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pLenti6.3/V5-HA-hM4D(Gi)-mCherry
  • Sequence Map
    Editing disabled on read-only sequence.
    aatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactttacatcagaatacgttatgagaacatcagaacgttgtaccattgctactcaatcgttgtacggaatgttcctctctttttcgtggcacgtacggctaaccaccttcattccaccatgctagcacggaataatccttccgttgtctgcccagactgtacctaacctgcttggtgaBaeIRSV promoterpRSV/5'LTR20406080100120140160
    gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtcttaacggcgtaacgtctctataacataaattcacggatcgagctatgtatttgcccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacapRSV/5'LTRHIV-1 5' LTRRSV promoter180200220240260280300320340
    gtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtcactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgcgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcapRSV/5'LTRHIV-1 5' LTR360380400420440460480500520
    acgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgtgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttcttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcNruIHIV-1 psi packaging signal540560580600620640660680700
    cagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgggagataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgtt720740760780800820840860
    gatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcactatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtMfeINotISapIBspQIEcoNI8809009209409609801,0001,0201,040
    gagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatatcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacBbvCIRRE1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaaaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgacgacacggaaccttacgatcaacctcattatttagagaccttgtctaaccttBsmIBsaBIRRE1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatttacccgttcaaacaccttaaccaaattgtattgtttaaccgacaccatatat1,4201,4401,4601,4801,5001,5201,5401,560
    aaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagtttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggctcccctgggctgtccgggcttccttatcttcttcttccacctctctctcKflIPpuMI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acagagacagatccattcgattagtgaacggatctcgacggtatcggttaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatgtctctgtctaggtaagctaatcacttgcctagagctgccatagccaattgaaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtttgatttcttaatgtttttgtttaatgtttttaagttttaaaatagctClaIHpaIBspDIcPPT1,7601,7801,8001,8201,8401,8601,8801,9001,920
    taagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagattcgaaccctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcPcmv1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatggatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttaccNdeISnaBIPcmv2,1202,1402,1602,1802,2002,2202,2402,260
    gcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgaPcmv2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgactctagaggatccactagtggatcctctagagtcgacgccaccatggccaacttcacacctgtcaatggcagctcgggcaatcagtccgtgcgcctggtcacgtcagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggctgagatctcctaggtgatcacctaggagatctcagctgcggtggtaccggttgaagtgtggacagttaccgtcgagcccgttagtcaggcacgcggaccagtgcagtSalISpeIBmgBIBsmBIEsp3IMA2NF4TP6VN8GS10SG12NQ14SV16RL18VT20S2563-4731PcmvhM4D2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tcatcccacaatcgctatgagacggtggaaatggtcttcattgccacagtgacaggctccctgagcctggtgactgtcgtgggcaacatcctggtgatgctgtccatcaaggtcaacaggcagctgcagacagtcaacaactacttcctcttcagcctggcgtgtgctgatctcaagtagggtgttagcgatactctgccacctttaccagaagtaacggtgtcactgtccgagggactcggaccactgacagcacccgttgtaggaccactacgacaggtagttccagttgtccgtcgacgtctgtcagttgttgatgaaggagaagtcggaccgcacacgactagagtBsmBIEsp3IS22SH24NR26YE28TV30EM32VF34IA36TV38TG40SL42SL44VT46VV48GN50IL52VM54LS56IK58VN60RQ62LQ64TV66NN68YF70LF72SL74AC76AD78L2563-4731hM4D2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcataggcgccttctccatgaacctctacaccgtgtacatcatcaagggctactggcccctgggcgccgtggtctgcgacctgtggctggccctggactgcgtggtgagcaacgcctccgtcatgaaccttctcatcatcagctttgaccgctacttctgcgtcaccaagcctctagtatccgcggaagaggtacttggagatgtggcacatgtagtagttcccgatgaccggggacccgcggcaccagacgctggacaccgaccgggacctgacgcaccactcgttgcggaggcagtacttggaagagtagtagtcgaaactggcgatgaagacgcagtggttcggagaPasII80IG82AF84SM86NL88YT90VY92II94KG96YW98PL100GA102VV104CD106LW108LA110LD112CV114VS116NA118SV120MN122LL124II126SF128DR130YF132CV134TK136PL1382563-4731hM4D2,8202,8402,8602,8802,9002,9202,9402,960
