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pAAV-hSyn-DIO-HA-hM4D-p2a-mCitrine-t2a-GIRK1
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagactgcagagggcggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcacacagatctgacgtctcccgEagIMluINotISbfIXbaIAflIIIL-ITRHuman S...omoter20406080100120140160
    cctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccggacgcatactcacgttcacccaaaatcctggtcctactccgccccacccccacggatggactgctggctggggctgggtgacctgttcgtgggttgggggtaaggggtttaacgcgtaggggatagtctctccccctcccctttgtcctacgccgctccgcgcacgcgtgacggFspAIHuman Synapsin promoter180200220240260280300320340
    agcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgctcgaagtcgtggcgcctgtcacggaagcgggggcggaccgccgcgcgcggtggcggcggagtcgtgacttccgcgcgactgcagtgagcggccagggggcgtttgaggggaagggccggtggaaccagcgcaggcgcggcggcggccgggtcggcctggcgtggtgcgctccgcgBbvCIRsrIIHuman Synapsin promoter360380400420440460480500520
    gagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgagaaggtaccggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagctctatccccccgtgcccgcgctggtagacgcgacgccgcggccgctgagtcgcgacggagtcagacgccacccgtcgcctcctcagcacagcacggactctcgcgtcagctcttccatggcctaggagatctcagctgaggccttattgaagcatatcctatgaaatatgcttcAccIKpnIMreISalIXbaIBamHIBspEISgrAIAcc65IHuman Synapsin promoterlox2722540560580600620640660680700
    ttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccctatgtgaagcggtcagagttcatttttcttagtttggctggaagattcccttccatcctggtaggtcctccagccatcaatacgtcttaccatcgacctaacatcgacgataatcgttatactttggagaattattgaagcatatcgtatgtaatatgcttcaataccgcgcgggatacacttcgccagtctcaagtaaaaagaatcaaaccgaccttctaagggaaggtaggaccatccaggaggtcggtagAscIPpuMIGIRK1 F137SloxP720740760780800820840860
    ttttgaagcttcggtggcaaatcggccacagactggctcatgggatctgataacatcttggtggttttagataccagtttttcttcagagttgccaggaaccgaacttattcgttggagtttcatgggcaaatcacccaaactataggctttttctgaagttgtagaactcatccaaaacttcgaagccaccgtttagccggtgtctgaccgagtaccctagactattgtagaaccaccaaaatctatggtcaaaaagaagtctcaacggtccttggcttgaataagcaacctcaaagtacccgtttagtgggtttgatatccgaaaaagacttcaacatcttgagtaggHindIIIGIRK1 F137S8809009209409609801,0001,0201,040
    tcaggagttttttgggaaagtcttctctccccgtaattttctgcagcttcgatggaagttttgtgctaatgtcatctagtccatctaagcactccacagaattgtgtctttctttgctgttggttatggctggtgctattaaaggggaagacatgagaagcatttcttcctgctcagtcctcaaaaaaccctttcagaagagaggggcattaaaagacgtcgaagctaccttcaaaacacgattacagtagatcaggtagattcgtgaggtgtcttaacacagaaagaaacgacaaccaataccgaccacgataatttccccttctgtactcttcgtaaagaaggacgagBsu36IGIRK1 F137S1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tttcacactgtacggaggggtggggacttcaaaggttgcatggaactgggagtaatcgactttaaagaatccttcttctaaagaaattacagggaaaaaacgatgaccccaaagaacttcatcttcggtgtatgatgttcgagcttgacaagtcatccctgtggtttccacgatgaaagtgtgacatgcctccccacccctgaagtttccaacgtaccttgaccctcattagctgaaatttcttaggaagaagatttctttaatgtcccttttttgctactggggtttcttgaagtagaagccacatactacaagctcgaactgttcagtagggacaccaaaggtgctacGIRK1 F137S1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ccttccaggatgacgaccacctcgaactgttcagtttgcatgcttcgctgggataggtcataaaaggggcttttggcatcgatcacgtggcaaatggtgagaggggacacaagaaaaagttgatctgcccctgtactaaaacctacatccagttcaagttggtcaaggggtagaaggaaggtcctactgctggtggagcttgacaagtcaaacgtacgaagcgaccctatccagtattttccccgaaaaccgtagctagtgcaccgtttaccactctcccctgtgttctttttcaactagacggggacatgattttggatgtaggtcaagttcaaccagttccccatcttPmlIGIRK1 F137S1,4201,4401,4601,4801,5001,5201,5401,560
