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pDCAFC
  • Sequence Map
    Editing disabled on read-only sequence.
    tttacggctagctcagtcctaggtacaatgctagctactagagATTAAAGAGGAGAAATACTAGatgtctactgaccaagtaatttttaacgactggcatccagtcgctgctttggaagatgtatctctcgataaacgataccgctgtcgactactaggtcgtacagttagttataaatgccgatcgagtcaggatccatgttacgatcgatgatctcTAATTTCTCCTCTTTATGATCtacagatgactggttcattaaaaattgctgaccgtaggtcagcgacgaaaccttctacatagagagctatttgctatggcgacagctgatgatccagcatgtcaatcaataBaeIBmtIBmtINheINheISalIStyIAvrIIMS2TD4QV6IF8ND10WH12PV14AA16LE18DV20SL22DK24RY26RC28RL30LG32RT34VS36Y65-919ndmC CDSndmC geneJ23110BBa_B0030iGEM15C-0920406080100120140160
    gtgaaaacatctgatgctgttaatgctcattgggaagaaagtgcagatgaaattaagaccatccgcgctaaagaaatctatggtcttctgtggctctcctttgccgacaaacccagtgagatgttcgatattgcagagttcaaggaacctgatcgccgaatcgtcagcgctggatcacttttgtagactacgacaattacgagtaacccttctttcacgtctactttaattctggtaggcgcgatttctttagataccagaagacaccgagaggaaacggctgtttgggtcactctacaagctataacgtctcaagttccttggactagcggcttagcagtcgcgacctaBbsIV38KT40SD42AV44NA46HW48EE50SA52DE54IK56TI58RA60KE62IY64GL66LW68LS70FA72DK74PS76EM78FD80IA82EF84KE86PD88RR90IV92SA94G65-919ndmC CDSndmC gene180200220240260280300320340
    ctgtgcgcgtaaatgtctcaggactgcgtgctatcgaaaactttctggacatggctcatttcccttttgttcatacggatattttgggtgcagagccactgacggaagttgagccgtataacgtcaactatgatgaaactgttgatgagatcttcgctactgagtgtaagttcccgacacgcgcatttacagagtcctgacgcacgatagcttttgaaagacctgtaccgagtaaagggaaaacaagtatgcctataaaacccacgtctcggtgactgccttcaactcggcatattgcagttgatactactttgacaactactctagaagcgatgactcacattcaagggXmnIS96VR98VN100VS102GL104RA106IE108NF110LD112MA114HF116PF118VH120TD122IL124GA126EP128LT130EV132EP134YN136VN138YD140ET142VD144EI146FA148TE150CK152FP15465-919ndmC CDSndmC gene360380400420440460480500520
    gcaacctaaaggctcagctactgctgtcgagccaattgatatgcagtatatataccgcattacacggccatattccgccattttatataaaacttgcccgcccgaaccgcatagatgggatgccctcggtctgtttattcaacctgtagacgaagattggtgcatagcgcatacacgttggatttccgagtcgatgacgacagctcggttaactatacgtcatatatatggcgtaatgtgccggtataaggcggtaaaatatattttgaacgggcgggcttggcgtatctaccctacgggagccagacaaataagttggacatctgcttctaaccacgtatcgcgtatgtBlpIMfeIQP156KG158SA160TA162VE164PI166DM168QY170IY172RI174TR176PY178SA180IL182YK184TC186PP188EP190HR192WD194AL196GL198FI200QP202VD204ED206WC208IA210HT21265-919ndmC CDSndmC gene540560580600620640660680700
    attatgtgctatgtggatgacgttaactcagatcaacaacttcgccatttccagcagacgatttttggccaggacttgatgattcttatcaaccaagttccaaagcgtcttcccctggctgcaagtcgagagagcccagtccgggccgatgtgcttgcaacagcttatcgtcgcttaatacacgatacacctactgcaattgagtctagttgttgaagcggtaaaggtcgtctgctaaaaaccggtcctgaactactaagaatagttggttcaaggtttcgcagaaggggaccgacgttcagctctctcgggtcaggcccggctacacgaacgttgtcgaatagcagcgaBbsIBcgIHpaIBsaBIIM214CY216VD218DV220NS222DQ224QL226RH228FQ230QT232IF234GQ236DL238MI240LI242NQ244VP246KR248LP250LA252AS254RE256SP258VR260AD262VL264AT266AY268RR27065-919ndmC CDSndmC gene720740760780800820840860
