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  • 0 feature results
pBzCas13b
  • Sequence Map
    Editing disabled on read-only sequence.
    GAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGCCTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTTGCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTACGGTABspEIEcoRIPvuII20406080100120140160
    TGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGAATCGAGGACTTTTAGAGCTATTGAGTTTTTTATGCGGGCCATCACTAGAATAAAGTAATACCACTTTCAACCTTGGAGAATGCACGGCTAGTTGCAGAGTAAAAGCGGTTTTCAACCGGGPasIBsaAIBsmBIEsp3I180200220240260280300320340
    AGGGCTTCCCGGTATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTTCCCGAAGGGCCATAGTTGTCCCTGTGGTCCTAAATAAATAAGACGCTTCACTAGAAGGCAGTGTCCATAAATAAGCCGCGTTTCACGCAGCCCACTACGACGGTTGAATGACTAAATCACATACTACCACAAAAACTCCACGAGGTCACCGAAGACAAAGATAGTCGACAGGGAPvuII360380400420440460480500520
    CCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACAGGAGGACAAGTCGATGACTGCCCCACCACGCATTGCCGTTTTCGTGGCGGCCTGTAGTCGCGATCGCCTCACATATGACCGAATGATACAACCGTGACTACTCCCACAGTCACTTCACGAAGTACACCGTCCTCTTTTTTCCGACGTGGCCACGCAGTCGTCTTATACACTATGTCCTAccISgrAIBstZ17I540560580600620640660680700
    TATATTCCGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGACAAGATATAAGGCGAAGGAGCGAGTGACTGAGCGATGCGAGCCAGCAAGCTGACGCCGCTCGCCTTTACCGAATGCTTGCCCCGCCTCTAAAGGACCTTCTACGGTCCTTCTATGAATTGTCCCTTCACTCTCCCGGCGCCGTTTCGGCAAAAAGGTATCCGAGGCGGGGGGACTGTTCPfoISacII720740760780800820840860
    CATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTGTAGTGCTTTAGACTGCGAGTTTAGTCACCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCGCCGAGGGAGCACGCGAGAGGACAAGGACGGAAAGCCAAATGGCCACAGTAAGGCGACAATACCGGCGCAAACAGAGTAAGGTGCGGACTGTGAGTCABssSIBssSαI8809009209409609801,0001,0201,040
    TCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCAGGCCCATCCGTCAAGCGAGGTTCGACCTGACATACGTGCTTGGGGGGCAAGTCAGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCTTTCTGTACGTTTTCGTGGTGACCGTCGTCGGTGACCATTAACTAAATCTCCTCAATCAGAACTTCAGTACGNspI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GCCGGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGATCTCAAGAAGATCCGGCCAATTCCGATTTGACTTTCCTGTTCAAAACCACTGACGCGAGGAGGTTCGGTCAATGGAGCCAAGTTTCTCAACCATCGAGTCTCTTGGAAGCTTTTTGGCGGGACGTTCCGCCAAAAAAGCAAAAGTCTCGTTCTCTAATGCGCGTCTGGTTTTGCTAGAGTTCTTCTAG1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ATCTTATTAATCAGATAAAATATTTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATATAAGTTGTAATTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACTAGAATAATTAGTCTATTTTATAAAGATCTAAAGTCACGTTAAATAGAGAAGTTTACATCGTGGACTTCAGTCGGGGTATGCTATATTCAACATTAAGAGTACAAACTGTCGAATAGTAGCTATTCGAAATTACGCCATCAAATAGTGTCAATTTAACGATTGCGTCAGTCCGTGAseIXbaI1,4201,4401,4601,4801,5001,5201,5401,560
    CGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATTTTACACTTTATGCTTCCGGCTCGTATGTTAGGAGGTCTTTATCATGGAAAACAAAACATCTTTGGGGGCACATACTTTAGATTGTTACGCGAGTAGCAGTAGGAGCCGTGGCAGTGGGACCTACGACATCCGTATCCGAACCAATACGGCCATGACGGCCCGGAGAACGCCCTAAAATGTGAAATACGAAGGCCGAGCATACAATCCTCCAGAAATAGTACCTTTTGTTTTGTAGAAACCCCSfcIME2NK4TS6LG81727-5398Gibson homol...EcoRV-pACYCLac promoterbzcas13b1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AATAATATTTATTACAACCCTTTTAAACCACAAGATAAATCGTATTTTGCGGGTTATTTCAATGCAGCAATGGAAAATACAGATAGTGTATTTAGAGAATTGGGAAAAAGATTAAAAGGTAAAGAGTACACTTCGGAGAATTTTTTTGATGCTATCTTTAAGGAAAATATTTCATTTATTATAAATAATGTTGGGAAAATTTGGTGTTCTATTTAGCATAAAACGCCCAATAAAGTTACGTCGTTACCTTTTATGTCTATCACATAAATCTCTTAACCCTTTTTCTAATTTTCCATTTCTCATGTGAAGCCTCTTAAAAAAACTACGATAGAAATTCCTTTTATAAAGTANN10IY12YN14PF16KP18QD20KS22YF24AG26YF28NA30AM32EN34TD36SV38FR40EL42GK44RL46KG48KE50YT52SE54NF56FD58AI60FK62EN64IS661727-5398bzcas13b1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TAGTGGAATACGAAAGGTATGTGAAATTGCTTTCTGATTATTTCCCTATGGCTCGTCTTTTGGATAAAAAAGAAGTTCCTATAAAAGAACGAAAAGAAAATTTTAAAAAGAATTTTAAAGGCATTATAAAAGCCGTAAGAGATTTAAGAAACTTTTATACGCATAAGGAACACGGATCACCTTATGCTTTCCATACACTTTAACGAAAGACTAATAAAGGGATACCGAGCAGAAAACCTATTTTTTCTTCAAGGATATTTTCTTGCTTTTCTTTTAAAATTTTTCTTAAAATTTCCGTAATATTTTCGGCATTCTCTAAATTCTTTGAAAATATGCGTATTCCTTGTGCCLV68EY70ER72YV74KL76LS78DY80FP82MA84RL86LD88KK90EV92PI94KE96RK98EN100FK102KN104FK106GI108IK110AV112RD114LR116NF118YT120HK122EH124G1727-5398bzcas13b1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AGAGGTAGAAATTACAGATGAAATATTTGGCGTATTGGATGAGATGCTGAAAAGCACGGTTTTAACGGTAAAAAAGAAAAAAGTAAAAACCGATAAAACAAAAGAAATCCTGAAAAAAAGCATAGAAAAACAATTGGATATTTTATGCCAAAAGAAATTAGAATATCTAAGAGACTCTCCATCTTTAATGTCTACTTTATAAACCGCATAACCTACTCTACGACTTTTCGTGCCAAAATTGCCATTTTTTCTTTTTTCATTTTTGGCTATTTTGTTTTCTTTAGGACTTTTTTTCGTATCTTTTTGTTAACCTATAAAATACGGTTTTCTTTAATCTTATAGATTCTCTGMfeIE126VE128IT130DE132IF134GV136LD138EM140LK142ST144VL146TV148KK150KK152VK154TD156KT158KE160IL162KK164SI166EK168QL170DI172LC174QK176KL178EY180LR182D1727-5398bzcas13b2,1202,1402,1602,1802,2002,2202,2402,260
