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pR*G
  • Sequence Map
    Editing disabled on read-only sequence.
    ggccacgggtgcgcatgatctagagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatccggtgcccacgcgtactagatctcgacggagcgcgcaaagccactactgccacttttggagactgtgtacgtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcagtcccgcgcagtcgcccacaaccgcccacagccccgcgtcggtaDrdIFspIXbaIFspAIpQE-80L-Kan20406080100120140160
    gacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgctgggtcagtgcatcgctatcgcctcacatatgaccgaattgatacgccgtagtctcgtctaacatgactctcacgtggtatacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcAccINdeISapIBsaAIBspQIPflFIBstAPIBstZ17ITth111IpQE-80L-Kan180200220240260280300320340
    ttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcaaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtPciIColE1 oripQE-80L-Kan360380400420440460480500520
    caaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacaDrdIBciVIColE1 oripQE-80L-Kan540560580600620640660680700
    aggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggttccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccaAlwNIColE1 oripQE-80L-Kan720740760780800820840860
    atgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtColE1 oripQE-80L-Kan8809009209409609801,0001,0201,040
    gcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatggcgttaagggattttggtcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaattcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtaccgcaattccctaaaaccagtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaXmnIBspHI*272FF270ED268LM266LH264FQ262LK2601188-2003KanRColE1 oripQE-80L-Kan1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagNM258DP256ND254IG252YK250QF248LR246KQ244LS242PS240FE238GL236CN234WL232IA230LD228QY226RD224AI222GV220RG218VD216IC214GI212LK210GE208DF206IL204ND2021188-2003KanRpQE-80L-Kan1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcAjuINsiIPvuIAsiSIBpu10ILS200FD198GH196TV194VS192DP190SF188PL186LK184HM182EK180WV178QE176VP174WG172NR170ED168DF166DS164AD162VL160GN158NM156RS154QA152QA150LR148FV146RD1441188-2003KanRpQE-80L-Kan1,4201,4401,4601,4801,5001,5201,5401,560
    ctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgctgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaNsiISmaIXmaIEcoNITspMISN142FP140CN138CV136PI134SH132LR130RL128FV126AL124AD122VI120NE118GS116DP114YE112EL110VQ108FA106TK104GP102IA100TT98LL96WA94DD92PT90RI88FH86K1188-2003KanRpQE-80L-Kan1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaaClaINruIBspDII84TP82LP80MF78ET76LW74NL72RV70ME68DT66VD64NA62VS60GK58GH56KL54FL52EP50AD48PK46GY44LR42YI40TA38GS36QG34VN32DR30AW28K1188-2003KanRpQE-80L-Kan1,7601,7801,8001,8201,8401,8601,8801,9001,920
    atacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaccatgacattaacctataaaaataggcgtatcacgaggccctttcgtatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactggtactgtaattggatatttttatccgcatagtgctccgggaaagcBspHIEcoO109IY26GY24LD22AD20MN18SN16LR14PR12SC10ST8ER6QI4HS2M1188-2003KanRpQE-80L-Kan1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tcttcacctcgagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaatttcacacagaattcattaaagaggagaaattaactatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatgagaagtggagctctttagtattttttaaataaacgaaacactcgcctattgttaatattatctaagttaacactcgcctattgttaaagtgtgtcttaagtaatttctcctctttaattgataccgaaggaggcttctgcaatagtttctcaagtacgcaaagtttcaagcatacMfeIPsiITliIXhoIEcoRIPaeR7IMA2SS4ED6VI8KE10FM12RF14KV16RM182222-2935MA2SS4ED6VI8KE10FM12RF14KV16RM182222-3676mRFP1T5 promoterlac o...tor?lac operatorpQE-80L-Kan2,1202,1402,1602,1802,2002,2202,2402,260
    gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggcttccaaggcaattgccagtgctcaagctttagcttccacttccacttccagcaggcatgcttccatgggtctggcgatttgactttcaatggtttccaccaggcgacggcaagcgaaccctgtaggacaggggcgtcaaggtcatgccaaggtttcgaatgcaatttgtgggccAhdIHpaIPsrIBsiWIBsmFIBsmFIBstBIEG20SV22NG24HE26FE28IE30GE32GE34GR36PY38EG40TQ42TA44KL46KV48TK50GG52PL54PF56AW58DI60LS62PQ64FQ66YG68SK70AY72VK74HP762222-2935EG20SV22NG24HE26FE28IE30GE32GE34GR36PY38EG40TQ42TA44KL46KV48TK50GG52PL54PF56AW58DI60LS62PQ64FQ66YG68SK70AY72VK74HP762222-3676mRFP12,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtgactgtagggcctgatggactttgacaggaagggccttccaaagtttacccttgcacaatacttgaagcttctgccaccacaacaatggcaatgggtcctgaggagggacgttctgccactcaagtagatgtttcaatttgacgcaccatggttgaagggcaggctgccaggccaBstBIRsrIIAD78IP80DY82LK84LS86FP88EG90FK92WE94RV96MN98FE100DG102GV104VT106VT108QD110SS112LQ114DG116EF118IY120KV122KL124RG126TN128FP130SD132GP134V2222-2935AD78IP80DY82LK84LS86FP88EG90FK92WE94RV96MN98FE100DG102GV104VT106VT108QD110SS112LQ114DG116EF118IY120KV122KL124RG126TN128FP130SD132GP134V2222-3676mRFP12,4602,4802,5002,5202,5402,5602,5802,6002,620
    tatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacatacgtctttttttggtacccaacccttcgaaggtggcttgcatacatgggccttctgccacgagactttccactttagttttacgcagactttgactttctgccaccagtgatgctgcgacttcaattttggtggatgtaccgattttttggccaagtcgacggcccacgaatgAgeIPshAIM136QK138KT140MG142WE144AS146TE148RM150YP152ED154GA156LK158GE160IK162MR164LK166LK168DG170GH172YD174AE176VK178TT180YM182AK184KP186VQ188LP190GA192Y2222-2935MR2LK4LK6DG8GH10YD12AE14VK16TT18YM20AK22KP24VQ26LP28GA30Y2708-3676M136QK138KT140MG142WE144AS146TE148RM150YP152ED154GA156LK158GE160IK162MR164LK166LK168DG170GH172YD174AE176VK178TT180YM182AK184KP186VQ188LP190GA192Y2222-3676mRFP12,6402,6602,6802,7002,7202,7402,7602,7802,800
    aaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctatgggatccgctggctccgctgctggttctggcgaataggcatccaagggcgaggagctctttactggcgtagtaccaattttggctgtagtttgacctgtagtggagggtgttgcttctgatgtggtagcaacttgtcatgcttgcacgacttccagcagtgaggtggccacgataccctaggcgaccgaggcgacgaccaagaccgcttatccgtaggttcccgctcctcgagaaatgaccgcatcatggttAgeIBamHIBsaXISgrAIK194TD196IK198LD200IT202SH204NE206DY208TI210VE212QY214ER216AE218GR220HS222TG224AM226GS228AG230SA232AG234SG236E*2382222-2935K32TD34IK36LD38IT40SH42NE44DY46TI48VE50QY52ER54AE56GR58HS60TG62AM64GS66AG68SA70AG72SG74E*76AS78KG80EE82LF84TG86VV88P2708-3676K194TD196IK198LD200IT202SH204NE206DY208TI210VE212QY214ER216AE218GR220HS222TG224AM226GS228AG230SA232AG234SG236E*238AS240KG242EE244LF246TG248VV250P2222-3676mRFP1sfGFPmodified Waldo et al. linker2,8202,8402,8602,8802,9002,9202,9402,960
    ttctcgtagagctcgatggcgatgtaaatggccataagttttccgtacgcggcgagggcgagggcgatgcaactaacggcaagctcactctcaagtttatttgtactactggcaagctcccagtaccatggccaactctcgtaactactctgacctatggcgtacaatgtttttcaagagcatctcgagctaccgctacatttaccggtattcaaaaggcatgcgccgctcccgctcccgctacgttgattgccgttcgagtgagagttcaaataaacatgatgaccgttcgagggtcatggtaccggttgagagcattgatgagactggataccgcatgttacaaaaagBsiWII90LV92EL94DG96DV98NG100HK102FS104VR106GE108GE110GD112AT114NG116KL118TL120KF122IC124TT126GK128LP130VP132WP134TL136VT138TL140TY142GV144QC146FS1482708-3676I252LV254EL256DG258DV260NG262HK264FS266VR268GE270GE272GD274AT276NG278KL280TL282KF284IC286TT288GK290LP292VP294WP296TL298VT300TL302TY304GV306QC308FS3102222-3676sfGFP