Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pHR_SaCas9
  • Sequence Map
    Editing disabled on read-only sequence.
    ctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttgactgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaNaeINgoMIVF1 ori20406080100120140160
    ccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctatggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataAloIDrdIF1 ori180200220240260280300320340
    tcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaaggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcApoIApoIFspIPsiIPvuISspILacZ alpha360380400420440460480500520
    ccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagctccaccgcggtggcggccgctctagagacggcggggagctcgctgagggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacctcgaggtggcgccaccgccggcgagatctctgccgcccctcgagcgactcEagINotISacISacIXbaIBbvCIBsmBIEsp3IEco53kIEco53kIT7M13-fwd540560580600620640660680700
    gcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacccgaactgtactaaccacgcatacaaacatacttcgatgtcctgactaaaccgcccgatactcccgcccccttcgagaccttcccggcgctaccccgcgcgccgcaggtcttccgcggtatgccgggcgaccgccgtgggtaggccatattttcgggcgctggggcttgccactggBssHIIBstEII720740760780800820840860
    tccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaggtgaaagtcgctgtttgctcgtgaatatgtatgcgctgataagacggcgatatgtattggtgagtcgatcgaattctagggtagttcgaacgtacggcccgcgcggtcttcctcgcgtcggtttggtcctactacaaactaccccataaactcgtgaacgttgggaataggccAscIBmtINheIAflIIBspEIBssHIIHindIII5´UTR HSP70ARBCS2 Promotor8809009209409609801,0001,0201,040
    aagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaaatggccaggtgagtcgacgagcaagcccggcggatcattcgggggaccgggtgtttccgatccgcggttacgttcgtcaagcgtacgtcggggacctcgccacgggaggactatttggccggtcccccggatacaagaaatgaaaaaatgttctcttcagtgagttgtagaattttaccggtccactcagctgctcgttcgggccgcctagtAccIMscISalIM1A2RBCS2 PromotorIntron 1RbcS2 Intron15´UTR RBCS25´UTR Rbcs21,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcaggatgctcgagatggcccccaagaagaagcgcaaggtgggcatccacggcgtgcccgccgccgtcgcacgaacgtctaaactgaacgttgcgggcgtaacacagctgcttccgaaaaccgaggagacagcgacagagttcgtcgtagattgggacgcagcggcaaaggtaaacgtcctacgagctctaccgggggttcttcttcgcgttccacccgtaggtgccgcacgggcggcAccISalITliIXhoIPaeR7IM1L2E3MA2PK4KK6RK8VG10IH12GV14PA161352-4702Intron 1RbcS2 Intron1SV40 NLS ...my codon1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ccaagcgcaactacatcctgggcctggacatcggcatcaccagcgtgggctacggcatcatcgactacgagacccgcgacgtgatcgacgcgggcgtgcgcctgttcaaggaggccaacgtggagaacaacgagggccgccgcagcaagcgcggcgcccgccgcctgaagcgccgggttcgcgttgatgtaggacccggacctgtagccgtagtggtcgcacccgatgccgtagtagctgatgctctgggcgctgcactagctgcgcccgcacgcggacaagttcctccggttgcacctcttgttgctcccggcggcgtcgttcgcgccgcgggcggcggacttcgcggcAK18RN20YI22LG24LD26IG28IT30SV32GY34GI36ID38YE40TR42DV44ID46AG48VR50LF52KE54AN56VE58NN60EG62RR64SK66RG68AR70RL72KR74R1352-4702SaCas91,4201,4401,4601,4801,5001,5201,5401,560
