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U6.2-SaCas9_cloning
  • Sequence Map
    Editing disabled on read-only sequence.
    acacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatatttttgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaaSspISspIBciVIBspHICspCI20406080100120140160
    gttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgcttttAloIDrdIPsiIBsaXIF1 ori180200220240260280300320340
    accgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcggtggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgccNaeIBsaAIBtgZIDraIIINgoMIVF1 ori360380400420440460480500520
    gcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgBglIPvuIBsmFIPvuIILacZ alphaF1 ori540560580600620640660680700
    tgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagctccaccgcggtggcggccgctctagaactagtggatcccccgggctgcAATGGTGTTGAGAAAAGGCTACAacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacctcgaggtggcgccaccgccggcgagatcttgatcacctagggggcccgacgTTACCACAACTCTTTTCCGATGTAleIBtgIEagINotISacISmaISpeIXbaIXmaIBamHIBstXISacIITspMIEco53kIT7PU6.2Fragme...ion))M13-fwd720740760780800820840860
    GTCAGTGGCGGGTTTCAGGAATGCCGGTTTTTCCCTCGCGCTTCCCAGCCATGCCAGCTAAATGCCTGGGTATTGGGATTGATAATCCAGTTGATACCTGGCAGCAGGGCAGCGCGCGGTGGGTAGCTGCTCCCCTCGCGCATTGCATGGGTGCAAATTCATGCTTTTGCGCAGGCAGTCACCGCCCAAAGTCCTTACGGCCAAAAAGGGAGCGCGAAGGGTCGGTACGGTCGATTTACGGACCCATAACCCTAACTATTAGGTCAACTATGGACCGTCGTCCCGTCGCGCGCCACCCATCGACGAGGGGAGCGCGTAACGTACCCACGTTTAAGTACGAAAACGCGTCCBsmIXcmIPU6.28809009209409609801,0001,0201,040
    GAGCAGGATGCCCTCATAAATCCTACGCGCGCAAGGTTGGGAGTCATTGCCCACCCGTACCCCAACAAGCTATGCATACCCAAGCTGCCACACCACTGGGCTGTCGCCGGCAACCAATGGCGCGAAAGCTGGCTTGCTGTCACAATGGGCCTTTTTCCCAGCCTCGCAGCGTGGGCTCGTCCTACGGGAGTATTTAGGATGCGCGCGTTCCAACCCTCAGTAACGGGTGGGCATGGGGTTGTTCGATACGTATGGGTTCGACGGTGTGGTGACCCGACAGCGGCCGTTGGTTACCGCGCTTTCGACCGAACGACAGTGTTACCCGGAAAAAGGGTCGGAGCGTCGCACCCNaeINsiIBstXINgoMIVPU6.21,0601,0801,1001,1201,1401,1601,1801,2001,220
    CGTGGCTACCTAGAACCCGCATGCACGCTGGAGAGCGCCCAGGAACATCTGCGCTATGGAAGGGGCCTTGGGGCTACTGTTCCACGATGGGACTTACCGGGTCGGGATCAACGCGCTCCCGCGCGGGAAAGGTCGGGCTGGCGCCGGTGTCTGTTTGGACGCGACTTTTGCGCCCGCACCGATGGATCTTGGGCGTACGTGCGACCTCTCGCGGGTCCTTGTAGACGCGATACCTTCCCCGGAACCCCGATGACAAGGTGCTACCCTGAATGGCCCAGCCCTAGTTGCGCGAGGGCGCGCCCTTTCCAGCCCGACCGCGGCCACAGACAAACCTGCGCTGAAAACGCGGGBpmIKasINarINspISfoISphIStyIBsaHIBsmFIPluTISgrAIEcoO109IPU6.21,2401,2601,2801,3001,3201,3401,3601,3801,400
    TAGTGATATGTATATTACACCGACGAGCACTactttgagacgtaccatatgaaccgtctccgttttagtactctggaaacagaatctactaaaacaaggcaaaatgccgtgtttatctcgtcaacttgttggcgagattttttcccgggCTGCAGGAATTCGAtatcaagcttatATCACTATACATATAATGTGGCTGCTCGTGAtgaaactctgcatggtatacttggcagaggcaaaatcatgagacctttgtcttagatgattttgttccgttttacggcacaaatagagcagttgaacaaccgctctaaaaaagggcccGACGTCCTTAAGCTatagttcgaataNdeIPstIScaISmaITatIXmaIBsmBIBsmBIEcoRIEcoRVEsp3IEsp3ITspMIHincIIHindIIISaScaffoldPU6.2Cloning InsertFr...)1,4201,4401,4601,4801,5001,5201,5401,560
    cgataccgtcgacctcgagggggggcccggtacccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcgctatggcagctggagctcccccccgggccatgggtcgaaaacaagggaaatcactcccaattaacgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacgAccIApaIClaIKpnISalITliIXhoIBspDIPspXIAcc65IHincIIPaeR7IPspOMIEcoO109Ilac promoterLacOM13-revT3T3 promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcgggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccSapIBsaXIBspQIPvuII1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaagacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgttttNspIPciIAflIIIColE1 origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatDrdIBciVIBssSIBssSαIColE1 origin2,1202,1402,1602,1802,2002,2202,2402,260
    tctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatAlwNIApaLIColE1 origin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagaccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctAcuIColE1 origin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatataaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatBspHIColE1 origin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtAhdIBpmIBsaIAmpR2,8202,8402,8602,8802,9002,9202,9402,960
    ccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccAclIBglIAvaIINmeAIIIAmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataaacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattPvuIAvaIIAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttctaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaAclIBcgIScaITatIXmnIBsaHIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgcAcuIApaLIBssSIBssSαI3,5203,5403,5603,5803,6003,6203,640
    • Plasmid
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    Editing disabled on read-only sequence.
    3659 bp25002750300032503500250500750PU6.2AmpRp044_PU6.2_(pJET,_840) FWD (DNA)p044_PU6.2_(pJET,_840) REV (DNA)

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    3659