q*356959-2026MS2TD4QV6IF8ND10WH12PV14A2056-2910ndmB CDSndmB genendmC CDSndmC geneBBa_B00301,9401,9601,9802,0002,0202,0402,0602,0802,100
    gctttggaagatgtatctctcgataaacgataccgctgtcgactactaggtcgtacagttagttatgtgaaaacatctgatgctgttaatgctcattgggaagaaagtgcagatgaaattaagaccatccgcgctaaagaaatctatggtcttctgtggctctcctttgccgacacgaaaccttctacatagagagctatttgctatggcgacagctgatgatccagcatgtcaatcaatacacttttgtagactacgacaattacgagtaacccttctttcacgtctactttaattctggtaggcgcgatttctttagataccagaagacaccgagaggaaacggctgtBaeISalIA16LE18DV20SL22DK24RY26RC28RL30LG32RT34VS36YV38KT40SD42AV44NA46HW48EE50SA52DE54IK56TI58RA60KE62IY64GL66LW68LS70FA72D2056-2910ndmC CDSndmC gene2,1202,1402,1602,1802,2002,2202,2402,260
    aacccagtgagatgttcgatattgcagagttcaaggaacctgatcgccgaatcgtcagcgctggatctgtgcgcgtaaatgtctcaggactgcgtgctatcgaaaactttctggacatggctcatttcccttttgttcatacggatattttgggtgcagagccactgacggaagtttgggtcactctacaagctataacgtctcaagttccttggactagcggcttagcagtcgcgacctagacacgcgcatttacagagtcctgacgcacgatagcttttgaaagacctgtaccgagtaaagggaaaacaagtatgcctataaaacccacgtctcggtgactgccttcaXmnIK74PS76EM78FD80IA82EF84KE86PD88RR90IV92SA94GS96VR98VN100VS102GL104RA106IE108NF110LD112MA114HF116PF118VH120TD122IL124GA126EP128LT130EV1322056-2910ndmC CDSndmC gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgagccgtataacgtcaactatgatgaaactgttgatgagatcttcgctactgagtgtaagttcccgcaacctaaaggctcagctactgctgtcgagccaattgatatgcagtatatataccgcattacacggccatattccgccattttatataaaacttgcccgcccgaaccgactcggcatattgcagttgatactactttgacaactactctagaagcgatgactcacattcaagggcgttggatttccgagtcgatgacgacagctcggttaactatacgtcatatatatggcgtaatgtgccggtataaggcggtaaaatatattttgaacgggcgggcttggcBlpIMfeIEP134YN136VN138YD140ET142VD144EI146FA148TE150CK152FP154QP156KG158SA160TA162VE164PI166DM168QY170IY172RI174TR176PY178SA180IL182YK184TC186PP188EP1902056-2910ndmC CDSndmC gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    catagatgggatgccctcggtctgtttattcaacctgtagacgaagattggtgcatagcgcatacaattatgtgctatgtggatgacgttaactcagatcaacaacttcgccatttccagcagacgatttttggccaggacttgatgattcttatcaaccaagttccaaagcgtcgtatctaccctacgggagccagacaaataagttggacatctgcttctaaccacgtatcgcgtatgttaatacacgatacacctactgcaattgagtctagttgttgaagcggtaaaggtcgtctgctaaaaaccggtcctgaactactaagaatagttggttcaaggtttcgcagHpaIBsaBIHR192WD194AL196GL198FI200QP202VD204ED206WC208IA210HT212IM214CY216VD218DV220NS222DQ224QL226RH228FQ230QT232IF234GQ236DL238MI240LI242NQ244VP246KR2482056-2910ndmC CDSndmC gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ttcccctggctgcaagtcgagagagcccagtccgggccgatgtgcttgcaacagcttatcgtcgctggctgcgtgagaaaggtgtgcaatatggtgctctgcgggactaatactagagtcacacaggaaagTACTAGatgaacaaacttgacgtcaaccagtggtttcctattgcaaggggaccgacgttcagctctctcgggtcaggcccggctacacgaacgttgtcgaatagcagcgaccgacgcactctttccacacgttataccacgagacgccctgattatgatctcagtgtgtcctttcATGATCtacttgtttgaactgcagttggtcaccaaaggataacgBcgIMN2KL4DV6NQ8WF10PI12A2938-4704LP250LA252AS254RE256SP258VR260AD262VL264AT266AY268RR270WL272RE274KG276VQ278YG280AL282RD284*2056-2910ndmC CDSndmC genendmD CDSndmD geneEQ_NdmC_revBBa...322,8202,8402,8602,8802,9002,9202,9402,960
    taccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatatggtgacttctagagggcttcgcggtacagaaagtacggtgcaacaaccccgttctttaccggtagaccgcgctactgagaccaagtcaattacgaaccctcttggcgacgggcgtatctcctaacgccaactgtgacccacgattatggccattgctcaacgccacagtcataAgeIBstXIDraIIIT14TE16DL18PK20RH22VF24HA26TL28LG30QE32MA34IW36RD38DS40GS42VN44AW46EN48RC50PH52RG54LR56LT58LG60AN62TG64NE66LR68CQ70Y2938-4704ndmD CDSndmD gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    catggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacggtacctacctgaatactttcgccctgaccaccgacgtgaaaacagggtcgggtagcgctacgtggtgggggtttacggcgcgcccaattatgaaaaggacaggcgcttttcgtgccgaaatagacctgctgtaatccagtcggcggtcctctcgggtaaagttaggagctactgcAhdINruIH72GW74TY76ES78GT80GG82CT84FV86PA88HR90DA92PP94PN96AA98RV100NT102FP104VR106EK108HG110FI112WT114TL116GQ118PP120GE122PI124SI126LD128D2938-4704ndmD