Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV_hSyn FLEX-HA-hM3D(Gq)-P2A-GCaMP6m
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagactgcagagggcggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcacacagatctgacgtctcccgEagIMluINotIXbaIAflIIIPspOMIL-ITRHuman S...omoter20406080100120140160
    cctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccggacgcatactcacgttcacccaaaatcctggtcctactccgccccacccccacggatggactgctggctggggctgggtgacctgttcgtgggttgggggtaaggggtttaacgcgtaggggatagtctctccccctcccctttgtcctacgccgctccgcgcacgcgtgacggApaIFspAIHuman Synapsin promoter180200220240260280300320340
    agcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgctcgaagtcgtggcgcctgtcacggaagcgggggcggaccgccgcgcgcggtggcggcggagtcgtgacttccgcgcgactgcagtgagcggccagggggcgtttgaggggaagggccggtggaaccagcgcaggcgcggcggcggccgggtcggcctggcgtggtgcgctccgcgBbvCIRsrIISacIIHuman Synapsin promoter360380400420440460480500520
    gagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgagaaggtaccggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagctctatccccccgtgcccgcgctggtagacgcgacgccgcggccgctgagtcgcgacggagtcagacgccacccgtcgcctcctcagcacagcacggactctcgcgtcagctcttccatggcctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcAccIAfeIKasIMreINarISalISfoIXbaIBamHIBspEIPluTISgrAIHuman Synapsin promoterlox2722540560580600620640660680700
    ttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgcctcacttcgctgtcatcatttgtacaaactcttcgtagtttacctgaccatccccatcgatgtctgcttccctgatcattaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcggagtgaagcgacagtagtaaacatgtttgagaagcatcaaatggactggtaggggtagctacagacgaagggactagtaaAscIBarIBclIBsrGI*K1074AT1072MM1070QV1068FE1066EY1064NV1062QG1060DG1058DI1056DA1054ER1052IM1050797-4021GCaMP6mloxP720740760780800820840860
    tcatcaacctcttcatctgttaacttctctccaaggtttgtcatcacgtggcgaagctctgctgcactgatgtagccattgccatccttatcaaacacaccgaacgcttctctaatttcttcttccgtgtccctgtagctcccttttcttgccatcattgtcaggaactcagggaagtagttggagaagtagacaattgaagagaggttccaaacagtagtgcaccgcttcgagacgacgtgactacatcggtaacggtaggaatagtttgtgtggcttgcgaagagattaaagaagaaggcacagggacatcgagggaaaagaacggtagtaacagtccttgagtccctHpaIPmlIED1048VE1046ED1044TL1042KE1040GL1038NT1036MV1034HR1032LE1030AA1028SI1026YG1024NG1022DK1020DF1018VG1016FA1014ER1012IE1010EE1008TD1006RY1004SG1002KR1000AM998MT996LF994EP992F797-4021GCaMP6m8809009209409609801,0001,0201,040
    agtcgattgtgccgtcaccgtcggcatctacttcattgatcatgtcctgcagctctgcttctgtggggttctgccccagagaccgcatcaccgtccccagctccttggttgttattgtcccatccccgtccttgtcaaatagggagaaagcctctttaaattctgcgatctgctctcagctaacacggcagtggcagccgtagatgaagtaactagtacaggacgtcgagacgaagacaccccaagacggggtctctggcgtagtggcaggggtcgaggaaccaacaataacagggtaggggcaggaacagtttatccctctttcggagaaatttaagacgctagacgagBclIBstXID990IT988GD986GD984AD982VE980NI978MD976QL974EA972ET970PN968QG966LS964RM962VT960GL958EK956TT954IT952GD950GD948KD946FL944SF942AE940KF938EA936IQ934E797-4021GCaMP6m1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttcagtcagttggtccggcaggttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggaagtcagtcaaccaggccgtccaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagctacgggaagtcgagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggaccSapIBspQIE932TL930QD928PL926NY924EL922KH920GL918IN916GD914EK912FD910IG908KL906EI904RN902VL900TD898GE896FK894VE892AR890TK888YN886GD884DK882FF880IT878RE876Q797-4021GCaMP6m1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgctacatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgccgacttcgtgacgtgcggcatccagtcccaccagtgctcccacccggtcccgtgcccgtcgaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgI874YG872EP870MA868SK866FF864DH862QK860MH858DP856YR854SF852CQ850VG848YT846LT844TV842LT840PW838PV836PL834KG832TT830CI828FK826LT824LK822GY820TA818DG816797-4021GCaMP6m1,4201,4401,4601,4801,5001,5201,5401,560
    cctcaccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatgctccctccggtaccgcccttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactcggagtgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtggtggggccacttgtcgaggagcgggaacgagtggtacgagggaggccatggcgggaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgagBsrGIEG814EG812SV810SF808KH806GN804VD802GD800LE798VL796IP794VV792GT790FL788EE786GK784SV782MS780GG778TG776GK774YL772ED770MG768LT766IG764AA762TV760FE758797-4021GCaMP6m1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cagcaggaccatgtgatcgcgcttctcgttggggtctttcgaaagtttggactgcacgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtaggcgagctgcacgccgccgtcctcgatgttgtggcggatcttgaagttcgtcgtcctggtacactagcgcgaagagcaaccccagaaagctttcaaacctgacgtgcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccatccgctcgacgtgcggcggcaggagctacaacaccgcctagaacttcaagBstBILL756VM754HD752RK750EN748PD746KS744LK742SQ740VS738LY736HN734DP732LL730VP728GD726GI724PT722NQ720QY718HY716AL714QV712GG710DE708IN706HR704IK702FN700797-4021GCaMP6m1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gccttgatgccgttcttctgcttgtcggccttgatatagacgttctcgagtgagctcagccgacctatagctctgactgcgtgacctgtcttattccacttacgacgtgatgagtcgaccatggtggcgagatccttatcgtcatcgtcgtacagatcccgacccatttgctgtccggaactacggcaagaagacgaacagccggaactatatctgcaagagctcactcgagtcggctggatatcgagactgacgcactggacagaataaggtgaatgctgcactactcagctggtaccaccgctctaggaatagcagtagcagcatgtctagggctgggtaaacgacagAccIBlpINcoISacISalIBmgBIBsaBIEco53kIAK698IG696NK694QK692DA690KI688YV686NE684LS682SL680RG678IA676RV674AH672GT670KN668WK666RR664SS662DV660MT658AL656DK654DD652DD650YL648DR646GM644QQ642G797-4021GCaMP6m1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caccagtcatgctggccataccatgatgatgatgatgatgagaacccatgggtccagggttctcctccacgtctccagcctgcttcagcaggctgaagttagtagctccgcttccaccggtcaaggcctgctcgggtgcgcgcttgtgaaaaatgaccgactgtctctgctggtagtggtcagtacgaccggtatggtactactactactactactcttgggtacccaggtcccaagaggaggtgcagaggtcggacgaagtcgtccgacttcaatcatcgaggcgaaggtggccagttccggacgagcccacgcgcgaacactttttactggctgacagagacgaccatAgeIBarINcoIStuIBmgBIG640TM638SA636MG634HH632HH630HH628SG626MP624GP622NE620EV618DG616AQ614KL612LS610FN608TA606GS604GG602TL600AQ598EP596AR594KH592FI590VS588QR586QQ584Y797-4021P2AhM3D(Gq)GCaMP6m2,1202,1402,1602,1802,2002,2202,2402,260
    ctgctgcttgcgcctcttttttttgtcacactggcacagcagcagcatcttgaaagtggttctgaatgttttgttgcacagagcatagcacacggggttcacggtgctgttgatgtagcacagccagtagcccagattccaaaaggttttgggtatgcagctgtcacaaaaggtggacgacgaacgcggagaaaaaaaacagtgtgaccgtgtcgtcgtcgtagaactttcaccaagacttacaaaacaacgtgtctcgtatcgtgtgccccaagtgccacgacaactacatcgtgtcggtcatcgggtctaaggttttccaaaacccatacgtcgacagtgttttccacQ582QK580RR578KK576KD574CQ572CL570LL568MK566FT564TR562FT560KN558CL556AY554CV552PN550VT548SN546IY544CL542WY540GL538NW536FT534KP532IC530SD528CF526T797-4021hM3D(Gq)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttcaccagaaccatgatgttgtatggggtccaagtgatgatgaaggcaagcaagatcgcactgagggtctgggccgctttcttctccttgaccagggacatccttttccgcttagtgatctgacttctggtcttcagagcaaacctcttggccagagtggcttccttgaaggacaaagtggtcttggtactacaacataccccaggttcactactacttccgttcgttctagcgtgactcccagacccggcgaaagaagaggaactggtccctgtaggaaaaggcgaatcactagactgaagaccagaagtctcgtttggagaaccggtctcaccgaaggaacttcctgtEcoNIN524VL522VM520IN518YP516TW514TI512IF510AL508LI506AS504LT502QA500AK498KE496KV494LS492MR490KR488KT486IQ484SR482TK480LA478FR476KA474LT472AE470KF468SL466797-4021hM3D(Gq)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gaggtagagtggccgtgctcttacccactgaggagttgacgtcagaagtcttagctgtgtccacggctgactctagctggatgggaagcttggagaagctttttggaaaactgcctccatcgtccacgctcttctgggcctgcagcttgtcggctttcctctccaagtccaccatctccatctcaccggcacgagaatgggtgactcctcaactgcagtcttcagaatcgacacaggtgccgactgagatcgacctacccttcgaacctcttcgaaaaaccttttgacggaggtagcaggtgcgagaagacccggacgtcgaacagccgaaaggagaggttcaggtggtaSapIBspQIPL464TA462TS460KG458VS456SN454VD452ST450KA448TD446VA444SE442LQ440IP438LK436SF434SK432PF430SG428GD426DV424SK422QA420QL418KD416AK414RE412LD410VM408797-4021hM3D(Gq)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccccagctcctcctcaggcacctgcaggttgtccgatgagggtaacttggtggagttgaggatggtgctgtgacccggaagcttgagcacgatggagtagatggctctcgtctcggagccaatgtcctcctcgtcggaggaggcggagttctccagggaggcagcagcatcattgggggtcgaggaggagtccgtggacgtccaacaggctactcccattgaaccacctcaactcctaccacgacactgggccttcgaactcgtgctacctcatctaccgagagcagagcctcggttacaggaggagcagcctcctccgcctcaagaggtccctccgtcgtcgtagtaacPaqCIBsu36IGL406EE404EP402VQ400LN398DS396SP394LK392TS390NL388IT386SH384GP382LK380LV378IS376YI374AR372TE370SG368ID366EE364DS362SA360SN358EL356SA354AA352DN350797-4021hM3D(Gq)2,8202,8402,8602,8802,9002,9202,9402,960
    ttgttccaactgtcactgctgctgtggtcttggtccatctgctcggagctgggtttccagctcttggttgtgaaccagaagtggcagcggccatacttcctcctgttggagcgtttcatgctttgctgttgaagttcgtaactgctgcagcttcgagaactgcccgtggggtggaaacaaggttgacagtgacgacgacaccagaaccaggtagacgagcctcgacccaaaggtcgagaaccaacacttggtcttcaccgtcgccggtatgaaggaggacaacctcgcaaagtacgaaacgacaacttcaagcattgacgacgtcgaagctcttgacgggcaccccacctPflMINN348WS346DS344SS342HD340QD338MQ336ES334SP332KW330SK328TT326FW324FH322CR320GY318KR316RN314SR312KM310SQ308QQ306LE304YS302SC300SR298SS296GT294PH292V797-4021hM3D(Gq)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    caaagttttctgtctctgcctctgtcccagaggcttgcaggccagcaagctctttggtacgcttttcagtttccttatagatcctccagtataaaatagtcataatggtgacaggcatataaaaaccagcgatggctgtgccaaaagtaatggtgggctcactgaggaactgaatgtttcaaaagacagagacggagacagggtctccgaacgtccggtcgttcgagaaaccatgcgaaaagtcaaaggaatatctaggaggtcatattttatcagtattaccactgtccgtatatttttggtcgctaccgacacggttttcattaccacccgagtgactccttgacttaF290NE288TE286AE284TG282SA280QL278GA276LE274KT272RK270ET268EK266YI264RW262YL260IT258MI256TV254PM252YF250GA248IA246TG244FT242IT240PE238SL236FQ234I797-4021hM3D(Gq)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gaagcactctcccggaggcacagttctctttccaacaaagtattgccagaacaagatggcaggagcccaaaggacaaaggagatgacccaagccagaccgatcatcacaccggctctctttgttgttcgtttggctcggtacgtgagcggcctcgtgatggaaaagtatctgtcacttcgtgagagggcctccgtgtcaagagaaaggttgtttcataacggtcttgttctaccgtcctcgggtttcctgtttcctctactgggttcggtctggctagtagtgtggccgagagaaacaacaagcaaaccgagccatgcactcgccggagcactaccttttcatagacagtF232CE230GP228PV226TR224KG222VF220YQ218WF216LI214AP212AW210LV208FS206IV204WA202LG200IM198VG196AR194KT192TR190KA188RY186TL184PR182TI180SF178YR176D797-4021hM3D(Gq)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aagctgatgaccagaagattcataacagaggcattgctggctacgcagtcaatggcaagccagaggtcacaggccaagttccctaaggcccatcgattcatgatgatgtaggtcgtaaacagattcattgaaatgaccccgataatcagatcggcacaggccaggcttaagaggattcgactactggtcttctaagtattgtctccgtaacgaccgatgcgtcagttaccgttcggtctccagtgtccggttcaagggattccgggtagctaagtactactacatccagcatttgtctaagtaactttactggggctattagtctagccgtgtccggtccgaattctcctAflIIBsu36IF174SI172VL170LN168MV166SA164NS162AV160CD158IA156LW154LD152CA150LN148GL146AW144RN142MI140IY138TT136FL134NM132SI130VG128II126LD124AC122AL120SL118LF116797-4021hM3D(Gq)3,5203,5403,5603,5803,6003,6203,6403,660
    agtagttgttgaccgtcttcagctgcttgttgaccttaaatgacacaattaccaggatgttgccgatgatggtcaccaaggccaggatgcccgttaagaaagcgatgaagaccacttgccagacggtatgacctcccagagggtcatcggtggtaccgtctggagaggagaaatttcatcaacaactggcagaagtcgacgaacaactggaatttactgtgttaatggtcctacaacggctactaccagtggttccggtcctacgggcaattctttcgctacttctggtgaacggtctgccatactggagggtctcccagtagccaccatggcagacctctcctctttaaEcoNIBstEIIYN114NV112TK110LQ108KN106VK104FS102VI100VL98IN96GI94IT92VL90AL88IG86TL84FA82IF80VV78QW76VT74HG72GL70PD68DT66TG64DP62SS60FN58797-4021hM3D(Gq)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gccagctgctcgagaaacattgtagctgccgaaatgagtgacggttcccgggggcagccctgcatcggaggggctgtgtatccaggaggagctgatgtttggaaacaaaggcgaggttgtactgttattgtgcaaggtcatagcgtaatctggaacatcgtatgggtacatggtgcggtcgacgagctctttgtaacatcgacggctttactcactgccaagggcccccgtcgggacgtagcctccccgacacataggtcctcctcgactacaaacctttgtttccgctccaacatgacaataacacgttccagtatcgcattagaccttgtagcatacccatgtaccacGA56AR54SV52NY50SG48FH46TV44TG42PP40LG38AD36SP34SH32IW30SS28SI26NP24FL22PS20TT18SN16NH14LT12MA10YD8PV6DY4PY2M797-4021hM3D(Gq)HA