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccgctatccagatcacatgaagcaacatgatttttttaagtccgcaatgccagagggctatgtacaagagcgcactattagctttaaggatgatggcacctataagactcgcgcagaggtaaagtttgagggcgatactctcgtaaatcgcattgagctcaagggcattgattttggcgataggtctagtgtacttcgttgtactaaaaaaattcaggcgttacggtctcccgatacatgttctcgcgtgataatcgaaattcctactaccgtggatattctgagcgcgtctccatttcaaactcccgctatgagagcatttagcgtaactcgagttcccgtaactaaaaBsrGINmeAIIIRY150PD152HM154KQ156HD158FF160KS162AM164PE166GY168VQ170ER172TI174SF176KD178DG180TY182KT184RA186EV188KF190EG192DT194LV196NR198IE200LK202GI204DF2062708-3676RY312PD314HM316KQ318HD320FF322KS324AM326PE328GY330VQ332ER334TI336SF338KD340DG342TY344KT346RA348EV350KF352EG354DT356LV358NR360IE362LK364GI366DF3682222-3676sfGFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aaggaggatggcaatattctcggccataagctggagtataatttcaattcccataatgtatatattaccgcagataagcaaaagaatggcattaaggcgaattttaagattcgccataatgtggaggatggctccgtacaactcgcagatcattatcaacaaaatactccaattgttcctcctaccgttataagagccggtattcgacctcatattaaagttaagggtattacatatataatggcgtctattcgttttcttaccgtaattccgcttaaaattctaagcggtattacacctcctaccgaggcatgttgagcgtctagtaatagttgttttatgaggttaacBplIBplIMfeIBsaBIKE208DG210NI212LG214HK216LE218YN220FN222SH224NV226YI228TA230DK232QK234NG236IK238AN240FK242IR244HN246VE248DG250SV252QL254AD256HY258QQ260NT262PI2642708-3676KE370DG372NI374LG376HK378LE380YN382FN384SH386NV388YI390TA392DK394QK396NG398IK400AN402FK404IR406HN408VE410DG412SV414QL416AD418HY420QQ422NT424PI4262222-3676sfGFP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gcgatggcccagtactcctcccagataatcattatctctccactcaatccgtgctctccaaagatccaaatgagaagcgcgatcacatggtactcctggagtttgtaactgcagcaggcattactcatggcatggatgagctctataagctcgagcaccaccaccaccaccactacgctaccgggtcatgaggagggtctattagtaatagagaggtgagttaggcacgagaggtttctaggtttactcttcgcgctagtgtaccatgaggacctcaaacattgacgtcgtccgtaatgagtaccgtacctactcgagatattcgagctcgtggtggtggtggtggtgatPstIScaITliIXhoIPspXIPaeR7IGD266GP268VL270LP272DN274HY276LS278TQ280SV282LS284KD286PN288EK290RD292HM294VL296LE298FV300TA302AG304IT306HG308MD310EL312YK314LE316HH318HH320HH322*2708-3676GD428GP430VL432LP434DN436HY438LS440TQ442SV444LS446KD448PN450EK452RD454HM456VL458LE460FV462TA464AG466IT468HG470MD472EL474YK476LE478HH480HH482HH484*2222-3676sfGFP6x His Tag3,5203,5403,5603,5803,6003,6203,6403,660
    aggatccgcatgcgagctcggtaccccgggtcgacctgcagccaagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagctagcttggcgtcctaggcgtacgctcgagccatggggcccagctggacgtcggttcgaattaatcgactcgaacctgaggacaactatctaggtcattactggagtcttgaggtagacctaaacaagtcttgcgagccaacggcggcccgcaaaaaataaccactcttaggttcgatcgaaccgcAccIBlpIBmtINheIPstISalISmaISphIXmaIBamHIBfuAIBspMITspMIterminatorpQE-80L-Kan3,6803,7003,7203,7403,7603,7803,8003,8203,840
    agattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagatctaaaagtcctcgattccttcgattttacctctttttttagtgacctatatggtggcaactatatagggttaccgtagcatttcttgtaaaactccgtaaagtcagtcaacgagttacatggatattggtctggcaagtcgacctataatgccggaaaaatttctggcatttctDraIBpu10IcatpQE-80L-Kan3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgattttattcgtgttcaaaataggccggaaataagtgtaagaacgggcggactacttacgagtaggccttaaagcataccgttactttctgccactcgaccactataccctatcacaagtgggaacaatgtggcaaaaggtactcgtttgactttgcaaaagtagcgagacctcactAclIBspEIcatpQE-80L-Kan4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttctatggtgctgctaaaggccgtcaaagatgtgtatataagcgttctacaccgcacaatgccacttttggaccggataaagggatttcccaaataactcttatacaaaaagcagagtcggttagggacccactcaaagtggtcaaaactaaatttgcaccggttatacctgttgaagDraIPasIcatpQE-80L-Kan4,2204,2404,2604,2804,3004,3204,3404,360
    ttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttttttaaggcaaagcgggggcaaaagtggtacccgtttataatatgcgttccgctgttccacgactacggcgaccgctaagtccaagtagtacggcaaacactaccgaaggtacagccgtcttacgaattacttaatgttgtcatgacgctactcaccgtcccgccccgcattaaaaaaattccgtScaIcatpQE-80L-Kan4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gttattggtgcccttaaacgcctggggtaatgactctctagcttgaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccctctagattacgtgcagtcgatgataagctgtcaataaccacgggaatttgcggaccccattactgagagatcgaactccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagaggactcatcctgtttaggcgggagatctaatgcacgtcagctactattcgacaXbaIBsaAIE. coli rrnB terminatorpQE-80L-Kan4,5604,5804,6004,6204,6404,6604,6804,7004,720
    caaacatgagaattgtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagtttgtactcttaacacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactKasINarISfoIBsaHIBsaXIPluTI*Q360GS358EL356RS354VQ352RA350LQ348ML346SD344AL342AR340PS338AT336QT334NP332AL330TT328KR326KV324LS3224781-5863lacIpQE-80L-Kan4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctataggcgtggttgcHpaIBciVIEcoRVVP320LL318QN316GK314VA312QG310QS308LQ306LL304RD302VS300TQ298GL296LR294FD292QK290IT288TL286PP284IY282CS280SD278ET276DD274YG272VV270SI268DA266GV2644781-5863lacIpQE-80L-Kan4,9204,9404,9604,9805,0005,0205,0405,060
    cgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactBssHIIRL262GS260ET258IA256RM254AG252LA250MQ248DN246AV244LM242AT240PV238IG236EN234LM232QM230TQ228QF226GS224MA222SW220DG218ER216EA214IP212QI210QN208RT206L4781-5863lacIpQE-80L-Kan5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaactataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttApaIBstEIIPspOMINmeAIIIY204KH202WG200AL198RL196RA194SV192SS190LP188GA186LL184AI182QQ180HG178LA176VL174HE172VG170LR168TG166DE164HS162FI160IS158NI156PT154QD152SV150DL148F4781-5863lacIpQE-80L-Kan5,2605,2805,3005,3205,3405,3605,3805,4005,420
    taacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgaattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactBclIMluIBsaHIL146AP144VN142TC140AA138EV136AI134AD132QD130DL128PY126NI124IL122GS120VR118QA116LL114NH112VA110AK108CA106EV104GS102RE100VM98SV96VV94SA92GL90Q4781-5863lacIpQE-80L-Kan5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaaagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttD88AR86SK84IA82AV80IQ78SP76AH74LA72LS70ST68AV66GI64LL62SQ60KG58AL56QQ54AV52RN50PI48YN46LE44AM42AA40EV38KE36RT34KA32SV304781-5863lacIpQE-80L-Kan5,6205,6405,6605,6805,7005,7205,7405,760
    cgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggtgtcggaatttcggcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgtggtaagctaccacagccttaaagcAclIBstAPIHS28AQ26NV24VR22SV20TQ18YS16VG14AY12EA10VD8YL6TV4PK2V4781-5863lacIlacI (mutant) promoterpQE-80L-Kan5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggcagcgttgggtcctccgtcgcaacccaggaPpuMIEcoO109IpQE-80L-Kan5,960
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    5966 bp425045004750500052505500575025050075010001250pQE-80L-KanKanRcatlacI4781-58631188-2003iGEM14-16 (DNA)iGEM14-39 (DNA)CWB93 (pQE80L_seqrev) (DNA)FspAI,FspI,XbaI,DrdI,PflFI,Tth111I,BsaAI,AccI,BstZ17I,NdeI,BstAPIBspQI,SapI,PciIDrdIBciVIColE1 oriAlwNIBspHIXmnINsiIScaIE. coli rrnB terminatorXbaIBsaAIKasIBsaHI,BsaXI,NarI,SfoI,PluTIHpaI,BciVIEcoRVBssHIIPspOMI,ApaINmeAIII,BstEIIBclI,MluIBsaHIlacI (mutant) promoter,BstAPI,AclI,EcoO109I,PpuMI

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    5966