Welcome to Benchling!

You're looking at a Notebook entry. Here are some things you can do with it:

Track every step of your experiment

  1. Add new dates for every day your experiment runs.
  2. Capture protocols, observations, and tasks.
  3. Automatically keep a detailed history of your work.
  4. Auto-populate your calendar with Notebook entries.

Insert tables with calculations

Link sequences directly in your entry

Attach images and results

Share data with your collaborators

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
0 of 0
Advanced Settings
    24 QuantSeq Libraries
    • Notes
    • Metadata
    • Relevant Items
    • QuantSeq_Illumina_11_27_17.pdf
    • QuantSeq 3' mRNA Kit
    Editing disabled on read-only entry.

     
    24 QuantSeq Libraries

    Wednesday, 12/13/2017Oyster samplesTable1Friday, 12/15/2017Table2Wednesday, 12/20/2017Pfister__Total_RNA_Nano_2017-12-21_1of2_AC.pdfPfister__Total_RNA_Nano_2017-12-21_2of2_AC.pdfLibrary_24AWednesday, 12/27/2017Library_24BFriday, 12/29/2017Saturday, 12/30/2017Friday, 1/5/2018
    Wednesday, 12/13/2017
    Going to run 24 QuantSeq libraries for each species on 2 SE 50bp HiSeq lanes.
    Friday, 12/15/2017
    Re-extract:
    ●
    234_G
    ●
    391_G
    ●
    390_G
    ●
    241_G
    ●
    241_C
    ●
    372_M
    ●
    372_A
    Did Qubit
    Concentrate dilute samples using Zymo RNA Clean & Concentrator -5 kit.
    Wednesday, 12/20/2017
    Submit some samples to Core Bioanalyzer. Will do only 24 libraries today
    ●
    372_M (new)
    ●
    241_G (new)
    ●
    390_G (new)
    ●
    391_G (new)
    ●
    234_G (new)
    ●
    BC1B_16A
    ●
    OR1A_1A
    Pfister__Total_RNA_Nano_2017-12-21_1of2_AC.pdf
    Pfister__Total_RNA_Nano_2017-12-21_2of2_AC.pdf
    1st set of 24 libraries. Tried to get 500ng of RNA for each sample, but some samples had between 213-473
    Did not skip step 2 correctly for FFPE samples
    Went to Step 11.
    Wednesday, 12/27/2017
    Do 2nd library of 24 up to Step 11.
    13 extra reactions through Step 6
    Friday, 12/29/2017
    PCR
    switched CA1A_6A and 36_WAS_G in plate position after PCR (D2 and D1)
    Left 391CA_M (cycle 18) on the PCR machine overnight without starting it. Will go ahead with purification but might not sequence.
    Saturday, 12/30/2017
    Took Cycle 20 and 22 off the cycler (left overnight at 0degC). PCR purification step.
    switched A2 (CAB1_14A) and Test_8(A7) in plate after PCR, but then fixed.
    switches A3 and C3-> NF_28_MA did not get the proper FFPE treatment with reduced PB
    Check quality of 24 samples on Bioanalyzer
    Bioanalyzer run A: 1-9 and 2 test wells
    Friday, 1/5/2018
    Pooled Library A and turned in to sequencing center!
    I should pool myself- lower variance.

    Welcome to Benchling!

    Sign in to view data.

    Split Workspace