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pGRN_H-pUBQ1_AlteredGrnaTargetSite:NLS-tdTomato-tUBQ1
  • Sequence Map
    Editing disabled on read-only sequence.
    ttagtgagggttaattccgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagdcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgcaatcactcccaattaaggctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttchgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgM13R20406080100120140160
    tttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggSapIBspQIPvuII180200220240260280300320340
    acagaatcaggggataacgcaggaaagaacatgaaggccttgacaggatatattggcgggtaaactaagtcgctgtatgtgtttgtttgagatctgatctcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgtgtcttagtcccctattgcgtcctttcttgtacttccggaactgtcctatataaccgcccatttgattcagcgacatacacaaacaaactctagactagagtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcBglIIRB360380400420440460480500520
    cccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgcttggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaBciVI540560580600620640660680700
    tctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgt720740760780800820840860
    ggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaagaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagccttcttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccacc8809009209409609801,0001,0201,040
    tttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaDraIBspHI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tttaaatcaatctaaagtatatatgtgtaacattggtctagtgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaaaaatttagttagatttcatatatacacattgtaaccagatcactaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttDraI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaa1,4201,4401,4601,4801,5001,5201,5401,560
    acgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataBsmISspIAsiSIBpu10Isplit KAN F1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tcttctaatacctggaatgctgttttccctgggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacaacattggcaacgctacctttgccatgtttcaagaagattatggaccttacgacaaaagggaccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgttgtaaccgttgcgatggaaacggtacaaagtBsmIPasI1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gaaacaactctggcgcatcgggcttcccatacaatcggtagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggccttgagcaagacgtttcccgttgaatatggctcataacaccccttgtattctttgttgagaccgcgtagcccgaagggtatgttagccatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggaactcgttctgcaaagggcaacttataccgagtattgtggggaacataaNruI1,9401,9601,9802,0002,0202,0402,0602,0802,100
    actgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcttcccgacaacgcagaccgttccgtggcaaagtgacaaatacattcgtctgtcaaaataacaagtactactatataaaaatagaacacgttacattgtagtctctaaaactctgtgttgcaccgaaacaacttatttagcttgaaaacgactcaacttcctagtctagtgcgtagaagggctgttgcgtctggcaaggcaccgtttcBspHI2,1202,1402,1602,1802,2002,2202,2402,260
