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pBT2dR3-proB T7 RNAP activation BE-PACE selection plasmid
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    aagaggacatccggtcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttgctgaggagacttAgggactcTcacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtaATAtatttctcctgtaggccagtttattttgctttccgagtcagctttctgacccggaaagcaaaacgactcctctgaaTccctgagAgtgtcgattgtggtgcagcagggatagacgacgggatccagatactcaccaacgacctattgaaatgcccgtacgtattccgagcatTATataAhdINsiISphIAvrIIBmgBIinsulated proBrrnB1 rho-independe...ptional terminatorstem loopUSER...ionT-rich20406080100120140160
    attcagggagaccacaacggtttccctctacaaataattttgtttaacttttactagagtgggaccctacctgcaggtgcagtACTACATCATCAGGCatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgataagtccctctggtgttgccaaagggagatgtttattaaaacaaattgaaaatgatctcaccctgggatggacgtccacgtcaTGATGTAGTAGTCCGtacttgtgctaattgtagcgattcttgctgaagagactgtagcttgaccgacgatagggcaagttgtgagaccgactBsaIBsgIKflIPstISbfIScaIBsaBIPaqCIPpuMIEcoO109IMN2TI4NI6AK8ND10FS12DI14EL16AA18IP20FN22TL24AD26274-2925insulated proBT7 RNA polymeraseR3 s....4k180200220240260280300320340
    ccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcggtaatgccactcgcaaatcgagcgcttgtcaaccgggaactcgtactcagaatgctctacccacttcgtgcgaaggcgttctacaaactcgcagttgaatttcgaccactccaacgcctattgcgacggcggttcggagagtagtgatgggatgagggattctactaacgtgcgNruIHY28GE30RL32AR34EQ36LA38LE40HE42SY44EM46GE48AR50FR52KM54FE56RQ58LK60AG62EV64AD66NA68AA70KP72LI74TT76LL78PK80MI82AR84274-2925T7 RNA polymerase360380400420440460480500520
    atcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtctagttgctgaccaaactccttcactttcgattcgcgccgttcgcgggctgtcggaaggtcaaggacgttctttagttcggccttcggcatcgcatgtagtggtaattctggtgagaccgaacggattggtcacgactgttatgttggcaagtccgacatcgttcgcgttagccagAlwNIIN86DW88FE90EV92KA94KR96GK98RP100TA102FQ104FL106QE108IK110PE112AV114AY116IT118IK120TT122LA124CL126TS128AD130NT132TV134QA136VA138SA140IG142274-2925T7 RNA polymerase540560580600620640660680700
    gggccattgaggacgaggctcgcttcggtcgtatccgtgAccttAaagcTaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtgcccggtaactcctgctccgagcgaagccagcataggcacTggaaTttcgAttcgtgaagttctttttgcaactccttgttgagttgttcgcgcatcccgtgcagatgttctttcgtaaatacgttcaacagctccgactgtacgagagattcccagatgagccaccgctccgcacAclIBmgBIRA144IE146DE148AR150FG152RI154RD156LK158AK160HF162KK164NV166EE168QL170NK172RV174GH176VY178KK180AF182MQ184VV186EA188DM190LS192KG194LL196GG198EA200W274-2925T7 RNA polymerase720740760780800820840860
    gtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtcagaagcaccgtattccttctgagataagtacatcctcatgcgacgtagctctacgagtaactcagttggccttaccaatcgaatgtggcggttttacgaccgcatcatccagttctgagactctgatagcttgagcgtggacttatgcgactccgatagcgttgggcacgtccaBstXIPaqCIS202SW204HK206ED208SI210HV212GV214RC216IE218ML220IE222ST224GM226VS228LH230RQ232NA234GV236VG238QD240SE242TI244EL246AP248EY250AE252AI254AT256RA258G274-2925T7 RNA polymerase8809009209409609801,0001,0201,040
    gcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacacgcgaccgaccgtagagaggctacaaggttggaacgcatcaaggaggattcggcacctgaccgtaatgaccaccaccgataacccgattgccagcagcaggagaccgcgaccacgcatgagtgtcattctttcgtgactacgcgatgcttctgcaaatgtacggactccacatgtBsgIBsrGIBsu36IA260LA262GI264SP266MF268QP270CV272VP274PK276PW278TG280IT282GG284GY286WA288NG290RR292PL294AL296VR298TH300SK302KA304LM306RY308ED310VY312MP314EV316Y274-2925T7 RNA polymerase1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcttcgctaattgtaacgcgttttgtggcgtaccttttagttgttctttcaggatcgccagcggttgcattagtggttcaccttcgtaacaggccagctcctgtagggacgctaactcgcacttcttgagggctactttggccttctgtagctgtacttaggactccgagagtggcgFspIBsu36IK318AI320NI322AQ324NT326AW328KI330NK332KV334LA336VA338NV340IT342KW344KH346CP348VE350DI352PA354IE356RE358EL360PM362KP364ED366ID368MN370PE372AL374TA376274-2925T7 RNA polymerase1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcacctttgcacgacggcgacgacacatggcgttcctgttccgagcgttcagagcggcatagtcggaactcaagtacgaactcgttcggttattcaaacgattggtattccggtagaccaagggaatgttgtacctgaccgcgccagcacaaatgcgacacagttacaagttgggcWK378RA380AA382AV384YR386KD388KA390RK392SR394RI396SL398EF400ML402EQ404AN406KF408AN410HK412AI414WF416PY418NM420DW422RG424RV426YA428VS430MF432NP434274-2925T7 RNA polymerase1,4201,4401,4601,4801,5001,5201,5401,560
    caaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcggttccattgctatactggtttcctgacgaatgcgaccgctttccatttggttagccattccttccaatgatgaccgacttttaggtgccacgtttgacacgcccacagctattccaaggcaagggactcgcgtagttcaagtaactccttttggtgctcttgtagtaccgaacgcQG436ND438MT440KG442LL444TL446AK448GK450PI452GK454EG456YY458WL460KI462HG464AN466CA468GV470DK472VP474FP476ER478IK480FI482EE484NH486EN488IM490AC492274-2925T7 RNA polymerase1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccggattcagaggtgacctcttgtgaaccacccgactcgttctaagaggcaagacgaaggaacgcaagacgaaactcatgcgaccccatgtcgtggtgccggactcgatattgacgagggaaggcgaccgcaaactgcccagaacgagaccgtaggtcgtgaagaggcgctacgaggcAK494SP496LE498NT500WW502AE504QD506SP508FC510FL512AF514CF516EY518AG520VQ522HH524GL526SY528NC530SL532PL534AF536DG538SC540SG542IQ544HF546SA548ML550R274-2925T7 RNA polymerase1,7601,7801,8001,8201,8401,8601,8801,9001,920
    agatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtctctactccatccaccagcgcgccaattgaacgaaggatcactttggcaagtcctgtagatgccctaacaacgattctttcagttgctctaagatgttcgtctgcgttagttaccctggctattgcttcatcaatggcactggctactcttgtgaccactttagagactctttcagHpaID552EV554GG556RA558VN560LL562PS564ET566VQ568DI570YG572IV574AK576KV578NE580IL582QA584DA586IN588GT590DN592EV594VT596VT598DE600NT602GE604IS606EK608V274-2925T7 RNA polymerase1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcattcgacccgtgattccgtgaccgaccagttaccgaccgaatgccacaatgagcgtcacactgattcgcaagtcagtactgcgaccgaatgcccaggtttctcaagccgaaggcagttgttcacgaccttctatggtaagtcggtcgataactaaggccgttcccagactacaagtBspHIK610LG612TK614AL616AG618QW620LA622YG624VT626RS628VT630KR632SV634MT636LA638YG640SK642EF644GF646RQ648QV650LE652DT654IQ656PA658ID660SG662KG664LM666F274-2925T7 RNA polymerase2,1202,1402,1602,1802,2002,2202,2402,260
    ctcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcagagtcggcttagtccgacgacctatgtaccgattcgactaaacccttagacactcgcactgccaccatcgacgccaacttcgttacttgaccgaattcagacgacgattcgacgaccgacgactccagtttctattcttctgacctctctaagaagcgttcgcaacgcgacacgtAflIIT668QP670NQ672AA674GY676MA678KL680IW682ES684VS686VT688VV690AA692VE694AM696NW698LK700SA702AK704LL706AA708EV710KD712KK714TG716EI718LR720KR722CA724VH726274-2925T7 RNA polymerase2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacaacccattgaggactaccaaagggacacaccgtccttatgttcttcggataagtctgcgcgaacttggactacaaggagccagtcaaggcgaatgtcggatggtaattgtggttgtttctatcgctctaactacgtgtgtttgtcctcagaccatagcgaggattgaaacatgtgBsrGIWV728TP730DG732FP734VW736QE738YK740KP742IQ744TR746LN748LM750FL752GQ754FR756LQ758PT760IN762TN764KD766SE768ID770AH772KQ774ES776GI778AP780NF782VH784274-2925T7 RNA polymerase2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggtcggttctgccatcggtggaagcattctgacatcacacccgtgtgctcttcatgccttagcttagaaaacgtgactaagtgctgaggaagccatggtaaggccgactgcgacgcttggacaagtttcgtcacgcgctttgataccaactgtgtatactcagaacactacatgaccBanIKpnINdeIAcc65ISQ786DG788SH790LR792KT794VV796WA798HE800KY802GI804ES806FA808LI810HD812SF814GT816IP818AD820AA822NL824FK826AV828RE830TM832VD834TY836ES838CD840VL842274-2925T7 RNA polymerase2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcCgacttcgcgttcgcCtGGACCAGGGCAGCAAACGACGAAAACTACAACTACGCTTTAGCAGCTTAATAgactaaagatgctggtcaagcgactggtcaacgtgctcagagttaacctgttttacggtcgtgaaggccgatttccattgaacttggaggcactgtagaatctcagGctgaagcgcaagcgGaCCTGGTCCCGTCGTTTGCTGCTTTTGATGTTGATGCGAAATCGTCGAATTATMfeIEcoNIAD844FY846DQ848FA850DQ852LH854ES856QL858DK860MP862AL864PA866KG868NL870NL872RD874IL876ES878DF880AF882AW884274-2925A1A2N3D4E5N6Y7N8Y9A10L11A12A13*14*152932-2976T1R2T7 RNA polymerasestrong ssrA degrongRNA bi...TC4C5A2,8202,8402,8602,8802,9002,9202,9402,960
    AActtaattaacggcacTcctcagcaaaggcactcctcagcaaatataatgaccctcttgataacccaagagggcattttttaatgcccatggcgtttatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagTTgaattaattgccgtgAggagtcgtttccgtgaggagtcgtttatattactgggagaactattgggttctcccgtaaaaaattacgggtaccgcaaataaacggctgatggaaccactagagcggaaagtgcatcacctgtttaagaaggttgactagacgcgcgctccggttcApoINcoIPacIBsaAIBssHII*260KG258VV256KT254IE252GK250VY248HV246FE244EL242QD240AR238SA236L3072-3851aadA (aminoglycoside adenylyl transferase)tetA/orfL bidirectional terminatorChen, Voigt e...th 0.84±0.56Chen, Voigt ...th 1.11±0.3USE...on2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccaggctagaagaagaacaggttctattcggacagatcgaagttcatactgcccgactatgacccggccgtccgcgaggtaacgggtcagccgtcgctgtaggaagccgcgctaaaacggccaatgacgcgacatggtttacgccctgttgcattcgtgatgtaaagcgagtagcggtcBglINaeIBsrFIBsrFINgoMIVR234DE232EQ230GL228YA226QR224AE222LI220VP218QY216QA214PL212RE210MA208WD206AA204VD202KP200AI198KG196TV194AS192YW190IR188SL186TL184VV182NR180ED178GA1763072-3851aadA (aminoglycoside adenylyl transferase)3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagggtcagcccgccgctcaaggtatcgcaattccaaagtaaatcgcggagtttatctaggacaagtccttggcctagtttctcaaggaggcggcgacctggatggttccgttgcgatacaagagaacgaaaacagtcgttctatcggtctagttacagctagcaccgaccgagcttPvuIBstYIWD174PP172SN170WL168TL166TE164NL162AE160FL158DQ156EP154VP152DF150LE148EA146AP144GV142LA140VS138HE136RA134KT132LL130IA128LD126ID124IT122AP120EF1183072-3851aadA (aminoglycoside adenylyl transferase)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgcctatggacgttcttacagtaacgcgacggtaagaggtttaacgtcaagcgcgaatcgacctattgcggtgccttactacagcagcacgtgttgttaccactgaagatgtcgcgcctcttagagcgagagaggtccccttcggcttcaaaggttttccagcaactagtttcgagcgAjuIBclIIG116AL114ID112NR110QW108EG106FQ104LE102RK100AP98YR96WP94II92DD90HV88VI86TV84EV82AR80LI78ES76EG74PS72AS70TE68LL66DN64IL62AR603072-3851aadA (aminoglycoside adenylyl transferase)3,5203,5403,5603,5803,6003,6203,6403,660
    cgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcagcgcaacaaagtagttcggaatgccagtggcattggtcgtttagttatagtgacacaccgaagtccggcggtaggtgacgcctcggcatgtttacatgccggtcgttgcagccaagctctaccgcgagctactgcggttgatggagactatcaactcagctatgaagccgctagtAcuIEaeIBsaHIBstEIIRT58TE56DL54RV52TV50TV48LL46DI44DS42HP40KL38GG36DV34AS32GY30LH28VA26LL24TP22EL20HR18EI16VG14VV12ES10LQ8TS6VE4AI2V3072-3851aadA (aminoglycoside adenylyl transferase)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ccgcttccctcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcgTatacatatttgaatgtatttagaaaaataggccaaatagggtttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggcgaagggagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcAtatgtataaacttacataaatctttttatccggtttatcccaaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgEarISspIBspHIBstYIBstZ17IA4E3R2M13852-3863aadA promoterColE1 origin: 15-20 copiesaad...e)-103,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggtggtttgtttgccggatcaagagctaccacctctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctccaccaaacaaacggcctagttctcgatggtggagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgaAcuIAlwNIColE1 origin: 15-20 copies4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttccgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagColE1 origin: 15-20 copies4,2204,2404,2604,2804,3004,3204,3404,360
    ccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggataccttDrdIColE1 origin: 15-20 copies4,3804,4004,4204,4404,4604,4804,5004,5204,540
    aaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaatttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttPciIBsrBIAflIIIterm4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcggatgagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgccgcctactcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgNdeIBsaAIPflFIBstZ17ITth111I4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcaactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtDrdIPfoIBsmBIEsp3IPvuIIRop4,9204,9404,9604,9805,0005,0205,0405,060
    tccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccRop-105,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttactgatgatgaacaatgactactacttg5,260
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    5265 bp37504000425045004750500025050075010001250T7 RNA polymerasestem loop274-2925USER junction,rrnB1 rho-independent transcriptional terminator,T-rich,loop,+22BsaBI,NruIAlwNIE158K mutation from cloningAclI,BmgBIBstXIPaqCIBsgIBsu36I,BsrGIFspIBstYIAcuIC>T here plus rop deletion gives pUC originAlwNIColE1 origin: 15-20 copiesDrdIterm,AflIII,PciIBsrBI,NdeIBstZ17I,BsaAI,PflFI,Tth111I,DrdI,PfoI,BsmBI,Esp3IRop,13.5k t.i.r.,-10,-35,PvuII

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    5265