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pBT29-splitC (2nd generation M13 backbone donor)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cttAgaagagcTccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgaatctgaaagaggagaaaggatctatggcgagtagcgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaagaaTcttctcgAgggatagtcactatctctaactgtagggatagtcactatctctatgactcgtgcttagactttctcctctttcctagataccgctcatcgcttctgcaatagtttctcaagtacgcaaagtttcaagcataccttccaaggcaattgccagtgctcaagctttHpaIPsrISacISapIBspQIBssSIBstBIBssSαIHincIIEco53kImRFPtet promoter20406080100120140160
    tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttagcttccacttccacttccagcaggcatgcttccatgggtctggcgatttgactttcaatggtttccaccaggcgacggcaagcgaaccctgtaggacaggggcgtcaaggtcatgccaaggtttcgaatgcaatttgtgggccgactgtagggcctgatggactttgacaggaaAhdIBaeIKpnIPfoIBsiWIAcc65IHindIIImRFP180200220240260280300320340
    cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagctgggccttccaaagtttacccttgcacaatacttgaagcttctgccaccacaacaatggcaatgggtcctgaggagggacgttctgccactcaagtagatgtttcaatttgacgcaccatggttgaagggcaggctgccaggccaatacgtctttttttggtacccaacccttcgaBaeIBtgIKpnINcoIPfoIStyIBstBIPflMIRsrIIAcc65IHindIIImRFP360380400420440460480500520
    tccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccaggtggcttgcatacatgggccttctgccacgagactttccactttagttttacgcagactttgactttctgccaccagtgatgctgcgacttcaattttggtggatgtaccgattttttggccaagtcgacggcccacgaatgttttggctgtagtttgacctgtagtggagggAcuIAgeIBstXIPshAIPvuIImRFP540560580600620640660680700
    acaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctgttgcttctgatgtggtagcaacttgtcatgcttgcacgacttccagcagtgaggtggccacgaattattcctagaggtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgcgagagatgatctcagtgtgaccgAcuIAgeIBpmIPsrISgrAIterminatormRFPrrnB1 rho-independe...ptional terminatorstem loopT-rich720740760780800820840860
    tcaccttcgggtgggcctttctgcgtttAtacaggtggcactttTcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggaagtggaagcccacccggaaagacgcaaaTatgtccaccgtgaaaAgcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctXmnIBciVIBspHIaph (KanR)terminatorUSER ...tion8809009209409609801,0001,0201,040
    ttatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcacaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtgPflMIaph (KanR)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    catgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacagtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgtAjuINsiIPvuIAsiSIBsiEIBsmBIEsp3Iaph (KanR)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    attacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagataatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctBsmIBsmINsiISmaIXmaIEcoNITspMIaph (KanR)1,4201,4401,4601,4801,5001,5201,5401,560
    ggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttaClaINruIBspDIaph (KanR)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaAggccaaataggccgTtcagatccttccgtatttagccagtatgttctctagtgtggttcgtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactTccggtttatccggcAagtctaggaaggcataaatcggtcatacaagagatcacaccaagBglISfiIBspHI*I316RG314YK312AL310IN308EL306TT304R1882-2832aph (KanR)NOT NECESSARILY SUFFIC... - context-dependent?repAUSER ...tion1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttcaacaaaaacgcactcggtactcttgcttggtaactctagtacgaatgaaacgtacagtgagtttttaaaacggagttttgaccactcgacttaaaaacgtcaatttcgtagcacatcacaaaaagaatcaggcaatgcatccatccttagactacattaccaacaaccataaaaSnaBIQ302KQ300TL298WS296FS294GN292LD290HK288SQ286MD284SL282FK280AE278FS276TL274QI272KA270TL268AD266HL264TK262RL260GN258RL256YS254DS252TI250TT248PI246K1882-2832repA1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttacagtggtaagtaaaaatagaccaacaagagttcaagccaatgctctaggtaaacagatagatcaagttgaaccttttagttgcatagtcagcccgccggagcgaatagttggtggttaaagtataacgacattcacaaatttagaaatgaataaccaaagttttgggtaaccaatBpuEID244GN242MK240IQ238NN236EL234ET232VL230DM228QR226DL224EV222QF220IL218TD216TP214RG212RK210DV208VL206KM204NS202YT200NL198DK196SI194PK192LV190WQ188N1882-2832repA2,1202,1402,1602,1802,2002,2202,2402,260
    agccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatatttcggaaaatttgagtaccatcaataaaagttcgtaattgtacttgaatttaagtagttccgattagagatataaacggaacactcaaaagaaaacacaatcaagaaaattattggtgagtatttaggagtatctcataaacaaaagttttctgaattgtacaaggtctaatataaL186RK184FE182HY180NN178EL176ML174MF172KF170ED168LS166IE164IN162AK160HT158KK156QT154LE152KL150LW148EY146IR144MS142YK140NE138FS136KV134HE132LN130YK1281882-2832repA2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaaaatacttaaaaaaattgaccttttctattccgttatagagaagtgatttttgattaagattaaaaagcgaactcttgaaccgtatcaaacaggtgaccttttagagtttcggaaattggtttcctaaggactaaaggtgtcaagagcagtagtcgagagaccaacgaaatcgattBpuEIIF126KK124LQ122FL120YP118LI116EE114SF112VL110EL108KE106SS104FK102AY100NT98WQ96FI94EF92GK90VL88PN86RI84EV82TR80TM78LE76RT74AK72AL701882-2832repA2,4602,4802,5002,5202,5402,5602,5802,6002,620
    tacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagtatgtggtattcgtaaaagggatgactacaagtagtagactcgcataaccaatattcacttgctatggcaggcaagaaaggaacatcccaaaagttagcaccccaactcatcacggtgtgtcgtattttaatcgaaccaaagtacgaggcaattcagtatcgctgattagcgatcaVG68YA66NE64RS62IN60MM58QA56YQ54NY52TF50SV48TR46EK44RT42PN40EI38TP36NL34LA32VC30CL28IL26KT24EH22ET20LD18YR16SI14AL121882-2832repA2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctaagtaaacgaaacttttgttgattaagtctgtatgtagagttaaccagatccactaaaattagtgatatggttaactctacccgatcagttactattaatgatcaggaaaaggaaactcaacacccatagacatttaagacgatctggaaacgaccttttgaacatttaagacgatMfeIMfeISpeIEN10AK8FV6VL4ES2M1882-2832pSC101 orirepA2,8202,8402,8602,8802,9002,9202,9402,960
    gaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctctgggagacatttaaggcgatctggaaacacacaaaaaaaacaaatataagttcaccaatattaaatatcttatttctttcttatttttttctatttttcttatctagggtcgggacacatattgagtgatgaaatcagtcaaggcgtcataatgttttcctacagcgtttgcgaBseYIpSC101 ori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaacaaacgaggagatgttttgtctggaattttgggatttccgaattcatcgtgggagcgttcgagcccgtttagcgacttataaggaaaacagaggctggtagtccgtggactcagcgacagaaaaagcactgtaagtcaagcgacgcgagtgccgagaccgtcacttacccccattBcgIAflIIpSC101 oripartitioning locus, gyrase binding3,1603,1803,2003,2203,2403,2603,2803,3003,320
    atggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggatttaccgtgatgtccgcggaaaatacctaagtacgttcctttgatgggtattatgttcttttcgggcagtgcccgaagagtcccgcaaaataccgcccagacgatacaccacgatagactgaaaaacgacaagtcgtcaaggacgggagactaaaaggtcagactggtgaagcctaaKasINarISfcISfoIAlwNIBsaHIPluTIBstAPIpSC101 oripartitioning locus, gyrase bindinghairpin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    atcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtcaagaggacatccggtcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttgctgaggagacttagggaccctgctcttctGGCttagggcactgtccagtaagtctgaccgattacgtgggtcattccgtcgccatagtagttgtccgaatgggcagaatgacagttctcctgtaggccagtttattttgctttccgagtcagctttctgacccggaaagcaaaacgactcctctgaatccctgggacgagaagaCCGaAhdIKflISapIBbvCIBspQIPpuMIpSC101 orirrnB1 rho-independe...ptional terminatorstem loopUSER...ionT-rich3,5203,5403,5603,5803,6003,6203,6403,660
    ctagaaggagattttcaacatgctccctcaatcggttgaatgtcgcccttttgtctttggcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtatCttctacgtttgctgatcttcctctaaaagttgtacgagggagttagccaacttacagcgggaaaacagaaaccgcgaccatttggtatacttaaaagataactaacactgttttatttgaataaggcaccacagaaacgcaaagaaaatatacaacggtggaaatacatacataGaagatgcaaacgaBtgINdeIXbaIfragment of gIIIgVI promoter3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aacatactgcgtaataaggagtcttaatcatgccagttcttttgggtattccgttattattgcgtttcctcggtttccttctggtaactttgttcggctatctgcttacttttctCaaaaagggcttcggtaagatagctattgctatttcattgtttcttgctcttattattggttgtatgacgcattattcctcagaattagtacggtcaagaaaacccataaggcaataataacgcaaaggagccaaaggaagaccattgaaacaagccgatagacgaatgaaaagaGtttttcccgaagccattctatcgataacgataaagtaacaaagaacgagaataataaccCspCIgVIfragment of gIII3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gcttaactcaattcttgtgggttatctctctgatattagTgctcaattaccctctgactttgttcagggtgttcagttaattctcccgtctaatgcgcttccctgtttttatgttattctctctgtaaagActgctattttcatttttgacgttaaacaaaaaatcgtttcttatcgaattgagttaagaacacccaatagagagactataatcAcgagttaatgggagactgaaacaagtcccacaagtcaattaagagggcagattacgcgaagggacaaaaatacaataagagagacatttcTgacgataaaagtaaaaactgcaatttgttttttagcaaagaatagVIgI promoter4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ttggattgggaCaaataatatggctgtttattttgtaactggcaaattaggctctggaaagacgctcgttagcgttggtaagattcaggataaaattgtagctgggtgcaaaatagcaactaatcttgatttaaggcttcaaaacctcccgcaagtcgggaggttcgctaaaacgaacctaaccctGtttattataccgacaaataaaacattgaccgtttaatccgagacctttctgcgagcaatcgcaaccattctaagtcctattttaacatcgacccacgttttatcgttgattagaactaaattccgaagttttggagggcgttcagccctccaagcgattttgcBseYIgIgVI4,2204,2404,2604,2804,3004,3204,3404,360
    cctcgcgttcttagaataccggataagccttctatatctgatttgcttgctattgggcgcggtaatgattcctacgatgaaaataaaaacggAttgcttgttctcgatgagtgcggtacttggtttaatacccgttcttggaatgataaggaaagacagccgattattgattggtggagcgcaagaatcttatggcctattcggaagatatagactaaacgaacgataacccgcgccattactaaggatgctacttttatttttgccTaacgaacaagagctactcacgccatgaaccaaattatgggcaagaaccttactattcctttctgtcggctaataactaaccaAloIgI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ttctacatgctcgtaaattaggatgggatattatttttcttgttcaggacttatctattgttgataaacaggcgcgttctgcattagctgaacatgttgtttattgtcgtcgtctggacagaattactttaccttttgtcggtactttatattctcttattactggctcgaaaataagatgtacgagcatttaatcctaccctataataaaaagaacaagtcctgaatagataacaactatttgtccgcgcaagacgtaatcgacttgtacaacaaataacagcagcagacctgtcttaatgaaatggaaaacagccatgaaatataagagaataatgaccgagcttttagI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcctctgcctaaattacatgttggcgttgttaaatatggcgattctcaattaagccctactgttgagcgttggctttatactggtaagaatttgtataacgcataCgatactaaacaggctttttctagtaattatgattccggtgtttattcttatttaacgccttatttatcacggagacggatttaatgtacaaccgcaacaatttataccgctaagagttaattcgggatgacaactcgcaaccgaaatatgaccattcttaaacatattgcgtatGctatgatttgtccgaaaaagatcattaatactaaggccacaaataagaataaattgcggaataaatagtgI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cacggtcggtatttcaaaccattaaatttaggtcagaagatgaaattaactaaaatatatttgaaaaagttttctcgcgttctttgtcttgcgattggatttgcatcagcatttacatatagttatataacccaacctaagccggaggttaaaaaggtagtctctcagacctatggtgccagccataaagtttggtaatttaaatccagtcttctactttaattgattttatataaactttttcaaaagagcgcaagaaacagaacgctaacctaaacgtagtcgtaaatgtatatcaatatattgggttggattcggcctccaatttttccatcagagagtctggatacBsiEIgIgXI4,9204,9404,9604,9805,0005,0205,0405,060
    attttgataaattcactattgactcttctcaTcgtcttaatctaagctatcgctatgttttcaaggattctaagggaaaattaattaatagcgacgatttacagaagcaaggttattcactTacatatattgatttatgtactgtttccattaaaaaaggtaattcaaatgaaattaaaactatttaagtgataactgagaagagtAgcagaattagattcgatagcgatacaaaagttcctaagattcccttttaattaattatcgctgctaaatgtcttcgttccaataagtgaAtgtatataactaaatacatgacaaaggtaattttttccattaagtttactttaPacITatIgIgXIgIV promoter5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tgttaaatgtaattaattttgttttcttgatgtttgtttcatcatcttcttttgctcaggtaattgaaatgaataattcgcctctgcgcgattttAtaacttggtattcaaagcaatcaggcgaatccgttattgtttctcccgatgtaaaaggtactgttactgtatattcatcacaatttacattaattaaaacaaaagaactacaaacaaagtagtagaagaaaacgagtccattaactttacttattaagcggagacgcgctaaaaTattgaaccataagtttcgttagtccgcttaggcaataacaaagagggctacattttccatgacaatgacatataagtaggIVgIgXI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tgacgttaaacctgaaaatctacgcaatttctttatttctgttttacgtgcaaataattttgatatggtaggttctaacccttccattattcagaagtataatccaaacaatcaggattatattgatgaattgccatcaCctgataatcaggaatatgatgataattccgctcctactgcaatttggacttttagatgcgttaaagaaataaagacaaaatgcacgtttattaaaactataccatccaagattgggaaggtaataagtcttcatattaggtttgttagtcctaatataactacttaacggtagtGgactattagtccttatactactattaaggcgaggagIV5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tctggtggtttctttgtCccgcaaaatgataatgttactcaaacttttaaaattaataacgttcgggcaaaggatttaatacgagttgtcgaattgtttgtaaagtctaatacttctaaatcctcaaatgtattatctattgacggAtctaatctattagttgttagtgctcctaagaccaccaaagaaacaGggcgttttactattacaatgagtttgaaaattttaattattgcaagcccgtttcctaaattatgctcaacagcttaacaaacatttcagattatgaagatttaggagtttacataatagataactgccTagattagataatcaacaatcacgaggatAclIgIV5,6205,6405,6605,6805,7005,7205,7405,760
    aagatattttagataaccttTctcaattcctttcaactgttgatttgccaactgaccagGtattgattgagggtttgatatttgaggttcagcaaggtgatgctttagatttttcatttgctgctggctctcagcgtggcactgttgcaggcggAgttaatactgaccgcctcacttctataaaatctattggaaAgagttaaggaaagttgacaactaaacggttgactggtcCataactaactcccaaactataaactccaagtcgttccactacgaaatctaaaaagtaaacgacgaccgagagtcgcaccgtgacaacgtccgccTcaattatgactggcggagtgEciISexAIgIV5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ctctgttttatcttctgctggtggttcgttcggtatttttaatggcgatgttttagggctatcagttcgcgcattaaagactaatagccattcaaaaatattgtctgtgccacgtattcttacgctttcaggtcagaagggttctatctctAttggccagaatgtcccttttattgagacaaaatagaagacgaccaccaagcaagccataaaaattaccgctacaaaatcccgatagtcaagcgcgtaatttctgattatcggtaagtttttataacagacacggtgcataagaatgcgaaagtccagtcttcccaagatagagaTaaccggtcttacagggaaaataaEaeIMscIBtgZIgIV5,9605,9806,0006,0206,0406,0606,0806,1006,120
