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pBT13-splitD (1st generation M13 backbone donor)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cttattatcaAccggggTacatatgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagacctctcaaaaatagctaccctctccggcatgaatttatcagctagaacggttgaatatcatgttgaataatagtTggccccAtgtatactaactgtacgatcaaaatgctaatggcaagtagctaagagaacaaacgaggtctgagagtccgttactggactatcggaaacatctggagagtttttatcgatgggagaggccgtacttaaatagtcgatcttgccaacttatagtacaaAccIBpmIClaINdeISapIBspDIBspQIBsrDIgIIgI...S20406080100120140160
    gatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgattctaccactaaactgacagaggccggaaagagtgggaaaacttagaaatggatgtgtaatgagtccgtaacgtaaattttatatactcccaagatttttaaaaataggaacgcaactttatttccgaagagggcgttttcataatgtcccagtattacaaaaaccatgttggctaaBsrDIgIIgII' promoter (internal)180200220240260280300320340
    tagttttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccgccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaaatcaaaatacgagactccgaaataacgaattaaaacgattaagaaacggaacggacatactaaataacctacaattgcgatgatgataatcatcttaactacggcggaaaagtcgagcgcggggtttacttttatatcgatttgtccaataactggtaaacgctttacatagattHpaIHincIIgII360380400420440460480500520
    tggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaccagtttgatttagatgagcaagcgtcttaacccttagttgacaatgtaccttactttgaaggtctgtggcatgaaatcaacgtataaattttgtacaactcgatgtcgtggtctaagtcgttaattcgagattcggtaggcgtttttactggagaatagttttcctcgttaatPciIgII540560580600620640660680700
    aaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtttccatgagagattaggactggacaacctcaaacgaaggccagaccaagcgaaacttcgagcttaattttgcgctataaacttcagaaagcccgaaggagaattagaaaaactacgttaggcgaaacgaagactgatattatcagtcccatttctggactaaaaactaaataccaXmnIgII720740760780800820840860
    cattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagtaagagcaaaagacttgacaaatttcgtaaactccccctaagttacttataaatactgctaaggcgtcataacctgcgataggtcagatttgtaaaatgataatgggggagaccgttttgaagaaaacgttttcggagagcgataaaaccaaaaatagcagcagaccatttgctgIIgX8809009209409609801,0001,0201,040
    gggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggcccaatactatcacaacgagaatgatacggagcattaaggaaaaccgcaatacatagacgtaatcaacttacaccataaggatttagagttgactacttagaaagatggacattattacaacaaggcaatcaagcaaaataattgcatctaaaaagaagggttgcaggactgaccgIIgXgV promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtatattactcggtcaagaattttagcgtattccattaagtgttactaatttcaactttaatttggtagagttcgggttaaatgatgagcaagaccacaaagagcagtcccgttcggaataagtgacttactcgtcgaaacaatgcaactaaacccattacttataggccaagaacagVgV promotergIIgXgV RBS1,2401,2601,2801,3001,3201,3401,3601,3801,400
    caagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatgttctaatgagaactacttccagtcggtcggatacgcggaccagacatgtggcaagtagacaggagaaagtttcaaccagtcaagccaagggaatactaactggcagacgcggagcaaggccgattcattgtacctcgtccagcgcctaaagctgtgttaaatagtccgctactaTatIBfuAIBspMIBsrGIgVgVII1,4201,4401,4601,4801,5001,5201,5401,560
    acaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagctgtttagaggcaacatgaaacaaagcgcgaaccatattagcgacccccagtttctactcacaaaatcacataagaaagcggagaaagcaaaatccaaccacggaagcatcaccgtaatgcataaaatgggcaaattacctttgaaggagtactttttcagaaatcaggagtttcgTatIBsaAIBseYIBtgZISnaBIgIXgVIIgVIII promotergVIIIgVI...BS1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaagagacatcggcaacgatgggagcaaggctacgacagaaagcgacgactcccactgctagggcgttttcgccggaaattgagggacgttcggagtcgctggcttatatagccaatacgcacccgctaccaacaacagtaacagccgcgttgatagccatagttcgacaaattctttBsiEIBtgZIEcoP15IgVIII1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagccttttttttggagtAgaagagcTccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgaatctgaaagaggagaaaggatctatggcgagtagcgaagacgtaagtggagctttcgttcgactatttggctatgttaatttccgaggaaaacctcggaaaaaaaacctcaTcttctcgAgggatagtcactatctctaactgtagggatagtcactatctctatgactcgtgcttagactttctcctctttcctagataccgctcatcgcttctgcaSacISapIBanIIBspQIEco53kItet promotergIII promoterterminator 1gVIIImRFP-101,9401,9601,9802,0002,0202,0402,0602,0802,100
    tatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcatagtttctcaagtacgcaaagtttcaagcataccttccaaggcaattgccagtgctcaagctttagcttccacttccacttccagcaggcatgcttccatgggtctggcgatttgactttcaatggtttccaccaggcgacggcaagcgaaccctgtaggacaggggcgtcaagAhdIBaeIHpaIKpnIPsrIBsiWIBssSIBstBIAcc65IBssSαIHincIImRFP2,1202,1402,1602,1802,2002,2202,2402,260
    cagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaacgtcatgccaaggtttcgaatgcaatttgtgggccgactgtagggcctgatggactttgacaggaagggccttccaaagtttacccttgcacaatacttgaagcttctgccaccacaacaatggcaatgggtcctgaggagggacgttctgccactcaagtagatgtttcaatttgPfoIPfoIBstBIHindIIImRFP2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatacgcaccatggttgaagggcaggctgccaggccaatacgtctttttttggtacccaacccttcgaaggtggcttgcatacatgggccttctgccacgagactttccactttagttttacgcagactttgactttctgccaccagtgatgctgcgacttcaattttggtggatgtaBaeIBtgIKpnINcoIStyIBstXIPflMIPshAIRsrIIAcc65IHindIIImRFP2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaggatctccaggcatcaaataaaacgaaaggctcagtcgaccgattttttggccaagtcgacggcccacgaatgttttggctgtagtttgacctgtagtggagggtgttgcttctgatgtggtagcaacttgtcatgcttgcacgacttccagcagtgaggtggccacgaattattcctagaggtccgtagtttattttgctttccgagtcagctAcuIAcuIAgeIAgeIBpmIBsaXIPvuIISgrAImRFPterminatorrrnB1 rho...rminatorstem loopT-rich2,6402,6602,6802,7002,7202,7402,7602,7802,800
    aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttAtacaggtggcactttTcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgagtggaagcccacccggaaagacgcaaaTatgtccaccgtgaaaAgcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgaPsrIBsrBIterminatorrrnB1 r...inatorUSER ...tionstem loop2,8202,8402,8602,8802,9002,9202,9402,960
    catgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcggtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcAseIXmnIBciVIPflMIaph (KanR)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggAseINsiIaph (KanR)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggccAjuIAvaIBsmIBsmIPvuISmaIXmaIAsiSIBsiEIBsmBIBsoBIEcoNIEsp3ITspMIaph (KanR)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatNsiIaph (KanR)3,5203,5403,5603,5803,6003,6203,6403,660
    caatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaaClaINruIBanIIBspDIaph (KanR)NOT NECESSARILY SUFF... context-dependent?3,6803,7003,7203,7403,7603,7803,8003,8203,840
    catgaAggccaaataggccgTtcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcagtactTccggtttatccggcAagtctaggaaggcataaatcggtcatacaagagatcacaccaagcaacaaaaacgcactcggtactcttgcttggtaactctagtacgaatgaaacgtacagtgagtttttaaaacggagttttgaccactcgacttaaaaacgtcaatttcgtBglISfiI*I316RG314YK312AL310IN308EL306TT304RQ302KQ300TL298WS296FS294GN292LD290HK288SQ286MD284SL282FK280AE278FS276TL274QI272KA270TL268A3872-4822repAUSER ...tion3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaagcacatcacaaaaagaatcaggcaatgcatccatccttagactacattaccaacaaccataaaacagtggtaagtaaaaatagaccaacaagagttcaagccaatgctctaggtaaacagatagatcaagttgaaccttttagttgcatagtcagcccgccggagcgaatagttBsaAISnaBID266HL264TK262RL260GN258RL256YS254DS252TI250TT248PI246KD244GN242MK240IQ238NN236EL234ET232VL230DM228QR226DL224EV222QF220IL218TD216TP214RG212RK210DV2083872-4822repA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaaggtggttaaagtataacgacattcacaaatttagaaatgaataaccaaagttttgggtaaccaattcggaaaatttgagtaccatcaataaaagttcgtaattgtacttgaatttaagtagttccgattagagatataaacggaacactcaaaagaaaacacaatcaagaaaattVL206KM204NS202YT200NL198DK196SI194PK192LV190WQ188NL186RK184FE182HY180NN178EL176ML174MF172KF170ED168LS166IE164IN162AK160HT158KK156QT154LE152KL1503872-4822repA4,2204,2404,2604,2804,3004,3204,3404,360
    taaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagattggtgagtatttaggagtatctcataaacaaaagttttctgaattgtacaaggtctaatataaaatacttaaaaaaattgaccttttctattccgttatagagaagtgatttttgattaagattaaaaagcgaactcttgaaccgtatcaaacaggtgaccttttagagtttcPciILW148EY146IR144MS142YK140NE138FS136KV134HE132LN130YK128IF126KK124LQ122FL120YP118LI116EE114SF112VL110EL108KE106SS104FK102AY100NT98WQ96FI94EF923872-4822repA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtaggaaattggtttcctaaggactaaaggtgtcaagagcagtagtcgagagaccaacgaaatcgattatgtggtattcgtaaaagggatgactacaagtagtagactcgcataaccaatattcacttgctatggcaggcaagaaaggaacatcccaaaagttagcaccccaactcatPsiIGK90VL88PN86RI84EV82TR80TM78LE76RT74AK72AL70VG68YA66NE64RS62IN60MM58QA56YQ54NY52TF50SV48TR46EK44RT42PN40EI38TP36NL34L3872-4822repA4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcacggtgtgtcgtattttaatcgaaccaaagtacgaggcaattcagtatcgctgattagcgatcaagtaaacgaaacttttgttgattaagtctgtatgtagagttaaccagatccactaaaattagtgatatggttaactctacccgatcagttactattaatgatcaggaaaaMfeIMfeISpeIA32VC30CL28IL26KT24EH22ET20LD18YR16SI14AL12EN10AK8FV6VL4ES2M3872-4822repApSC101 ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccggaaactcaacacccatagacatttaagacgatctggaaacgaccttttgaacatttaagacgatctgggagacatttaaggcgatctggaaacacacaaaaaaaacaaatataagttcaccaatattaaatatcttatttctttcttatttttttctatttttcttatctagggPsiIBseYIpSC101 ori4,9204,9404,9604,9805,0005,0205,0405,060
    agccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgtcgggacacatattgagtgatgaaatcagtcaaggcgtcataatgttttcctacagcgtttgcgacaaacgaggagatgttttgtctggaattttgggatttccgaattcatcgtgggagcgttcgagcccgtttagcgacttataaggaaaacagaggctggtagtccgtggacAvaIAflIIBsoBIpSC101 ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    agtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgtcagcgacagaaaaagcactgtaagtcaagcgacgcgagtgccgagaccgtcacttacccccatttaccgtgatgtccgcggaaaatacctaagtacgttcctttgatgggtattatgttcttttcgggcagtgcccgaagagtcccgcaaaataccgcccagacgatacaccacBcgIKasINarISfoIBsaHIHaeIIPluTIpSC101 oripartitioning locus, gyrase bindinghairpin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtcaagaggacatccggtcaaataaaacgaaagatagactgaaaaacgacaagtcgtcaaggacgggagactaaaaggtcagactggtgaagcctaatagggcactgtccagtaagtctgaccgattacgtgggtcattccgtcgccatagtagttgtccgaatgggcagaatgacagttctcctgtaggccagtttattttgctttAhdIAlwNIBstAPIEcoP15IpSC101 oripartitionin...se bindingUSER...ionrrnB...torT-rich5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ggctcagtcgaaagactgggcctttcgttttgctgaggagacttagggaccctgctcttctccgagtcagctttctgacccggaaagcaaaacgactcctctgaatccctgggacgagaagaKflIPpuMIEcoO109IrrnB1 rho-in... terminatorstem loop5,6205,6405,660
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    5661 bpSapI Golden Gate dropout cassette: ori, KmR, RFP400042504500475050005250550025050075010001250repAgVgV RBSgV promotergIIgX3872-4822CTT for SapI Golden Gate cloning,gII RBS,gII' promoter (internal),BspQI,SapI,+7HincIIHpaIPciIXmnIPciIPsiIMfeIMfeI,SpeIPsiIBseYI,AflIIpSC101 ori,partitioning locus, gyrase binding,AvaI,BsoBI,BcgI,KasI,BsaHI,+4hairpin,USER junction,rrnB1 rho-independent transcriptional terminator,T-rich,+10

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    5661