Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV_GFAP hM3D(Gq)-mCherry
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagagatctaacataggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcacacagatctctagattgtatEagIMluINotIXbaIBglIIAflIIIL-ITRGF...r20406080100120140160
    tcctggtgtggagtaggggacgctgctctgacagaggctcgggggcctgagctggctctgtgagctggggaggaggcagacagccaggccttgtctgcaagcagacctggcagcattgggctggccgccccccagggcctcctcttcatgcccagtgaatgactcaccttggcacaggaccacacctcatcccctgcgacgagactgtctccgagcccccggactcgaccgagacactcgacccctcctccgtctgtcggtccggaacagacgttcgtctggaccgtcgtaacccgaccggcggggggtcccggaggagaagtacgggtcacttactgagtggaaccgtgPasIGFAP Promoter180200220240260280300320340
    agacacaatgttcggggtgggcacagtgcctgcttcccgccgcaccccagcccccctcaaatgccttccgagaagcccattgagcagggggcttgcattgcaccccagcctgacagcctggcatcttgggataaaagcagcacagccccctaggggctgcccttgctgtgtggcgtctgtgttacaagccccacccgtgtcacggacgaagggcggcgtggggtcggggggagtttacggaaggctcttcgggtaactcgtcccccgaacgtaacgtggggtcggactgtcggaccgtagaaccctattttcgtcgtgtcgggggatccccgacgggaacgacacaccgcAvrIIGFAP Promoter360380400420440460480500520
    ccaccggcggtggagaacaaggctctattcagcctgtgcccaggaaaggggatcaggggatgcccaggcatggacagtgggtggcagggggggagaggagggctgtctgcttcccagaagtccaaggacacaaatgggtgaggggagagctctccccatagctgggctgcggcccggtggccgccacctcttgttccgagataagtcggacacgggtcctttcccctagtcccctacgggtccgtacctgtcacccaccgtccccccctctcctcccgacagacgaagggtcttcaggttcctgtgtttacccactcccctctcgagaggggtatcgacccgacgccgggSacISgrAIEco53kIGFAP Promoter540560580600620640660680700
    aaccccaccccctcaggctatgccagggggtgttgccaggggcacccgggcatcgccagtctagcccactccttcataaagccctcgcatcccaggagcgagcagagccagagcaggttggagaggagacgcatcacctccgctgctcgcggggtctagagtcgacgccaccatgttggggtgggggagtccgatacggtcccccacaacggtccccgtgggcccgtagcggtcagatcgggtgaggaagtatttcgggagcgtagggtcctcgctcgtctcggtctcgtccaacctctcctctgcgtagtggaggcgacgagcgccccagatctcagctgcggtggtacAccISalIXbaIGFAP Promoter720740760780800820840860
    accttgcacaataacagtacaacctcgcctttgtttccaaacatcagctcctcctggatacacagcccctccgatgcagggctgcccccgggaaccgtcactcatttcggcagctacaatgtttctcgagcagctggcaatttctcctctccagacggtaccaccgatgaccctctggaacgtgttattgtcatgttggagcggaaacaaaggtttgtagtcgaggaggacctatgtgtcggggaggctacgtcccgacgggggcccttggcagtgagtaaagccgtcgatgttacaaagagctcgtcgaccgttaaagaggagaggtctgccatggtggctactgggagKpnITliIXhoIAcc65IPaeR7IhM3D8809009209409609801,0001,0201,040
    tgggaggtcataccgtctggcaagtggtcttcatcgctttcttaacgggcatcctggccttggtgaccatcatcggcaacatcctggtaattgtgtcatttaaggtcaacaagcagctgaagacggtcaacaactacttcctcttaagcctggcctgtgccgatctgattatcggaccctccagtatggcagaccgttcaccagaagtagcgaaagaattgcccgtaggaccggaaccactggtagtagccgttgtaggaccattaacacagtaaattccagttgttcgtcgacttctgccagttgttgatgaaggagaattcggaccggacacggctagactaatagccAflIIEcoNIhM3D1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggtcatttcaatgaatctgtttacgacctacatcatcatgaatcgatgggccttagggaacttggcctgtgacctctggcttgccattgactgcgtagccagcaatgcctctgttatgaatcttctggtcatcagctttgacagatacttttccatcacgaggccgctcacgtacccagtaaagttacttagacaaatgctggatgtagtagtacttagctacccggaatcccttgaaccggacactggagaccgaacggtaactgacgcatcggtcgttacggagacaatacttagaagaccagtagtcgaaactgtctatgaaaaggtagtgctccggcgagtgcatghM3D1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cgagccaaacgaacaacaaagagagccggtgtgatgatcggtctggcttgggtcatctcctttgtcctttgggctcctgccatcttgttctggcaatactttgttggaaagagaactgtgcctccgggagagtgcttcattcagttcctcagtgagcccaccattacttttggcagctcggtttgcttgttgtttctctcggccacactactagccagaccgaacccagtagaggaaacaggaaacccgaggacggtagaacaagaccgttatgaaacaacctttctcttgacacggaggccctctcacgaagtaagtcaaggagtcactcgggtggtaatgaaaaccgthM3D1,4201,4401,4601,4801,5001,5201,5401,560