    actggtcgtgtAactggtgaatctgccaatgtaaataatccatttcagacgattgagcgtcaaaatgtaggtatttccatgagcgtttttcctgttgcaatggctggcggtaatattgttctggatattaccagcaaggccgatagtttgagttcttctactcaggcaagtgatgtgaccagcacaTtgaccacttagacggttacatttattaggtaaagtctgctaactcgcagttttacatccataaaggtactcgcaaaaaggacaacgttaccgaccgccattataacaagacctataatggtcgttccggctatcaaactcaagaagatgagtccgttcactacBsrDIgIV6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ttattactaaCcaaagaagtattgctacaacggttaatttgcgtgatggacagactcttCtactcggtggcctcactgattataaaaacacttctcaggattctggcgtaccgttcctgtctaaaatccctttaatcggcctcctgtttagctcccgctctgattctaacgaggaaataatgattGgtttcttcataacgatgttgccaattaaacgcactacctgtctgagaaGatgagccaccggagtgactaatatttttgtgaagagtcctaagaccgcatggcaaggacagattttagggaaattagccggaggacaaatcgagggcgagactaagattgctcctBarIgIV6,3206,3406,3606,3806,4006,4206,4406,460
    GagcacgttatacgtgctcgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgCtcgtgcaatatgcacgagcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcNaeISfcINgoMIVpackaging signalgIV6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tcaagcGctaaatcgggggcccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaaagttcgCgatttagcccccggggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttAfeIAloIApaIDrdIBaeGIBtgZIDraIIIPspOMIessential (+) origincomplete (+) originRNA pri...cation6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ctggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttaaatatttgcttatacaatcttcctgtttttggggaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaatttataaacgaatatgttagaaggacaaaaacccAclISwaIcomplete (+) origingII promoteres...n6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gctttccgaaag7,005
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    7006 bpSapI Golden Gate dropout cassette: ori, KmR, RFP500055006000650050010001500gIVgIaph (KanR)gXIUSER junctionstem looploopmRFPCTT for SapI Golden Gate cloning,SapI,tet promoter,BspQI,SapI,Eco53kI,SacI,+14BstBI,Acc65I,KpnI,RsrII,BtgI,NcoI,StyI,PflMI,HindIII,BaeIPshAI,BstXI,AcuI,AgeI,PvuII,AgeI,SgrAI,AcuI,BpmIterminator,rrnB1 rho-independent transcriptional terminator,T-rich,+1BspHI,BciVI,XmnIPflMINsiIAjuI,BsmBI,Esp3I,AsiSI,BsiEI,PvuI,BsmIEcoNIBsmI,TspMI,XmaI,SmaI,NsiIBspDIClaI,NruImutated vs generation 1 backbonemutated vs generation 1 backbone,+1mutated vs generation 1 backbone,AclImutated vs generation 1 backbone,+2mutated vs generation 1 backbone,EciImutated vs generation 1 backbone,BtgZImutated vs generation 1 backbone,EaeI,MscI,BsrDImutated vs generation 1 backbone,mutated vs generation 1 backbone,BarIpackaging signal,SfcI,NgoMIV,NaeImutated vs generation 1 backbone,RNA primer for (-) replication,+14

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    7006