    ccgccgccaccgcatccagcgcgtgaagaagctgctgttcgactacaacctgctgaccgaccacagcgagctgagcggcatcaacccctacgaggcccgcgtgaagggcctgagccagaagctgagcgaggaggagttcagcgccgccctgctgcacctggccaagcgccgcggcggcggcggtggcgtaggtcgcgcacttcttcgacgacaagctgatgttggacgactggctggtgtcgctcgactcgccgtagttggggatgctccgggcgcacttcccggactcggtcttcgactcgctcctcctcaagtcgcggcgggacgacgtggaccggttcgcggcgccgMscIR76RH78RI80QR82VK84KL86LF88DY90NL92LT94DH96SE98LS100GI102NP104YE106AR108VK110GL112SQ114KL116SE118EE120FS122AA124LL126HL128AK130RR132G1352-4702SaCas91,5801,6001,6201,6401,6601,6801,7001,7201,740
    gtgcacaacgtgaacgaggtggaggaggacaccggcaacgagctgtccaccaaggagcagatcagccgcaacagcaaggccctggaggagaagtacgtggccgagctgcagctggagcgcctgaagaaggacggcgaggtgcgcggcagcatcaaccgcttcaagaccagcgactcacgtgttgcacttgctccacctcctcctgtggccgttgctcgacaggtggttcctcgtctagtcggcgttgtcgttccgggacctcctcttcatgcaccggctcgacgtcgacctcgcggacttcttcctgccgctccacgcgccgtcgtagttggcgaagttctggtcgctgaPstIV134HN136VN138EV140EE142DT144GN146EL148ST150KE152QI154SR156NS158KA160LE162EK164YV166AE168LQ170LE172RL174KK176DG178EV180RG182SI184NR186FK188TS190D1352-4702SaCas91,7601,7801,8001,8201,8401,8601,8801,9001,920
    acgtgaaggaggcgaagcagctgctgaaggtgcagaaggcctaccaccagctggaccagagcttcatcgacacctacatcgacctgctggagacccgccgcacctactacgagggccccggcgagggcagccccttcggctggaaggacatcaaggagtggtacgagatgctgattgcacttcctccgcttcgtcgacgacttccacgtcttccggatggtggtcgacctggtctcgaagtagctgtggatgtagctggacgacctctgggcggcgtggatgatgctcccggggccgctcccgtcggggaagccgaccttcctgtagttcctcaccatgctctacgactaApaIStuIPflMIPspOMIY192VK194EA196KQ198LL200KV202QK204AY206HQ208LD210QS212FI214DT216YI218DL220LE222TR224RT226YY228EG230PG232EG234SP236FG238WK240DI242KE244WY246EM248LM2501352-4702SaCas91,9401,9601,9802,0002,0202,0402,0602,0802,100
    gggccactgcacctacttccccgaggagctgcgctccgtgaagtacgcctacaacgccgacctgtacaacgccctgaacgacctgaacaacctggtcatcacccgcgacgagaacgagaagctggagtactacgagaagttccagatcatcgagaacgtgttcaagcagaagaagcccggtgacgtggatgaaggggctcctcgacgcgaggcacttcatgcggatgttgcggctggacatgttgcgggacttgctggacttgttggaccagtagtgggcgctgctcttgctcttcgacctcatgatgctcttcaaggtctagtagctcttgcacaagttcgtcttcttcSexAIGH252CT254YF256PE258EL260RS262VK264YA266YN268AD270LY272NA274LN276DL278NN280LV282IT284RD286EN288EK290LE292YY294EK296FQ298II300EN302VF304KQ306KK3081352-4702SaCas92,1202,1402,1602,1802,2002,2202,2402,260
    aagcccaccctgaagcagatcgccaaggagatcctggtcaacgaggaggacatcaagggctaccgcgtgaccagcaccggcaagcccgagttcaccaacctgaaggtgtaccacgacatcaaggacatcaccgcccgcaaggagatcatcgagaacgccgagctgctggaccagattcgggtgggacttcgtctagcggttcctctaggaccagttgctcctcctgtagttcccgatggcgcactggtcgtggccgttcgggctcaagtggttggacttccacatggtgctgtagttcctgtagtggcgggcgttcctctagtagctcttgcggctcgacgacctggtctKP310TL312KQ314IA316KE318IL320VN322EE324DI326KG328YR330VT332ST334GK336PE338FT340NL342KV344YH346DI348KD350IT352AR354KE356II358EN360AE362LL364DQ3661352-4702SaCas92,2802,3002,3202,3402,3602,3802,4002,4202,440
    tcgcgaagatcctgaccatctaccagagcagcgaggacatccaggaggagctgaccaacctgaactccgagctgacccaggaggagatcgagcagatctcgaacctgaagggctacaccggcacccacaacctgagcctgaaggccatcaacctgatcctggacgagctgtggcaagcgcttctaggactggtagatggtctcgtcgctcctgtaggtcctcctcgactggttggacttgaggctcgactgggtcctcctctagctcgtctagagcttggacttcccgatgtggccgtgggtgttggactcggacttccggtagttggactaggacctgctcgacaccgtNruIPfoIPfoIBglIIIA368KI370LT372IY374QS376SE378DI380QE382EL384TN386LN388SE390LT392QE394EI396EQ398IS400NL402KG404YT406GT408HN410LS412LK414AI416NL418IL420DE422LW424H1352-4702SaCas92,4602,4802,5002,5202,5402,5602,5802,6002,620
