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PHD6 (p2301Y-tOCS-pUBQ1:NLS-Venus-LucPlus-tUBQ1-pU6:pUBQ1_gRNA_Target1-tU6-pUBQ10:dCas9-RGA1-TPLRD2-tNos)
  • Sequence Map
    Editing disabled on read-only sequence.
    ACGCGTCCTGCAGGCGCGCCgaggggatcgagcccctgctgagcctcgacatgttgtcgcaaaattcgccctggacccgcccaacgatttgtcgtcactgtcaaggtttgacctgcacttcatttggggcccacatacaccaaaaaaatgctgcataattctcggggcagcaagtTGCGCAGGACGTCCGCGCGGctcccctagctcggggacgactcggagctgtacaacagcgttttaagcgggacctgggcgggttgctaaacagcagtgacagttccaaactggacgtgaagtaaaccccgggtgtatgtggtttttttacgacgtattaagagccccgtcgttcaApaIAscISbfIPspOMItOCSRareCut...cISbfI20406080100120140160
    cggttacccggccgccgtgctggaccgggttgaatggtgcccgtaactttcggtagagcggacggccaatactcaacttcaaggaatctcacccatgcgcgccggcggggaaccggagttcccttcagtgaacgttattagttcgccgctcggtgtgtcgtagatactagcccctgccaatgggccggcggcacgacctggcccaacttaccacgggcattgaaagccatctcgcctgccggttatgagttgaagttccttagagtgggtacgcgcggccgccccttggcctcaagggaagtcacttgcaataatcaagcggcgagccacacagcatctatgatcggggaMreItOCS180200220240260280300320340
    ggggccttttgaaatttgaataagatttatgtaatcagtcttttaggtttgaccggttctgccgctttttttaaaattggatttgtaataataaaacgcaattgtttgttattgtggcgctctatcatagatgtcgctataaacctattcagcacaatatattgttttcattttaccccggaaaactttaaacttattctaaatacattagtcagaaaatccaaactggccaagacggcgaaaaaaattttaacctaaacattattattttgcgttaacaaacaataacaccgcgagatagtatctacagcgatatttggataagtcgtgttatataacaaaagtaaaattOCS360380400420440460480500520
    atattgtacatataagtagtagggtacaatcagtaaattgaacggagaatattattcataaaaatacgatagtaacgggtgatatattcattagaatgaaccgaaaccggcggtaaggatctgagctacacatgctcaggttttttacaacgtgcacaacagaattgaaagcaaatataacatgtatattcatcatcccatgttagtcatttaacttgcctcttataataagtatttttatgctatcattgcccactatataagtaatcttacttggctttggccgccattcctagactcgatgtgtacgagtccaaaaaatgttgcacgtgttgtcttaactttcgttttOCS540560580600620640660680700
    tatcatgcgatcataggcgtctcgcatatctcattaaagcagCACGTGATTTTGTAATATCCCGGGATATTTCACAAATTGAACATAGACTACAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTTAGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAatagtacgctagtatccgcagagcgtatagagtaatttcgtcGTGCACTAAAACATTATAGGGCCCTATAAAGTGTTTAACTTGTATCTGATGTCTTAAAATCTTTTGTTTGAAAGAGAGAGAATAGAGTGGAAATAGAAAATCTCTCTTTTTCAAGCTAAAGGCCAACTGGCCTSmaIXmaITspMIpUBQ1tOCS720740760780800820840860
    ATGTATCTTTGTTTTTTTTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCCGTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTTAGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCCTCATCATCTACATAGAAACAAAAAAAACAAAACATTGTATAAAGCAAAAGGCTAAATCTAGCCTAGAGGAAAAGGCAAAACAGCCTGGAAGAAGGCCAAATAGGCCTAGATTATTATAGGTAGAATCTGAATCGATTCAAACCTAGACAAAAAACCAATCGAGAACAGTTAGCGGAGTAGTAGpUBQ18809009209409609801,0001,0201,040
    AGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCATGTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACTCTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCATATGAAACCTTCGACTAACGAGAAGTGGTCTTTTCGTTCTTCCACTTTAAAAACTGTTTATTTAGAATCTTAGTACATCACAGAAACCTGGAACCCTTACTATCTTTGCTAAACAATATCGATGAGATACATAGTCTGGGACTGGTTCTAGGTTGTTAGAGTATCCAAAACACGTATACTTTGGAAGCTGATTGCTCTTCACCAGAAApUBQ11,0601,0801,1001,1201,1401,1601,1801,2001,220
    TAATGAGAGAGATATCTAAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAATGCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTAGCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCGCCTCCTCCATTACTCTCTCTATAGATTTTACAATAGAATTTTCGGGTGAGTTTAGAGTTCCGTATTCCATCTTTACGTTTAAACCTTTCACCCGACCCGGAAAACACCATTTCCGGACATTGGATCGGGTTATAATCGTTTTGGGATCTGCGCATGTAACTGTATATATTTGGGCGGAGGAGGStuIpUBQ1pUBQ1_g...arget11,2401,2601,2801,3001,3201,3401,3601,3801,400
    TTGTTTAGGGTTTCTACGTGAGAGAAGACGAAACACAAAAGatgccaaagaagaagaggaaagtcggaagtggtagtggtagtggaagcggtagtggcagtgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaAACAAATCCCAAAGATGCACTCTCTTCTGCTTTGTGTTTTCtacggtttcttcttctcctttcagccttcaccatcaccatcaccttcgccatcaccgtcacactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtVENUS-N7pUBQ1GS12bSV40NLS1,4201,4401,4601,4801,5001,5201,5401,560
    caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcgttcaagtcgcacaggccgctcccgctcccgctacggtggatgccgttcgactgggacttcgactagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggacccgatgccggacgtcacgaagcgggcgatggggctggtgtacttcgtcgtgctgaagVENUS-N71,5801,6001,6201,6401,6601,6801,7001,7201,740
    ttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgaagttcctcctgccgttgtaggaccccgtgttcgaccVENUS-N71,7601,7801,8001,8201,8401,8601,8801,9001,920
    agtacaactacaacagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactatcatgttgatgttgtcggtgttgcagatatagtggcggctgttcgtcttcttgccgtagttccggttgaagttctaggcggtgttgtagctcctgccgccgcacgtcgagcggctggtgatggtcgtcttgtgggggtagccgctgccggggcacgacgacgggctgttggtgatVENUS-N71,9401,9601,9802,0002,0202,0402,0602,0802,100
    cctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacggcagtggctcaggatcaggatctggatctggctctatggtcaccgacgccaaaaacataaagggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgccgtcaccgagtcctagtcctagacctagaccgagataccagtggctgcggtttttgtatttcY1VENUS-N7GS12cluc+ genepr2682,1202,1402,1602,1802,2002,2202,2402,260
    aaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatttccgggccgcggtaagataggcgaccttctaccttggcgacctctcgttgacgtattccgatacttctctatgcgggaccaaggaccttgttaacgaaaatgtctacgtgtatagctccacctgtagtgaatgcgactcatgaagctttacaggcaagccaaccgtcttcgatluc+ gene2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtactttgctatacccgacttatgtttagtgtcttagcagcatacgtcacttttgagagaagttaagaaatacggccacaacccgcgcaataaatagcctcaacgtcaacgcgggcgcttgctgtaaatattacttgcacttaacgagttgtcatacccgtaaagcgtcggatggcaluc+ gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccaccacaagcaaaggtttttccccaacgttttttaaaacttgcacgtttttttcgagggttagtaggttttttaataatagtacctaagattttgcctaatggtccctaaagtcagctacatgtgcaagcagtgtagagtagatggagggccaaaattacttatgctaaaacacggtluc+ gene2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttcctcaggaagctatccctgttctgttaacgtgactagtacttgaggagacctagatgaccagacggatttccacagcgagacggagtatcttgacggacgcactctaagagcgtacggtctctaggataaaaaccgttagtttagtaaggcctatgacgctaaaattcacaacaagluc+ gene2,8202,8402,8602,8802,9002,9202,9402,960
    cattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgtaaggtagtgccaaaaccttacaaatgatgtgagcctataaactatacacctaaagctcagcagaattacatatctaaacttcttctcgacaaagactcctcggaagtcctaatgttctaagtttcacgcgacgaccacggttgggataagaggaagaagcggttttcgtgagaluc+ gene2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaactaactgtttatgctaaatagattaaatgtgctttaacgaagaccaccgcgaggggagagattccttcagccccttcgccaacggttctccaaggtagacggtccatagtccgttcctatacccgagtgactctgatgtagtcgataagactaatgtgggctccccctactatttluc+ gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggcccgcgccagccatttcaacaaggtaaaaaacttcgcttccaacacctagacctatggcccttttgcgacccgcaattagtttctccgcttgacacacactctccaggatactaatacaggccaatacatttgttaggccttcgctggttgcggaactaactgttcctacctaluc+ gene3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgaccgatgtaagacctctgtatcgaatgaccctgcttctgcttgtgaagaagtagcaactggcggacttcagagactaattcatgtttccgatagtccaccgagggcgacttaaccttaggtagaacgaggttgtggggttgtagaagctgcgtccacagcgtccagaagggctgctPaqCIluc+ gene3,5203,5403,5603,5803,6003,6203,6403,660
    tgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaactgcggccacttgaagggcggcggcaacaacaaaacctcgtgcctttctgctactgcctttttctctagcacctaatgcagcggtcagttcattgttggcgctttttcaacgcgcctcctcaacacaaacacctgcttcatggctttccagaatggccttttgagctgcgttctluc+ gene3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaaAGAGACTCTTATCAAGAATCCCATCTCTTGCTTGCttttttttGTTGTCTTCCCTTTGATAGGGTTTGtttttCTTGTTTCAGTGACTTTCTATGTTAAAAGATAATGTCAGTAAAAGGATttttagtctctctaggagtatttccggttcttcccgcctttctagcggcacattTCTCTGAGAATAGTTCTTAGGGTAGAGAACGAACGaaaaaaaaCAACAGAAGGGAAACTATCCCAAACaaaaaGAACAAAGTCACTGAAAGATACAATTTTCTATTACAGTCATTTTCCTAtUBQ1luc+ gene3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TTGGTTTTCTATTATTCTGAATCGATTACGGAAGATTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTATGTGATTAGGAGACGTGTTTCATTAATAAAATATAAGATCAATACATTGTTAGTAGTGATAAACTATGTACAAATTGTATTGATTGTAAAAACCAAAAGATAATAAGACTTAGCTAATGCCTTCTAAGAACGAATTAAGGTTAGATATGTTCATAGCACTTTATTACTGGCAAATACACTAATCCTCTGCACAAAGTAATTATTTTATATTCTAGTTATGTAACAATCATCACTATTTGATACATGTTTAACATAACTAACATTTtUBQ14,0404,0604,0804,1004,1204,1404,1604,1804,200
    AGAAACACAATAGGTTCCttttttCTACAATATATTGTGACAGACTCTCTGTTTTAACGAATGAATTAAATTTGTCAACGaaaaaaTTAAAGAATGATGTGATCACTTTGCACCTTGGTTCATCTTCAGAGGCTTCTGCATGTTCCATGCATTGAGGCTCTCTACGGTCACAGGTTCTTTGTGTTATCCAAGGaaaaaaGATGTTATATAACACTGTCTGAGAGACAAAATTGCTTACTTAATTTAAACAGTTGCttttttAATTTCTTACTACACTAGTGAAACGTGGAACCAAGTAGAAGTCTCCGAAGACGTACAAGGTACGTAACTCCGAGAGATGCCAGTGTCCAtUBQ14,2204,2404,2604,2804,3004,3204,3404,360
    TGTGAGCCGTTGTTAAAGACAAACAAATGTCGACGTAAAGCCTGTAGAAGAGGTTTCTAGCGAACGACACGAGTTTGAGCCTCATGAAGCTTCGTTGAACAACGGAAACTCGACTTGCCTTCCGCACAATACATCATTTCTTCTTAGCTTTTTTTCTTCTTCTTCGTTCATACAGACACTCGGCAACAATTTCTGTTTGTTTACAGCTGCATTTCGGACATCTTCTCCAAAGATCGCTTGCTGTGCTCAAACTCGGAGTACTTCGAAGCAACTTGTTGCCTTTGAGCTGAACGGAAGGCGTGTTATGTAGTAAAGAAGAATCGAAAAAAAGAAGAAGAAGCAAGTATGTCSalIU6 promoter papertUBQ14,3804,4004,4204,4404,4604,4804,5004,5204,540
    TTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCATTCTTAAGATATGAAAAAAAAACAAATAGTCGAATGTAAAAGAACTTGGCATCGAAAGCAAAAGAAGAAAAATTGAAAGGTAAGCCTCAAAAACATAGAACAAAGTATCAAACAGGGTCCTAATCTTACTAATCCGTAGCTTGGAAGTTCTTAAACTAACTTATTTTGTAGAAGTAAGAATTCTATACU6 promoter paper4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAGCTAGAGTCGAATTCTATTAGAAGTTTTCCGGGGACCCTTAGACTTTCTTCTCTTCGTCCGGGTAAATATACCCTTTCTTGTTATCATAAAGAATATATCCGGGTAAATTCAACTTTTGTTAGAAGTTTTCAGGGTGTAGCGAATCTATTCTTTTGCTTCGACTCAAATATATGTCGATCTCAGCTTU6 promoter paper4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTAGTGATTAAAGTGGGCTGGGCCTTTTGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttTTTGCAAAATTTTCCAGATCGATTTCTTCTTCCTCTGTTCTTCGGCGTTCAATTTCTGGGTTTTTCCATCACTAATTTCACCCGACCCGGAAAACcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaAAACGTTTTAAAAGGTCTAGCTAAAGAAGAAGGAGACAAGAAGCCGCAAGTTAAAGACCCAAAAAGgRNA HandleU6 terminator paperpUBQ1_g...arget14,9204,9404,9604,9805,0005,0205,0405,060
    TCTTCGTTTTCTGTAACTGAAACCTAAAATTTGACCTAAAAAAAATCTCAAATAATATGATTCAGTGGTTTTGTACTTTTCAGTTAGTTGAGTTTTGCAGTTCCGATGAGATAAACCAATAACTTTGCTTAGATCTAATTCATTCCGTTACACCTCTGATGGAGATGGAAGGTTCAGAAGCAAAAGACATTGACTTTGGATTTTAAACTGGATTTTTTTTAGAGTTTATTATACTAAGTCACCAAAACATGAAAAGTCAATCAACTCAAAACGTCAAGGCTACTCTATTTGGTTATTGAAACGAATCTAGATTAAGTAAGGCAATGTGGAGACTACCTCTACCTTCCAAGU6 terminator paper5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TTAATAATGATGCCATTTTTTGGGTAATAATTTTGAATTAGAATCAAGGGTATAAGATTCATAATTAACATCACTTAAGCAAAGTTCGTAATATACGACCACAGGATATAATTTTTGGTACCTCAACAACAAACTTTCCATTCACCCATTTATGGATACACAGTCTAGCTCAACAAATTATTACTACGGTAAAAAACCCATTATTAAAACTTAATCTTAGTTCCCATATTCTAAGTATTAATTGTAGTGAATTCGTTTCAAGCATTATATGCTGGTGTCCTATATTAAAAACCATGGAGTTGTTGTTTGAAAGGTAAGTGGGTAAATACCTATGTGTCAGATCGAGTTGTKpnIAcc65IU6 terminator paperUBQ10 promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GAGCTTTTAACCCAAATTGGTACAATAGAATACAACTTTAGATCATAATTCTCAAAAGAAAGAGATTCCTTAGCTATTCTATCTGCCACTCCATTTCCTTCTCGGCTTGTATGCACAAGCATAAAATCCTCAAACTTGCTAAGTAGATACTTTATGTCTTGGATAATTGGATTGACTCGAAAATTGGGTTTAACCATGTTATCTTATGTTGAAATCTAGTATTAAGAGTTTTCTTTCTCTAAGGAATCGATAAGATAGACGGTGAGGTAAAGGAAGAGCCGAACATACGTGTTCGTATTTTAGGAGTTTGAACGATTCATCTATGAAATACAGAACCTATTAACCTAACTUBQ10 promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GACTTGACAAGCATAACTTTCATGTAACCAAAGACACAAGTTGCTGAGAATCCACCTCAAAAATGATCTTCCTATAATTGAATCGGGATAATGACAGCACAGCCCATCTAAGAGCCTCCACTTCTACTTCCAGCACGCTTCTTACTTTTACCACAGCTCTTGCACCTAACCATAACTGAACTGTTCGTATTGAAAGTACATTGGTTTCTGTGTTCAACGACTCTTAGGTGGAGTTTTTACTAGAAGGATATTAACTTAGCCCTATTACTGTCGTGTCGGGTAGATTCTCGGAGGTGAAGATGAAGGTCGTGCGAAGAATGAAAATGGTGTCGAGAACGTGGATTGGTATTUBQ10 promoter5,6205,6405,6605,6805,7005,7205,7405,760
    CACCTTCCCTGTATGATCGCGAAGCACCCACCCTAAGCCACATTTTAATCCTTCTGTTGGCCATGCCCCATCAAAGTTGCACTTAACCCAAGATTGTGGTGGAGCTTCCCATGTTTCTCGTCTGTCCCGACGGTGTTGTGGTTGGTGCTTTCCTTACATTCTGAGCCTCTTTCCTGTGGAAGGGACATACTAGCGCTTCGTGGGTGGGATTCGGTGTAAAATTAGGAAGACAACCGGTACGGGGTAGTTTCAACGTGAATTGGGTTCTAACACCACCTCGAAGGGTACAAAGAGCAGACAGGGCTGCCACAACACCAACCACGAAAGGAATGTAAGACTCGGAGAAAGGANruIUBQ10 promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TCTAATCCACTCATCTGCATCTTCTTGTGTCCTTACTAATACCTCATTGGTTCCAAATTCCCTCCCTTTAAGCACCAGCTCGTTTCTGTTCTTCCACAGCCTCCCAAGTATCCAAGGGACTAAAGCCTCCACATTCTTCAGATCAGGATATTCTTGTTTAAGATGTTGAACTCTAAGATTAGGTGAGTAGACGTAGAAGAACACAGGAATGATTATGGAGTAACCAAGGTTTAAGGGAGGGAAATTCGTGGTCGAGCAAAGACAAGAAGGTGTCGGAGGGTTCATAGGTTCCCTGATTTCGGAGGTGTAAGAAGTCTAGTCCTATAAGAACAAATTCTACAACTTGAGATUBQ10 promoterUBQ10f p...binding5,9605,9806,0006,0206,0406,0606,0806,1006,120
    TGGAGGTTTGTATGAACTGATGATCTAGGACCGGATAAGTTCCCTTCTTCATAGCGAACTTATTCAAAGAATGTTTTGTGTATCATTCTTGTTACATTGTTATTAATGAAAAAATATTATTGGTCATTGGACTGAACACGAGTGTTAAATATGGACCAGGCCCCAAATAAGATCCACCTCCAAACATACTTGACTACTAGATCCTGGCCTATTCAAGGGAAGAAGTATCGCTTGAATAAGTTTCTTACAAAACACATAGTAAGAACAATGTAACAATAATTACTTTTTTATAATAACCAGTAACCTGACTTGTGCTCACAATTTATACCTGGTCCGGGGTTTATTCTAGGUBQ10 promoter6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ATTGATATATGAATTAAATAACAAGAATAAATCGAGTCACCAAACCACTTGCCTTTTTTAACGAGACTTGTTCACCAACTTGATACAAAAGTCATTATCCTATGCAAATCAATAATCATACAAAAATATCCAATAACACTAAAAAATTAAAAGAAATGGATAATTTCACAATATGTAACTATATACTTAATTTATTGTTCTTATTTAGCTCAGTGGTTTGGTGAACGGAAAAAATTGCTCTGAACAAGTGGTTGAACTATGTTTTCAGTAATAGGATACGTTTAGTTATTAGTATGTTTTTATAGGTTATTGTGATTTTTTAATTTTCTTTACCTATTAAAGTGTTATACUBQ10 promoter6,3206,3406,3606,3806,4006,4206,4406,460
    TTATACGATAAAGAAGTTACTTTTCCAAGAAATTCACTGATTTTATAAGCCCACTTGCATTAGATAAATGGCAAAAAAAAACAAAAAGGAAAAGAAATAAAGCACGAAGAATTCTAGAAAATACGAAATACGCTTCAATGCAGTGGGACCCACGGTTCAATTATTGCCAATTTTCAATATGCTATTTCTTCAATGAAAAGGTTCTTTAAGTGACTAAAATATTCGGGTGAACGTAATCTATTTACCGTTTTTTTTTGTTTTTCCTTTTCTTTATTTCGTGCTTCTTAAGATCTTTTATGCTTTATGCGAAGTTACGTCACCCTGGGTGCCAAGTTAATAACGGTTAAAAGKflIEcoRIUBQ10 promoter6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGCTCCACCGTATATTTAAAAAATAAAACGATAATGCTAAAAAAATATAAATCGTAACGATCGTTAAATCTCAACGGCTGGATCTTATGACGACCGTTAGAAATTGTGGTTGTCGACGAGTCAGTAATAAACGGCGTCAAAGTGGTTGCAGCCGGCACACACGAGTCGTGTTTATTCGAGGTGGCATATAAATTTTTTATTTTGCTATTACGATTTTTTTATATTTAGCATTGCTAGCAATTTAGAGTTGCCGACCTAGAATACTGCTGGCAATCTTTAACACCAACAGCTGCTCAGTCATTATTTGCCGCAGTTTCACCAACGTCGGCCGTGTGTGCTCAGCACAAATASalIUBQ10 promoterUBQ10f ...inding6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CAACTCAAAGCACAAATACTTTTCCTCAACCTAAAAATAAGGCAATTAGCCAAAAACAACTTTGCGTGTAAACAACGCTCAATACACGTGTCATTTTATTATTAGCTATTGCTTCACCGCCTTAGCTTTCTCGTGACCTAGTCGTCCTCGTCTTTTCTTCTTCTTCTTCTATAAAGTTGAGTTTCGTGTTTATGAAAAGGAGTTGGATTTTTATTCCGTTAATCGGTTTTTGTTGAAACGCACATTTGTTGCGAGTTATGTGCACAGTAAAATAATAATCGATAACGAAGTGGCGGAATCGAAAGAGCACTGGATCAGCAGGAGCAGAAAAGAAGAAGAAGAAGATATTTUBQ10 promoter6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ACAATACCCAAAGAGCTCTTCTTCTTCACAATTCAGATTTCAATTTCTCAAAATCTTAAAAACTTTCTCTCAATTCTCTCTACCGTGATCAAGGTAAATTTCTGTGTTCCTTATTCTCTCAAAATCTTCGATTTTGTTTTCGTTCGATCCCAATTTCGTATATGTTCTTTGGTTTTGTTATGGGTTTCTCGAGAAGAAGAAGTGTTAAGTCTAAAGTTAAAGAGTTTTAGAATTTTTGAAAGAGAGTTAAGAGAGATGGCACTAGTTCCATTTAAAGACACAAGGAATAAGAGAGTTTTAGAAGCTAAAACAAAAGCAAGCTAGGGTTAAAGCATATACAAGAAACCAAAUBQ10 promoter7,0207,0407,0607,0807,1007,1207,1407,160
