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PHD5 (p2301Y-tOCS-pUBQ1:NLS-Venus-LucPlus-tUBQ1-pU6:pUBQ1_gRNA_Target1-tU6-pUBQ10:dCas9-Jas9-TPLRD2-tNos)
  • Sequence Map
    Editing disabled on read-only sequence.
    ACGCGTCCTGCAGGCGCGCCgaggggatcgagcccctgctgagcctcgacatgttgtcgcaaaattcgccctggacccgcccaacgatttgtcgtcactgtcaaggtttgacctgcacttcatttggggcccacatacaccaaaaaaatgctgcataattctcggggcagcaagtTGCGCAGGACGTCCGCGCGGctcccctagctcggggacgactcggagctgtacaacagcgttttaagcgggacctgggcgggttgctaaacagcagtgacagttccaaactggacgtgaagtaaaccccgggtgtatgtggtttttttacgacgtattaagagccccgtcgttcaApaIAscIBlpISbfIPspOMItOCSRareCut...cISbfI20406080100120140160
    cggttacccggccgccgtgctggaccgggttgaatggtgcccgtaactttcggtagagcggacggccaatactcaacttcaaggaatctcacccatgcgcgccggcggggaaccggagttcccttcagtgaacgttattagttcgccgctcggtgtgtcgtagatactagcccctgccaatgggccggcggcacgacctggcccaacttaccacgggcattgaaagccatctcgcctgccggttatgagttgaagttccttagagtgggtacgcgcggccgccccttggcctcaagggaagtcacttgcaataatcaagcggcgagccacacagcatctatgatcggggaMreItOCS180200220240260280300320340
    ggggccttttgaaatttgaataagatttatgtaatcagtcttttaggtttgaccggttctgccgctttttttaaaattggatttgtaataataaaacgcaattgtttgttattgtggcgctctatcatagatgtcgctataaacctattcagcacaatatattgttttcattttaccccggaaaactttaaacttattctaaatacattagtcagaaaatccaaactggccaagacggcgaaaaaaattttaacctaaacattattattttgcgttaacaaacaataacaccgcgagatagtatctacagcgatatttggataagtcgtgttatataacaaaagtaaaattOCS360380400420440460480500520
    atattgtacatataagtagtagggtacaatcagtaaattgaacggagaatattattcataaaaatacgatagtaacgggtgatatattcattagaatgaaccgaaaccggcggtaaggatctgagctacacatgctcaggttttttacaacgtgcacaacagaattgaaagcaaatataacatgtatattcatcatcccatgttagtcatttaacttgcctcttataataagtatttttatgctatcattgcccactatataagtaatcttacttggctttggccgccattcctagactcgatgtgtacgagtccaaaaaatgttgcacgtgttgtcttaactttcgttttOCS540560580600620640660680700
    tatcatgcgatcataggcgtctcgcatatctcattaaagcagCACGTGATTTTGTAATATCCCGGGATATTTCACAAATTGAACATAGACTACAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTTAGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAatagtacgctagtatccgcagagcgtatagagtaatttcgtcGTGCACTAAAACATTATAGGGCCCTATAAAGTGTTTAACTTGTATCTGATGTCTTAAAATCTTTTGTTTGAAAGAGAGAGAATAGAGTGGAAATAGAAAATCTCTCTTTTTCAAGCTAAAGGCCAACTGGCCTSmaIXmaITspMIpUBQ1tOCS720740760780800820840860
    ATGTATCTTTGTTTTTTTTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCCGTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTTAGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCCTCATCATCTACATAGAAACAAAAAAAACAAAACATTGTATAAAGCAAAAGGCTAAATCTAGCCTAGAGGAAAAGGCAAAACAGCCTGGAAGAAGGCCAAATAGGCCTAGATTATTATAGGTAGAATCTGAATCGATTCAAACCTAGACAAAAAACCAATCGAGAACAGTTAGCGGAGTAGTAGpUBQ18809009209409609801,0001,0201,040
    AGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCATGTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACTCTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCATATGAAACCTTCGACTAACGAGAAGTGGTCTTTTCGTTCTTCCACTTTAAAAACTGTTTATTTAGAATCTTAGTACATCACAGAAACCTGGAACCCTTACTATCTTTGCTAAACAATATCGATGAGATACATAGTCTGGGACTGGTTCTAGGTTGTTAGAGTATCCAAAACACGTATACTTTGGAAGCTGATTGCTCTTCACCAGAAApUBQ11,0601,0801,1001,1201,1401,1601,1801,2001,220
    TAATGAGAGAGATATCTAAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAATGCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTAGCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCGCCTCCTCCATTACTCTCTCTATAGATTTTACAATAGAATTTTCGGGTGAGTTTAGAGTTCCGTATTCCATCTTTACGTTTAAACCTTTCACCCGACCCGGAAAACACCATTTCCGGACATTGGATCGGGTTATAATCGTTTTGGGATCTGCGCATGTAACTGTATATATTTGGGCGGAGGAGGStuIpUBQ1pUBQ1_g...arget11,2401,2601,2801,3001,3201,3401,3601,3801,400
    TTGTTTAGGGTTTCTACGTGAGAGAAGACGAAACACAAAAGatgccaaagaagaagaggaaagtcggaagtggtagtggtagtggaagcggtagtggcagtgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaAACAAATCCCAAAGATGCACTCTCTTCTGCTTTGTGTTTTCtacggtttcttcttctcctttcagccttcaccatcaccatcaccttcgccatcaccgtcacactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtVENUS-N7pUBQ1GS12bSV40NLS1,4201,4401,4601,4801,5001,5201,5401,560
    caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcgactagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggacccgatgccggacgtcacgaagcgggcgatggggctggtgtacttcgtcgtgctgaagVENUS-N71,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgaccVENUS-N71,7601,7801,8001,8201,8401,8601,8801,9001,920
    agtacaactacaacagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactatcatgttgatgttgtcggtgttgcagatatagtggcggctgttcgtcttcttgccgtagttccggttgaagttctaggcggtgttgtagctcctgccgccgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatVENUS-N71,9401,9601,9802,0002,0202,0402,0602,0802,100
    cctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacggcagtggctcaggatcaggatctggatctggctctatggtcaccgacgccaaaaacataaagggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgccgtcaccgagtcctagtcctagacctagaccgagataccagtggctgcggtttttgtatttcY1VENUS-N7GS12cluc+ genepr2682,1202,1402,1602,1802,2002,2202,2402,260
    aaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatttccgggccgcggtaagataggcgaccttctaccttggcgacctctcgttgacgtattccgatacttctctatgcgggaccaaggaccttgttaacgaaaatgtctacgtgtatagctccacctgtagtgaatgcgactcatgaagctttacaggcaagccaaccgtcttcgatluc+ gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtactttgctatacccgacttatgtttagtgtcttagcagcatacgtcacttttgagagaagttaagaaatacggccacaacccgcgcaataaatagcctcaacgtcaacgcgggcgcttgctgtaaatattacttgcacttaacgagttgtcatacccgtaaagcgtcggatggcaluc+ gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccaccacaagcaaaggtttttccccaacgttttttaaaacttgcacgtttttttcgagggttagtaggttttttaataatagtacctaagattttgcctaatggtccctaaagtcagctacatgtgcaagcagtgtagagtagatggagggccaaaattacttatgctaaaacacggtluc+ gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttcctcaggaagctatccctgttctgttaacgtgactagtacttgaggagacctagatgaccagacggatttccacagcgagacggagtatcttgacggacgcactctaagagcgtacggtctctaggataaaaaccgttagtttagtaaggcctatgacgctaaaattcacaacaagluc+ gene2,8202,8402,8602,8802,9002,9202,9402,960
    cattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgtaaggtagtgccaaaaccttacaaatgatgtgagcctataaactatacacctaaagctcagcagaattacatatctaaacttcttctcgacaaagactcctcggaagtcctaatgttctaagtttcacgcgacgaccacggttgggataagaggaagaagcggttttcgtgagaluc+ gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaactaactgtttatgctaaatagattaaatgtgctttaacgaagaccaccgcgaggggagagattccttcagccccttcgccaacggttctccaaggtagacggtccatagtccgttcctatacccgagtgactctgatgtagtcgataagactaatgtgggctccccctactatttluc+ gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggcccgcgccagccatttcaacaaggtaaaaaacttcgcttccaacacctagacctatggcccttttgcgacccgcaattagtttctccgcttgacacacactctccaggatactaatacaggccaatacatttgttaggccttcgctggttgcggaactaactgttcctacctaluc+ gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgaccgatgtaagacctctgtatcgaatgaccctgcttctgcttgtgaagaagtagcaactggcggacttcagagactaattcatgtttccgatagtccaccgagggcgacttaaccttaggtagaacgaggttgtggggttgtagaagctgcgtccacagcgtccagaagggctgctPaqCIluc+ gene3,5203,5403,5603,5803,6003,6203,6403,660
    tgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaactgcggccacttgaagggcggcggcaacaacaaaacctcgtgcctttctgctactgcctttttctctagcacctaatgcagcggtcagttcattgttggcgctttttcaacgcgcctcctcaacacaaacacctgcttcatggctttccagaatggccttttgagctgcgttctluc+ gene3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaaAGAGACTCTTATCAAGAATCCCATCTCTTGCTTGCttttttttGTTGTCTTCCCTTTGATAGGGTTTGtttttCTTGTTTCAGTGACTTTCTATGTTAAAAGATAATGTCAGTAAAAGGATttttagtctctctaggagtatttccggttcttcccgcctttctagcggcacattTCTCTGAGAATAGTTCTTAGGGTAGAGAACGAACGaaaaaaaaCAACAGAAGGGAAACTATCCCAAACaaaaaGAACAAAGTCACTGAAAGATACAATTTTCTATTACAGTCATTTTCCTAtUBQ1luc+ gene3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TTGGTTTTCTATTATTCTGAATCGATTACGGAAGATTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTATGTGATTAGGAGACGTGTTTCATTAATAAAATATAAGATCAATACATTGTTAGTAGTGATAAACTATGTACAAATTGTATTGATTGTAAAAACCAAAAGATAATAAGACTTAGCTAATGCCTTCTAAGAACGAATTAAGGTTAGATATGTTCATAGCACTTTATTACTGGCAAATACACTAATCCTCTGCACAAAGTAATTATTTTATATTCTAGTTATGTAACAATCATCACTATTTGATACATGTTTAACATAACTAACATTTtUBQ14,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGAAACACAATAGGTTCCttttttCTACAATATATTGTGACAGACTCTCTGTTTTAACGAATGAATTAAATTTGTCAACGaaaaaaTTAAAGAATGATGTGATCACTTTGCACCTTGGTTCATCTTCAGAGGCTTCTGCATGTTCCATGCATTGAGGCTCTCTACGGTCACAGGTTCTTTGTGTTATCCAAGGaaaaaaGATGTTATATAACACTGTCTGAGAGACAAAATTGCTTACTTAATTTAAACAGTTGCttttttAATTTCTTACTACACTAGTGAAACGTGGAACCAAGTAGAAGTCTCCGAAGACGTACAAGGTACGTAACTCCGAGAGATGCCAGTGTCCAtUBQ14,2204,2404,2604,2804,3004,3204,3404,360
    TGTGAGCCGTTGTTAAAGACAAACAAATGTCGACGTAAAGCCTGTAGAAGAGGTTTCTAGCGAACGACACGAGTTTGAGCCTCATGAAGCTTCGTTGAACAACGGAAACTCGACTTGCCTTCCGCACAATACATCATTTCTTCTTAGCTTTTTTTCTTCTTCTTCGTTCATACAGACACTCGGCAACAATTTCTGTTTGTTTACAGCTGCATTTCGGACATCTTCTCCAAAGATCGCTTGCTGTGCTCAAACTCGGAGTACTTCGAAGCAACTTGTTGCCTTTGAGCTGAACGGAAGGCGTGTTATGTAGTAAAGAAGAATCGAAAAAAAGAAGAAGAAGCAAGTATGTCSalIHindIIIU6 promoter papertUBQ14,3804,4004,4204,4404,4604,4804,5004,5204,540
    TTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCATTCTTAAGATATGAAAAAAAAACAAATAGTCGAATGTAAAAGAACTTGGCATCGAAAGCAAAAGAAGAAAAATTGAAAGGTAAGCCTCAAAAACATAGAACAAAGTATCAAACAGGGTCCTAATCTTACTAATCCGTAGCTTGGAAGTTCTTAAACTAACTTATTTTGTAGAAGTAAGAATTCTATACU6 promoter paper4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAGCTAGAGTCGAATTCTATTAGAAGTTTTCCGGGGACCCTTAGACTTTCTTCTCTTCGTCCGGGTAAATATACCCTTTCTTGTTATCATAAAGAATATATCCGGGTAAATTCAACTTTTGTTAGAAGTTTTCAGGGTGTAGCGAATCTATTCTTTTGCTTCGACTCAAATATATGTCGATCTCAGCTTU6 promoter paper4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTAGTGATTAAAGTGGGCTGGGCCTTTTGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttTTTGCAAAATTTTCCAGATCGATTTCTTCTTCCTCTGTTCTTCGGCGTTCAATTTCTGGGTTTTTCCATCACTAATTTCACCCGACCCGGAAAACcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaAAACGTTTTAAAAGGTCTAGCTAAAGAAGAAGGAGACAAGAAGCCGCAAGTTAAAGACCCAAAAAGgRNA HandleU6 terminator paperpUBQ1_g...arget14,9204,9404,9604,9805,0005,0205,0405,060
    TCTTCGTTTTCTGTAACTGAAACCTAAAATTTGACCTAAAAAAAATCTCAAATAATATGATTCAGTGGTTTTGTACTTTTCAGTTAGTTGAGTTTTGCAGTTCCGATGAGATAAACCAATAACTTTGCTTAGATCTAATTCATTCCGTTACACCTCTGATGGAGATGGAAGGTTCAGAAGCAAAAGACATTGACTTTGGATTTTAAACTGGATTTTTTTTAGAGTTTATTATACTAAGTCACCAAAACATGAAAAGTCAATCAACTCAAAACGTCAAGGCTACTCTATTTGGTTATTGAAACGAATCTAGATTAAGTAAGGCAATGTGGAGACTACCTCTACCTTCCAAGU6 terminator paper5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TTAATAATGATGCCATTTTTTGGGTAATAATTTTGAATTAGAATCAAGGGTATAAGATTCATAATTAACATCACTTAAGCAAAGTTCGTAATATACGACCACAGGATATAATTTTTGGTACCTCAACAACAAACTTTCCATTCACCCATTTATGGATACACAGTCTAGCTCAACAAATTATTACTACGGTAAAAAACCCATTATTAAAACTTAATCTTAGTTCCCATATTCTAAGTATTAATTGTAGTGAATTCGTTTCAAGCATTATATGCTGGTGTCCTATATTAAAAACCATGGAGTTGTTGTTTGAAAGGTAAGTGGGTAAATACCTATGTGTCAGATCGAGTTGTKpnIAcc65IU6 terminator paperUBQ10 promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GAGCTTTTAACCCAAATTGGTACAATAGAATACAACTTTAGATCATAATTCTCAAAAGAAAGAGATTCCTTAGCTATTCTATCTGCCACTCCATTTCCTTCTCGGCTTGTATGCACAAGCATAAAATCCTCAAACTTGCTAAGTAGATACTTTATGTCTTGGATAATTGGATTGACTCGAAAATTGGGTTTAACCATGTTATCTTATGTTGAAATCTAGTATTAAGAGTTTTCTTTCTCTAAGGAATCGATAAGATAGACGGTGAGGTAAAGGAAGAGCCGAACATACGTGTTCGTATTTTAGGAGTTTGAACGATTCATCTATGAAATACAGAACCTATTAACCTAACTUBQ10 promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GACTTGACAAGCATAACTTTCATGTAACCAAAGACACAAGTTGCTGAGAATCCACCTCAAAAATGATCTTCCTATAATTGAATCGGGATAATGACAGCACAGCCCATCTAAGAGCCTCCACTTCTACTTCCAGCACGCTTCTTACTTTTACCACAGCTCTTGCACCTAACCATAACTGAACTGTTCGTATTGAAAGTACATTGGTTTCTGTGTTCAACGACTCTTAGGTGGAGTTTTTACTAGAAGGATATTAACTTAGCCCTATTACTGTCGTGTCGGGTAGATTCTCGGAGGTGAAGATGAAGGTCGTGCGAAGAATGAAAATGGTGTCGAGAACGTGGATTGGTATTUBQ10 promoter5,6205,6405,6605,6805,7005,7205,7405,760
    CACCTTCCCTGTATGATCGCGAAGCACCCACCCTAAGCCACATTTTAATCCTTCTGTTGGCCATGCCCCATCAAAGTTGCACTTAACCCAAGATTGTGGTGGAGCTTCCCATGTTTCTCGTCTGTCCCGACGGTGTTGTGGTTGGTGCTTTCCTTACATTCTGAGCCTCTTTCCTGTGGAAGGGACATACTAGCGCTTCGTGGGTGGGATTCGGTGTAAAATTAGGAAGACAACCGGTACGGGGTAGTTTCAACGTGAATTGGGTTCTAACACCACCTCGAAGGGTACAAAGAGCAGACAGGGCTGCCACAACACCAACCACGAAAGGAATGTAAGACTCGGAGAAAGGANruIUBQ10 promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TCTAATCCACTCATCTGCATCTTCTTGTGTCCTTACTAATACCTCATTGGTTCCAAATTCCCTCCCTTTAAGCACCAGCTCGTTTCTGTTCTTCCACAGCCTCCCAAGTATCCAAGGGACTAAAGCCTCCACATTCTTCAGATCAGGATATTCTTGTTTAAGATGTTGAACTCTAAGATTAGGTGAGTAGACGTAGAAGAACACAGGAATGATTATGGAGTAACCAAGGTTTAAGGGAGGGAAATTCGTGGTCGAGCAAAGACAAGAAGGTGTCGGAGGGTTCATAGGTTCCCTGATTTCGGAGGTGTAAGAAGTCTAGTCCTATAAGAACAAATTCTACAACTTGAGATUBQ10 promoterUBQ10f p...binding5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TGGAGGTTTGTATGAACTGATGATCTAGGACCGGATAAGTTCCCTTCTTCATAGCGAACTTATTCAAAGAATGTTTTGTGTATCATTCTTGTTACATTGTTATTAATGAAAAAATATTATTGGTCATTGGACTGAACACGAGTGTTAAATATGGACCAGGCCCCAAATAAGATCCACCTCCAAACATACTTGACTACTAGATCCTGGCCTATTCAAGGGAAGAAGTATCGCTTGAATAAGTTTCTTACAAAACACATAGTAAGAACAATGTAACAATAATTACTTTTTTATAATAACCAGTAACCTGACTTGTGCTCACAATTTATACCTGGTCCGGGGTTTATTCTAGGUBQ10 promoter6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ATTGATATATGAATTAAATAACAAGAATAAATCGAGTCACCAAACCACTTGCCTTTTTTAACGAGACTTGTTCACCAACTTGATACAAAAGTCATTATCCTATGCAAATCAATAATCATACAAAAATATCCAATAACACTAAAAAATTAAAAGAAATGGATAATTTCACAATATGTAACTATATACTTAATTTATTGTTCTTATTTAGCTCAGTGGTTTGGTGAACGGAAAAAATTGCTCTGAACAAGTGGTTGAACTATGTTTTCAGTAATAGGATACGTTTAGTTATTAGTATGTTTTTATAGGTTATTGTGATTTTTTAATTTTCTTTACCTATTAAAGTGTTATACUBQ10 promoter6,3206,3406,3606,3806,4006,4206,4406,460