    caccaacgacaaccagatcgccatcttcaaccgcctgaagctggtgcccaagaaggtggacctgtcccagcagaaggagatccccaccaccctggtggacgacttcatcctgagccccgtcgtgaagcgcagcttcatccagagcatcaaggtgatcaacgccatcatcaagaaggtggttgctgttggtctagcggtagaagttggcggacttcgaccacgggttcttccacctggacagggtcgtcttcctctaggggtggtgggaccacctgctgaagtaggactcggggcagcacttcgcgtcgaagtaggtctcgtagttccactagttgcggtagtagttcttcBsmFIT426ND428NQ430IA432IF434NR436LK438LV440PK442KV444DL446SQ448QK450EI452PT454TL456VD458DF460IL462SP464VV466KR468SF470IQ472SI474KV476IN478AI480IK482K1352-4702SaCas92,6402,6602,6802,7002,7202,7402,7602,7802,800
    tacggcctgcccaacgacatcatcatcgagctggcccgcgagaagaactccaaggacgcccagaagatgatcaacgagatgcagaagcgcaaccgccagaccaacgagcgcatcgaggagatcatccgcaccaccggcaaggagaacgccaagtacctgatcgagaagatcaagcatgccggacgggttgctgtagtagtagctcgaccgggcgctcttcttgaggttcctgcgggtcttctactagttgctctacgtcttcgcgttggcggtctggttgctcgcgtagctcctctagtaggcgtggtggccgttcctcttgcggttcatggactagctcttctagttcgY484GL486PN488DI490II492EL494AR496EK498NS500KD502AQ504KM506IN508EM510QK512RN514RQ516TN518ER520IE522EI524IR526TT528GK530EN532AK534YL536IE538KI540K1352-4702SaCas92,8202,8402,8602,8802,9002,9202,9402,960
    tgcacgacatgcaggagggcaagtgcctgtactccctggaggccatcccgctggaggacctgctgaacaaccccttcaactacgaggtggaccacatcatcccccgcagcgtgtccttcgacaacagcttcaacaacaaggtgctggtcaagcaggaggagaacagcaagaagggacgtgctgtacgtcctcccgttcacggacatgagggacctccggtagggcgacctcctggacgacttgttggggaagttgatgctccacctggtgtagtagggggcgtcgcacaggaagctgttgtcgaagttgttgttccacgaccagttcgtcctcctcttgtcgttcttcccPpuMIBstAPIL542HD544MQ546EG548KC550LY552SL554EA556IP558LE560DL562LN564NP566FN568YE570VD572HI574IP576RS578VS580FD582NS584FN586NK588VL590VK592QE594EN596SK598KG6001352-4702SaCas92,9803,0003,0203,0403,0603,0803,1003,1203,140
    caaccgcaccccgttccagtacctgagcagcagcgacagcaagatcagctacgagaccttcaagaagcacatcctgaacctggcgaagggcaagggccgcatctccaagaccaagaaggagtacctgctggaggagcgcgacatcaaccgcttctccgtgcagaaggacttcatcgttggcgtggggcaaggtcatggactcgtcgtcgctgtcgttctagtcgatgctctggaagttcttcgtgtaggacttggaccgcttcccgttcccggcgtagaggttctggttcttcctcatggacgacctcctcgcgctgtagttggcgaagaggcacgtcttcctgaagtagNR602TP604FQ606YL608SS610SD612SK614IS616YE618TF620KK622HI624LN626LA628KG630KG632RI634SK636TK638KE640YL642LE644ER646DI648NR650FS652VQ654KD656FI6581352-4702SaCas93,1603,1803,2003,2203,2403,2603,2803,3003,320
    aaccgcaacctggtggacacccgctacgccacccgcggcctgatgaacctgctgcgctcgtacttccgcgtgaacaacctggacgtgaaggtgaagtccatcaacggcggcttcacctcgttcctgcgccgcaagtggaagttcaagaaggagcgcaacaagggctacaagcaccttggcgttggaccacctgtgggcgatgcggtgggcgccggactacttggacgacgcgagcatgaaggcgcacttgttggacctgcacttccacttcaggtagttgccgccgaagtggagcaaggacgcggcgttcaccttcaagttcttcctcgcgttgttcccgatgttcgtggSexAINR660NL662VD664TR666YA668TR670GL672MN674LL676RS678YF680RV682NN684LD686VK688VK690SI692NG694GF696TS698FL700RR702KW704KF706KK708ER710NK712GY714KH7161352-4702SaCas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    