    cacctaccctgcccggcgcaccaccaagatggcaggcctcatgattgctgctgcctgggtactgtccttcgtgctctgggcgcctgccatcttgttctggcagtttgtggtgggtaagcggacggtgcccgacaaccagtgcttcatccagttcctgtccaacccagcagtgaccgtggatgggacgggccgcgtggtggttctaccgtccggagtactaacgacgacggacccatgacaggaagcacgagacccgcggacggtagaacaagaccgtcaaacaccacccattcgcctgccacgggctgttggtcacgaagtaggtcaaggacaggttgggtcgtcactggTY140PA142RR144TT146KM148AG150LM152IA154AA156WV158LS160FV162LW164AP166AI168LF170WQ172FV174VG176KR178TV180PD182NQ184CF186IQ188FL190SN192PA194VT1962563-4731hM4D2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tttggcacagccattgctggcttctacctgcctgtggtcatcatgacggtgctgtacatccacatctccctggccagtcgcagccgagtccacaagcaccggcccgagggcccgaaggagaagaaagccaagacgctggccttcctcaagagcccactaatgaagcagagcgtcaaaaccgtgtcggtaacgaccgaagatggacggacaccagtagtactgccacgacatgtaggtgtagagggaccggtcagcgtcggctcaggtgttcgtggccgggctcccgggcttcctcttctttcggttctgcgaccggaaggagttctcgggtgattacttcgtctcgcagtBfuAIBspMIFG198TA200IA202GF204YL206PV208VI210MT212VL214YI216HI218SL220AS222RS224RV226HK228HR230PE232GP234KE236KK238AK240TL242AF244LK246SP248LM250KQ252SV2542563-4731hM4D3,1603,1803,2003,2203,2403,2603,2803,3003,320
    agaagcccccgcccggggaggccgcccgggaggagctgcgcaatggcaagctggaggaggcccccccgccagcgctgccaccgccaccgcgccccgtggctgataaggacacttccaatgagtccagctcaggcagtgccacccagaacaccaaggaacgcccagccacagagcttcttcgggggcgggcccctccggcgggccctcctcgacgcgttaccgttcgacctcctccgggggggcggtcgcgacggtggcggtggcgcggggcaccgactattcctgtgaaggttactcaggtcgagtccgtcacggtgggtcttgtggttccttgcgggtcggtgtctcgaAfeIKK256PP258PG260EA262AR264EE266LR268NG270KL272EE274AP276PP278AL280PP282PP284RP286VA288DK290DT292SN294ES296SS298GS300AT302QN304TK306ER308PA310TE312L2563-4731hM4D3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtccaccacagaggccaccacgcccgccatgcccgcccctcccctgcagccgcgggccctcaacccagcctccagatggtccaagatccagattgtgacgaagcagacaggcaatgagtgtgtgacagccattgagattgtgcctgccacgccggctggcatgcgccctgcggcccaggtggtgtctccggtggtgcgggcggtacgggcggggaggggacgtcggcgcccgggagttgggtcggaggtctaccaggttctaggtctaacactgcttcgtctgtccgttactcacacactgtcggtaactctaacacggacggtgcggccgaccgtacgcgggacgccggXcmIS314TT316EA318TT320PA322MP324AP326PL328QP330RA332LN334PA336SR338WS340KI342QI344VT346KQ348TG350NE352CV354TA356IE358IV360PA362TP364AG366MR368PA370A2563-4731hM4D3,5203,5403,5603,5803,6003,6203,6403,660
    aacgtggcccgcaagttcgccagcatcgctcgcaaccaggtgcgcaagaagcggcagatggcggcccgggagcgcaaagtgacacgaacgatctttgccattctgctggccttcatcctcacctggacgccctacaacgtcatggtcctggtgaacaccttctgccagagctgcattgcaccgggcgttcaagcggtcgtagcgagcgttggtccacgcgttcttcgccgtctaccgccgggccctcgcgtttcactgtgcttgctagaaacggtaagacgaccggaagtaggagtggacctgcgggatgttgcagtaccaggaccacttgtggaagacggtctcgacgtSfiISexAIN372VA374RK376FA378SI380AR382NQ384VR386KK388RQ390MA392AR394ER396KV398TR400TI402FA404IL406LA408FI410LT412WT414PY416NV418MV420LV422NT424FC426QS428C2563-4731hM4D3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tccctgacacggtgtggtccattggctactggctctgctacgtcaacagcaccatcaaccctgcctgctatgctctgtgcaacgccacctttaaaaagaccttccggcacctgctgctgtgccagtatcggaacatcggcactgccaggcgggatccaccggtcgccaccatggtagggactgtgccacaccaggtaaccgatgaccgagacgatgcagttgtcgtggtagttgggacggacgatacgagacacgttgcggtggaaatttttctggaaggccgtggacgacgacacggtcatagccttgtagccgtgacggtccgccctaggtggccagcggtggtaccaBfuAIBspMIPaqCIDraIIII430PD432TV434WS436IG438YW440LC442YV444NS446TI448NP450AC452YA454LC456NA458TF460KK462TF464RH466LL468LC470QY472RN474IG476TA478RR480DP482PV484AT486MV4882563-4731hM4DmCherry3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccctcgttcccgctcctcctattgtaccggtagtagttcctcaagtacgcgaagttccacgtgtacctcccgaggcacttgccggtgctcaagctctagctcccgctcccgctcccggcggggatgctcccgtgggtctggcggttcgacttccactggttcccaccgggggacgggBstEIISK490GE492ED494NM496AI498IK500EF502MR504FK506VH508ME510GS512VN514GH516EF518EI520EG522EG524EG526RP528YE530GT532QT534AK536LK538VT540KG542GP544LP5462563-4731mCherry4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaagcggaccctgtaggacaggggagtcaagtacatgccgaggttccggatgcacttcgtggggcggctgtaggggctgatgaacttcgacaggaaggggctcccgaagttcaccctcgcgcactacttgaagctcctgccgccgcaccactggcactgggtcctgaggagggacgAhdIBstEIIFA548WD550IL552SP554QF556MY558GS560KA562YV564KH566PA568DI570PD572YL574KL576SF578PE580GF582KW584ER586VM588NF590ED592GG594VV596TV598TQ600DS602SL6042563-4731mCherry4,2204,2404,2604,2804,3004,3204,3404,360
    aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccatcctgccgctcaagtagatgttccacttcgacgcgccgtggttgaaggggaggctgccggggcattacgtcttcttctggtacccgaccctccggaggaggctcgcctacatggggctcctgccgcgggacttcccgctctagttcgtctccgacttcgacttcctgccgccggtAloISbfIPflMIQD606GE608FI610YK612VK614LR616GT618NF620PS622DG624PV626MQ628KK630TM632GW634EA636SS638ER640MY642PE644DG646AL648KG650EI652KQ654RL656KL658KD660GG662H2563-4731mCherry4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacgatgctgcgactccagttctggtggatgttccggttcttcgggcacgtcgacgggccgcggatgttgcagttgtagttcaacctgtagtggagggtgttgctcctgatgtggtagcaccttgtcatgcttgcgcggctcccggcggtgaggtggccgccgtacctgctcgacatgXcmIBbvCISgrAIY664DA666EV668KT670TY672KA674KK676PV678QL680PG682AY684NV686NI688KL690DI692TS694HN696ED698YT700IV702EQ704YE706RA708EG710RH712ST714GG716MD718EL720Y2563-4731mCherry4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aagtaagaattcgatatcaagcttatcgactagtggatccgagctcggtaccaagcttaagtttaaaccgctgatcagcctcgactgtgccttctaaagggccaattctgcagatatccagcacagtggcggccgctcgagtctagagggcccgcggttcgaaggtaagcctatcttcattcttaagctatagttcgaatagctgatcacctaggctcgagccatggttcgaattcaaatttggcgactagtcggagctgacacggaagatttcccggttaagacgtctataggtcgtgtcaccgccggcgagctcagatctcccgggcgccaagcttccattcggatagBclINotIPmeISpeITliIXhoIBstBIEcoNIEcoRIEcoRVEcoRVPspXIPaeR7IK722*V5 ...pe4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cctaaccctctcctcggtctcgattctacgcgtaccggttagtaatgatcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggattgggagaggagccagagctaagatgcgcatggccaatcattactagctgttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataMluIWPREV5 epitope4,9204,9404,9604,9805,0005,0205,0405,060
    ggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctcccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagPfoIPflMIWPRE5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaaWPRE5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggcgatggtaccggtgtggaaagtccccaggctccccagcaggcagaagtatgcaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcggaccgctaccatggccacacctttcaggggtccgaggggtcgtccgtcttcatacgttBaeIWPRESV40 promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgNsiINsiISexAISV40 promoter5,6205,6405,6605,6805,7005,7205,7405,760
    taattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacaactgttaattagtagccgtatcatatagBclIPmlIAvrIISV40 promoterEM75,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggcatagtataatacgacaaggtgaggaactaaaccatggccaagcctttgtctcaagaagaatccaccctcattgaaagagcaacggctacaatcaacagcatccccatctctgaagactacagcgtcgccagcgcagctctctctagcgacggccgcatcttcactggtgtcaccgtatcatattatgctgttccactccttgatttggtaccggttcggaaacagagttcttcttaggtgggagtaactttctcgttgccgatgttagttgtcgtaggggtagagacttctgatgtcgcagcggtcgcgtcgagagagatcgctgccggcgtagaagtgaccacagtBlasticidinEM75,9605,9806,0006,0206,0406,0606,0806,1006,120
    atgtatatcattttactgggggaccttgtgcagaactcgtggtgctgggcactgctgctgctgcggcagctggcaacctgacttgtatcgtcgcgatcggaaatgagaacaggggcatcttgagcccctgcggacggtgccgacaggtgcttctcgatctgcatcctgggatcaatacatatagtaaaatgaccccctggaacacgtcttgagcaccacgacccgtgacgacgacgacgccgtcgaccgttggactgaacatagcagcgctagcctttactcttgtccccgtagaactcggggacgcctgccacggctgtccacgaagagctagacgtaggaccctagttNruIBsaBIPpuMIBlasticidin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    agccatagtgaaggacagtgatggacagccgacggcagttgggattcgtgaattgctgccctctggttatgtgtgggagggctaagcacaattcgagctcggtacctttaagaccaatgacttacaaggcagctgtagatcttaaccactttttaaaagaaaaggggggactggatcggtatcacttcctgtcactacctgtcggctgccgtcaaccctaagcacttaacgacgggagaccaatacacaccctcccgattcgtgttaagctcgagccatggaaattctggttactgaatgttccgtcgacatctagaattggtgaaaaattttcttttcccccctgacctBlpIBlasticidin6,3206,3406,3606,3806,4006,4206,4406,460
    agggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactcccgattaagtgagggttgcttctgttctagacgaaaaacgaacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgdeltaU3/3'LTR3' LTRdelta U36,4806,5006,5206,5406,5606,5806,6006,6206,640
    tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcaagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcatcatcaagtacagtagaataataagtcataaatattgaacgtttctttacttatagtctctcactctccttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtdeltaU3/3'LTR3' LTRSV40 pA6,6606,6806,7006,7206,7406,7606,7806,8006,820
    caaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccgagatcgatagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaaBsmISV40 pA6,8406,8606,8806,9006,9206,9406,9606,9807,000
    atttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagggacgtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccctgcatgggttaagcgggatatcactcagcataatgcgcgcgagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaSfiIAvrII7,0207,0407,0607,0807,1007,1207,1407,160
    taatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacgg7,1807,2007,2207,2407,2607,2807,3007,3207,340
    agcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgctcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgDraIII7,3607,3807,4007,4207,4407,4607,4807,5007,520
    cctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaaggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattAloI7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagSspISspIbla (amp) promoterAm...n7,7207,7407,7607,7807,8007,8207,8407,860
    aacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagattgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttctAmpicillin7,8807,9007,9207,9407,9607,9808,0008,0208,040
    acgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatAclIBcgIScaIAmpicillin8,0608,0808,1008,1208,1408,1608,1808,2008,220
    tgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccaacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtAmpicillin8,2408,2608,2808,3008,3208,3408,3608,3808,400
    cgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactAclIAmpicillin8,4208,4408,4608,4808,5008,5208,5408,560
    gcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtataAhdIAmpicillin8,5808,6008,6208,6408,6608,6808,7008,7208,740
    atactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatcttatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagapUC ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    gctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctaccgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgpUC ori8,9408,9608,9809,0009,0209,0409,0609,0809,100
    atacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatactatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatgpUC ori9,1209,1409,1609,1809,2009,2209,2409,260
    ctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattttgatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaapUC ori9,2809,3009,3209,3409,3609,3809,4009,4209,440
    tgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcPciIpUC ori9,4609,4809,5009,5209,5409,5609,5809,6009,620
    aacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcattgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtSapIBspQI9,6409,6609,6809,7009,7209,7409,7609,7809,800
    ccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaagcttggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgcgcgttaattgggagtgatttcccttgttttcgacctcgacgttcgaa9,8209,8409,8609,8809,9009,9209,940
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9951 bp700075008000850090009500500100015002000PcmvHIV-1 5' LTRRSV promoter,pRSV/5'LTR,HIV-1 psi packaging signal,BaeINruI,BspQI,SapI,NotIRRE,EcoNI,MfeIBbvCIBsmI,BsaBIKflI,PpuMI,HpaIcPPT,BspDI,ClaINdeISnaBIBsmBIEsp3IDraIII,AloISspIbla (amp) promoter,SspIAclIAmpicillin,BcgI,ScaIAclIAhdIpUC oriPciI,BspQI,SapI

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    9951