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❗️❗️Working in R'n'R lab
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❗️❗️Working in R'n'R lab

Tuesday, 9/15/2020
Tuesday, 9/15/2020
We try to work in a collaborative environment that allows us to learn science and produce knowledge.
All lab members help each other in a healthy and productive lab. Publication is a (required) way, not a goal.
This phylosophy is nowadays more common than ever. For example, see: http://www.avasthilab.org/lab-policies-and-tips/
Here you are some tips that I always need to remind students and myself every now and then:
1.
Ask! Ask always, no matter how many times, we are here to help. But, please, take notes to avoid unnecessary repetitions.
2.
You don't work for your supervisor, you work with the supervisor on your project. And you can also rely on your lab mates and collaborators.
3.
Think in the experiments one by one, depending on the objectives we want to achieve, the bibliography, and the previous results.
4.
Don't wait until you have to write to read. Reading papers is the only why to be progress in your research.
5.
Prepare protocol before doing the experiments, following the scientific method:
a.
Objectives
b.
Material and reagents
c.
Steps to follow, as detailed as possible the first few times. Controls!
d.
Results obtained and problems / consequences for the next time
e.
Conclusions
6.
Gather ALL the necessary material before doing the experiment and plan the time it may take based on the time available.
7.
If some reagent is about to finish: order or prepare, do not leave tubes / boxes empty for the next user.
8.
The laboratory notebook should be generally updated and available online for the whole lab. This is very important for the preparation of the final report or thesis (TFG, TFM, PhD ...) and for possible publications, but also for our daily work after several hundreds of experiments. It is also essential for future lab members that may continue your project.
a.
We prefer Benchling, but other platforms are also fine. See: https://www.nature.com/articles/d41586-018-05895-3
b.
Update it (at least) every week, specially if you are going to be out of the lab.
9.
Yes, in bioinformatics we also use a laboratory notebook and it is indeed possible to keep it updated. See: https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1004385 and https://blog.addgene.org/how-to-keep-a-lab-notebook-for-bioinformatic-analyses
10.
Any lab mate is willing to help and share protocols, reagents or material. That must be reciprocal.
11.
Respect safety regulations: gloves, gown, collected hair, ...
12.
Use the pipettes carefully: avoid remaining volume at the tip (inside or outside if it is dense), avoid aerosols (especially in radioactive samples or with a high bacteria / phage titer) ... Ask for calibration if they don't work properly.
13.
Never send your TFG, TFM, or meeting abstract/poster to your supervisor the very last day of the submission. The final mark and quality are proportional to the times that other people can review a manuscript and that needs time.
14.
We can make as many rehearsals as you need, but that also needs to be planned in advance.

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