CDSndmD gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtgagtcgaacatttgcgacatttttgtttagacgtatcgcatcaatatctacgactataactgcctcaacagtcgcaggatgcagtcttagaaagtcgcaagtagctacacaaaccacgccggtcgcgacttctagacgtaaactttaggtacgacgttctagctccctaagaccaBlpIClaIBspDIA130QL132VN134AV136KT138NL140HS142VV144ID146AD148ID150GV152VS154VL156RQ158NL160SA162FI164DV166FG168AA170SA172ED174LH176LK178SM180LQ182DR184GI186LV1882938-4704ndmD CDSndmD gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgttgttctagtccgagataacgataagtaaaaatatacgtcgcgagttggtaatcatttacgcaacatgtacgcgttcatgaatgaggccctgcaggtcctatgcttgaagttttcttgatgagcatacggtacttgcaataggcgtcccgtcgtcttcgacatcgatggctgaacAclIBsrGITR190SG192SI194AI196HF198YM200QR202ST204IS206KC208VV210HA212QV214LT216PG218RP220GY222EL224QK226NY228SY230AM232NV234IR236RA238AE240AV242AT244DL2462938-4704ndmD CDSndmD geneiGEM15-24C3,5203,5403,5603,5803,6003,6203,6403,660
    attagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcataatcgtaatgtctatagtcgctagtctgatagcttttcgaacttcagcaatctcttttgtactgattccgaggaggttgggtgatatagacgcttcaccaatgcgcatgagttctctgtccactataattgagtatgaccgacttcggatagccgatgggtgatggtcgtaagtMluIBsaXIEcoRVAflIIIIS248IT250DI252SD254QT256IE258KL260EV262VR264EN266MT268KA270PP272TH274YI276CE278VV280TR282TQ284ET286GD288IN290SY292WL294KP296IG298YP300LP302AF3042938-4704ndmD CDSndmD gene3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagtcaggtccctacgtgtagtcgtagtgttgcggcctcccatcgtaagctgttataagggagcacttgcccggactgctcgcacttaggaagtagtagccacacttctttctctaagtcagggcaccgccgaggtctagttacgtgcttctacacttccaaccttgcgttgattttcaBsaBISP306GM308HI310SI312TT314PE316GS318IR320QY322SL324VN326GP328DE330RE332SF334II336GV338KK340EI342QS344RG346GS348RS350MH352ED354VK356VG358TQ360LK362V2938-4704ndmD CDSndmD gene3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatatgtgaaggctccttgccaaaaggtgagcaggtttggtcttttgtgggctaagagcatcgtccaccgtagccatagtggggttaaaacacataccgtgtccgagacctagtcgttccaagtagcatactttatgtaataaaacgagcacgtaaactcgtacaaggtaaggtcctaBfuAIBspMIPaqCIT364LP366RN368GF370PL372VQ374TR376KH378PI380LV382AG384GI386GI388TP390IL392CM394AQ396AL398DQ400QG402SS404YE406IH408YF410AR412AF414EH416VP418FQ420D2938-4704ndmD CDSndmD gene4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcagctgactgacgcaacccgctagcaaacttacacgtagaaccggagccgggtctgctctgatctcgttttgaacggctgtagtacctctaagtattgcgggttctgcatctacaaatgtgaacgccgggcgttggttactagctttgacatagacgacagcgagaacgagtaccgtPvuIBstAPIR422LT424AL426GD428RL430NV432HL434GL436GP438DE440TR442AK444LA446DI448ME450IH452NA454QD456VD458VY460TC462GP464QP466MI468ET470VS472AV474AL476AH478G2938-4704ndmD CDSndmD geneiGEM15-25C4,2204,2404,2604,2804,3004,3204,3404,360
    tcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgaagcgacttctcaggtaggctaaacttaaaaagtcatttttcttgctacaagggcaaagactacttcttatactccaactcgagtttttttgaccagtttataagtgacagagcggaccgagatgcaacaacgttcgaacaaacctgttgctacaagcatagcttcgaagaacactEarISacIEco53kII480AE482ES484IR486FE488FF490SK492KN494DV496PV498SD500EE502YE504VE506LK508KT510GQ512IF514TV516SP518GS520TL522LQ524AC526LD528ND530VR532IE534AS536CE5382938-4704ndmD CDSndmD gene4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtacgtcccacatacgccctgaacatattgaggtcagcataggccgctagagctcgtagtactgtgaatggaaagattctttctttccctttcgccattcacctagtacggcacacaaagcgcgacgttcaggtttttttagcaagagctagacactccggcccgtagtgggtgccatBarIPflMIQG540VC542GT544CI546TP548VV550SG552DL554EH556HD558TY560LS562KK564ER566ES568GK570WI572MP574CV576SR578CK580SK582KI584VL586DL588*2938-4704ndmD CDSndmD