acgccgaggacgccctgatcatcgccaacgccgacttcatcttcaaggagtggaagaagctggacaaggccaagaaggtgatggagaaccagatgttcgaggagaagcaggccgagagcatgcccgagatcgagaccgagcaggagtacaaggagatcttcatcaccccccaccatgcggctcctgcgggactagtagcggttgcggctgaagtagaagttcctcaccttcttcgacctgttccggttcttccactacctcttggtctacaagctcctcttcgtccggctctcgtacgggctctagctctggctcgtcctcatgttcctctagaagtagtggggggtggtAjuIBglIIHA718ED720AL722II724AN726AD728FI730FK732EW734KK736LD738KA740KK742VM744EN746QM748FE750EK752QA754ES756MP758EI760ET762EQ764EY766KE768IF770IT772PH774Q1352-4702SaCas93,5203,5403,5603,5803,6003,6203,6403,660
    gatcaagcacatcaaggacttcaaggactacaagtacagccaccgcgtggacaagaagccgaaccgcgagctgatcaacgacaccctgtacagcacccgcaaggacgacaagggcaacaccctgatcgtgaacaacctgaacggcctgtacgacaaggacaacgacaagctgaagctagttcgtgtagttcctgaagttcctgatgttcatgtcggtggcgcacctgttcttcggcttggcgctcgactagttgctgtgggacatgtcgtgggcgttcctgctgttcccgttgtgggactagcacttgttggacttgccggacatgctgttcctgttgctgttcgacttcPflMII776KH778IK780DF782KD784YK786YS788HR790VD792KK794PN796RE798LI800ND802TL804YS806TR808KD810DK812GN814TL816IV818NN820LN822GL824YD826KD828ND830KL832K1352-4702SaCas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    aagctgatcaacaagagccccgagaagctgctgatgtaccaccacgacccccagacctaccagaagctgaagctgatcatggagcagtacggcgacgagaagaaccccctgtacaagtactacgaggagaccggcaactacctgaccaagtactccaagaaggacaacggccccgttcgactagttgttctcggggctcttcgacgactacatggtggtgctgggggtctggatggtcttcgacttcgactagtacctcgtcatgccgctgctcttcttgggggacatgttcatgatgctcctctggccgttgatggactggttcatgaggttcttcctgttgccggggcK834LI836NK838SP840EK842LL844MY846HH848DP850QT852YQ854KL856KL858IM860EQ862YG864DE866KN868PL870YK872YY874EE876TG878NY880LT882KY884SK886KD888NG890P1352-4702SaCas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    tgatcaagaagatcaagtactacggcaacaagctgaacgcccacctggacatcaccgacgactaccccaacagccgcaacaaggtcgtgaagctgtccctgaagccctaccgcttcgacgtgtacctggacaacggcgtgtacaagttcgtgaccgtgaagaacctggacgtgatactagttcttctagttcatgatgccgttgttcgacttgcgggtggacctgtagtggctgctgatggggttgtcggcgttgttccagcacttcgacagggacttcgggatggcgaagctgcacatggacctgttgccgcacatgttcaagcactggcacttcttggacctgcactaBsmFIV892IK894KI896KY898YG900NK902LN904AH906LD908IT910DD912YP914NS916RN918KV920VK922LS924LK926PY928RF930DV932YL934DN936GV938YK940FV942TV944KN946LD948VI9501352-4702SaCas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    caagaaggagaactactacgaggtgaacagcaagtgctacgaggaggccaagaagctgaagaagatcagcaaccaggccgagttcatcgcctcgttctacaacaacgacctgatcaagatcaacggcgagctgtaccgcgtgatcggcgtgaacaacgacctgctgaaccgcatcgttcttcctcttgatgatgctccacttgtcgttcacgatgctcctccggttcttcgacttcttctagtcgttggtccggctcaagtagcggagcaagatgttgttgctggactagttctagttgccgctcgacatggcgcactagccgcacttgttgctggacgacttggcgtagKK952EN954YY956EV958NS960KC962YE964EA966KK968LK970KI972SN974QA976EF978IA980SF982YN984ND986LI988KI990NG992EL994YR996VI998GV1000NN1002DL1004LN1006RI10081352-4702SaCas94,2204,2404,2604,2804,3004,3204,3404,360