    AGATTCTGTTAATCTTAGATCGAAGACGATTTTCTGGGTTTGATCGTTAGATATCATCTTAATTCTCGATTAGGGTTTCATAGATATCATCCGATTTGTTCAAATAATTTGAGTTTTGTCGAATAATTACTCTTCGATTTGTGATTTCTATCTAGATCTGGTGTTAGTTTCTAGTTCTAAGACAATTAGAATCTAGCTTCTGCTAAAAGACCCAAACTAGCAATCTATAGTAGAATTAAGAGCTAATCCCAAAGTATCTATAGTAGGCTAAACAAGTTTATTAAACTCAAAACAGCTTATTAATGAGAAGCTAAACACTAAAGATAGATCTAGACCACAATCAAAGATCAUBQ10 promoter7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TTGTGCGATCGAATTTGTCGATTAATCTGAGTTTTTCTGATTAACAGggattctagaactagtggatctacaaaatgtctagagccccaaagaagaagagaaaagttagacccggggataagaaatactctattggtttggctatcggtacaaactctgttggttgggctgttatAACACGCTAGCTTAAACAGCTAATTAGACTCAAAAAGACTAATTGTCcctaagatcttgatcacctagatgttttacagatctcggggtttcttcttctcttttcaatctgggcccctattctttatgagataaccaaaccgatagccatgtttgagacaaccaacccgacaataSmaISpeIXmaITspMIA1P2K3K4K5R6K7V8R97434-7460dCas9_VP64mdCas9UBQ10 promoterPSNLS7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tactgatgaatacaaggttccatccaagaagttcaaggttttgggtaacactgatagacactccatcaaaaagaacttgattggtgccttgttgttcgattctggtgaaactgctgaagctactagattgaaaagaaccgctagaagaagatacaccagaagaaagaacagaatcatgactacttatgttccaaggtaggttcttcaagttccaaaacccattgtgactatctgtgaggtagtttttcttgaactaaccacggaacaacaagctaagaccactttgacgacttcgatgatctaacttttcttggcgatcttcttctatgtggtcttctttcttgtcttagdCas9_VP64mdCas97,5407,5607,5807,6007,6207,6407,6607,6807,700
    tgctacttgcaagaaatcttctccaacgaaatggccaaggttgatgattcattcttccacagattggaagaatccttcttggtcgaagaagataagaagcacgaaagacatccaatcttcggtaacatcgttgatgaagttgcttaccacgaaaagtacccaactatctaccattacgatgaacgttctttagaagaggttgctttaccggttccaactactaagtaagaaggtgtctaaccttcttaggaagaaccagcttcttctattcttcgtgctttctgtaggttagaagccattgtagcaactacttcaacgaatggtgcttttcatgggttgatagatggtaadCas9_VP64mdCas97,7207,7407,7607,7807,8007,8207,8407,860
    tgagaaagaagttggttgactctaccgataaggctgatttgagattgatctatttggctttggcccacatgattaagttcagaggtcatttcttgatcgaaggtgatttgaacccagataactccgatgttgataagttgttcatccaattagtccaaacctacaatcaattattactctttcttcaaccaactgagatggctattccgactaaactctaactagataaaccgaaaccgggtgtactaattcaagtctccagtaaagaactagcttccactaaacttgggtctattgaggctacaactattcaacaagtaggttaatcaggtttggatgttagttaataadCas9_VP64mdCas97,8807,9007,9207,9407,9607,9808,0008,0208,040
    cgaagaaaacccaatcaacgcctctggtgttgatgctaaagctattttgtctgccagattgtccaagtccagaagattagaaaatttgatcgcccaattaccaggtgaaaagaagaatggtttgttcggtaatttgattgccttgtctttgggtttgactccaaacttcaagtccgcttcttttgggttagttgcggagaccacaactacgatttcgataaaacagacggtctaacaggttcaggtcttctaatcttttaaactagcgggttaatggtccacttttcttcttaccaaacaagccattaaactaacggaacagaaacccaaactgaggtttgaagttcaggdCas9_VP64mdCas98,0608,0808,1008,1208,1408,1608,1808,2008,220
    aatttcgatttggctgaagatgccaagttgcaattatctaaggatacctacgatgacgatttggataacttgttggctcaaatcggtgatcaatacgctgatttgtttttggctgctaagaacttgtccgatgccattttgttgtccgatattttgagagtcaacaccgaaattattaaagctaaaccgacttctacggttcaacgttaatagattcctatggatgctactgctaaacctattgaacaaccgagtttagccactagttatgcgactaaacaaaaaccgacgattcttgaacaggctacggtaaaacaacaggctataaaactctcagttgtggctttaatdCas9_VP64mdCas98,2408,2608,2808,3008,3208,3408,3608,3808,400
    ctaaggctccattgtctgcctctatgatcaaaagatacgatgaacaccaccaagacttgactttgttgaaggctttggtcagacaacaattacctgaaaagtacaaagaaattttcttcgatcaatccaagaacggttacgccggttatattgatggtggtgcttctcaagaagagattccgaggtaacagacggagatactagttttctatgctacttgtggtggttctgaactgaaacaacttccgaaaccagtctgttgttaatggacttttcatgtttctttaaaagaagctagttaggttcttgccaatgcggccaatataactaccaccacgaagagttcttctdCas9_VP64mdCas98,4208,4408,4608,4808,5008,5208,5408,560
    attttacaagttcatcaagccaatcttggaaaagatggacggtactgaagaattattggtcaagttgaacagagaagatttgttgagaaagcaaagaaccttcgacaacggttctattccacatcaaattcacttgggtgaattgcacgcaattttgagaagacaagaagatttttaaaatgttcaagtagttcggttagaaccttttctacctgccatgacttcttaataaccagttcaacttgtctcttctaaacaactctttcgtttcttggaagctgttgccaagataaggtgtagtttaagtgaacccacttaacgtgcgttaaaactcttctgttcttctaaaadCas9_VP64mdCas98,5808,6008,6208,6408,6608,6808,7008,7208,740
    tatccattcttgaaggacaacagagaaaagatcgaaaagattctgaccttcagaatcccttactacgttggtccattggctagaggtaattcaagatttgcctggatgactagaaagtccgaagaaactattactccttggaacttcgaagaagttgtagataagggtgcttctgataggtaagaacttcctgttgtctcttttctagcttttctaagactggaagtcttagggaatgatgcaaccaggtaaccgatctccattaagttctaaacggacctactgatctttcaggcttctttgataatgaggaaccttgaagcttcttcaacatctattcccacgaagacdCas9_VP64mdCas98,7608,7808,8008,8208,8408,8608,8808,9008,920
    cccaatcctttattgaaagaatgaccaacttcgacaagaacttgccaaacgaaaaggttttgccaaagcactctttgttgtacgaatacttcaccgtctacaacgaattgactaaggttaagtacgtcaccgaaggtatgagaaaaccagcttttttatccggtgaacaaaagaagggttaggaaataactttcttactggttgaagctgttcttgaacggtttgcttttccaaaacggtttcgtgagaaacaacatgcttatgaagtggcagatgttgcttaactgattccaattcatgcagtggcttccatactcttttggtcgaaaaaataggccacttgttttcttBarIdCas9_VP64mdCas98,9408,9608,9809,0009,0209,0409,0609,0809,100
    ggctatcgtcgatttgttgttcaagaccaacagaaaggttactgtcaagcaattaaaagaagattacttcaagaaaatcgaatgcttcgactccgttgaaatttctggtgtcgaagatagattcaatgcctctttaggtacttaccatgacttgttgaaaatcatcaaggacaagccgatagcagctaaacaacaagttctggttgtctttccaatgacagttcgttaattttcttctaatgaagttcttttagcttacgaagctgaggcaactttaaagaccacagcttctatctaagttacggagaaatccatgaatggtactgaacaacttttagtagttcctgttcdCas9_VP64mdCas99,1209,1409,1609,1809,2009,2209,2409,260
    gatttcttggacaacgaagaaaacgaagatattttggaagatattgtcttgacattgaccttgtttgaagatagagaaatgattgaagaaagattgaaaacctacgcccacttgttcgatgataaggttatgaagcaattaaagagaagaagatacactggttggggtagattgtctaaagaacctgttgcttcttttgcttctataaaaccttctataacagaactgtaactggaacaaacttctatctctttactaacttctttctaacttttggatgcgggtgaacaagctactattccaatacttcgttaatttctcttcttctatgtgaccaaccccatctaacadCas9_VP64mdCas99,2809,3009,3209,3409,3609,3809,4009,4209,440
    ccagaaaattgattaacggtatcagagacaagcaatccggtaagaccattttggactttttgaagtctgatggtttcgctaacagaaacttcatgcaattaatccacgacgattccttgactttcaaagaagatatacaaaaggcccaagtctctggtcaaggtgattctttacaggtcttttaactaattgccatagtctctgttcgttaggccattctggtaaaacctgaaaaacttcagactaccaaagcgattgtctttgaagtacgttaattaggtgctgctaaggaactgaaagtttcttctatatgttttccgggttcagagaccagttccactaagaaatgtdCas9_VP64mdCas99,4609,4809,5009,5209,5409,5609,5809,6009,620
    tgaacatatcgctaacttggctggttctccagctattaagaagggtattttacaaaccgttaaggtcgttgacgaattggtcaaagttatgggtagacataagccagaaaacatcgttatcgaaatggctagagaaaatcaaaccacccaaaagggtcaaaagaactccagagaaacttgtatagcgattgaaccgaccaagaggtcgataattcttcccataaaatgtttggcaattccagcaactgcttaaccagtttcaatacccatctgtattcggtcttttgtagcaatagctttaccgatctcttttagtttggtgggttttcccagttttcttgaggtctcttdCas9_VP64mdCas99,6409,6609,6809,7009,7209,7409,7609,7809,800
    agaatgaagagaatcgaagaaggtatcaaagaattgggttcccaaattttgaaagaacacccagttgaaaacacccaattacaaaacgaaaagttgtacttgtactacttgcaaaacggtagagatatgtacgttgaccaagaattggacatcaacagattgtctgattacgatgtcttacttctcttagcttcttccatagtttcttaacccaagggtttaaaactttcttgtgggtcaacttttgtgggttaatgttttgcttttcaacatgaacatgatgaacgttttgccatctctatacatgcaactggttcttaacctgtagttgtctaacagactaatgctacdCas9_VP64mdCas99,8209,8409,8609,8809,9009,9209,9409,960