    caaaagttcaaaatcaccaactggtccacctacaacaaagctctcatcaaccgtggctccctcactttctggctggatgatggggcgattcaggcgatccccatccaacagcccgccgtcgagcgggcttttttatccccggaagcctgtggatagagggtagttatccacgtgagttttcaagttttagtggttgaccaggtggatgttgtttcgagagtagttggcaccgagggagtgaaagaccgacctactaccccgctaagtccgctaggggtaggttgtcgggcggcagctcgcccgaaaaaataggggccttcggacacctatctcccatcaataggtgcactBsaBI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaccgctaatgccccgcaaagccttgattcacggggctttccggcccgctccaaaaactatccacgtgaaatcgctaatcagggtacgtgaaatcgctaatcggagtacgtgaaatcgctaataaggtcacgtgaaatcgctaatcaaaaaggcacgtgagaacgctaatagcccttggcgattacggggcgtttcggaactaagtgccccgaaaggccgggcgaggtttttgataggtgcactttagcgattagtcccatgcactttagcgattagcctcatgcactttagcgattattccagtgcactttagcgattagtttttccgtgcactcttgcgattatcggg2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tttcagatcaacagcttgcaaacacccctcgctccggcaagtagttacagcaagtagtatgttcaattagcttttcaattatgaatatatatatcaattattggtcgcccttggcttgtggacaatgcgctacgcgcaccggctccgcccgtggacaaccgcaagcggttgcccaaaagtctagttgtcgaacgtttgtggggagcgaggccgttcatcaatgtcgttcatcatacaagttaatcgaaaagttaatacttatatatatagttaataaccagcgggaaccgaacacctgttacgcgatgcgcgtggccgaggcgggcacctgttggcgttcgccaacgggt2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccgtcgagcgccagcgcctttgcccacaacccggcggccggccgcaacagatcgttttataaatttttttttttgaaaaagaaaaagcccgaaaggcggcaacctctcgggcttctggatttccgatccccggaattagaagatcttggcaggatatattgtggtgtaacgttatggcagctcgcggtcgcggaaacgggtgttgggccgccggccggcgttgtctagcaaaatatttaaaaaaaaaaactttttctttttcgggctttccgccgttggagagcccgaagacctaaaggctaggggccttaatcttctagaaccgtcctatataacaccacattgcaataAclIBglIFseINaeIPsiIBglIINgoMIVLB2,8202,8402,8602,8802,9002,9202,9402,960
    cgatctggattttagtactggattttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactaagggtttcttatatgctcaacacatgagcgaaaccctataagaaccctaatttcccttatcgggaaactactcacacattatttatggaggctagacctaaaatcatgacctaaaaccaaaatccttaatctttaaaataactatcttcataaaatgtttatgtttatgtatgattcccaaagaatatacgagttgtgtactcgctttgggatattcttgggattaaagggaatagccctttgatgagtgtgtaataaatacctcClaIScaIBspDI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    aaaatagagagagatagatttgtagagagagactggtgatttcagcgtaccgaattaattctcccgcgaccagccgagcgagcttagcgaactgtggacgagaactgtgccaccaagcgtaaggccgttctctcgcatttgccttgctaggctcgcgcgagttgctggctgaggcttttatctctctctatctaaacatctctctctgaccactaaagtcgcatggcttaattaagagggcgctggtcggctcgctcgaatcgcttgacacctgctcttgacacggtggttcgcattccggcaagagagcgtaaacggaacgatccgagcgcgctcaacgaccgactccgBcgIBlpIBplIBbvCIBpu10INmeAIII3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gttctcgaaatcagctcttgttcggtcggcatctactctattcctttgccctcggacgagtgctggggcgtcggtttccactatcggcgagtacttctacacagccatcggtccagacggccgcgcttctgcgggcgatttgtgtacgcccgacagtcccggctccggatcggaccaagagctttagtcgagaacaagccagccgtagatgagataaggaaacgggagcctgctcacgaccccgcagccaaaggtgatagccgctcatgaagatgtgtcggtagccaggtctgccggcgcgaagacgcccgctaaacacatgcgggctgtcagggccgaggcctagcctgBplIScaIBsaHIHgR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gattgcgtcgcatcgaccctgcgcccaagctgcatcatcgaaattgccgtcaaccaagctctgatagagttggtcaagaccaatgcggagcatatacgcccggaggcgtggcgatcctgcaagctccggatgcctccgctcgaagtagcgcgtctgctgctccatacaagccaacctaacgcagcgtagctgggacgcgggttcgacgtagtagctttaacggcagttggttcgagactatctcaaccagttctggttacgcctcgtatatgcgggcctccgcaccgctaggacgttcgaggcctacggaggcgagcttcatcgcgcagacgacgaggtatgttcggttgHgR3,5203,5403,5603,5803,6003,6203,6403,660