    ggctgcgtgagaaaggtgtgcaatatggtgctctgcgggactaatactagagtcacacaggaaagTACTAGatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatccgacgcactctttccacacgttataccacgagacgccctgattatgatctcagtgtgtcctttcATGATCtacttgtttgaactgcagttggtcaccaaaggataacgatggtgacttctagagggcttcgcggtacagaaagtacggtgcaacaaccccgttctttaccggtaBstXITsp45IMN2KL4DV6NQ8WF10PI12AT14TE16DL18PK20RH22VF24HA26TL28LG30QE32MA34I947-2713WL272RE274KG276VQ278YG280AL282RD284*65-919ndmD CDSndmD genendmC CDSndmC geneEQ_NdmC_revBBa...328809009209409609801,0001,0201,040
    ctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccagaccgcgctactgagaccaagtcaattacgaaccctcttggcgacgggcgtatctcctaacgccaactgtgacccacgattatggccattgctcaacgccacagtcatagtacctacctgaatactttcgccctgaccaccgacgtgaaaacagggtcgggtagcgctacgtggtAgeINruIBseYIDraIIIW36RD38DS40GS42VN44AW46EN48RC50PH52RG54LR56LT58LG60AN62TG64NE66LR68CQ70YH72GW74TY76ES78GT80GG82CT84FV86PA88HR90DA92P947-2713ndmD CDSndmD gene1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggaggggggtttacggcgcgcccaattatgaaaaggacaggcgcttttcgtgccgaaatagacctgctgtaatccagtcggcggtcctctcgggtaaagttaggagctactgcgagtcgaacatttgcgacatttttgtttagacgtatcgcatcaatatctacgactataactgcctcAhdIBlpIP94PN96AA98RV100NT102FP104VR106EK108HG110FI112WT114TL116GQ118PP120GE122PI124SI126LD128DA130QL132VN134AV136KT138NL140HS142VV144ID146AD148ID150G947-2713ndmD CDSndmD gene1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtaacagtcgcaggatgcagtcttagaaagtcgcaagtagctacacaaaccacgccggtcgcgacttctagacgtaaactttaggtacgacgttctagctccctaagaccattgttctagtccgagataacgataagtaaaaatatacgtcgcgagttggtaatcatttacgcaacaClaIBspDIBsrGIV152VS154VL156RQ158NL160SA162FI164DV166FG168AA170SA172ED174LH176LK178SM180LQ182DR184GI186LV188TR190SG192SI194AI196HF198YM200QR202ST204IS206KC208VV210947-2713ndmD CDSndmD geneiGEM15-24C1,4201,4401,4601,4801,5001,5201,5401,560
    acatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgacttgtacgcgttcatgaatgaggccctgcaggtcctatgcttgaagttttcttgatgagcatacggtacttgcaataggcgtcccgtcgtcttcgacatcgatggctgaactaatcgtaatgtctatagtcgctagtctgatagcttttcgaacttcagcaatctcttttgtactgaAclIBsaXIEcoRVHA212QV214LT216PG218RP220GY222EL224QK226NY228SY230AM232NV234IR236RA238AE240AV242AT244DL246IS248IT250DI252SD254QT256IE258KL260EV262VR264EN266MT268947-2713ndmD CDSndmD gene1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgttccgaggaggttgggtgatatagacgcttcaccaatgcgcatgagttctctgtccactataattgagtatgaccgacttcggatagccgatgggtgatggtcgtaagtcaggtccctacgtgtagtcgtagtgttgcggcctcccatcgtaagctgttataagggagcacttgcMluIBsaBIAflIIIKA270PP272TH274YI276CE278VV280TR282TQ284ET286GD288IN290SY292WL294KP296IG298YP300LP302AF304SP306GM308HI310SI312TT314PE316GS318IR320QY322SL324VN326947-2713ndmD CDSndmD gene1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggccggactgctcgcacttaggaagtagtagccacacttctttctctaagtcagggcaccgccgaggtctagttacgtgcttctacacttccaaccttgcgttgattttcaatgtgaaggctccttgccaaaaggtgagcaggtttggtcttttgtgggctaagagcatcgtccaccBfuAIBspMIPaqCIGP328DE330RE332SF334II336GV338KK340EI342QS344RG346GS348RS350MH352ED354VK356VG358TQ360LK362VT364LP366RN368GF370PL372VQ374TR376KH378PI380LV382AG384G947-2713ndmD CDSndmD gene1,9401,9601,9802,0002,0202,0402,0602,0802,100
    catcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcagtagccatagtggggttaaaacacataccgtgtccgagacctagtcgttccaagtagcatactttatgtaataaaacgagcacgtaaactcgtacaaggtaaggtcctagctgactgacgcaacccgctagcaaacttacacgtagaaccggagccgggtctgctctgatctcgtPvuII386GI388TP390IL392CM394AQ396AL398DQ400QG402SS404YE406IH408YF410AR412AF414EH416VP418FQ420DR422LT424AL426GD428RL430NV432HL434GL436GP438DE440TR442A947-2713ndmD CDSndmD gene2,1202,1402,1602,1802,2002,2202,2402,260
    aaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagtttgaacggctgtagtacctctaagtattgcgggttctgcatctacaaatgtgaacgccgggcgttggttactagctttgacatagacgacagcgagaacgagtaccgtagcgacttctcaggtaggctaaacttaaaaagtcatttttcttgctacaagggcaaagactacttcEarIBstAPIK444LA446DI448ME450IH452NA454QD456VD458VY460TC462GP464QP466MI468ET470VS472AV474AL476AH478GI480AE482ES484IR486FE488FF490SK492KN494DV496PV498SD500E947-2713ndmD CDSndmD geneiGEM15-25C2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttattatactccaactcgagtttttttgaccagtttataagtgacagagcggaccgagatgcaacaacgttcgaacaaacctgttgctacaagcatagcttcgaagaacactcgtcccacatacgccctgaacatattgaggtcagcataggccgctagagctcgtagtactgtgaatBarISacIEco53kIE502YE504VE506LK508KT510GQ512IF514TV516SP518GS520TL522LQ524AC526LD528ND530VR532IE534AS536CE538QG540VC542GT544CI546TP548VV550SG552DL554EH556HD558TY560947-2713ndmD CDSndmD gene2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaacggaaagattctttctttccctttcgccattcacctagtacggcacacaaagcgcgacgttcaggtttttttagcaagagctagacactccggcccgtagtgggtgccatacctgctcgacatatttgatctctttctcctctttatgatctactagtgcgaaatactgatacttgBclIPflMILS562KK564ER566ES568GK570WI572MP574CV576SR578CK580SK582KI584VL586DL588*947-2713MI2TL4YD6YE82776-3450ndmD CDSndmD geneEQ_gst9_1 (gBLOCK)EQ_NdmD_pSB1C3_revEQ_pSB1C3_Gib_forgst9 CDSgst9 geneBBa...BS2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcagagccccttaacgatgttccacgcctacgaggaaaggtaggacttcgatggcatgtgctatctctgtcaccttaaaaagggcaggtcccttgtatttagtggccggaaggacgcgtagttaggtgagccggtcaacggccataacgcgctactactaaagcacaacgcgctacgBarILS10GN12CY14KV16RM18LL20SI22LK24LP26YT28IE30TV32EF34FP36SR38EH40KS42PA44FL46RI48NP50LG52QL54PV56LR58DD60DF62VL64RD66A2776-3450EQ_gst9_1 (gBLOCK)gst9 CDSgst9 gene2,8202,8402,8602,8802,9002,9202,9402,960
    ccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcaggtccggtaggaccaaatggaccgcttgtttatgctgtggtcaaactggaccataggctgtgtacgcgggcggtaggagccggtccgtttatacaccgagcgcaaacggctaccaaactggccgagctaaaggcgacgtgcaaacgtactaaacaaaaaaatgctaaagttacgtBcgIEcoNISexAIQ68AI70LV72YL74AN76KY78DT80SL82TW84YP86TH88AP90AI92LG94QA96NM98WL100AF102AD104GL106TG108SI110SA112AR114LH116DL118FF120YD122FN124A2776-3450gst9 CDSgst9 geneEQ_gst9_1 (gBLOCK)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactctcgttacggcacgggcgcgcgtgcttgatgacgcgtatgacctgcttgtggacacccgccgccttgttttacctgtagttaccgatacaggccggcggcgtgggtggtagcgcctagaacggacgaagggcatatagcgtaacagcctacttcggccgcaaagtgatgagctgaNaeIBsrDIBssHIINgoMIVE126QC128RA130RA132HE134LL136RI138LD140EH142LW144AA146EQ148NG150HQ152WL154CP156AA158AP160TI162AD164LA166CF168PY170IA172LS174DE176AG178VS180LL182D2776-3450gst9 CDSgst9 gene3,1603,1803,2003,2203,2403,2603,2803,3003,320
    acccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgagccggcctcacttgcggaagaaacctgtcaataatactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccactgggccgccatgctgcgaccgacctggcacagtttgcgtaggggcctaagtggcagtacagcccctaaaaaggttggagtcgaaggaagctcggccggagtgaacgccttctttggacagttattatgatcatcgccggcgacgtcaggccgttttttcccgttccacagtggtgAjuIBplIBplINaeINotIPstISpeIAvaIIBbvCIPflFINgoMIVTsp45ITth111IY184PA186VR188RW190LD192RV194KR196IP198GF200TV202MS204GI206FP208TS210AS212FE214PA216SL218AE220ET222CQ224*2776-3450gst9 CDSgst9 geneE. coli His...TerminatorSuffix3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaggacgggaaaaagaaattttggcttttctaatgaagcgcaatacgtccgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgtttPciIBsrBIAflIIIE. coli His Operon TerminatorVR Primer3,5203,5403,5603,5803,6003,6203,6403,660
    aggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctccttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggarep (pMB1)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactcaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgaApaLIBseYIrep (pMB1)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcarep (pMB1)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    taccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattcccrep (pMB1)4,2204,2404,2604,2804,3004,3204,3404,360
    attttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagattaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcgagctccgaacctaagagtggttattttttgcgggccgccgttggctcgcaagacttgtttaggtctaPspXIT0 Terminator4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcacctcctcaagactccagtaatgacctagatagttgtcctcaggttcgctcgagctatagtttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaSacIEcoRVEco53kIChloramphenicol ResistanceT0 Terminator4,5604,5804,6004,6204,6404,6604,6804,7004,720
    tgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgNcoIPasIStyIBsmBIEsp3IPflMIChloramphenicol Resistance4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccAclIBspEIBsrDIChloramphenicol Resistance4,9204,9404,9604,9805,0005,0205,0405,060
    cgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatatagPvuIIChloramphenicol Resistance5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctgtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacgggctagttgagctcacggtggactgcagattctttggtaataatagtactgtaattggaBsaAIPspXIVF2 PrimerChlora...tance5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctgGAATTCGCGGCCGCTTCTAGAGtatttttatccgcatagtgctccgtcttaaagtctattttttttaggaatcgaaagcgattcctactaaagacCTTAAGCGCCGGCGAAGATCTCNotIXbaIEcoRIStem LoopiGEM15C-09Prefix5,4405,4605,4805,5005,520
    • Plasmid
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    5520 bp40004250450047505000525025050075010001250ndmD CDSndmD genendmC CDSEQ_NdmC_reviGEM15C-09947-271365-919

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    5520