    cagccatcgctggtttttatatgcctgtcaccattatgactattttatactggaggatctataaggaaactgaaaagcgtaccaaagagcttgctggcctgcaagcctctgggacagaggcagagacagaaaactttgtccaccccacgggcagttctcgaagctgcagcagttagtcggtagcgaccaaaaatatacggacagtggtaatactgataaaatatgacctcctagatattcctttgacttttcgcatggtttctcgaacgaccggacgttcggagaccctgtctccgtctctgtcttttgaaacaggtggggtgcccgtcaagagcttcgacgtcgtcaathM3D1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cgaacttcaacagcaaagcatgaaacgctccaacaggaggaagtatggccgctgccacttctggttcacaaccaagagctggaaacccagctccgagcagatggaccaagaccacagcagcagtgacagttggaacaacaatgatgctgctgcctccctggagaactccgcctccgcttgaagttgtcgtttcgtactttgcgaggttgtcctccttcataccggcgacggtgaagaccaagtgttggttctcgacctttgggtcgaggctcgtctacctggttctggtgtcgtcgtcactgtcaaccttgttgttactacgacgacggagggacctcttgaggcggagghM3D1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tccgacgaggaggacattggctccgagacgagagccatctactccatcgtgctcaagcttccgggtcacagcaccatcctcaactccaccaagttaccctcatcggacaacctgcaggtgcctgaggaggagctggggatggtggacttggagaggaaagccgacaagctgcaggaggctgctcctcctgtaaccgaggctctgctctcggtagatgaggtagcacgagttcgaaggcccagtgtcgtggtaggagttgaggtggttcaatgggagtagcctgttggacgtccacggactcctcctcgacccctaccacctgaacctctcctttcggctgttcgacgtccPaqCIhM3D1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cccagaagagcgtggacgatggaggcagttttccaaaaagcttctccaagcttcccatccagctagagtcagccgtggacacagctaagacttctgacgtcaactcctcagtgggtaagagcacggccactctacctctgtccttcaaggaagccactctggccaagaggtttgcgggtcttctcgcacctgctacctccgtcaaaaggtttttcgaagaggttcgaagggtaggtcgatctcagtcggcacctgtgtcgattctgaagactgcagttgaggagtcacccattctcgtgccggtgagatggagacaggaagttccttcggtgagaccggttctccaaacgSapIBspQIhM3D2,1202,1402,1602,1802,2002,2202,2402,260
    tctgaagaccagaagtcagatcactaagcggaaaaggatgtccctggtcaaggagaagaaagcggcccagaccctcagtgcgatcttgcttgccttcatcatcacttggaccccatacaacatcatggttctggtgaacaccttttgtgacagctgcatacccaaaaccttttggagacttctggtcttcagtctagtgattcgccttttcctacagggaccagttcctcttctttcgccgggtctgggagtcacgctagaacgaacggaagtagtagtgaacctggggtatgttgtagtaccaagaccacttgtggaaaacactgtcgacgtatgggttttggaaaaccEcoNIhM3D2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aatctgggctactggctgtgctacatcaacagcaccgtgaaccccgtgtgctatgctctgtgcaacaaaacattcagaaccactttcaagatgctgctgctgtgccagtgtgacaaaaaaaagaggcgcaagcagcagtaccagcagagacagtcggtcatttttcacaagcgcgttagacccgatgaccgacacgatgtagttgtcgtggcacttggggcacacgatacgagacacgttgttttgtaagtcttggtgaaagttctacgacgacgacacggtcacactgttttttttctccgcgttcgtcgtcatggtcgtctctgtcagccagtaaaaagtgttcgcgchM3D2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cacccgagcaggccttgaaggatcccccggtcgccaccatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacgtgggctcgtccggaacttcctagggggccagcggtggtaccactcgttcccgctcctcctattgtaccggtagtagttcctcaagtacgcgaagttccacgtgtacctcccgaggcacttgccggtgctcaagctctagctcccgctcccgctcccggcggggatgctcccgtgNcoIBamHImCherryhM3D2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcggtctggcggttcgacttccactggttcccaccgggggacgggaagcggaccctgtaggacaggggagtcaagtacatgccgaggttccggatgcacttcgtggggcggctgtaggggctgatgaacttcgacaggaaggggctcccgaagttcaccctcgcgcactacttgaagmCherry2,8202,8402,8602,8802,9002,9202,9402,960
    gaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgactcctgccgccgcaccactggcactgggtcctgaggagggacgtcctgccgctcaagtagatgttccacttcgacgcgccgtggttgaaggggaggctgccggggcattacgtcttcttctggtacccgaccctccggaggaggctcgcctacatggggctcctgccgcgggactNcoImCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    agggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgtcccgctctagttcgtctccgacttcgacttcctgccgccggtgatgctgcgactccagttctggtggatgttccggttcttcgggcacgtcgacgggccgcggatgttgcagttgtagttcaacctgtagtggagggtgttgctcctgatgtggtagcaccttgtcatgcttgcBsgIBbvCImCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgccgagggccgccactccaccggcggcatggacgagctgtacaagtaagaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatgcggctcccggcggtgaggtggccgccgtacctgctcgacatgttcattcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacataXcmIBsrGIEcoRIEcoRVSgrAIWPREmCherry3,3403,3603,3803,4003,4203,4403,4603,4803,500
    catgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccggtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggcWPRE3,5203,5403,5603,5803,6003,6203,6403,660
    ggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggaccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggccaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgAfeINaeITliIXhoIBglIISacIINgoMIVPaeR7IWPRE3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaactagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttgCspCIhGH polyA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagagacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctBlpIBsgINsiISphIBbvCIhGH polyA4,2204,2404,2604,2804,3004,3204,3404,360
    cggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactaBsaIEagINotIPmlIBstXIRsrIIhGH polyAR-ITR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtgR-ITR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaagcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttNaeINgoMIVf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacaagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtDraIIIf1 Origin4,9204,9404,9604,9805,0005,0205,0405,060
    ctcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattPsiI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttatacggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatat5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataaAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    caacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaaggttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    aacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaaScaIXmnIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    atgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacacctacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggPvuIAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    acgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccacAseIFspIAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    agcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatBsaIAmpR6,3206,3406,3606,3806,4006,4206,4406,460
    tatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatcatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctaacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatpUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    catacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatagtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatpUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    cctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaapUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    ttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori7,1807,2007,2207,2407,260
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7272 bp5000550060006500700050010001500hM3D

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    7272