    cacggcctccagaagaagatgttggcgacctcgtattgggaatccccgaacatcgcctcgctccagtcaatgaccgctgttatgcggccattgtccgtcaggacattgttggagccgaaatccgcgtgcacgaggtgccggacttcggggcagtcctcggcccaaagcatcagctgtgccggaggtcttcttctacaaccgctggagcataacccttaggggcttgtagcggagcgaggtcagttactggcgacaatacgccggtaacaggcagtcctgtaacaacctcggctttaggcgcacgtgctccacggcctgaagccccgtcaggagccgggtttcgtagtcgaBtgZIBstAPINmeAIIIHgR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    catcgagagcctgcgcgacggacgcactgacggtgtcgtccatcacagtttgccagtgatacacatggggatcagcaatcgcgcatatgaaatcacgccatgtagtgtattgaccgattccttgcggtccgaatgggccgaacccgctcgtctggctaagatcggccgcagcgatgtagctctcggacgcgctgcctgcgtgactgccacagcaggtagtgtcaaacggtcactatgtgtacccctagtcgttagcgcgtatactttagtgcggtacatcacataactggctaaggaacgccaggcttacccggcttgggcgagcagaccgattctagccggcgtcgctaNdeIPflFIRsrIITth111IHgR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cgcatccatagcctccgcgaccggctgaagaacagcgggcagttcggtttcaggcaggtcttgcaacgtgacaccctgtgcacggcgggagatgcaataggtcaggctctcgctgaactccccaatgtcaagcacttccggaatcgggagcgcggccgatgcaaagtgccgataagcgtaggtatcggaggcgctggccgacttcttgtcgcccgtcaagccaaagtccgtccagaacgttgcactgtgggacacgtgccgccctctacgttatccagtccgagagcgacttgaggggttacagttcgtgaaggccttagccctcgcgccggctacgtttcacggctattAsiSIBfuAIBspMIBstAPIHgR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    acataacgatctttgtagaaaccatcggcgcagctatttacccgcaggacatatccacgccctcctacatcgaagctgaaagcacgagattcttcgccctccgagagctgcatcaggtcggagacgctgtcgaacttttcgatcagaaacttctcgacagacgtcgcggtgagtttgtattgctagaaacatctttggtagccgcgtcgataaatgggcgtcctgtataggtgcgggaggatgtagcttcgactttcgtgctctaagaagcgggaggctctcgacgtagtccagcctctgcgacagcttgaaaagctagtctttgaagagctgtctgcagcgccactcaaZraIAatIIBsaHIPflFITth111IHgR4,2204,2404,2604,2804,3004,3204,3404,360
    caggctttttcatagggatcagcttgggctgtcctctccaaatgaaatgaacttccttatatagaggaagggtcttgcgaaggatagtgggattgtgcgtcatcccttacgtcagtggagatgtcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccagtccgaaaaagtatccctagtcgaacccgacaggagaggtttactttacttgaaggaatatatctccttcccagaacgcttcctatcaccctaacacgcagtagggaatgcagtcacctctacagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtBmgBICspCI35S4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgatgctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaatgatagcctttcctttatcgcaatgatggcatttgtaggagccaccttccttttctactgtcctttcgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgagctacgaggagcacccacccccaggtagaaaccctggtgacagccgtctccgtagaacttactatcggaaaggaaatagcgttactaccgtaaacatcctcggtggaaggaaaagatgacaggaaagctacttcactgtctatcgacccgttaccttaggctcctccaaagggct4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aattaccctttgttgaaaagtctcaatagccctttggtcttctgagactgtatctttgacatttttggagtaggggtacgataacattaacgtttacaatttcgcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccattaatgggaaacaacttttcagagttatcgggaaaccagaagactctgacatagaaactgtaaaaacctcatccccatgctattgtaattgcaaatgttaaagcgcggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtAclIBglI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattgggtaGGTCTCAccctATTTTGTAATATCCCGGGATATTTCACAAATTGAACATAcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacccatCCAGAGTgggaTAAAACATTATAGGGCCCTATAAAGTGTTTAACTTGTATBsaIPsrISmaIXmaIPvuIITspMIpUBQ1 (Altered gRNA target site)j5_0363...AnnealM13FPr...r4,9204,9404,9604,9805,0005,0205,0405,060
    GACTACAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTTAGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTTTTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCCGTTTTGTCGGACCTTCTTCCCTGATGTCTTAAAATCTTTTGTTTGAAAGAGAGAGAATAGAGTGGAAATAGAAAATCTCTCTTTTTCAAGCTAAAGGCCAACTGGCCTTACATAGAAACAAAAAAAACAAAACATTGTATAAAGCAAAAGGCTAAATCTAGCCTAGAGGAAAAGGCAAAACAGCCTGGAAGAAGGBsaBIpUBQ1 (Altered gRNA target site)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    GGTTTATCCGGATCTAATAATATCCATCTTAGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCCTCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCATGTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCCAAATAGGCCTAGATTATTATAGGTAGAATCTGAATCGATTCAAACCTAGACAAAAAACCAATCGAGAACAGTTAGCGGAGTAGTAGTCGTTCTTCCACTTTAAAAACTGTTTATTTAGAATCTTAGTACATCACAGAAACCTGGAACCCTTACTATCTTTGCTAAACAATATCpUBQ1 (Altered gRNA target site)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CTACTCTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCATATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCTAAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAATGCAAATTTGGCTCAGATAGCGATGAGATACATAGTCTGGGACTGGTTCTAGGTTGTTAGAGTATCCAAAACACGTATACTTTGGAAGCTGATTGCTCTTCACCAGAAAATTACTCTCTCTATAGATTTTACAATAGAATTTTCGGGTGAGTTTAGAGTTCCGTATTCCATCTTTACGTTTAAACCGAGTCTATCGNdeIEcoRVpUBQ1 (Altered gRNA target site)Al...e5,4405,4605,4805,5005,5205,5405,5605,5805,600
    TGCAATCGCACATTAAAGGCCTGTAACCTAGCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCGCCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAAGACGAAACACAAAAGatgccaaagaagaagaggaaagtcggaagtggtagtggtagtggaaACGTTAGCGTGTAATTTCCGGACATTGGATCGGGTTATAATCGTTTTGGGATCTGCGCATGTAACTGTATATATTTGGGCGGAGGAGGAACAAATCCCAAAGATGCACTCTCTTCTGCTTTGTGTTTTCtacggtttcttcttctcctttcagccttcaccatcaccatcaccttMluISspIAflIIIpUBQ1 (Altered gRNA target site)SV40NLSGS12bAl...e5,6205,6405,6605,6805,7005,7205,7405,760
    gcggtagtggcagtATGGTGAGCAAGGGCGAGGAGGTCATCAAAGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCGGCCCcgccatcaccgtcaTACCACTCGTTCCCGCTCCTCCAGTAGTTTCTCAAGTACGCGAAGTTCCACGCGTACCTCCCGAGGTACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCGCCGGGFspAIMV2SK4GE6EV8IK10EF12MR14FK16VR18ME20GS22MN24GH26EF28EI30EG32EG34EG36RP38YE40GT42QT44AK46LK48VT50KG52GP545790-7217MV2SK4GE6EV8IK10EF12MR14FK16VR18ME20GS22MN24GH26EF28EI30EG32EG34EG36RP38YE40GT42QT44AK46LK48VT50KG52GP545790-7217tdTomatoGS12b5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCATGTACGGCTCCAAGGCGTACGTGAAGCACCCCGCCGACATCCCCGATTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGTCTGGTGACCGTGACCCAGGACTCCGGACGGGAAGCGGACCCTGTAGGACAGGGGGGTCAAGTACATGCCGAGGTTCCGCATGCACTTCGTGGGGCGGCTGTAGGGGCTAATGTTCTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCAGACCACTGGCACTGGGTCCTGAGGAhdIBsiWILP56FA58WD60IL62SP64QF66MY68GS70KA72YV74KH76PA78DI80PD82YK84KL86SF88PE90GF92KW94ER96VM98NF100ED102GG104LV106TV108TQ110DS1125790-7217LP56FA58WD60IL62SP64QF66MY68GS70KA72YV74KH76PA78DI80PD82YK84KL86SF88PE90GF92KW94ER96VM98NF100ED102GG