    ttgacgctatcgttccacaatcttttttgaaggatgactccattgacaacaaggtcttgactagatccgataagaatagaggtaagtccgataacgttccatctgaagaagtcgttaagaaaatgaagaactattggagacaattattgaacgccaagttgatcacccaaagaaaaactgcgatagcaaggtgttagaaaaaacttcctactgaggtaactgttgttccagaactgatctaggctattcttatctccattcaggctattgcaaggtagacttcttcagcaattcttttacttcttgataacctctgttaataacttgcggttcaactagtgggtttctttdCas9_VP64mdCas99,98010,00010,02010,04010,06010,08010,10010,12010,140
    gtttgacaatttgaccaaggctgaaagaggtggtttgtctgaattggataaggcaggtttcatcaaaagacaattagtagaaaccagacaaatcaccaagcacgttgctcaaattttggatagtagaatgaacactaagtacgacgaaaacgacaaattgatcagagaagttaagcaaactgttaaactggttccgactttctccaccaaacagacttaacctattccgtccaaagtagttttctgttaatcatctttggtctgtttagtggttcgtgcaacgagtttaaaacctatcatcttacttgtgattcatgctgcttttgctgtttaactagtctcttcaattcdCas9_VP64mdCas910,16010,18010,20010,22010,24010,26010,28010,30010,320
    gtcattaccttgaagtccaagttggtttccgatttcagaaaggacttccaattctacaaggtcagagaaatcaacaactaccatcatgcacatgatgcttacttgaatgctgttgttggtactgccttgattaagaagtatccaaagttggaatccgaatttgtctacggtgattcagtaatggaacttcaggttcaaccaaaggctaaagtctttcctgaaggttaagatgttccagtctctttagttgttgatggtagtacgtgtactacgaatgaacttacgacaacaaccatgacggaactaattcttcataggtttcaaccttaggcttaaacagatgccactaadCas9_VP64mdCas910,34010,36010,38010,40010,42010,44010,46010,48010,500
    acaaggtttacgacgttagaaagatgatcgccaagtccgaacaagaaattggtaaagctactgccaaatacttcttctactccaatattatgaatttctttaagaccgaaatcactttggccaacggtgaaattagaaaaagaccattgattgaaactaatggtgaaacaggtgatgttccaaatgctgcaatctttctactagcggttcaggcttgttctttaaccatttcgatgacggtttatgaagaagatgaggttataatacttaaagaaattctggctttagtgaaaccggttgccactttaatctttttctggtaactaactttgattaccactttgtccactdCas9_VP64mdCas910,52010,54010,56010,58010,60010,62010,64010,660
    aatcgtttgggataagggtagagattttgccactgttagaaaggtattgtccatgccacaagtaaacatcgtcaaaaagaccgaagttcaaactggtggtttctccaaagaatccattttgcctaagagaaactccgataagttgatcgctagaaaaaaagactgggacccaaaattagcaaaccctattcccatctctaaaacggtgacaatctttccataacaggtacggtgttcatttgtagcagtttttctggcttcaagtttgaccaccaaagaggtttcttaggtaaaacggattctctttgaggctattcaactagcgatctttttttctgaccctgggttttKflIdCas9_VP64mdCas910,68010,70010,72010,74010,76010,78010,80010,82010,840
    aagtacggtggttttgattctccaactgttgcttactctgttttggttgttgctaaggtcgaaaagggtaagagtaagaagttgaagtccgtcaaagaattattaggtatcactatcatggaaagatcctcattcgaaaagaatcctatcgactttttggaagccaagggttacattcatgccaccaaaactaagaggttgacaacgaatgagacaaaaccaacaacgattccagcttttcccattctcattcttcaacttcaggcagtttcttaataatccatagtgatagtacctttctaggagtaagcttttcttaggatagctgaaaaaccttcggttcccaatgtdCas9_VP64mdCas910,86010,88010,90010,92010,94010,96010,98011,00011,020
    aagaagtcaagaaggacttgatcattaagttgccaaagtacagtttgttcgaattggaaaatggtagaaagagaatgttggcttctgccggtgaattacaaaagggtaatgaattggctttgccatccaagtacgttaatttcttatacttggcctcccactacgaaaaattgaattcttcagttcttcctgaactagtaattcaacggtttcatgtcaaacaagcttaaccttttaccatctttctcttacaaccgaagacggccacttaatgttttcccattacttaaccgaaacggtaggttcatgcaattaaagaatatgaaccggagggtgatgctttttaacttdCas9_VP64mdCas911,04011,06011,08011,10011,12011,14011,16011,18011,200
    aggttctcctgaagataacgaacaaaagcaattatttgtcgaacaacacaagcactacttggacgaaatcattgaacaaatttccgaattttccaaaagagtcattttggctgacgccaatttggacaaagttttgtcagcttacaacaagcacagagataagccaattagagaatccaagaggacttctattgcttgttttcgttaataaacagcttgttgtgttcgtgatgaacctgctttagtaacttgtttaaaggcttaaaaggttttctcagtaaaaccgactgcggttaaacctgtttcaaaacagtcgaatgttgttcgtgtctctattcggttaatctcttdCas9_VP64mdCas911,22011,24011,26011,28011,30011,32011,34011,360
    caagctgaaaacatcattcacttgttcactttgactaacttgggtgctccagctgcttttaagtatttcgataccactatcgacagaaagagatacacctctaccaaagaagttttggacgctactttgatccaccaatctattactggtttgtacgaaactagaatcgacttgtgttcgacttttgtagtaagtgaacaagtgaaactgattgaacccacgaggtcgacgaaaattcataaagctatggtgatagctgtctttctctatgtggagatggtttcttcaaaacctgcgatgaaactaggtggttagataatgaccaaacatgctttgatcttagctgaacadCas9_VP64mdCas911,38011,40011,42011,44011,46011,48011,50011,52011,540
    ctcaattaggtggtgatGGTTCTGGATCAGGTAGTGGCTCAGGATCTaTGAAGAGAGATCATCACCAATTCCAAGGTCGATTGTCCAACCACGGGACTTCTTCTTCATCATCATCAATCTCTAAAGATAAGATGATGATGGTGaaaaaaGAAGAAGACGGTGGAGGTAACATGGAgagttaatccaccactaCCAAGACCTAGTCCATCACCGAGTCCTAGAtACTTCTCTCTAGTAGTGGTTAAGGTTCCAGCTAACAGGTTGGTGCCCTGAAGAAGAAGTAGTAGTAGTTAGAGATTTCTATTCTACTACTACCACttttttCTTCTTCTGCCACCTCCATTGTACCTMK2RD4HH6QF8QG10RL12SN14HG16TS18SS20SS22SI24SK26DK28MM30MV32KK34EE36DG38GG40NM42D11598-13358RGA1L1dCas9_VP64mdCas911,56011,58011,60011,62011,64011,66011,68011,70011,720
    CGACGAGCTTCTCGCTGTTTTAGGTTACAAAGTTAGGTCATCGGAGATGGCGGAGGTTGCTTTGAAACTCGAACAATTAGAGACGATGATGAGTAATGTTCAAGAAGATGGTTTATCTCATCTCGCGACGGATACTGTTCATTATAATCCGTCGGAGCTTTATTCTTGGCTTGATGCTGCTCGAAGAGCGACAAAATCCAATGTTTCAATCCAGTAGCCTCTACCGCCTCCAACGAAACTTTGAGCTTGTTAATCTCTGCTACTACTCATTACAAGTTCTTCTACCAAATAGAGTAGAGCGCTGCCTATGACAAGTAATATTAGGCAGCCTCGAAATAAGAACCGAACTANruID44EL46LA48VL50GY52KV54RS56SE58MA60EV62AL64KL66EQ68LE70TM72MS74NV76QE78DG80LS82HL84AT86DT88VH90YN92PS94EL96YS98WL100D11598-13358RGA1GA Degron RGA longerGA 'degron' RGA11,74011,76011,78011,80011,82011,84011,86011,88011,900
    AATATGCTCTCTGAGCTTAATCCTCCTCCTCTTCCGGCGAGTTCTAACGGTTTAGATCCGGTTCTTCCTTCGCCGGAGATTTGTGGTTTTCCGGCTTCGGATTATGACCTTAAAGTCATTCCCGGAAACGCGATTTATCAGTTTCCGGCGATTGATTCTTCGTCTTCGTCGAATATTATACGAGAGACTCGAATTAGGAGGAGGAGAAGGCCGCTCAAGATTGCCAAATCTAGGCCAAGAAGGAAGCGGCCTCTAAACACCAAAAGGCCGAAGCCTAATACTGGAATTTCAGTAAGGGCCTTTGCGCTAAATAGTCAAAGGCCGCTAACTAAGAAGCAGAAGCAGCTTATN102ML104SE106LN108PP110PL112PA114SS116NG118LD120PV122LP124SP126EI128CG130FP132AS134DY136DL138KV140IP142GN144AI146YQ148FP150AI152DS154SS156SS158N11598-13358RGA1GA Degron RGA longer11,92011,94011,96011,98012,00012,02012,04012,060
    ATCAGAACAAGCGTTTGAAATCATGCTCGAGTCCTGATTCTATGGTTACATCGACTTCGACGGGTACGCAGATTGGTGGAGTCATAGGAACGACGGTGACGACAACCACCACGACAACGACGGCGGCGGGTGAGTCAACTCGTTCTGTTATCCTGGTTGACTCGCAAGAGAACGGTAGTCTTGTTCGCAAACTTTAGTACGAGCTCAGGACTAAGATACCAATGTAGCTGAAGCTGCCCATGCGTCTAACCACCTCAGTATCCTTGCTGCCACTGCTGTTGGTGGTGCTGTTGCTGCCGCCGCCCACTCAGTTGAGCAAGACAATAGGACCAACTGAGCGTTCTCTTGCCPspXIN160QN162KR164LK166SC168SS170PD172SM174VT176ST178ST180GT182QI184GG186VI188GT190TV192TT194TT196TT198TT200AA202GE204ST206RS208VI210LV212DS214QE216NG21811598-13358RGA112,08012,10012,12012,14012,16012,18012,20012,22012,240
    TGTTCGTTTAGTCCACGCGCTTATGGCTTGTGCAGAAGCAATCCAGCAGAACAATTTGACTCTAGCGGAAGCTCTTGTGAAGCAAATCGGATGCTTAGCTGTGTCTCAAGCCGGAGCTATGAGAAAAGTGGCTACTTACTTCGCCGAAGCTTTAGCGCGGCGGATCTACCGTCTCACAAGCAAATCAGGTGCGCGAATACCGAACACGTCTTCGTTAGGTCGTCTTGTTAAACTGAGATCGCCTTCGAGAACACTTCGTTTAGCCTACGAATCGACACAGAGTTCGGCCTCGATACTCTTTTCACCGATGAATGAAGCGGCTTCGAAATCGCGCCGCCTAGATGGCAGAGVR220LV222HA224LM226AC228AE230AI232QQ234NN236LT238LA240EA242LV244KQ246IG248CL250AV252SQ254AG256AM258RK260VA262TY264FA266EA268LA270RR272IY274RL27611598-13358RGA112,26012,28012,30012,32012,34012,36012,38012,40012,420
    TCTCCGCCGCAGAATCAGATCGATCATTGTCTCTCCGATACTCTTCAGATGCACTTTTACGAGACTTGTCCTTATCTTAAATTCGCTCACTTCACGGCGAACCAAGCGATTCTCGAAGCTTTTGAAGGTAAGAAGAGAGTACACGTCATTGATTTCTCGATGAACCAAGGTCTTCAGAGGCGGCGTCTTAGTCTAGCTAGTAACAGAGAGGCTATGAGAAGTCTACGTGAAAATGCTCTGAACAGGAATAGAATTTAAGCGAGTGAAGTGCCGCTTGGTTCGCTAAGAGCTTCGAAAACTTCCATTCTTCTCTCATGTGCAGTAACTAAAGAGCTACTTGGTTCCAGAAGSP278PQ280NQ282ID284HC286LS288DT290LQ292MH294FY296ET298CP300YL302KF304AH306FT308AN310QA312IL314EA316FE318GK320KR322VH324VI326DF328SM330NQ332GL33411598-13358RGA112,44012,46012,48012,50012,52012,54012,56012,58012,600