geneEQ_NdmD_pSB1C3_revEQ_pS..._forEQ_gs...OCK)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttacctgctcgacatatttgatctctttctcctctttatgatctactagtgcgaaatactgatacttgagagccccttaacgatgttccacgcctacgaggaaaggtaggacttcgatggcatgtgctatctctgtcaccttaaaaagggcaggtcccttgtatttagtggccggaaBarIBclIMI2TL4YD6YE8LS10GN12CY14KV16RM18LL20SI22LK24LP26YT28IE30TV32EF34FP36SR38EH40KS42PA44F4767-5441EQ_gst9_1 (gBLOCK)gst9 CDSgst9 geneEQ_Nd..._revBBa...BS4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccggacgcgtagttaggtgagccggtcaacggccataacgcgctactactaaagcacaacgcgctacgggtccggtaggaccaaatggaccgcttgtttatgctgtggtcaaactggaccataggctgtgtacgcgggcggtaggagccggtccgtttatacaccgagcgcaaacggEcoNISexAIL46RI48NP50LG52QL54PV56LR58DD60DF62VL64RD66AQ68AI70LV72YL74AN76KY78DT80SL82TW84YP86TH88AP90AI92LG94QA96NM98WL100AF102A4767-5441EQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene4,9204,9404,9604,9805,0005,0205,0405,060
    gatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccactaccaaactggccgagctaaaggcgacgtgcaaacgtactaaacaaaaaaatgctaaagttacgtctcgttacggcacgggcgcgcgtgcttgatgacgcgtatgacctgcttgtggacacccgccgccttgttttacctgtagttaccgatacaggccggcggcgtgggtggtBcgIBssHIID104GL106TG108SI110SA112AR114LH116DL118FF120YD122FN124AE126QC128RA130RA132HE134LL136RI138LD140EH142LW144AA146EQ148NG150HQ152WL154CP156AA158AP160T4767-5441gst9 CDSgst9 geneEQ_gst9_1 (gBLOCK)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgagccggcctcacttgcggaagcgcctagaacggacgaagggcatatagcgtaacagcctacttcggccgcaaagtgatgagctgatgggccgccatgctgcgaccgacctggcacagtttgcgtaggggcctaagtggcagtacagcccctaaaaaggttggagtcgaaggaagctcggccggagtgaacgcctAjuIBplINaeINaeIAvaIIBbvCIPflFINgoMIVNgoMIVTth111II162AD164LA166CF168PY170IA172LS174DE176AG178VS180LL182DY184PA186VR188RW190LD192RV194KR196IP198GF200TV202MS204GI206FP208TS210AS212FE214PA216SL218AE2204767-5441gst9 CDSgst9 gene5,2605,2805,3005,3205,3405,3605,3805,4005,420
    agaaacctgtcaataatactagtagcggccgctgcagatatgctccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatctctttggacagttattatgatcatcgccggcgacgtctatacgaggccgttttttcccgttccacagtggtgggacgggaaaaagaaattttggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagBplINotIPstISpeIBsrBIET222CQ224*4767-5441E. coli His Operon TerminatorSuffixgst9 CDSgst9 gene5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtcPciIAflIIIrep (pMB1)VR Primer5,6205,6405,6605,6805,7005,7205,7405,760
    aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccrep (pMB1)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaaApaLIrep (pMB1)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttrep (pMB1)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagacrep (pMB1)6,3206,3406,3606,3806,4006,4206,4406,460
    acagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgcctgtcgagctccgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctacctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggSacIEcoRVPspXIEco53kIT0 TerminatorChlora...tance6,4806,5006,5206,5406,5606,5806,6006,620
    • Plasmid
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    Editing disabled on read-only sequence.
    6623 bp450050005500600050010001500ndmB CDSndmB genegst9 geneiGEM15C-07iGEM15C-06point mutationORI4767-5441959-2026Chloramphenicol Resistance,NcoI,PasI,PflMI,BsmBI,Esp3IAclIBspEI,PvuIIVF2 Primer,BsaAI,PspXIEnd of Terminator,Stem Loop,Prefix,iGEM15C-05,J23110,EcoRI,NotI,+2BBa_K082001,BstZ17ICspCI,BstBI,NcoI,BglI,AjuIBspDI,ClaI,PvuIEcoO109IBbvCIBfuAI,BspMI,BsrBIPasIBsaAIEQ_gst9_1 (gBLOCK)SexAI,EcoNIgst9 CDS,BcgIBssHIINgoMIV,NaeIAvaII,PflFI,Tth111I,BbvCI,BplISuffix,NgoMIV,NaeI,AjuI,BplI,SpeI,NotI,PstIE. coli His Operon TerminatorBsrBIVR PrimerAflIII,PciIApaLIrep (pMB1)T0 Terminator,PspXI,Eco53kI,SacI,EcoRV

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    6623