    actcaccctcaggtgtctgccgagatttgagcagcttgcagcggatctgcgcggagaccatgtggctgttgcgcaggttgcccacccggaacatgagagtgagttttccgtccctcatggaaataaccgcatgctcgctaaacatgagggtctcggcgcgctttttgggctgggatgagtgggagtccacagacggctctaaactcgtcgaacgtcgcctagacgcgcctctggtacaccgacaacgcgtccaacgggtgggccttgtactctcactcaaaaggcagggagtacctttattggcgtacgagcgatttgtactcccagagccgcgcgaaaaacccgaccctBsu36IGIRK1 F137S1,5801,6001,6201,6401,6601,6801,7001,7201,740
    catcttgatgaacatgcagccgatgaggaaagcgtccacgatggagccaaggatggactggaaaaggaaaaggatgatgccctcggggcacttgtcggtgatgtagcggtagccatagccgatggtggcctcggtctcgatggagaagaggaaggcagaggggaagttatagacagtagaactacttgtacgtcggctactcctttcgcaggtgctacctcggttcctacctgaccttttccttttcctactacgggagccccgtgaacagccactacatcgccatcggtatcggctaccaccggagccagagctacctcttctccttccgtctccccttcaatatctgtXcmIGIRK1 F137S1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttggccacacagggagtgtagttgccgacatgggctttgttcaggtcgccccgggtataagcgatcacccaccacatggacgccatgaagagccaggccacggtgtaggtgaggatgaagataaagaggttccaacgccacttgagatccaccagggtagtgaagaggtcggaaaaaccggtgtgtccctcacatcaacggctgtacccgaaacaagtccagcggggcccatattcgctagtgggtggtgtacctgcggtacttctcggtccggtgccacatccactcctacttctatttctccaaggttgcggtgaactctaggtggtcccatcacttctccagcctttSapIBspQIGIRK1 F137S1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggtagcgactggtctcgctgcccaggttgccgtgctgcacattgcaccgaccgttcttgtccacgaaccgctgccgtttcttcttgggtacaagctgctgctgtgggccctgtcctggcccctggggctgcaagcccgaaccgctggacgaagtggtcactacctggtaatcgtcccatcgctgaccagagcgacgggtccaacggcacgacgtgtaacgtggctggcaagaacaggtgcttggcgacggcaaagaagaacccatgttcgacgacgacacccgggacaggaccggggaccccgacgttcgggcttggcgacctgcttcaccagtgatggaccattagcagSexAIGIRK1 F137S2,1202,1402,1602,1802,2002,2202,2402,260
    cccaaatttccttcggagtgcagacatgggcccagggttttcttcaacatctcctgcttgttttaaaagggagaaatttgtggccttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttggggtttaaaggaagcctcacgtctgtacccgggtcccaaaagaagttgtagaggacgaacaaaattttccctctttaaacaccggaacatgtcgagcaggtacggctctcactagggccgccgccagtgcttgaggtcgtcctggtacactagcgcgaagagcaaccccagaaacmCitrinep2AGIRK1 F137S2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctcagtttggactggtagctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagagagtcaaacctgaccatcgagtccatcaccaacagcccgtcgtcgtgccccggcagcggctacccccacaagacgaccatcaccagccgctcgacgtgcgacggcaggagctacaacaccgcctagaacttcaagtggaactacggcaagaagacgaacagccggtactatatctmCitrine2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagcaacaccgacaacatcaacatgaggtcgaacacggggtcctacaacggcaggaggaacttcagctacgggaagtcgagctacgccaagtggtcccacagcgggagcttgaagtggagccgcgcccagaacatcaacggcagcaggaacttcttctaccacgcgaggacctgcatmCitrine2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgggcgaagcacatcaggccgtagccgaaggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgcggaagcccgtaccgcctgaacttcttcagcacgacgaagtacaccagccccatcgcccgcttcgtgtagtccggcatcggcttccaccagtgctcccacccggtcccgtgcccgtcgaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcmCitrine2,8202,8402,8602,8802,9002,9202,9402,960
    ccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggggccgggattttcctccacgtccccgcatgttagaagacttcccctgccctccctggcagtgccgatgttccgggagcggcctgtgcgacttgaacaccggcaaatgcagcggcaggtcgagctggtcctacccgtggtggggccacttgtcgaggagcgggaacgagtggtaccccggccctaaaaggaggtgcaggggcgtacaatcttctgaaggggacgggagggaccgtcacggctacaaggNcoIBmgBImCitrinet2AhM4D2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gatactggcacagcagcaggtgccggaaggtctttttaaaggtggcgttgcacagagcatagcaggcagggttgatggtgctgttgacgtagcagagccagtagccaatggaccacaccgtgtcagggatgcagctctggcagaaggtgttcaccaggaccatgacgttgtagggctatgaccgtgtcgtcgtccacggccttccagaaaaatttccaccgcaacgtgtctcgtatcgtccgtcccaactaccacgacaactgcatcgtctcggtcatcggttacctggtgtggcacagtccctacgtcgagaccgtcttccacaagtggtcctggtactgcaacatcccPaqCIBstAPIDraIIIhM4D3,1603,1803,2003,2203,2403,2603,2803,3003,320