tag3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcggatccgagctcggtaccaagcttaagtttaaacgctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcgcctaggctcgagccatggttcgaattcaaatttgcgatcgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcBmtINheIPmeISacIAflIIBamHIEcoRIEcoRVEco53kIlox2722loxP4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagWPRE4,2204,2404,2604,2804,3004,3204,3404,360
    tttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacPflMIWPRE4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggcSacIIWPRE4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagcgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcAfeIBmrIBglIIWPREhGH polyA4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgaCspCIhGH polyA4,9204,9404,9604,9805,0005,0205,0405,060
    gcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttcgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaBlpINsiISphIBbvCIhGH polyA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgccccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcggEagINotIPmlIBstXIRsrIIBstEIIR-ITRhGH polyA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtgctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaKasINarISfoIPluTIR-ITRf1 Origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaf1 Origin5,6205,6405,6605,6805,7005,7205,7405,760
    ttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggtaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaPsiIDraIIIf1 Origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    taaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatcattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtag5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcgcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcg6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcaccccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtggAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    cagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgagtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggagcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctPvuIScaIAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcAmpR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagtgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaAseIBmrIAmpR7,0207,0407,0607,0807,1007,1207,1407,160
    tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaaAmpR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctacctattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatgpUC ori7,3607,3807,4007,4207,4407,4607,4807,5007,520
    caactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttacgttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatgAlwNIpUC ori7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggcpUC ori7,7207,7407,7607,7807,8007,8207,8407,860
    gtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggtcattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccapUC ori7,8807,9007,9207,9407,9607,9808,0008,0208,040
    tcctggccttttgctggccttttgctcacatgtaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori8,0608,080
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8083 bp6000650070007500500100015002000GCaMP6m797-4021

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    8083