    TTATACGATAAAGAAGTTACTTTTCCAAGAAATTCACTGATTTTATAAGCCCACTTGCATTAGATAAATGGCAAAAAAAAACAAAAAGGAAAAGAAATAAAGCACGAAGAATTCTAGAAAATACGAAATACGCTTCAATGCAGTGGGACCCACGGTTCAATTATTGCCAATTTTCAATATGCTATTTCTTCAATGAAAAGGTTCTTTAAGTGACTAAAATATTCGGGTGAACGTAATCTATTTACCGTTTTTTTTTGTTTTTCCTTTTCTTTATTTCGTGCTTCTTAAGATCTTTTATGCTTTATGCGAAGTTACGTCACCCTGGGTGCCAAGTTAATAACGGTTAAAAGKflIEcoRIUBQ10 promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGCTCCACCGTATATTTAAAAAATAAAACGATAATGCTAAAAAAATATAAATCGTAACGATCGTTAAATCTCAACGGCTGGATCTTATGACGACCGTTAGAAATTGTGGTTGTCGACGAGTCAGTAATAAACGGCGTCAAAGTGGTTGCAGCCGGCACACACGAGTCGTGTTTATTCGAGGTGGCATATAAATTTTTTATTTTGCTATTACGATTTTTTTATATTTAGCATTGCTAGCAATTTAGAGTTGCCGACCTAGAATACTGCTGGCAATCTTTAACACCAACAGCTGCTCAGTCATTATTTGCCGCAGTTTCACCAACGTCGGCCGTGTGTGCTCAGCACAAATASalIUBQ10 promoterUBQ10f ...inding6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CAACTCAAAGCACAAATACTTTTCCTCAACCTAAAAATAAGGCAATTAGCCAAAAACAACTTTGCGTGTAAACAACGCTCAATACACGTGTCATTTTATTATTAGCTATTGCTTCACCGCCTTAGCTTTCTCGTGACCTAGTCGTCCTCGTCTTTTCTTCTTCTTCTTCTATAAAGTTGAGTTTCGTGTTTATGAAAAGGAGTTGGATTTTTATTCCGTTAATCGGTTTTTGTTGAAACGCACATTTGTTGCGAGTTATGTGCACAGTAAAATAATAATCGATAACGAAGTGGCGGAATCGAAAGAGCACTGGATCAGCAGGAGCAGAAAAGAAGAAGAAGAAGATATTTUBQ10 promoter6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ACAATACCCAAAGAGCTCTTCTTCTTCACAATTCAGATTTCAATTTCTCAAAATCTTAAAAACTTTCTCTCAATTCTCTCTACCGTGATCAAGGTAAATTTCTGTGTTCCTTATTCTCTCAAAATCTTCGATTTTGTTTTCGTTCGATCCCAATTTCGTATATGTTCTTTGGTTTTGTTATGGGTTTCTCGAGAAGAAGAAGTGTTAAGTCTAAAGTTAAAGAGTTTTAGAATTTTTGAAAGAGAGTTAAGAGAGATGGCACTAGTTCCATTTAAAGACACAAGGAATAAGAGAGTTTTAGAAGCTAAAACAAAAGCAAGCTAGGGTTAAAGCATATACAAGAAACCAAAUBQ10 promoter7,0207,0407,0607,0807,1007,1207,1407,160
    AGATTCTGTTAATCTTAGATCGAAGACGATTTTCTGGGTTTGATCGTTAGATATCATCTTAATTCTCGATTAGGGTTTCATAGATATCATCCGATTTGTTCAAATAATTTGAGTTTTGTCGAATAATTACTCTTCGATTTGTGATTTCTATCTAGATCTGGTGTTAGTTTCTAGTTCTAAGACAATTAGAATCTAGCTTCTGCTAAAAGACCCAAACTAGCAATCTATAGTAGAATTAAGAGCTAATCCCAAAGTATCTATAGTAGGCTAAACAAGTTTATTAAACTCAAAACAGCTTATTAATGAGAAGCTAAACACTAAAGATAGATCTAGACCACAATCAAAGATCAUBQ10 promoter7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TTGTGCGATCGAATTTGTCGATTAATCTGAGTTTTTCTGATTAACAGggattctagaactagtggatctacaaaatgtctagagccccaaagaagaagagaaaagttagacccggggataagaaatactctattggtttggctatcggtacaaactctgttggttgggctgttatAACACGCTAGCTTAAACAGCTAATTAGACTCAAAAAGACTAATTGTCcctaagatcttgatcacctagatgttttacagatctcggggtttcttcttctcttttcaatctgggcccctattctttatgagataaccaaaccgatagccatgtttgagacaaccaacccgacaataSmaISpeIXmaITspMIA1P2K3K4K5R6K7V8R97434-7460dCas9_VP64mdCas9UBQ10 promoterPSNLS7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tactgatgaatacaaggttccatccaagaagttcaaggttttgggtaacactgatagacactccatcaaaaagaacttgattggtgccttgttgttcgattctggtgaaactgctgaagctactagattgaaaagaaccgctagaagaagatacaccagaagaaagaacagaatcatgactacttatgttccaaggtaggttcttcaagttccaaaacccattgtgactatctgtgaggtagtttttcttgaactaaccacggaacaacaagctaagaccactttgacgacttcgatgatctaacttttcttggcgatcttcttctatgtggtcttctttcttgtcttagdCas9_VP64mdCas97,5407,5607,5807,6007,6207,6407,6607,6807,700
    tgctacttgcaagaaatcttctccaacgaaatggccaaggttgatgattcattcttccacagattggaagaatccttcttggtcgaagaagataagaagcacgaaagacatccaatcttcggtaacatcgttgatgaagttgcttaccacgaaaagtacccaactatctaccattacgatgaacgttctttagaagaggttgctttaccggttccaactactaagtaagaaggtgtctaaccttcttaggaagaaccagcttcttctattcttcgtgctttctgtaggttagaagccattgtagcaactacttcaacgaatggtgcttttcatgggttgatagatggtaadCas9_VP64mdCas97,7207,7407,7607,7807,8007,8207,8407,860
    tgagaaagaagttggttgactctaccgataaggctgatttgagattgatctatttggctttggcccacatgattaagttcagaggtcatttcttgatcgaaggtgatttgaacccagataactccgatgttgataagttgttcatccaattagtccaaacctacaatcaattattactctttcttcaaccaactgagatggctattccgactaaactctaactagataaaccgaaaccgggtgtactaattcaagtctccagtaaagaactagcttccactaaacttgggtctattgaggctacaactattcaacaagtaggttaatcaggtttggatgttagttaataadCas9_VP64mdCas97,8807,9007,9207,9407,9607,9808,0008,0208,040
    cgaagaaaacccaatcaacgcctctggtgttgatgctaaagctattttgtctgccagattgtccaagtccagaagattagaaaatttgatcgcccaattaccaggtgaaaagaagaatggtttgttcggtaatttgattgccttgtctttgggtttgactccaaacttcaagtccgcttcttttgggttagttgcggagaccacaactacgatttcgataaaacagacggtctaacaggttcaggtcttctaatcttttaaactagcgggttaatggtccacttttcttcttaccaaacaagccattaaactaacggaacagaaacccaaactgaggtttgaagttcaggdCas9_VP64mdCas98,0608,0808,1008,1208,1408,1608,1808,2008,220
    aatttcgatttggctgaagatgccaagttgcaattatctaaggatacctacgatgacgatttggataacttgttggctcaaatcggtgatcaatacgctgatttgtttttggctgctaagaacttgtccgatgccattttgttgtccgatattttgagagtcaacaccgaaattattaaagctaaaccgacttctacggttcaacgttaatagattcctatggatgctactgctaaacctattgaacaaccgagtttagccactagttatgcgactaaacaaaaaccgacgattcttgaacaggctacggtaaaacaacaggctataaaactctcagttgtggctttaatdCas9_VP64mdCas98,2408,2608,2808,3008,3208,3408,3608,3808,400
    ctaaggctccattgtctgcctctatgatcaaaagatacgatgaacaccaccaagacttgactttgttgaaggctttggtcagacaacaattacctgaaaagtacaaagaaattttcttcgatcaatccaagaacggttacgccggttatattgatggtggtgcttctcaagaagagattccgaggtaacagacggagatactagttttctatgctacttgtggtggttctgaactgaaacaacttccgaaaccagtctgttgttaatggacttttcatgtttctttaaaagaagctagttaggttcttgccaatgcggccaatataactaccaccacgaagagttcttctdCas9_VP64mdCas98,4208,4408,4608,4808,5008,5208,5408,560
    attttacaagttcatcaagccaatcttggaaaagatggacggtactgaagaattattggtcaagttgaacagagaagatttgttgagaaagcaaagaaccttcgacaacggttctattccacatcaaattcacttgggtgaattgcacgcaattttgagaagacaagaagatttttaaaatgttcaagtagttcggttagaaccttttctacctgccatgacttcttaataaccagttcaacttgtctcttctaaacaactctttcgtttcttggaagctgttgccaagataaggtgtagtttaagtgaacccacttaacgtgcgttaaaactcttctgttcttctaaaadCas9_VP64mdCas98,5808,6008,6208,6408,6608,6808,7008,7208,740
    tatccattcttgaaggacaacagagaaaagatcgaaaagattctgaccttcagaatcccttactacgttggtccattggctagaggtaattcaagatttgcctggatgactagaaagtccgaagaaactattactccttggaacttcgaagaagttgtagataagggtgcttctgataggtaagaacttcctgttgtctcttttctagcttttctaagactggaagtcttagggaatgatgcaaccaggtaaccgatctccattaagttctaaacggacctactgatctttcaggcttctttgataatgaggaaccttgaagcttcttcaacatctattcccacgaagacdCas9_VP64mdCas98,7608,7808,8008,8208,8408,8608,8808,9008,920
    cccaatcctttattgaaagaatgaccaacttcgacaagaacttgccaaacgaaaaggttttgccaaagcactctttgttgtacgaatacttcaccgtctacaacgaattgactaaggttaagtacgtcaccgaaggtatgagaaaaccagcttttttatccggtgaacaaaagaagggttaggaaataactttcttactggttgaagctgttcttgaacggtttgcttttccaaaacggtttcgtgagaaacaacatgcttatgaagtggcagatgttgcttaactgattccaattcatgcagtggcttccatactcttttggtcgaaaaaataggccacttgttttcttBarIdCas9_VP64mdCas98,9408,9608,9809,0009,0209,0409,0609,0809,100