    gaggtgaacatgatcgacatcacctaccgcgagtacctggagaacatgaacgacaagcgccccccccgcatcatcaagaccatcgccagcaagacccagtcgatcaagaagtacagcaccgacatcctgggcaacctgtacgaggtgaagtcgaagaagcacccccagatcatcactccacttgtactagctgtagtggatggcgctcatggacctcttgtacttgctgttcgcggggggggcgtagtagttctggtagcggtcgttctgggtcagctagttcttcatgtcgtggctgtaggacccgttggacatgctccacttcagcttcttcgtgggggtctagtagtPflFITth111IEV1010NM1012ID1014IT1016YR1018EY1020LE1022NM1024ND1026KR1028PP1030RI1032IK1034TI1036AS1038KT1040QS1042IK1044KY1046ST1048DI1050LG1052NL1054YE1056VK1058SK1060KH1062PQ1064II10661352-4702SaCas94,3804,4004,4204,4404,4604,4804,5004,5204,540
    agaagggcagcggcaagcgccccgcggccaccaagaaggccggccaggccaagaagaagaagggcagctacccctacgacgtgcccgactacgcctacccgtacgacgtgccggactacgcgtacccctacgacgtgccggactacgcctaaggatccccgctccgtgtaaatggtcttcccgtcgccgttcgcggggcgccggtggttcttccggccggtccggttcttcttcttcccgtcgatggggatgctgcacgggctgatgcggatgggcatgctgcacggcctgatgcgcatggggatgctgcacggcctgatgcggattcctaggggcgaggcacatttaccFseIMluINaeISfiIBamHIBsiWIBsu36INgoMIVKK1068GS1070GK1072RP1074AA1076TK1078KA1080GQ1082AK1084KK1086KG1088SY1090PY1092DV1094PD1096YA1098YP1100YD1102VP1104DY1106AY1108PY1110DV1112PD1114YA1116*1352-4702NLSRbcs2\Term.4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgatttccgcgagcaactagactcggaacgggggactgcttgccgccacctaccttctatgacgagagttcacgacttcgccatcgaatcgaggggcaaagcacgactagtcagaaaaagttgtgcatttttcgcctcctcaaaacgttaaaacaaccaacattgctaggaggcaactaaRbcs2\Term.Terminator Hairpin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ttggcctctttctccatgggcgggctgggcgtatttgaagcgggtaccagcttttgttccctttagtgagggttaatttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaaccggagaaagaggtacccgcccgacccgcataaacttcgcccatggtcgaaaacaagggaaatcactcccaattaaagctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatKpnINcoIAcc65IRbcs2\Term.lac promoterLacOM13-revT3T3 promoter4,9204,9404,9604,9805,0005,0205,0405,060
    aagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcSapITfiIBspQI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgPciITfiIColE1 origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgaDrdIBciVIBssSIBssSαIColE1 origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaggtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtcColE1 origin5,6205,6405,6605,6805,7005,7205,7405,760
    agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaColE1 origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttBspHIColE1 origin5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgcAhdIBsrDIAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    agacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcttctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgaAclIFspIBsrDIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    acaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggtgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccPvuIAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtAclIBssSIBssSαI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtgSspIBciVIBspHI7,0207,0407,0607,0807,1007,1207,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7148 bp500055006000650050010001500SaCas9D10A1352-4702Intron 3 F (DNA)Intron 3 R (DNA)HR F (DNA)

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    7148