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U6.3 SaCas9_cloning
  • Sequence Map
    Editing disabled on read-only sequence.
    attgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttttaacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaaSspIBciVIBspHICspCI20406080100120140160
    aaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctttggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggaAloIDrdIPsiIBsaAIBsaXIBtgZIDraIIIF1 ori180200220240260280300320340
    aatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacttagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattgNaeINgoMIVF1 ori360380400420440460480500520
    caccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcagtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtBglIPvuIBsmFILacZ alphaF1 ori540560580600620640660680700
    cgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagctccaccgcggtggcggccgctctagaactagtggatcccccgggctgcCGGCACACCCTTACTTTCAGGTTCAGGGCACGACCAGCAAGCACCTCTCTCGTTTCCTCCTCAGCAgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacctcgaggtggcgccaccgccggcgagatcttgatcacctagggggcccgacgGCCGTGTGGGAATGAAAGTCCAAGTCCCGTGCTGGTCGTTCGTGGAGAGAGCAAAGGAGGAGTCGTAleIBcgIBtgIEagINaeINotISmaISpeIXbaIXmaIBamHIBbvCIBseRIBstXISacIITspMIBpu10INgoMIVPU6.3T7M13-fwdFragme...rev))720740760780800820840860
    GACAGCACCTTTTCCCGTGTAGCAGCACCAGTGCACCGTTGACCCCAATGGCATTTGGTCGTCTGAGCTCTGTTGGCGCAGCGGCAGGAATTGCAGGCACGCTGCAGGCTGGCCGTGCCAGACGTAAACATGCTACAGCACGCTGCGGTATTGTGGCGGCTATGTGTCGTGTGCCCTGTCGTGGAAAAGGGCACATCGTCGTGGTCACGTGGCAACTGGGGTTACCGTAAACCAGCAGACTCGAGACAACCGCGTCGCCGTCCTTAACGTCCGTGCGACGTCCGACCGGCACGGTCTGCATTTGTACGATGTCGTGCGACGCCATAACACCGCCGATACACAGCACACGGBstAPIPU6.38809009209409609801,0001,0201,040
    TGCAGTCCTATTACGTCATACTCCCAGTGCAGTGTACTACGACCACGACTACGACTACTTGCTGCATCACGCCCCCATCCGGTTGGTGCCACCCATCCCATACGCCCATACGCCACTTCATGTGACGGCTGTGGCTGGGGAGCTCCTTGGTGGCCGCTAAGCGGCCTCTGTGGTGACGTCAGGATAATGCAGTATGAGGGTCACGTCACATGATGCTGGTGCTGATGCTGATGAACGACGTAGTGCGGGGGTAGGCCAACCACGGTGGGTAGGGTATGCGGGTATGCGGTGAAGTACACTGCCGACACCGACCCCTCGAGGAACCACCGGCGATTCGCCGGAGACACCACBlpIBsgIStyIBstXIPU6.31,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTGTGGAGCCTGCTCCCTGCTCCCTTGTAAGAAACCTAGTTCCAAAAGGGCATGCTCGTTACATCCCTGCTCCCTGCTCCCTTAGCAGCCGCGTAGCGGCGaCTGCTCCCTGCGCAGCTGTGGCTGTGTCCGCCAGGTGACCGCGGAGCGGCGGCTGCGGCCGCGAAGCGTAGTAGACACCTCGGACGAGGGACGAGGGAACATTCTTTGGATCAAGGTTTTCCCGTACGAGCAATGTAGGGACGAGGGACGAGGGAATCGTCGGCGCATCGCCGCtGACGAGGGACGCGTCGACACCGACACAGGCGGTCCACTGGCGCCTCGCCGCCGACGCCGGCGCTTCGCATCATBtgIEagINotISphISacIIBpu10IBstEIIPU6.31,2401,2601,2801,3001,3201,3401,3601,3801,400
    CCCCGTGCCTCCACTAACGCATGCACGGTATATATTTAACTGTTCTGTGTTTTATacttTGAGACGTACCATATGAACCGTCTCCgttttagtactctggaaacagaatctactaaaacaaggcaaaatgccgtgtttatctcgtcaacttgttggcgagattttttcccgggCTGGGGCACGGAGGTGATTGCGTACGTGCCATATATAAATTGACAAGACACAAAATAtgaaACTCTGCATGGTATACTTGGCAGAGGcaaaatcatgagacctttgtcttagatgattttgttccgttttacggcacaaatagagcagttgaacaaccgctctaaaaaagggcccGANdeIScaISmaISphIXmaIBsmBIBsmBIEsp3IEsp3ITspMISaCas9 ScaffoldPU6.3Cloning Insert1,4201,4401,4601,4801,5001,5201,5401,560
    GCAGGAATTCGAtatcaagcttatcgataccgtcgacctcgagggggggcccggtacccagcttttgttccctttagtgagggttaattGCGCgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagCGTCCTTAAGCTatagttcgaatagctatggcagctggagctcccccccgggccatgggtcgaaaacaagggaaatcactcccaattaaCGCGcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcAccIApaIClaIKpnISalITliIXhoIBspDIEcoRIEcoRVPspXIAcc65IBssHIIPaeR7IPspOMIHindIIIEcoO109ILacOM13-revT3T3 promoterlac promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgaSapIBsaXIBspQIlac...er1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccctgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggPciIAflIIIColE1 origin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaDrdIBciVIBssSIBssSαIColE1 origin2,1202,1402,1602,1802,2002,2202,2402,260
    ttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgAlwNIColE1 origin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccacAcuIColE1 origin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagcaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcBspHIColE1 origin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgtAhdIAmpR2,8202,8402,8602,8802,9002,9202,9402,960
    atgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgtactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacAclIBglIBpmIBsaIAvaIIBsrDINmeAIIIAmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgaPvuIAvaIIBsrDIAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
    catggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttBcgIScaIBsaHIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaacacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttAclIAcuIXmnIBssSIBssSαI3,5203,5403,5603,5803,6003,6203,6403,660
    taagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataaSspI3,6803,7003,720
    • Plasmid
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    Editing disabled on read-only sequence.
    3726 bp2750300032503500250500750PU6.3AmpRpBluKSP_SaCas9\Scaffold\cloning REV (DNA)p045_PU6.3_(pJET,_841) FWD (DNA)BspHI,BciVI,SspI,CspCIPsiI,AloI,BsaXI,DrdIF1 ori,BsaAI,DraIII,BtgZINgoMIV,NaeIBsmFIBglILacZ alpha,PvuIM13-fwd,T7+15BseRIBbvCIBpu10I,BcgIAhdIBsaI,BsrDI,BpmIBglI,AvaII,NmeAIIIAclIBsrDIAvaIIPvuIScaI,BcgI,BsaHI,AclI,XmnIBssSI,BssSαI,AcuI,SspI

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    3726