    AATGGCCTGCACTTATGCAAGCTCTTGCGCTTCGAGAAGGAGGTCCTCCAACTTTCCGGTTAACCGGAATTGGTCCACCGGCGCCGGATAATTCTGATCATCTTCATGAAGTTGGTTGTAAATTAGCTCAGCTTGCGGAGGCGATTCACGTAGAATTCGAATACCGTGGATTCGTTTACCGGACGTGAATACGTTCGAGAACGCGAAGCTCTTCCTCCAGGAGGTTGAAAGGCCAATTGGCCTTAACCAGGTGGCCGCGGCCTATTAAGACTAGTAGAAGTACTTCAACCAACATTTAATCGAGTCGAACGCCTCCGCTAAGTGCATCTTAAGCTTATGGCACCTAAGCAHpaIEcoRIQW336PA338LM340QA342LA344LR346EG348GP350PT352FR354LT356GI358GP360PA362PD364NS366DH368LH370EV372GC374KL376AQ378LA380EA382IH384VE386FE388YR390GF392V11598-13358RGA112,62012,64012,66012,68012,70012,72012,74012,760
    TGCTAACAGCTTAGCCGATCTCGATGCTTCGATGCTTGAGCTTAGACCGAGCGATACGGAAGCTGTTGCGGTGAACTCTGtttttGAGCTACATAAGCTCTTAGGTCGTCCCGGTGGGATAGAGAAAGTTCTCGGCGTTGTGAAACAGATTAAACCGGTGATTTTCACGGTGGTTACGATTGTCGAATCGGCTAGAGCTACGAAGCTACGAACTCGAATCTGGCTCGCTATGCCTTCGACAACGCCACTTGAGACaaaaaCTCGATGTATTCGAGAATCCAGCAGGGCCACCCTATCTCTTTCAAGAGCCGCAACACTTTGTCTAATTTGGCCACTAAAAGTGCCACCAAA394NS396LA398DL400DA402SM404LE406LR408PS410DT412EA414VA416VN418SV420FE422LH424KL426LG428RP430GG432IE434KV436LG438VV440KQ442IK444PV446IF448TV450V11598-13358RGA112,78012,80012,82012,84012,86012,88012,90012,92012,940
    GAGCAAGAATCGAACCATAACGGACCGGTTTTCTTAGACCGGTTTACTGAATCGTTACATTATTATTCGACTCTGTTTGATTCGTTGGAAGGAGTTCCGAATAGTCAAGACAAAGTCATGTCTGAAGTTTACTTAGGGAAACAGATTTGTAATCTGGTGGCTTGTGAAGGTCCTGCTCGTTCTTAGCTTGGTATTGCCTGGCCAAAAGAATCTGGCCAAATGACTTAGCAATGTAATAATAAGCTGAGACAAACTAAGCAACCTTCCTCAAGGCTTATCAGTTCTGTTTCAGTACAGACTTCAAATGAATCCCTTTGTCTAAACATTAGACCACCGAACACTTCCAGGACRsrIIE452QE454SN456HN458GP460VF462LD464RF466TE468SL470HY472YS474TL476FD478SL480EG482VP484NS486QD488KV490MS492EV494YL496GK498QI500CN502LV504AC506EG508P11598-13358RGA112,96012,98013,00013,02013,04013,06013,08013,10013,120
    ACAGAGTCGAGAGACACGAAACGTTGAGTCAATGGGGAAACCGGTTTGGTTCGTCCGGTTTAGCGCCGGCACATCTTGGGTCTAACGCGTTTAAGCAAGCGAGTATGCTTTTGTCTGTGTTTAATAGTGGCCAAGGTTATCGTGTGGAGGAGAGTAATGGATGTTTGATGTTGGGTGTCTCAGCTCTCTGTGCTTTGCAACTCAGTTACCCCTTTGGCCAAACCAAGCAGGCCAAATCGCGGCCGTGTAGAACCCAGATTGCGCAAATTCGTTCGCTCATACGAAAACAGACACAAATTATCACCGGTTCCAATAGCACACCTCCTCTCATTACCTACAAACTACAACCCD510RV512ER514HE516TL518SQ520WG522NR524FG526SS528GL530AP532AH534LG536SN538AF540KQ542AS544ML546LS548VF550NS552GQ554GY556RV558EE560SN562GC564LM566LG56811598-13358RGA113,14013,16013,18013,20013,22013,24013,26013,28013,300
    TTGGCACACTCGTCCACTCATTACCACCTCCGCTTGGAAACTCTCGACGGCGGCGTACGctgcaggaggcggtggaagcATGTCTTCTCTTAGTAGAGAGCTCGTTTTCTTGATCTTACAGTTTCTCGATGAAGAGAAGTTTAAAGAGACTGTTCACAAGCTTGAACAAGAATCTAACCGTGTGAGCAGGTGAGTAATGGTGGAGGCGAACCTTTGAGAGCTGCCGCCGCATGCgacgtcctccgccaccttcgTACAGAAGAGAATCATCTCTCGAGCAAAAGAACTAGAATGTCAAAGAGCTACTTCTCTTCAAATTTCTCTGACAAGTGTTCGAACTTGTTCTTAGABsiWIWH570TR572PL574IT576TS578AW580KL582ST584AA586Y11598-13358TPLRD2RGA1F primerL2F primer13,32013,34013,36013,38013,40013,42013,44013,460
    GGGTTTTTCTTCAATATGAAGTATTTTGAGGATGAGGTTCACAATGGAAATTGGGATGAGGTTGAGAAGTATCTCTCTGGTTTCACTAAAGTAGATGATAATAGATACTCTATGAAGATATTCTTCGAGATAAGAAAGCAGAAGTATCTCGAGGCTTTGGATAAGCATGATCGTCCCCAAAAAGAAGTTATACTTCATAAAACTCCTACTCCAAGTGTTACCTTTAACCCTACTCCAACTCTTCATAGAGAGACCAAAGTGATTTCATCTACTATTATCTATGAGATACTTCTATAAGAAGCTCTATTCTTTCGTCTTCATAGAGCTCCGAAACCTATTCGTACTAGCAGTPLRD2CTLH domain13,48013,50013,52013,54013,56013,58013,60013,62013,640
    CCAAGGCTGTGGATATACTAGTGAAGGATTTGAAAGTGTTTTCAACTTTTAATGAGGAGCTTTTCAAGGAAATAACACAGCTCTTGACATTGGAGAACTTCCGGGAGAATGAACAGTTATCCAAGTATGGGGACACCAAGTCTGCAAGAGCGATCATGTTGGTGGAACTCAAAAAGGTTCCGACACCTATATGATCACTTCCTAAACTTTCACAAAAGTTGAAAATTACTCCTCGAAAAGTTCCTTTATTGTGTCGAGAACTGTAACCTCTTGAAGGCCCTCTTACTTGTCAATAGGTTCATACCCCTGTGGTTCAGACGTTCTCGCTAGTACAACCACCTTGAGTTTTTSpeITPLRD213,66013,68013,70013,72013,74013,76013,78013,80013,820
    GCTGATTGAGGCGAATCCTTTATTCCGTGATAAATTGCAGTTCCCTACTCTTAGAAATTCAAGGCTGAGGACTTTGATCAACCAGAGCTTAAATTGGCAACACCAGCTTTGTAAAAATCCAAGGCCAAATCCAGATATCAAGACTCTTTTTGTGGATCACTCTTGTGGCCCTCCACGACTAACTCCGCTTAGGAAATAAGGCACTATTTAACGTCAAGGGATGAGAATCTTTAAGTTCCGACTCCTGAAACTAGTTGGTCTCGAATTTAACCGTTGTGGTCGAAACATTTTTAGGTTCCGGTTTAGGTCTATAGTTCTGAGAAAAACACCTAGTGAGAACACCGGGAGGTBbvCITPLRD2Pro-rich13,84013,86013,88013,90013,92013,94013,96013,98014,000
    AATGGCGCACGAGCTCCATCTCCTGTGAACAATCCACTGCTTGGAGGGATACCAAAGGCTGGAGGATTTCCTCCTTTAGGCGCACACGGGCCATTTCAACCAACGGCGTCACCTGTTCCAACACCTCTTGCTGGTTGGATGTCTAGTCCATCCTCTGTCCCACATCCAGCTGTCTTTACCGCGTGCTCGAGGTAGAGGACACTTGTTAGGTGACGAACCTCCCTATGGTTTCCGACCTCCTAAAGGAGGAAATCCGCGTGTGCCCGGTAAAGTTGGTTGCCGCAGTGGACAAGGTTGTGGAGAACGACCAACCTACAGATCAGGTAGGAGACAGGGTGTAGGTCGACAGATPLRD2Pro-rich14,02014,04014,06014,08014,10014,12014,14014,160
    CAGCAGGAGCCATTGCACTTGGTGGTCCATCAATCCCAGCGGCGTTGAAGCACCCGAGAACTCCTCCAACTAATGCTTCTTTAGACTACCCGTCAGCAGATTCTtagCACGTGcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgatGTCGTCCTCGGTAACGTGAACCACCAGGTAGTTAGGGTCGCCGCAACTTCGTGGGCTCTTGAGGAGGTTGATTACGAAGAAATCTGATGGGCAGTCGTCTAAGAatcGTGCACgcaagtttgtaaaccgttatttcaaagaattctaacttaggacaacggccagaacgctactaDraIIITPLRD2Pro-richnos (nopaline synthase) 3'UTR (polyA signal)14,18014,20014,22014,24014,26014,28014,30014,32014,340
    tatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatagtatattaaagacaacttaatgcaattcgtacattattaattgtacattacgtactgcaataaatactctacccaaaaatactaatctcagggcgttaatatgtaaattatgcgctatcttttgttttatatcgcgcgtttgatcctatttaatagcgcgcgccacagtagaMauBInos (nopaline synthase) 3'UTR (polyA signal)14,36014,38014,40014,42014,44014,46014,48014,50014,520
    atgttactagatcgggCCTGCAGGACGCGTGGCGCGCCagatccAATTAAACTATCAGTGTTTGACAGGATATATTGGcgggtaaacctaagagaaaagagcgtttattagaataacggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccatacaatgatctagcccGGACGTCCTGCGCACCGCGCGGtctaggTTAATTTGATAGTCACAAACTGTCCTATATAACCgcccatttggattctcttttctcgcaaataatcttattgcctataaattttcccgcacttttccaaataggcaagcaggtaaacatacacgtacggtAscISbfIright bord...NA repeatRareCut-...scISbfInos (...nal)14,54014,56014,58014,60014,62014,64014,66014,68014,700
    accacagggttcccctcgggatcaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttggtgtcccaaggggagccctagtttcatgaaactaggttggggaggcgacgatatcacgtcagccgaagactgcaagtcacgtcggcagaagacttttgctgtacagcgtgttcaggattcaatgcgctgtccgacggcgggacgggaaaaggaccgcaaaagaacagcgcaca14,72014,74014,76014,78014,80014,82014,84014,860
    tttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagaaaatcagcgtatttcatcttatgaacgctgatcttggcctctgtaatgcggtacttgttctcgcggcggcgaccggacgacccgatacgggcgcagtcgtggctgctggtcctgaactggttggttgcccggcttgacgtgcgccggccgacgtggttcgacaaaaggctcttct14,88014,90014,92014,94014,96014,98015,00015,02015,040
    tcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgacctactggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccagtggccgtggtccgcgctggcgggcctcgaccggtcctacgaactggtggatgcgggaccgctgcaacactgtcactggtccgatctggcggaccgggcgtcgtgggcgctggatgacctgtaacggctcgcgtaggtcctccggccgcgcccggacgcatcggaccgtctcgg15,06015,08015,10015,12015,14015,16015,18015,20015,220
    gtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcaccacccggctgtggtggtgcggccggccggcgtaccacaactggcacaagcggccgtaacggctcaagctcgcaagggattagtagctggcgtgggcctcgcccgcgctccggcggttccgggctccgcacttcaaaccgggggcgggatgggagtggggccgtgtctagcgcgtg15,24015,26015,28015,30015,32015,34015,36015,38015,400
    gcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgggcgctcgactagctggtccttccggcgtggcactttctccgccgacgtgacgaaccgcacgtagcgagctgggacatggcgcgtgaactcgcgtcgctccttcactgcgggtggctccggtccgccgcgccacggaaggcactcctgcgtaactggctccggctgcgggacc15,42015,44015,46015,48015,50015,52015,54015,560
    cggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttggccggcggctcttacttgcggttctccttgttcgtactttggcgtggtcctgccggtcctgcttggcaaaaagtaatggcttctctagctccgcctctactagcgccggcccatgcacaagctcggcgggcgcgtgcagagttggcacgccgacgtactttaggaccggccaaacSTA region from pVS1 plasmid15,58015,60015,62015,64015,66015,68015,70015,72015,740
    tctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgcagactacggttcgaccgccggaccggccggtcgaaccggcgacttctttggctcgcggcggcagatttttccactacacataaactcattttgtcgaacgcagtacgccagcgacgcatatactacgctactcatttatttgtttatgcgttccccttgcgtacttccaatagcgSTA region from pVS1 plasmid15,76015,78015,80015,82015,84015,86015,88015,90015,920
    tgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcacatgaattggtctttccgcccagtccgttctgctggtagcgttgggtagatcgggcgcgggacgttgagcggccccggctacaagacaatcagctaaggctaggggtcccgtcacgggcgctaacccgccggcacgcccttctagttggcgattggcaacagccgtagctggcgSTA region from pVS1 plasmid15,94015,96015,98016,00016,02016,04016,06016,08016,100
    ccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggggctgctaactggcgctgcacttccggtagccggccgcgctgaagcatcactagctgcctcgcggggtccgccgcctgaaccgacacaggcgctagttccgtcggctgaagcacgactaaggccacgtcggttcgggaatgctgtatacccggtggcggctggaccacctcgaccSTA region from pVS1 plasmid16,12016,14016,16016,18016,20016,22016,24016,260
    ttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgcaattcgtcgcgtaactccagtgcctaccttccgatgttcgccggaaacagcacagcgcccgctagtttccgtgcgcgtagccgccactccaacggctccgcgaccggcccatgctcgacgggtaagaactcagggcatagtgcgtcgcgcactcgatgggtccgtgacggcggcgSTA region from pVS1 plasmid16,28016,30016,32016,34016,36016,38016,40016,42016,440
    cggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaagccgtgttggcaagaacttagtcttgggctcccgctgcgacgggcgctccaggtccgcgaccggcgactttaatttagttttgagtaaactcaattactccatttctcttttactcgttttcgtgtttgtgcgattcacggccggcaggctcgcgtgcgtcgtcgttccgacgttSTA region from pVS1 plasmid16,46016,48016,50016,52016,54016,56016,58016,60016,620
    cgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagcaaccggtcggaccgtctgtgcggtcggtacttcgcccagttgaaagtcaacggccgcctcctagtgtggttcgacttctacatgcgccatgcggttccgttctggtaatggctcgacgatagacttatgtagcgcgtcgatggtctcatttactcgtttacttatttactcatSTA region from pVS1 plasmid16,64016,66016,68016,70016,72016,74016,76016,78016,800
    gatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgacgctacttaaaatcgccgatttcctccgccgtaccttttagttcttgttggtccgtggctgcggcaccttacggggtacacacctccttgcccgccaaccggtccgcattcgccgacccaacagacggccgggacgttaccgtgaccttgggggttcgggctccttagccgcactgc16,82016,84016,86016,88016,90016,92016,94016,960
    gtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggccagcgtttggtaggccgggccatgtttagccgcgccgcgacccactactggaccacctcttcaacttccggcgcgtccggcgggtcgccgttgcgtagctccgtcttcgtgcggggccacttagcaccgttcgccggcgactagcttaggcgtttcttagggccgttggcggccg16,98017,00017,02017,04017,06017,08017,10017,12017,140
    agccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccatcggccacgcggcagctaatccttcggcgggttcccgctgctcgttggtctaaaaaagcaaggctacgagatactgcacccgtgggcgctatcagcgtcgtagtacctgcaccggcaaaaggcagacagcttcgcactggctgctcgaccgctccactaggcgatgctcgaaggtpVS1-REP; replication origin from pVS117,16017,18017,20017,22017,24017,26017,28017,30017,320
    gacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctctgcccgtgcatctccaaaggcgtcccggccggccgtaccggtcacacaccctaatgctggaccatgactaccgccaaagggtagattggcttaggtacttggctatggcccttcccttccctctgttcgggccggcgcacaaggcaggtgtgcaacgcctgcatgagttcaagapVS1-REP; replication origin from pVS117,34017,36017,38017,40017,42017,44017,46017,48017,500
    gccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccggcggccgctcggctaccgcctttcgtctttctgctggaccatctttggacgtaagccaatttgtggtgcgtgcaacggtacgtcgcatgcttcttccggttcttgccggcggaccactgccataggctcccacttcggaactaatcggcgatgttctagcatttctcgctttggccBsiWIpVS1-REP; replication origin from pVS117,52017,54017,56017,58017,60017,62017,64017,660
    gcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggcgccggcctcatgtagctctagctcgatcgactaacctacatggcgctctagtgtcttccgttcttgggcctgcacgactgccaagtggggctaatgaaaaactagctagggccgtagccggcaaaagagatggcggaccgtgcggcgcggcgtccgttccgtcttcggtctaccBmtINheIpVS1-REP; replication origin from pVS117,68017,70017,72017,74017,76017,78017,80017,82017,840
    ttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagaacaagttctgctagatgcttgcgtcaccgtcgcggcctctcaagttcttcaagacaaagtggcacgcgttcgactagcccagtttactggacggcctcatgctaaacttcctcctccgccccgtccgaccgggctaggatcagtacgcgatggcgttggactagctcccgcttcFspIpVS1-REP; replication origin from pVS117,86017,88017,90017,92017,94017,96017,98018,00018,020
    catccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattgggaacccaaagccgtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcgtaggcggccaaggattacatgcctcgtctacgatcccgtttaacgggatcgtcccctttttccagcttttccagagaaaggacacctatcgtgcatgtaacccttgggtttcggcatgtaacccttggccttgggcatgtaacccttgggtttcggcatgtaacccttggccagBsaIpVS1-REP; replication origin from pVS118,04018,06018,08018,10018,12018,14018,16018,18018,200
    acacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgcccctgtgtacattcactgactatattttctcttttttccgctaaaaaggcggattttgagaaattttgaataattttgagaattttgggcggaccggacacgtattgacagaccggtcgcgtgtcggcttctcgacgtttttcgcggatgggaagccagcgacgcgagggatgcggggpVS1-REP; replication origin from pVS118,22018,24018,26018,28018,30018,32018,34018,360
    gccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcggcgaagcgcagccggatagcgccggcgaccggcgagtttttaccgaccggatgccggtccgttagatggtcccgcgcctgttcggcgcggcagcggtgagctggcggccgcgggtgtagttccgtgggacggagcgcgcaaagccactactgccacttttggagactgtgtacMreI18,38018,40018,42018,44018,46018,48018,50018,52018,540
    cagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgaggtcgagggcctctgccagtgtcgaacagacattcgcctacggccctcgtctgttcgggcagtcccgcgcagtcgcccacaaccgcccacagccccgcgtcggtactgggtcagtgcatcgctatcgcctcacatatgaccgaattgatacgccgtagtctcgtctaacatgactcBstZ17Ibom site from pBR32218,56018,58018,60018,62018,64018,66018,68018,70018,720
    agtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataactcacgtggtatacgccacactttatggcgtgtctacgcattcctcttttatggcgtagtccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgbom site from pBR32218,74018,76018,78018,80018,82018,84018,86018,88018,900
    gcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggacbom site from pBR32218,92018,94018,96018,98019,00019,02019,04019,060
    gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgacttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctpBR322 origin of replication19,08019,10019,12019,14019,16019,18019,20019,22019,240
    ccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccapBR322 origin of replication19,26019,28019,30019,32019,34019,36019,38019,40019,420
    atctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagaattgcgaccgtcgaggagaacttctagtatatctatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttcttaacgctggcagctcctcttgaagatcatatagatLEU2 Promoter19,44019,46019,48019,50019,52019,54019,56019,58019,600
    catacctaatattattgccttattaaaaatggaatcccaacaattacatcaaaatccacattctcttcaaaatcaattgtcctgtacttccttgttcatgtgtgttcaaaaacgttatatttataggataattatactctatttctcaacaagtaattggttgtttggccgagcggtatggattataataacggaataatttttaccttagggttgttaatgtagttttaggtgtaagagaagttttagttaacaggacatgaaggaacaagtacacacaagtttttgcaatataaatatcctattaatatgagataaagagttgttcattaaccaacaaaccggctcgcLEU2 Promoter19,62019,64019,66019,68019,70019,72019,74019,760
    gtctaaggcgcctgattcaagaaatatcttgaccgcagttaactgtgggaatactcaggtatcgtaagatgcaagagttcgaatctcttagcaaccattatttttttcctcaacataacgagaacacacaggggcgctatcgcacagaatcaaattcgatgactggaaattttttcagattccgcggactaagttctttatagaactggcgtcaattgacacccttatgagtccatagcattctacgttctcaagcttagagaatcgttggtaataaaaaaaggagttgtattgctcttgtgtgtccccgcgatagcgtgtcttagtttaagctactgacctttaaaaaaHpaILEU2 Promoter19,78019,80019,82019,84019,86019,88019,90019,92019,940