    ggctatcgtcgatttgttgttcaagaccaacagaaaggttactgtcaagcaattaaaagaagattacttcaagaaaatcgaatgcttcgactccgttgaaatttctggtgtcgaagatagattcaatgcctctttaggtacttaccatgacttgttgaaaatcatcaaggacaagccgatagcagctaaacaacaagttctggttgtctttccaatgacagttcgttaattttcttctaatgaagttcttttagcttacgaagctgaggcaactttaaagaccacagcttctatctaagttacggagaaatccatgaatggtactgaacaacttttagtagttcctgttcdCas9_VP64mdCas99,1209,1409,1609,1809,2009,2209,2409,260
    gatttcttggacaacgaagaaaacgaagatattttggaagatattgtcttgacattgaccttgtttgaagatagagaaatgattgaagaaagattgaaaacctacgcccacttgttcgatgataaggttatgaagcaattaaagagaagaagatacactggttggggtagattgtctaaagaacctgttgcttcttttgcttctataaaaccttctataacagaactgtaactggaacaaacttctatctctttactaacttctttctaacttttggatgcgggtgaacaagctactattccaatacttcgttaatttctcttcttctatgtgaccaaccccatctaacadCas9_VP64mdCas99,2809,3009,3209,3409,3609,3809,4009,4209,440
    ccagaaaattgattaacggtatcagagacaagcaatccggtaagaccattttggactttttgaagtctgatggtttcgctaacagaaacttcatgcaattaatccacgacgattccttgactttcaaagaagatatacaaaaggcccaagtctctggtcaaggtgattctttacaggtcttttaactaattgccatagtctctgttcgttaggccattctggtaaaacctgaaaaacttcagactaccaaagcgattgtctttgaagtacgttaattaggtgctgctaaggaactgaaagtttcttctatatgttttccgggttcagagaccagttccactaagaaatgtdCas9_VP64mdCas99,4609,4809,5009,5209,5409,5609,5809,6009,620
    tgaacatatcgctaacttggctggttctccagctattaagaagggtattttacaaaccgttaaggtcgttgacgaattggtcaaagttatgggtagacataagccagaaaacatcgttatcgaaatggctagagaaaatcaaaccacccaaaagggtcaaaagaactccagagaaacttgtatagcgattgaaccgaccaagaggtcgataattcttcccataaaatgtttggcaattccagcaactgcttaaccagtttcaatacccatctgtattcggtcttttgtagcaatagctttaccgatctcttttagtttggtgggttttcccagttttcttgaggtctcttdCas9_VP64mdCas99,6409,6609,6809,7009,7209,7409,7609,7809,800
    agaatgaagagaatcgaagaaggtatcaaagaattgggttcccaaattttgaaagaacacccagttgaaaacacccaattacaaaacgaaaagttgtacttgtactacttgcaaaacggtagagatatgtacgttgaccaagaattggacatcaacagattgtctgattacgatgtcttacttctcttagcttcttccatagtttcttaacccaagggtttaaaactttcttgtgggtcaacttttgtgggttaatgttttgcttttcaacatgaacatgatgaacgttttgccatctctatacatgcaactggttcttaacctgtagttgtctaacagactaatgctacdCas9_VP64mdCas99,8209,8409,8609,8809,9009,9209,9409,960
    ttgacgctatcgttccacaatcttttttgaaggatgactccattgacaacaaggtcttgactagatccgataagaatagaggtaagtccgataacgttccatctgaagaagtcgttaagaaaatgaagaactattggagacaattattgaacgccaagttgatcacccaaagaaaaactgcgatagcaaggtgttagaaaaaacttcctactgaggtaactgttgttccagaactgatctaggctattcttatctccattcaggctattgcaaggtagacttcttcagcaattcttttacttcttgataacctctgttaataacttgcggttcaactagtgggtttctttdCas9_VP64mdCas99,98010,00010,02010,04010,06010,08010,10010,12010,140
    gtttgacaatttgaccaaggctgaaagaggtggtttgtctgaattggataaggcaggtttcatcaaaagacaattagtagaaaccagacaaatcaccaagcacgttgctcaaattttggatagtagaatgaacactaagtacgacgaaaacgacaaattgatcagagaagttaagcaaactgttaaactggttccgactttctccaccaaacagacttaacctattccgtccaaagtagttttctgttaatcatctttggtctgtttagtggttcgtgcaacgagtttaaaacctatcatcttacttgtgattcatgctgcttttgctgtttaactagtctcttcaattcdCas9_VP64mdCas910,16010,18010,20010,22010,24010,26010,28010,30010,320
    gtcattaccttgaagtccaagttggtttccgatttcagaaaggacttccaattctacaaggtcagagaaatcaacaactaccatcatgcacatgatgcttacttgaatgctgttgttggtactgccttgattaagaagtatccaaagttggaatccgaatttgtctacggtgattcagtaatggaacttcaggttcaaccaaaggctaaagtctttcctgaaggttaagatgttccagtctctttagttgttgatggtagtacgtgtactacgaatgaacttacgacaacaaccatgacggaactaattcttcataggtttcaaccttaggcttaaacagatgccactaadCas9_VP64mdCas910,34010,36010,38010,40010,42010,44010,46010,48010,500
    acaaggtttacgacgttagaaagatgatcgccaagtccgaacaagaaattggtaaagctactgccaaatacttcttctactccaatattatgaatttctttaagaccgaaatcactttggccaacggtgaaattagaaaaagaccattgattgaaactaatggtgaaacaggtgatgttccaaatgctgcaatctttctactagcggttcaggcttgttctttaaccatttcgatgacggtttatgaagaagatgaggttataatacttaaagaaattctggctttagtgaaaccggttgccactttaatctttttctggtaactaactttgattaccactttgtccactdCas9_VP64mdCas910,52010,54010,56010,58010,60010,62010,64010,660
    aatcgtttgggataagggtagagattttgccactgttagaaaggtattgtccatgccacaagtaaacatcgtcaaaaagaccgaagttcaaactggtggtttctccaaagaatccattttgcctaagagaaactccgataagttgatcgctagaaaaaaagactgggacccaaaattagcaaaccctattcccatctctaaaacggtgacaatctttccataacaggtacggtgttcatttgtagcagtttttctggcttcaagtttgaccaccaaagaggtttcttaggtaaaacggattctctttgaggctattcaactagcgatctttttttctgaccctgggttttKflIdCas9_VP64mdCas910,68010,70010,72010,74010,76010,78010,80010,82010,840
    aagtacggtggttttgattctccaactgttgcttactctgttttggttgttgctaaggtcgaaaagggtaagagtaagaagttgaagtccgtcaaagaattattaggtatcactatcatggaaagatcctcattcgaaaagaatcctatcgactttttggaagccaagggttacattcatgccaccaaaactaagaggttgacaacgaatgagacaaaaccaacaacgattccagcttttcccattctcattcttcaacttcaggcagtttcttaataatccatagtgatagtacctttctaggagtaagcttttcttaggatagctgaaaaaccttcggttcccaatgtdCas9_VP64mdCas910,86010,88010,90010,92010,94010,96010,98011,00011,020
    aagaagtcaagaaggacttgatcattaagttgccaaagtacagtttgttcgaattggaaaatggtagaaagagaatgttggcttctgccggtgaattacaaaagggtaatgaattggctttgccatccaagtacgttaatttcttatacttggcctcccactacgaaaaattgaattcttcagttcttcctgaactagtaattcaacggtttcatgtcaaacaagcttaaccttttaccatctttctcttacaaccgaagacggccacttaatgttttcccattacttaaccgaaacggtaggttcatgcaattaaagaatatgaaccggagggtgatgctttttaacttdCas9_VP64mdCas911,04011,06011,08011,10011,12011,14011,16011,18011,200
    aggttctcctgaagataacgaacaaaagcaattatttgtcgaacaacacaagcactacttggacgaaatcattgaacaaatttccgaattttccaaaagagtcattttggctgacgccaatttggacaaagttttgtcagcttacaacaagcacagagataagccaattagagaatccaagaggacttctattgcttgttttcgttaataaacagcttgttgtgttcgtgatgaacctgctttagtaacttgtttaaaggcttaaaaggttttctcagtaaaaccgactgcggttaaacctgtttcaaaacagtcgaatgttgttcgtgtctctattcggttaatctcttdCas9_VP64mdCas911,22011,24011,26011,28011,30011,32011,34011,360
    caagctgaaaacatcattcacttgttcactttgactaacttgggtgctccagctgcttttaagtatttcgataccactatcgacagaaagagatacacctctaccaaagaagttttggacgctactttgatccaccaatctattactggtttgtacgaaactagaatcgacttgtgttcgacttttgtagtaagtgaacaagtgaaactgattgaacccacgaggtcgacgaaaattcataaagctatggtgatagctgtctttctctatgtggagatggtttcttcaaaacctgcgatgaaactaggtggttagataatgaccaaacatgctttgatcttagctgaacadCas9_VP64mdCas911,38011,40011,42011,44011,46011,48011,50011,52011,540
    ctcaattaggtggtgatGGTTCTGGATCAGGTAGTGGCTCAGGATCTcaagctcggaaagcatccttggctcggttcttggagaagcgcaaagagaggcttatgagtgcaatgccatacGctgcaggaggcggtggaagcATGTCTTCTCTTAGTAGAGAGCTCGTTTTCTTGATgagttaatccaccactaCCAAGACCTAGTCCATCACCGAGTCCTAGAgttcgagcctttcgtaggaaccgagccaagaacctcttcgcgtttctctccgaatactcacgttacggtatgCgacgtcctccgccaccttcgTACAGAAGAGAATCATCTCTCGAGCAAAAGAACTAQ1A2R3K4A5S6L7A8R9F10L11E12K13R14K15E16R17L18M19S20A21M22P23Y2411598-11669Jas-domain (JAZ9)TPLRD2L1F primerL2F primerdCas9_VP64mdCas911,56011,58011,60011,62011,64011,66011,68011,70011,720
    CTTACAGTTTCTCGATGAAGAGAAGTTTAAAGAGACTGTTCACAAGCTTGAACAAGAATCTGGGTTTTTCTTCAATATGAAGTATTTTGAGGATGAGGTTCACAATGGAAATTGGGATGAGGTTGAGAAGTATCTCTCTGGTTTCACTAAAGTAGATGATAATAGATACTCTATGGAATGTCAAAGAGCTACTTCTCTTCAAATTTCTCTGACAAGTGTTCGAACTTGTTCTTAGACCCAAAAAGAAGTTATACTTCATAAAACTCCTACTCCAAGTGTTACCTTTAACCCTACTCCAACTCTTCATAGAGAGACCAAAGTGATTTCATCTACTATTATCTATGAGATACHindIIITPLRD2CTLH domain11,74011,76011,78011,80011,82011,84011,86011,88011,900
