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XCW48 - pCOLADuet-WspR D70E
  • Sequence Map
    Editing disabled on read-only sequence.
    ggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgggcagcagccatcaccatcatcaccacagccaggatccgaattcgagctcggcgcgcctgcaggtcgacaagcttgcggccgcataatgcttaagtcgaacaccccttaacactcgcctattgttaaggggacatctttattaaaacaaattgaaattattcctctatatggtacccgtcgtcggtagtggtagtagtggtgtcggtcctaggcttaagctcgagccgcgcggacgtccagctgttcgaacgccggcgtattacgaattcagcttgtAscIBcgIEagINcoINotISacISbfIAflIIBamHIBfuAIBspMIEcoRIBssHIIBsiHKAIEco53kIHindIIINmeAIIIlacOHis-Tag20406080100120140160
    gaaagtaatcgtattgtacacggccgcataatcgaaattaatacgactcactataggggaattgtgagcggataacaattccccatcttagtatattagttaagtataagaaggagatatacatatgcacaaccctcatgagagcaagaccgacctgggcgcccctctggacggcctttcattagcataacatgtgccggcgtattagctttaattatgctgagtgatatccccttaacactcgcctattgttaaggggtagaatcatataatcaattcatattcttcctctatatgtatacgtgttgggagtactctcgttctggctggacccgcggggagacctgccgBarIEagINdeITatIBsrGIMCS-2 ORFWspRlacOZFH066T7 promoter180200220240260280300320340
    gcggtcatggtactgcttgtcgatgatcaggccatgatcggagaggctgtgcgtcgctcactggcgagcgaggcgggcatcgacttccatttctgttccgatccgcagcaggcggtggcggtggccaaccagatcaagccgacggtgatcctccaggaActggtgatgcccggcgcgccagtaccatgacgaacagctactagtccggtactagcctctccgacacgcagcgagtgaccgctcgctccgcccgtagctgaaggtaaagacaaggctaggcgtcgtccgccaccgccaccggttggtctagttcggctgccactaggaggtcctTgaccactacgggccgcBclIMscIPfoIMCS-2 ORFWspR360380400420440460480500520
    tcgacggcctcacgctgctcgctgcctaccgcggcaacccggcgacccgcgacatcccgatcatcgtcctgtcgaccaaggaagagccgacggtgaagagcgcggcgttcgccgccggcgccaacgactacctggtcaagctgccggacgccatcgagctggtggcgcggatccgagctgccggagtgcgacgagcgacggatggcgccgttgggccgctgggcgctgtagggctagtagcaggacagctggttccttctcggctgccacttctcgcgccgcaagcggcggccgcggttgctgatggaccagttcgacggcctgcggtagctcgaccaccgcgcctaggcMreISapISapIBamHIBspQIBspQISacIISgrAISgrDIMCS-2 ORFWspR540560580600620640660680700
    ctaccactcgcgttcgtacatcgccctgcagcaacgcgacgaggcctatcgggcgctgcgcgagagccagcagcagttgctggagaccaacctggtgctgcagcggctgatgaactccgacggcctgaccgggctctccaaccgtcgtcacttcgacgaatacctggagatggaggatggtgagcgcaagcatgtagcgggacgtcgttgcgctgctccggatagcccgcgacgcgctctcggtcgtcgtcaacgacctctggttggaccacgacgtcgccgactacttgaggctgccggactggcccgagaggttggcagcagtgaagctgcttatggacctctacctcBglIBsaIStuIBstAPIMCS-2 ORFWspR720740760780800820840860
    tggcgccgctcgctgcgcgagcagtcgcaactgtcgctgctgatgatcgacgtggactatttcaagagctacaacgacaccttcggccacgtcgccggcgacgaggccctgcgccaggtcgccggggccatccgcgagggctgcagtcgctcctcggacctggcggctcggtacgaccgcggcgagcgacgcgctcgtcagcgttgacagcgacgactactagctgcacctgataaagttctcgatgttgctgtggaagccggtgcagcggccgctgctccgggacgcggtccagcggccccggtaggcgctcccgacgtcagcgaggagcctggaccgccgagccatgcMreIBseRISgrAIEcoO109IMCS-2 ORFWspR8809009209409609801,0001,0201,040
    gcggcgaggagttcgccatggtcctcccgggcacctcgccgggcggcgcacggttgctggcggagaaggtccggcgcacggtggagagcctgcagatcagccacgaccagccacgcccgggctcgcacctgaccgtcagcatcggcgtcagcaccctggtgccgggcggcggcggcgccgctcctcaagcggtaccaggagggcccgtggagcggcccgccgcgtgccaacgaccgcctcttccaggccgcgtgccacctctcggacgtctagtcggtgctggtcggtgcgggcccgagcgtggactggcagtcgtagccgcagtcgtgggaccacggcccgccgccgccAleINcoISrfIBseRIMCS-2 ORFWspR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gcaaaccttccgggttctcatcgagatggccgaccaggcgctctaccaggccaagaacaatggccgcaaccaggtgggcctgatggaacagccggtgccgccggccccggcgggctgactcgagtctggtaaagaaaccgctgctgcgaaatttgaacgccagcacatggactcgcgtttggaaggcccaagagtagctctaccggctggtccgcgagatggtccggttcttgttaccggcgttggtccacccggactaccttgtcggccacggcggccggggccgcccgactgagctcagaccatttctttggcgacgacgctttaaacttgcggtcgtgtacctgagcBglITliIXhoIPshAIPspXIPaeR7IMCS-2 ORFWspRS-Tag1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tctactagcgcagcttaattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaacctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagaagatgatcgcgtcgaattaattggatccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttggagtccgtaaactcttcgtgtgccagtgtgacgaaggccatcagttatttggccatttggtcgttatctAgeIBlpIPacIAvrIIBsu36IEcoO109IT7 TerminatorT7 Ter...H067)MCS-2 ORF1,4201,4401,4601,4801,5001,5201,5401,560
    cataagcggctatttaacgaccctgccctgaaccgacgacaagctgacgaccgggtctccgcaagtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcgtattcgccgataaattgctgggacgggacttggctgctgttcgactgctggcccagaggcgttcaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtacttaattaagaatctttttgagtagctcgtagBsaIXmnIPflFITth111IKan1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgttttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttgtagttatgttggataattaaaggggagcaKan1,7601,7801,8001,8201,8401,8601,8801,9001,920
    caaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgaggtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcaataagtaagcactaacgcggactcgctcAjuINsiIBsmBIEsp3IBpu10IKan1,9401,9601,9802,0002,0202,0402,0602,0802,100
    acgaaatacgcggtcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggatgctttatgcgccagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcaccactcattggtacgtagtagtcctBsmIBsmINsiISspIKan2,1202,1402,1602,1802,2002,2202,2402,260
    gtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacatcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaacagcgtggactaacgggctgtaClaIBspDIKan2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatcttNruISspIKan2,4602,4802,5002,5202,5402,5602,5802,6002,620
    aaataaacaaataggcatgctagcgcagaaacgtcctagaagatgccaggaggatacttagcagagagacaataaggccggagcgaagccgtttttccataggctccgcccccctgacgaacatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataatttatttgtttatccgtacgatcgcgtctttgcaggatcttctacggtcctcctatgaatcgtctctctgttattccggcctcgcttcggcaaaaaggtatccgaggcggggggactgcttgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatattBmtINheINspISphIColA ori2,6402,6602,6802,7002,7202,7402,7602,7802,800
    agataccaggcgtttccccctgatggctccctcttgcgctctcctgttcccgtcctgcggcgtccgtgttgtggtggaggctttacccaaatcaccacgtcccgttccgtgtagacagttcgctccaagctgggctgtgtgcaagaaccccccgttcagcccgactgctgcgccttctatggtccgcaaagggggactaccgagggagaacgcgagaggacaagggcaggacgccgcaggcacaacaccacctccgaaatgggtttagtggtgcagggcaaggcacatctgtcaagcgaggttcgacccgacacacgttcttggggggcaagtcgggctgacgacgcggaBsmFIColA ori2,8202,8402,8602,8802,9002,9202,9402,960
    tatccggtaactatcatcttgagtccaacccggaaagacacgacaaaacgccactggcagcagccattggtaactgagaattagtggatttagatatcgagagtcttgaagtggtggcctaacagaggctacactgaaaggacagtatttggtatctgcgctccactaaagccagataggccattgatagtagaactcaggttgggcctttctgtgctgttttgcggtgaccgtcgtcggtaaccattgactcttaatcacctaaatctatagctctcagaacttcaccaccggattgtctccgatgtgactttcctgtcataaaccatagacgcgaggtgatttcggtcEcoRVColA ori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ttaccaggttaagcagttccccaactgacttaaccttcgatcaaaccgcctccccaggcggttttttcgtttacagagcaggagattacgacgatcgtaaaaggatctcaagaagatcctttacggattcccgacaccatcactctagatttcagtgcaatttatctcttcaaataatggtccaattcgtcaaggggttgactgaattggaagctagtttggcggaggggtccgccaaaaaagcaaatgtctcgtcctctaatgctgctagcattttcctagagttcttctaggaaatgcctaagggctgtggtagtgagatctaaagtcacgttaaatagagaagtttaBarIPvuIXbaIColA ori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtagcacctgaagtcagccccatacgatataagttgtaattctcatgttagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttcatcgtggacttcagtcggggtatgctatattcaacattaagagtacaatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaAcuIlacI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaBsmBIEsp3IPvuIIPvuIIlacI3,5203,5403,5603,5803,6003,6203,6403,660
    ttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctacaggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtBbsIHpaIBsrDIBssHIIlacI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgacacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctApaIBcgIPspOMINmeAIIIlacI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaataaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattaBbsIBsgIBsrDIBstEIIlacI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgctagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcBclIBsgIMluIApaLIAflIIIBsiHKAIlacI4,2204,2404,2604,2804,3004,3204,3404,360
    ccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcaBstAPIlacI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaaattaatacgactcactatatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatcctttaattatgctgagtgatatAclIPfoIlacIT7 Promoter4,5604,5804,6004,6204,6404,6604,6804,700
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4709 bp350037504000425045002505007501000MCS-2 ORFlacIWspRlacO,RBS-1,His-Tag,ZFH066,T7 promoter,lacO,NcoI,NmeAIII,BamHI,EcoRI,Eco53kI,+15RBS,BarI,NdeI,BclIMscI,PfoI,SgrDI,SacIIBspQI,SapI,BspQI,SapI,MreI,SgrAIBamHI,StuI,BglIBstAPIBsaIMreI,SgrAI,EcoO109I,BseRIif C here, this is G249A,NcoI,BseRISrfIBsmBI,Esp3IPvuIIHpaI,BbsIBssHII,BsrDIBcgIPspOMI,ApaI,NmeAIII,BstEIIBbsIBsgI,BsrDI,BclI,AflIII,MluI,ApaLI,BsiHKAIBsgIT7 Promoter,BstAPI,AclI,PfoI

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    4709