    gttaatttcagaggtcgcctgacgcatatacctttttcaactgaaaaattgggagaaaaaggaaaggtgagagcgccggaaccggcttttcatatagaatagagaagcgttcatgactaaatgcttgcatcacaatacttgaagttgacaatattatttaaggacctattgttttcaattaaagtctccagcggactgcgtatatggaaaaagttgactttttaaccctctttttcctttccactctcgcggccttggccgaaaagtatatcttatctcttcgcaagtactgatttacgaacgtagtgttatgaacttcaactgttataataaattcctggataacaaaaLEU2 Promoter19,96019,98020,00020,02020,04020,06020,08020,10020,120
    ttccaataggtggttagcaatcgtcttactttctaacttttcttaccttttacatttcagcaatatatatatatatatttcaaggatataccattctaatgtctgcccctaagaagatcgtcgttttgccaggtgaccacgttggtcaagaaatcacagccgaagccattaaggtaaggttatccaccaatcgttagcagaatgaaagattgaaaagaatggaaaatgtaaagtcgttatatatatatatataaagttcctatatggtaagattacagacggggattcttctagcagcaaaacggtccactggtgcaaccagttctttagtgtcggcttcggtaattccaLEU2 PromoterLEU220,14020,16020,18020,20020,22020,24020,26020,28020,300
    tcttaaagctatttctgatgttcgttccaatgtcaagttcgatttcgaaaatcatttaattggtggtgctgctatcgatgctacaggtgttccacttccagatgaggcgctggaagcctccaagaaggctgatgccgttttgttaggtgctgtgggtggtcctaaatggggaaccagaatttcgataaagactacaagcaaggttacagttcaagctaaagcttttagtaaattaaccaccacgacgatagctacgatgtccacaaggtgaaggtctactccgcgaccttcggaggttcttccgactacggcaaaacaatccacgacacccaccaggatttaccccttggLEU220,32020,34020,36020,38020,40020,42020,44020,460
    ggtagtgttagacctgaacaaggtttactaaaaatccgtaaagaacttcaattgtacgccaacttaagaccatgtaactttgcatccgactctcttttagacttatctccaatcaagccacaatttgctaaaggtactgacttcgttgttgtcagagaattagtgggaggtatttccatcacaatctggacttgttccaaatgatttttaggcatttcttgaagttaacatgcggttgaattctggtacattgaaacgtaggctgagagaaaatctgaatagaggttagttcggtgttaaacgatttccatgactgaagcaacaacagtctcttaatcaccctccataaaLEU220,48020,50020,52020,54020,56020,58020,60020,62020,640
    actttggtaagagaaaggaagacgatggtgatggtgtcgcttgggatagtgaacaatacaccgttccagaagtgcaaagaatcacaagaatggccgctttcatggccctacaacatgagccaccattgcctatttggtccttggataaagctaatgttttggcctcttcaagatttgaaaccattctctttccttctgctaccactaccacagcgaaccctatcacttgttatgtggcaaggtcttcacgtttcttagtgttcttaccggcgaaagtaccgggatgttgtactcggtggtaacggataaaccaggaacctatttcgattacaaaaccggagaagttctaaLEU220,66020,68020,70020,72020,74020,76020,78020,80020,820
    atggagaaaaactgtggaggaaaccatcaagaacgaatttcctacattgaaggttcaacatcaattgattgattctgccgccatgatcctagttaagaacccaacccacctaaatggtattataatcaccagcaacatgtttggtgatatcatctccgatgaagcctccgttatctacctctttttgacacctcctttggtagttcttgcttaaaggatgtaacttccaagttgtagttaactaactaagacggcggtactaggatcaattcttgggttgggtggatttaccataatattagtggtcgttgtacaaaccactatagtagaggctacttcggaggcaatagLEU220,84020,86020,88020,90020,92020,94020,96020,98021,000
    ccaggttccttgggtttgttgccatctgcgtccttggcctctttgccagacaagaacaccgcatttggtttgtacgaaccatgccacggttctgctccagatttgccaaagaataaggtcaaccctatcgccactatcttgtctgctgcaatgatgttgaaattgtcattgaactggtccaaggaacccaaacaacggtagacgcaggaaccggagaaacggtctgttcttgtggcgtaaaccaaacatgcttggtacggtgccaagacgaggtctaaacggtttcttattccagttgggatagcggtgatagaacagacgacgttactacaactttaacagtaacttgaLEU221,02021,04021,06021,08021,10021,12021,14021,160
    tgcctgaagaaggtaaggccattgaagatgcagttaaaaaggttttggatgcaggtatcagaactggtgatttaggtggttccaacagtaccaccgaagtcggtgatgctgtcgccgaagaagttaagaaaatccttgcttaaaaagattctctttttttatgatatttgtacatacggacttcttccattccggtaacttctacgtcaatttttccaaaacctacgtccatagtcttgaccactaaatccaccaaggttgtcatggtggcttcagccactacgacagcggcttcttcaattcttttaggaacgaatttttctaagagaaaaaaatactataaacatgtaLEU2LEU2 Terminator21,18021,20021,22021,24021,26021,28021,30021,32021,340
    aaactttataaatgaaattcataatagaaacgacacgaaattacaaaatggaatatgttcatagggtagacgaaactatatacgcaatctacatacatttatcaagaaggagaaaaaggaggatgtaaaggaatacaggtaagcaaattgatactaatggctcaacgtgataaggtttgaaatatttactttaagtattatctttgctgtgctttaatgttttaccttatacaagtatcccatctgctttgatatatgcgttagatgtatgtaaatagttcttcctctttttcctcctacatttccttatgtccattcgtttaactatgattaccgagttgcactattccLEU2 Terminator21,36021,38021,40021,42021,44021,46021,48021,50021,520
    aaaaagaattgcactttaacattaatattgacaaggaggagggcaccacacaaaaagttaggtgtaacagaaaatcatgaaactatgattcctaatttatatattggaggattttctctaaaaaaaaaaaaatacaacaaataaaaaacactcaatgacctgaccatttgatggatttttcttaacgtgaaattgtaattataactgttcctcctcccgtggtgtgtttttcaatccacattgtcttttagtactttgatactaaggattaaatatataacctcctaaaagagatttttttttttttatgttgtttattttttgtgagttactggactggtaaactacctLEU2 Terminator21,54021,56021,58021,60021,62021,64021,66021,68021,700
    gtttaagtcaataccttcttgaaccatttcccataatggtgaaagttccctcaagaattttactctgtcagaaacggccttaacgacgtagtcgatgacggatcgcttgcctgtaacttacacgcgcctcgtatcttttaatgatggaataatttgggaatttactctgtgtttacaaattcagttatggaagaacttggtaaagggtattaccactttcaagggagttcttaaaatgagacagtctttgccggaattgctgcatcagctactgcctagcgaacggacattgaatgtgcgcggagcatagaaaattactaccttattaaacccttaaatgagacacaaatLEU2 TerminatorARS421,72021,74021,76021,78021,80021,82021,84021,860
    tttatttttatgttttgtatttggattttagaaagtaaataaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgtactttacatatatatttattagacaagaaaagcagattaaatagatatacattcgattaacgaaataaaaatacaaaacataaacctaaaatctttcatttatttcttccatcttctcaatgccttacttctttttttttatttgtttccaaattttttaaagttgtttttcgcatgaaatgtatatataaataatctgttcttttcgtctaatttatctatatgtaagctaattgcARS421,88021,90021,92021,94021,96021,98022,00022,02022,040
    ataagtaaaatgtaaaatcacaggattttcgtgtgtggtcttctacacagacaagatgaaacaattcggcattaatacctgagagcaggaagagcaagataaaaggtagtatttgttggcgatccccctagagtcttttacatcttcggaaaacaaaaactattttttctttaattattcattttacattttagtgtcctaaaagcacacaccagaagatgtgtctgttctactttgttaagccgtaattatggactctcgtccttctcgttctattttccatcataaacaaccgctagggggatctcagaaaatgtagaagccttttgtttttgataaaaaagaaattaARS4Cen622,06022,08022,10022,12022,14022,16022,18022,20022,220
    ttctttttttactttctatttttaatttatatatttatattaaaaaatttaaattataattatttttatagcacgtcatctgcgagctcGATCCTTTGATCTTTTCTACGGGGTCtgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcattctaggtactaaagaaaaaaatgaaagataaaaattaaatatataaatataattttttaaatttaatattaataaaaatatcgtgcagtagacgctcgagCTAGGAAACTAGAAAAGATGCCCCAGactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtacgtaagatccatgatSwaICen622,24022,26022,28022,30022,32022,34022,36022,38022,400
    aaacaattcatccagtaaaatataatattttattttctcccaatcaggcttgatccccagtaagtcaaaaaatagctcgacatactgttcttccccgatatcctccctgatcgaccggacgcagaaggcaatgtcataccacttgtccgccctgccgcttctcccaagatcaatatttgttaagtaggtcattttatattataaaataaaagagggttagtccgaactaggggtcattcagttttttatcgagctgtatgacaagaaggggctataggagggactagctggcctgcgtcttccgttacagtatggtgaacaggcgggacggcgaagagggttctagttataminoglycoside phosphotransferase22,42022,44022,46022,48022,50022,52022,54022,560
    aagccacttactttgccatctttcacaaagatgttgctgtctcccaggtcgccgtgggaaaagacaagttcctcttcgggcttttccgtctttaaaaaatcatacagctcgcgcggatctttaaatggagtgtcttcttcccagttttcgcaatccacatcggccagatcgttatttcggtgaatgaaacggtagaaagtgtttctacaacgacagagggtccagcggcacccttttctgttcaaggagaagcccgaaaaggcagaaattttttagtatgtcgagcgcgcctagaaatttacctcacagaagaagggtcaaaagcgttaggtgtagccggtctagcaataaminoglycoside phosphotransferase22,58022,60022,62022,64022,66022,68022,70022,72022,740
    tcagtaagtaatccaattcggctaagcggctgtctaagctattcgtatagggacaatccgatatgtcgatggagtgaaagagcctgatgcactccgcatacagctcgataatcttttcagggctttgttcatcttcatactcttccgagcaaaggacgccatcggcctcactcatagtcattcattaggttaagccgattcgccgacagattcgataagcatatccctgttaggctatacagctacctcactttctcggactacgtgaggcgtatgtcgagctattagaaaagtcccgaaacaagtagaagtatgagaaggctcgtttcctgcggtagccggagtgagtaaminoglycoside phosphotransferase22,76022,78022,80022,82022,84022,86022,88022,90022,920