    AAGATATTCTTCGAGATAAGAAAGCAGAAGTATCTCGAGGCTTTGGATAAGCATGATCGTCCCAAGGCTGTGGATATACTAGTGAAGGATTTGAAAGTGTTTTCAACTTTTAATGAGGAGCTTTTCAAGGAAATAACACAGCTCTTGACATTGGAGAACTTCCGGGAGAATGAACTTCTATAAGAAGCTCTATTCTTTCGTCTTCATAGAGCTCCGAAACCTATTCGTACTAGCAGGGTTCCGACACCTATATGATCACTTCCTAAACTTTCACAAAAGTTGAAAATTACTCCTCGAAAAGTTCCTTTATTGTGTCGAGAACTGTAACCTCTTGAAGGCCCTCTTACTTGSpeITPLRD2CTLH domain11,92011,94011,96011,98012,00012,02012,04012,060
    AGTTATCCAAGTATGGGGACACCAAGTCTGCAAGAGCGATCATGTTGGTGGAACTCAAAAAGCTGATTGAGGCGAATCCTTTATTCCGTGATAAATTGCAGTTCCCTACTCTTAGAAATTCAAGGCTGAGGACTTTGATCAACCAGAGCTTAAATTGGCAACACCAGCTTTGTAATCAATAGGTTCATACCCCTGTGGTTCAGACGTTCTCGCTAGTACAACCACCTTGAGTTTTTCGACTAACTCCGCTTAGGAAATAAGGCACTATTTAACGTCAAGGGATGAGAATCTTTAAGTTCCGACTCCTGAAACTAGTTGGTCTCGAATTTAACCGTTGTGGTCGAAACATTBbvCITPLRD212,08012,10012,12012,14012,16012,18012,20012,22012,240
    AAATCCAAGGCCAAATCCAGATATCAAGACTCTTTTTGTGGATCACTCTTGTGGCCCTCCAAATGGCGCACGAGCTCCATCTCCTGTGAACAATCCACTGCTTGGAGGGATACCAAAGGCTGGAGGATTTCCTCCTTTAGGCGCACACGGGCCATTTCAACCAACGGCGTCACCTTTTAGGTTCCGGTTTAGGTCTATAGTTCTGAGAAAAACACCTAGTGAGAACACCGGGAGGTTTACCGCGTGCTCGAGGTAGAGGACACTTGTTAGGTGACGAACCTCCCTATGGTTTCCGACCTCCTAAAGGAGGAAATCCGCGTGTGCCCGGTAAAGTTGGTTGCCGCAGTGGATPLRD2Pro-rich12,26012,28012,30012,32012,34012,36012,38012,40012,420
    GTTCCAACACCTCTTGCTGGTTGGATGTCTAGTCCATCCTCTGTCCCACATCCAGCTGTCTCAGCAGGAGCCATTGCACTTGGTGGTCCATCAATCCCAGCGGCGTTGAAGCACCCGAGAACTCCTCCAACTAATGCTTCTTTAGACTACCCGTCAGCAGATTCTtagCACGTGcCAAGGTTGTGGAGAACGACCAACCTACAGATCAGGTAGGAGACAGGGTGTAGGTCGACAGAGTCGTCCTCGGTAACGTGAACCACCAGGTAGTTAGGGTCGCCGCAACTTCGTGGGCTCTTGAGGAGGTTGATTACGAAGAAATCTGATGGGCAGTCGTCTAAGAatcGTGCACgDraIIITPLRD2Pro-rich12,44012,46012,48012,50012,52012,54012,56012,58012,600
    gttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacaagtttgtaaaccgttatttcaaagaattctaacttaggacaacggccagaacgctactaatagtatattaaagacaacttaatgcaattcgtacattattaattgtacattacgtactgcaataaatactctacccaaaaatactaatctcagggcgttaatatgtaaattatnos (nopaline synthase) 3'UTR (polyA signal)12,62012,64012,66012,68012,70012,72012,74012,760
    cgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggCCTGCAGGACGCGTGGCGCGCCagatccAATTAAACTATCAGTGTTTGACAGGATATATTGGcgggtaaacctaagagaaaagagcgtttattagaatgcgctatcttttgttttatatcgcgcgtttgatcctatttaatagcgcgcgccacagtagatacaatgatctagcccGGACGTCCTGCGCACCGCGCGGtctaggTTAATTTGATAGTCACAAACTGTCCTATATAACCgcccatttggattctcttttctcgcaaataatcttaAscISbfIMauBInos (nopaline synthase) 3'UTR (polyA signal)right bord...NA repeatRareCut-...scISbfI12,78012,80012,82012,84012,86012,88012,90012,92012,940
    aacggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttcccctcgggatcaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaattgcctataaattttcccgcacttttccaaataggcaagcaggtaaacatacacgtacggttggtgtcccaaggggagccctagtttcatgaaactaggttggggaggcgacgatatcacgtcagccgaagactgcaagtcacgtcggcagaagacttttgctgtacagcgtgtt12,96012,98013,00013,02013,04013,06013,08013,10013,120
    gtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggaccaggattcaatgcgctgtccgacggcgggacgggaaaaggaccgcaaaagaacagcgcacaaaatcagcgtatttcatcttatgaacgctgatcttggcctctgtaatgcggtacttgttctcgcggcggcgaccggacgacccgatacgggcgcagtcgtggctgctggtcctg13,14013,16013,18013,20013,22013,24013,26013,28013,300
    ttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgaccaactggttggttgcccggcttgacgtgcgccggccgacgtggttcgacaaaaggctcttctagtggccgtggtccgcgctggcgggcctcgaccggtcctacgaactggtggatgcgggaccgctgcaacactgtcactggtccgatctggcggaccgggcgtcgtgggcgctgg13,32013,34013,36013,38013,40013,42013,44013,460
    tactggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgcatgacctgtaacggctcgcgtaggtcctccggccgcgcccggacgcatcggaccgtctcggcacccggctgtggtggtgcggccggccggcgtaccacaactggcacaagcggccgtaacggctcaagctcgcaagggattagtagctggcgtgggcctcgcccgcgctccggcg13,48013,50013,52013,54013,56013,58013,60013,62013,640
    caaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccgttccgggctccgcacttcaaaccgggggcgggatgggagtggggccgtgtctagcgcgtgcgggcgctcgactagctggtccttccggcgtggcactttctccgccgacgtgacgaaccgcacgtagcgagctgggacatggcgcgtgaactcgcgtcgctccttcactgcggg13,66013,68013,70013,72013,74013,76013,78013,80013,820
    accgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtatggctccggtccgccgcgccacggaaggcactcctgcgtaactggctccggctgcgggaccgccggcggctcttacttgcggttctccttgttcgtactttggcgtggtcctgccggtcctgcttggcaaaaagtaatggcttctctagctccgcctctactagcgccggcccatSTA r...smid13,84013,86013,88013,90013,92013,94013,96013,98014,000
    cgtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcacaagctcggcgggcgcgtgcagagttggcacgccgacgtactttaggaccggccaaacagactacggttcgaccgccggaccggccggtcgaaccggcgacttctttggctcgcggcggcagatttttccactacacataaactcattttgtcgaacgcagtacgccagcgaSTA region from pVS1 plasmid14,02014,04014,06014,08014,10014,12014,14014,160
    gcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagcgcatatactacgctactcatttatttgtttatgcgttccccttgcgtacttccaatagcgacatgaattggtctttccgcccagtccgttctgctggtagcgttgggtagatcgggcgcgggacgttgagcggccccggctacaagacaatcagctaaggctaggggtcccgtcSTA region from pVS1 plasmid14,18014,20014,22014,24014,26014,28014,30014,32014,340
    tgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgacgggcgctaacccgccggcacgcccttctagttggcgattggcaacagccgtagctggcgggctgctaactggcgctgcacttccggtagccggccgcgctgaagcatcactagctgcctcgcggggtccgccgcctgaaccgacacaggcgctagttccgtcggctgaagcacSTA region from pVS1 plasmid14,36014,38014,40014,42014,44014,46014,48014,50014,520
    ctgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacggactaaggccacgtcggttcgggaatgctgtatacccggtggcggctggaccacctcgaccaattcgtcgcgtaactccagtgcctaccttccgatgttcgccggaaacagcacagcgcccgctagtttccgtgcgcgtagccgccactccaacggctccgcgaccggcccatgcSTA region from pVS1 plasmid14,54014,56014,58014,60014,62014,64014,66014,68014,700
    agctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgtcgacgggtaagaactcagggcatagtgcgtcgcgcactcgatgggtccgtgacggcggcggccgtgttggcaagaacttagtcttgggctcccgctgcgacgggcgctccaggtccgcgaccggcgactttaatttagttttgagtaaactcaattactccatttctcttttacSTA region from pVS1 plasmid14,72014,74014,76014,78014,80014,82014,84014,860
    agcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgtcgttttcgtgtttgtgcgattcacggccggcaggctcgcgtgcgtcgtcgttccgacgttgcaaccggtcggaccgtctgtgcggtcggtacttcgcccagttgaaagtcaacggccgcctcctagtgtggttcgacttctacatgcgccatgcggttccgttctggtaatggcSTA region from pVS1 plasmid14,88014,90014,92014,94014,96014,98015,00015,02015,040
    agctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggcttcgacgatagacttatgtagcgcgtcgatggtctcatttactcgtttacttatttactcatctacttaaaatcgccgatttcctccgccgtaccttttagttcttgttggtccgtggctgcggcaccttacggggtacacacctccttgcccgccaaccggtccgcattcgccga15,06015,08015,10015,12015,14015,16015,18015,20015,220
    gggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgacggtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgcccccaacagacggccgggacgttaccgtgaccttgggggttcgggctccttagccgcactgccagcgtttggtaggccgggccatgtttagccgcgccgcgacccactactggaccacctcttcaacttccggcgcgtccggcgggtcgccgttgcgtagctccgtcttcgtgcgg15,24015,26015,28015,30015,32015,34015,36015,38015,400
    ccggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggcggccacttagcaccgttcgccggcgactagcttaggcgtttcttagggccgttggcggccgtcggccacgcggcagctaatccttcggcgggttcccgctgctcgttggtctaaaaaagcaaggctacgagatactgcacccgtgggcgctatcagcgtcgtagtacctgcaccg15,42015,44015,46015,48015,50015,52015,54015,560
    cgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagcaaaaggcagacagcttcgcactggctgctcgaccgctccactaggcgatgctcgaaggtctgcccgtgcatctccaaaggcgtcccggccggccgtaccggtcacacaccctaatgctggaccatgactaccgccaaagggtagattggcttaggtacttggctatggcccttpVS1-REP; replication origin from pVS115,58015,60015,62015,64015,66015,68015,70015,72015,740
    gggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggcccttccctctgttcgggccggcgcacaaggcaggtgtgcaacgcctgcatgagttcaagacggccgctcggctaccgcctttcgtctttctgctggaccatctttggacgtaagccaatttgtggtgcgtgcaacggtacgtcgcatgcttcttccggttcttgccggcggaccBsiWIpVS1-REP; replication origin from pVS115,76015,78015,80015,82015,84015,86015,88015,90015,920
    tgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggactgccataggctcccacttcggaactaatcggcgatgttctagcatttctcgctttggcccgccggcctcatgtagctctagctcgatcgactaacctacatggcgctctagtgtcttccgttcttgggcctgcacgactgccaagtggggctaatgaaaaactagctagggccBmtINheIpVS1-REP; replication origin from pVS115,94015,96015,98016,00016,02016,04016,06016,08016,100
    catcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggtagccggcaaaagagatggcggaccgtgcggcgcggcgtccgttccgtcttcggtctaccaacaagttctgctagatgcttgcgtcaccgtcgcggcctctcaagttcttcaagacaaagtggcacgcgttcgactagcccagtttactggacggcctcatgctaaacttcctcFspIpVS1-REP; replication origin from pVS116,12016,14016,16016,18016,20016,22016,24016,260
    gaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattgggaacccaaagcctccgccccgtccgaccgggctaggatcagtacgcgatggcgttggactagctcccgcttcgtaggcggccaaggattacatgcctcgtctacgatcccgtttaacgggatcgtcccctttttccagcttttccagagaaaggacacctatcgtgcatgtaacccttgggtttcgBsaIpVS1-REP; replication origin from pVS116,28016,30016,32016,34016,36016,38016,40016,42016,440
    cgtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcatgtaacccttggccttgggcatgtaacccttgggtttcggcatgtaacccttggccagtgtgtacattcactgactatattttctcttttttccgctaaaaaggcggattttgagaaattttgaataattttgagaattttgggcggaccggacacgtattgacagaccggtpVS1-REP; replication origin from pVS116,46016,48016,50016,52016,54016,56016,58016,60016,620
    gcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgccgcgtgtcggcttctcgacgtttttcgcggatgggaagccagcgacgcgagggatgcggggcggcgaagcgcagccggatagcgccggcgaccggcgagtttttaccgaccggatgccggtccgttagatggtcccgcgcctgttcggcgcggcagcggtgagctggcggccgcgMreI16,64016,66016,68016,70016,72016,74016,76016,78016,800
    ccacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcaggtgtagttccgtgggacggagcgcgcaaagccactactgccacttttggagactgtgtacgtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcagtcccgcgcagtcgcccacaaccgcccacagccccgcgtcggtactgggtcagt16,82016,84016,86016,88016,90016,92016,94016,960
    cgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcatcgctatcgcctcacatatgaccgaattgatacgccgtagtctcgtctaacatgactctcacgtggtatacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgcBstZ17Ibom site from pBR32216,98017,00017,02017,04017,06017,08017,10017,12017,140
    gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctbom site from pBR32217,16017,18017,20017,22017,24017,26017,28017,30017,320
    cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtpBR322 origin of replication17,34017,36017,38017,40017,42017,44017,46017,48017,500
    gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccpBR322 origin of replication17,52017,54017,56017,58017,60017,62017,64017,660
    tgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgc17,68017,70017,72017,74017,76017,78017,80017,82017,840
    cgcagaaaaaaaggatctcaagaagaattgcgaccgtcgaggagaacttctagtatatctacatacctaatattattgccttattaaaaatggaatcccaacaattacatcaaaatccacattctcttcaaaatcaattgtcctgtacttccttgttcatgtgtgttcaaaaacggcgtctttttttcctagagttcttcttaacgctggcagctcctcttgaagatcatatagatgtatggattataataacggaataatttttaccttagggttgttaatgtagttttaggtgtaagagaagttttagttaacaggacatgaaggaacaagtacacacaagtttttgcLEU2 Promoter17,86017,88017,90017,92017,94017,96017,98018,00018,020
    ttatatttataggataattatactctatttctcaacaagtaattggttgtttggccgagcggtctaaggcgcctgattcaagaaatatcttgaccgcagttaactgtgggaatactcaggtatcgtaagatgcaagagttcgaatctcttagcaaccattatttttttcctcaacaatataaatatcctattaatatgagataaagagttgttcattaaccaacaaaccggctcgccagattccgcggactaagttctttatagaactggcgtcaattgacacccttatgagtccatagcattctacgttctcaagcttagagaatcgttggtaataaaaaaaggagttgHpaILEU2 Promoter18,04018,06018,08018,10018,12018,14018,16018,18018,200
    ataacgagaacacacaggggcgctatcgcacagaatcaaattcgatgactggaaattttttgttaatttcagaggtcgcctgacgcatatacctttttcaactgaaaaattgggagaaaaaggaaaggtgagagcgccggaaccggcttttcatatagaatagagaagcgttcattattgctcttgtgtgtccccgcgatagcgtgtcttagtttaagctactgacctttaaaaaacaattaaagtctccagcggactgcgtatatggaaaaagttgactttttaaccctctttttcctttccactctcgcggccttggccgaaaagtatatcttatctcttcgcaagtaLEU2 Promoter18,22018,24018,26018,28018,30018,32018,34018,360
    gactaaatgcttgcatcacaatacttgaagttgacaatattatttaaggacctattgttttttccaataggtggttagcaatcgtcttactttctaacttttcttaccttttacatttcagcaatatatatatatatatttcaaggatataccattctaatgtctgcccctaagactgatttacgaacgtagtgttatgaacttcaactgttataataaattcctggataacaaaaaaggttatccaccaatcgttagcagaatgaaagattgaaaagaatggaaaatgtaaagtcgttatatatatatatataaagttcctatatggtaagattacagacggggattctLEU2 PromoterLEU218,38018,40018,42018,44018,46018,48018,50018,52018,540
    agatcgtcgttttgccaggtgaccacgttggtcaagaaatcacagccgaagccattaaggttcttaaagctatttctgatgttcgttccaatgtcaagttcgatttcgaaaatcatttaattggtggtgctgctatcgatgctacaggtgttccacttccagatgaggcgctggatctagcagcaaaacggtccactggtgcaaccagttctttagtgtcggcttcggtaattccaagaatttcgataaagactacaagcaaggttacagttcaagctaaagcttttagtaaattaaccaccacgacgatagctacgatgtccacaaggtgaaggtctactccgcgacctLEU218,56018,58018,60018,62018,64018,66018,68018,70018,720
