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pBZcsx27
  • Sequence Map
    Editing disabled on read-only sequence.
    tctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagaAcuIBceAIRNAII transcript (complementary strand)RNAI transcriptpUC19pMB1 origin of replication20406080100120140160
    gtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtMmeIAlwNIBseYIRNAII transcript (complementary strand)pUC19pMB1 origin of replication180200220240260280300320340
    ccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgactggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaMmeIBciVIRNAII transcript (complementary strand)pUC19pMB1 origin of replication360380400420440460480500520
    tgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggDrdIPciIAflIIIRNAII transcript (complementary strand)pUC19pMB1 origin of replication540560580600620640660680700
    gctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagccgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgSapIBsaXIBspQIPvuIICA...epUC19720740760780800820840860
    tcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcgactctagaggatccTTTACACTTTATGCTTCCGGCTCGTAagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgaacgtacggacgtccagctgagatctcctaggAAATGTGAAATACGAAGGCCGAGCATAccIPstISalISbfISphIXbaIBamHIBfuAIBspMIHincIIHindIIILac promoterlac rep...g sitepUC198809009209409609801,0001,0201,040
    TGTTAGGAGGTCTTTATCatgattgagctgataaatttttcagcggaatatgtttttgggaaagccccactcattacagcaggaaaccttagcgacggtctgttgttttttgccattgccctatttataggatgcttgattcatatcatcacattttggttgataaagagaaagtACAATCCTCCAGAAATAGtactaactcgactatttaaaaagtcgccttatacaaaaaccctttcggggtgagtaatgtcgtcctttggaatcgctgccagacaacaaaaaacggtaacgggataaatatcctacgaactaagtatagtagtgtaaaaccaactatttctctttcaAjuIBsaBIBpu10IMI2EL4IN6FS8AE10YV12FG14KA16PL18IT20AG22NL24SD26GL28LF30FA32IA34LF36IG38CL40IH42II44TF46WL48IK50RK521069-1674bzcsx27pUC191,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggtataagaaattggcttgtacgcctatggacaaaatgaaaaatgacgagtacatcactgaaattcttcctgatttgaaagaaatttacagaacaaataaaggaatttcggtaacagaaaatatcagtaatggaagtgtctttgatacagcgtattattatttagaggcacaaggccatattctttaaccgaacatgcggatacctgttttactttttactgctcatgtagtgactttaagaaggactaaactttctttaaatgtcttgtttatttccttaaagccattgtcttttatagtcattaccttcacagaaactatgtcgcataataataaatctccgtgttccXmnIWY54KK56LA58CT60PM62DK64MK66ND68EY70IT72EI74LP76DL78KE80IY82RT84NK86GI88SV90TE92NI94SN96GS98VF100DT102AY104YY106LE108AQ110G1069-1674bzcsx27pUC191,2401,2601,2801,3001,3201,3401,3601,3801,400
    caaaatatcccaagcaaaaaattttcagagtatttatttcttgtttcggaacatcgtaacgctttcgttatttgtacttcccgtgagtgttatctttttattagcatccttttttatgaatgattgttcgctgagtgagaaaatcattacaattattataggaaccttcgttctagttttatagggttcgttttttaaaagtctcataaataaagaacaaagccttgtagcattgcgaaagcaataaacatgaagggcactcacaatagaaaaataatcgtaggaaaaaatacttactaacaagcgactcactcttttagtaatgttaataatatccttggaagcaagatK112IS114QA116KN118FQ120SI122YF124LF126RN128IV130TL132SL134FV136LP138VS140VI142FL144LA146SF148FM150ND152CS154LS156EK158II160TI162II164GT166FV168L1069-1674bzcsx27pUC191,4201,4401,4601,4801,5001,5201,5401,560
    ggtggattatcatcggttatagcccaatggtttcgggtaaaaatgacagacagaattttcggactttactatgcagaattaacacacaataaaaaataaccgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttccacctaatagtagccaatatcgggttaccaaagcccatttttactgtctgtcttaaaagcctgaaatgatacgtcttaattgtgtgttattttttattggcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaaAloIBmrIKpnISacIBanIIBceAIBsaBIEcoRIAcc65IEco53kIG170GL172SS174VI176AQ178WF180RV182KM184TD186RI188FG190LY192YA194EL196TH198NK200K*2021069-1674bzcsx27pUC191,5801,6001,6201,6401,6601,6801,7001,7201,740
    aatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttBglIFspIKasINarINdeIPvuISfoIPluTIPvuIIBstAPIpUC191,7601,7801,8001,8201,8401,8601,8801,9001,920
    tctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccDrdIPfoIBsmBIBsmBIEsp3IEsp3IEcoO109IpUC19pUC191,9401,9601,9802,0002,0202,0402,0602,0802,100
    cctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatatggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataSspIZraIAatIIBciVIpUC192,1202,1402,1602,1802,2002,2202,2402,260
    tgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagaactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctAcuIblapUC192,2802,3002,3202,3402,3602,3802,4002,4202,440
    tccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgAclIBcgIScaIXmnIblaNew AGAAW target 2pUC192,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcccctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggPvuIAvaIIblapUC192,6402,6602,6802,7002,7202,7402,7602,7802,800
    ataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttatatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaatAclIBglIFspIAvaIINmeAIIIblapUC192,8202,8402,8602,8802,9002,9202,9402,960
    ttgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaaacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccatAhdIBmrIBpmIBsaIBsrFIblapUC192,9803,0003,0203,0403,0603,0803,1003,1203,140
    actgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgatgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactpUC193,1603,1803,2003,2203,2403,2603,2803,3003,320
    gatccttttttctaggaaaaaapUC19pM...n3,330
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    Editing disabled on read-only sequence.
    3336 bp250027503000250500750pUC19blaRNAI promoter (clockwise); TTGAAGpMB1 origin of replication

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    3336