    gagcagattgctccagccatcatgccgttcaaagtgcaggacctttggaacaggcagctttccttccagccatagcatcatgtccttttcccgttccacatcataggtggtccctttataccggctgtccgtcatttttaaatataggttttcattttctcccaccagcttatatctcgtctaacgaggtcggtagtacggcaagtttcacgtcctggaaaccttgtccgtcgaaaggaaggtcggtatcgtagtacaggaaaagggcaaggtgtagtatccaccagggaaatatggccgacaggcagtaaaaatttatatccaaaagtaaaagagggtggtcgaatataaminoglycoside phosphotransferase22,94022,96022,98023,00023,02023,04023,06023,08023,100
    accttagcaggagacattccttccgtatcttttacgcagcggtatttttcgatcagttttttcaattccggtgatattctcattttagccatttattatttccttcctcttttctacagtatttaaagataccccaagaagctaattataacaagacgaactccaattcactgtttggaatcgtcctctgtaaggaaggcatagaaaatgcgtcgccataaaaagctagtcaaaaaagttaaggccactataagagtaaaatcggtaaataataaaggaaggagaaaagatgtcataaatttctatggggttcttcgattaatattgttctgcttgaggttaagtgacaaaminoglycoside phosphotransferase23,12023,14023,16023,18023,20023,22023,24023,260
    ccttgcattctaaaaccttaaataccagaaaacagctttttcaaagttgttttcaaagttggcgtataacatagtatcgacggagccgattttgaaaccgcggtgatcacaggcagcaacgctctgtcatcgttacaatcaacatgctaccctccgcgagatcatccgtgtttcaggaacgtaagattttggaatttatggtcttttgtcgaaaaagtttcaacaaaagtttcaaccgcatattgtatcatagctgcctcggctaaaactttggcgccactagtgtccgtcgttgcgagacagtagcaatgttagttgtacgatgggaggcgctctagtaggcacaaagtSacII23,28023,30023,32023,34023,36023,38023,40023,42023,440
    aacccggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagtctgccgccttacaacggctctcccgctgacgccgtcccggactgatgggctgcctgtatcgagtggtgattttgtgccgagctgccggtcggggagctgttggctggctggtggcaggatttgggccgtcgaatcaacggcaagaaggcttatcgtagccattgtactcgtttcagacggcggaatgttgccgagagggcgactgcggcagggcctgactacccgacggacatagctcaccactaaaacacggctcgacggccagcccctcgacaaccgaccgaccaccgtccta23,46023,48023,50023,52023,54023,56023,58023,60023,620
    atattgtggtgtaaacaaattgacgcttagacaacttaataacacattgcggacgtttttaatgtactgaattaacgccgaattaattcgggggatctggattttagtactggattttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactataacaccacatttgtttaactgcgaatctgttgaattattgtgtaacgcctgcaaaaattacatgacttaattgcggcttaattaagccccctagacctaaaatcatgacctaaaaccaaaatccttaatctttaaaataactatcttcataaaatgtttatgtttatgtatgCaMV 3'UTR (polyA signal)left ...-DNA23,64023,66023,68023,70023,72023,74023,76023,78023,800
    taagggtttcttatatgctcaacacatgagcgaaaccctataggaaccctaattcccttatctgggaactactcacacattattatggagaaactcgagcttgtcgatcgactctagctagaggatcgatccgaaccccagagtcccgctcagaagaactcgtcaagaaggcgatattcccaaagaatatacgagttgtgtactcgctttgggatatccttgggattaagggaatagacccttgatgagtgtgtaataatacctctttgagctcgaacagctagctgagatcgatctcctagctaggcttggggtctcagggcgagtcttcttgagcagttcttccgctaPspXICaMV 3'UTR (polyA signal)neomycin p...ansferase23,82023,84023,86023,88023,90023,92023,94023,960
    agaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttctcttccgctacgcgacgcttagccctcgccgctatggcatttcgtgctccttcgccagtcgggtaagcggcggttcgagaagtcgttatagtgcccatcggttgcgatacaggactatcgccaggcggtgtgggtcggccggtgtcagctacttaggtcttttcgccggtaaaagRsrIIneomycin phosphotransferase23,98024,00024,02024,04024,06024,08024,10024,12024,140
    caccatgatattcggcaagcaggcatcgccatgtgtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatggtggtactataagccgttcgtccgtagcggtacacagtgctgctctaggagcggcagcccgtacgcgcggaactcggaccgcttgtcaagccgaccgcgctcggggactacgagaagcaggtctagtaggactagctgttctggccgaaggtaggctcatgcacgagcgagctacneomycin phosphotransferase24,16024,18024,20024,22024,24024,26024,28024,30024,320
    cgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtgctacaaagcgaaccaccagcttacccgtccatcggcctagttcgcatacgtcggcggcgtaacgtagtcggtactacctatgaaagagccgtcctcgttccactctactgtcctctaggacggggccgtgaagcgggttatcgtcggtcagggaagggcgaagtcactgttgcaneomycin phosphotransferase24,34024,36024,38024,40024,42024,44024,46024,48024,500
    cgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctggagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagctcgtgtcgacgcgttccttgcgggcagcaccggtcggtgctatcggcgcgacggagcaggacctcaagtaagtcccgtggcctgtccagccagaactgtttttcttggcccgcggggacgcgactgtcggccttgtgccgccgtagtctcgtcggctaacagacaacacgggtFspIneomycin phosphotransferase24,52024,54024,56024,58024,60024,62024,64024,660
    gtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatccccatggtcgatcgacagatctgcgaaagctcgagagagatagatttgtagagagagactggtgatttcagcgtgtcctctccaaatgaaatgaacttccttatatagcagtatcggcttatcggagaggtgggttcgccggcctcttggacgcacgttaggtagaacaagttaggggtaccagctagctgtctagacgctttcgagctctctctatctaaacatctctctctgaccactaaagtcgcacaggagaggtttactttacttgaaggaatatatcNcoICaMV35S2; CaMV 35S promoter, duplicatedneomycin phosphotransferase24,68024,70024,72024,74024,76024,78024,80024,82024,840
    aggaaggtcttgcgaaggatagtgggattgtgcgtcatcccttacgtcagtggagatatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaacgatatccttccagaacgcttcctatcaccctaacacgcagtagggaatgcagtcacctctatagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctacgaggagcacccacccccaggtagaaaccctggtgacagccgtctccgtagaacttgctatPshAICaMV35S2; CaMV 35S promoter, duplicated24,86024,88024,90024,92024,94024,96024,98025,00025,020
    gcctttcctttatcgcaatgatggcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattaccctttgttgaaaagtctcaatagccctttggtcttctgagactgtatctttgatattctcggaaaggaaatagcgttactaccgtaaacatccacggtggaaggaaaagatgacaggaaaactacttcactgtctatcgacccgttaccttaggctcctccaaagggctataatgggaaacaacttttcagagttatcgggaaaccagaagactctgacatagaaactataagaCaMV35S2; CaMV 35S promoter, duplicated25,04025,06025,08025,10025,12025,14025,16025,18025,200
    tggagtagacgagagtgtcgtgctccaccatgttatcacatcaatccacttgctttgaagacgtggttggaacgtcttctttttccacgatgctcctcgtgggtgggggtccatctttgggaccactgtcggcagaggcatcttgaacgatagcctttcctttatcgcaatgatgacctcatctgctctcacagcacgaggtggtacaatagtgtagttaggtgaacgaaacttctgcaccaaccttgcagaagaaaaaggtgctacgaggagcacccacccccaggtagaaaccctggtgacagccgtctccgtagaacttgctatcggaaaggaaatagcgttactacPshAICaMV35S2; CaMV 35S promoter, duplicated25,22025,24025,26025,28025,30025,32025,34025,360
    gcatttgtaggtgccaccttccttttctactgtccttttgatgaagtgacagatagctgggcaatggaatccgaggaggtttcccgatattaccctttgttgaaaagtctcaatagccctttggtcttctgagactgtatctttgatattcttggagtagacgagagtgtcgtgccgtaaacatccacggtggaaggaaaagatgacaggaaaactacttcactgtctatcgacccgttaccttaggctcctccaaagggctataatgggaaacaacttttcagagttatcgggaaaccagaagactctgacatagaaactataagaacctcatctgctctcacagcacgCaMV35S2; CaMV 35S promoter, duplicated25,38025,40025,42025,44025,46025,48025,50025,52025,540
    tccaccatgttggcaagctgctctagccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatGTGAGTTAGCTCACTCATTAGGCAactagaaggtggtacaaccgttcgacgagatcggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattaCACTCAATCGAGTGAGTAATCCGTtgatct25,56025,58025,60025,62025,64025,66025,68025,700
    • Plasmid
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    25711 bp17500200002250025005000UBQ10 promoterUBQ10f primer binding2210-2212RareCut-MluIAscISbfI,tOCS,pUBQ1,pUBQ1_gRNA_Target1,PAM,SbfI,AscI,PspOMI,ApaI,+5SV40NLSGS12b,VENUS-N7,first diff from YFP,GS12c,pr268luc+ geneSTOP,PaqCItUBQ1,SalIU6 promoter paperpUBQ1_gRNA_Target1,gRNA Handle,U6 terminator paperAcc65I,KpnINruIpBR322 origin of replicationHpaILEU2 PromoterLEU2LEU2 TerminatorARS4Cen6,Cen6,SwaIaminoglycoside phosphotransferase,SacIIleft border repeat from C58 T-DNA,CaMV 3'UTR (polyA signal),PspXI,RsrIIneomycin phosphotransferase,CaMV35S2; CaMV 35S promoter, duplicated,FspI,NcoI,+2

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    25711