    agcctccaagaaggctgatgccgttttgttaggtgctgtgggtggtcctaaatggggaaccggtagtgttagacctgaacaaggtttactaaaaatccgtaaagaacttcaattgtacgccaacttaagaccatgtaactttgcatccgactctcttttagacttatctccaatctcggaggttcttccgactacggcaaaacaatccacgacacccaccaggatttaccccttggccatcacaatctggacttgttccaaatgatttttaggcatttcttgaagttaacatgcggttgaattctggtacattgaaacgtaggctgagagaaaatctgaatagaggttagLEU218,74018,76018,78018,80018,82018,84018,86018,88018,900
    aagccacaatttgctaaaggtactgacttcgttgttgtcagagaattagtgggaggtatttactttggtaagagaaaggaagacgatggtgatggtgtcgcttgggatagtgaacaatacaccgttccagaagtgcaaagaatcacaagaatggccgctttcatggccctacaacttcggtgttaaacgatttccatgactgaagcaacaacagtctcttaatcaccctccataaatgaaaccattctctttccttctgctaccactaccacagcgaaccctatcacttgttatgtggcaaggtcttcacgtttcttagtgttcttaccggcgaaagtaccgggatgttgLEU218,92018,94018,96018,98019,00019,02019,04019,060
    atgagccaccattgcctatttggtccttggataaagctaatgttttggcctcttcaagattatggagaaaaactgtggaggaaaccatcaagaacgaatttcctacattgaaggttcaacatcaattgattgattctgccgccatgatcctagttaagaacccaacccacctaaatactcggtggtaacggataaaccaggaacctatttcgattacaaaaccggagaagttctaatacctctttttgacacctcctttggtagttcttgcttaaaggatgtaacttccaagttgtagttaactaactaagacggcggtactaggatcaattcttgggttgggtggatttLEU219,08019,10019,12019,14019,16019,18019,20019,22019,240
    tggtattataatcaccagcaacatgtttggtgatatcatctccgatgaagcctccgttatcccaggttccttgggtttgttgccatctgcgtccttggcctctttgccagacaagaacaccgcatttggtttgtacgaaccatgccacggttctgctccagatttgccaaagaataccataatattagtggtcgttgtacaaaccactatagtagaggctacttcggaggcaatagggtccaaggaacccaaacaacggtagacgcaggaaccggagaaacggtctgttcttgtggcgtaaaccaaacatgcttggtacggtgccaagacgaggtctaaacggtttcttaLEU219,26019,28019,30019,32019,34019,36019,38019,40019,420
    aaggtcaaccctatcgccactatcttgtctgctgcaatgatgttgaaattgtcattgaacttgcctgaagaaggtaaggccattgaagatgcagttaaaaaggttttggatgcaggtatcagaactggtgatttaggtggttccaacagtaccaccgaagtcggtgatgctgtcgttccagttgggatagcggtgatagaacagacgacgttactacaactttaacagtaacttgaacggacttcttccattccggtaacttctacgtcaatttttccaaaacctacgtccatagtcttgaccactaaatccaccaaggttgtcatggtggcttcagccactacgacagcLEU219,44019,46019,48019,50019,52019,54019,56019,58019,600
    ccgaagaagttaagaaaatccttgcttaaaaagattctctttttttatgatatttgtacataaactttataaatgaaattcataatagaaacgacacgaaattacaaaatggaatatgttcatagggtagacgaaactatatacgcaatctacatacatttatcaagaaggagaaggcttcttcaattcttttaggaacgaatttttctaagagaaaaaaatactataaacatgtatttgaaatatttactttaagtattatctttgctgtgctttaatgttttaccttatacaagtatcccatctgctttgatatatgcgttagatgtatgtaaatagttcttcctcttLEU2 TerminatorLEU219,62019,64019,66019,68019,70019,72019,74019,760
    aaaggaggatgtaaaggaatacaggtaagcaaattgatactaatggctcaacgtgataaggaaaaagaattgcactttaacattaatattgacaaggaggagggcaccacacaaaaagttaggtgtaacagaaaatcatgaaactatgattcctaatttatatattggaggattttttcctcctacatttccttatgtccattcgtttaactatgattaccgagttgcactattcctttttcttaacgtgaaattgtaattataactgttcctcctcccgtggtgtgtttttcaatccacattgtcttttagtactttgatactaaggattaaatatataacctcctaaaLEU2 Terminator19,78019,80019,82019,84019,86019,88019,90019,92019,940
    tctctaaaaaaaaaaaaatacaacaaataaaaaacactcaatgacctgaccatttgatggagtttaagtcaataccttcttgaaccatttcccataatggtgaaagttccctcaagaattttactctgtcagaaacggccttaacgacgtagtcgatgacggatcgcttgcctgtagagatttttttttttttatgttgtttattttttgtgagttactggactggtaaactacctcaaattcagttatggaagaacttggtaaagggtattaccactttcaagggagttcttaaaatgagacagtctttgccggaattgctgcatcagctactgcctagcgaacggacaLEU2 TerminatorARS419,96019,98020,00020,02020,04020,06020,08020,10020,120
    aacttacacgcgcctcgtatcttttaatgatggaataatttgggaatttactctgtgtttatttatttttatgttttgtatttggattttagaaagtaaataaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgtattgaatgtgcgcggagcatagaaaattactaccttattaaacccttaaatgagacacaaataaataaaaatacaaaacataaacctaaaatctttcatttatttcttccatcttctcaatgccttacttctttttttttatttgtttccaaattttttaaagttgtttttcgcatARS420,14020,16020,18020,20020,22020,24020,26020,28020,300
    ctttacatatatatttattagacaagaaaagcagattaaatagatatacattcgattaacgataagtaaaatgtaaaatcacaggattttcgtgtgtggtcttctacacagacaagatgaaacaattcggcattaatacctgagagcaggaagagcaagataaaaggtagtatttgaaatgtatatataaataatctgttcttttcgtctaatttatctatatgtaagctaattgctattcattttacattttagtgtcctaaaagcacacaccagaagatgtgtctgttctactttgttaagccgtaattatggactctcgtccttctcgttctattttccatcataaaARS420,32020,34020,36020,38020,40020,42020,44020,460
    gttggcgatccccctagagtcttttacatcttcggaaaacaaaaactattttttctttaatttctttttttactttctatttttaatttatatatttatattaaaaaatttaaattataattatttttatagcacgtcatctgcgagctcGATCCTTTGATCTTTTCTACGGGGTcaaccgctagggggatctcagaaaatgtagaagccttttgtttttgataaaaaagaaattaaagaaaaaaatgaaagataaaaattaaatatataaatataattttttaaatttaatattaataaaaatatcgtgcagtagacgctcgagCTAGGAAACTAGAAAAGATGCCCCASwaICen6ARS420,48020,50020,52020,54020,56020,58020,60020,62020,640
    CtgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcattctaggtactaaaacaattcatccagtaaaatataatattttattttctcccaatcaggcttgatccccagtaagtcaaaaaatagctcgacatactgttcttccccgatatcctccctgatcgaGactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtacgtaagatccatgattttgttaagtaggtcattttatattataaaataaaagagggttagtccgaactaggggtcattcagttttttatcgagctgtatgacaagaaggggctataggagggactagctaminoglycoside phosphotransferase20,66020,68020,70020,72020,74020,76020,78020,80020,820
    ccggacgcagaaggcaatgtcataccacttgtccgccctgccgcttctcccaagatcaataaagccacttactttgccatctttcacaaagatgttgctgtctcccaggtcgccgtgggaaaagacaagttcctcttcgggcttttccgtctttaaaaaatcatacagctcgcgcggcctgcgtcttccgttacagtatggtgaacaggcgggacggcgaagagggttctagttatttcggtgaatgaaacggtagaaagtgtttctacaacgacagagggtccagcggcacccttttctgttcaaggagaagcccgaaaaggcagaaattttttagtatgtcgagcgcgaminoglycoside phosphotransferase20,84020,86020,88020,90020,92020,94020,96020,98021,000
    ggatctttaaatggagtgtcttcttcccagttttcgcaatccacatcggccagatcgttattcagtaagtaatccaattcggctaagcggctgtctaagctattcgtatagggacaatccgatatgtcgatggagtgaaagagcctgatgcactccgcatacagctcgataatctcctagaaatttacctcacagaagaagggtcaaaagcgttaggtgtagccggtctagcaataagtcattcattaggttaagccgattcgccgacagattcgataagcatatccctgttaggctatacagctacctcactttctcggactacgtgaggcgtatgtcgagctattagaBlpIaminoglycoside phosphotransferase21,02021,04021,06021,08021,10021,12021,14021,160
    tttcagggctttgttcatcttcatactcttccgagcaaaggacgccatcggcctcactcatgagcagattgctccagccatcatgccgttcaaagtgcaggacctttggaacaggcagctttccttccagccatagcatcatgtccttttcccgttccacatcataggtggtcccaaagtcccgaaacaagtagaagtatgagaaggctcgtttcctgcggtagccggagtgagtactcgtctaacgaggtcggtagtacggcaagtttcacgtcctggaaaccttgtccgtcgaaaggaaggtcggtatcgtagtacaggaaaagggcaaggtgtagtatccaccagggaminoglycoside phosphotransferase21,18021,20021,22021,24021,26021,28021,30021,32021,340
    tttataccggctgtccgtcatttttaaatataggttttcattttctcccaccagcttatataccttagcaggagacattccttccgtatcttttacgcagcggtatttttcgatcagttttttcaattccggtgatattctcattttagccatttattatttccttcctcttttcaaatatggccgacaggcagtaaaaatttatatccaaaagtaaaagagggtggtcgaatatatggaatcgtcctctgtaaggaaggcatagaaaatgcgtcgccataaaaagctagtcaaaaaagttaaggccactataagagtaaaatcggtaaataataaaggaaggagaaaagaminoglycoside phosphotransferase21,36021,38021,40021,42021,44021,46021,48021,50021,520