104LV106TV108TQ110DS1125790-7217tdTomato5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TCCCTGCAGGACGGCACGCTGATCTACAAGGTGAAGATGCGCGGCACCAACTTCCCCCCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGATCCACCAGGCCCTGAAGCTGAAGGACGAGGGACGTCCTGCCGTGCGACTAGATGTTCCACTTCTACGCGCCGTGGTTGAAGGGGGGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGTGGCTCGCGGACATGGGGGCGCTGCCGCACGACTTCCCGCTCTAGGTGGTCCGGGACTTCGACTTCCTGCNcoIPstISbfIEcoO109ISL114QD116GT118LI120YK122VK124MR126GT128NF130PP132DG134PV136MQ138KK140TM142GW144EA146ST148ER150LY152PR154DG156VL158KG160EI162HQ164AL166KL168KD1705790-7217SL114QD116GT118LI120YK122VK124MR126GT128NF130PP132DG134PV136MQ138KK140TM142GW144EA146ST148ER150LY152PR154DG156VL158KG160EI162HQ164AL166KL168KD1705790-7217tdTomato6,1406,1606,1806,2006,2206,2406,2606,2806,300
    GCGGCCACTACCTGGTGGAGTTCAAGACCATCTACATGGCCAAGAAGCCCGTGCAACTGCCCGGCTACTACTACGTGGACACCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCTCCGAGGGCCGCCACCACCTGTTCCTGGGGCATGGCGCCGGTGATGGACCACCTCAAGTTCTGGTAGATGTACCGGTTCTTCGGGCACGTTGACGGGCCGATGATGATGCACCTGTGGTTCGACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTCGCGAGGCTCCCGGCGGTGGTGGACAAGGACCCCGTACCAfeIAleIMscIBstXISexAIGG172HY174LV176EF178KT180IY182MA184KK186PV188QL190PG192YY194YV196DT198KL200DI202TS204HN206ED208YT210IV212EQ214YE216RS218EG220RH222HL224FL226GH228G5790-7217GG172HY174LV176EF178KT180IY182MA184KK186PV188QL190PG192YY194YV196DT198KL200DI202TS204HN206ED208YT210IV212EQ214YE216RS218EG220RH222HL224FL226GH228G5790-7217tdTomato6,3206,3406,3606,3806,4006,4206,4406,460
    CACCGGCAGCACCGGCAGCGGCAGCTCCGGCACCGCCTCCTCCGAGGACAACAACATGGCCGTCATCAAAGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCATGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCGTGGCCGTCGTGGCCGTCGCCGTCGAGGCCGTGGCGGAGGAGGCTCCTGTTGTTGTACCGGCAGTAGTTTCTCAAGTACGCGAAGTTCCACGCGTACCTCCCGAGGTACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGFspAIT230GS232TG234SG236SS238GT240AS242SE244DN246NM248AV250IK252EF254MR256FK258VR260ME262GS264MN266GH268EF270EI272EG274EG276EG278RP280YE282GT284QT286A5790-7217T230GS232TG234SG236SS238GT240AS242SE244DN246NM248AV250IK252EF254MR256FK258VR260ME262GS264MN266GH268EF270EI272EG274EG276EG278RP280YE282GT284QT286A5790-7217tdTomato6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCATGTACGGCTCCAAGGCGTACGTGAAGCACCCCGCCGACATCCCCGATTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGTTCGACTTCCACTGGTTCCCGCCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGGGTCAAGTACATGCCGAGGTTCCGCATGCACTTCGTGGGGCGGCTGTAGGGGCTAATGTTCTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCAhdIBsiWIK288LK290VT292KG294GP296LP298FA300WD302IL304SP306QF308MY310GS312KA314YV316KH318PA320DI322PD324YK326KL328SF330PE332GF334KW336ER338VM340NF342ED344G5790-7217K288LK290VT292KG294GP296LP298FA300WD302IL304SP306QF308MY310GS312KA314YV316KH318PA320DI322PD324YK326KL328SF330PE332GF334KW336ER338VM340NF342ED344G5790-7217tdTomato6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GTCTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCACGCTGATCTACAAGGTGAAGATGCGCGGCACCAACTTCCCCCCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGATCAGACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGTGCGACTAGATGTTCCACTTCTACGCGCCGTGGTTGAAGGGGGGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGTGGCTCGCGGACATGGGGGCGCTGCCGCACGACTTCCCGCTCTANcoIPstISbfIG346LV348TV350TQ352DS354SL356QD358GT360LI362YK364VK366MR368GT370NF372PP374DG376PV378MQ380KK382TM384GW386EA388ST390ER392LY394PR396DG398VL400KG402EI4045790-7217G346LV348TV350TQ352DS354SL356QD358GT360LI362YK364VK366MR368GT370NF372PP374DG376PV378MQ380KK382TM384GW386EA388ST390ER392LY394PR396DG398VL400KG402EI4045790-7217tdTomato6,8406,8606,8806,9006,9206,9406,9606,9807,000
    CCACCAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGACCATCTACATGGCCAAGAAGCCCGTGCAACTGCCCGGCTACTACTACGTGGACACCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCTCCGAGGGCGGTGGTCCGGGACTTCGACTTCCTGCCGCCGGTGATGGACCACCTCAAGTTCTGGTAGATGTACCGGTTCTTCGGGCACGTTGACGGGCCGATGATGATGCACCTGTGGTTCGACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTCGCGAGGCTCCCGAfeIAleIMscIBstXISexAIEcoO109IHQ406AL408KL410KD412GG414HY416LV418EF420KT422IY424MA426KK428PV430QL432PG434YY436YV438DT440KL442DI444TS446HN448ED450YT452IV454EQ456YE458RS460EG4625790-7217HQ406AL408KL410KD412GG414HY416LV418EF420KT422IY424MA426KK428PV430QL432PG434YY436YV438DT440KL442DI444TS446HN448ED450YT452IV454EQ456YE458RS460EG4625790-7217tdTomato7,0207,0407,0607,0807,1007,1207,1407,160
    CGCCACCACCTGTTCCTGTACGGCATGGACGAGCTGTACAAGtaaAGAGACTCTTATCAAGAATCCCATCTCTTGCTTGCttttttttGTTGTCTTCCCTTTGATAGGGTTTGtttttCTTGTTTCAGTGACTTTCTATGTTAAAAGATAATGTCAGTAAAAGGATTTGGTTTTCGCGGTGGTGGACAAGGACATGCCGTACCTGCTCGACATGTTCattTCTCTGAGAATAGTTCTTAGGGTAGAGAACGAACGaaaaaaaaCAACAGAAGGGAAACTATCCCAAACaaaaaGAACAAAGTCACTGAAAGATACAATTTTCTATTACAGTCATTTTCCTAAACCAAAAGBsrGIRH464HL466FL468YG470MD472EL474YK4765790-7217RH464HL466FL468YG470MD472EL474YK4765790-7217tUBQ1tdTomato7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TATTATTCTGAATCGATTACGGAAGATTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTATGTGATTAGGAGACGTGTTTCATTAATAAAATATAAGATCAATACATTGTTAGTAGTGATAAACTATGTACAAATTGTATTGATTGTAAAAGAAACACAATAATAAGACTTAGCTAATGCCTTCTAAGAACGAATTAAGGTTAGATATGTTCATAGCACTTTATTACTGGCAAATACACTAATCCTCTGCACAAAGTAATTATTTTATATTCTAGTTATGTAACAATCATCACTATTTGATACATGTTTAACATAACTAACATTTTCTTTGTGTClaIBmgBIBspDIBsrGIAflIIItUBQ17,3607,3807,4007,4207,4407,4607,4807,5007,520
    ATAGGTTCCttttttCTACAATATATTGTGACAGACTCTCTGTTTTAACGAATGAATTAAATTTGTCAACGaaaaaaTTAAAGAATGATGTGATCACTTTGCACCTTGGTTCATCTTCAGAGGCTTCTGCATGTTCCATGCATTGAGGCTCTCTACGGTCACAGGTTGTGAGCCGTATCCAAGGaaaaaaGATGTTATATAACACTGTCTGAGAGACAAAATTGCTTACTTAATTTAAACAGTTGCttttttAATTTCTTACTACACTAGTGAAACGTGGAACCAAGTAGAAGTCTCCGAAGACGTACAAGGTACGTAACTCCGAGAGATGCCAGTGTCCAACACTCGGCBclINspItUBQ17,5407,5607,5807,6007,6207,6407,6607,6807,700
    TTGTTAAAGACAAACAAATGgagaTGAGACCgagctccagcttttgttccctAACAATTTCTGTTTGTTTACctctACTCTGGctcgaggtcgaaaacaagggaBsaISacIEco53kItUBQ1Po...rj5_0362...Anneal7,7207,740
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    7752 bp5500600065007000750050010001500split KAN FRB5790-72175790-7217pUBQ1_AlteredGrnaTarget-f (DNA)tUBQ1-PostInsertGGLinker-r (DNA)

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    7752