    ACAGCAAGAAAAATAGAGGAGAAGCGTAGAAATCAAAGAGAAAGAGGAGAGAAAGAATTGGTAGCTCCATTCAAATACAGTGATAAAAGGGATGATTTAATAGCAGCGATTTATAATGATGCTTTTGATGTTTATATCGATAAGAAAAAAGATAGCCTAAAAGAATCCAGCAAAGTGTCGTTCTTTTTATCTCCTCTTCGCATCTTTAGTTTCTCTTTCTCCTCTCTTTCTTAACCATCGAGGTAAGTTTATGTCACTATTTTCCCTACTAAATTATCGTCGCTAAATATTACTACGAAAACTACAAATATAGCTATTCTTTTTTCTATCGGATTTTCTTAGGTCGTTTCT184AR186KI188EE190KR192RN194QR196ER198GE200KE202LV204AP206FK208YS210DK212RD214DL216IA218AI220YN222DA224FD226VY228ID230KK232KD234SL236KE238SS240K1727-5398bzcas13b2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CAAAGTACAATACCAAAAGTGATCCTCAGCAAGAAGAAGGCGATTTGAAAATTCCAATTTCGAAAAACGGAGTAGTCTTCCTGCTTTCTTTGTTTCTCACCAAACAAGAAATACACGCATTCAAATCTAAAATAGCAGGGTTTAAGGCTACGGTTATCGATGAAGCAACGGTATCGTTTCATGTTATGGTTTTCACTAGGAGTCGTTCTTCTTCCGCTAAACTTTTAAGGTTAAAGCTTTTTGCCTCATCAGAAGGACGAAAGAAACAAAGAGTGGTTTGTTCTTTATGTGCGTAAGTTTAGATTTTATCGTCCCAAATTCCGATGCCAATAGCTACTTCGTTGCCATAGBbvCIA242KY244NT246KS248DP250QQ252EE254GD256LK258IP260IS262KN264GV266VF268LL270SL272FL274TK276QE278IH280AF282KS284KI286AG288FK290AT292VI294DE296AT298VS3001727-5398bzcas13b2,4602,4802,5002,5202,5402,5602,5802,6002,620
    TGAAGCAACGGTGAGCCATGGAAAAAACAGCATTTGTTTTATGGCTACGCACGAAATTTTCTCGCATTTGGCTTACAAAAAATTGAAACGAAAAGTGAGAACGGCTGAAATCAACTATGGTGAGGCAGAAAATGCTGAACAGCTTTCCGTTTATGCCAAAGAAACTCTAATGATGACTTCGTTGCCACTCGGTACCTTTTTTGTCGTAAACAAAATACCGATGCGTGCTTTAAAAGAGCGTAAACCGAATGTTTTTTAACTTTGCTTTTCACTCTTGCCGACTTTAGTTGATACCACTCCGTCTTTTACGACTTGTCGAAAGGCAAATACGGTTTCTTTGAGATTACTACBstAPIEA302TV304SH306GK308NS310IC312FM314AT316HE318IF320SH322LA324YK326KL328KR330KV332RT334AE336IN338YG340EA342EN344AE346QL348SV350YA352KE354TL356MM3581727-5398bzcas13b2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CAGATGCTTGATGAATTGAGCAAAGTACCCGATGTGGTCTATCAGAATTTGAGCGAAGATGTACAAAAAACTTTCATCGAAGATTGGAATGAATACCTAAAAGAGAACAATGGCGATGTAGGGACAATGGAAGAAGAGCAGGTAATTCATCCAGTGATTCGCAAGCGTTACGAAGGTCTACGAACTACTTAACTCGTTTCATGGGCTACACCAGATAGTCTTAAACTCGCTTCTACATGTTTTTTGAAAGTAGCTTCTAACCTTACTTATGGATTTTCTCTTGTTACCGCTACATCCCTGTTACCTTCTTCTCGTCCATTAAGTAGGTCACTAAGCGTTCGCAATGCTTCSapIBfuAIBspMIBspQIBsrGIQM360LD362EL364SK366VP368DV370VY372QN374LS376ED378VQ380KT382FI384ED386WN388EY390LK392EN394NG396DV398GT400ME402EE404QV406IH408PV410IR412KR414YE4161727-5398bzcas13b2,8202,8402,8602,8802,9002,9202,9402,960
    ATAAATTCAATTATTTTGCCATTCGATTTTTAGATGAGTTTGCTCAGTTCCCTACGCTTCGTTTTCAAGTGCATTTAGGGAATTATCTTCACGATTCTCGCCCTAAAGAAAATCTGATTTCTGACCGAAGAATAAAAGAAAAAATCACCGTTTTCGGAAGGCTTTCGGAATTGGATATTTAAGTTAATAAAACGGTAAGCTAAAAATCTACTCAAACGAGTCAAGGGATGCGAAGCAAAAGTTCACGTAAATCCCTTAATAGAAGTGCTAAGAGCGGGATTTCTTTTAGACTAAAGACTGGCTTCTTATTTTCTTTTTTAGTGGCAAAAGCCTTCCGAAAGCCTTAACCTDK418FN420YF422AI424RF426LD428EF430AQ432FP434TL436RF438QV440HL442GN444YL446HD448SR450PK452EN454LI456SD458RR460IK462EK464IT466VF468GR470LS472EL474E1727-5398bzcas13b2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACATAAAAAAGCATTGTTTATAAAAAATACCGAAACCAATGAAGATAGAGAACACTATTGGGAAATTTTTCCTAACCCCAATTATGATTTTCCTAAGGAAAATATTTCCGTTAATGATAAAGATTTTCCTATCGCGGGTAGTATTTTGGACAGAGAGAAACAGCCTGTCGCAGGGTGTATTTTTTCGTAACAAATATTTTTTATGGCTTTGGTTACTTCTATCTCTTGTGATAACCCTTTAAAAAGGATTGGGGTTAATACTAAAAGGATTCCTTTTATAAAGGCAATTACTATTTCTAAAAGGATAGCGCCCATCATAAAACCTGTCTCTCTTTGTCGGACAGCGTCCCBsu36IH476KK478AL480FI482KN484TE486TN488ED490RE492HY494WE496IF498PN500PN502YD504FP506KE508NI510SV512ND514KD516FP518IA520GS522IL524DR526EK528QP530VA532G1727-5398bzcas13b3,1603,1803,2003,2203,2403,2603,2803,3003,320
    AAAATTGGTATAAAAGTGAAATTGCTTAACCAACAATATGTTTCAGAAGTAGATAAAGCCGTAAAAGCCCATCAGCTCAAGCAAAGAAAAGCCAGCAAGCCGTCTATCCAGAACATTATAGAAGAAATAGTGCCAATCAATGAAAGCAATCCAAAAGAAGCTATTGTTTTCGGTGTTTTAACCATATTTTCACTTTAACGAATTGGTTGTTATACAAAGTCTTCATCTATTTCGGCATTTTCGGGTAGTCGAGTTCGTTTCTTTTCGGTCGTTCGGCAGATAGGTCTTGTAATATCTTCTTTATCACGGTTAGTTACTTTCGTTAGGTTTTCTTCGATAACAAAAGCCACK534IG536IK538VK540LL542NQ544QY546VS548EV550DK552AV554KA556HQ558LK560QR562KA564SK566PS568IQ570NI572IE574EI576VP578IN580ES582NP584KE586AI588VF590G1727-5398bzcas13b3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GGCAACCCACGGCTTATCTGAGTATGAATGACATTCATTCCATTTTGTATGAGTTTTTCGATAAATGGGAGAAAAAGAAAGAAAAGTTAGAAAAGAAAGGTGAAAAAGAATTAAGAAAGGAAATTGGAAAAGAGTTAGAAAAGAAAATCGTAGGAAAAATTCAAGCTCAAATCCACCGTTGGGTGCCGAATAGACTCATACTTACTGTAAGTAAGGTAAAACATACTCAAAAAGCTATTTACCCTCTTTTTCTTTCTTTTCAATCTTTTCTTTCCACTTTTTCTTAATTCTTTCCTTTAACCTTTTCTCAATCTTTTCTTTTAGCATCCTTTTTAAGTTCGAGTTTAGGTG592QP594TA596YL598SM600ND602IH604SI606LY608EF610FD612KW614EK616KK618EK620LE622KK624GE626KE628LR630KE632IG634KE636LE638KK640IV642GK644IQ646AQ648IQ6501727-5398bzcas13b3,5203,5403,5603,5803,6003,6203,6403,660
    GCAAATCATAGATAAAGATACCAATGCCAAGATATTAAAACCTTATCAAGACGGAAATTCTACTGCTATTGATAAAGAGAAATTGATAAAAGATTTGAAGCAAGAGCAAAACATTCTACAAAAATTAAAAGATGAACAAACGGTTCGTGAAAAAGAATATAATGATTTTATTGCTCGTTTAGTATCTATTTCTATGGTTACGGTTCTATAATTTTGGAATAGTTCTGCCTTTAAGATGACGATAACTATTTCTCTTTAACTATTTTCTAAACTTCGTTCTCGTTTTGTAAGATGTTTTTAATTTTCTACTTGTTTGCCAAGCACTTTTTCTTATATTACTAAAATAACGAQI652ID654KD656TN658AK660IL662KP664YQ666DG668NS670TA672ID674KE676KL678IK680DL682KQ684EQ686NI688LQ690KL692KD694EQ696TV698RE700KE702YN704DF706IA7081727-5398bzcas13b3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TATCAAGATAAAAATAGGGAGATAAATAAAGTCAGAGATAGAAATCATAAACAATATTTAAAAGATAACCTTAAACGAAAGTACCCTGAAGCTCCTGCACGAAAAGAAGTTCTTTATTATCGAGAAAAAGGAAAAGTAGCGGTTTGGTTGGCGAATGACATCAAACGCTTTATGCATAGTTCTATTTTTATCCCTCTATTTATTTCAGTCTCTATCTTTAGTATTTGTTATAAATTTTCTATTGGAATTTGCTTTCATGGGACTTCGAGGACGTGCTTTTCTTCAAGAAATAATAGCTCTTTTTCCTTTTCATCGCCAAACCAACCGCTTACTGTAGTTTGCGAAATACGBaeIYQ710DK712NR714EI716NK718VR720DR722NH724KQ726YL728KD730NL732KR734KY736PE738AP740AR742KE744VL746YY748RE750KG752KV754AV756WL758AN760DI762KR764FM7661727-5398bzcas13b3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CTACCGATTTCAAAAACGAATGGAAAGGGGAGCAACACAGCTTGTTACAAAAATCGTTGGCGTATTATGAGCAATGTAAAGAGGAGTTGAAAAATCTTTTGCCTGAAAAAGTATTTCAGCATTTACCTTTTAAGTTGGGAGGATATTTTCAACAAAAATATTTGTACCAATTTTAGATGGCTAAAGTTTTTGCTTACCTTTCCCCTCGTTGTGTCGAACAATGTTTTTAGCAACCGCATAATACTCGTTACATTTCTCCTCAACTTTTTAGAAAACGGACTTTTTCATAAAGTCGTAAATGGAAAATTCAACCCTCCTATAAAAGTTGTTTTTATAAACATGGTTAAAATPT768DF770KN772EW774KG776EQ778HS780LL782QK784SL786AY788YE790QC792KE794EL796KN798LL800PE802KV804FQ806HL808PF810KL812GG814YF816QQ818KY820LY822QF824Y1727-5398bzcas13b4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CACTTGTTATTTAGATAAACGGTTGGAATACATCAGTGGGTTGGTGCAACAAGCCGAAAACTTTAAATCCGAAAATAAAGTCTTCAAAAAGGTAGAAAATGAGTGTTTTAAATTTTTGAAAAAACAAAATTATACTCACAAAGAATTAGATGCTCGGGTTCAGTCTATATTGGGGGTGAACAATAAATCTATTTGCCAACCTTATGTAGTCACCCAACCACGTTGTTCGGCTTTTGAAATTTAGGCTTTTATTTCAGAAGTTTTTCCATCTTTTACTCACAAAATTTAAAAACTTTTTTGTTTTAATATGAGTGTTTCTTAATCTACGAGCCCAAGTCAGATATAACCCCT826CY828LD830KR832LE834YI836SG838LV840QQ842AE844NF846KS848EN850KV852FK854KV856EN858EC860FK862FL864KK866QN868YT870HK872EL874DA876RV878QS880IL882G1727-5398bzcas13b4,2204,2404,2604,2804,3004,3204,3404,360
    TATCCTATCTTTTTAGAGCGGGGCTTTATGGATGAAAAACCTACGATAATCAAAGGAAAAACTTTTAAAGGAAATGAAGCTCTTTTTGCCGATTGGTTTAGGTATTACAAGGAATATCAGAATTTTCAGACATTTTATGATACTGAAAATTATCCGCTTGTAGAATTGGAAAAAAATAGGATAGAAAAATCTCGCCCCGAAATACCTACTTTTTGGATGCTATTAGTTTCCTTTTTGAAAATTTCCTTTACTTCGAGAAAAACGGCTAACCAAATCCATAATGTTCCTTATAGTCTTAAAAGTCTGTAAAATACTATGACTTTTAATAGGCGAACATCTTAACCTTTTTTBciVIY884PI886FL888ER890GF892MD894EK896PT898II900KG902KT904FK906GN908EA910LF912AD914WF916RY918YK920EY922QN924FQ926TF928YD930TE932NY934PL936VE938LE940K1727-5398bzcas13b4,3804,4004,4204,4404,4604,4804,5004,5204,540
    AACAAGCAGACCGAAAACGAAAAACTAAAATCTACCAACAAAAGAAAAACGATGTGTTTACACTTTTGATGGCAAAACATATTTTCAAATCGGTATTCAAGCAGGATAGCATCGATCAGTTTTCGTTAGAAGATTTGTATCAATCTCGGGAGGAACGCTTAGGAAATCAGGAGAGTTGTTCGTCTGGCTTTTGCTTTTTGATTTTAGATGGTTGTTTTCTTTTTGCTACACAAATGTGAAAACTACCGTTTTGTATAAAAGTTTAGCCATAAGTTCGTCCTATCGTAGCTAGTCAAAAGCAATCTTCTAAACATAGTTAGAGCCCTCCTTGCGAATCCTTTAGTCCTCTCK942QA944DR946KR948KT950KI952YQ954QK956KN958DV960FT962LL964MA966KH968IF970KS972VF974KQ976DS978ID980QF982SL984ED986LY988QS990RE992ER994LG996NQ998ER10001727-5398bzcas13b4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AGCCAGACAAACGGGCGAGCGAAATACTAACTATATTTGGAACAAAACCGTTGATTTAAAGTTGTGTGACGGAAAAATTACGGTAGAAAATGTAAAGCTAAAAAATGTAGGAGATTTCATAAAATATGAATACGACCAAAGGGTTCAAGCTTTCTTAAAATATGAGGAAAATATATCGGTCTGTTTGCCCGCTCGCTTTATGATTGATATAAACCTTGTTTTGGCAACTAAATTTCAACACACTGCCTTTTTAATGCCATCTTTTACATTTCGATTTTTTACATCCTCTAAAGTATTTTATACTTATGCTGGTTTCCCAAGTTCGAAAGAATTTTATACTCCTTTTATATAR1002QT1004GE1006RN1008TN1010YI1012WN1014KT1016VD1018LK1020LC1022DG1024KI1026TV1028EN1030VK1032LK1034NV1036GD1038FI1040KY1042EY1044DQ1046RV1048QA1050FL1052KY1054EE1056NI10581727-5398bzcas13b4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GAATGGCAGGCGTTTCTAATCAAAGAGAGTAAGGAAGAGGAAAATTATCCGTATGTGGTGGAAAGAGAAATAGAGCAATATGAAAAAGTGAGAAGGGAAGAACTGTTGAAGGAAGTCCACCTTATAGAGGAATATATTTTGGAAAAGGTAAAAGATAAGGAAATATTAAAAAAAGCTTACCGTCCGCAAAGATTAGTTTCTCTCATTCCTTCTCCTTTTAATAGGCATACACCACCTTTCTCTTTATCTCGTTATACTTTTTCACTCTTCCCTTCTTGACAACTTCCTTCAGGTGGAATATCTCCTTATATAAAACCTTTTCCATTTTCTATTCCTTTATAATTTTTTTCEW1060QA1062FL1064IK1066ES1068KE1070EE1072NY1074PY1076VV1078ER1080EI1082EQ1084YE1086KV1088RR1090EE1092LL1094KE1096VH1098LI1100EE1102YI1104LE1106KV1108KD1110KE1112IL1114KK11161727-5398bzcas13b4,9204,9404,9604,9805,0005,0205,0405,060
    GGGATAATCAAAATTTCAAATACTACATTCTCAACGGACTTTTAAAGCAACTTAAAAATGAAGATGTGGAGAGCTATAAGGTATTCAACTTAAATACTGAACCTGAAGATGTGAACATTAACCAACTAAAACAAGAAGCAACCGATTTAGAACAAAAAGCTTTTGTTTTAACCTACCCTATTAGTTTTAAAGTTTATGATGTAAGAGTTGCCTGAAAATTTCGTTGAATTTTTACTTCTACACCTCTCGATATTCCATAAGTTGAATTTATGACTTGGACTTCTACACTTGTAATTGGTTGATTTTGTTCTTCGTTGGCTAAATCTTGTTTTTCGAAAACAAAATTGGATGD1118NQ1120NF1122KY1124YI1126LN1128GL1130LK1132QL1134KN1136ED1138VE1140SY1142KV1144FN1146LN1148TE1150PE1152DV1154NI1156NQ1158LK1160QE1162AT1164DL1166EQ1168KA1170FV1172LT1174Y1727-5398bzcas13b5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TATTCGTAATAAATTTGCACACAACCAATTACCTAAAAAAGAATTTTGGGACTATTGTCAAGAAAAATACGGCAAAATAGAAAAAGAAAAAACCTATGCCGAGTATTTTGCTGAGGTATTTAAAAAGGAAAAAGAAGCACTGATAAAGTAATTGACAGCTAGCTCAGTCCTAGGTATAAGCATTATTTAAACGTGTGTTGGTTAATGGATTTTTTCTTAAAACCCTGATAACAGTTCTTTTTATGCCGTTTTATCTTTTTCTTTTTTGGATACGGCTCATAAAACGACTCCATAAATTTTTCCTTTTTCTTCGTGACTATTTCATTAACTGTCGATCGAGTCAGGATCCAAvrIIBbvCII1176RN1178KF1180AH1182NQ1184LP1186KK1188EF1190WD1192YC1194QE1196KY1198GK1200IE1202KE1204KT1206YA1208EY1210FA1212EV1214FK1216KE1218KE1220AL1222IK12241727-5398*1bzcas13bJ23119 promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ATAATGCTAGCGCTGAGTTGGAACTGCTCTCATTTTGGAGGGTAATCACAACGAGACCATGGCGGAAGGTCTCAGTTGGAACTGCTCTCATTTTGGAGGGTAATCACAACATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCATATTACGATCGCGACTCAACCTTGACGAGAGTAAAACCTCCCATTAGTGTTGCTCTGGTACCGCCTTCCAGAGTCAACCTTGACGAGAGTAAAACCTCCCATTAGTGTTGTAGCAGGTAAGGCTGTCGTAGCGGTCAGTGATACCGCACGACGATCGCGATATACGCAACTACGTBsaIBsaIBZ DRBZ DRGibson homol...EcoRV pACYCJ2...r5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGTAAAGATACGCGTGGGCAAGAGCCTCGTGACAGGCTGGCGAAACCGGCGGCGGGTCAGGACGAGCGAAGCGATGAACCTCGGTGATAGCTGATGCGCTAGTACCGCTGGTGTGGGCAGGACACCTAGGAGATGCGGCCTGCGTAGCACCGGCCGTAGTGGCCGCGGTGTCCACGCBamHIBstYIFspAISgrAI5,6205,6405,6605,6805,7005,7205,7405,760
    GTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCACAACGACCGCGGATATAGCGGCTGTAGTGGCTACCCCTTCTAGCCCGAGCGGTGAAGCCCGAGTACTCGCGAACAAAGCCGCACCCATACCACCGTCCGGGGCACCGGCCCCCTGACAACCCGCGGTAGAGGAACGTACGTGGTAAGGAACGCCGCCGCCACGAGTTGCCGGAGTNspISphIBanIIBanIIBspHI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCTGGATGATGACCCGACGAAGGATTACGTCCTCAGCGTATTCCCTCTCGCAGCTGGCTACGGGAACTCTCGGAAGTTGGGTCAGTCGAGGAAGGCCACCCGCGCCCCGTACTGATAGCAGCGGCGTGAATACTGACAGAAGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGAccISalIHincII5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGCGAGACCCAGTAAAAGCCGCTCCTGGCGAAAGCGACCTCGCGCTGCTACTAGCCGGACAGCGAACGCCATAAGCCTTAGAACGTGCGGGAGCGAGTTCGGAAGCAGTGACCAGGGCGGTGGTTTGCAAAGCCGCTCTTCGTCCGGTAATAGCGGCCGTACCGCCGGCTGCGCGACAclIBglIEagIBseYI6,1406,1606,1806,2006,2206,2406,2606,2806,300
    GGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCCGATGCAGAACGACCGCAAGCGCTGCGCTCCGACCTACCGGAAGGGGTAATACTAAGAAGAGCGAAGGCCGCCGTAGCCCTACGGGCGCAACGTCCGGTACGACAGGTCCGTCCATCTACTGCTGGTAGTCCCTGTCGAAGTTCCTAGCGAGCGCCGAGAATGGTCGGATTGAANruIBfuAIBspMIBstAPI6,3206,3406,3606,3806,4006,4206,4406,460
    CGATCATTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGCTAGTAACCTGGCGACTAGCAGTGCCGCTAAATACGGCGGAGCCGCTCGTGTACCTTGCCCAACCGTACCTAACATCCGCGGCGGGATATGGAACAGACGGAGGGGCGCAACGCAGCGCCACGTACCTCGGCCCGGTGGAGCTGGACTTACCTTCGGCCGCCGTGGAGCGATTGBglI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    GGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTCCTAAGTGGTGAGGTTCTTAACCTCGGTTAGTTAAGAACGCCTCTTGACACTTACGCGTTTGGTTGGGAACCGTCTTGTATAGGTAGCGCAGGCGGTAGAGGTCGTCGGCGTGCGCCGCGTAGAGCCCGTCGCAACCCAGGACCGGTGCCCACGCGTACTAGCACGAGGACAGCAMscIFspAIPpuMI6,6606,6806,7006,7206,7406,7606,7806,8006,820
    TGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCCCCTACGTGACTCCTGGGCCGATCCGACCGCCCCAACGGAATGACCAATCGTCTTACTTAGTGGCTATGCGCTCGCTTGCACTTCGCTGACGACGACGTTTTGCAGACGCTGGACTCGTTGTTGTACTTACCAGAAGCCAAAGGCACAAAGCATTTCAGACCTTTGCGCCTTCAGGGGATGCACBsaAIPpuMI6,8406,8606,8806,9006,9206,9406,9606,9807,000
    CTGCTGAAGTTGCCCGCAACAGAGAGTGGAACCAACCGGTGATACCACGATACTATGACTGAGAGTCAACGCCATGAGCGGCCTCATTTCTTATTCTGAGTTACAACAGTCCGCACCGCTGTCCGGTAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGGACGACTTCAACGGGCGTTGTCTCTCACCTTGGTTGGCCACTATGGTGCTATGATACTGACTCTCAGTTGCGGTACTCGCCGGAGTAAAGAATAAGACTCAATGTTGTCAGGCGTGGCGACAGGCCATCGAGGAAGGCCACCCGCGCCCCGTACTGATAGCAGCGGCGTGAATACAhdIHincII7,0207,0407,0607,0807,1007,1207,1407,160
    ACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAATGACAGAAGAAATAGTACGTTGAGCATCCTGTCCACGGCCGTCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGGGACGTGGTAATACAAGGCCTAGACGTAGCGTCCTACGACGACCGATGGGACACCTTGTGGATGTAGACATAATTBspEIBstYI7,1807,2007,2207,2407,2607,2807,3007,3207,340
    CGAAGCGCTAACCGTTTTTATCAGGCTCTGGGAGGCAGAATAAATGATCATATCGTCAATTATTACCTCCACGGGGAGAGCCTGAGCAAACTGGCCTCAGGCATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTGCTTCGCGATTGGCAAAAATAGTCCGAGACCCTCCGTCTTATTTACTAGTATAGCAGTTAATAATGGAGGTGCCCCTCTCGGACTCGTTTGACCGGAGTCCGTAAACTCTTCGTGTGCCAGTGTGACGAAGGCCATCAGTTATTTGGCCATTTGGTCGTTATCTGTATTCGCCGABclIBtsIBtsαIBsu36I7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ATTTAACGACCCTGCCCTGAACCGACGACCGGGTCGAATTTGCTTTCGAATTTCTGCCATTCATCCGCTTATTATCACTTATTCAGGCGTAGCACCAGGCGTTTAAGGGCACCAATAACTGCCTTAAAAAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATAAATTGCTGGGACGGGACTTGGCTGCTGGCCCAGCTTAAACGAAAGCTTAAAGACGGTAAGTAGGCGAATAATAGTGAATAAGTCCGCATCGTGGTCCGCAAATTCCCGTGGTTATTGACGGAATTTTTTTAATGCGGGGCGGGACGGTGAGTAGCGTCATGACAACATTAAGTDrdIScaIBaeGIPflFITth111I7,5407,5607,5807,6007,6207,6407,6607,6807,700
    TTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATAATTCGTAAGACGGCTGTACCTTCGGTAGTGTCTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATATTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTAGTTTTGACCACTTTGAGTGGGTCCCTAMscIPasI7,7207,7407,7607,7807,8007,8207,8407,860
    TGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGACCGACTCTGCTTTTTGTATAAGAGTTATTTGGGAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACATCTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGAGTACCTTTTGCCACATTGTTCCAclIBsmBIEsp3I7,8807,9007,9207,9407,9607,9808,0008,0208,040
    GTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGTAACGGTATGC8,0608,080
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8098 bp6000650070007500500100015002000bzcas13b1727-5398EcoRI,BspEI,PvuII,BsaAI,BsmBI,Esp3I,PasIPvuII,AccI,BstZ17I,SgrAI,PfoISacII,BssSI,BssSαINspIAseI,XbaISfcIGibson homology EcoRV-pACYCLac promoterShine-Dalgarno sequenceAclI,BglI,EagI,BseYI,NruIBstAPIBfuAI,BspMI,BglIPpuMI,MscI,FspAI,PpuMIBsaAI,AhdI,HincIIBspEI,BstYIBclI,Bsu36I,BtsI,BtsαI,DrdI,PflFI,Tth111IBaeGI,ScaI,MscI,PasI,BsmBI,Esp3I,AclI

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    8098