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    Scallop RNA Extractions
    • Notes
    • Metadata
    • Relevant Items
    • RNA Extraction - Trizol and PureLink Kit
    Editing disabled on read-only entry.

     
    Scallop RNA Extractions

    Wednesday, 9/13/2017metadata_20170515.xlsx.xlsxSample QCWednesday, 10/4/2017Friday, 10/6/2017Tuesday, 10/10/2017Thursday, 10/12/2017Wednesday, 10/18/2017Thursday, 11/2/2017Scallop_Full_Transcriptome.xlsxMonday, 12/4/2017
    Wednesday, 9/13/2017
    RNA extractions for a full transcriptome of rock scallops (Crassodoma gigantea). I will be submitting 8 pools of samples to the UChicago Functional Genomics Core to sequence- 4 tissues (gonad, ctenidia, mantle, abductor muscle) and two treatments (low pH and ambient). Each pool will have up to 8 different individials- 2 from each of the 4 populations.
    metadata_20170515.xlsx.xlsx
    Wednesday, 10/4/2017
    PureLink Kit. Eluted in 125uL water that was run through the column 2x.
    ●
    39_gonad
    ●
    39_mantle
    ●
    370_mantle
    ●
    370_abductor
    ●
    312_gonad
    ●
    312_ctenidia
    ●
    242_ctenidia
    ●
    242_abductor
    ●
    310_gonad
    ●
    310_abductor
    ●
    310_mantle
    ●
    310_ctenidia
    ●
    361_gonad
    ●
    361_mantle
    ●
    361_ctenidia
    ●
    36_ctenidia
    Friday, 10/6/2017
    ●
    391_M, 391_G, 391_A, 391_C
    ●
    360_C, 360_A,360_G, 360_M
    ●
    241_A, 241_C, 241-G, 241_M
    Tuesday, 10/10/2017
    ●
    372_mantle, 372_abductor, 372_ctenidia, 390_G, 390_M, 390_C, 36_A, 36_M, 36_G, 305_A, 305_C, 305_G, 305_M, 53.A_A, 53.A_C, 53.A_G
    Thursday, 10/12/2017
    311_a, 311_g
    391_a,m,g,c
    234_a, m, g, c
    53.a_m
    Wednesday, 10/18/2017
    Extract after Bioanalyzer results
    360_A
    234_A
    245_A
    381_ctenidia
    390_c or 391_c
    Thursday, 11/2/2017
    Samples pooled following this Google Sheet and submitted to core for sequencing!
    Scallop_Full_Transcriptome.xlsx
    Monday, 12/4/2017
    RNA data in hand!

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