Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
T181 Target (pJBL2148)
  • Sequence Map
    Editing disabled on read-only sequence.
    GAATTCTAAAGATCTTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTACATAAAGATATATATTTGGGTGAGCGATTCCTTAAACGAAATTGAGATTAAGGAGTCGCTCTTTTTTTTTTATGTATAAAAACAATCATGCAAATCATTCAAATCATTTGGAAAATCACGATTTCTTAAGATTTCTAGAAACTGTCGATCGAGTCAGGATCCATATTATGATCATGTATTTCTATATATAAACCCACTCGCTAAGGAATTTGCTTTAACTCTAATTCCTCAGCGAGAAAAAAAAAATACATATTTTTGTTAGTACGTTTAGTAAGTTTAGTAAACCTTTTAGTGCTAAABmtINheISpeIStyIAvrIIBglIIEcoRIT181 TargetPromoter_J2311920406080100120140160
    AGACAATTTTTCTAAAACCGGCTACTCTAATAGCCGGTTGTAAGGATCTAGGAGGAAGGATCTATGAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCCGTGGAGAGGGTGAAGGTGATGCTATCTGTTAAAAAGATTTTGGCCGATGAGATTATCGGCCAACATTCCTAGATCCTCCTTCCTAGATACTCGTTTCCTCTTCTTGAAAAGTGACCTCAACAGGGTTAAGAACAACTTAATCTACCACTACAATTACCCGTGTTTAAAAGACAGGCACCTCTCCCACTTCCACTACGATsfGFPT181 TargetRBS180200220240260280300320340
    CAAACGGAAAACTCACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCGTGGCCAACACTTGTCACTACTCTGACCTATGGTGTTCAATGCTTTTCCCGTTATCCGGATCACATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGGTTTGCCTTTTGAGTGGGAATTTAAATAAACGTGATGACCTTTTGATGGACAAGGCACCGGTTGTGAACAGTGATGAGACTGGATACCACAAGTTACGAAAAGGGCAATAGGCCTAGTGTACTTTGCCGTACTGAAAAAGTTCTCACGGTACGGGCTTCCAATACATGTCCTTGCBarIMscIBspEIBsrGIsfGFP360380400420440460480500520
    CACTATATCTTTCAAAGATGACGGGACCTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAGTTAAAGGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAACTCGAGTACAACTTTAACTCACACAATGTATACATCACGGCAGTGATATAGAAAGTTTCTACTGCCCTGGATGTTCTGCGCACGACTTCAGTTCAAACTTCCACTATGGGAACAATTAGCATAGCTCAATTTCCCATAACTAAAATTTCTTCTACCTTTGTAAGAACCTGTGTTTGAGCTCATGTTGAAATTGAGTGTGTTACATATGTAGTGCCGTAvaIBaeIMluITliIXhoIBsoBIPpuMIPspXIAflIIIPaeR7IBstZ17IEcoO109IsfGFP540560580600620640660680700
    GACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTCGCCACAACGTTGAAGATGGTTCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCGACACAATCTGTCCTTTCGAAAGATCCCAACGCTGTTTGTTTTCTTACCTTAGTTTCGATTGAAGTTTTAAGCGGTGTTGCAACTTCTACCAAGGCAAGTTGATCGTCTGGTAATAGTTGTTTTATGAGGTTAACCGCTACCGGGACAGGAAAATGGTCTGTTGGTAATGGACAGCTGTGTTAGACAGGAAAGCTTTCTAGGGTTGCAclIMfeISalIBstBIHincIIsfGFP720740760780800820840860
    AAAAGCGTGACCACATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTACAAATAAGGATCTGAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTTTTCGCACTGGTGTACCAGGAAGAACTCAAACATTGACGACGACCCTAATGTGTACCGTACCTACTCGAGATGTTTATTCCTAGACTTCGAACCCGGGCTTGTTTTTGAGTAGAGTCTTCTCCTAGACTTATCGCGGCAGCTGGTAGTAGTAGTAGTAGTAACTCAAATTTGCCApaIDrdIEarIPmeISacISalIBseYIHincIIPspOMIEco53kIHindIIItrrnbsfGFP8809009209409609801,0001,0201,040
    TCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGAGAGGTCGAACCGACAAAACCGCCTACTCTCTTCTAAAAGTCGGACTATGTCTAATTTAGTCTTGCGTCTTCGCCAGACTATTTTGTCTTAAACGGACCGCCGTCATCGCGCCACCAGGGTGGACTGGGGTACGGCTTGAGTCTTCACTTTGCGGCATCGCGGCTACCATCACACBsaIXmnItrrnb1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACTGGATCCTTACTCGAGTCTAGACTGCAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATCCCAGAGGGGTACGCTCTCATCCCTTGACGGTCCGTAGTTTATTTTGCTTTCCGAGTCAGCTTTCTGACCCGGAAAGCAAAATAGACAACAAACAGCCACTTGACCTAGGAATGAGCTCAGATCTGACGTCAACTAGCCCGTGCATTCTCCAAGGTTGAAAGTGGTATTACTTTAAvaIBmrIBsaIMslIPstISfcITliIXbaIXhoIBamHIBsaAIBsoBIPspXIPaeR7ItrrnbCmR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGTTCTAGTGATGGCCCGCATAAAAAACTCAATAGCTCTAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGTGACCTATATGGTGGCAACTATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATGGATATTGGTCTGGCAAGTCBpu10ICmR1,4201,4401,4601,4801,5001,5201,5401,560
    CTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGGACCTATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATTCGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCTTAAAGCATACCGTTACTTTCTGCCACTCGACCACTATACCCTATCACAAGTGGGAACAATGTGGCAAAAGGTACBsmIFauIBspEIBsrDIPvuIICmR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTCGTTTGACTTTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCTAAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCACAATGCCACTTTTGGACCGGATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTCAAAGTGGTCAclIPasIBsmBIEsp3IPflMICmR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATAAAACTAAATTTGCACCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTGGTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGTAGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAATTACTTAATGTTGTCATGACGCTABsmIMscINcoIScaISspIStyICmR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAGTGGCAGGGCGGGGCGTAATTTGATATCGAGCTCGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCCTCCGATCAACGTCTCATTTTCGCCAAAAGTTGCTCACCGTCCCGCCCCGCATTAAACTATAGCTCGAGCGAACCTGAGGACAACTATCTAGGTCATTACTGGAGTCTTGAGGTAGACCTAAACAAGTCTTGCGAGCCAACGGCGGCCCGCAAAAAATAACCACTCTTAGGTTCGGAGGCTAGTTGCAGAGTAAAAGCGGTTTTCAACFauISacIBsmBIEcoRVEsp3IPflMIEco53kICmR2,1202,1402,1602,1802,2002,2202,2402,260
    GCCCAGGGCTTCCCGGTATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCGGGTCCCGAAGGGCCATAGTTGTCCCTGTGGTCCTAAATAAATAAGACGCTTCACTAGAAGGCAGTGTCCATAAATAAGCCGCGTTTCACGCAGCCCACTACGACGGTTGAATGACTAAATCACATACTACCACAAAAACTCCACGAGGTCACCGAAGACAAAGATAGTCGACAPasIPvuII2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACGGGAGGACAAGTCGATGACTGCCCCACCACGCATTGCCGTTTTCGTGGCGGCCTGTAGTCGCGATCGCCTCACATATGACCGAATGATACAACCGTGACTACTCCCACAGTCACTTCACGAAGTACACCGTCCTCTTTTTTCCGACGTGGCCACGCAGTCGTCTTATACACTATGAfeIAgeIBmtIBsgINheIXmnIAlwNISgrAIBstZ17Ip15a2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AGGATATATTCCGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGATCCTATATAAGGCGAAGGAGCGAGTGACTGAGCGATGCGAGCCAGCAAGCTGACGCCGCTCGCCTTTACCGAATGCTTGCCCCGCCTCTAAAGGACCTTCTACGGTCCTTCTATGAATTGTCCCTTCACTCTCCCGGCGCCGTTTCGGCAAAAAGGTATCCGAGGCGGGGGGACTPfoIBsiEIBsrBISacIIp15a2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTGTTCGTAGTGCTTTAGACTGCGAGTTTAGTCACCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCGCCGAGGGAGCACGCGAGAGGACAAGGACGGAAAGCCAAATGGCCACAGTAAGGCGACAATACCGGCGCAAACAGAGTAAGGTGCGGACTGTGAAgeIBssSIBssSαIp15a2,8202,8402,8602,8802,9002,9202,9402,960
    CAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCGTCAAGGCCCATCCGTCAAGCGAGGTTCGACCTGACATACGTGCTTGGGGGGCAAGTCAGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCTTTCTGTACGTTTTCGTGGTGACCGTCGTCGGTGACCATTAACTAAATCTCCTCAATCAGAACTTCAGNspIAlwNIBseRIBsiEIp15a2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ATGCGCCGGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGATCTCAAGAATACGCGGCCAATTCCGATTTGACTTTCCTGTTCAAAACCACTGACGCGAGGAGGTTCGGTCAATGGAGCCAAGTTTCTCAACCATCGAGTCTCTTGGAAGCTTTTTGGCGGGACGTTCCGCCAAAAAAGCAAAAGTCTCGTTCTCTAATGCGCGTCTGGTTTTGCTAGAGTTCTTBseRIBstBIp15a3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GATCATCTTATTAATCAGATAAAATATTTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATATAAGTTGTAATTCTCATGTTTGACAGCTTATCATCGATAAGCTTCCGATGGCGCGCCGAGAGGCTTTACACTTTATGCTTCCGGCTCTAGTAGAATAATTAGTCTATTTTATAAAGATCTAAAGTCACGTTAAATAGAGAAGTTTACATCGTGGACTTCAGTCGGGGTATGCTATATTCAACATTAAGAGTACAAACTGTCGAATAGTAGCTATTCGAAGGCTACCGCGCGGCTCTCCGAAATGTGAAATACGAAGGCCGAAscIAseIClaIEarISspIXbaIBspDIBssHIIHindIIINmeAIIIp15a3,3403,3603,3803,4003,4203,4403,4603,4803,500
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    3500 bp2500275030003250250500750p15asfGFPScar

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    3500