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pAAV_hSyn FLEX-hM4Di-mCitrine-2ACT (JGE204)
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagactgcagagggcggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcacacagatctgacgtctcccgEagIMluINotISbfIAflIIIL-ITRHuman S...omoter20406080100120140160
    cctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccggacgcatactcacgttcacccaaaatcctggtcctactccgccccacccccacggatggactgctggctggggctgggtgacctgttcgtgggttgggggtaaggggtttaacgcgtaggggatagtctctccccctcccctttgtcctacgccgctccgcgcacgcgtgacggFspAIHuman Synapsin promoter180200220240260280300320340
    agcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgctcgaagtcgtggcgcctgtcacggaagcgggggcggaccgccgcgcgcggtggcggcggagtcgtgacttccgcgcgactgcagtgagcggccagggggcgtttgaggggaagggccggtggaaccagcgcaggcgcggcggcggccgggtcggcctggcgtggtgcgctccgcgBbvCIRsrIIHuman Synapsin promoter360380400420440460480500520
    gagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgagaaggtaccggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagctctatccccccgtgcccgcgctggtagacgcgacgccgcggccgctgagtcgcgacggagtcagacgccacccgtcgcctcctcagcacagcacggactctcgcgtcagctcttccatggcctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcAccIKpnIMreISalIBamHIBspEISgrAIAcc65IHuman Synapsin promoterlox2722AAVseq F540560580600620640660680700
    ttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgcctcacacacagctaaccttttcattcacggtttcaatattgtctgtacaattctcttccgactgttgtttccccagtgtaaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcggagtgtgtgtcgattggaaaagtaagtgccaaagttataacagacatgttaagagaaggctgacaacaaaggggtcacatAscI*330VC328SV326KE324NV322TE320IN318DT316CN314EE312SQ310QK308GL306T797-17862ACTloxP720740760780800820840860
    accatggagcagtcatcaactgtctgctcagcatcttcctgtgagttctttttctgtcccacctggagctgactagacttgtaggccaatgctggtatagtgttcactaaaattaactgcagtggctttctgttttccttgtactgacactgaatgtaccttgagaaggcggacctggtacctcgtcagtagttgacagacgagtcgtagaaggacactcaagaaaaagacagggtggacctcgactgatctgaacatccggttacgaccatatcacaagtgattttaattgacgtcaccgaaagacaaaaggaacatgactgtgacttacatggaactcttccgcctggBlpINcoIEcoNIV304MS302CD300DV298TQ296EA294DE292QS290NK288KQ286GV284QL282QS280SK278YA276LA274PI272TN270VL268IL266QL264PK262RN260EK258YQ256CQ254IY252RS250FA248SR246797-17862ACT8809009209409609801,0001,0201,040
    tataagttttggcgcgtcccttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagtttggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggatattcaaaaccgcgcagggaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacgagtcaaacctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccYT244KA242RG240KY238LE236DM234GL232TI230GA228AT226VF224EL222LV220MH218DR216KE214NP212DK210SL208KS206QY204SL202YH200ND198PL196LV194PG192DG190IP188797-1786mCitrine2ACT1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgccacaagacgaccatcaccagccgctcgacgtgcgacggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctgcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagcTN186QQ184YH182DA180LQ178VS176GD174EI172NH170RI168KF166NV164KI162GN160KQ158KD156AM154IY152VN150HS148NY146NY144EL142KH140GL138IN136GD134EK132FD130797-1786mCitrine1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagctacgggaagtcgagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgIG128KL126EI124RN122VL120TD118GE116FK114VE112AR110TK108YN106GD104DK102FF100IT98RE96QV94YG92EP90MA88SK86FF84DH82QK80MH78DP76YR74AF72C797-1786mCitrine1,4201,4401,4601,4801,5001,5201,5401,560
    acatcaggccgtagccgaaggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcactgtagtccggcatcggcttccaccagtgctcccacccggtcccgtgcccgtcgaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtgM70LG68YG66FT64TV62LT60PW58PV56PL54KG52TT50CI48FK46LT44LK42GY40TA38DG36EG34EG32SV30SF28KH26GN24VD22GD20LE18VL16IP14V797-1786mCitrine1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caccccggtgaacagctcctcgcccttgctcaccataccggtcctggcagtgccgatgttccgatactggcacagcagcaggtgccggaaggtctttttaaaggtggcgttgcacagagcatagcaggcagggttgatggtgctgttgacgtagcagagccagtagccaatggacgtggggccacttgtcgaggagcgggaacgagtggtatggccaggaccgtcacggctacaaggctatgaccgtgtcgtcgtccacggccttccagaaaaatttccaccgcaacgtgtctcgtatcgtccgtcccaactaccacgacaactgcatcgtctcggtcatcggttacctgAgeIBfuAIBspMIPaqCIBstAPIV12GT10FL8EE6GK4SV2M797-1786hM4DimCitrine1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cacaccgtgtcagggatgcagctctggcagaaggtgttcaccaggaccatgacgttgtagggcgtccaggtgaggatgaaggctagtagaatggcaaagatcgttcgtgtcactttgcgctcccgggccgccatctgccgcttcttgcgcacctggttgcgagcgatgctggcgagtgtggcacagtccctacgtcgagaccgtcttccacaagtggtcctggtactgcaacatcccgcaggtccactcctacttccgatcatcttaccgtttctagcaagcacagtgaaacgcgagggcccggcggtagacggcgaagaacgcgtggaccaacgctcgctacgaccgctSexAIDraIIIhM4Di1,9401,9601,9802,0002,0202,0402,0602,0802,100
    acttgcgggccacgttggccgcagggcgcatgccagccggcgtggcaggcacaatctcaatggctgtcacacactcattgcctgtctgcttcgtcacaatctggatcttggaccatctggaggctgggttgagggcccgcggctgcaggggaggggcgggcatggcgggcgtggttgaacgcccggtgcaaccggcgtcccgcgtacggtcggccgcaccgtccgtgttagagttaccgacagtgtgtgagtaacggacagacgaagcagtgttagacctagaacctggtagacctccgacccaactcccgggcgccgacgtcccctccccgcccgtaccgcccgcaccaSfiISphIhM4Di2,1202,1402,1602,1802,2002,2202,2402,260