    tacagtatttaaagataccccaagaagctaattataacaagacgaactccaattcactgttccttgcattctaaaaccttaaataccagaaaacagctttttcaaagttgttttcaaagttggcgtataacatagtatcgacggagccgattttgaaaccgcggtgatcacaggcatgtcataaatttctatggggttcttcgattaatattgttctgcttgaggttaagtgacaaggaacgtaagattttggaatttatggtcttttgtcgaaaaagtttcaacaaaagtttcaaccgcatattgtatcatagctgcctcggctaaaactttggcgccactagtgtccgSacII21,54021,56021,58021,60021,62021,64021,66021,68021,700
    agcaacgctctgtcatcgttacaatcaacatgctaccctccgcgagatcatccgtgtttcaaacccggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagtctgccgccttacaacggctctcccgctgacgccgtcccggactgatgggctgcctgtattcgttgcgagacagtagcaatgttagttgtacgatgggaggcgctctagtaggcacaaagtttgggccgtcgaatcaacggcaagaaggcttatcgtagccattgtactcgtttcagacggcggaatgttgccgagagggcgactgcggcagggcctgactacccgacggacata21,72021,74021,76021,78021,80021,82021,84021,860
    cgagtggtgattttgtgccgagctgccggtcggggagctgttggctggctggtggcaggatatattgtggtgtaaacaaattgacgcttagacaacttaataacacattgcggacgtttttaatgtactgaattaacgccgaattaattcgggggatctggattttagtactggagctcaccactaaaacacggctcgacggccagcccctcgacaaccgaccgaccaccgtcctatataacaccacatttgtttaactgcgaatctgttgaattattgtgtaacgcctgcaaaaattacatgacttaattgcggcttaattaagccccctagacctaaaatcatgacctCaMV 3'UTR (polyA signal)left borde...C58 T-DNA21,88021,90021,92021,94021,96021,98022,00022,02022,040
    ttttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactaagggtttcttatatgctcaacacatgagcgaaaccctataggaaccctaattcccttatctgggaactactcacacattattatggagaaactcgagcttgtcgatcgactcaaaaccaaaatccttaatctttaaaataactatcttcataaaatgtttatgtttatgtatgattcccaaagaatatacgagttgtgtactcgctttgggatatccttgggattaagggaatagacccttgatgagtgtgtaataatacctctttgagctcgaacagctagctgagPspXICaMV 3'UTR (polyA signal)22,06022,08022,10022,12022,14022,16022,18022,20022,220
    tagctagaggatcgatccgaaccccagagtcccgctcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctatcgatctcctagctaggcttggggtctcagggcgagtcttcttgagcagttcttccgctatcttccgctacgcgacgcttagccctcgccgctatggcatttcgtgctccttcgccagtcgggtaagcggcggttcgagaagtcgttatagtgcccatcggttgcgatacagganeomycin phosphotransferase22,24022,26022,28022,30022,32022,34022,36022,38022,400
    gatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgtgtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcctatcgccaggcggtgtgggtcggccggtgtcagctacttaggtcttttcgccggtaaaaggtggtactataagccgttcgtccgtagcggtacacagtgctgctctaggagcggcagcccgtacgcgcggaactcggaccgcttgtcaagccgaccgcgctcggggactacgagRsrIIneomycin phosphotransferase22,42022,44022,46022,48022,50022,52022,54022,560
    ttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggaagcaggtctagtaggactagctgttctggccgaaggtaggctcatgcacgagcgagctacgctacaaagcgaaccaccagcttacccgtccatcggcctagttcgcatacgtcggcggcgtaacgtagtcggtactacctatgaaagagccgtcctcgttccactctactgtccneomycin phosphotransferase22,58022,60022,62022,64022,66022,68022,70022,72022,740
    agatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctggagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggctctaggacggggccgtgaagcgggttatcgtcggtcagggaagggcgaagtcactgttgcagctcgtgtcgacgcgttccttgcgggcagcaccggtcggtgctatcggcgcgacggagcaggacctcaagtaagtcccgtggcctgtccagccagaactgtttttcttggcccgFspIneomycin phosphotransferase22,76022,78022,80022,82022,84022,86022,88022,90022,920
    gcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatccccatggtcgatcgacagatctgcgaaagctcgagagagatagatttcggggacgcgactgtcggccttgtgccgccgtagtctcgtcggctaacagacaacacgggtcagtatcggcttatcggagaggtgggttcgccggcctcttggacgcacgttaggtagaacaagttaggggtaccagctagctgtctagacgctttcgagctctctctatctaaaNcoIneomycin phosphotransferaseCaM...ed22,94022,96022,98023,00023,02023,04023,06023,08023,100
    gtagagagagactggtgatttcagcgtgtcctctccaaatgaaatgaacttccttatatagaggaaggtcttgcgaaggatagtgggattgtgcgtcatcccttacgtcagtggagatatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatcatctctctctgaccactaaagtcgcacaggagaggtttactttacttgaaggaatatatctccttccagaacgcttcctatcaccctaacacgcagtagggaatgcagtcacctctatagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctaCaMV35S2; CaMV 35S promoter, duplicated23,12023,14023,16023,18023,20023,22023,24023,260
    gctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaacgatagcctttcctttatcgcaatgatggcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattcgaggagcacccacccccaggtagaaaccctggtgacagccgtctccgtagaacttgctatcggaaaggaaatagcgttactaccgtaaacatccacggtggaaggaaaagatgacaggaaaactacttcactgtctatcgacccgttaccttaggctcctccaaagggctataaPshAICaMV35S2; CaMV 35S promoter, duplicated23,28023,30023,32023,34023,36023,38023,40023,42023,440
    accctttgttgaaaagtctcaatagccctttggtcttctgagactgtatctttgatattcttggagtagacgagagtgtcgtgctccaccatgttatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgctcctcgtgggtgggggtccattgggaaacaacttttcagagttatcgggaaaccagaagactctgacatagaaactataagaacctcatctgctctcacagcacgaggtggtacaatagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctacgaggagcacccacccccaggtaCaMV35S2; CaMV 35S promoter, duplicated23,46023,48023,50023,52023,54023,56023,58023,60023,620
    ctttgggaccactgtcggcagaggcatcttgaacgatagcctttcctttatcgcaatgatggcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattaccctttgttgaaaagtctcaatgaaaccctggtgacagccgtctccgtagaacttgctatcggaaaggaaatagcgttactaccgtaaacatccacggtggaaggaaaagatgacaggaaaactacttcactgtctatcgacccgttaccttaggctcctccaaagggctataatgggaaacaacttttcagagttaPshAICaMV35S2; CaMV 35S promoter, duplicated23,64023,66023,68023,70023,72023,74023,76023,78023,800
    agccctttggtcttctgagactgtatctttgatattcttggagtagacgagagtgtcgtgctccaccatgttggcaagctgctctagccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgtcgggaaaccagaagactctgacatagaaactataagaacctcatctgctctcacagcacgaggtggtacaaccgttcgacgagatcggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcacCaMV35S2; CaMV 35S promoter, duplicated23,82023,84023,86023,88023,90023,92023,94023,960
    agcgcaacgcaattaatGTGAGTTAGCTCACTCATTAGGCAactagatcgcgttgcgttaattaCACTCAATCGAGTGAGTAATCCGTtgatct23,98024,00024,020
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    24022 bp17500200002250025005000UBQ10 promoterpr2682210-2212RareCut-MluIAscISbfI,tOCS,pUBQ1,SbfI,AscI,BlpI,PspOMI,ApaI,MreI,TspMI,XmaI,SmaIpUBQ1_gRNA_Target1,PAM,SV40NLS,GS12b,VENUS-N7,first diff from YFP,StuIGS12cluc+ genePaqCISTOPtUBQ1,SalI,HindIIIU6 promoter paperpUBQ1_gRNA_Target1,gRNA Handle,U6 terminator paperAcc65I,KpnINruIHpaILEU2 PromoterLEU2LEU2 TerminatorARS4Cen6,Cen6,SwaIaminoglycoside phosphotransferase,BlpISacIIleft border repeat from C58 T-DNA,CaMV 3'UTR (polyA signal),+3CaMV35S2; CaMV 35S promoter, duplicated,FspI,NcoI,PshAI,PshAI

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    24022