    cgtccaggtgaggatgaaggctggcagaatggcaaagatcgttcgtgtcactttgcgctcccgggccgccatctgccgcttcttgcgcacctggttgcgagcgatgctggcgaacttgcgggccacgttggccgcagggcgcatgccagccggcgtggcaggcacaatctcaatggcaggtccactcctacttccgaccgtcttaccgtttctagcaagcacagtgaaacgcgagggcccggcggtagacggcgaagaacgcgtggaccaacgctcgctacgaccgcttgaacgcccggtgcaaccggcgtcccgcgtacggtcggccgcaccgtccgtgttagagttacSfiISexAIhM4D3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gctgtcacacactcattgcctgtctgcttcgtcacaatctggatcttggaccatctggaggctgggttgagggcccgcggctgcaggggaggggcgggcatggcgggcgtggtggcctctgtggtggacagctctgtggctgggcgttccttggtgttctgggtggcactgcctgcgacagtgtgtgagtaacggacagacgaagcagtgttagacctagaacctggtagacctccgacccaactcccgggcgccgacgtcccctccccgcccgtaccgcccgcaccaccggagacaccacctgtcgagacaccgacccgcaaggaaccacaagacccaccgtgacggacXcmIhM4D3,5203,5403,5603,5803,6003,6203,6403,660
    agctggactcattggaagtgtccttatcagccacggggcgcggtggcggtggcagcgctggcgggggggcctcctccagcttgccattgcgcagctcctcccgggcggcctccccgggcgggggcttcttgacgctctgcttcattagtgggctcttgaggaaggccagcgtctttcgacctgagtaaccttcacaggaatagtcggtgccccgcgccaccgccaccgtcgcgaccgcccccccggaggaggtcgaacggtaacgcgtcgaggagggcccgccggaggggcccgcccccgaagaactgcgagacgaagtaatcacccgagaactccttccggtcgcagaahM4D3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggctttcttctccttcgggccctcgggccggtgcttgtggactcggctgcgactggccagggagatgtggatgtacagcaccgtcatgatgaccacaggcaggtagaagccagcaatggctgtgccaaaggtcactgctgggttggacaggaactggatgaagcactggttgtcgccgaaagaagaggaagcccgggagcccggccacgaacacctgagccgacgctgaccggtccctctacacctacatgtcgtggcagtactactggtgtccgtccatcttcggtcgttaccgacacggtttccagtgacgacccaacctgtccttgacctacttcgtgaccaacagcAlwNIhM4D3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggcaccgtccgcttacccaccacaaactgccagaacaagatggcaggcgcccagagcacgaaggacagtacccaggcagcagcaatcatgaggcctgccatcttggtggtgcgccgggcagggtaggtgagaggcttggtgacgcagaagtagcggtcaaagctgatgatgagaaccgtggcaggcgaatgggtggtgtttgacggtcttgttctaccgtccgcgggtctcgtgcttcctgtcatgggtccgtcgtcgttagtactccggacggtagaaccaccacgcggcccgtcccatccactctccgaaccactgcgtcttcatcgccagtttcgactactactcttStuIhM4D4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggttcatgacggaggcgttgctcaccacgcagtccagggccagccacaggtcgcagaccacggcgcccaggggccagtagcccttgatgatgtacacggtgtagaggttcatggagaaggcgcctatgatgagatcagcacacgccaggctgaagaggaagtagttgttgactgtccaagtactgcctccgcaacgagtggtgcgtcaggtcccggtcggtgtccagcgtctggtgccgcgggtccccggtcatcgggaactactacatgtgccacatctccaagtacctcttccgcggatactactctagtcgtgtgcggtccgacttctccttcatcaacaactgacahM4D4,2204,2404,2604,2804,3004,3204,3404,360
    ctgcagctgcctgttgaccttgatggacagcatcaccaggatgttgcccacgacagtcaccaggctcagggagcctgtcactgtggcaatgaagaccatttccaccgtctcatagcgattgtgggatgatgacgtgaccaggcgcacggactgattgcccgagctgccattgacagacgtcgacggacaactggaactacctgtcgtagtggtcctacaacgggtgctgtcagtggtccgagtccctcggacagtgacaccgttacttctggtaaaggtggcagagtatcgctaacaccctactactgcactggtccgcgtgcctgactaacgggctcgacggtaactgtBmgBIhM4D4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggtgtgaagttggccatagcgtaatctggaacatcgtatgggtacatggtggctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatccacacttcaaccggtatcgcattagaccttgtagcatacccatgtaccaccgatcgtattgaagcatatttcataggatatgcttcaataaacggaattgggtctttaatagtgacaataagaaatcttaccacgtttcttattgaagcatattacatacgatatgcttcaataBmtINheInhe1-Kozak-HAlox2722loxPhM4D4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaEcoRIEcoRVHindIIIWPRE4,7404,7604,7804,8004,8204,8404,8604,8804,900
    cctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcgWPRE4,9204,9404,9604,9805,0005,0205,0405,060
    ctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttccgacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttagtagcaggaaaggaaccgacgagcggatacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaaggWPRE5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccacgcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgTliIXhoIBglIIPaeR7IWPREhGH polyA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcaaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatattataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtCspCIhGH polyA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    caatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcagggttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccBlpINsiIBbvCIhGH polyA5,6205,6405,6605,6805,7005,7205,7405,760
    tgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaagactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccAloIEagINotIPmlIRsrIIBstEIIhGH polyAR-ITR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaaggctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcSbfIR-ITR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctgf1 Origin6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgagggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctAloIPsiIDraIIIf1 Origin6,3206,3406,3606,3806,4006,4206,4406,460
    tttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaac6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaa6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcgcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaagAmpR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtgacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccaAmpR7,0207,0407,0607,0807,1007,1207,1407,160
    attatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttactttaatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaaScaIAmpR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcggactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcPvuIAmpR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    aactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcggAseIAmpR7,5407,5607,5807,6007,6207,6407,6607,6807,700
    ctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatcAmpR7,7207,7407,7607,7807,8007,8207,8407,860
    gtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacgpUC ori7,8807,9007,9207,9407,9607,9808,0008,0208,040
    cggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgagcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctAlwNIpUC ori8,0608,0808,1008,1208,1408,1608,1808,2008,220
    taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaagattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttcpUC ori8,2408,2608,2808,3008,3208,3408,3608,3808,400
    gcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcpUC ori8,4208,4408,4608,4808,5008,5208,5408,560
    cggcctttttacggttcctggccttttgctggccttttgctcacatgtgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori8,5808,6008,620
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    8623 bp60006500700075008000500100015002000GIRK1 F137S

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    8623