    ggcctctgtggtggacagctctgtggctgggcgttccttggtgttctgggtggcactgcctgagctggactcattggaagtgtccttatcagccacggggcgcggtggcggtggcagcgctggcgggggggcctcctccagcttgccattgcgcagctcctcccgggcggcctccccggagacaccacctgtcgagacaccgacccgcaaggaaccacaagacccaccgtgacggactcgacctgagtaaccttcacaggaatagtcggtgccccgcgccaccgccaccgtcgcgaccgcccccccggaggaggtcgaacggtaacgcgtcgaggagggcccgccggagghM4Di2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ccgggcgggggcttcttgacgctctgcttcattagtgggctcttgaggaaggccagcgtcttggctttcttctccttcgggccctcgggccggtgcttgtggactcggctgcgactggccagggagatgtggatgtacagcaccgtcatgatgaccacaggcaggtagaagccagggcccgcccccgaagaactgcgagacgaagtaatcacccgagaactccttccggtcgcagaaccgaaagaagaggaagcccgggagcccggccacgaacacctgagccgacgctgaccggtccctctacacctacatgtcgtggcagtactactggtgtccgtccatcttcggtcBfuAIBspMIhM4Di2,4602,4802,5002,5202,5402,5602,5802,6002,620
    caatggctgtgccaaaggtcactgctgggttggacaggaactggatgaagcactggttgtcgggcaccgtccgcttacccaccacaaactgccagaacaagatggcaggcgcccagagcacgaaggacagtacccaggcagcagcaatcatgaggcctgccatcttggtggtgcggttaccgacacggtttccagtgacgacccaacctgtccttgacctacttcgtgaccaacagcccgtggcaggcgaatgggtggtgtttgacggtcttgttctaccgtccgcgggtctcgtgcttcctgtcatgggtccgtcgtcgttagtactccggacggtagaaccaccacgcStuIAlwNIhM4Di2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccgggcagggtaggtgagaggcttggtgacgcagaagtagcggtcaaagctgatgatgagaaggttcatgacggaggcgttgctcaccacgcagtccagggccagccacaggtcgcagaccacggcgcccaggggccagtagcccttgatgatgtacacggtgtagaggttcatgggcccgtcccatccactctccgaaccactgcgtcttcatcgccagtttcgactactactcttccaagtactgcctccgcaacgagtggtgcgtcaggtcccggtcggtgtccagcgtctggtgccgcgggtccccggtcatcgggaactactacatgtgccacatctccaagtacPasIhM4Di2,8202,8402,8602,8802,9002,9202,9402,960
    gagaaggcgcctatgatgagatcagcacacgccaggctgaagaggaagtagttgttgactgtctgcagctgcctgttgaccttgatggacagcatcaccaggatgttgcccacgacagtcaccaggctcagggagcctgtcactgtggcaatgaagaccatttccaccgtctcatctcttccgcggatactactctagtcgtgtgcggtccgacttctccttcatcaacaactgacagacgtcgacggacaactggaactacctgtcgtagtggtcctacaacgggtgctgtcagtggtccgagtccctcggacagtgacaccgttacttctggtaaaggtggcagagtahM4Di2,9803,0003,0203,0403,0603,0803,1003,1203,140
    agcgattgtgggatgatgacgtgaccaggcgcacggactgattgcccgagctgccattgacaggtgtgaagttggccatagcgtaatctggaacatcgtatgggtacatggtggctctagagggcccgtttaaacgctagcataacttcgtataaagtatcctatacgaagttattcgctaacaccctactactgcactggtccgcgtgcctgactaacgggctcgacggtaactgtccacacttcaaccggtatcgcattagaccttgtagcatacccatgtaccaccgagatctcccgggcaaatttgcgatcgtattgaagcatatttcataggatatgcttcaataBmtINheIPmeIBmgBIhM4Dilox27223,1603,1803,2003,2203,2403,2603,2803,3003,320
    ttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttaaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaatacttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatClaIBspDIEcoRIEcoRVHindIIIWPREloxP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactgggcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccWPREAAVseq R3,5203,5403,5603,5803,6003,6203,6403,660
    ttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacPflMIWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagClaIBspDIWPRE3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggagggggctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctcccccTliIXhoIBglIICspCIPaeR7IhGH polyA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgacaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactNsiISphIBbvCIhGH polyA4,2204,2404,2604,2804,3004,3204,3404,360
    ccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgAloIBlpIBsaIBstEIIhGH polyA4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccaEagINotIPmlISbfIBsaAIRsrIIR-ITR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    attttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctctaaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaBsaAIDraIIIf1 Origin4,9204,9404,9604,9805,0005,0205,0405,060
    ttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcacaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgAloIPsiIf1 Origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgc5,2605,2805,3005,3205,3405,3605,3805,4005,420
    agacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacg5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaaaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    cagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaagtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttScaIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    aagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagcttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgPvuIAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggcacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgAseIAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaaBsaIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    aagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaagttcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcpUC oriAmpR6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaapUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    aggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccctccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggAlwNIpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    agcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacpUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    tcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori7,1807,2007,2207,2407,2607,2807,300
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    7307 bp5000550060006500700050010001500hM4DiAAVseq F797-1786

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    7307